Methylobacterium species,the representative bacteria distributed in phyllosphere region of plants,often synthesize carotenoids to resist harmful UV radiations.Methylobacterium extorquens is known to produce a caroteno...Methylobacterium species,the representative bacteria distributed in phyllosphere region of plants,often synthesize carotenoids to resist harmful UV radiations.Methylobacterium extorquens is known to produce a carotenoid pigment and recent research revealed that this carotenoid has a C_(30) backbone.However,its exact structure remains unknown.In the present study,the carotenoid produced by M.extorquens AM1 was isolated and its structure was determined as 4-[2-O-11Z-octadecenoyl-β-glucopyranosyl]-4,4′-diapolycopenedioc acid(1),a glycosylated C_(30) carotenoid.Furthermore,the genes related to the C_(30)carotenoid synthesis were investigated.Squalene,the precursor of the C_(30) carotenoid,is synthesized by the co-occurrence of META1p1815,META1p1816 and META1p1817.Further overexpression of the genes related to squalene synthesis improved the titer of carotenoid 1.By using gene deletion and gene complementation experiments,the glycosyltransferase META1p3663 and acyltransferase META1p3664 were firstly confirmed to catalyze the tailoring steps from 4,4′-diapolycopene-4,4′-dioic acid to carotenoid 1.In conclusion,the structure and biosynthetic genes of carotenoid 1 produced by M.extorquens AM1 were firstly characterized in this work,which shed lights on engineering M.extorquens AM1 for producing carotenoid 1 in high yield.展开更多
Methanol has recently gained significant attention as a potential carbon substrate for the production of fuels and chemicals,owing to its high degree of reduction,abundance,and low price.Native methylotrophic yeasts a...Methanol has recently gained significant attention as a potential carbon substrate for the production of fuels and chemicals,owing to its high degree of reduction,abundance,and low price.Native methylotrophic yeasts and bacteria have been investigated for the production of fuels and chemicals.Alternatively,synthetic methylotrophic strains are also being developed by reconstructing methanol utilization pathways in model microorganisms,such as Escherichia coli.Owing to the complex metabolic pathways,limited availability of genetic tools,and methanol/formaldehyde toxicity,the high-level production of target products for industrial applications are still under development to satisfy commercial feasibility.This article reviews the production of biofuels and chemicals by native and synthetic methylotrophic microorganisms.It also highlights the advantages and limitations of both types of methylotrophs and provides an overview of ways to improve their efficiency for the production of fuels and chemicals from methanol.展开更多
基金the National Key R&D Program of China(grant No.2021YFC2103500)National Natural Science Foundation of China(grant No.22078169)+1 种基金Natural Science Foundation of Shandong Province,China(ZR2021MC074,ZR2020MC008)Shandong Provincial Key Research and Development Plan(2021ZDSYS28).
文摘Methylobacterium species,the representative bacteria distributed in phyllosphere region of plants,often synthesize carotenoids to resist harmful UV radiations.Methylobacterium extorquens is known to produce a carotenoid pigment and recent research revealed that this carotenoid has a C_(30) backbone.However,its exact structure remains unknown.In the present study,the carotenoid produced by M.extorquens AM1 was isolated and its structure was determined as 4-[2-O-11Z-octadecenoyl-β-glucopyranosyl]-4,4′-diapolycopenedioc acid(1),a glycosylated C_(30) carotenoid.Furthermore,the genes related to the C_(30)carotenoid synthesis were investigated.Squalene,the precursor of the C_(30) carotenoid,is synthesized by the co-occurrence of META1p1815,META1p1816 and META1p1817.Further overexpression of the genes related to squalene synthesis improved the titer of carotenoid 1.By using gene deletion and gene complementation experiments,the glycosyltransferase META1p3663 and acyltransferase META1p3664 were firstly confirmed to catalyze the tailoring steps from 4,4′-diapolycopene-4,4′-dioic acid to carotenoid 1.In conclusion,the structure and biosynthetic genes of carotenoid 1 produced by M.extorquens AM1 were firstly characterized in this work,which shed lights on engineering M.extorquens AM1 for producing carotenoid 1 in high yield.
基金the C1 Gas Refinery Program through the National Research Foundation of Korea(NRF),funded by the Ministry of Science and ICT(2015M3D3A1A01064882)the Korea Institute of Marine Science&Technology Promotion(KIMST),funded by the Ministry of Oceans and Fisheries,Korea(20220532).
文摘Methanol has recently gained significant attention as a potential carbon substrate for the production of fuels and chemicals,owing to its high degree of reduction,abundance,and low price.Native methylotrophic yeasts and bacteria have been investigated for the production of fuels and chemicals.Alternatively,synthetic methylotrophic strains are also being developed by reconstructing methanol utilization pathways in model microorganisms,such as Escherichia coli.Owing to the complex metabolic pathways,limited availability of genetic tools,and methanol/formaldehyde toxicity,the high-level production of target products for industrial applications are still under development to satisfy commercial feasibility.This article reviews the production of biofuels and chemicals by native and synthetic methylotrophic microorganisms.It also highlights the advantages and limitations of both types of methylotrophs and provides an overview of ways to improve their efficiency for the production of fuels and chemicals from methanol.
