Expression of endogenous ouabain in placenta and the concentrations of serum ET-1 and NO were examined in 30 patients with hypertensive disorder complicating pregnancy （HDCP） and 30 healthy pregnant women to investi...Expression of endogenous ouabain in placenta and the concentrations of serum ET-1 and NO were examined in 30 patients with hypertensive disorder complicating pregnancy （HDCP） and 30 healthy pregnant women to investigate the effect of endogenous ouabain on HDCP. Compared with the healthy pregnant group, the expression of endogenous ouabain dramatically increased in the HDCP groups （P〈0.01）. There was a significantly positive correlation between the expression of en- dogenous ouabain with ET-1 （r= 0.5567, P〈0.01）, while the correlation of endogenous ouabain and NO was significantly negative （r=-0.6895, P〈0.01）. As expected, the correlation between ET-1 and NO was negative （r=-0.7796, P〈0.01）. ET-1 concentrations of maternal and cord sera in HDCP groups were significantly higher in comparison with healthy pregnant group （P〈0.01）. On the contrast, NO concentrations were much lower in the maternal and cord sera of HDCP groups as compared with healthy pregnant group （P〈0.01）. Our data suggest that endogenous ouabain is directly involved in the nosogenesis of HDCP, with accompanying decreased NO and the elevated of ET-1.展开更多
Objective To explore the relationship between endogenous ouabain and the pathogenesis of hypertension. Methods ① Sixteen Sprague Dawley (SD) rats were selected and randomly divided into two groups, and then the rats ...Objective To explore the relationship between endogenous ouabain and the pathogenesis of hypertension. Methods ① Sixteen Sprague Dawley (SD) rats were selected and randomly divided into two groups, and then the rats were injected with ouabain in dosage of 20μg/kg.d and normal saline (NS), respectively. The indirect systolic blood pressure of all the rats were recorded once a week. ② Twenty five 1k1c (one kidney and one clipped) hypertensive rats were established and injected randomly with anti ouabain antibody, normal rabbit IgG and normal saline, respectively. The direct blood pressure of all the 1klc hypertensive rats were recorded by cannulated carotid artery for 3h after administration. Results The systolic blood pressure of rats injected with ouabain began to increase 2 weeks after ouabain administration and increased significantly at 6 weeks compared with NS group (17.7±1.2)kPa vs (15.4±1.1)kPa, P <0.01).Anti ouabain antibody could significantly decrease the blood pressure of 1k1c rats, while the normal rabbit IgG could not. Conclusion The results of this study indicate that endogenous ouabain might be one of the pathogenetic factors of hypertension.展开更多
Objective To compare the characteristics or endogenous ouabaln(EO) secretion with the other adrenocortical hormones and determine the effects of angiotensin I (Ang I ), and ad reno corticotrophin (ACTH ) on the secret...Objective To compare the characteristics or endogenous ouabaln(EO) secretion with the other adrenocortical hormones and determine the effects of angiotensin I (Ang I ), and ad reno corticotrophin (ACTH ) on the secretion or EO. Methods EO was measured by radioimmunoassay from primary cultured bovine adrenocotical cells (BAC). Results oouabain was determined in the media or cultured BAC. Both EO and aldosterone secretion were decreased from the 6uter to inner layer or the cultured adrenal cortex, and the responses to Ang I and ACTH were hlgher than that in the mld layer (P <o. o5) and inner layer (P <o. o1 ). Cortisol secretion was activated by Ang I or ACTH was slgnificantly higher in the mid layer and in the inner layer than that in the outer layer. The tlme-course experlment showed that the gradually rising amounts or aldosterone and cortisol could be determined during the continuous incubation to 48h wlth or without Ang I or ACTH. However, EO did not increase continuously arter 24h or incubation in the basal secreting sltuation and arter 12h of lncubatiou in the stimulating situation by Ang,or ACTH. There were obvlous drops in aldosterone and cortisol secretlou from 3rd day during a 21 day-perlod cell culture, but the peak secretion of ouabain was in 7th day. Conclusion it suggests that the secretory mechanism might be different between EO and aldosterone or cortisol. Also, Ang I and ACTR might be involved in the regulation of Eo secretion.展开更多
To evaluate the role of endogenous ouabain (EO) in the development of hypertension and the characteristics of EO secretion in 1k1c (one kidney, one clipped) hypertensive rats Methods EO content of serum and tissues...To evaluate the role of endogenous ouabain (EO) in the development of hypertension and the characteristics of EO secretion in 1k1c (one kidney, one clipped) hypertensive rats Methods EO content of serum and tissues in 1k1c hypertensive rats and normal control Sprague Dawley (SD) rats was detected by the method of enzyme linked immunosorbent assay (ELISA) The relationship between serum or tissue ouabain and blood pressure was analyzed in 1k1c hypertensive rats Results The ouabain content of serum, heart, kidney, adrenal gland, pituitary and hypothalamus was significantly higher in 1k1c hypertensive rats than that in normal control SD rats (2 25, 2 63, 3 35, 40 37, 3 34, 15 7?μg/kg tissue in 1k1c hypertensive rats vs 1 12, 1 79, 1 73, 27 54, 1 83, 10 10?μg/kg tissue in control SD rats, respectively P <0 05 for all of these comparisons) The ouabain content of the adrenal gland and the hypothalamus was higher than that of other tissues or serum, both in 1k1c rats and in control SD rats The EO content of serum, kidney and hypothalamus was significantly correlated with blood pressure in 1k1c hypertensive rats ( r =0 59, 0 63, 0 52, respectively. P <0 05) The ouabain content of heart, liver, adrenal gland and pituitary was not correlated with blood pressure Conclusions EO might play an important role in the development of hypertension in 1k1c hypertensive rats The adrenal gland may be a major source of EO and the hypothalamus pituitary adrenal axis may be involved in the regulation of EO secretion展开更多
基金the Scientific Research Foundation for the Returned Overseas ChineseScholars, State Education Ministry (NO:200414519001).
文摘Expression of endogenous ouabain in placenta and the concentrations of serum ET-1 and NO were examined in 30 patients with hypertensive disorder complicating pregnancy （HDCP） and 30 healthy pregnant women to investigate the effect of endogenous ouabain on HDCP. Compared with the healthy pregnant group, the expression of endogenous ouabain dramatically increased in the HDCP groups （P〈0.01）. There was a significantly positive correlation between the expression of en- dogenous ouabain with ET-1 （r= 0.5567, P〈0.01）, while the correlation of endogenous ouabain and NO was significantly negative （r=-0.6895, P〈0.01）. As expected, the correlation between ET-1 and NO was negative （r=-0.7796, P〈0.01）. ET-1 concentrations of maternal and cord sera in HDCP groups were significantly higher in comparison with healthy pregnant group （P〈0.01）. On the contrast, NO concentrations were much lower in the maternal and cord sera of HDCP groups as compared with healthy pregnant group （P〈0.01）. Our data suggest that endogenous ouabain is directly involved in the nosogenesis of HDCP, with accompanying decreased NO and the elevated of ET-1.
基金This project was supported by the National Medicine Technique Innovation Foundation of ChinaNo.98-B-0 7
文摘Objective To explore the relationship between endogenous ouabain and the pathogenesis of hypertension. Methods ① Sixteen Sprague Dawley (SD) rats were selected and randomly divided into two groups, and then the rats were injected with ouabain in dosage of 20μg/kg.d and normal saline (NS), respectively. The indirect systolic blood pressure of all the rats were recorded once a week. ② Twenty five 1k1c (one kidney and one clipped) hypertensive rats were established and injected randomly with anti ouabain antibody, normal rabbit IgG and normal saline, respectively. The direct blood pressure of all the 1klc hypertensive rats were recorded by cannulated carotid artery for 3h after administration. Results The systolic blood pressure of rats injected with ouabain began to increase 2 weeks after ouabain administration and increased significantly at 6 weeks compared with NS group (17.7±1.2)kPa vs (15.4±1.1)kPa, P <0.01).Anti ouabain antibody could significantly decrease the blood pressure of 1k1c rats, while the normal rabbit IgG could not. Conclusion The results of this study indicate that endogenous ouabain might be one of the pathogenetic factors of hypertension.
文摘Objective To compare the characteristics or endogenous ouabaln(EO) secretion with the other adrenocortical hormones and determine the effects of angiotensin I (Ang I ), and ad reno corticotrophin (ACTH ) on the secretion or EO. Methods EO was measured by radioimmunoassay from primary cultured bovine adrenocotical cells (BAC). Results oouabain was determined in the media or cultured BAC. Both EO and aldosterone secretion were decreased from the 6uter to inner layer or the cultured adrenal cortex, and the responses to Ang I and ACTH were hlgher than that in the mld layer (P <o. o5) and inner layer (P <o. o1 ). Cortisol secretion was activated by Ang I or ACTH was slgnificantly higher in the mid layer and in the inner layer than that in the outer layer. The tlme-course experlment showed that the gradually rising amounts or aldosterone and cortisol could be determined during the continuous incubation to 48h wlth or without Ang I or ACTH. However, EO did not increase continuously arter 24h or incubation in the basal secreting sltuation and arter 12h of lncubatiou in the stimulating situation by Ang,or ACTH. There were obvlous drops in aldosterone and cortisol secretlou from 3rd day during a 21 day-perlod cell culture, but the peak secretion of ouabain was in 7th day. Conclusion it suggests that the secretory mechanism might be different between EO and aldosterone or cortisol. Also, Ang I and ACTR might be involved in the regulation of Eo secretion.
文摘To evaluate the role of endogenous ouabain (EO) in the development of hypertension and the characteristics of EO secretion in 1k1c (one kidney, one clipped) hypertensive rats Methods EO content of serum and tissues in 1k1c hypertensive rats and normal control Sprague Dawley (SD) rats was detected by the method of enzyme linked immunosorbent assay (ELISA) The relationship between serum or tissue ouabain and blood pressure was analyzed in 1k1c hypertensive rats Results The ouabain content of serum, heart, kidney, adrenal gland, pituitary and hypothalamus was significantly higher in 1k1c hypertensive rats than that in normal control SD rats (2 25, 2 63, 3 35, 40 37, 3 34, 15 7?μg/kg tissue in 1k1c hypertensive rats vs 1 12, 1 79, 1 73, 27 54, 1 83, 10 10?μg/kg tissue in control SD rats, respectively P <0 05 for all of these comparisons) The ouabain content of the adrenal gland and the hypothalamus was higher than that of other tissues or serum, both in 1k1c rats and in control SD rats The EO content of serum, kidney and hypothalamus was significantly correlated with blood pressure in 1k1c hypertensive rats ( r =0 59, 0 63, 0 52, respectively. P <0 05) The ouabain content of heart, liver, adrenal gland and pituitary was not correlated with blood pressure Conclusions EO might play an important role in the development of hypertension in 1k1c hypertensive rats The adrenal gland may be a major source of EO and the hypothalamus pituitary adrenal axis may be involved in the regulation of EO secretion
