In this experiment,we have observed the effects of acupuncture on the contents ofenkephalins in different brain regions and corticosterone in plasma of rats with endotoxin shock.Theresults showed that after acupunctur...In this experiment,we have observed the effects of acupuncture on the contents ofenkephalins in different brain regions and corticosterone in plasma of rats with endotoxin shock.Theresults showed that after acupuncture the endogenous enkephalins were significantly decreased(P【0.05)in the midbrain and brain stem of endotoxin shock rats,suggesting the anti-shock of acupuncture might be related to the enkephalins in the midbrain and brain stem.In addition,there was a rising trend in plasma corticosterone of the rats with toxic shock.展开更多
Objective: To compare the protective effects of removingcirculatory TNF by specific immunoadsorption and by nonspeciflc Amberlite XAD-7 adsorption on experimental endotoxin shock. Methods: New Zealand white rabbits re...Objective: To compare the protective effects of removingcirculatory TNF by specific immunoadsorption and by nonspeciflc Amberlite XAD-7 adsorption on experimental endotoxin shock. Methods: New Zealand white rabbits receiving a lethal dose of endotoxin underwent hemoperfusion through immunoadsorbent or Amberlite BAD-7. Plasma TNF levels. efficiency of the adsorbents and the survival rate were observed. Results: After 2 h of hemopcrfusion through immunoadsorhent or Amberlite XAD-7. plasma TNF levels were significantly lower than those in the control group(P<0.01). and the best result was shown by immunoadsorption (P<0. 01). The 12 h and 18 h survival rates were 70% and 30% in the immunoadsorption group, and 30% and 5% in the Amberlite XAD-7 group respectively (P<0. 05). Conclusion: Compared with the nonspecific Amberlite XAD-7 adsorption,the specific imrnunoadsorption might be a more effective method of removing circulatory TNF and improve the survival rate in endotoxin shock.展开更多
Objective:To examine the effects of modified Shenmai Yin on invigorating vital energy, promoting blood flow, and protection against neural impairment in an endotoxin-induced shock rat model. Methods: Ninety-six SD rat...Objective:To examine the effects of modified Shenmai Yin on invigorating vital energy, promoting blood flow, and protection against neural impairment in an endotoxin-induced shock rat model. Methods: Ninety-six SD rats were randomly divided into four groups: sham operation (saline 20 ml/kg), shock model (lipopolysaccharide, LPS, 8 mg/kg), Reformed Shengmai Yin (加味生脉饮 Pulse-activating Decoction) (LPS 8 mg/kg + reformed Shengmai Yin Injection 10 ml/kg), and dexamethasone (LPS 8 mg/kg + dexamethasone 5 mg/kg) groups. Each group was subdivided into 1 h, 2 h, 3 h, and 6 h time points for observation. The carotid artery was separated and connected with a biological functional system to monitor mean arterial pressure (MAP). Brain water levels, malonaldehyde (MDA) content, and superoxide dismutase (SOD) activity were also determined. Results: In the shock model group, MAP was progressively decreased after injection of LPS, brain water and MDA contents were increased, brain SOD activity was decreased, and capillary vessel edema in brain tissue was also observed. All these parameters were improved significantly in both treatment groups, although the effects were more marked with Shengmai Yin than with dexamethasone. Conclusion: Modified Shengmai Yin exhibits strong anti-shock and neuroprotective effects against Endotoxininduced shock.展开更多
Objective:To investigate the effects of CCK-8 on mean pulmonary artery pressure in rats with endotoxic shock.Methods:Male SD rats were randomized into seven groups(n=6):control group,model group,LPS+CCK-8 group,CCK-8 ...Objective:To investigate the effects of CCK-8 on mean pulmonary artery pressure in rats with endotoxic shock.Methods:Male SD rats were randomized into seven groups(n=6):control group,model group,LPS+CCK-8 group,CCK-8 group,CCK-1R antagonist group,CCK-2R antagonist group,DFSO+PF group.The rats were induced to lethal endotoxic shock by an injection of LPS(30 mg.kg-1).CCK-8(50μg.kg-1)was administered 30 min after LPS injection.Either a specific CCK-1R antagonist or CCK-2R antagonist was injected before CCK-8 treatment.The mean arterial pressure(MAP)and mean pulmonary artery pressure(MPAP)were collected by a multi-channel data physiological recorder.As well as the 8h mortality was recorded.Results:Compared with control group,the MAP were significantly continuously lower and the MPAP significantly higher in model group.Administration of CCK-8 significantly delayed the LPS-induced not only decreases in MAP but also rises in MPAP,while reduceing the mortality.In addition,the specific antagonist at the CCK-2 receptor(CCK-2R)abrogated the action of CCK-8 significantly.Conclusion:while the LPS-induced hypotension delayed,CCK-8 could effectively alleviate the LPS-induced rises in MPAP via the CCK-2 receptor in ES rat model,while reduceing the mortality.展开更多
As compared with the control,the malondialdehyde(MDA)levels in the cardi-ac,pulmonary,and intestinal tissues were elevated(P【0.05),and the superoxide dismu-tase activities in the cardiac,pulmonary,and intestinal tiss...As compared with the control,the malondialdehyde(MDA)levels in the cardi-ac,pulmonary,and intestinal tissues were elevated(P【0.05),and the superoxide dismu-tase activities in the cardiac,pulmonary,and intestinal tissues increased(P【0.05)at the30th min after endotoxic shock;the former in the cardiac,hepatic,renal,pulmonary,andintestinal tissues were elevated(P【0.05～0.001)and the latter in the cardiac,pulmona-ry,and intestinal tissues decreased in the 2nd h after shock;and the former were marke-dly elevated(P【0.01～0.001)and the latter severely decreased(P【【0.05～0.001)in allthe visceral tissues tested in the 4th h after shock.These data showed that lipid peroxid-ation mediated through oxygen-derived free radicals brought about tissue damage of theviscera listed above,which occurred earlier in the heart,the lungs and the intestines thanin the liver and the kidneys,and superoxide dismutase(SOD)activity was suppressed inthe late stage of endotoxic shock.展开更多
Effects of bactericidal/permeability-increa protein(BPI, 1. 5 and 3. 5 rug/kg iv at the beginning of shock and resuscitation respectively) on the outcome of hemorrhagic shock was investigated in rats. Itwas found that...Effects of bactericidal/permeability-increa protein(BPI, 1. 5 and 3. 5 rug/kg iv at the beginning of shock and resuscitation respectively) on the outcome of hemorrhagic shock was investigated in rats. Itwas found that BPI administration could improve hepatic and renal functions after hemorrhagic shock, andenhance the survival rate of the rats with shock (BPI group: 81 % vs physiological saline group: 44 %, P <0.05). In BPI group, the plasma endotoxin level was not significantly changed (0. 20± 0. 04 at the end of resuscitation vs 0. 24± 0. 05 U/ml before shock). Tumor necrosis factor. and interleukin-6 levels in BPI group, although higher after shock and resuscitation, were significantly lower than those in physiologia saline group.It is suggested that BPI exerts a protective effects on rats with hernorrhagic shock, which might be due to itsaction against hemorrhage-induced endotoxin translocation and its inhibition of cytokine responses in shock,展开更多
基金This work was supported by the National fund of Natural Science.
文摘In this experiment,we have observed the effects of acupuncture on the contents ofenkephalins in different brain regions and corticosterone in plasma of rats with endotoxin shock.Theresults showed that after acupuncture the endogenous enkephalins were significantly decreased(P【0.05)in the midbrain and brain stem of endotoxin shock rats,suggesting the anti-shock of acupuncture might be related to the enkephalins in the midbrain and brain stem.In addition,there was a rising trend in plasma corticosterone of the rats with toxic shock.
文摘Objective: To compare the protective effects of removingcirculatory TNF by specific immunoadsorption and by nonspeciflc Amberlite XAD-7 adsorption on experimental endotoxin shock. Methods: New Zealand white rabbits receiving a lethal dose of endotoxin underwent hemoperfusion through immunoadsorbent or Amberlite BAD-7. Plasma TNF levels. efficiency of the adsorbents and the survival rate were observed. Results: After 2 h of hemopcrfusion through immunoadsorhent or Amberlite XAD-7. plasma TNF levels were significantly lower than those in the control group(P<0.01). and the best result was shown by immunoadsorption (P<0. 01). The 12 h and 18 h survival rates were 70% and 30% in the immunoadsorption group, and 30% and 5% in the Amberlite XAD-7 group respectively (P<0. 05). Conclusion: Compared with the nonspecific Amberlite XAD-7 adsorption,the specific imrnunoadsorption might be a more effective method of removing circulatory TNF and improve the survival rate in endotoxin shock.
基金supported by a grant from National Natural Science Foundation of China (No.30672737)
文摘Objective:To examine the effects of modified Shenmai Yin on invigorating vital energy, promoting blood flow, and protection against neural impairment in an endotoxin-induced shock rat model. Methods: Ninety-six SD rats were randomly divided into four groups: sham operation (saline 20 ml/kg), shock model (lipopolysaccharide, LPS, 8 mg/kg), Reformed Shengmai Yin (加味生脉饮 Pulse-activating Decoction) (LPS 8 mg/kg + reformed Shengmai Yin Injection 10 ml/kg), and dexamethasone (LPS 8 mg/kg + dexamethasone 5 mg/kg) groups. Each group was subdivided into 1 h, 2 h, 3 h, and 6 h time points for observation. The carotid artery was separated and connected with a biological functional system to monitor mean arterial pressure (MAP). Brain water levels, malonaldehyde (MDA) content, and superoxide dismutase (SOD) activity were also determined. Results: In the shock model group, MAP was progressively decreased after injection of LPS, brain water and MDA contents were increased, brain SOD activity was decreased, and capillary vessel edema in brain tissue was also observed. All these parameters were improved significantly in both treatment groups, although the effects were more marked with Shengmai Yin than with dexamethasone. Conclusion: Modified Shengmai Yin exhibits strong anti-shock and neuroprotective effects against Endotoxininduced shock.
文摘目的探讨oXiris-内毒素吸附技术治疗终末期肾病(end-stage renal disease,ESRD)合并脓毒症的疗效。方法回顾性收集2020年1月1日—2023年7月30日于河南科技大学第一附属医院住院治疗的ESRD合并脓毒症患者41例,其中oXiris组22例,连续性静脉-静脉血液滤过(continuous veno-venous hemofiltration,CVVH)联合血液灌流(hemoperfusion,HP)组19例。比较2组患者住院死亡率、ICU住院时长、机械通气时长、连续性肾脏替代治疗时长、机械通气患者数、使用滤器数以及在血液净化治疗开始时及治疗后急性生理与慢性健康评分(acute physiology and chronic health evaluation,APACHE-Ⅱ)、去甲肾上腺素(norepinephrine,NE)用量以及实验室检查结果。结果oXiris组患者机械通气时长少于CVVH+HP组(Z=-3.749,P=0.001)。治疗后oXiris组APACHE-Ⅱ评分(t=-4.481,P=0.001)、NE用量(t=-2.036,P=0.049)、C反应蛋白(Z=-2.850,P=0.004)、白细胞介素-6(Z=-2.512,P=0.012)水平较CVVH+HP组降低。结论oXiris-内毒素吸附技术能有效降低ESRD合并脓毒症患者炎症介质,且效果优于CVVH+HP,具有良好的临床应用价值。
基金Research Project of Hebei Administration of Traditional Chinese Medicine(No.2017005)Youth Fund of Hebei University of Chinese Medicine(No.QNZ2014036)
文摘Objective:To investigate the effects of CCK-8 on mean pulmonary artery pressure in rats with endotoxic shock.Methods:Male SD rats were randomized into seven groups(n=6):control group,model group,LPS+CCK-8 group,CCK-8 group,CCK-1R antagonist group,CCK-2R antagonist group,DFSO+PF group.The rats were induced to lethal endotoxic shock by an injection of LPS(30 mg.kg-1).CCK-8(50μg.kg-1)was administered 30 min after LPS injection.Either a specific CCK-1R antagonist or CCK-2R antagonist was injected before CCK-8 treatment.The mean arterial pressure(MAP)and mean pulmonary artery pressure(MPAP)were collected by a multi-channel data physiological recorder.As well as the 8h mortality was recorded.Results:Compared with control group,the MAP were significantly continuously lower and the MPAP significantly higher in model group.Administration of CCK-8 significantly delayed the LPS-induced not only decreases in MAP but also rises in MPAP,while reduceing the mortality.In addition,the specific antagonist at the CCK-2 receptor(CCK-2R)abrogated the action of CCK-8 significantly.Conclusion:while the LPS-induced hypotension delayed,CCK-8 could effectively alleviate the LPS-induced rises in MPAP via the CCK-2 receptor in ES rat model,while reduceing the mortality.
基金This study was supported by the National Natural Science Fundation of China
文摘As compared with the control,the malondialdehyde(MDA)levels in the cardi-ac,pulmonary,and intestinal tissues were elevated(P【0.05),and the superoxide dismu-tase activities in the cardiac,pulmonary,and intestinal tissues increased(P【0.05)at the30th min after endotoxic shock;the former in the cardiac,hepatic,renal,pulmonary,andintestinal tissues were elevated(P【0.05～0.001)and the latter in the cardiac,pulmona-ry,and intestinal tissues decreased in the 2nd h after shock;and the former were marke-dly elevated(P【0.01～0.001)and the latter severely decreased(P【【0.05～0.001)in allthe visceral tissues tested in the 4th h after shock.These data showed that lipid peroxid-ation mediated through oxygen-derived free radicals brought about tissue damage of theviscera listed above,which occurred earlier in the heart,the lungs and the intestines thanin the liver and the kidneys,and superoxide dismutase(SOD)activity was suppressed inthe late stage of endotoxic shock.
文摘Effects of bactericidal/permeability-increa protein(BPI, 1. 5 and 3. 5 rug/kg iv at the beginning of shock and resuscitation respectively) on the outcome of hemorrhagic shock was investigated in rats. Itwas found that BPI administration could improve hepatic and renal functions after hemorrhagic shock, andenhance the survival rate of the rats with shock (BPI group: 81 % vs physiological saline group: 44 %, P <0.05). In BPI group, the plasma endotoxin level was not significantly changed (0. 20± 0. 04 at the end of resuscitation vs 0. 24± 0. 05 U/ml before shock). Tumor necrosis factor. and interleukin-6 levels in BPI group, although higher after shock and resuscitation, were significantly lower than those in physiologia saline group.It is suggested that BPI exerts a protective effects on rats with hernorrhagic shock, which might be due to itsaction against hemorrhage-induced endotoxin translocation and its inhibition of cytokine responses in shock,
