Background:Lotus root polysaccharide is a natural antioxidant.As a new anticancer drug,it has anti-proliferation and pro-apoptotic effects in a variety of tumour cells,but its effect on ovarian cancer is not clear.In ...Background:Lotus root polysaccharide is a natural antioxidant.As a new anticancer drug,it has anti-proliferation and pro-apoptotic effects in a variety of tumour cells,but its effect on ovarian cancer is not clear.In study,we attempted to elucidate the role and mechanism of lotus root polysaccharide in SKOV3 cells.Methods:In this study,the effect of lotus root polysaccharide on mRNA of SKVO3 cells was analyzed by RNA-seq,and verified by Western blot,flow cytometry,fluorescence detection and other techniques.Results:The results showed that lotus root polysaccharide could inhibit the proliferation of ovarian cancer cells.Then,a change in gene expression was found by RNA-seq.In the mRNA(differentially expressed mRNA)with these differences,significant changes in the cell cycle were found by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis.Subsequently,the proportion of cells in S phase decreases and G2/M phase increases,as seen with propidium iodide staining.Gene Set Enrichment Analysis showed inhibition of the cell cycle,and the gene and protein expression of CDK1,CCNA1 and CCNB1 were inhibited.Conclusion:Our results show that lotus root polysaccharide can inhibit the growth of SKOV3 cells in vitro by blocking the cell cycle at the G2/M phase,which reveals the potential of lotus root polysaccharide in the treatment of ovarian cancer.展开更多
Marketing channels are an important part of the circulation of agricultural products.With the development of China s agricultural industry,the marketing channels of agricultural products are continuously innovated and...Marketing channels are an important part of the circulation of agricultural products.With the development of China s agricultural industry,the marketing channels of agricultural products are continuously innovated and optimized.Taking Liujun Lotus Root as an example,on the basis of studying the existing model of marketing channels of agricultural products,this paper analyzes the problems in the existing marketing channels of Liujun Lotus Root,and puts forward relevant strategies.展开更多
[Objective] The experiment aimed to study the growth characteristics of hydroponic bowl lotus. [Method] The lotus variety Hongxia was chosen as the experimental material. Two treatments, hydroponics and soil culture w...[Objective] The experiment aimed to study the growth characteristics of hydroponic bowl lotus. [Method] The lotus variety Hongxia was chosen as the experimental material. Two treatments, hydroponics and soil culture were set to measure their photosynthetic indices, chlorophyll content and root vigor, and to observe their leaf tissue structure and stomatal characteristics. [Result] The findings indicated that there are no differences in the leaf physiological indices between bowl lotus under hydroponics and soil culture, while the leaf stomata of hydroponic bowl lotus is bigger and its amount is larger than that of soil-culture bowl lotus. At the same time, the ratio of the palisade tissue thickness to spongy tissue thickness is small,and its leaf tissue structure is loose. The root vigor of hydroponic bowl lotus reached its summit earlier, then began to drop. Whereas, the root activity of soil-culture lotus sustained increasing, with vigorous growth. [Conclusion] Therefore, it indicated that hydroponic bowl lotus can adapt to the aquatic-culture environment well and quickly, meanwhile, it also enters into its aging period quickly and its growth cycle gets shorter.展开更多
Lotus root-redswamp crayfish integrated farming is a kind of ecologicalgrow mode on the basis of paddy field, which is an innovated mode for agricultural structure adjustment and special agriculture with high efficien...Lotus root-redswamp crayfish integrated farming is a kind of ecologicalgrow mode on the basis of paddy field, which is an innovated mode for agricultural structure adjustment and special agriculture with high efficiency. In order to regulate producing technology of this mode, the essay puts forward some technical require- ments, stressing on cultivar selection, field works, farming technology and product quality, which aims at providing technical support for agricultural production.展开更多
Objective] This study almed to optimize the processing technoIogy of compound beverage of Iotus root and Iemon. [Method] The Iotus root and Iemon were seIected as raw materials for preparing compound beverage. The Iot...Objective] This study almed to optimize the processing technoIogy of compound beverage of Iotus root and Iemon. [Method] The Iotus root and Iemon were seIected as raw materials for preparing compound beverage. The Iotus root and Iemon were juiced respectiveIy. SubsequentIy, their juices were mixed together with a series of proportions so as to expIore a rational processing ratio of Iotus root and Iemon. [Result] The resuIts showed that the browning of Iotus root juice couId be effectiveIy prevented when the Iotus root juice was heated for 9 min and added with 0.8% citric acid. The optimized formuIa of compound beverage of Iotus root and Iemon was as foI ows： Iotus root juice to Iemon juice ratio, 5：1; addition amount of white granuIated sugar, 12%; addition amount of stabiIizer （CMC-Na）, 0.15%. Af-ter moduIation, homogenization, steriIization and other processes, the compound beverage of Iotus root and Iemon was prepared. [Conclusion] The compound bever-age, prepared by optimized technoIogy and formuIa, was characterized by a IittIe of yeI ow coIor, fuI of Iotus root and Iemon fIavors, rich nutrients, smooth taste with sweet and sour, as weI as good stabiIity.展开更多
Bahu lotus root is a specialty of Hedong District,Linyi City,Shandong Province.It was recognized as a national product of geographical indication in 2011.This article describes the specific production area,unique prod...Bahu lotus root is a specialty of Hedong District,Linyi City,Shandong Province.It was recognized as a national product of geographical indication in 2011.This article describes the specific production area,unique production environment,rich cultural history and unique quality of Bahu lotus root,and summarizes its unique production from four aspects:selection of origin,selection of variety,production management and timely harvest,aiming to maintain the brand of Bahu lotus root to the greatest extent and further enhance the brand value and influence.展开更多
基金This work was supported by High Level Talents Research Fund Project of Qingdao Agricultural University in China(No.1120043).
文摘Background:Lotus root polysaccharide is a natural antioxidant.As a new anticancer drug,it has anti-proliferation and pro-apoptotic effects in a variety of tumour cells,but its effect on ovarian cancer is not clear.In study,we attempted to elucidate the role and mechanism of lotus root polysaccharide in SKOV3 cells.Methods:In this study,the effect of lotus root polysaccharide on mRNA of SKVO3 cells was analyzed by RNA-seq,and verified by Western blot,flow cytometry,fluorescence detection and other techniques.Results:The results showed that lotus root polysaccharide could inhibit the proliferation of ovarian cancer cells.Then,a change in gene expression was found by RNA-seq.In the mRNA(differentially expressed mRNA)with these differences,significant changes in the cell cycle were found by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis.Subsequently,the proportion of cells in S phase decreases and G2/M phase increases,as seen with propidium iodide staining.Gene Set Enrichment Analysis showed inhibition of the cell cycle,and the gene and protein expression of CDK1,CCNA1 and CCNB1 were inhibited.Conclusion:Our results show that lotus root polysaccharide can inhibit the growth of SKOV3 cells in vitro by blocking the cell cycle at the G2/M phase,which reveals the potential of lotus root polysaccharide in the treatment of ovarian cancer.
文摘Marketing channels are an important part of the circulation of agricultural products.With the development of China s agricultural industry,the marketing channels of agricultural products are continuously innovated and optimized.Taking Liujun Lotus Root as an example,on the basis of studying the existing model of marketing channels of agricultural products,this paper analyzes the problems in the existing marketing channels of Liujun Lotus Root,and puts forward relevant strategies.
基金Supported by Key Scientific and Technological Project of Henan Province(072102150001)~~
文摘[Objective] The experiment aimed to study the growth characteristics of hydroponic bowl lotus. [Method] The lotus variety Hongxia was chosen as the experimental material. Two treatments, hydroponics and soil culture were set to measure their photosynthetic indices, chlorophyll content and root vigor, and to observe their leaf tissue structure and stomatal characteristics. [Result] The findings indicated that there are no differences in the leaf physiological indices between bowl lotus under hydroponics and soil culture, while the leaf stomata of hydroponic bowl lotus is bigger and its amount is larger than that of soil-culture bowl lotus. At the same time, the ratio of the palisade tissue thickness to spongy tissue thickness is small,and its leaf tissue structure is loose. The root vigor of hydroponic bowl lotus reached its summit earlier, then began to drop. Whereas, the root activity of soil-culture lotus sustained increasing, with vigorous growth. [Conclusion] Therefore, it indicated that hydroponic bowl lotus can adapt to the aquatic-culture environment well and quickly, meanwhile, it also enters into its aging period quickly and its growth cycle gets shorter.
基金Supported by the Key Agricultural Supporting Program of Jiangsu Province(BE2014360)~~
文摘Lotus root-redswamp crayfish integrated farming is a kind of ecologicalgrow mode on the basis of paddy field, which is an innovated mode for agricultural structure adjustment and special agriculture with high efficiency. In order to regulate producing technology of this mode, the essay puts forward some technical require- ments, stressing on cultivar selection, field works, farming technology and product quality, which aims at providing technical support for agricultural production.
文摘Objective] This study almed to optimize the processing technoIogy of compound beverage of Iotus root and Iemon. [Method] The Iotus root and Iemon were seIected as raw materials for preparing compound beverage. The Iotus root and Iemon were juiced respectiveIy. SubsequentIy, their juices were mixed together with a series of proportions so as to expIore a rational processing ratio of Iotus root and Iemon. [Result] The resuIts showed that the browning of Iotus root juice couId be effectiveIy prevented when the Iotus root juice was heated for 9 min and added with 0.8% citric acid. The optimized formuIa of compound beverage of Iotus root and Iemon was as foI ows： Iotus root juice to Iemon juice ratio, 5：1; addition amount of white granuIated sugar, 12%; addition amount of stabiIizer （CMC-Na）, 0.15%. Af-ter moduIation, homogenization, steriIization and other processes, the compound beverage of Iotus root and Iemon was prepared. [Conclusion] The compound bever-age, prepared by optimized technoIogy and formuIa, was characterized by a IittIe of yeI ow coIor, fuI of Iotus root and Iemon fIavors, rich nutrients, smooth taste with sweet and sour, as weI as good stabiIity.
基金Supported by Subsidy Project for Reform and Construction of Grassroots Agricultural Technology Extension System(Lu Nong Cai Zi[2017]77).
文摘Bahu lotus root is a specialty of Hedong District,Linyi City,Shandong Province.It was recognized as a national product of geographical indication in 2011.This article describes the specific production area,unique production environment,rich cultural history and unique quality of Bahu lotus root,and summarizes its unique production from four aspects:selection of origin,selection of variety,production management and timely harvest,aiming to maintain the brand of Bahu lotus root to the greatest extent and further enhance the brand value and influence.
