Levitated optomechanical systems represent an excellent candidate platform for force and acceleration sensing.We propose a force-sensing protocol utilizing an optically levitated nanoparticle array.In our scheme,N nan...Levitated optomechanical systems represent an excellent candidate platform for force and acceleration sensing.We propose a force-sensing protocol utilizing an optically levitated nanoparticle array.In our scheme,N nanoparticles are trapped in an optical cavity using holographic optical tweezers.An external laser drives the cavity,exciting N cavity modes interacting simultaneously with the N nanoparticles.The optomechanical interaction encodes the information of the force acting on each nanoparticle onto the intracavity photons,which can be detected directly at the output ports of the cavity.Consequently,our protocol enables real-time imaging of a force field.展开更多
Pectobacterium carotovorum is the causal agent of bacterial soft rot in a wide range of vegetable host species.Once P.carotovorum infects the plant,the spread of the disease is difficult to control.In this study,a rap...Pectobacterium carotovorum is the causal agent of bacterial soft rot in a wide range of vegetable host species.Once P.carotovorum infects the plant,the spread of the disease is difficult to control.In this study,a rapid and sensitive method based on loop-mediated isothermal amplification(LAMP)was developed for detecting P.carotovorum in celery with soft rot using a primer set designed from the pmrA conserved sequence of P.carotovorum.The specificity of the LAMP primer set for P.carotovorum was extensively validated on both P.carotovorum strains and nontarget strains.The sensitivity was 1 pg of P.carotovorum genomic DNA,which demonstrated 10 times more sensitive than the conventional PCR assay.LAMP was also used to detect P.carotovorum in bacterial suspension.The lowest detection concentration was 104 CFU·mL^−1.In addition,a LAMP assay,in conjunction with a crude DNA extraction method,was successfully performed on P.carotovorum-infected samples derived from both artificially and naturally infected plants.In summary,the LAMP assay established in this study constitutes a simple,sensitive,and rapid method for the detection of P.carotovorum,and has potential application in the control of celery soft rot disease through early detection.展开更多
Nowadays,biohazard events caused by laboratory pathogen leaks and infectious disease transmission have become an increasingly important threat to human safety.The principal form of transmission of pathogenic microorga...Nowadays,biohazard events caused by laboratory pathogen leaks and infectious disease transmission have become an increasingly important threat to human safety.The principal form of transmission of pathogenic microorganisms is via aerosols,which could spread over a wide area within a short time.The system of biological aerosol recognition mainly involves early warning,field detection,as well as sampling and laboratory testing.Early warning about biological aerosols is essential for establishing a timely response.Once the presence of a biological aerosol is suspected,field detection is immediately performed to confirm the occurrence of the aerosol.Then,the aerosol is sampled using air sampling devices for laboratory identification of the microbial type.This review specifically intends to introduce the technologies and equipment for biological aerosol early warning and field detection based on laser-induced fluorescence(LIF).展开更多
We propose optical experiments to study the depth of field for a thermal light lensless ghost imaging system. It is proved that the diaphragm is an important factor to influence the depth of field, and the ghost image...We propose optical experiments to study the depth of field for a thermal light lensless ghost imaging system. It is proved that the diaphragm is an important factor to influence the depth of field, and the ghost images of two detected objects with longitudinal distance less than the depth of field can be achieved simultaneously. The longitudinal coherence scale of the thermal light lensless ghost imaging determines the depth of field. Theoretical analysis can well explain the experimental results.展开更多
Electromagnetic holographic data are characterized by two modes, suggesting that image reconstruction requires a dual-mode sensitivity field as well. We analyze an electromagnetic holographic field based on tomography...Electromagnetic holographic data are characterized by two modes, suggesting that image reconstruction requires a dual-mode sensitivity field as well. We analyze an electromagnetic holographic field based on tomography theory and Radon inverse transform to derive the expression of the electromagnetic holographic sensitivity field （EMHSF）. Then, we apply the EMHSF calculated by using finite-element methods to flow simulations and holographic imaging. The results suggest that the EMHSF based on the partial derivative of radius of the complex electric potential φ is closely linked to the Radon inverse transform and encompasses the sensitivities of the amplitude and phase data. The flow images obtained with inversion using EMHSF better agree with the actual flow patterns. The EMHSF overcomes the limitations of traditional single-mode sensitivity fields.展开更多
Wheat blast,caused by the fungus Magnaporthe oryzae Triticum(MoT)pathotype,is a devastating disease persistent in South America and Bangladesh.Since MoT generally fails to cause visual symptoms in wheat until the head...Wheat blast,caused by the fungus Magnaporthe oryzae Triticum(MoT)pathotype,is a devastating disease persistent in South America and Bangladesh.Since MoT generally fails to cause visual symptoms in wheat until the heading stage when the infection would have advanced,disease control by fungicide application solely based on the detection of visual symptoms is ineffective.To develop an accurate and sensitive method to detect MoT at the seedling and vegetative stages for disease control,we sequenced the genomes of two MoT isolates from Brazil and identified two DNA fragments,MoT-6098 and MoT-6099,that are present in the MoT genome but not in the genome of the rice-infecting Magnaporthe oryzae Oryzae(MoO)pathotype.Using polymerase chain reaction(PCR),we confirmed the specificity of the two markers in 53 MoT and MoO isolates from South America and Bangladesh.To test the efficiency of the two markers,we first established a loop-mediated isothermal amplification(LAMP)method to detect MoT at isothermal conditions,without the use of a PCR machine.Following this,we used the Cas12a protein and guide RNAs(gRNAs)to target the MoT-6098 and MoT-6099 sequences.The activated Cas12a showed indiscriminate single-stranded deoxyribonuclease(ssDNase)activity.We then combined targetdependent Cas12a ssDNase activation with recombinase polymerase amplification(RPA)and nucleic acid lateral flow immunoassay(NALFIA)to develop a method that accurately,sensitively,and cost-effectively detects MoT-specific DNA sequences in infected wheat plants.This novel technique can be easily adapted for the rapid detection of wheat blast and other important plant diseases in the field.展开更多
A new approach to detecting ocean eddies automatically from remote sensing imageries based on the ocean eddy＇s eigen-pattern in remote sensing imagery and ＂force field-based shape extracting method＂ is proposed. Fi...A new approach to detecting ocean eddies automatically from remote sensing imageries based on the ocean eddy＇s eigen-pattern in remote sensing imagery and ＂force field-based shape extracting method＂ is proposed. First, the analysis on extracting eddies＇ edges from remote sensing imagery using conventional edge detection arithmetic operators is performed and returns digitized vector edge data as a result. Second, attraction forces and fusion forces between edge curves were analyzed and calculated based on the vector eddy edges. Thirdly, the virtual significant spatial patterns of eddy were detected automatically using iterative repetition followed by optimized rule. Finally, the spatial form auto-detection of different types of ocean eddies was done using satellite images. The study verified that this is an effective way to identify and detect the ocean eddy with a complex form.展开更多
An integration processing system of three-dimensional laser scanning information visualization in goaf was developed. It is provided with multiple functions, such as laser scanning information management for goaf, clo...An integration processing system of three-dimensional laser scanning information visualization in goaf was developed. It is provided with multiple functions, such as laser scanning information management for goaf, cloud data de-noising optimization, construction, display and operation of three-dimensional model, model editing, profile generation, calculation of goaf volume and roof area, Boolean calculation among models and interaction with the third party soft ware. Concerning this system with a concise interface, plentiful data input/output interfaces, it is featured with high integration, simple and convenient operations of applications. According to practice, in addition to being well-adapted, this system is favorably reliable and stable.展开更多
Optically levitated nano-particle with spins is a promising system for high-precision measurement and quantum information processing. We theoretically analyze the ratio between the fluctuation of particle's displa...Optically levitated nano-particle with spins is a promising system for high-precision measurement and quantum information processing. We theoretically analyze the ratio between the fluctuation of particle's displacement caused by spins in magnetic field and caused by molecular collisions of the residual air. When the ratio is larger than unity, the displacement fluctuation of spins flipping can be remarkably detected. By theoretical analysis and numerical simulation, we propose and validate a scheme for the detection of gradient of the magnetic field by levitating ferromagnetic nano-particle, and also put forward a realizable detection scheme of the single spin by levitating nano-diamond particle with single nitrogen-vacancy(NV) centers.展开更多
基金the useful discussion.This work is supported by the Natural Science Foundation of Zhe-jiang Province(Grant No.LQ22A040010)the National Natural Science Foundation of China(Grant Nos.12304545 and 12204434).
文摘Levitated optomechanical systems represent an excellent candidate platform for force and acceleration sensing.We propose a force-sensing protocol utilizing an optically levitated nanoparticle array.In our scheme,N nanoparticles are trapped in an optical cavity using holographic optical tweezers.An external laser drives the cavity,exciting N cavity modes interacting simultaneously with the N nanoparticles.The optomechanical interaction encodes the information of the force acting on each nanoparticle onto the intracavity photons,which can be detected directly at the output ports of the cavity.Consequently,our protocol enables real-time imaging of a force field.
基金the earmarked fund for Beijing Innovation Consortium of Agriculture Research System(Grant No.BAIC-2019)Key Laboratory of Biology and Genetic Improvement of Horticultural Crops,Ministry of Agriculture,P.R.China,and Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences(Grant No.CAAS-ASTIPIVFCAAS).
文摘Pectobacterium carotovorum is the causal agent of bacterial soft rot in a wide range of vegetable host species.Once P.carotovorum infects the plant,the spread of the disease is difficult to control.In this study,a rapid and sensitive method based on loop-mediated isothermal amplification(LAMP)was developed for detecting P.carotovorum in celery with soft rot using a primer set designed from the pmrA conserved sequence of P.carotovorum.The specificity of the LAMP primer set for P.carotovorum was extensively validated on both P.carotovorum strains and nontarget strains.The sensitivity was 1 pg of P.carotovorum genomic DNA,which demonstrated 10 times more sensitive than the conventional PCR assay.LAMP was also used to detect P.carotovorum in bacterial suspension.The lowest detection concentration was 104 CFU·mL^−1.In addition,a LAMP assay,in conjunction with a crude DNA extraction method,was successfully performed on P.carotovorum-infected samples derived from both artificially and naturally infected plants.In summary,the LAMP assay established in this study constitutes a simple,sensitive,and rapid method for the detection of P.carotovorum,and has potential application in the control of celery soft rot disease through early detection.
文摘Nowadays,biohazard events caused by laboratory pathogen leaks and infectious disease transmission have become an increasingly important threat to human safety.The principal form of transmission of pathogenic microorganisms is via aerosols,which could spread over a wide area within a short time.The system of biological aerosol recognition mainly involves early warning,field detection,as well as sampling and laboratory testing.Early warning about biological aerosols is essential for establishing a timely response.Once the presence of a biological aerosol is suspected,field detection is immediately performed to confirm the occurrence of the aerosol.Then,the aerosol is sampled using air sampling devices for laboratory identification of the microbial type.This review specifically intends to introduce the technologies and equipment for biological aerosol early warning and field detection based on laser-induced fluorescence(LIF).
基金Supported by the Beijing Natural Science Foundation under Grant No 4133086the Fundamental Research Funds for th Central Universities under Grant No 2-9-2014-022
文摘We propose optical experiments to study the depth of field for a thermal light lensless ghost imaging system. It is proved that the diaphragm is an important factor to influence the depth of field, and the ghost images of two detected objects with longitudinal distance less than the depth of field can be achieved simultaneously. The longitudinal coherence scale of the thermal light lensless ghost imaging determines the depth of field. Theoretical analysis can well explain the experimental results.
基金supported by the National Science and Technology Major Project(No.2011ZX05020-006)
文摘Electromagnetic holographic data are characterized by two modes, suggesting that image reconstruction requires a dual-mode sensitivity field as well. We analyze an electromagnetic holographic field based on tomography theory and Radon inverse transform to derive the expression of the electromagnetic holographic sensitivity field （EMHSF）. Then, we apply the EMHSF calculated by using finite-element methods to flow simulations and holographic imaging. The results suggest that the EMHSF based on the partial derivative of radius of the complex electric potential φ is closely linked to the Radon inverse transform and encompasses the sensitivities of the amplitude and phase data. The flow images obtained with inversion using EMHSF better agree with the actual flow patterns. The EMHSF overcomes the limitations of traditional single-mode sensitivity fields.
基金The data that support the findings of this study have being submitted to GenBank and the accession numbers are JAAXMV000000000 and JAAXMU000000000.
文摘Wheat blast,caused by the fungus Magnaporthe oryzae Triticum(MoT)pathotype,is a devastating disease persistent in South America and Bangladesh.Since MoT generally fails to cause visual symptoms in wheat until the heading stage when the infection would have advanced,disease control by fungicide application solely based on the detection of visual symptoms is ineffective.To develop an accurate and sensitive method to detect MoT at the seedling and vegetative stages for disease control,we sequenced the genomes of two MoT isolates from Brazil and identified two DNA fragments,MoT-6098 and MoT-6099,that are present in the MoT genome but not in the genome of the rice-infecting Magnaporthe oryzae Oryzae(MoO)pathotype.Using polymerase chain reaction(PCR),we confirmed the specificity of the two markers in 53 MoT and MoO isolates from South America and Bangladesh.To test the efficiency of the two markers,we first established a loop-mediated isothermal amplification(LAMP)method to detect MoT at isothermal conditions,without the use of a PCR machine.Following this,we used the Cas12a protein and guide RNAs(gRNAs)to target the MoT-6098 and MoT-6099 sequences.The activated Cas12a showed indiscriminate single-stranded deoxyribonuclease(ssDNase)activity.We then combined targetdependent Cas12a ssDNase activation with recombinase polymerase amplification(RPA)and nucleic acid lateral flow immunoassay(NALFIA)to develop a method that accurately,sensitively,and cost-effectively detects MoT-specific DNA sequences in infected wheat plants.This novel technique can be easily adapted for the rapid detection of wheat blast and other important plant diseases in the field.
文摘A new approach to detecting ocean eddies automatically from remote sensing imageries based on the ocean eddy＇s eigen-pattern in remote sensing imagery and ＂force field-based shape extracting method＂ is proposed. First, the analysis on extracting eddies＇ edges from remote sensing imagery using conventional edge detection arithmetic operators is performed and returns digitized vector edge data as a result. Second, attraction forces and fusion forces between edge curves were analyzed and calculated based on the vector eddy edges. Thirdly, the virtual significant spatial patterns of eddy were detected automatically using iterative repetition followed by optimized rule. Finally, the spatial form auto-detection of different types of ocean eddies was done using satellite images. The study verified that this is an effective way to identify and detect the ocean eddy with a complex form.
基金Project(51274250)supported by the National Natural Science Foundation of ChinaProject(2012BAK09B02-05)supported by the National Key Technology R&D Program during the 12th Five-year Plan of China
文摘An integration processing system of three-dimensional laser scanning information visualization in goaf was developed. It is provided with multiple functions, such as laser scanning information management for goaf, cloud data de-noising optimization, construction, display and operation of three-dimensional model, model editing, profile generation, calculation of goaf volume and roof area, Boolean calculation among models and interaction with the third party soft ware. Concerning this system with a concise interface, plentiful data input/output interfaces, it is featured with high integration, simple and convenient operations of applications. According to practice, in addition to being well-adapted, this system is favorably reliable and stable.
基金Supported by the National Natural Science Foundation of China under Grant Nos.11374032,61435007,and 11704026the Joint Fund of the Ministry of Education of China under Grant No.6141A02011604+1 种基金NSAF(China)under Grant No.U1530401National Key Research and Development Program of China under Grant No.2016YFA0301201
文摘Optically levitated nano-particle with spins is a promising system for high-precision measurement and quantum information processing. We theoretically analyze the ratio between the fluctuation of particle's displacement caused by spins in magnetic field and caused by molecular collisions of the residual air. When the ratio is larger than unity, the displacement fluctuation of spins flipping can be remarkably detected. By theoretical analysis and numerical simulation, we propose and validate a scheme for the detection of gradient of the magnetic field by levitating ferromagnetic nano-particle, and also put forward a realizable detection scheme of the single spin by levitating nano-diamond particle with single nitrogen-vacancy(NV) centers.
