Aim:Protective effects of aqueous extract of Amaranthus hybridus against afl atoxin B1(AFB_(1))and/or fumonisin B1(FB_(1))on the H4IIE-luc cell line were determined by use of the methyl thiazol tetrazolium viability a...Aim:Protective effects of aqueous extract of Amaranthus hybridus against afl atoxin B1(AFB_(1))and/or fumonisin B1(FB_(1))on the H4IIE-luc cell line were determined by use of the methyl thiazol tetrazolium viability assay and disruption of DNA integrity.Methods:H4IIE-luc cells were incubated with different concentrations of AFB_(1) and/or FB_(1) for 24 and 48 h with or without aqueous extract of A.hybridus.Results:AFB_(1) decreased the viability of cells after 24 and 48 h of exposure.EC_(50)values for AFB_(1) were 10.5 and 1.8μmol/L for the two periods,respectively.When the 48 h exposure to mycotoxin repeated with a pre-treatment of 20 and 40μg/mL extract of A.hybridus,the EC_(50)changed to 3.88 and 7.67μmol/L,respectively.H4IIE-luc cells exposed to FB_(1) for 24 h responded more than those incubated for 48 h.Cells treated with a combination of AFB_(1) and FB_(1) were less viable with a signifi cant decrease in the greater concentration.The mixture of AFB_(1) and FB_(1) resulted in a signifi cant threat to H4IIE-luc as indicated by the absence or appearance of new bands in random amplifi ed polymorphic DNA analysis,which demonstrated damage to DNA.The protective effects were probably due to greater content of total phenolics,carotenoids,β-carotene,folic-,linolenic-,linoleic and palmitic acids,as well as calcium,magnesium,iron,zinc,and selenium observed in the extract.Conclusion:Exposure to 40μg/mL of extract of A.hybridus protected cells from damage to DNA by stabilizing DNA.展开更多
The peroxisomal matrix oxidase,catalase and peroxidase are imported peroxisomes through the shuttling receptors,which regulates the cellular oxidative homeostasis and function.Here,we report that PTS1 shuttling recept...The peroxisomal matrix oxidase,catalase and peroxidase are imported peroxisomes through the shuttling receptors,which regulates the cellular oxidative homeostasis and function.Here,we report that PTS1 shuttling receptor FvPex5 is involved in the localization of PTS1,utilization of carbon sources and lipids,elimination ROS,cell wall stress,conidiation,fumonisin B_(1)(FB_(1))production,and virulence in maize pathogen Fusarium verticillioides.Significantly,differential expression of PTS1-,PTS2-,PEX-and FB_(1)toxin-related genes in wild type andΔFvpex5 mutant were examined by RNA-Seq analyses and confirmed by RT-PCR assay.In addition,different expression of PTS1 and PTS2 genes of theΔFvpex5 mutant were enriched in diverse biochemical pathways,such as carbon metabolism,nitrogen metabolism,lipid metabolism and the oxidation balance by combining GO and KEGG annotations.Overall,we showed that FvPex5 is involved in the regulation of genes associated with PTS,thereby affecting the oxidation balance,FB_(1)and virulence in F.verticillioides.The results help to clarify the functional divergence of Pex5 orthologs,and may provide a possible target for controlling F.verticillioides infections and FB_(1)biosynthesis.展开更多
基金The Morogo Research Program gratefully acknowledges the National Research Foundation of South Africa(Focus Area Grant FA2004050600064)National Research Center,Cairo,Egypt Project No.10070112 for financial support of this study.Prof.Giesy was supported by the Canada Research Chair program,a Visiting Distinguished Professorship in the Department of Biology and Chemistry and State Key Laboratory in Marine Pollution,City University of Hong Kong,the 2012“High Level Foreign Experts”(No.GDT20143200016)program,funded by the State Administration of Foreign Experts Affairs,the P.R.China to Nanjing University and the Einstein Professor Program of the Chinese Academy of Sciences.
文摘Aim:Protective effects of aqueous extract of Amaranthus hybridus against afl atoxin B1(AFB_(1))and/or fumonisin B1(FB_(1))on the H4IIE-luc cell line were determined by use of the methyl thiazol tetrazolium viability assay and disruption of DNA integrity.Methods:H4IIE-luc cells were incubated with different concentrations of AFB_(1) and/or FB_(1) for 24 and 48 h with or without aqueous extract of A.hybridus.Results:AFB_(1) decreased the viability of cells after 24 and 48 h of exposure.EC_(50)values for AFB_(1) were 10.5 and 1.8μmol/L for the two periods,respectively.When the 48 h exposure to mycotoxin repeated with a pre-treatment of 20 and 40μg/mL extract of A.hybridus,the EC_(50)changed to 3.88 and 7.67μmol/L,respectively.H4IIE-luc cells exposed to FB_(1) for 24 h responded more than those incubated for 48 h.Cells treated with a combination of AFB_(1) and FB_(1) were less viable with a signifi cant decrease in the greater concentration.The mixture of AFB_(1) and FB_(1) resulted in a signifi cant threat to H4IIE-luc as indicated by the absence or appearance of new bands in random amplifi ed polymorphic DNA analysis,which demonstrated damage to DNA.The protective effects were probably due to greater content of total phenolics,carotenoids,β-carotene,folic-,linolenic-,linoleic and palmitic acids,as well as calcium,magnesium,iron,zinc,and selenium observed in the extract.Conclusion:Exposure to 40μg/mL of extract of A.hybridus protected cells from damage to DNA by stabilizing DNA.
基金supported by the National Natural Science Foundation of China(31601599)the Science and Technology Innovation Funding of Fujian Agriculture and Forestry University,China(CXZX2020044A)。
文摘The peroxisomal matrix oxidase,catalase and peroxidase are imported peroxisomes through the shuttling receptors,which regulates the cellular oxidative homeostasis and function.Here,we report that PTS1 shuttling receptor FvPex5 is involved in the localization of PTS1,utilization of carbon sources and lipids,elimination ROS,cell wall stress,conidiation,fumonisin B_(1)(FB_(1))production,and virulence in maize pathogen Fusarium verticillioides.Significantly,differential expression of PTS1-,PTS2-,PEX-and FB_(1)toxin-related genes in wild type andΔFvpex5 mutant were examined by RNA-Seq analyses and confirmed by RT-PCR assay.In addition,different expression of PTS1 and PTS2 genes of theΔFvpex5 mutant were enriched in diverse biochemical pathways,such as carbon metabolism,nitrogen metabolism,lipid metabolism and the oxidation balance by combining GO and KEGG annotations.Overall,we showed that FvPex5 is involved in the regulation of genes associated with PTS,thereby affecting the oxidation balance,FB_(1)and virulence in F.verticillioides.The results help to clarify the functional divergence of Pex5 orthologs,and may provide a possible target for controlling F.verticillioides infections and FB_(1)biosynthesis.
