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Combined Effect of Temperature and Cadmium on Molecular Responses of Hsp70 and P-gp Genes in Crassostrea gigas
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作者 HU Fanguang SUN Ming +3 位作者 LIU Guangbin JING Yuanyuan CHEN Qun ZHANG Tianwen 《Journal of Ocean University of China》 SCIE CAS CSCD 2024年第4期1115-1120,共6页
To evaluate the combined effect of temperature and cadmium on the molecular responses of heat shock protein 70(hsp70)and P-glycoprotein(P-gp)in mantle,digestive gland and gills of Crassostrea gigas,oysters were expose... To evaluate the combined effect of temperature and cadmium on the molecular responses of heat shock protein 70(hsp70)and P-glycoprotein(P-gp)in mantle,digestive gland and gills of Crassostrea gigas,oysters were exposed to combinations of five temperature levels(10,15,20,25,and 30℃)and 10μg L^(-1)cadmium for 21 days.Oysters were sampled for mRNA quantification by qPCR,and the results showed that the P-gp gene expression changed significantly after treatment at different temperatures and different treatment times.The P-gp gene expression was the highest in the digestive gland.Compared with the control group,the P-gp gene expression in cadmium treatment groups at all the different temperatures were significantly higher than the control group.The control oysters(kept at 10℃during the whole experiment without cadmium)expressed low levels of hsp70,but the groups treated with cadmium displayed somewhat higher levels.The present study demonstrated hsp70 and P-gp played an important role in the detoxification of Cd in C.gigas,and confirmed temperature should be considered for the assessment of Cd-induced toxicity in oysters. 展开更多
关键词 temperature CADMIUM P-GP HSP70 Crassostrea gigas
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Transcriptome Analysis of Heterosis in Survival in the Hybrid Progenies of‘Haida No.1’and Orange-Shelled Lines of the Pacific Oyster Crassostrea gigas
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作者 YANG Hang LI Qi 《Journal of Ocean University of China》 CAS CSCD 2024年第1期199-208,共10页
Heterosis has been exploited to enhance the yield and adaptability in various shellfish species;however,the molecular basis of it remains unclear.The Pacific oyster Crassostrea gigas is one of the most economically im... Heterosis has been exploited to enhance the yield and adaptability in various shellfish species;however,the molecular basis of it remains unclear.The Pacific oyster Crassostrea gigas is one of the most economically important aquaculture species,and its productive traits can be improved by hybridization.Here,an intraspecific cross between orange shell(O,10th generation)and‘Haida No.1’(H,13th generation)of C.gigas was performed to assess the heterosis of survival trait.Survival rates of hybrid family(OH)and inbred families(HH and OO)were compared at larval stage,and eyed-pediveliger larvae of three families were subjected to transcriptome analysis.The analysis results of best-parent heterosis and mid-parent heterosis showed that the hybrid family exhi-bited a high heterosis in survival relative to the parental families.The OH-M(OH vs.OO)and OH-P(OH vs.HH)had 425 and 512 dif-ferentially expressed genes(DEGs),respectively.Functional enrichment analysis of these DEGs revealed that the significantly enrich-ed genes function in virion binding,C-type lectin receptor signaling pathway,cellular defense response and other immune-related pro-cesses,which involves perlucin-like protein,CD209 antigen-like protein,ZNFX1,caspase-3 and acan genes.These differentially ex-pressed genes in OH-M and OH-P,together with the immune-related processes mentioned above may play an important role in the larval survival of C.gigas.In addition,three genes(CYP450,fucolectin and perlucin-like)are associated with the orange shell and low survival of maternal oyster OO.These findings provide support for the application of hybrid with superior survival and will facilitate the understanding of heterosis formation in the Pacific oyster. 展开更多
关键词 Crassostrea gigas survival rate HETEROSIS TRANSCRIPTOME
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Dietary Supplementation of β-Carotene Reveals miRNAs Involved in the Regulation of Carotenoid Metabolism in Crassostrea gigas
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作者 SUN Dongfang WAN Sai +2 位作者 YU Hong KONG Lingfeng LI Qi 《Journal of Ocean University of China》 SCIE CAS CSCD 2024年第4期1045-1053,共9页
Carotenoids play crucial physiological roles in animals.A comprehensive investigation into the mechanism of carotenoid metabolism in oysters will establish a theoretical foundation for further development of its carot... Carotenoids play crucial physiological roles in animals.A comprehensive investigation into the mechanism of carotenoid metabolism in oysters will establish a theoretical foundation for further development of its carotenoid-rich traits.However,the information on the function of miRNA in β-carotene metabolism in oysters is limited.To elucidate the mechanisms underlying miRNA regulation of carotenoid metabolism in oysters,we compared the expressions of miRNA in digestive gland tissues of Pacific oyster(Crassostrea gigas)fed with aβ-carotene supplemented diet and a normal diet,respectively.A total of 690 candidate miRNAs in the Pacific oyster digestive gland tissues were identified,including 590 known miRNAs and 111 unknown miRNAs.Three differentially expressed miRNAs were obtained in the carotenoid-fed and normal groups,associated to 137 differentially expressed target genes.Moreover,the GO enrichment analysis revealed that the differentially expressed target genes were mainly involved in transmembrane transport activity.KEGG enrichment showed that the differentially expressed target genes were involved in ABC transport.Analysis of the mRNA-miRNA network revealed that novel0025 played a central role in carotenoid metabolism,and it was negatively correlated with the expression of 46 mRNAs.In addition,down-regulated expression of novel0025 upregulated the expression of the lipoprotein gene LOC105342186,suggesting a potential regulatory role in carotenoid metabolism.Our results provide useful information for elucidating the miRNA regulation mechanism during carotenoids metabolism in the Pacific oyster. 展开更多
关键词 Crassostrea gigas Β-CAROTENE microRNA carotenoid metabolism
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Pacific oyster(Crassostrea gigas)shell growth duration in a year in Bohai Bay and implication for its carbon sink potential
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作者 Wen-yu Wang Chang-fu Fan +2 位作者 Zhao-jun Song Hong Wang Fu Wang 《China Geology》 CAS CSCD 2024年第4期653-660,共8页
Oyster is a bivalve mollusk widely distributed in estuarine and shallow sea environments.Its growth and burial process is a carbon sequestration and storage process.Oyster shell may stop growing due to suffer from fre... Oyster is a bivalve mollusk widely distributed in estuarine and shallow sea environments.Its growth and burial process is a carbon sequestration and storage process.Oyster shell may stop growing due to suffer from freeze shock during the winter season within a temperate climate,therefore,in order to study the carbon sequestration capacity of oysters we need to know the water temperature at which the shell suffer from winter freeze shock.This study examinesδ^(18)O profiles across consecutive micro-growth layers found in three modern Pacific oyster shells from the northwest coast of Bohai Bay.A total of 165 oxygen isotope values from sequential samples of their left shells showed periodically varying values,and the variation fluctuation of oxygen isotope values was 4.97‰on average.According to the variation range of the oxygen isotope value of the shell,combined with the sea surface temperature and the sea surface salinity data of the water in which the oysters grew,the water temperature that suffer from winter freeze shock and stops or retards the growth of Pacific oysters in Bohai Bay is about 8.3℃,and the corresponding period is from December to March of the following year.The calcification time of oysters within one year is nearly a month longer than previously thought,therefore,its carbon sink potential is also improved. 展开更多
关键词 Crassostrea gigas Winter freeze shock Oxygen isotope Bohai Bay Carbon sequestration and storage Environmental geological survey
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长牡蛎(Crassostrea gigas)野生与选育群体的微卫星遗传多样性分析
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作者 李妍 姚健涛 +6 位作者 张恩烁 孙泽轩 孙国华 李彬 杨建敏 冯艳微 王卫军 《海洋与湖沼》 CAS CSCD 北大核心 2024年第2期462-470,共9页
为了评估长牡蛎(Crassostrea gigas)2个壳长性状(掌心形)快速生长选育群体(LY2-K4、LY2-K7)、1个壳高性状(速生型)快速生长选育群体(LY2-K11)和6个野生群体(QHD、LS、HD、ZH、WD、KTD)的遗传多样性和遗传结构,用21对多态性丰富的微卫星... 为了评估长牡蛎(Crassostrea gigas)2个壳长性状(掌心形)快速生长选育群体(LY2-K4、LY2-K7)、1个壳高性状(速生型)快速生长选育群体(LY2-K11)和6个野生群体(QHD、LS、HD、ZH、WD、KTD)的遗传多样性和遗传结构,用21对多态性丰富的微卫星引物对9个长牡蛎群体的269个个体进行了遗传分析。结果显示:21个微卫星位点共检测出了460个等位基因(Na),平均等位基因数为21.905;21个微卫星位点的多态信息含量(PIC)均大于0.5,具有高度遗传多态性;选育群体LY2-K11的遗传多样性最低(Na=13,I=2.128,He=0.831,PIC=0.825),野生群体KTD的遗传多样性最高(Na=29,I=3.112,He=0.941,PIC=0.938);189个群体位点组合有66%偏离哈代-温伯格平衡,表明这些群体存在一定程度的杂合子缺失;9个群体间的遗传分化指数(F_(st))为0.012~0.064,处于较低的遗传分化水平;AMOVA分析显示遗传变异主要来自于个体内;PCoA分析结果与UPGMA聚类树一致,LY2-K11群体单独聚为一类,QHD和HD群体聚为一类,其他6个群体聚为一类。综上所述,长牡蛎3个选育群体和6个野生群体遗传多样性均较高,遗传分化水平较低;选育群体LY2-K11多样性略有下降,选育过程中应保证亲本的数量及质量,防止因近交衰退造成遗传多样性降低,苗种抗逆性变差。该结果将为长牡蛎新品种的选育和野生种质资源的保护提供科学指导。 展开更多
关键词 长牡蛎(Crassostrea gigas) 选育群体 野生群体 微卫星 遗传多样性
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Pigment Distribution and Secretion in the Mantle of the Pacific Oyster(Crassostrea gigas) 被引量:1
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作者 ZHU Yijing LI Qi +1 位作者 YU Hong LIU Shikai 《Journal of Ocean University of China》 SCIE CAS CSCD 2023年第3期813-820,共8页
The color of Mollusca shells is one of the most important attributes to consumers.At the cellular level,black color is mainly from the melanin produced by melanocytes.The melanosome is a specialized membrane-bound org... The color of Mollusca shells is one of the most important attributes to consumers.At the cellular level,black color is mainly from the melanin produced by melanocytes.The melanosome is a specialized membrane-bound organelle that is involved in melanin synthesis,storage,and transportation.How the complex pigmentation process in the Crassostrea gigas is established remains an open question.The objectives of this studies are to examine the morphological characteristics of melanosomes or melanin of mantle pigmentation in the Pacific oyster,thereby investigating its contribution to shell color.The results show that pigmented granules of the mantles vary among the three lobes,and the melanosomes at different stages are enriched in distinct cargo molecules,which indicate the remarkable difference between the marginal mantle and central mantle.Examination of mantle histology reveals that the mantle margin of the oyster is characterized by three different folds,including the outer secretory,middle sensory,and inner muscular fold.Ferrous ion chelating assays against the tyrosine hydroxylase indicate that a large amount of melanin is localized in the inner surface of the middle fold.Transmission electron microscopy analyses show that the mantle edge is composed of tall columnar and cuboidal epidermal cells and some pigmented melanocytes intersperse among these cells.The numbers of melanosomes among the three lobes are different.In the inner fold and the middle fold of the mantle,some single dispersion,or aggregation of melanosomes with different degrees of melanization are found in the outer surface.Numerous melanosomes are distributed in the epithelium of the outer fold of the mantle,and mainly are at the apical microvillar surface near the lumen.However,melanosomes are occasionally observed in the central mantle,and they are relatively less.This work provides new insights into the process of melanin deposit in the mantle and shell pigmentation in C.gigas. 展开更多
关键词 Crassostrea gigas MANTLE MELANOSOME MELANIN PIGMENTATION cells
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Near-infrared spectroscopy method for rapid proximate quantitative analysis of nutrient composition in Pacific oyster Crassostrea gigas 被引量:1
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作者 Zhe LI Haigang QI +4 位作者 Ying YU Cong LIU Rihao CONG Li LI Guofan ZHANG 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2023年第1期342-351,共10页
Glycogen,amino acids,fatty acids,and other nutrient components affect the flavor and nutritional quality of oysters.Methods based on near-infrared reflectance spectroscopy(NIRS)were developed to rapidly and proximatel... Glycogen,amino acids,fatty acids,and other nutrient components affect the flavor and nutritional quality of oysters.Methods based on near-infrared reflectance spectroscopy(NIRS)were developed to rapidly and proximately determine the nutrient content of the Pacific oyster Crassostreagigas.Samples of C.gigas from 19 costal sites were freeze-dried,ground,and scanned for spectral data collection using a Fourier transform NIR spectrometer(Thermo Fisher Scientific).NIRS models of glycogen and other nutrients were established using partial least squares,multiplication scattering correction first-order derivation,and Norris smoothing.The R_(C) values of the glycogen,fatty acids,amino acids,and taurine NIRS models were 0.9678,0.9312,0.9132,and 0.8928,respectively,and the residual prediction deviation(RPD)values of these components were 3.15,2.16,3.11,and 1.59,respectively,indicating a high correlation between the predicted and observed values,and that the models can be used in practice.The models were used to evaluate the nutrient compositions of 1278 oyster samples.Glycogen content was found to be positively correlated with fatty acids and negatively correlated with amino acids.The glycogen,amino acid,and taurine levels of C.gigas cultured in the subtidal and intertidal zones were also significantly different.This study suggests that C.gigas NIRS models can be a cost-effective alternative to traditional methods for the rapid and proximate analysis of various slaughter traits and may also contribute to future genetic and breeding-related studies in Pacific oysters. 展开更多
关键词 Pacific oyster Crassostrea gigas near-infrared reflectance spectroscopy(NIRS) nutrient composition rapid determination
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Productive Traits and Triploid Rate Stability in Triploidy-Induced ‘Haida No. 2’ Strain of the Pacific Oyster, Crassostrea gigas
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作者 LI Yongguo LI Qi +1 位作者 LIU Ye XU Chengxun 《Journal of Ocean University of China》 SCIE CAS CSCD 2023年第1期229-234,共6页
In order to evaluate the effects of triploidy induction on a selected strain‘Haida No.2’of the Pacific oyster Crassostrea gigas,which is characterized with golden shell color and high growth rate,the growth,survival... In order to evaluate the effects of triploidy induction on a selected strain‘Haida No.2’of the Pacific oyster Crassostrea gigas,which is characterized with golden shell color and high growth rate,the growth,survival rate and stability of triploid rate were analyzed at different development stages in the present study.Three different conditions inhibiting the release of polar body Ⅱ or polar body Ⅰ were tested:(A)Cytochalasin-B(CB),0.5mg L^(−1) at 10min post-insemination for 15 min;(B)CB,0.5mg L^(−1)at 15 min postinsemination for 20 min;and(C)CB,0.7mg L^(−1),at 15 min post-insemination for 20 min.The triploidy induction treatments significantly reduced the D-larvae and survival rates at the larvae stage but not at the juvenile and adult stages.Triploid rate dramatically decreased at the larval stage and did not significantly change at the juvenile and adult stages.Regarding the stability of the triploid rate,there was a significant difference between the three treatment groups.Larvae from the treatment A and control groups exhibited higher growth rates in shell height than those from the other two treatment groups at day 27.Triploid juveniles and adults from the treatment A group exhibited a higher wet weight than diploids from the control group and triploids from the other treatment groups.Triploidy induction did not affect the shell color of the progeny.The results obtained in the study demonstrate that triploidy induction has the potential to be used to increase the production of C.gigas variety‘Haida No.2’without modifying its golden shell color. 展开更多
关键词 Crassostrea gigas selective strain triploidy induction survival growth triploid rate stability
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长牡蛎(Crassostrea gigas)代谢率的季节变化及其与夏季死亡关系的探讨 被引量:25
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作者 毛玉泽 周毅 +3 位作者 杨红生 袁秀堂 文海翔 王如才 《海洋与湖沼》 CAS CSCD 北大核心 2005年第5期445-451,共7页
2002年6月—2003年9月,在中国北方重要养殖海区桑沟湾,采用呼吸瓶法现场研究了两种不同规格长牡蛎(Crassostrea gigas)的耗氧率、排氨率及氧氮比的季节变化,从代谢水平探讨了长牡蛎夏季死亡的原因。结果表明,长牡蛎的耗氧率、排氨率以... 2002年6月—2003年9月,在中国北方重要养殖海区桑沟湾,采用呼吸瓶法现场研究了两种不同规格长牡蛎(Crassostrea gigas)的耗氧率、排氨率及氧氮比的季节变化,从代谢水平探讨了长牡蛎夏季死亡的原因。结果表明,长牡蛎的耗氧率、排氨率以及氧氮比均具有明显的季节变化(P<0.001)。三龄牡蛎的个体耗氧率7月份最高[2.86mgO2/(ind·h)],1月份最低[0.07mgO2/(ind·h)];个体排氨率8月份最高[6.45μmol NH4-N/(ind·h)],1月份最低[0.47μmolNH4-N/(ind·h)];一龄牡蛎个体耗氧率和排氨率的变化范围分别为0.01—1.38mgO2/(ind·h)和0.04—1.66μmol NH4-N/(ind·h)。三龄个体单位个体耗氧率、排氨率高于一龄牡蛎,而单位软体干重的耗氧率、排氨率则小于一龄个体。一龄个体的氧氮比变化范围为16.7—58.0,10月份最高,一月份最低;三龄个体的氧氮比变化范围为8.7—64.0,6—7月份较高平均为52.7,8月份产卵后迅速下降到14.7,氧氮比的剧降表明该阶段长牡蛎代谢消耗了大量的蛋白质,使其体质脆弱,可能是引起三龄牡蛎夏季大量死亡的原因之一。 展开更多
关键词 长牡蛎(Crassostrea gigas) 代谢率 夏季死亡 桑沟湾
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Genetic Variation of Wild and Hatchery Populations of the Pacific Oyster Crassostrea gigas Assessed by Microsatellite Markers 被引量:10
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作者 于红 李琪 《Journal of Genetics and Genomics》 SCIE CAS CSCD 北大核心 2007年第12期1114-1122,共9页
Microsatellite DNA technique was used to detect the genetic variation between five hatchery populations of the Pacific oyster from China and two wild populations from Japan. Seven microsatellite loci screened in this ... Microsatellite DNA technique was used to detect the genetic variation between five hatchery populations of the Pacific oyster from China and two wild populations from Japan. Seven microsatellite loci screened in this study showed high polymorphism in both hatchery and wild populations, as observed in an average number of allele per locus (19.1-29.9) and average expected heterozygosity (0.916-0.958). No significant difference in average allelic richness or expected heterozygosity was observed between Chinese hatchery populations and Japanese wild populations. Pairwise Fsr values and heterogeneity tests of allele frequencies showed significant genetic differentiation between all populations. According to the neighbor-joining tree constructed on the basis of the Dc distance, the seven populations fell into three groups showing a clear division between hatchery and wild populations, and between the northern and southern hatchery populations. Assignment tests correctry assigned high percentages (97%-100%) of individuals to their original populations and demonstrated the feasibility of microsatellite analysis for discrimination between populations. The information obtained in this study is useful for designing suitable management guidelines and selective breeding programs for the Pacific oyster in China. 展开更多
关键词 Crassostrea gigas genetic variation HATCHERY WILD MICROSATELLITE
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长牡蛎(Crassostrea gigas)EST串联重复序列的组成和分布 被引量:5
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作者 张琳琳 李莉 张国范 《海洋科学》 CAS CSCD 北大核心 2011年第4期9-14,共6页
长牡蛎(Crassostrea gigas)串联重复序列分析研究较少,为了研究其在基因组转录本的基本结构特征并为长牡蛎中遗传多样性研究中提供有益的信息,利用NCBI公共数据库中的57139条长牡蛎ESTs序列对串联重复序列结构类型、分布、丰度等进... 长牡蛎(Crassostrea gigas)串联重复序列分析研究较少,为了研究其在基因组转录本的基本结构特征并为长牡蛎中遗传多样性研究中提供有益的信息,利用NCBI公共数据库中的57139条长牡蛎ESTs序列对串联重复序列结构类型、分布、丰度等进行系统比较分析。分析结果表明:1)长牡蛎EST中共有小卫星串联重复序列8392个,在大于100bp重复类型中,162~167bp含量最高;2)长牡蛎ESTSSR含量丰富,1954个位点是EST-SSR标记开发的候选资源;3)EST-SSR重复数目和重复类型在5’UTR,CDS和3'UTR具有显著差异,CDS区承受更大的选择压力。 展开更多
关键词 长牡蛎(Crassostrea gigas) EST 串联重复序列 SSR 选择压力
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Foraging behavior of parasitoid chalcid to the essential oil from bark of Populus pseudo-simonii P. nigra and Quadraspidiotus gigas 被引量:2
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作者 迟德富 Rafael Ocete Rubio +2 位作者 严善春 张丹丹 温振宏 《Journal of Forestry Research》 SCIE CAS CSCD 2002年第4期255-259,共5页
采用四臂式嗅觉仪测定了长棒四节蚜小蜂(Pteroptrix longgiclava(Girault)(Hymenoptera:Aphelinidae))和黄胸扑虱蚜小蜂(Encarsia gigas(Tshumakova)(Hymenoptera:Aphelinidae)),对健康的小青×黑杨(Populus pseudo-simonii×... 采用四臂式嗅觉仪测定了长棒四节蚜小蜂(Pteroptrix longgiclava(Girault)(Hymenoptera:Aphelinidae))和黄胸扑虱蚜小蜂(Encarsia gigas(Tshumakova)(Hymenoptera:Aphelinidae)),对健康的小青×黑杨(Populus pseudo-simonii×P.nigra)树皮、被杨圆蚧(Quadraspidiotus gigas(Thiem & Gerneck))危害的杨树皮、杨圆蚧1齿固定若虫的虫体和介壳的挥发油的趋性反应。测定结果表明:被杨圆蚧危害后的小青×黑杨树皮和杨圆蚧1龄因定若虫的介壳对长棒四节蚜小蜂和黄胸扑虱蚜小蜂成虫具有较大的吸引力。3-7μL介壳挥发油和5-9μL受害杨树皮挥发油对长棒四节蚜小蜂的寄主搜寻行为具有较强的引导作用。虫体的挥发油对小蜂的寄生定位也起到了一定的引诱作用。两种小蜂对健康树皮的挥发油几乎没有反应。图4表1参20。 展开更多
关键词 Populus pseudo-simonii ×P. nigra Quadraspidiotus gigas (Thiem & Gerneck) Pteroptrix longgiclava (Girault) Encarsia gigas (Tshumakova) Essential Oil PARASITOID Foraging behavior
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基于UF、GFC及RP-HPLC的长牡蛎(Crassostrea gigas)蛋白抗衰老小分子活性肽(Zel’ner)的纯化及结构解析 被引量:1
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作者 何定芬 谢超 +2 位作者 梁佳 梁瑞萍 李海波 《海洋与湖沼》 CAS CSCD 北大核心 2020年第1期141-147,共7页
牡蛎肉质鲜美,营养丰富,具有很高的食用价值。本研究以舟山长牡蛎(Crassostrea gigas)为研究对象,以DPPH自由基清除率为测试指标,通过UF、GFC及RP-HPLC等技术,对牡蛎蛋白酶解液进行分离纯化,获得牡蛎蛋白小分子肽样品,并对其氨基酸组成... 牡蛎肉质鲜美,营养丰富,具有很高的食用价值。本研究以舟山长牡蛎(Crassostrea gigas)为研究对象,以DPPH自由基清除率为测试指标,通过UF、GFC及RP-HPLC等技术,对牡蛎蛋白酶解液进行分离纯化,获得牡蛎蛋白小分子肽样品,并对其氨基酸组成进行营养性评价。研究结果表明:浓度为30%-100%的硫酸铵处理酶解液的分离效果明显优于低于浓度为30%硫酸铵的分离效果,其DPPH自由基清除率达到52.43%;纯化后得到两种牡蛎蛋白小分子肽分别标记为组分a(Mw<1kDa)和组分b(1kDa<Mw<3kDa);组分a中牡蛎蛋白小分子肽分子量分布主要为1995.2Da、1258.9Da、1023.3Da、398.1Da;对牡蛎蛋白小分子肽组分a进行检测,发现其中氨基酸种类及含量丰富,其中必需氨基酸(EAA)含量为18.863%,总氨基酸含量(TAA)为43.3748%,必需氨基酸占总氨基酸43.49%,必需氨基酸与非必需氨基酸(NEAA)的比例为76.95%,风味氨基酸(FAA)含量为18.9147%,占总氨基酸含量的43.61%。综上可见,牡蛎蛋白小分子肽具有极高的营养价值和市场经济效益,此研究为牡蛎蛋白抗衰老小分子活性肽(Zel'ner)的开发提供了新的思路和方向。 展开更多
关键词 长牡蛎(Crassostrea gigas) 小分子肽(Zel'ner) 分离纯化 氨基酸分析
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基础教育数字化转型的国际动态研究——以日本GIGA School构想为例
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作者 张文超 《教育教学论坛》 2024年第1期29-32,共4页
教育数字化转型已成为国际热议话题,国际上各国成效显著的基础教育数字化转型政策与行动对解决我国基础教育数字化转型中的问题有借鉴意义。近年来日本通过GIGA School构想的实践正逐步实现一人一台电子终端的基础教育数字化转型。因此... 教育数字化转型已成为国际热议话题,国际上各国成效显著的基础教育数字化转型政策与行动对解决我国基础教育数字化转型中的问题有借鉴意义。近年来日本通过GIGA School构想的实践正逐步实现一人一台电子终端的基础教育数字化转型。因此,尝试从日本的相关政策中解析其转型目的以及落实措施,并提出细化教育数字化转型目标、明确教育数字化转型标准、搭建转型建设体系、构建多方面政策联动系统的实践启示。 展开更多
关键词 基础教育 数字化转型 日本GIGA School构想
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乳山长牡蛎(Crassostrea gigas)的抗性基因表达和生存环境的季节差异 被引量:5
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作者 魏钰恒 潘英 +4 位作者 刘圣 谭林涛 张国范 李莉 黄宝玉 《海洋通报》 CAS CSCD 北大核心 2020年第5期601-608,共8页
长牡蛎(Crassostrea gigas)是我国重要的海水养殖贝类,近年其大规模死亡现象频繁发生。为了解长牡蛎的免疫基因在季节上的表达模式变化规律及死亡爆发高峰期的水环境情况,在其主养区山东乳山进行了相关研究。分别在2018年1、3、5—9月... 长牡蛎(Crassostrea gigas)是我国重要的海水养殖贝类,近年其大规模死亡现象频繁发生。为了解长牡蛎的免疫基因在季节上的表达模式变化规律及死亡爆发高峰期的水环境情况,在其主养区山东乳山进行了相关研究。分别在2018年1、3、5—9月对该地区牡蛎鳃样品做了免疫基因定量分析,在6、7、9月对养殖海域的环境因子(水温、盐度、pH、溶解氧)、各时间点水体里的浮游植物数量及各采样点牡蛎条件指数的变化进行了研究。结果显示,HSP70在7月份高表达,其余5个抗性相关基因在8月表达水平达到峰值。在测量的三个月中,7月水温最高,6月和9月水温略低于7月,这一结果和表达模式相关。研究表明近岸的藻类丰度更大。因此,温度是影响长牡蛎存活的各环境因子中的主要因素,温度显著影响到体内抗性基因的表达情况并且间接影响到夏季牡蛎的条件指数。 展开更多
关键词 长牡蛎(Crassostrea gigas) 季节变化 基因定量 养殖环境
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长牡蛎(Crassostrea gigas)中ChIP-seq方法的建立 被引量:1
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作者 展望 王文 施威扬 《海洋与湖沼》 CAS CSCD 北大核心 2015年第6期1557-1563,共7页
染色质免疫共沉淀技术(Ch IP)作为研究DNA结合蛋白与DNA相互作用最有力的工具,越来越受到重视。但目前该技术在牡蛎等贝类中的应用尚未见相关报道。为了研究长牡蛎早期胚胎发育过程中表观遗传信息的变化模式,本文在长牡蛎胚胎中探索并... 染色质免疫共沉淀技术(Ch IP)作为研究DNA结合蛋白与DNA相互作用最有力的工具,越来越受到重视。但目前该技术在牡蛎等贝类中的应用尚未见相关报道。为了研究长牡蛎早期胚胎发育过程中表观遗传信息的变化模式,本文在长牡蛎胚胎中探索并建立了一套完整的染色质免疫共沉淀方法,构建了高质量的高通量测序文库,首次获得了牡蛎全基因组水平的组蛋白H3K4me3修饰分布,从而为研究牡蛎的表观遗传调控奠定了基础。 展开更多
关键词 长牡蛎(Crassostrea gigas) CHIP-SEQ 高通量测序 组蛋白修饰
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Age, maturation, and population structure of the Humboldt squid Dosidicus gigas off the Peruvian Exclusive Economic Zones 被引量:22
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作者 刘必林 陈新军 +4 位作者 陈勇 田思泉 李建华 方舟 杨铭霞 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2013年第1期81-91,共11页
Age, maturation and population structure of the Humboldt squid Dosidicus gigas were studied based on random sampling of the Chinese jigging fishery off the Peruvian Exclusive Economic Zones (EEZ) during 2008-2010. E... Age, maturation and population structure of the Humboldt squid Dosidicus gigas were studied based on random sampling of the Chinese jigging fishery off the Peruvian Exclusive Economic Zones (EEZ) during 2008-2010. Estimated ages ranged from 144 to 633 days, confirming that the squid is a short-lived species with longevity no longer than 2 years. Occurrence of mature females and hatching in each month indicated that Humboldt squid spawned year-round. Back-calculated hatching dates for the samples were from January 22^nd, 2008 to April 22nd, 2010 with a peak between January and March. Two size-based and two hatching date-based populations could be defined from mantle length (ML) at maturity and back-calculated hatching dates, respectively. Females matured at a larger size than males, and there was a significant difference in ML at maturity between the two hatching groups (P〈0.05). The waters adjacent to 1 l^S off the Peruvian EEZ may be a potential spawning ground. This study shows the complexity of the population structure and large variability in key life history parameters in the Humboldt squid off the Peruvian EEZ, which should be considered in the assessment and management of this important resource. 展开更多
关键词 Dosidicus gigas offPeruvian EEZ age MATURATION population structure STATOLITH
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The impact of spatial scale on local Moran's I clustering of annual fishing effort for Dosidicus gigas offshore Peru 被引量:10
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作者 FENG Yongjiu CHEN Lijuan CHEN Xinjun 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2019年第1期330-343,共14页
The spatial scale(?shing grid) of ?sheries research af fects the observed spatial patterns of?sheries resources such as catch-per-unit-ef fort(CPUE) and ?shing ef fort. We examined the scale impact of high value(HH) c... The spatial scale(?shing grid) of ?sheries research af fects the observed spatial patterns of?sheries resources such as catch-per-unit-ef fort(CPUE) and ?shing ef fort. We examined the scale impact of high value(HH) clusters of the annual ?shing ef fort for Dosidicus gigas of fshore Peru from 2009 to 2012.For a multi-scale analysis, the original commercial ?shery data were tessellated to twelve spatial scales from 6′ to 72′ with an interval of 6′. Under these spatial scales, D. gigas clusters were identi?ed using the Anselin Local Moran's I. Statistics including the number of points, mean CPUE, standard deviation(SD),skewness, kurtosis, area and centroid were calculated for these HH clusters. We found that the z-score of global Moran's I and the number of points for HH clusters follow a power law scaling relationship from2009 to 2012. The mean ef fort and its SD also follow a power law scaling relationship from 2009 to 2012.The skewness follows a linear scaling relationship in 2010 and 2011 but ?uctuates with spatial scale in2009 and 2012; kurtosis follows a logarithmic scale relationship in 2009, 2011 and 2012 but a linear scale relationship in 2010. Cluster area follows a power law scaling relationship in 2010 and 2012, a linear scaling relationship in 2009, and a quadratic scaling relationship in 2011. Based on the peaks of Moran's I indices and the multi-scale analysis, we conclude that the optimum scales are 12′ in 2009 ? 2011 and 6′ in 2012, while the coarsest allowable scales are 48′ in 2009, 2010 and 2012, and 60′ in 2011. Our research provides the best spatial scales for conducting spatial analysis of this pelagic species, and provides a better understanding of scaling behavior for the ?shing ef fort of D. gigas in the of fshore Peruvian waters. 展开更多
关键词 Dosidicus gigas fishing ef fort high-high(HH) cluster scale impact local Moran's I
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Development of Genomic Microsatellite Multiplex PCR Using Dye-Labeled Universal Primer and Its Validation in Pedigree Analysis of Pacific Oyster(Crassostrea gigas) 被引量:5
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作者 LIU Ting LI Qi +1 位作者 SONG Junlin YU Hong 《Journal of Ocean University of China》 SCIE CAS CSCD 2017年第1期151-160,共10页
There is an increasing requirement for traceability of aquaculture products, both for consumer protection and for food safety. There are high error rates in the conventional traceability systems depending on physical ... There is an increasing requirement for traceability of aquaculture products, both for consumer protection and for food safety. There are high error rates in the conventional traceability systems depending on physical labels. Genetic traceability technique depending on DNA-based tracking system can overcome this problem. Genealogy information is essential for genetic traceability, and microsatellite DNA marker is a good choice for pedigree analysis. As increasing genotyping throughput of microsatellites, microsatellite multiplex PCR has become a fast and cost-effective technique. As a commercially important cultured aquatic species, Pacific oyster Crassostrea gigas has the highest global production. The objective of this study was to develop microsatellite multiplex PCR panels with dye-labeled universal primer for pedigree analysis in C. gigas, and these multiplex PCRs were validated using 12 full-sib families with known pedigrees. Here we developed six informative multiplex PCRs using 18 genomic microsatellites in C. gigas. Each multiplex panel contained a single universal primer M13(-21) used as a tail on each locus-specific forward primer and a single universal primer M13(-21) labeled with fluorophores. The polymorphisms of the markers were moderate, with an average of 10.3 alleles per locus and average polymorphic information content of 0.740. The observed heterozygosity per locus ranged from 0.492 to 0.822. Cervus simulations revealed that the six panels would still be of great value when massive families were analysed. Pedigree analysis of real offspring demonstrated that 100% of the offspring were unambiguously allocated to their parents when two multiplex PCRs were used. The six sets of multiplex PCRs can be an important tool for tracing cultured individuals, population genetic analysis, and selective breeding program in C. gigas. 展开更多
关键词 CRASSOSTREA gigas traceability microsatellites universal PRIMER multiplex PCR PEDIGREE analysis
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Biochemical Composition and Nutritional Value of Different Shell Color Strains of Pacific Oyster Crassostrea gigas 被引量:4
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作者 ZHU Yijing LI Qi +1 位作者 YU Hong KONG Lingfeng 《Journal of Ocean University of China》 SCIE CAS CSCD 2018年第4期897-904,共8页
The shell color of Pacific oyster(Crassostrea gigas) is a desirable trait; but the nutritional studies on C. gigas with different shell colors have not been conducted. Through successive selective breeding, five shell... The shell color of Pacific oyster(Crassostrea gigas) is a desirable trait; but the nutritional studies on C. gigas with different shell colors have not been conducted. Through successive selective breeding, five shell color strains of black(B), purple(P), orange(O), golden(G) and white(W) C. gigas have been developed. The aim of this study was to evaluate the chemical composition and nutritional value of five shell color strains and one commercial population with a common color. The biochemical composition including moisture, total protein, glycogen, ash, total fat, fatty acids(FA), amino acids and minerals was detected. The results indicated that the protein(50.76%–56.57%) was the major component. The content of glycogen showed a significant difference between orange shell and golden shell strains, as well as between commercial population and golden shell strain. In addition, all shell color strains contained a large amount of essential amino acids(12.20–14.15 g(100 g)^(-1)), of them leucine(2.81–3.29 g(100 g)^(-1)) and lysine(2.79–3.28 g(100 g)^(-1)) were predominant. The oysters were rich in polyunsaturated fatty acids(42.26%–45.24% of total fatty acid) with high levels of DHA(18.53%–21.16% of total fatty acid) and EPA(17.23%–18.68% of total fatty acid). Significant differences of mineral contents(Mg, Zn, Fe and Cu) were identified among the six populations. These results indicated that C. gigas with different shell colors presented rich nutritional value with high protein, glycogen, essential amino acids and polyunsaturated fatty acids. The biochemical composition obtained in this study is useful for selective breeding of C. gigas with different shell colors. 展开更多
关键词 CRASSOSTREA gigas BIOCHEMICAL composition NUTRITION value shell color selective breeding
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