The Mediterranean Sea is home to an interesting biodiversity. The current cumulative and descriptive study aims to enumerate some relatively large marine mammals, reptiles, and fishes that have been spotted, caught, b...The Mediterranean Sea is home to an interesting biodiversity. The current cumulative and descriptive study aims to enumerate some relatively large marine mammals, reptiles, and fishes that have been spotted, caught, by-caught, or stranded on the coast of the Gaza Strip, Palestine, which extends about 42 km along the Mediterranean Sea. This 20-year study from 2003 to 2022 relied much on frequent field visits, meetings, and discussions with stakeholders, following local media sites and social media pages, and photography. The study recorded at least 26 giants or relatively large marine organisms on the Mediterranean coast of the Gaza Strip, Palestine. Certainly, the coming years may bring other marine organisms of relatively large sizes. Marine mammals included three species of cetaceans with the Fin Whale (Balaenoptera physalus) being the largest mammal and even the largest animal ever recorded in this study. Marine reptiles included three species of sea turtles, the largest of which is the Leatherback Sea Turtle (Dermochelys coriacea), which is in fact the world’s largest sea turtle. The bony fishes were represented by ten species, the largest of which was the Ocean Sunfish (Mola mola), which is the largest bony fish in the world. The cartilaginous fishes included ten species with the largest specimens encountered were the Shortfin Shark (Isurus oxyrinchus), Bluntnose Sixgill Shark (Hexanchus griseus), Scalloped Hammerhead Shark (Sphyrna lewini), and Giant Devil Ray (Mobula mobular). Seabirds were not included in the study. The Alexandria Pompano (Alectis alexandrinus) and the Silver-Cheeked Toadfish (Lagocephalus sceleratus), which are bony fishes, appear to be the relatively smallest marine organisms recorded here. In conclusion, the role of the various parties must be coordinated to ensure the sustainability of human activities and their compatibility with the task of conserving local marine biota, including the gigantic or relatively large ones.展开更多
Our study was carried out to determine the phenotypic characterization antimicrobial susceptibility of Proteus mirabilis from fish in FCT, Abuja using isolation, selective plating, preliminary observation, complete bi...Our study was carried out to determine the phenotypic characterization antimicrobial susceptibility of Proteus mirabilis from fish in FCT, Abuja using isolation, selective plating, preliminary observation, complete biochemical method and antimicrobial susceptibility testing. The biochemical tests conducted includes include Citrate Utilization test, Triple Sugar Iron test, Urea test, Methyl Red test, Indole test, and Voges Proskauer test. The isolates were confirmed by Microbact<sup>TM</sup>GNB24E identification kit (Oxiod, UK). A total of 400 fish samples were bought in the market from three area council of the FCT. The result of the study showed overall prevalence rate of (13) 3.25% of Proteus mirabilis isolates. Distribution based on Area councils showed that AMAC had higher prevalence rate of 4.81%, while Bwari had 2.99% and Gwagwalada with 2.57% prevalence. All isolates were subjected to antimicrobial susceptibility testing using the modified single disc diffusion method. From the antimicrobial susceptibility testing done it was discovered that Proteus mirabilis are resistant to Amoxyclav (100%), Erythromycin (92.3%), Tetracycline (92.3%) and Ceftriaxone (23.1%). However, the isolates were susceptible to Ofloxacin (100%), Netillin (92.3%), Levofloxacin (92.3%), Ceftazidime (76.9%), Co-trimoxazole (69.2%) and Gentamicin (61.5%). Since Proteus mirabilis sources of zoonotic diseases and can potentially be dangerous to humans and other animals, our research was able to isolate it from fresh water fish sold in the Federal Capital Territory. This makes public health awareness of the risks associated with Proteus mirabilis in Nigeria necessary.展开更多
Accurate species identification is a key component of biodiversity research.DNA barcoding is an effective molecular method used for fish species identification.We aimed to study the DNA barcoding of fish in Zhoushan c...Accurate species identification is a key component of biodiversity research.DNA barcoding is an effective molecular method used for fish species identification.We aimed to study the DNA barcoding of fish in Zhoushan coastal waters,explore the differences and applicability of two gene fragments(12S rRNA and COI)of DNA barcoding in fish species identification,and established a comprehensive fish barcoding reference database.Two hundred and eighty-seven captured fish samples from Zhoushan coastal waters were identified using morphological characteristics and DNA barcoding.A total of 26412S rRNA sequences(belonging to eight orders,31 families,55 genera,and 66 species)and 188 COI sequences(belonging to seven orders,30 families,48 genera,and 58 species)were obtained.The lengths of the 12S rRNA sequences ranged from 165 to 178 bp,and the guanine-cytosine(GC)content was 45.37%.The average 12S rRNA interspecific and intraspecific genetic distances(K2P)were 0.10%and 26.66%,respectively.The length of the COI sequence ranged 574–655 bp,and the content of GC was 45.97%.The average 12S rRNA interspecific and intraspecific genetic distances(K2P)were 0.16%and 27.45%,respectively.The minimum interspecific genetic distances of 12S rRNA and COI(1.23%and 1.86%)were both greater than their maximum intraspecific genetic distances(2.42%and 8.66%).Three molecular analyses(NJ tree,ABGD,and GMYC)were performed to accurately identify and delineate species.Clustering errors occurred when the 12S rRNA sequences were delimited using the NJ tree method,and the delimitation results of ABGD and GMYC are consistent with the final species identification results.Our results demonstrate that DNA barcoding based on 12S rRNA and COI can be used as an effective tool for fish species identification,and 12S rRNA has good application prospects in the environmental DNA(eDNA)metabarcoding of marine fish.展开更多
Phylogeny of the sinipercine fishes, their relationships with some fossils and their systematic position were studied in this paper. Consisting of 2 sister groups, the sinipercine fishes were grouped as 2 genera - Cor...Phylogeny of the sinipercine fishes, their relationships with some fossils and their systematic position were studied in this paper. Consisting of 2 sister groups, the sinipercine fishes were grouped as 2 genera - Coreoperca and Siniperca, while the genus Coreosiniperca was unwarranted for its type being a member in Siniperca, Having many special characters, Tungtingichthys was denied as the ancestor of the sinipercine fishes, however, Siniperca wusiangensis was taken as one member in Siniperca and some fossil percoids found in Japan had close relationship with sinipercine fishes. All characters of the sinipercine fishes are not concordant with that in Serranidae or Percichthyidae which leads to the conclusion that either the sinipercine fishes are not in Percichthyidae, or the defining characters of Percichthyidae listed by Gosline (1966) are wrong. But at present, we can only put the sinipercine fishes in Percichthyidae.展开更多
According to an ancient belief from Turkish, Greek, Roman, and many Europe countries, an auspicious eye can bring fortunesto the people, protect them from evil.
The hypertrophic subtropic Donghu Lake’s dense water bloom(of mainly Microcystis Anaabaena andOscillatoria)that occurred annually from the beginning of the 1970s, has disappeared since 1985. The infiuenceof planktivo...The hypertrophic subtropic Donghu Lake’s dense water bloom(of mainly Microcystis Anaabaena andOscillatoria)that occurred annually from the beginning of the 1970s, has disappeared since 1985. The infiuenceof planktivorous fishes (silver and bighead carps) on the water bloom was studied for three years usingthe enclosure method. The enclosures stocked densely with bighead and/or silver carp were free of waterbloom during the experimental period. The water bloom that appeared in the fish-free enclosures was completelyeliminated in 10-20 days by introduction of silver and/or bighead carp(grass carp was not effectivein controlling water bloom).This study showed clearly that grazing pressure by planktivorous fishes is a keyfactor in eliminating water bloom from the lake.展开更多
AIM To study the cell types,localization,distribution density and morphology of APUDcells in the intestinal mucosa of stomachlessteleost fishes.METHOD By using the peroxidase-antiperoxidase complex(PAP)immunocytochemi...AIM To study the cell types,localization,distribution density and morphology of APUDcells in the intestinal mucosa of stomachlessteleost fishes.METHOD By using the peroxidase-antiperoxidase complex(PAP)immunocytochemical staining technique theidentification,localization and morphology ofimmunoreactive(IR)endocrine cells seattered inthe intestinal mucosa of grass carp(Cyenopharyngodon idellus),black carp(Mylopharyngodon piceus)and common carp(Cyprinus carpio)were investigated with 20kinds of antisera prepared against mammalianpeptide hormones of APUD cells,and likewise byusing avidin-biotin-peroxidase complex(ABC)method those of silver carp(Hypophthalmichthys molitrix),bighead(Aristichthys nobilis),silver crucian carp(Carassius gibelio)and bluntnose black bream(Megalobrama amblyocephala)were alsostudied with 5 different antisera.Thereplacement of the first antiserum by phosphatebuffered saline(PBS)was employed as a control.IR endocrine cells were counted with asquare-mesh ocular micrometer from 10 fieldsselected randomly in every section of each partof the intestine specimen.The average numberof IR endocrine cells per mm2 was counted toquantify their distribution density.RESULT Gastrin(GAS)-,Gastric inhibitorypeptide(GIP)-,glucagon(GLU)-,glucagon-likeimmunoreactants(GLI)-,bovine pancreaticpolypeptide(BPP)-,leucine-enkephalin(ENK)-and substance P(SP)-IR endocrine cells werefound in the gut of grass carp,black carp andcommon carp,and somatostatin(SOM)-IRendocrine cells were only seen in common carp.GAS-,GIP-and GLU-IR endocrine cells werefound in the intestinal mucosa of silver carp,bighead,silver crucian carp and bluntnose blackbream.Most of IR endocrine cells had the higherdistribution density in the foregut and midgut,and were longer in shape.They had a long apicalcytoplasmic process extended to the gut lumenand a basal process extended to adjacent cellsor basement membrane and touched with it.Sometimes,the basal cytoplasmic processformed an enlarged synapse-like structure in thecontiguous part with basement membrane.Thisphenomenon provided new morphologicalevidence for neuroendocrine and paracrinesecretory function of these enteroendocrinecells.CONCLUTION At least 8 kinds of IR endocrinecells were found in the gut of stomachlessteleost species for the first time in China.TheseIR endocrine cells scattering in the gut mucosabelong to the APUD system.Among them,thehormones secreted by SP-,ENK-,SOM-and GLU-IR endocrine cells belong to the peptides of dualdistribution in the brain and gut.This providednew evidence for the concept of brain-gutpeptide.According to the cell types,distribution density,morphologicalcharacteristics and variety in shape of APUDcells in the gut of stomachless teleost fishes,itis deemed that the digestive tract of fishes isalso an endocrine organ of great importance andcomplexity.展开更多
Spatial distribution and abundance of small fishes were studied in autumn 2007 in the Xiaosihai Lake, a shallow lake along the middle reach of the Changjiang （Yangtze） River. Based on the plant cover, the lake was d...Spatial distribution and abundance of small fishes were studied in autumn 2007 in the Xiaosihai Lake, a shallow lake along the middle reach of the Changjiang （Yangtze） River. Based on the plant cover, the lake was divided into three major habitats： Myriophyllum spicaturn habitat （MS habitat）, Trapa bispinosa habitat （TB habitat）, and non-vegetation habitat （NV habitat）. A modified pop-net was used for quantitative sampling of small fishes in the three habitats, and the Zippin＇s removal method was used for estimating densities of the small fishes. A total of 13 species belonging to 5 families were collected, with 11 species in MS habitat, 7 species in TB habitat, and 5 species in NV habitat. Habitat type had significant effect on the spatial distribution of small fishes. The Shannon-Wiener diversity index in the MS, TB and NV habitats were 1.28, 0.56 and 0.54, respectively. The total density and biomass of small fishes were significantly higher in the MS habitat （13.68 ind/m^2 and 4.44 g/m^2） than in the TB habitat （1.41 ind/m^2 and 0.54 g/m^2） and the NV habitat （1.08 ind/m^2 and 0.40 g/m^2）, and were not significantly different between the TB habitat and the NV habitat. Water depth had no significant effect on spatial distribution of the small fishes. It was suggested that vegetation type played an important role in habitat selectivity of small fishes, and the presence of submersed vegetation should be of significance in the conservation of small fish diversity.展开更多
Compound-specific stable isotope analysis of individual amino acids(CSIA-AA)has been widely used in ecological and biogeochemical studies.It has been proven to be powerful in tracing the diet sources and trophic inter...Compound-specific stable isotope analysis of individual amino acids(CSIA-AA)has been widely used in ecological and biogeochemical studies.It has been proven to be powerful in tracing the diet sources and trophic interactions.However,assessing the N sources of mesopelagic fishes has been inconclusive because the mesopelagic fishes’unique domain(water depth ranged from 0 to 1000 m)and unresolved nitrogen isotopes of various forms.This study proposes a new method for coupling instruments(ion chromatography and PreconIRMS)and chemical method of oxidation-reduction of amino acids,and also combinedδ15N of AAs withδ13C of fatty acids(FAs)to analyze the trophic interactions of mesopelagic fishes in the South China Sea(SCS).AAs were isolated by ion chromatography with high peak resolution and collected by an automated fraction collector.The chemical method then converted the AAs into N2 O with a robust oxidation yields and suitable molar ratio of NH2 OH to.Finally,theδ15N of AAs at 20 nmol were measured with a reasonable precision(<0.6‰).With this method,this study report the first batch high precisionδ15N of AAs andδ13C of FAs of mesopelagic fishes collected from SCS.Diaphus luetkeni,Chauliodus minimus and Bathygadus antrodes showed similarδ13C values of 20:4 n-6(~-28‰),while Argyropelecus affinis and Stomias had similar values(~-32‰).These results reflect that mesopelagic fishes had complex diet sources.An increase of 4‰inδ15N of glutamic acid(Glu)was found between piscivorous and planktivorous fishes,which might suggest a trophic discrimination factor of mesopelagic fishes in the SCS.This study usedδ13C of 20:4 n-6 to reveal the diet sources of mesopelagic fishes andδ15N of Glu to clarify trophic level between piscivorous and planktivorous fishes.Thus,this combinative method could therefore ultimately be applied in a variety of deep-sea ecosystem.展开更多
Heavy metal concentrations of Ni, Mg, Co, Cr, Cd and Cu were investigatedusing flame Atomic Absorption Spectrophotometer (AAS) in the liver, kidney, muscle, scale, gonad, gill and stomach of Cheilinus trilobatus fish ...Heavy metal concentrations of Ni, Mg, Co, Cr, Cd and Cu were investigatedusing flame Atomic Absorption Spectrophotometer (AAS) in the liver, kidney, muscle, scale, gonad, gill and stomach of Cheilinus trilobatus fish species from the northern Gulf of Aqaba. Cheilinus trilobatus fish species were collected during the period May to August 2013. The results indicated significant differences between different organs of Cheilinus trilobatus for Ni (p = 0.0002), Fe (p = 0.0213), Co (p p Cheilinus trilobatus and its length. The levels of metal concentration of the present study were generally lower or within the ranges of those found in the fish of the Red Sea. After all, Cheilinus trilobatus fish species was found to be safe for consumption and do not pose a significant threat to the health of human consumers.展开更多
Analysis of stomach contents of samples of clupeid fiishes (Japanese anchovy, half-fin anchovy, zunas’ scaled sardine, rednosed glassnose and tapertail anchovy) collected from the catches of a 1982-1984 fishery resou...Analysis of stomach contents of samples of clupeid fiishes (Japanese anchovy, half-fin anchovy, zunas’ scaled sardine, rednosed glassnose and tapertail anchovy) collected from the catches of a 1982-1984 fishery resource survey showed they are zooplanktonivorous. Similar food groups, such as copepoda, Chaetognatha, Amphipoda and Mysidacea were found in the stomach. Dietary similarity coefficients were studied to evaluate the degree of similarity in the dietary species-pairs.Sea-sonal variation is discussed.展开更多
The knowledge of prey small ?sh stock, distribution and abundance is necessary to guide stocking of piscivorous ?sh for the biomanipulation in domestic tap water lakes. This study describes the current status of small...The knowledge of prey small ?sh stock, distribution and abundance is necessary to guide stocking of piscivorous ?sh for the biomanipulation in domestic tap water lakes. This study describes the current status of small ?sh community in Kuilei Lake(China), and examines the spatial and seasonal variations of the community in relation to key environmental factors. Based on submerged macrophyte cover and water depth, the lake was divided into ?ve major habitats:(1) macrophyte covered shallow habitat of water depth< 2.00 m,(2) uncovered or less-covered shallow habitat(2.00 m–3.50 m),(3) uncovered medium shallow habitat(3.50 m–5.00 m),(4) uncovered medium deep habitat(5.00 m–6.50 m) and(5) uncovered deep habitat(6.50 m–8.50 m). The abundance and composition of small ?sh were monitored by benthic fykenet sampling from April 2013 to January 2014. A total of 2881 individuals belonging to 5 families and 21 species were collected. Based on their abundance(accounted for 88.96% of the total) and occurrence(more than 33.33%), Acheilognathus chankaensis, Acheilognathus macropterus, Microphysogobio microstomus,Pseudorasbora parva and Rhinogobius giurinus were recognized as dominant small ?sh species. The results of correlation analysis identi?ed that species richness( Sr), Shannon-Wiener diversity index( H′)and Margalef′s richness index( D) were signi?cantly negatively correlated with water depth, but positively correlated with biomass of submerged macrophytes.Redundancy analysis(RDA) revealed that the spatial distributions of most small ?shes were negatively associated with water depth. The details of these ?ndings are bene?cial to understanding the adaptation of the small ?shes in degraded environments, and to developing suitable biomanipulation strategies for the management of ?sh resources and water quality in the lakes along the lower reach of the Changjiang(Yangtze) River basin.展开更多
A fuzzy mathematical method is used to discriminate the similarities of pelagic fishes land- ed in the 9180 hauls by 10 light-seine information vessels in the southern Fujian waters from 1989 to 1998. The results indi...A fuzzy mathematical method is used to discriminate the similarities of pelagic fishes land- ed in the 9180 hauls by 10 light-seine information vessels in the southern Fujian waters from 1989 to 1998. The results indicate that the dominant species of the communities had an obvious alternation and the fuzzy adjacency of annual species composition varied between 0. 659 and 0. 923 with an average value of 0. 791. The fuzzy clustering analysis indicates that the annual fuzzy adjacency in general remains good although the species composition of the pelagic fishes has changed to a certain degree since 1992. This paper concludes that the community structure of pelagic fishes in the southern Taiwan Strait remains rel- atively stable and the state of fish stocks shows a good potentiality for a larger utilization.展开更多
This article first analyzes the status quo of industrial development of cold water fishes in Henan Province. And then it points out the main problems in the process of cold water fish industrialization as follows: the...This article first analyzes the status quo of industrial development of cold water fishes in Henan Province. And then it points out the main problems in the process of cold water fish industrialization as follows: the parent fish rearing of cold water fishes and the supply of roe and fry are inadequate; the demand for feed nutrition,research and development of disease prevention and control technology lag behind. In order to provide scientific basis for promoting the industrial independent innovation and international competitiveness,finally the following ideas and recommendations for industrial development of cold water fishes are put forward: strengthening policy support for the enterprises,and striving to create the leading enterprises,to achieve dramatic development; implementing the strategy of developing fishery by relying on science and technology,promoting the industrial independent innovation and competitiveness; creating specialized cooperative organizations,actively improving the supporting system,and expanding the space for development of the industry.展开更多
Invasion, spread and predominance of alien invasive fishes were studied in 950-km-long stretch of the Yamuna River. The fish yield ranged between 32 t/km/yr to 240 t/km/yr and the catch of alien invasive fishes domina...Invasion, spread and predominance of alien invasive fishes were studied in 950-km-long stretch of the Yamuna River. The fish yield ranged between 32 t/km/yr to 240 t/km/yr and the catch of alien invasive fishes dominated over local fishes in the fishery, which was over 96% at Etawah. The calculated Invasion Coefficient Index (Ixi) of alien invasive fishes was in the range of 0.12 to 0.31 indicating a major loss to the local fish diversity. The calculated Pearson’s correlation coefficient was found to have positive correlation of 0.757 and 0.710 for common carp and tilapia respectively with locally captured Indian major carps (IMC). The results of this study highlighted how unintentional spread of alien invasive fishes due to rapid aquaculture diversification and intensification has invaded into the Yamuna River causing severe loss to the local fishery. The invasion of alien fishes has been mapped for the Yamuna River at different locations using GIS tools. The results of this study invite attention on the management and sustainability of the local fishery and ecosystem health of the river.展开更多
A total of 32 parasite species were documented from 658 clariid fishes examined. Thirteen species were nematodes, eleven digeneans, three cestodes, two crustaceans, and the Monogenea, Acanthocephala and Hirudinea were...A total of 32 parasite species were documented from 658 clariid fishes examined. Thirteen species were nematodes, eleven digeneans, three cestodes, two crustaceans, and the Monogenea, Acanthocephala and Hirudinea were each represented by one species. Of the parasites recovered 16 were identified to species level, 12 to genus and 4 parasites were not identified. Most of the parasites recorded in the present study have been reported in fish ofTanzaniaand elsewhere, however, there were a few first records, and these included Neogoezia sp., Comephoronema sp., Travnema sp., Quimperia sp. and Gendria tilapiae. It is stipulated that these parasites represent the original fauna of the lake prior to introduction of the alien species. The clariid fish species investigated were a good model for studying the parasites of fish in the lake because of having a variety of parasites some of which being specific to certain clariid fishes, for instance Diplostomum and Tylodelphys species infecting only C. gariepinus and the camallanids being typical of the clariid fishes.展开更多
Objective The Early Cretaceous sediments are well-exposed in southern Jilin Province,and yield abundant invertebrate and plant fossils,including the typical EosestheriaEphemeropsis trisetalis-Lycoptera（E-E-L）assembl...Objective The Early Cretaceous sediments are well-exposed in southern Jilin Province,and yield abundant invertebrate and plant fossils,including the typical EosestheriaEphemeropsis trisetalis-Lycoptera（E-E-L）assemblage of the Jehol Biota（Shao Tiequan et al.,2017）.However,vertebrate fossils,especially tetrapods,are extremely rare and there is no formal documents on these fossils.Recently,a new fossil site dominated by fishes and turtles has been discovered in the middle part of the展开更多
In this current paper, the exposure time effects on four endocrine disruptors and teleost fishes were evaluated using the reduced life expectancy (RLE) model based on the effect concentration (EC<sub>50</sub&...In this current paper, the exposure time effects on four endocrine disruptors and teleost fishes were evaluated using the reduced life expectancy (RLE) model based on the effect concentration (EC<sub>50</sub>) of available literature published. The result on the regression analysis over different exposure times has demonstrated that the EC<sub>50</sub> of hepatic biomarkers falls with increasing exposure times in a predictable manner. The slopes of the regression equations reflect the strength of the toxic effects on the various teleost fish. The EC<sub>50</sub> reduction over time can be interpreted based on the bioconcentration process, which can be used to understand transfer routes of the compounds from water to fish body. RLE model also provides useful information in assessing the toxic effects on fish life expectancy as a result of the occurrence of compounds.展开更多
As a summary of the finding from the investigation of fishes in Beitang Estuary,Tianjin,the paper deals with the young fishes 'biomass, composition and change, and studies the spawning habit, spawning time, fecund...As a summary of the finding from the investigation of fishes in Beitang Estuary,Tianjin,the paper deals with the young fishes 'biomass, composition and change, and studies the spawning habit, spawning time, fecundity, age structure and type of the spawning stocks in the estuary.展开更多
The malate(EC 1.1.1.37)and lactate(EC 1.1.1.27)dehydrogenases are themetabolic enzymes directly or indirectly involved in energy production,gluconeogenesis and lipogenesis.Malate dehydrogenase(MDH)exists in twoisoenzy...The malate(EC 1.1.1.37)and lactate(EC 1.1.1.27)dehydrogenases are themetabolic enzymes directly or indirectly involved in energy production,gluconeogenesis and lipogenesis.Malate dehydrogenase(MDH)exists in twoisoenzymic forms,cytoplasmic(cMDH)and mitochondrial(mMDH),composed of Aand/or B subunits(dimeric molecule:MW 40,000-120,000).Lactate dehydrogenase(LDH)has tetrameric(MW 35,000-110,000)structure made up of either A and/or B,orC(C,E,F)subunits.They catalyze an ordered bisubstrate(substrate and coenzyme)展开更多
文摘The Mediterranean Sea is home to an interesting biodiversity. The current cumulative and descriptive study aims to enumerate some relatively large marine mammals, reptiles, and fishes that have been spotted, caught, by-caught, or stranded on the coast of the Gaza Strip, Palestine, which extends about 42 km along the Mediterranean Sea. This 20-year study from 2003 to 2022 relied much on frequent field visits, meetings, and discussions with stakeholders, following local media sites and social media pages, and photography. The study recorded at least 26 giants or relatively large marine organisms on the Mediterranean coast of the Gaza Strip, Palestine. Certainly, the coming years may bring other marine organisms of relatively large sizes. Marine mammals included three species of cetaceans with the Fin Whale (Balaenoptera physalus) being the largest mammal and even the largest animal ever recorded in this study. Marine reptiles included three species of sea turtles, the largest of which is the Leatherback Sea Turtle (Dermochelys coriacea), which is in fact the world’s largest sea turtle. The bony fishes were represented by ten species, the largest of which was the Ocean Sunfish (Mola mola), which is the largest bony fish in the world. The cartilaginous fishes included ten species with the largest specimens encountered were the Shortfin Shark (Isurus oxyrinchus), Bluntnose Sixgill Shark (Hexanchus griseus), Scalloped Hammerhead Shark (Sphyrna lewini), and Giant Devil Ray (Mobula mobular). Seabirds were not included in the study. The Alexandria Pompano (Alectis alexandrinus) and the Silver-Cheeked Toadfish (Lagocephalus sceleratus), which are bony fishes, appear to be the relatively smallest marine organisms recorded here. In conclusion, the role of the various parties must be coordinated to ensure the sustainability of human activities and their compatibility with the task of conserving local marine biota, including the gigantic or relatively large ones.
文摘Our study was carried out to determine the phenotypic characterization antimicrobial susceptibility of Proteus mirabilis from fish in FCT, Abuja using isolation, selective plating, preliminary observation, complete biochemical method and antimicrobial susceptibility testing. The biochemical tests conducted includes include Citrate Utilization test, Triple Sugar Iron test, Urea test, Methyl Red test, Indole test, and Voges Proskauer test. The isolates were confirmed by Microbact<sup>TM</sup>GNB24E identification kit (Oxiod, UK). A total of 400 fish samples were bought in the market from three area council of the FCT. The result of the study showed overall prevalence rate of (13) 3.25% of Proteus mirabilis isolates. Distribution based on Area councils showed that AMAC had higher prevalence rate of 4.81%, while Bwari had 2.99% and Gwagwalada with 2.57% prevalence. All isolates were subjected to antimicrobial susceptibility testing using the modified single disc diffusion method. From the antimicrobial susceptibility testing done it was discovered that Proteus mirabilis are resistant to Amoxyclav (100%), Erythromycin (92.3%), Tetracycline (92.3%) and Ceftriaxone (23.1%). However, the isolates were susceptible to Ofloxacin (100%), Netillin (92.3%), Levofloxacin (92.3%), Ceftazidime (76.9%), Co-trimoxazole (69.2%) and Gentamicin (61.5%). Since Proteus mirabilis sources of zoonotic diseases and can potentially be dangerous to humans and other animals, our research was able to isolate it from fresh water fish sold in the Federal Capital Territory. This makes public health awareness of the risks associated with Proteus mirabilis in Nigeria necessary.
基金Supported by the Zhejiang Provincial Key Research and Development Program (No.2021C02047)。
文摘Accurate species identification is a key component of biodiversity research.DNA barcoding is an effective molecular method used for fish species identification.We aimed to study the DNA barcoding of fish in Zhoushan coastal waters,explore the differences and applicability of two gene fragments(12S rRNA and COI)of DNA barcoding in fish species identification,and established a comprehensive fish barcoding reference database.Two hundred and eighty-seven captured fish samples from Zhoushan coastal waters were identified using morphological characteristics and DNA barcoding.A total of 26412S rRNA sequences(belonging to eight orders,31 families,55 genera,and 66 species)and 188 COI sequences(belonging to seven orders,30 families,48 genera,and 58 species)were obtained.The lengths of the 12S rRNA sequences ranged from 165 to 178 bp,and the guanine-cytosine(GC)content was 45.37%.The average 12S rRNA interspecific and intraspecific genetic distances(K2P)were 0.10%and 26.66%,respectively.The length of the COI sequence ranged 574–655 bp,and the content of GC was 45.97%.The average 12S rRNA interspecific and intraspecific genetic distances(K2P)were 0.16%and 27.45%,respectively.The minimum interspecific genetic distances of 12S rRNA and COI(1.23%and 1.86%)were both greater than their maximum intraspecific genetic distances(2.42%and 8.66%).Three molecular analyses(NJ tree,ABGD,and GMYC)were performed to accurately identify and delineate species.Clustering errors occurred when the 12S rRNA sequences were delimited using the NJ tree method,and the delimitation results of ABGD and GMYC are consistent with the final species identification results.Our results demonstrate that DNA barcoding based on 12S rRNA and COI can be used as an effective tool for fish species identification,and 12S rRNA has good application prospects in the environmental DNA(eDNA)metabarcoding of marine fish.
文摘Phylogeny of the sinipercine fishes, their relationships with some fossils and their systematic position were studied in this paper. Consisting of 2 sister groups, the sinipercine fishes were grouped as 2 genera - Coreoperca and Siniperca, while the genus Coreosiniperca was unwarranted for its type being a member in Siniperca, Having many special characters, Tungtingichthys was denied as the ancestor of the sinipercine fishes, however, Siniperca wusiangensis was taken as one member in Siniperca and some fossil percoids found in Japan had close relationship with sinipercine fishes. All characters of the sinipercine fishes are not concordant with that in Serranidae or Percichthyidae which leads to the conclusion that either the sinipercine fishes are not in Percichthyidae, or the defining characters of Percichthyidae listed by Gosline (1966) are wrong. But at present, we can only put the sinipercine fishes in Percichthyidae.
文摘According to an ancient belief from Turkish, Greek, Roman, and many Europe countries, an auspicious eye can bring fortunesto the people, protect them from evil.
基金This work was supported by FEBL(State Key Laboratory for Freshwater Ecology and Biotechnology of China)fundsNational Natural Science Foundation of China (Grant NO.3937014)
文摘The hypertrophic subtropic Donghu Lake’s dense water bloom(of mainly Microcystis Anaabaena andOscillatoria)that occurred annually from the beginning of the 1970s, has disappeared since 1985. The infiuenceof planktivorous fishes (silver and bighead carps) on the water bloom was studied for three years usingthe enclosure method. The enclosures stocked densely with bighead and/or silver carp were free of waterbloom during the experimental period. The water bloom that appeared in the fish-free enclosures was completelyeliminated in 10-20 days by introduction of silver and/or bighead carp(grass carp was not effectivein controlling water bloom).This study showed clearly that grazing pressure by planktivorous fishes is a keyfactor in eliminating water bloom from the lake.
基金the National Natural Science Foundation of China.No.39070666.
文摘AIM To study the cell types,localization,distribution density and morphology of APUDcells in the intestinal mucosa of stomachlessteleost fishes.METHOD By using the peroxidase-antiperoxidase complex(PAP)immunocytochemical staining technique theidentification,localization and morphology ofimmunoreactive(IR)endocrine cells seattered inthe intestinal mucosa of grass carp(Cyenopharyngodon idellus),black carp(Mylopharyngodon piceus)and common carp(Cyprinus carpio)were investigated with 20kinds of antisera prepared against mammalianpeptide hormones of APUD cells,and likewise byusing avidin-biotin-peroxidase complex(ABC)method those of silver carp(Hypophthalmichthys molitrix),bighead(Aristichthys nobilis),silver crucian carp(Carassius gibelio)and bluntnose black bream(Megalobrama amblyocephala)were alsostudied with 5 different antisera.Thereplacement of the first antiserum by phosphatebuffered saline(PBS)was employed as a control.IR endocrine cells were counted with asquare-mesh ocular micrometer from 10 fieldsselected randomly in every section of each partof the intestine specimen.The average numberof IR endocrine cells per mm2 was counted toquantify their distribution density.RESULT Gastrin(GAS)-,Gastric inhibitorypeptide(GIP)-,glucagon(GLU)-,glucagon-likeimmunoreactants(GLI)-,bovine pancreaticpolypeptide(BPP)-,leucine-enkephalin(ENK)-and substance P(SP)-IR endocrine cells werefound in the gut of grass carp,black carp andcommon carp,and somatostatin(SOM)-IRendocrine cells were only seen in common carp.GAS-,GIP-and GLU-IR endocrine cells werefound in the intestinal mucosa of silver carp,bighead,silver crucian carp and bluntnose blackbream.Most of IR endocrine cells had the higherdistribution density in the foregut and midgut,and were longer in shape.They had a long apicalcytoplasmic process extended to the gut lumenand a basal process extended to adjacent cellsor basement membrane and touched with it.Sometimes,the basal cytoplasmic processformed an enlarged synapse-like structure in thecontiguous part with basement membrane.Thisphenomenon provided new morphologicalevidence for neuroendocrine and paracrinesecretory function of these enteroendocrinecells.CONCLUTION At least 8 kinds of IR endocrinecells were found in the gut of stomachlessteleost species for the first time in China.TheseIR endocrine cells scattering in the gut mucosabelong to the APUD system.Among them,thehormones secreted by SP-,ENK-,SOM-and GLU-IR endocrine cells belong to the peptides of dualdistribution in the brain and gut.This providednew evidence for the concept of brain-gutpeptide.According to the cell types,distribution density,morphologicalcharacteristics and variety in shape of APUDcells in the gut of stomachless teleost fishes,itis deemed that the digestive tract of fishes isalso an endocrine organ of great importance andcomplexity.
基金Supported by the National Natural Science Foundation of China (Nos. 30571440, 30830025)The National Key Technology R&D Program (No. 2007BAD37B03)the National Basic Research Program of China (973 Program) (No. 2007CB109205)
文摘Spatial distribution and abundance of small fishes were studied in autumn 2007 in the Xiaosihai Lake, a shallow lake along the middle reach of the Changjiang （Yangtze） River. Based on the plant cover, the lake was divided into three major habitats： Myriophyllum spicaturn habitat （MS habitat）, Trapa bispinosa habitat （TB habitat）, and non-vegetation habitat （NV habitat）. A modified pop-net was used for quantitative sampling of small fishes in the three habitats, and the Zippin＇s removal method was used for estimating densities of the small fishes. A total of 13 species belonging to 5 families were collected, with 11 species in MS habitat, 7 species in TB habitat, and 5 species in NV habitat. Habitat type had significant effect on the spatial distribution of small fishes. The Shannon-Wiener diversity index in the MS, TB and NV habitats were 1.28, 0.56 and 0.54, respectively. The total density and biomass of small fishes were significantly higher in the MS habitat （13.68 ind/m^2 and 4.44 g/m^2） than in the TB habitat （1.41 ind/m^2 and 0.54 g/m^2） and the NV habitat （1.08 ind/m^2 and 0.40 g/m^2）, and were not significantly different between the TB habitat and the NV habitat. Water depth had no significant effect on spatial distribution of the small fishes. It was suggested that vegetation type played an important role in habitat selectivity of small fishes, and the presence of submersed vegetation should be of significance in the conservation of small fish diversity.
基金The National Basic Research Program(973 Program)of China under contract No.2014CB441502the National Natural Science Foundation of China under contract No.41876074the Cross-research Center Project by QNLM under contract No.JCZX202007。
文摘Compound-specific stable isotope analysis of individual amino acids(CSIA-AA)has been widely used in ecological and biogeochemical studies.It has been proven to be powerful in tracing the diet sources and trophic interactions.However,assessing the N sources of mesopelagic fishes has been inconclusive because the mesopelagic fishes’unique domain(water depth ranged from 0 to 1000 m)and unresolved nitrogen isotopes of various forms.This study proposes a new method for coupling instruments(ion chromatography and PreconIRMS)and chemical method of oxidation-reduction of amino acids,and also combinedδ15N of AAs withδ13C of fatty acids(FAs)to analyze the trophic interactions of mesopelagic fishes in the South China Sea(SCS).AAs were isolated by ion chromatography with high peak resolution and collected by an automated fraction collector.The chemical method then converted the AAs into N2 O with a robust oxidation yields and suitable molar ratio of NH2 OH to.Finally,theδ15N of AAs at 20 nmol were measured with a reasonable precision(<0.6‰).With this method,this study report the first batch high precisionδ15N of AAs andδ13C of FAs of mesopelagic fishes collected from SCS.Diaphus luetkeni,Chauliodus minimus and Bathygadus antrodes showed similarδ13C values of 20:4 n-6(~-28‰),while Argyropelecus affinis and Stomias had similar values(~-32‰).These results reflect that mesopelagic fishes had complex diet sources.An increase of 4‰inδ15N of glutamic acid(Glu)was found between piscivorous and planktivorous fishes,which might suggest a trophic discrimination factor of mesopelagic fishes in the SCS.This study usedδ13C of 20:4 n-6 to reveal the diet sources of mesopelagic fishes andδ15N of Glu to clarify trophic level between piscivorous and planktivorous fishes.Thus,this combinative method could therefore ultimately be applied in a variety of deep-sea ecosystem.
文摘Heavy metal concentrations of Ni, Mg, Co, Cr, Cd and Cu were investigatedusing flame Atomic Absorption Spectrophotometer (AAS) in the liver, kidney, muscle, scale, gonad, gill and stomach of Cheilinus trilobatus fish species from the northern Gulf of Aqaba. Cheilinus trilobatus fish species were collected during the period May to August 2013. The results indicated significant differences between different organs of Cheilinus trilobatus for Ni (p = 0.0002), Fe (p = 0.0213), Co (p p Cheilinus trilobatus and its length. The levels of metal concentration of the present study were generally lower or within the ranges of those found in the fish of the Red Sea. After all, Cheilinus trilobatus fish species was found to be safe for consumption and do not pose a significant threat to the health of human consumers.
文摘Analysis of stomach contents of samples of clupeid fiishes (Japanese anchovy, half-fin anchovy, zunas’ scaled sardine, rednosed glassnose and tapertail anchovy) collected from the catches of a 1982-1984 fishery resource survey showed they are zooplanktonivorous. Similar food groups, such as copepoda, Chaetognatha, Amphipoda and Mysidacea were found in the stomach. Dietary similarity coefficients were studied to evaluate the degree of similarity in the dietary species-pairs.Sea-sonal variation is discussed.
基金Supported by the National Science and Technology Supporting(No.2012BAD25B08)the Special Fund for Agro-scientific Research in the Public Interest(No.201303056)the Earmarked Fund for China Agriculture Research System(No.CARS-45)
文摘The knowledge of prey small ?sh stock, distribution and abundance is necessary to guide stocking of piscivorous ?sh for the biomanipulation in domestic tap water lakes. This study describes the current status of small ?sh community in Kuilei Lake(China), and examines the spatial and seasonal variations of the community in relation to key environmental factors. Based on submerged macrophyte cover and water depth, the lake was divided into ?ve major habitats:(1) macrophyte covered shallow habitat of water depth< 2.00 m,(2) uncovered or less-covered shallow habitat(2.00 m–3.50 m),(3) uncovered medium shallow habitat(3.50 m–5.00 m),(4) uncovered medium deep habitat(5.00 m–6.50 m) and(5) uncovered deep habitat(6.50 m–8.50 m). The abundance and composition of small ?sh were monitored by benthic fykenet sampling from April 2013 to January 2014. A total of 2881 individuals belonging to 5 families and 21 species were collected. Based on their abundance(accounted for 88.96% of the total) and occurrence(more than 33.33%), Acheilognathus chankaensis, Acheilognathus macropterus, Microphysogobio microstomus,Pseudorasbora parva and Rhinogobius giurinus were recognized as dominant small ?sh species. The results of correlation analysis identi?ed that species richness( Sr), Shannon-Wiener diversity index( H′)and Margalef′s richness index( D) were signi?cantly negatively correlated with water depth, but positively correlated with biomass of submerged macrophytes.Redundancy analysis(RDA) revealed that the spatial distributions of most small ?shes were negatively associated with water depth. The details of these ?ndings are bene?cial to understanding the adaptation of the small ?shes in degraded environments, and to developing suitable biomanipulation strategies for the management of ?sh resources and water quality in the lakes along the lower reach of the Changjiang(Yangtze) River basin.
基金This project is funded by the Fujian Department of Fisheries (Min Shui Ke 1998-08).
文摘A fuzzy mathematical method is used to discriminate the similarities of pelagic fishes land- ed in the 9180 hauls by 10 light-seine information vessels in the southern Fujian waters from 1989 to 1998. The results indicate that the dominant species of the communities had an obvious alternation and the fuzzy adjacency of annual species composition varied between 0. 659 and 0. 923 with an average value of 0. 791. The fuzzy clustering analysis indicates that the annual fuzzy adjacency in general remains good although the species composition of the pelagic fishes has changed to a certain degree since 1992. This paper concludes that the community structure of pelagic fishes in the southern Taiwan Strait remains rel- atively stable and the state of fish stocks shows a good potentiality for a larger utilization.
基金Supported by Special Fund for Agro-scientific Research in the Public Interest ( 201003055)
文摘This article first analyzes the status quo of industrial development of cold water fishes in Henan Province. And then it points out the main problems in the process of cold water fish industrialization as follows: the parent fish rearing of cold water fishes and the supply of roe and fry are inadequate; the demand for feed nutrition,research and development of disease prevention and control technology lag behind. In order to provide scientific basis for promoting the industrial independent innovation and international competitiveness,finally the following ideas and recommendations for industrial development of cold water fishes are put forward: strengthening policy support for the enterprises,and striving to create the leading enterprises,to achieve dramatic development; implementing the strategy of developing fishery by relying on science and technology,promoting the industrial independent innovation and competitiveness; creating specialized cooperative organizations,actively improving the supporting system,and expanding the space for development of the industry.
文摘Invasion, spread and predominance of alien invasive fishes were studied in 950-km-long stretch of the Yamuna River. The fish yield ranged between 32 t/km/yr to 240 t/km/yr and the catch of alien invasive fishes dominated over local fishes in the fishery, which was over 96% at Etawah. The calculated Invasion Coefficient Index (Ixi) of alien invasive fishes was in the range of 0.12 to 0.31 indicating a major loss to the local fish diversity. The calculated Pearson’s correlation coefficient was found to have positive correlation of 0.757 and 0.710 for common carp and tilapia respectively with locally captured Indian major carps (IMC). The results of this study highlighted how unintentional spread of alien invasive fishes due to rapid aquaculture diversification and intensification has invaded into the Yamuna River causing severe loss to the local fishery. The invasion of alien fishes has been mapped for the Yamuna River at different locations using GIS tools. The results of this study invite attention on the management and sustainability of the local fishery and ecosystem health of the river.
文摘A total of 32 parasite species were documented from 658 clariid fishes examined. Thirteen species were nematodes, eleven digeneans, three cestodes, two crustaceans, and the Monogenea, Acanthocephala and Hirudinea were each represented by one species. Of the parasites recovered 16 were identified to species level, 12 to genus and 4 parasites were not identified. Most of the parasites recorded in the present study have been reported in fish ofTanzaniaand elsewhere, however, there were a few first records, and these included Neogoezia sp., Comephoronema sp., Travnema sp., Quimperia sp. and Gendria tilapiae. It is stipulated that these parasites represent the original fauna of the lake prior to introduction of the alien species. The clariid fish species investigated were a good model for studying the parasites of fish in the lake because of having a variety of parasites some of which being specific to certain clariid fishes, for instance Diplostomum and Tylodelphys species infecting only C. gariepinus and the camallanids being typical of the clariid fishes.
基金supported by the National Natural Science Foundation of China(grants No.41202012 and 41172009)China Geological Survey(grant No.1212011120149)+1 种基金the Project ‘‘111’’,China(grant No.B-06008)Shandong University of Science&Technology Research Fund(grant No.2015TDJH101)
文摘Objective The Early Cretaceous sediments are well-exposed in southern Jilin Province,and yield abundant invertebrate and plant fossils,including the typical EosestheriaEphemeropsis trisetalis-Lycoptera（E-E-L）assemblage of the Jehol Biota（Shao Tiequan et al.,2017）.However,vertebrate fossils,especially tetrapods,are extremely rare and there is no formal documents on these fossils.Recently,a new fossil site dominated by fishes and turtles has been discovered in the middle part of the
文摘In this current paper, the exposure time effects on four endocrine disruptors and teleost fishes were evaluated using the reduced life expectancy (RLE) model based on the effect concentration (EC<sub>50</sub>) of available literature published. The result on the regression analysis over different exposure times has demonstrated that the EC<sub>50</sub> of hepatic biomarkers falls with increasing exposure times in a predictable manner. The slopes of the regression equations reflect the strength of the toxic effects on the various teleost fish. The EC<sub>50</sub> reduction over time can be interpreted based on the bioconcentration process, which can be used to understand transfer routes of the compounds from water to fish body. RLE model also provides useful information in assessing the toxic effects on fish life expectancy as a result of the occurrence of compounds.
文摘As a summary of the finding from the investigation of fishes in Beitang Estuary,Tianjin,the paper deals with the young fishes 'biomass, composition and change, and studies the spawning habit, spawning time, fecundity, age structure and type of the spawning stocks in the estuary.
文摘The malate(EC 1.1.1.37)and lactate(EC 1.1.1.27)dehydrogenases are themetabolic enzymes directly or indirectly involved in energy production,gluconeogenesis and lipogenesis.Malate dehydrogenase(MDH)exists in twoisoenzymic forms,cytoplasmic(cMDH)and mitochondrial(mMDH),composed of Aand/or B subunits(dimeric molecule:MW 40,000-120,000).Lactate dehydrogenase(LDH)has tetrameric(MW 35,000-110,000)structure made up of either A and/or B,orC(C,E,F)subunits.They catalyze an ordered bisubstrate(substrate and coenzyme)
