To enhance gas drainage in the soft and hard interbedded(SHI)coal seam,it’s necessary to unload the insitu stress and improve its gas migration performance.In this research,a directional hydraulic flushing(DHF)techno...To enhance gas drainage in the soft and hard interbedded(SHI)coal seam,it’s necessary to unload the insitu stress and improve its gas migration performance.In this research,a directional hydraulic flushing(DHF)technology was carried out.The stress-unloading and gas migration improvement mechanism was analyzed through numerical simulation,and systematic engineering tests were conducted to verify the gas drainage effect.The results show that the improvement of gas migration performance in the SHI coal seam is caused by a combined effect of seepage-improving and diffusion-improving.After DHF,stress-unloading and plastic failure could be achieved both in the soft coal(SC)sublayer and in the hard coal(HC)sublayer.However,the gas diffusion capacity improves significantly in the SC sublayer,while the gas seepage capacity improves notably in the HC sublayer.Meanwhile,the stress-unloading and gas migration improvement effect improves with the flushing radius and the thickness of the SC sublayer.Besides,after adopting the DHF technology,the gas drainage effect improved markedly.The borehole number dropped by 49%,the gas drainage ratio increased from 26.0%to 48.2%,and the average coal roadway excavation speed increased from 2.4 to 5.6 m/d.展开更多
The actuator is a key component of the creaming tool in drilling applications.Its jet performances determine the effective reaming efficiency.In this work,a new selective reaming tool is proposed and the RNG k-εturbu...The actuator is a key component of the creaming tool in drilling applications.Its jet performances determine the effective reaming efficiency.In this work,a new selective reaming tool is proposed and the RNG k-εturbulence model is used to calculate its internal and externalflowfields.In particular,special attention is paid to the design of theflushing nozzle.The results show that the jet originating from theflushing nozzle has a significant influence on rock cutting and blade cooling effects.In turn,the jet performances depend on geometric structure of the creaming actuator.In this framework,a conical-cylindrical nozzle with a diameter of 7 mm is initially considered as a basis to implement a strategy to optimize the structural parameters of the reaming actuator,and improve the related side tracking reconstruction technology.展开更多
BACKGROUND Vancomycin flushing syndrome(VFS),also known as red man syndrome,is an allergic reaction to vancomycin.It typically presents as a rash on the face,neck,and upper torso after intravenous administration of va...BACKGROUND Vancomycin flushing syndrome(VFS),also known as red man syndrome,is an allergic reaction to vancomycin.It typically presents as a rash on the face,neck,and upper torso after intravenous administration of vancomycin.VFS is blamed on rapid intravenous infusion of vancomycin during management and rarely happens after local use.A review of the literature showed that in the last 23 years,4 such cases have been reported.Here,we add another case of VFS developed after slow local absorption of vancomycin in cement beads.CASE SUMMARY A 44-year-old male with a known case of hypertension,no history of allergies to medications,and a history of chronic osteomyelitis of the right tibia with discharging sinus over the anterolateral aspect of the leg.The pus culture grew Staphylococcus aureus,which was sensitive to clindamycin and vancomycin.The patient underwent irrigation and debridement with the placement of vancomycin cement beads made from 4 g of vancomycin powder and 40 g of polymethyl methacrylate.Three hours postoperatively,the patient developed a pruritic,erythematous,macular rash predominantly on his face,neck,chest,and lower extremities and to a lesser extent his upper extremities.A diagnosis of VFS was made and was successfully treated with cetirizine(10 mg,oral)and methylprednisolone sodium succinate(125 mg,intravenous).The patient continued to have itching with a facial rash for 12 h with gradual improvement.A decision was made to not remove the beads as the patient continued to improve.Gradually,the rash disappeared after 96 h with no further sequela.CONCLUSION VFS can occur not only after rapid intravenous injection of vancomycin but also with local release,as in our case.As orthopaedic surgeons routinely use vancomycin with polymethyl methacrylate in chronic osteomyelitis and revision arthroplasty,they should be aware of such a complication occurring.展开更多
Field experiments were carried out at the National Research Center farm, Nubaria area, Behura Governorate, Egypt, to study the effect of nitrogen source, flushing schedule and irrigation method on emitter clogging. Pe...Field experiments were carried out at the National Research Center farm, Nubaria area, Behura Governorate, Egypt, to study the effect of nitrogen source, flushing schedule and irrigation method on emitter clogging. Peanut Giza 5 variety (Arachishy pogaea L.) was planted in sandy soil during two successive growing seasons (2010-2011) in the 1st week of May and harvested after 130 days. Treatments used are: 1) two irrigation methods: surface drip irrigation and sub-surface drip irrigation (SDI;SSDI), 2) nitrogen source (NS):NH4NO3, (NH4)2SO4 and Ca(NO3)2 (NS1, NS2 and NS3) and 3) flushing number (FL) 0, 1 and 4 (FL1, FL2;FL3). The experiments design was split-split plot and three replicates were used. Data obtained were subjected to statistical analysis. The main effects of treatments used on clogging per cent could be written the following ascending orders: SDI 3 2 1, NS1 2 3. Concerning the 1st interaction the following ascending orders denote their effects on clogging percent: SDI × FL3 2 1, SDI × NS1 2 3, SSDI × FL3 2 1, SSDI × NS1 2 3, FL1 × NS1 1 × NS2 1 × NS3, FL2 × NS1 2 × NS2 2 × NS3 and FL3 × NS1 3 × NS2 3 × NS3. The differences between any two treatments and/or any two interactions in clogging percent were significant at the 5% level. The effect of the 2nd interaction on clogging percent was significant at the 5% level. The maximum value of clogging (20.18%) and the lowest one (3.9%) were archived in the interactions: SSDI × FL1 × NS3 and SDI × FL3 × NS1, respectively.展开更多
The activity of matrix metalloproteinases (MMPs) in the uterine flushing and endometrial tissue of normal adult women wearing FCu IUD (fixed Cu IUD) or FICu IUD (indomethacin releasing FCu IUD) was observed by using z...The activity of matrix metalloproteinases (MMPs) in the uterine flushing and endometrial tissue of normal adult women wearing FCu IUD (fixed Cu IUD) or FICu IUD (indomethacin releasing FCu IUD) was observed by using zymography on SDS PAGE containing gelatin. The results showed that the activity and kinds of MMPs in FCu IUD group were increased significantly as compared with themselves before being inserted FCu IUD. However, compared with the FCu IUD group, the activity of some kinds of MMPs in the FICu IUD group was decreased significantly. These data suggest that IUD can enhance the activity of MMPs in human endometrium, intermediated by prostaglandins, and MMPs may have relation to IUD induced menorrhagia and indomethacin reduces IUD induced menorrhagia by partly inhibiting MMPs synthesis.展开更多
This paper analyzes the situation at the Iffezheim barrage along the fiver Rhine.Since the start of operation in 1977 several million cubic meters of very fine sediment have been deposited in the reservoir.Be- cause o...This paper analyzes the situation at the Iffezheim barrage along the fiver Rhine.Since the start of operation in 1977 several million cubic meters of very fine sediment have been deposited in the reservoir.Be- cause of stability problems and reduced safety in the event of flooding,an extraction of the material is neces- sary.Chemical assays showed a high concentration of HCB.Therefore flushing without any additional measures is not appropriate.In order to leam more about the morphological and chemical in...展开更多
Objective: To discuss the effect of vacuum sealing drainage combined with intermittent insulin flushing on the expression of inflammatory factors and apoptosis factors in diabetic lower limb ulcer wound. Methods: A to...Objective: To discuss the effect of vacuum sealing drainage combined with intermittent insulin flushing on the expression of inflammatory factors and apoptosis factors in diabetic lower limb ulcer wound. Methods: A total of 48 patients with diabetic lower limb ulcer who were treated in the Endocrine Department of the hospital between January 2016 and January 2017 were divided into the control group and research group by random number table, 24 cases in each group. Control group received vacuum sealing drainage, and research group received vacuum sealing drainage combined with intermittent insulin flushing. The differences in the expression of inflammatory factors and apoptosis genes in the wound exudate were compared between the two groups before and after treatment. Results: Before treatment, the differences in the inflammatory factor contents and apoptsis gene expression in wound exudate were not statistically significant between the two groups of patients. After treatment, inflammatory factors IL-6, IL-1β, TNF-α, CRP, VCAM-1 and FGF2 contents in wound exudate of research group were lower than those of control group;apoptosis gene Bcl-2 mRNA expression in wound exudate of research group was higher than that of control group while Bax, p53 and Fas mRNA expression were lower than those of control group. Conclusions: Vacuum sealing drainage combined with intermittent insulin flushing can effectively reduce the local inflammatory response and promote the granulation cell proliferation in wound of patients with diabetic lower limb ulcer.展开更多
In-site soil flushing and aeration are the typical synergetic remediation technology for contaminated sites.The surfactant present in flushing solutions is bound to affect the aeration efficiency.The purpose of this s...In-site soil flushing and aeration are the typical synergetic remediation technology for contaminated sites.The surfactant present in flushing solutions is bound to affect the aeration efficiency.The purpose of this study is to evaluate the effect of surfactant frequently used in soil flushing on the oxygen mass transfer in micro-nano-bubble(MNB)aeration system.Firstly,bio-surfactants and chemical surfactants were used to investigate their effects on Sauter mean diameter of bubble(dBS),gas holdup(ε),volumetric mass-transfer coefficient(kLa)and liquid-side mass-transfer coefficient(kL)in the MNB aeration system.Then,based upon the experimental results,the Sardeing's and Fr€ossling's models were modified to describe the effect of surfactant on kL in the MNB aeration.The results showed that,for the twenty aqueous surfactant solutions,with the increase in surfactant concentration,the value of dBS,kLa and kL decreased,while the value ofεand gas-liquid interfacial area(a)increased.These phenomena were mainly attributed to the synergistic effects of immobile bubble surface and the suppression of coalescence in the surfactant solutions.In addition,with the presence of electric charge,MNBs in anionic surfactant solutions were smaller and higher in number than in non-ionic surfactant solutions.Furthermore,the accumulation of surfactant on the gaseliquid interface was more conspicuous for small MNB,so the reduction of kL in anionic surfactant solutions was larger than that in non-ionic surfactant solutions.Besides,the modified Fr€ossling's model predicted the effect of surfactant on kL in MNB aeration system with reasonable accuracy.展开更多
Capella油田是哥伦比亚南部重要的稠油油田,目前处于开发生产阶段,为了油田的稳产增产,需频繁对油井进行干预。Flush by Unit具有操作简单、移动高效、作业时间缩短、安全事故少等优点,作为试点引入了Flush by Unit为该油田的浅井提供...Capella油田是哥伦比亚南部重要的稠油油田,目前处于开发生产阶段,为了油田的稳产增产,需频繁对油井进行干预。Flush by Unit具有操作简单、移动高效、作业时间缩短、安全事故少等优点,作为试点引入了Flush by Unit为该油田的浅井提供修井服务,以降低桶油成本。本文介绍了Flush by Unit的结构组成、使用范围、现场使用效果,与传统修井机相比,可以大幅降低作业时间和成本,为后续规模使用Flush by Unit进行常规修井提供了依据,也为油田的增产稳产带来了机遇。展开更多
AIM: To characterize the influence of location, species and treatment upon RNA degradation in tissue samples from the gastrointestinal tract. METHODS: The intestinal samples were stored in different medium for differe...AIM: To characterize the influence of location, species and treatment upon RNA degradation in tissue samples from the gastrointestinal tract. METHODS: The intestinal samples were stored in different medium for different times under varyingconditions: different species(human and rat), varying temperature(storage on crushed ice or room temperature), time point of dissection of the submucous-mucous layer from the smooth muscle(before or after storage), different rinsing methods(rinsing with Medium, PBS, RNALater or without rinsing at all) and different regions of the gut(proximal and distal small intestine, caecum, colon and rectum). The total RNA from different parts of the gut(rat: proximal and distal small intestine, caecum, colon and rectum, human: colon and rectum) and individual gut layers(muscle and submucosal/mucosal) was extracted. The quality of the RNA was assessed by micro capillary electrophoresis. The RNA quality was expressed by the RNA integrity number which is calculated from the relative height and area of the 18 S and 28 S RNA peaks. From rat distal small intestine q PCR was performed for neuronal and glial markers.RESULTS: RNA obtained from smooth muscle tissue is much longer stable than those from submucosal/mucosal tissue. At RT muscle RNA degrades after one day, on ice it is stable at least three days. Cleaning and separation of gut layers before storage and use of RNALater, maintains the stability of muscle RNA at RT for much longer periods. Different parts of the gut show varying degradation periods. RNA obtained from the submucosal/mucosal layer always showed a much worse amplification rate than RNA from muscle tissue. In general RNA harvested from rat tissue, either smooth muscle layer or submucosal/mucosal layer is much longer stable than RNA from human gut tissue, and RNA obtained from smooth muscle tissue shows an increased stability compared to RNA from submucosal/mucosal tissue. At RT muscle RNA degrades after one day, while the stability on ice lasts at least three days. Cleaning and separation of gut layers before storage and use of RNALater, maintains the stability of muscle RNA at RT for much longer periods. Different parts of the gut show varying degradation periods. The RNA from muscle and submucosal/mucosal tissue of the proximal small intestine degrades much faster than the RNA of distal small intestine, caecum or colonwith rectum. RNA obtained from the submucosal/mucosal layer always showed a much more reduced amplification rate than RNA from muscle tissue [β-Tubulin Ⅲ for muscle quantification cycle(Cp): 22.07 ± 0.25, for β-Tubulin Ⅲ submucosal/mucosal Cp: 27.42 ± 0.19].CONCLUSION: Degradation of intestinal m RNA depends on preparation and storage conditions of the tissue. Cooling, rinsing and separating of intestinal tissue reduce the degradation of m RNA.展开更多
Electrical discharge milling(ED-milling) can be a good choice for titanium alloys machining and it was proven that its machining efficiency can be improved to compete with mechanical cutting. In order to improve energ...Electrical discharge milling(ED-milling) can be a good choice for titanium alloys machining and it was proven that its machining efficiency can be improved to compete with mechanical cutting. In order to improve energy utilization efficiency of ED-milling process, unstable arc discharge and stable arc discharge combined with normal discharge were implemented for material removal by adjusting servo control strategy. The influence of electrode rotating speed and dielectric flushing pressure on machining performance was investigated by experiments. It was found that the rotating of electrode could move the position of discharge plasma channel, and high pressure flushing could wash melted debris out the discharge gap effectively. Both electrode rotating motion and high pressure flushing are contributed to the improvement of machining efficiency.展开更多
Background: Nutrition is one of the most important factors that affect animal performance, and it therefore also impacts on financial results in beef systems. In this way, finding the best strategy for feeding supplem...Background: Nutrition is one of the most important factors that affect animal performance, and it therefore also impacts on financial results in beef systems. In this way, finding the best strategy for feeding supplements is of paramount importance. Aiming to evaluate the effect of supplement feeding strategies for beef cows in the last third of gestation, two experiments were conducted. In Experiment 1, 35 pregnant Nellore cows were assigned to a completely randomized design with four treatments: control, which received no supplement; supplementation for the last 30 d of gestation(30-d; 3.0 kg/d); supplementation for the last 60 d of gestation(60-d; 1.5 kg/d); or supplementation for the last 90 d of gestation(90-d; 1.0 kg/d). All supplemented treatments received the same total amount of supplement throughout the experiment: 90 kg(20% of crude protein). A second experiment(Experiment 2) was delineated to evaluate the effects of the amounts offered in Experiment 1 on intake and metabolism. Four multiparous pregnant Nellore cows were assigned to a 4 × 4 Latin square design, with periods of15 d each.Results: There was a linear effect of the number of days of supplementation on calving body weight(BW; P < 0.05)and a quadratic effect on BW change from parturition to d 31 post-calving(P < 0.05), with cows on the 60-d strategy losing less BW post-calving. No difference was found in offspring birth BW(P > 0.10). A significant linear effect on interval from parturition to conception(P < 0.05) was observed, with the highest calving to conception interval being observed in the 90-d strategy. The level of supplementation did not affect forage intake or neutral detergent fiber digestibility(P > 0.10). Nitrogen excreted through urine tended to increase linearly with the level of supplementation(P < 0.10).Conclusion: Providing 1.5 kg of supplement during the last 60 d of gestation improves cow performance after calving, reducing the magnitude of BW lost, and reduces the number of days from calving to re-conception in the following breeding season compared to the usually recommended period of supplementation of 90 d pre-partum.展开更多
Conductive or send-conductive powder added into dielectric fluid can obviously lower the roughness of workpiece. With a mathematical ed developed to show the movement of dielectric fluid and powder particles in the ga...Conductive or send-conductive powder added into dielectric fluid can obviously lower the roughness of workpiece. With a mathematical ed developed to show the movement of dielectric fluid and powder particles in the gap flow field and a numerical algorithm proposed for the model, the distribution of powder particle conentration in the gap flow field is simulated with different jets for EDM.展开更多
High concentration and large flow flux of gas drainage from underground coal seams is the precondition of reducing emission and large-scale use of gas.However,the layered occurrence of coal seams with tectonically def...High concentration and large flow flux of gas drainage from underground coal seams is the precondition of reducing emission and large-scale use of gas.However,the layered occurrence of coal seams with tectonically deformed sub-layers and intact sub-layers makes it difficult to effectively drain gas through commonly designed boreholes.In this study,the gas drainage performance in coal seams with different combinations of tectonically deformed sub-layers and intact sub-layers was numerically analyzed.The analysis results show that the gas drainage curve changes from a single-stage line to a dual-stage curve as the permeability ratios of Zone II(kII)and Zone I(kI)increase,raising the difficulty in gas drainage.Furthermore,a dual-system pressure decay model based on the first-order kinetic model was developed to describe the dual-stage characteristics of pressure decay curves with different permeability ratios.In the end,the simulation results were verified with reference to in-situ drainage data from literature.The research results are helpful for mines,especially those with layered coal seams comprising tectonically deformed sub-layers and intact sub-layers,to choose appropriate gas drainage methods and develop the original drainage designs for achieving better gas drainage performance.展开更多
基金the financial support from the National Natural Science Foundation of China(Nos.52104210,52174217,and 51874294)the Natural Science Foundation of Shanxi Province(No.20210302124350)Project Funded by China Postdoctoral Science Foundation(No.2022M710595).
文摘To enhance gas drainage in the soft and hard interbedded(SHI)coal seam,it’s necessary to unload the insitu stress and improve its gas migration performance.In this research,a directional hydraulic flushing(DHF)technology was carried out.The stress-unloading and gas migration improvement mechanism was analyzed through numerical simulation,and systematic engineering tests were conducted to verify the gas drainage effect.The results show that the improvement of gas migration performance in the SHI coal seam is caused by a combined effect of seepage-improving and diffusion-improving.After DHF,stress-unloading and plastic failure could be achieved both in the soft coal(SC)sublayer and in the hard coal(HC)sublayer.However,the gas diffusion capacity improves significantly in the SC sublayer,while the gas seepage capacity improves notably in the HC sublayer.Meanwhile,the stress-unloading and gas migration improvement effect improves with the flushing radius and the thickness of the SC sublayer.Besides,after adopting the DHF technology,the gas drainage effect improved markedly.The borehole number dropped by 49%,the gas drainage ratio increased from 26.0%to 48.2%,and the average coal roadway excavation speed increased from 2.4 to 5.6 m/d.
基金support by the Marine Economy Development Foundation of Guangdong Province(Grant No.GDNRC[2022]44).
文摘The actuator is a key component of the creaming tool in drilling applications.Its jet performances determine the effective reaming efficiency.In this work,a new selective reaming tool is proposed and the RNG k-εturbulence model is used to calculate its internal and externalflowfields.In particular,special attention is paid to the design of theflushing nozzle.The results show that the jet originating from theflushing nozzle has a significant influence on rock cutting and blade cooling effects.In turn,the jet performances depend on geometric structure of the creaming actuator.In this framework,a conical-cylindrical nozzle with a diameter of 7 mm is initially considered as a basis to implement a strategy to optimize the structural parameters of the reaming actuator,and improve the related side tracking reconstruction technology.
文摘BACKGROUND Vancomycin flushing syndrome(VFS),also known as red man syndrome,is an allergic reaction to vancomycin.It typically presents as a rash on the face,neck,and upper torso after intravenous administration of vancomycin.VFS is blamed on rapid intravenous infusion of vancomycin during management and rarely happens after local use.A review of the literature showed that in the last 23 years,4 such cases have been reported.Here,we add another case of VFS developed after slow local absorption of vancomycin in cement beads.CASE SUMMARY A 44-year-old male with a known case of hypertension,no history of allergies to medications,and a history of chronic osteomyelitis of the right tibia with discharging sinus over the anterolateral aspect of the leg.The pus culture grew Staphylococcus aureus,which was sensitive to clindamycin and vancomycin.The patient underwent irrigation and debridement with the placement of vancomycin cement beads made from 4 g of vancomycin powder and 40 g of polymethyl methacrylate.Three hours postoperatively,the patient developed a pruritic,erythematous,macular rash predominantly on his face,neck,chest,and lower extremities and to a lesser extent his upper extremities.A diagnosis of VFS was made and was successfully treated with cetirizine(10 mg,oral)and methylprednisolone sodium succinate(125 mg,intravenous).The patient continued to have itching with a facial rash for 12 h with gradual improvement.A decision was made to not remove the beads as the patient continued to improve.Gradually,the rash disappeared after 96 h with no further sequela.CONCLUSION VFS can occur not only after rapid intravenous injection of vancomycin but also with local release,as in our case.As orthopaedic surgeons routinely use vancomycin with polymethyl methacrylate in chronic osteomyelitis and revision arthroplasty,they should be aware of such a complication occurring.
文摘Field experiments were carried out at the National Research Center farm, Nubaria area, Behura Governorate, Egypt, to study the effect of nitrogen source, flushing schedule and irrigation method on emitter clogging. Peanut Giza 5 variety (Arachishy pogaea L.) was planted in sandy soil during two successive growing seasons (2010-2011) in the 1st week of May and harvested after 130 days. Treatments used are: 1) two irrigation methods: surface drip irrigation and sub-surface drip irrigation (SDI;SSDI), 2) nitrogen source (NS):NH4NO3, (NH4)2SO4 and Ca(NO3)2 (NS1, NS2 and NS3) and 3) flushing number (FL) 0, 1 and 4 (FL1, FL2;FL3). The experiments design was split-split plot and three replicates were used. Data obtained were subjected to statistical analysis. The main effects of treatments used on clogging per cent could be written the following ascending orders: SDI 3 2 1, NS1 2 3. Concerning the 1st interaction the following ascending orders denote their effects on clogging percent: SDI × FL3 2 1, SDI × NS1 2 3, SSDI × FL3 2 1, SSDI × NS1 2 3, FL1 × NS1 1 × NS2 1 × NS3, FL2 × NS1 2 × NS2 2 × NS3 and FL3 × NS1 3 × NS2 3 × NS3. The differences between any two treatments and/or any two interactions in clogging percent were significant at the 5% level. The effect of the 2nd interaction on clogging percent was significant at the 5% level. The maximum value of clogging (20.18%) and the lowest one (3.9%) were archived in the interactions: SSDI × FL1 × NS3 and SDI × FL3 × NS1, respectively.
基金This project was supported by a grantfrom Hubei Scienceand Technology Comm ittee (No.96 1P190 1)
文摘The activity of matrix metalloproteinases (MMPs) in the uterine flushing and endometrial tissue of normal adult women wearing FCu IUD (fixed Cu IUD) or FICu IUD (indomethacin releasing FCu IUD) was observed by using zymography on SDS PAGE containing gelatin. The results showed that the activity and kinds of MMPs in FCu IUD group were increased significantly as compared with themselves before being inserted FCu IUD. However, compared with the FCu IUD group, the activity of some kinds of MMPs in the FICu IUD group was decreased significantly. These data suggest that IUD can enhance the activity of MMPs in human endometrium, intermediated by prostaglandins, and MMPs may have relation to IUD induced menorrhagia and indomethacin reduces IUD induced menorrhagia by partly inhibiting MMPs synthesis.
文摘This paper analyzes the situation at the Iffezheim barrage along the fiver Rhine.Since the start of operation in 1977 several million cubic meters of very fine sediment have been deposited in the reservoir.Be- cause of stability problems and reduced safety in the event of flooding,an extraction of the material is neces- sary.Chemical assays showed a high concentration of HCB.Therefore flushing without any additional measures is not appropriate.In order to leam more about the morphological and chemical in...
文摘Objective: To discuss the effect of vacuum sealing drainage combined with intermittent insulin flushing on the expression of inflammatory factors and apoptosis factors in diabetic lower limb ulcer wound. Methods: A total of 48 patients with diabetic lower limb ulcer who were treated in the Endocrine Department of the hospital between January 2016 and January 2017 were divided into the control group and research group by random number table, 24 cases in each group. Control group received vacuum sealing drainage, and research group received vacuum sealing drainage combined with intermittent insulin flushing. The differences in the expression of inflammatory factors and apoptosis genes in the wound exudate were compared between the two groups before and after treatment. Results: Before treatment, the differences in the inflammatory factor contents and apoptsis gene expression in wound exudate were not statistically significant between the two groups of patients. After treatment, inflammatory factors IL-6, IL-1β, TNF-α, CRP, VCAM-1 and FGF2 contents in wound exudate of research group were lower than those of control group;apoptosis gene Bcl-2 mRNA expression in wound exudate of research group was higher than that of control group while Bax, p53 and Fas mRNA expression were lower than those of control group. Conclusions: Vacuum sealing drainage combined with intermittent insulin flushing can effectively reduce the local inflammatory response and promote the granulation cell proliferation in wound of patients with diabetic lower limb ulcer.
基金financially supported by the National Natural Science Foundation of China(41877240)National Key Research and Development Program of China(2018YFC1802300)Scientific Research Foundation of Graduate School of Southeast University(YBPY2154).
文摘In-site soil flushing and aeration are the typical synergetic remediation technology for contaminated sites.The surfactant present in flushing solutions is bound to affect the aeration efficiency.The purpose of this study is to evaluate the effect of surfactant frequently used in soil flushing on the oxygen mass transfer in micro-nano-bubble(MNB)aeration system.Firstly,bio-surfactants and chemical surfactants were used to investigate their effects on Sauter mean diameter of bubble(dBS),gas holdup(ε),volumetric mass-transfer coefficient(kLa)and liquid-side mass-transfer coefficient(kL)in the MNB aeration system.Then,based upon the experimental results,the Sardeing's and Fr€ossling's models were modified to describe the effect of surfactant on kL in the MNB aeration.The results showed that,for the twenty aqueous surfactant solutions,with the increase in surfactant concentration,the value of dBS,kLa and kL decreased,while the value ofεand gas-liquid interfacial area(a)increased.These phenomena were mainly attributed to the synergistic effects of immobile bubble surface and the suppression of coalescence in the surfactant solutions.In addition,with the presence of electric charge,MNBs in anionic surfactant solutions were smaller and higher in number than in non-ionic surfactant solutions.Furthermore,the accumulation of surfactant on the gaseliquid interface was more conspicuous for small MNB,so the reduction of kL in anionic surfactant solutions was larger than that in non-ionic surfactant solutions.Besides,the modified Fr€ossling's model predicted the effect of surfactant on kL in MNB aeration system with reasonable accuracy.
文摘Capella油田是哥伦比亚南部重要的稠油油田,目前处于开发生产阶段,为了油田的稳产增产,需频繁对油井进行干预。Flush by Unit具有操作简单、移动高效、作业时间缩短、安全事故少等优点,作为试点引入了Flush by Unit为该油田的浅井提供修井服务,以降低桶油成本。本文介绍了Flush by Unit的结构组成、使用范围、现场使用效果,与传统修井机相比,可以大幅降低作业时间和成本,为后续规模使用Flush by Unit进行常规修井提供了依据,也为油田的增产稳产带来了机遇。
文摘AIM: To characterize the influence of location, species and treatment upon RNA degradation in tissue samples from the gastrointestinal tract. METHODS: The intestinal samples were stored in different medium for different times under varyingconditions: different species(human and rat), varying temperature(storage on crushed ice or room temperature), time point of dissection of the submucous-mucous layer from the smooth muscle(before or after storage), different rinsing methods(rinsing with Medium, PBS, RNALater or without rinsing at all) and different regions of the gut(proximal and distal small intestine, caecum, colon and rectum). The total RNA from different parts of the gut(rat: proximal and distal small intestine, caecum, colon and rectum, human: colon and rectum) and individual gut layers(muscle and submucosal/mucosal) was extracted. The quality of the RNA was assessed by micro capillary electrophoresis. The RNA quality was expressed by the RNA integrity number which is calculated from the relative height and area of the 18 S and 28 S RNA peaks. From rat distal small intestine q PCR was performed for neuronal and glial markers.RESULTS: RNA obtained from smooth muscle tissue is much longer stable than those from submucosal/mucosal tissue. At RT muscle RNA degrades after one day, on ice it is stable at least three days. Cleaning and separation of gut layers before storage and use of RNALater, maintains the stability of muscle RNA at RT for much longer periods. Different parts of the gut show varying degradation periods. RNA obtained from the submucosal/mucosal layer always showed a much worse amplification rate than RNA from muscle tissue. In general RNA harvested from rat tissue, either smooth muscle layer or submucosal/mucosal layer is much longer stable than RNA from human gut tissue, and RNA obtained from smooth muscle tissue shows an increased stability compared to RNA from submucosal/mucosal tissue. At RT muscle RNA degrades after one day, while the stability on ice lasts at least three days. Cleaning and separation of gut layers before storage and use of RNALater, maintains the stability of muscle RNA at RT for much longer periods. Different parts of the gut show varying degradation periods. The RNA from muscle and submucosal/mucosal tissue of the proximal small intestine degrades much faster than the RNA of distal small intestine, caecum or colonwith rectum. RNA obtained from the submucosal/mucosal layer always showed a much more reduced amplification rate than RNA from muscle tissue [β-Tubulin Ⅲ for muscle quantification cycle(Cp): 22.07 ± 0.25, for β-Tubulin Ⅲ submucosal/mucosal Cp: 27.42 ± 0.19].CONCLUSION: Degradation of intestinal m RNA depends on preparation and storage conditions of the tissue. Cooling, rinsing and separating of intestinal tissue reduce the degradation of m RNA.
基金Project(MSV-2013-09)supported by State Key Laboratory of Mechanical System and Vibration,China
文摘Electrical discharge milling(ED-milling) can be a good choice for titanium alloys machining and it was proven that its machining efficiency can be improved to compete with mechanical cutting. In order to improve energy utilization efficiency of ED-milling process, unstable arc discharge and stable arc discharge combined with normal discharge were implemented for material removal by adjusting servo control strategy. The influence of electrode rotating speed and dielectric flushing pressure on machining performance was investigated by experiments. It was found that the rotating of electrode could move the position of discharge plasma channel, and high pressure flushing could wash melted debris out the discharge gap effectively. Both electrode rotating motion and high pressure flushing are contributed to the improvement of machining efficiency.
基金supported by funding from Fapemig–Fundacao de AmparoàPesquisa de MG,CNPq–Conselho Nacional de Desenvolvimento Científico e Tecnológico and Capes–Coordenacao de Aperfeicoamento de Pessoal de Nível Superior
文摘Background: Nutrition is one of the most important factors that affect animal performance, and it therefore also impacts on financial results in beef systems. In this way, finding the best strategy for feeding supplements is of paramount importance. Aiming to evaluate the effect of supplement feeding strategies for beef cows in the last third of gestation, two experiments were conducted. In Experiment 1, 35 pregnant Nellore cows were assigned to a completely randomized design with four treatments: control, which received no supplement; supplementation for the last 30 d of gestation(30-d; 3.0 kg/d); supplementation for the last 60 d of gestation(60-d; 1.5 kg/d); or supplementation for the last 90 d of gestation(90-d; 1.0 kg/d). All supplemented treatments received the same total amount of supplement throughout the experiment: 90 kg(20% of crude protein). A second experiment(Experiment 2) was delineated to evaluate the effects of the amounts offered in Experiment 1 on intake and metabolism. Four multiparous pregnant Nellore cows were assigned to a 4 × 4 Latin square design, with periods of15 d each.Results: There was a linear effect of the number of days of supplementation on calving body weight(BW; P < 0.05)and a quadratic effect on BW change from parturition to d 31 post-calving(P < 0.05), with cows on the 60-d strategy losing less BW post-calving. No difference was found in offspring birth BW(P > 0.10). A significant linear effect on interval from parturition to conception(P < 0.05) was observed, with the highest calving to conception interval being observed in the 90-d strategy. The level of supplementation did not affect forage intake or neutral detergent fiber digestibility(P > 0.10). Nitrogen excreted through urine tended to increase linearly with the level of supplementation(P < 0.10).Conclusion: Providing 1.5 kg of supplement during the last 60 d of gestation improves cow performance after calving, reducing the magnitude of BW lost, and reduces the number of days from calving to re-conception in the following breeding season compared to the usually recommended period of supplementation of 90 d pre-partum.
文摘Conductive or send-conductive powder added into dielectric fluid can obviously lower the roughness of workpiece. With a mathematical ed developed to show the movement of dielectric fluid and powder particles in the gap flow field and a numerical algorithm proposed for the model, the distribution of powder particle conentration in the gap flow field is simulated with different jets for EDM.
基金National Science Foundation of China(Nos.51904311,51874314)Beijing Municipal Natural Science Foundation(8194072)+1 种基金Fundamental Research Funds for the Central Universities(2019QY02)State Key Laboratory Cultivation Base for Gas Geology and Gas Control(Henan Polytechnic University)(WS2019A04).
文摘High concentration and large flow flux of gas drainage from underground coal seams is the precondition of reducing emission and large-scale use of gas.However,the layered occurrence of coal seams with tectonically deformed sub-layers and intact sub-layers makes it difficult to effectively drain gas through commonly designed boreholes.In this study,the gas drainage performance in coal seams with different combinations of tectonically deformed sub-layers and intact sub-layers was numerically analyzed.The analysis results show that the gas drainage curve changes from a single-stage line to a dual-stage curve as the permeability ratios of Zone II(kII)and Zone I(kI)increase,raising the difficulty in gas drainage.Furthermore,a dual-system pressure decay model based on the first-order kinetic model was developed to describe the dual-stage characteristics of pressure decay curves with different permeability ratios.In the end,the simulation results were verified with reference to in-situ drainage data from literature.The research results are helpful for mines,especially those with layered coal seams comprising tectonically deformed sub-layers and intact sub-layers,to choose appropriate gas drainage methods and develop the original drainage designs for achieving better gas drainage performance.
