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转录因子ATF3与前列腺癌临床相关性的分析研究
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作者 徐凌凡 丁和康 +2 位作者 施浩强 杨诚 邰胜 《安徽医专学报》 2024年第2期90-93,共4页
目的:探究转录因子ATF3与前列腺癌的临床相关性,了解ATF3在前列腺癌组织中的表达以及其表达强度对前列腺癌患者预后的影响。方法:采用公共数据库挖掘的方法分析ATF3在前列腺癌组织中的突变情况以及ATF3在正常前列腺组织以及前列腺癌组... 目的:探究转录因子ATF3与前列腺癌的临床相关性,了解ATF3在前列腺癌组织中的表达以及其表达强度对前列腺癌患者预后的影响。方法:采用公共数据库挖掘的方法分析ATF3在前列腺癌组织中的突变情况以及ATF3在正常前列腺组织以及前列腺癌组织中的表达情况,kaplan-meier方法分析ATF3的表达强度与前列腺癌患者临床预后的相关性;免疫组化染色验证ATF3在正常前列腺组织和前列腺癌组织中的蛋白表达;患者临床信息分析ATF3的表达高低与前列腺癌临床特征的关联。结果:ATF3在前列腺癌组织中表现为高扩增突变,且ATF3在前列腺癌组织中的表达明显高于正常前列腺组织;ATF3与前列腺癌疾病的发生、进展(病灶转移)相关;高表达ATF3的前列腺癌患者预后较差。结论:ATF3的表达强度与前列腺癌的发生发展相关,可作为前列腺癌诊断及预后判断的重要指标。 展开更多
关键词 前列腺癌 atf3 免疫组化 前列腺特异性抗原
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黑逍遥散调控p38MAPK/ATF2/COX-2信号通路对阿尔茨海默病大鼠神经炎症的影响 被引量:3
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作者 米彩云 彭超 +2 位作者 周君 李明成 王虎平 《中成药》 CAS CSCD 北大核心 2024年第2期590-595,共6页
目的 观察黑逍遥散对Aβ_(1-42)所致阿尔茨海默病(AD)大鼠学习记忆能力的影响,并从p38MAPK/ATF2/COX-2信号通路介导的炎症级联反应探讨其作用机制。方法 双侧海马注射1μL Aβ_(1-42)溶液复刻AD大鼠模型。筛选造模成功的大鼠50只,随机... 目的 观察黑逍遥散对Aβ_(1-42)所致阿尔茨海默病(AD)大鼠学习记忆能力的影响,并从p38MAPK/ATF2/COX-2信号通路介导的炎症级联反应探讨其作用机制。方法 双侧海马注射1μL Aβ_(1-42)溶液复刻AD大鼠模型。筛选造模成功的大鼠50只,随机分为模型组、盐酸多奈哌齐组(0.5 mg/kg)和黑逍遥散低、中、高剂量组(4.25、8.5、17 g/kg),连续给药42 d。Morris水迷宫实验检测定位航行与空间探索能力,HE染色观察海马神经元病理结构改变,ELISA法检测海马组织Aβ_(1-42)、iNOS、PGE_(2)表达,RT-qPCR法检测海马组织p38、ATF2、COX-2 mRNA表达,Western blot法检测海马组织p-p38、p-ATF2、COX-2蛋白表达。结果 与模型组比较,黑逍遥散中、高剂量组和盐酸多奈哌齐组逃避潜伏期缩短(P<0.01),有效区域运动距离、目标象限滞留时间百分比增加(P<0.01),海马CA1区神经元排列有序,胞体清晰,凋亡细胞减少,海马组织Aβ_(1-42)、iNOS、PGE_(2)水平降低(P<0.05,P<0.01),p38、COX-2 mRNA表达降低(P<0.05,P<0.01),p38、p-p38、p-ATF2、COX-2蛋白表达降低(P<0.01)。结论 黑逍遥散可改善Aβ_(1-42)所致AD大鼠的认知能力,其机制可能与阻断p38MAPK/ATF2/COX-2信号通路传导,进而减轻炎症反应有关。 展开更多
关键词 黑逍遥散 阿尔茨海默病 神经炎症 p38MAPK/atf2/COX-2信号通路
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雷公藤红素通过调控ATF4/caspase-3/GSDME信号通路促进肝癌细胞焦亡 被引量:2
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作者 李端 王永辉 +2 位作者 周静 张乐 孔永红 《中国药理学通报》 CAS CSCD 北大核心 2024年第2期299-307,共9页
目的探究雷公藤红素(tripterine,TRI)抑制肝癌细胞(hepatocellular carcinoma,HCC)进展的新机制。方法利用不同浓度(0、0.5、2和8μmol·L-1)TRI作用于肝癌细胞,然后利用CCK-8、细胞克隆、Transwell和蛋白免疫印迹等实验方法评估细... 目的探究雷公藤红素(tripterine,TRI)抑制肝癌细胞(hepatocellular carcinoma,HCC)进展的新机制。方法利用不同浓度(0、0.5、2和8μmol·L-1)TRI作用于肝癌细胞,然后利用CCK-8、细胞克隆、Transwell和蛋白免疫印迹等实验方法评估细胞表型和可能机制;再利用siRNA将靶基因GSDME进行干扰,确定GSDME的作用;最后使用移植肿瘤模型验证TRI在体内抑制HCC细胞的生长的机制。结果TRI可抑制HCC细胞增殖、克隆和侵袭,促进细胞焦亡。免疫印迹结果显示,TRI处理后肝细胞癌HepG2或Hepa1-6中GSDME表达均上调,同时cleaved caspase-3和PARP也明显升高。敲除GSDME后可部分逆转TRI诱导的细胞焦亡。同时GSDME敲低后的细胞具有更强的克隆和迁移能力,且与单独的TRI治疗组相比,细胞凋亡率降低。在体内实验中,TRI抑制可HCC肿瘤生长,TRI+siGSDME组小鼠肿瘤生长速度比单独TRI治疗组快。此外,TRI刺激后HepG2和Hepa1-6细胞中p-eIF2a、ATF4均升高。免疫荧光结果显示,TRI刺激后HepG2和Hepa1-6细胞中ATF4阳性细胞数呈剂量依赖性增加。结论TRI抑制肝癌细胞生长和侵袭可能与其调控ATF4/caspase-3/GSDME信号通路促进肝癌细胞焦亡有关。 展开更多
关键词 肝癌细胞 细胞焦亡 雷公藤红素 GSDME atf4 CASPASE3
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基于PERK/ATF4/CHOP信号通路研究滋阴明目方含药血清对衣霉素诱导的ARPE-19细胞的作用机制 被引量:1
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作者 谢薇 彭俊 +2 位作者 宋厚盼 欧晨 彭清华 《湖南中医药大学学报》 CAS 2024年第5期785-790,共6页
目的研究滋阴明目方含药血清对衣霉素诱导ARPE-19细胞的影响及其可能机制。方法构建细胞内质网应激损伤模型,将ARPE-19细胞分为空白组、模型组、空白血清组、滋阴明目方含药血清组、牛磺熊去氧胆酸组。对细胞进行形态观察,CCK-8检测细... 目的研究滋阴明目方含药血清对衣霉素诱导ARPE-19细胞的影响及其可能机制。方法构建细胞内质网应激损伤模型,将ARPE-19细胞分为空白组、模型组、空白血清组、滋阴明目方含药血清组、牛磺熊去氧胆酸组。对细胞进行形态观察,CCK-8检测细胞存活率,TUNEL法检测细胞凋亡,Western blot检测细胞蛋白激酶样内质网激酶(PERK)、活化转录因子4(ATF4)、C/EBP同源蛋白(CHOP)蛋白的表达。结果选用浓度50μmol/L衣霉素干预ARPE-19细胞造模。观察细胞形态发现,滋阴明目方含药血清组和牛磺熊去氧胆酸组ARPE-19细胞较模型组细胞数量增多,生长较均匀,漂浮的死亡ARPE-19细胞及碎片减少。与空白组相比,模型组和空白血清组的细胞存活率下降(P<0.01),凋亡率明显上升(P<0.01),PERK、ATF4、CHOP蛋白表达上调(P<0.01)。与模型组相比,滋阴明目方含药血清组细胞存活率上升(P<0.01),凋亡率明显下降(P<0.01),PERK、ATF4、CHOP蛋白表达下调(P<0.01)。结论滋阴明目方含药血清可以减少ARPE-19细胞内质网应激损伤模型的凋亡,其分子机制与调控PERK-ATF4-CHOP信号通路有关。 展开更多
关键词 滋阴明目方 ARPE-19细胞 衣霉素 内质网应激损伤模型 PERK/atf4/CHOP信号通路
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葛根芩连汤对2型糖尿病db/db小鼠胰腺组织PERK/ATF4/CHOP信号通路的影响 被引量:1
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作者 关晓文 梁永林 +3 位作者 朱向东 苏菲 张媛媛 翟艳会 《中国中医药信息杂志》 CAS CSCD 2024年第1期97-103,共7页
目的观察葛根芩连汤对2型糖尿病(T2DM)小鼠胰腺内质网应激的影响,探讨其治疗T2DM的作用机制。方法75只SPF级雄性db/db小鼠随机分为模型组、二甲双胍组和葛根芩连汤高、中、低剂量组,每组15只,15只db/m小鼠作为空白组,给药组分别予相应... 目的观察葛根芩连汤对2型糖尿病(T2DM)小鼠胰腺内质网应激的影响,探讨其治疗T2DM的作用机制。方法75只SPF级雄性db/db小鼠随机分为模型组、二甲双胍组和葛根芩连汤高、中、低剂量组,每组15只,15只db/m小鼠作为空白组,给药组分别予相应药物灌胃12周。检测小鼠体质量、空腹血糖(FBG)及糖化血红蛋白(HbA1c),HE染色观察胰腺组织病理变化,TUNEL染色检测胰岛细胞凋亡情况,Western blot检测胰腺组织葡萄糖调节蛋白78(GRP78)、蛋白激酶R样内质网激酶(PERK)、p-PERK、活化转录因子4(ATF4)、C/EBP同源蛋白(CHOP)蛋白表达,实时荧光定量PCR检测胰腺组织PERK、ATF4、CHOP mRNA表达。结果与空白组比较,模型组小鼠体质量、FBG和HbA1c含量显著增加(P<0.01);胰腺组织结构不完整,边界模糊,内有空泡,胰岛细胞凋亡明显增加(P<0.01);胰腺组织GRP78、p-PERK、ATF4、CHOP蛋白表达显著升高(P<0.01),PERK、ATF4、CHOP mRNA表达显著升高(P<0.01)。与模型组比较,各给药组小鼠体质量、FBG和HbA1c含量显著减少(P<0.05,P<0.01);胰腺组织病理改变有所减轻,胰岛细胞凋亡不同程度减少(P<0.05,P<0.01);葛根芩连汤高、中剂量组和二甲双胍组胰腺组织GRP78、p-PERK、ATF4、CHOP蛋白表达显著降低(P<0.01),PERK、ATF4、CHOP mRNA表达显著降低(P<0.05,P<0.01)。结论葛根芩连汤可能通过抑制内质网应激PERK/ATF4/CHOP信号通路,下调相关基因和蛋白表达,减少胰岛细胞凋亡,保护胰岛细胞功能,延缓T2DM进展。 展开更多
关键词 葛根芩连汤 2型糖尿病 内质网应激 PERK/atf4/CHOP信号通路 小鼠
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FABP4沉默通过调节PERK/eIF2α/ATF4/CHOP信号通路对妊娠糖尿病大鼠内质网应激和胰岛素抵抗的影响 被引量:1
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作者 李贞 余冬平 +3 位作者 罗武 陶婷 陈辉 王宁 《中国免疫学杂志》 CAS CSCD 北大核心 2024年第4期705-713,共9页
目的:探讨脂肪酸结合蛋白4(FABP4)沉默通过调节蛋白激酶R样内质网激酶(PERK)/真核细胞启动因子2α(eIF2α)/转录因子4(ATF4)/C/EBP同源蛋白(CHOP)信号通路对妊娠糖尿病(GDM)大鼠内质网应激(ERS)和胰岛素抵抗(IR)的影响。方法:腹腔注射... 目的:探讨脂肪酸结合蛋白4(FABP4)沉默通过调节蛋白激酶R样内质网激酶(PERK)/真核细胞启动因子2α(eIF2α)/转录因子4(ATF4)/C/EBP同源蛋白(CHOP)信号通路对妊娠糖尿病(GDM)大鼠内质网应激(ERS)和胰岛素抵抗(IR)的影响。方法:腹腔注射链脲佐菌素(STZ)建立GDM大鼠模型。通过尾静脉注射FABP4 siRNA质粒(si-FABP4)、阴性对照质粒(NC)和PERK激活剂(CCT020312),将大鼠随机分为Normal组、GDM组、GDM+NC组、GDM+si-FABP4组、GDM+si-FABP4+CCT020312组。检测胰腺组织中FABP4含量、血脂水平、炎症标志物水平和氧化应激标志物水平,检测胰腺组织中PERK/eIF2α/ATF4/CHOP信号通路相关蛋白表达。HTR-8/SVneo细胞分为5组:对照(Control)组、高糖(HG)组、HG+NC组、HG+si-FABP4组、HG+si-FABP4+CCT020312组,24 h后检测细胞中FABP4及PERK/eIF2α/ATF4/CHOP信号通路相关蛋白表达。结果:与Normal组相比,GDM组大鼠血清和胰腺组织中FABP4水平显著上调(P<0.05)。FABP4沉默显著降低了FBG和IR,并伴有血脂、CRP、TNF-α、IL-6及MDA水平降低和SOD、CAT水平升高(P<0.05)。此外,FABP4沉默减轻了胰腺和胎盘组织损伤。而CCT020312上调PERK、eIF2α磷酸化水平和ATF4、CHOP蛋白表达后,FABP4沉默对GDM大鼠IR、炎症、氧化应激以及胰腺和胎盘损伤的抑制作用均被逆转(P<0.05)。FABP4在HG诱导的HTR-8/SVneo细胞中上调表达,沉默FABP4可抑制PERK/eIF2α/ATF4/CHOP通路蛋白表达,CCT020312则逆转这种变化(P<0.05)。结论:FABP4沉默通过抑制炎症和氧化应激,改善GDM大鼠的IR和ERS,其机制与抑制PERK/eIF2α/ATF4/CHOP信号通路有关。 展开更多
关键词 FABP4 PERK/eIF2α/atf4/CHOP信号通路 妊娠糖尿病 内质网应激 胰岛素抵抗
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BATF2在胃癌组织中的表达及与患者临床病理特征和预后的关系
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作者 郭明海 刘记恩 +3 位作者 刘小慧 张云杰 王伟栋 李明辉 《胃肠病学和肝病学杂志》 CAS 2024年第8期982-986,共5页
目的 探讨碱性亮氨酸拉链转录因子2(basic leucine zipper ATF-like transcription factor 2,BATF2)在胃癌组织中的表达及与患者临床病理特征和预后的关系。方法 收集我院2016年6月至2017年11月134例胃癌患者手术切除的胃癌组织及癌旁... 目的 探讨碱性亮氨酸拉链转录因子2(basic leucine zipper ATF-like transcription factor 2,BATF2)在胃癌组织中的表达及与患者临床病理特征和预后的关系。方法 收集我院2016年6月至2017年11月134例胃癌患者手术切除的胃癌组织及癌旁组织标本,采用qRT-PCR法测定BATF2的mRNA表达,免疫组化法检测BATF2蛋白表达。分析胃癌患者BATF2表达与临床病理特征间的关系。采用Cox回归分析胃癌患者预后影响因素,Kaplan-Meier法分析BATF2表达与患者预后的关系。结果 胃癌组织中BATF2 mRNA和蛋白表达均显著低于癌旁组织(P<0.05),淋巴结转移、TNM分期为Ⅲ+Ⅳ期、细胞浸润程度为T_(3)~T_(4)、细胞分化程度为低分化的胃癌患者BATF2蛋白阳性表达比例显著低于淋巴结未转移、TNM分期为Ⅰ+Ⅱ期、细胞浸润程度为T_(0)~T_(2)、细胞分化程度为中/高分化的患者(P<0.05)。多因素Cox分析结果表明,淋巴结转移、TNM分期、细胞分化程度、BATF2是胃癌患者预后、复发或死亡的影响因素(P<0.05);Kaplan-Meier法分析结果表明,胃癌患者5年内预后良好率为74.63%(100/134),BATF2高表达患者5年预后良好率(93.33%)显著高于BATF2低表达患者(69.23%)(χ~2=6.445,P<0.05)。结论 BATF2表达与胃癌患者临床病理特征具有密切关系,是胃癌患者复发或死亡的影响因素,BATF2表达上调可能提高胃癌患者生存率。 展开更多
关键词 碱性亮氨酸拉链转录因子2 胃癌 临床病理特征 预后
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Effect of fracture fluid flowback on shale microfractures using CT scanning 被引量:2
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作者 Jiale He Zhihong Zhao +6 位作者 Yiran Geng Yuping Chen Jianchun Guo Cong Lu Shouyi Wang Xueliang Han Jun Zhang 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第2期426-436,共11页
The field data of shale fracturing demonstrate that the flowback performance of fracturing fluid is different from that of conventional reservoirs,where the flowback rate of shale fracturing fluid is lower than that o... The field data of shale fracturing demonstrate that the flowback performance of fracturing fluid is different from that of conventional reservoirs,where the flowback rate of shale fracturing fluid is lower than that of conventional reservoirs.At the early stage of flowback,there is no single-phase flow of the liquid phase in shale,but rather a gas-water two-phase flow,such that the single-phase flow model for tight oil and gas reservoirs is not applicable.In this study,pores and microfractures are extracted based on the experimental results of computed tomography(CT)scanning,and a spatial model of microfractures is established.Then,the influence of rough microfracture surfaces on the flow is corrected using the modified cubic law,which was modified by introducing the average deviation of the microfracture height as a roughness factor to consider the influence of microfracture surface roughness.The flow in the fracture network is simulated using the modified cubic law and the lattice Boltzmann method(LBM).The results obtained demonstrate that most of the fracturing fluid is retained in the shale microfractures,which explains the low fracturing fluid flowback rate in shale hydraulic fracturing. 展开更多
关键词 SHALE Flowback of fracturing fluid MICROFRACTURE Lattice Boltzmann method(LBM)
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Synthetic polymers:A review of applications in drilling fluids 被引量:2
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作者 Shadfar Davoodi Mohammed Al-Shargabi +2 位作者 David A.Wood Valeriy S.Rukavishnikov Konstantin M.Minaev 《Petroleum Science》 SCIE EI CAS CSCD 2024年第1期475-518,共44页
With the growth of deep drilling and the complexity of the well profile,the requirements for a more complete and efficient exploitation of productive formations increase,which increases the risk of various complicatio... With the growth of deep drilling and the complexity of the well profile,the requirements for a more complete and efficient exploitation of productive formations increase,which increases the risk of various complications.Currently,reagents based on modified natural polymers(which are naturally occurring compounds)and synthetic polymers(SPs)which are polymeric compounds created industrially,are widely used to prevent emerging complications in the drilling process.However,compared to modified natural polymers,SPs form a family of high-molecular-weight compounds that are fully synthesized by undergoing chemical polymerization reactions.SPs provide substantial flexibility in their design.Moreover,their size and chemical composition can be adjusted to provide properties for nearly all the functional objectives of drilling fluids.They can be classified based on chemical ingredients,type of reaction,and their responses to heating.However,some of SPs,due to their structural characteristics,have a high cost,a poor temperature and salt resistance in drilling fluids,and degradation begins when the temperature reaches 130℃.These drawbacks prevent SP use in some medium and deep wells.Thus,this review addresses the historical development,the characteristics,manufacturing methods,classification,and the applications of SPs in drilling fluids.The contributions of SPs as additives to drilling fluids to enhance rheology,filtrate generation,carrying of cuttings,fluid lubricity,and clay/shale stability are explained in detail.The mechanisms,impacts,and advances achieved when SPs are added to drilling fluids are also described.The typical challenges encountered by SPs when deployed in drilling fluids and their advantages and drawbacks are also discussed.Economic issues also impact the applications of SPs in drilling fluids.Consequently,the cost of the most relevant SPs,and the monomers used in their synthesis,are assessed.Environmental impacts of SPs when deployed in drilling fluids,and their manufacturing processes are identified,together with advances in SP-treatment methods aimed at reducing those impacts.Recommendations for required future research addressing SP property and performance gaps are provided. 展开更多
关键词 Synthetic versus natural polymers Nanopolymers Drilling fluid additives LUBRICITY Clay swelling Hole cleaning
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基于内质网应激PERK/ATF4/CHOP信号通路探讨丹参多酚酸盐对膜性肾病大鼠足细胞凋亡的影响
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作者 郭明鑫 高圣柠 +4 位作者 吴怡欢 毛竞宇 唐淼 吴红红 檀金川 《江苏中医药》 CAS 2024年第9期67-73,共7页
目的:基于蛋白激酶R样内质网激酶(PERK)/活化转录因子4(ATF4)/转录因子C/EBP同源蛋白(CHOP)信号通路探讨丹参多酚酸盐对膜性肾病(MN)大鼠内质网应激的影响。方法:将80只雄性SD大鼠随机分配,其中20只为正常组,剩余60只大鼠采用尾静脉注... 目的:基于蛋白激酶R样内质网激酶(PERK)/活化转录因子4(ATF4)/转录因子C/EBP同源蛋白(CHOP)信号通路探讨丹参多酚酸盐对膜性肾病(MN)大鼠内质网应激的影响。方法:将80只雄性SD大鼠随机分配,其中20只为正常组,剩余60只大鼠采用尾静脉注射阳离子化牛血清白蛋白(C-BSA)的方法诱导MN病变。将造模成功的大鼠随机分为模型组、贝那普利组和丹参多酚酸盐低、中、高剂量组,每组10只,另取10只正常组大鼠共同进行实验。贝那普利组每日给药剂量为10 mg/kg,丹参多酚酸盐低、中、高剂量组每日给药剂量分别为16.7、33.3、66.7 mg/kg,正常组和模型组予等体积生理盐水,各组均每日灌胃1次,连续4周。检测各组大鼠治疗后24 h尿蛋白定量(24 h-UTP);腹主动脉取血分离血清,检测各组大鼠血尿素氮(BUN)、肌酐(Scr)含量;过碘酸六胺银(PASM)染色后光镜观察各组大鼠肾组织病理形态,透射电子显微镜进一步观察各组大鼠肾组织细微结构变化;免疫荧光法检测各组大鼠肾组织免疫球蛋白G(IgG)沉积情况;免疫组化(IHC)法检测各组大鼠肾组织足细胞裂隙膜蛋白(Podocin)、足细胞裂孔隔膜蛋白(Nephrin)表达水平;蛋白免疫印迹(Western blot)法检测各组大鼠肾组织PERK、ATF4、CHOP、B细胞淋巴瘤-2(Bcl-2)、Bcl-2相关X蛋白(Bax)表达。结果:相较于正常组,模型组大鼠的肾组织显示出显著的病理性变化,肾小球基底膜显著增厚,并出现类似钉突的结构改变,同时伴有系膜基质的增生现象,沿毛细血管袢有IgG弥漫性沉积;各给药组大鼠肾组织上述病理改变有所缓解,足细胞损伤减少,IgG沉积减轻。模型组大鼠24 h-UTP水平显著高于正常组(P<0.01);各给药组大鼠24 h-UTP水平均显著低于模型组(P<0.05,P<0.01),其中丹参多酚酸盐中剂量组大鼠24 h-UTP水平与贝那普利组比较差异无统计学意义(P>0.05),丹参多酚酸盐高剂量组大鼠24 h-UTP水平明显低于贝那普利组(P<0.01),丹参多酚酸盐对MN模型大鼠24 h-UTP水平的降低作用具有明显的剂量依赖性(P<0.01)。各组大鼠血清BUN、Scr水平比较差异均无统计学意义(P>0.05)。模型组大鼠肾组织Podocin、Nephrin蛋白表达显著低于正常组(P<0.05);各给药组大鼠上述蛋白表达均显著高于模型组(P<0.05),其中丹参多酚酸盐中剂量组大鼠上述蛋白表达水平与贝那普利组比较差异无统计学意义(P>0.05),丹参多酚酸盐高剂量组大鼠上述蛋白表达水平明显高于贝那普利组(P<0.05),丹参多酚酸盐对MN模型大鼠上述蛋白表达水平的升高作用具有明显的剂量依赖性(P<0.05)。模型组大鼠肾组织PERK、ATF4、CHOP、Bax蛋白表达水平均明显高于正常组(P<0.01),Bcl-2蛋白表达水平明显低于正常组(P<0.01);各给药组大鼠肾组织上述蛋白表达水平均较模型组显著改善(P<0.05,P<0.01),其中丹参多酚酸盐中剂量组大鼠上述蛋白表达水平与贝那普利组比较差异无统计学意义(P>0.05),丹参多酚酸盐高剂量组大鼠上述蛋白表达的改善程度显著优于贝那普利组(P<0.01),丹参多酚酸盐对MN模型大鼠上述蛋白表达水平的改善作用具有明显的剂量依赖性(P<0.01)。结论:丹参多酚酸盐可能通过调控PERK/ATF4/CHOP信号通路,缓解肾组织内质网应激,抑制足细胞凋亡,进而保护肾脏及延缓疾病进展。 展开更多
关键词 膜性肾病 丹参多酚酸盐 内质网应激 PERK/atf4/CHOP信号通路 凋亡 盐酸贝那普利
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Step-over of strike-slip faults and overpressure fluid favor occurrence of foreshocks:Insights from the 1975 Haicheng fore-main-aftershock sequence,China 被引量:1
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作者 Xinglin Lei Zhiwei Wang +1 位作者 Shengli Ma Changrong He 《Earthquake Research Advances》 CSCD 2024年第1期36-46,共11页
This study analyzed and summarized in detail the spatial and temporal distributions of earthquakes,tidal responses,focal mechanisms,and stress field characteristics for the M 7.3 Haicheng earthquake sequence in Februa... This study analyzed and summarized in detail the spatial and temporal distributions of earthquakes,tidal responses,focal mechanisms,and stress field characteristics for the M 7.3 Haicheng earthquake sequence in February 1975.The foreshocks are related to the main fault and the conjugate faults surrounding the extension step-over in the middle.The initiation timing of the foreshock clusters and the original time of the mainshock were clearly modulated by the Earth's tidal force and coincided with the peak of dilational volumetric tidal strain.As a plausible and testable hypothesis,we proposed a fluid-driven foreshock model,by which all observed seismicity features can be more reasonably interpreted with respect to the results of existing models.Together with some other known examples,the widely existing step-over along strike-slip faults and associated conjugate faults,especially for extensional ones in the presence of deep fluids,favor the occurrence of short-term foreshocks.Although clustered seismicity with characteristics similar to those of the studied case is not a sufficient and necessary condition for large earthquakes to occur under similar tectonic conditions,it undoubtedly has a warning significance for the criticality of the main fault.Subsequent testing would require quantification of true/false positives/negatives. 展开更多
关键词 Haicheng earthquake Seismogenic fault ETAS FORESHOCK Deep fluid
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Reservoir quality evaluation of the Narimba Formation in Bass Basin,Australia:Implications from petrophysical analysis,sedimentological features,capillary pressure and wetting fluid saturation 被引量:1
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作者 Wafa Abdul Qader Al-Ojaili Mohamed Ragab Shalaby Wilfried Bauer 《Energy Geoscience》 EI 2024年第1期37-53,共17页
The evaluation of reservoir quality was accomplished on the Late Paleocene to Early Eocene Narimba Formation in Bass Basin,Australia.This study involved combination methods such as petrophysical analysis,petrography a... The evaluation of reservoir quality was accomplished on the Late Paleocene to Early Eocene Narimba Formation in Bass Basin,Australia.This study involved combination methods such as petrophysical analysis,petrography and sedimentological studies,reservoir quality and fluid flow units from derivative parameters,and capillary pressure and wetting fluid saturation relationship.Textural and diagenetic features are affecting the reservoir quality.Cementation,compaction,and presence of clay minerals such as kaolinite are found to reduce the quality while dissolution and secondary porosity are noticed to improve it.It is believed that the Narimba Formation is a potential reservoir with a wide range of porosity and permeability.Porosity ranges from 3.1%to 25.4%with a mean of 15.84%,while permeability ranges between 0.01 mD and 510 mD,with a mean of 31.05 mD.Based on the heterogenous lithology,the formation has been categorized into five groups based on permeability variations.Group I showed an excellent to good quality reservoir with coarse grains.The impacts of both textural and diagenetic features improve the reservoir and producing higher reservoir quality index(RQI)and flow zone indicators(FZI)as well as mostly mega pores.The non-wetting fluid migration has the higher possibility to flow in the formation while displacement pressure recorded as zero.Group II showed a fair quality reservoir with lower petrophysical properties in macro pores.The irreducible water saturation is increasing while the textural and digenetic properties are still enhancing the reservoir quality.Group III reflects lower quality reservoir with mostly macro pores and higher displacement pressure.It may indicate smaller grain size and increasing amount of cement and clay minerals.Group IV,and V are interpreted as a poor-quality reservoir that has lower RQI and FZI.The textural and digenetic features are negatively affecting the reservoir and are leading to smaller pore size and pore throat radii(r35)values to be within the range of macro,meso-,micro-,and nano pores.The capillary displacement pressure curves of the three groups show increases reaching the maximum value of 400 psia in group V.Agreement with the classification of permeability,r35 values,and pore type can be used in identifying the quality of reservoir. 展开更多
关键词 Narimba formation PETROPHYSICS Reservoir quality Capillary pressure Wetting fluid saturation
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Effect of bubble morphology and behavior on power consumption in non-Newtonian fluids’aeration process 被引量:1
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作者 Xiemin Liu Jing Wan +5 位作者 Jinnan Sun Lin Zhang Feng Zhang Zhibing Zhang Xinyao Li Zheng Zhou 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2024年第1期243-254,共12页
Due to a prolonged operation time and low mass transfer efficiency, the primary challenge in the aeration process of non-Newtonian fluids is the high energy consumption, which is closely related to the form and rate o... Due to a prolonged operation time and low mass transfer efficiency, the primary challenge in the aeration process of non-Newtonian fluids is the high energy consumption, which is closely related to the form and rate of impeller, ventilation, rheological properties and bubble morphology in the reactor. In this perspective, through optimal computational fluid dynamics models and experiments, the relationship between power consumption, volumetric mass transfer rate(kLa) and initial bubble size(d0) was constructed to establish an efficient operation mode for the aeration process of non-Newtonian fluids. It was found that reducing the d0could significantly increase the oxygen mass transfer rate, resulting in an obvious decrease in the ventilation volume and impeller speed. When d0was regulated within 2-5 mm,an optimal kLa could be achieved, and 21% of power consumption could be saved, compared to the case of bubbles with a diameter of 10 mm. 展开更多
关键词 Non-Newtonian fluids aeration process Power consumption Volumetric mass transfer rate Bubble size
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降脂通脉饮对高血脂症大鼠血脂和PERK/ATF4/CHOP信号通路的影响 被引量:1
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作者 郭锦荣 黄微 +3 位作者 吕丹丹 颜靖文 周芬敏 陆瑞峰 《现代中西医结合杂志》 CAS 2024年第4期465-468,479,共5页
目的观察降脂通脉饮对高血脂症大鼠血脂及蛋白激酶样内质网激酶(PERK)/转录激活因子4(ATF4)/C/EBP同源蛋白(CHOP)信号通路的影响,探讨降脂通脉饮调节血脂的作用机制。方法将30只SD大鼠随机分为正常组、模型组及降脂通脉饮低、中、高剂量... 目的观察降脂通脉饮对高血脂症大鼠血脂及蛋白激酶样内质网激酶(PERK)/转录激活因子4(ATF4)/C/EBP同源蛋白(CHOP)信号通路的影响,探讨降脂通脉饮调节血脂的作用机制。方法将30只SD大鼠随机分为正常组、模型组及降脂通脉饮低、中、高剂量组,每组6只。正常组大鼠给予普通饲料喂养,同时灌胃生理盐水;其余组大鼠给予高脂饲料喂养,其中模型组同时灌胃生理盐水,降脂通脉饮低、中、高剂量组同时灌胃1.56 mg/(kg·d)、3.125 mg/(kg·d)、6.25 mg/(kg·d)的降脂通脉饮。连续干预8周后,生化法检测血清胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)水平,RT-PCR和Western blot法检测颈动脉组织中PERK、ATF4、CHOPmRNA及蛋白表达情况。结果与正常组比较,模型组大鼠血清TC、TG、LDL-C水平和PERK、ATF4、CHOP mRNA及蛋白相对表达量均明显升高(P均<0.05);与模型组比较,降脂通脉饮中、高剂量组大鼠血清TC、TG、LDL-C水平和PERK、ATF4、CHOP mRNA及蛋白相对表达量均明显降低(P均<0.05)。结论降脂通脉饮可能通过调节内质网应激PERK/ATF4/CHOP信号通路而发挥降脂作用。 展开更多
关键词 高血脂症 降脂通脉饮 PERK/atf4/CHOP信号通路
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Endoscopic intramural cystogastrostomy for treatment of peripancreatic fluid collection: A viewpoint from a surgeon 被引量:1
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作者 Chen-Guo Ker 《World Journal of Gastroenterology》 SCIE CAS 2024年第6期610-613,共4页
Percutaneous or endoscopic drainage is the initial choice for the treatment of peripancreatic fluid collection in symptomatic patients.Endoscopic transgastric fenestration(ETGF)was first reported for the management of... Percutaneous or endoscopic drainage is the initial choice for the treatment of peripancreatic fluid collection in symptomatic patients.Endoscopic transgastric fenestration(ETGF)was first reported for the management of pancreatic pseu-docysts of 20 patients in 2008.From a surgeon’s viewpoint,ETGF is a similar procedure to cystogastrostomy in that they both produce a wide outlet orifice for the drainage of fluid and necrotic debris.ETGF can be performed at least 4 wk after the initial onset of acute pancreatitis and it has a high priority over the surgical approach.However,the surgical approach usually has a better success rate because surgical cystogastrostomy has a wider outlet(>6 cm vs 2 cm)than ETGF.However,percutaneous or endoscopic drainage,ETGF,and surgical approach offer various treatment options for peripancreatic fluid collection patients based on their conditions. 展开更多
关键词 Pancreatitis Pancreatic pseudocyst Endoscopic cystogastrostomy Surgical cystogastrostomy Peripancreatic fluid collection Fenestration for pancreatic cyst
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An inverse analysis of fluid flow through granular media using differentiable lattice Boltzmann method 被引量:1
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作者 Qiuyu Wang Krishna Kumar 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第6期2077-2090,共14页
This study presents a method for the inverse analysis of fluid flow problems.The focus is put on accurately determining boundary conditions and characterizing the physical properties of granular media,such as permeabi... This study presents a method for the inverse analysis of fluid flow problems.The focus is put on accurately determining boundary conditions and characterizing the physical properties of granular media,such as permeability,and fluid components,like viscosity.The primary aim is to deduce either constant pressure head or pressure profiles,given the known velocity field at a steady-state flow through a conduit containing obstacles,including walls,spheres,and grains.The lattice Boltzmann method(LBM)combined with automatic differentiation(AD)(AD-LBM)is employed,with the help of the GPU-capable Taichi programming language.A lightweight tape is used to generate gradients for the entire LBM simulation,enabling end-to-end backpropagation.Our AD-LBM approach accurately estimates the boundary conditions for complex flow paths in porous media,leading to observed steady-state velocity fields and deriving macro-scale permeability and fluid viscosity.The method demonstrates significant advantages in terms of prediction accuracy and computational efficiency,making it a powerful tool for solving inverse fluid flow problems in various applications. 展开更多
关键词 Inverse problem fluid flow Granular media Automatic differentiation(AD) Lattice Boltzmann method(LBM)
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Application and prospect of the fluid cooling system of solar arrays for probing the Sun 被引量:1
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作者 Kangli Bao Xiaofei Zhu +5 位作者 Jianchao Feng Liu Liu Xiaofeng Zhang Zhiming Cai Jun Lin Yonghe Zhang 《Astronomical Techniques and Instruments》 CSCD 2024年第1期62-70,共9页
The Solar Close Observations and Proximity Experiments(SCOPE)mission,which has been proposed by the Yunnan Observatories,Chinese Academy of Sciences,aiming to operate at a distance of 5 to 10 solar radii from the Sun,... The Solar Close Observations and Proximity Experiments(SCOPE)mission,which has been proposed by the Yunnan Observatories,Chinese Academy of Sciences,aiming to operate at a distance of 5 to 10 solar radii from the Sun,plans to complete the in situ detection of the solar eruption process and observation of the magnetic field structure response.The solar flux received by the satellite ranges from 10^(3) to 10^(6) Wm^(-2),which poses challenges for thermal management of the solar arrays.In this work,the solar array cooling system of the Parker Solar Probe is discussed,the developments of the fluid loop technique are reviewed,and a research plan for a next-generation solar array cooling system is proposed.This paper provides a valuable reference for novel thermal control systems in spacecraft for solar observation. 展开更多
关键词 In situ detection of solar eruption Solar array cooling system Pumped fluid loop High heat flux dissipation
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The Conversion of Non-Dispersed Polymers into Low-Potassium Anti-Collapse Drilling Fluids
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作者 Hao Hu Jian Guan +2 位作者 Shanfa Tang Jialuo Rong Yuanpeng Cheng 《Fluid Dynamics & Materials Processing》 EI 2024年第2期325-335,共11页
Different drillingfluid systems are designed according to mineral composition,lithology and wellbore stability of different strata.In the present study,the conversion of a non-dispersed polymer drillingfluid into a low ... Different drillingfluid systems are designed according to mineral composition,lithology and wellbore stability of different strata.In the present study,the conversion of a non-dispersed polymer drillingfluid into a low potas-sium anti-collapsing drillingfluid is investigated.Since the two drillingfluids belong to completely different types,the key to this conversion is represented by new inhibitors,dispersants and water-loss agents by which a non-dispersed drillingfluid can be turned into a dispersed drillingfluid while ensuring wellbore stability and reason-able rheology(carrying sand—inhibiting cuttings dispersion).In particular,the(QYZ-1)inhibitors and(FSJSS-2)dispersants are used.The former can inhibit the hydration expansion capacity of clay,reduce the dynamic shear force and weaken the viscosity;the latter can improve the sealing effect and reduce thefiltrate loss.The results have shown that after adding a reasonable proportion of these substances(QYZ-1:FSJSS-2)to the non-dispersed polymer drillingfluid,while the apparent viscosity,plastic viscosity,structural viscosity andfluidity index under-went almost negligible changes,the dynamic plastic ratio increased,and thefiltration loss decreased significantly,thereby indicating good compatibility.According to the tests(conducted in the Leijia area),the density was 1.293 g/cm3,and after standing for 24 h,the SF(static settlement factor)was 0.51.Moreover,thefiltration loss was reduced to 4.0 mL,the rolling recovery rate reached 96.92%,with excellent plugging and anti-collapse performances. 展开更多
关键词 Non-dispersed polymer drilling fluid low potassium anti-collapsing drilling fluid drilling fluid conversion drilling fluid reuse filter vector
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Imaging the Architecture of Mineral Systems and the Pathways of Ore-forming Fluids across Mongolia with Magnetotellurics
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作者 Matthew J.COMEAU Rafael RIGAUD +4 位作者 Erdenechimeg BATMAGNAI Shoovdor TSERENDUG Sodnomsambuu DEMBEREL Michael BECKEN Alexey KUVSHINOV 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2024年第S01期11-13,共3页
In the framework of a mineral system approach,a combination of components is required to develop a mineral system.This includes the whole-lithosphere architecture,which controls the transport of ore-forming fluids,and... In the framework of a mineral system approach,a combination of components is required to develop a mineral system.This includes the whole-lithosphere architecture,which controls the transport of ore-forming fluids,and favorable tectonic and geodynamic processes,occurring at various spatial and temporal scales,that influence the genesis and evolution of ore-forming fluids(Huston et al.,2016;Groves et al.,2018;Davies et al.,2020).Knowledge of the deep structural framework can advance the understanding of the development of a mineral system and the emplacement of mineral deposits.Deep geophysical exploration carried out with this aim is increasingly important for targeting new ore deposits in unexplored and underexplored regions(Dentith et al.,2018;Dentith,2019). 展开更多
关键词 MAGNETOTELLURICS electrical resistivity mineral exploration mineral emplacement ORE fluids fluid transport
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基于PERK/eIF2α/ATF4/CHOP通路探讨健骨颗粒对UMR-106细胞内质网应激凋亡的影响 被引量:1
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作者 陈赛楠 周芬 +1 位作者 黄云梅 林燕萍 《康复学报》 CSCD 2024年第1期34-43,共10页
目的探讨健骨颗粒含药血清对UMR-106成骨样细胞内质网应激ERS凋亡的影响和作用机制。方法选用UMR-106成骨样细胞,采用0、0.01、0.02、0.03、0.04、0.05μmol/L不同浓度的GSK2606414(PERK抑制剂)对细胞进行干预,采用CCK8法筛选GSK260641... 目的探讨健骨颗粒含药血清对UMR-106成骨样细胞内质网应激ERS凋亡的影响和作用机制。方法选用UMR-106成骨样细胞,采用0、0.01、0.02、0.03、0.04、0.05μmol/L不同浓度的GSK2606414(PERK抑制剂)对细胞进行干预,采用CCK8法筛选GSK2606414最佳干预浓度。将生长状态较好的UMR-106细胞随机分为阴性对照组(NC组)、模型组(H_(2)O_(2)组)、健骨颗粒组(H_(2)O_(2)+JG组)和阳性对照组(H_(2)O_(2)+GSK2606414组)4组。NC组和H_(2)O_(2)组采用10%生理盐水血清干预12 h,H_(2)O_(2)+JG组采用10%健骨颗粒含药血清干预12 h,H_(2)O_(2)+GSK2606414组采用0.03μmol/L的GSK2606414和10%生理盐水血清干预12 h,除NC组外,其余各组在不更换新培养基的情况下,再分别加入10μmol/L H_(2)O_(2)干预12 h。采用激光共聚焦显微镜观察细胞内NC组和H_(2)O_(2)组GRP78和Caspase-12荧光表达情况,DCFH-DA检测活性氧(ROS)含量,激光共聚焦显微镜观察细胞内钙离子实时动态变化判断ROS/ERS模型是否成立。再采用An⁃nexin V-FITC/PI检测4组细胞晚期凋亡率,qPCR和Western blot检测4组ERS相关标志指标GRP78、PERK、eIf2α、ATF4和CHOP mRNA转录水平和蛋白相对表达量。结果与0μmol/L组比较,0.03μmol/L的GSK2606414是干预UMR-106细胞12 h后对细胞活力没有影响的最大浓度,故使用该浓度作为后续H_(2)O_(2)+GSK2606414组的实验干预条件。与NC组相比,H_(2)O_(2)组ROS含量显著增高(P<0.01),GRP78和Caspase-12的蛋白荧光表达量明显增加,细胞内钙离子流动速度加快并持续增高,表明H_(2)O_(2)诱导UMR-106细胞ROS/ERS模型的成功建立。与NC组相比,H_(2)O_(2)组凋亡率显著增高(P<0.05),GRP78、PERK、eIf2α、ATF4、CHOP mRNA转录水平和蛋白相对表达量均显著增高(P<0.01)。与H_(2)O_(2)组相比,H_(2)O_(2)+JG组和H_(2)O_(2)+GSK2606414组ROS含量显著降低(P<0.01),凋亡率显著降低(P<0.05),GRP78、PERK、eIf2α、ATF4、CHOP mRNA转录水平(P<0.05)和蛋白相对表达量(P<0.01)均显著降低。结论健骨颗粒可通过PERK/eIF2α/ATF4/CHOP信号通路缓解内质网过度应激,降低成骨细胞凋亡率,发挥防治绝经后骨质疏松症的作用。 展开更多
关键词 绝经后骨质疏松症 内质网应激 健骨颗粒 PERK/eIF2α/atf4/CHOP信号通路 成骨细胞凋亡
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