Contaminated surfaces play a significant role in the transmission of respiratory infectious diseases.To address this issue,we pre-sented a novel quantitative detection method for droplets on physical surfaces,based on...Contaminated surfaces play a significant role in the transmission of respiratory infectious diseases.To address this issue,we pre-sented a novel quantitative detection method for droplets on physical surfaces,based on the laser-induced fluorescence technique.The proposed detection method was demonstrated in a realistic high-speed train compartment scenario by simulating the process of droplet release during passengers’breathing and coughing.The experimental results showed that this method could offer high precision(10-1 mg/m^(2))for detecting minute substance concentrations,and its ease of operation makes it suitable for complex en-gineering environments.The results also revealed that under the combined effects of the indoor airflow and breathing airflow,the range of droplets released by breathing activity exceeded two rows in front of and behind the release position.Simultaneously,we observed that a large number of droplets settled on the seat surfaces on both sides of the same row as the releaser,with over 36%of these droplets concentrated on the backrest area of the seats.As the respiratory jet velocity increased,the location with the most sed-iment droplets(accounting for 8%of the total sedimentation)occurred on the seat directly in front of the releaser,and approximately 48% of the droplets were found on the back of this seat.Our proposed method overcomes the shortcomings of existing experimental methods in quantitatively capturing the motion characteristics of droplets in complex flow fields.展开更多
Aims:Triple-negative breast cancer patients are commonly treated with combination chemotherapy.Nonetheless,outcomes remain substandard with relapses being of a frequent occurrence.Among the several mechanisms that res...Aims:Triple-negative breast cancer patients are commonly treated with combination chemotherapy.Nonetheless,outcomes remain substandard with relapses being of a frequent occurrence.Among the several mechanisms that result in treatment failure,multidrug resistance,which is mediated by ATP-binding cassette proteins,is the most common.Regardless of the substantial studies conducted on the heterogeneity of cancer types,only a few assays can distinguish the variability in multidrug resistance activity between individual cells.We aim to develop a single-cell assay to study this.Methods:This experiment utilized a microfluidic chip to measure the drug accumulation in single breast cancer cells in order to understand the inhibition of drug efflux properties.Results:Selection of single cells,loading of drugs,and fluorescence measurement for intracellular drug accumulation were all conducted on a microfluidic chip.As a result,measurements of the accumulation of chemotherapeutic drugs(e.g.,daunorubicin and paclitaxel)in single cells in the presence and absence of cyclosporine A were conducted.Parameters such as initial drug accumulation,signal saturation time,and fold-increase of drug with and without the presence cyclosporine A were also tested.Conclusion:The results display that drug accumulation in a single-cell greatly enhanced over its same-cell control because of inhibition by cyclosporine A.Furthermore,this experiment may provide a platform for future liquid biopsy studies to characterize the multidrug resistance activity at a single-cell level.展开更多
Staphylococcus aureus(S.aureus)has been identified as one of the major foodborne pathogenic bacteria.The development of rapid detection methods for S.aureus is needed for assuring food safety.In this study,quantum dot...Staphylococcus aureus(S.aureus)has been identified as one of the major foodborne pathogenic bacteria.The development of rapid detection methods for S.aureus is needed for assuring food safety.In this study,quantum dots were used as fluorescent labels in an immunoassay for quantitative detection of S.aureus.Firstly,biotin-labeled anti-S.aureus antibody was conjugated with streptavidin-coated magnetic nanobeads(180 nm diameter)and used to separate S.aureus cells.Then streptavidin coated quantum dots(QDs)were conjugated with biotin-labeled anti-S.aureus antibody and used as the fluorescence labels to mix with the separated S.aureus.Finally the fluorescence intensity of the bead-cell-QD complexes was measured at a wavelength of 620 nm.A linear relationship between S.aureus cell number(X)and fluorescence intensity(Y)was found for cell numbers ranging from 10^(3) to 10^(6) CFU(Colony Forming Unit)/mL,and the detection limit was 10^(3) CFU/mL.The regression model can be expressed as Y=7.68X+35.06 with R^(2)=0.94.The detection of S.aureus in food sample was explored initially.The fluorescence intensity of food sample was close to the background,so it was not satisfied.Further study will focus on the application of the method for detection of S.aureus in food sample.展开更多
基金supported by the National Natural Science Foun-dation of China(Grant No.52072413)the graduate school of Central South University(Grant No.1053320213788).
文摘Contaminated surfaces play a significant role in the transmission of respiratory infectious diseases.To address this issue,we pre-sented a novel quantitative detection method for droplets on physical surfaces,based on the laser-induced fluorescence technique.The proposed detection method was demonstrated in a realistic high-speed train compartment scenario by simulating the process of droplet release during passengers’breathing and coughing.The experimental results showed that this method could offer high precision(10-1 mg/m^(2))for detecting minute substance concentrations,and its ease of operation makes it suitable for complex en-gineering environments.The results also revealed that under the combined effects of the indoor airflow and breathing airflow,the range of droplets released by breathing activity exceeded two rows in front of and behind the release position.Simultaneously,we observed that a large number of droplets settled on the seat surfaces on both sides of the same row as the releaser,with over 36%of these droplets concentrated on the backrest area of the seats.As the respiratory jet velocity increased,the location with the most sed-iment droplets(accounting for 8%of the total sedimentation)occurred on the seat directly in front of the releaser,and approximately 48% of the droplets were found on the back of this seat.Our proposed method overcomes the shortcomings of existing experimental methods in quantitatively capturing the motion characteristics of droplets in complex flow fields.
文摘Aims:Triple-negative breast cancer patients are commonly treated with combination chemotherapy.Nonetheless,outcomes remain substandard with relapses being of a frequent occurrence.Among the several mechanisms that result in treatment failure,multidrug resistance,which is mediated by ATP-binding cassette proteins,is the most common.Regardless of the substantial studies conducted on the heterogeneity of cancer types,only a few assays can distinguish the variability in multidrug resistance activity between individual cells.We aim to develop a single-cell assay to study this.Methods:This experiment utilized a microfluidic chip to measure the drug accumulation in single breast cancer cells in order to understand the inhibition of drug efflux properties.Results:Selection of single cells,loading of drugs,and fluorescence measurement for intracellular drug accumulation were all conducted on a microfluidic chip.As a result,measurements of the accumulation of chemotherapeutic drugs(e.g.,daunorubicin and paclitaxel)in single cells in the presence and absence of cyclosporine A were conducted.Parameters such as initial drug accumulation,signal saturation time,and fold-increase of drug with and without the presence cyclosporine A were also tested.Conclusion:The results display that drug accumulation in a single-cell greatly enhanced over its same-cell control because of inhibition by cyclosporine A.Furthermore,this experiment may provide a platform for future liquid biopsy studies to characterize the multidrug resistance activity at a single-cell level.
基金This research was financially supported by the Fundamental Research Funds for the Central Universities of China(No.QN2011144)the Yangling Modern Agriculture International Institute(No.A213021005).
文摘Staphylococcus aureus(S.aureus)has been identified as one of the major foodborne pathogenic bacteria.The development of rapid detection methods for S.aureus is needed for assuring food safety.In this study,quantum dots were used as fluorescent labels in an immunoassay for quantitative detection of S.aureus.Firstly,biotin-labeled anti-S.aureus antibody was conjugated with streptavidin-coated magnetic nanobeads(180 nm diameter)and used to separate S.aureus cells.Then streptavidin coated quantum dots(QDs)were conjugated with biotin-labeled anti-S.aureus antibody and used as the fluorescence labels to mix with the separated S.aureus.Finally the fluorescence intensity of the bead-cell-QD complexes was measured at a wavelength of 620 nm.A linear relationship between S.aureus cell number(X)and fluorescence intensity(Y)was found for cell numbers ranging from 10^(3) to 10^(6) CFU(Colony Forming Unit)/mL,and the detection limit was 10^(3) CFU/mL.The regression model can be expressed as Y=7.68X+35.06 with R^(2)=0.94.The detection of S.aureus in food sample was explored initially.The fluorescence intensity of food sample was close to the background,so it was not satisfied.Further study will focus on the application of the method for detection of S.aureus in food sample.
