Response surface methodology (RSM) was used to optimize the fermentation medium for enhancing pyruvic acid production by Torulopsis glabrata TP19. In the first step of optimization, with Plackett-Burman design, ammoni...Response surface methodology (RSM) was used to optimize the fermentation medium for enhancing pyruvic acid production by Torulopsis glabrata TP19. In the first step of optimization, with Plackett-Burman design, ammonium sulfate, glucose and nicotinic acid were found to be the important factors affecting pyruvic acid production significantly. In the second step, a 23 full factorial central composite design and RSM were applied to determine the optimal concentration of each significant variable. A second-order polynomial was determined by the multiple regression analysis of the experimental data. The optimum values for the critical components were obtained as follows: ammonium sulfate 0.7498 (10.75 g/L), glucose 0.9383 (109.38 g/L) and nicotinic acid 0.3633 (7.86 mg/L) with a predicted value of maximum pyruvic acid production of 42.2 g/L. Under the optimal conditions, the practical pyruvic acid production was 42.4 g/L. The determination coefficient (R2) was 0.9483, which ensures adequate credibility of the model. By scaling up fermentation from flask to jar fermentor, we obtained promising results.展开更多
The infection rate and cercarial production from B. glabrata, in comparison with B. alexandrina snails, post their exposure to S. mansoni miracidia of an E- gyptian strain after two cycles in albino-mice was stu-dies....The infection rate and cercarial production from B. glabrata, in comparison with B. alexandrina snails, post their exposure to S. mansoni miracidia of an E- gyptian strain after two cycles in albino-mice was stu-dies.The results indicated that infection rate of B. glabrata with the Egyptian strain of S. mansoni was less than that of B. alexandrina snails On the other hand infected B. glabrata exhibited a longer life span and a higher number of shedding cercariae, It was also, noticed that in the first cycle mice infected with S. mansoni cercariae shed from infected B. alexandrina snails, the mean number of worms recovered from infected mice was approximately twice that in mice infected with cercariae shed from infected B. glabrata snails The same observation was recorded from the mean number of ova/g liver tissue from infected mice. In the second cycle the same observation was recorded as first cycle suppression in the infection rate of B.glabrata than that B.alexandrina. Also, longer pre-patent period and life span Also, mice infection as the number of worms per infected mouse by cercariae shed from B.alexandrina snails was approximately 2.5 times that of mice infected by cercariae shed from B. glabrata being 29.3 and 12.5 worms/mouse.The results also indicated that the egg laying capacity of B. glabrata was higher than that B. alexandrina, It is concluded from this work that infectivity of S. man-soni cercariae shed from B. glabrata snails after two cycles of mice infection and used to infect the experi-mental final host was less than that of cercariae shed from infected B. alexandrina snails. This may declare a low compatibility of B. glabrata snails with the Egyptian strain of S. mansoni in comparison with B. alexandrina snails. However, this conclusion needs more passages of mice infection with cercariae to have precise data and conclusions.展开更多
Candidemia is defined as being a yeast infection confirmed by the presence of at least one positive Candida blood culture. It is a life threatening infection causing high mortality. The clinical signs are generally co...Candidemia is defined as being a yeast infection confirmed by the presence of at least one positive Candida blood culture. It is a life threatening infection causing high mortality. The clinical signs are generally compatible with the causative agent (whether there is a deep venous catheter or not). On the other hand and according to the 2012 Revised Chapel Hill Classification, granulomatosis with polyangiitis GPA is classified as a vasculitis associated with antineutrophil cytoplasmic antibodies ANCA. It is a systemic disease characterized by the anatomopathological aspect of granuloma. We report the case of a patient who presented an atypical and a very rare revealing mode of GPA which was a bronchopulmonary candidiasis complicated by candidemia. Despite its controversy, the combination in the acute phase of antifungal treatment based on intravenous voriconazole and glucocorticoid therapy has made it possible to control candidemia and calm vasculitis.展开更多
文摘Response surface methodology (RSM) was used to optimize the fermentation medium for enhancing pyruvic acid production by Torulopsis glabrata TP19. In the first step of optimization, with Plackett-Burman design, ammonium sulfate, glucose and nicotinic acid were found to be the important factors affecting pyruvic acid production significantly. In the second step, a 23 full factorial central composite design and RSM were applied to determine the optimal concentration of each significant variable. A second-order polynomial was determined by the multiple regression analysis of the experimental data. The optimum values for the critical components were obtained as follows: ammonium sulfate 0.7498 (10.75 g/L), glucose 0.9383 (109.38 g/L) and nicotinic acid 0.3633 (7.86 mg/L) with a predicted value of maximum pyruvic acid production of 42.2 g/L. Under the optimal conditions, the practical pyruvic acid production was 42.4 g/L. The determination coefficient (R2) was 0.9483, which ensures adequate credibility of the model. By scaling up fermentation from flask to jar fermentor, we obtained promising results.
文摘The infection rate and cercarial production from B. glabrata, in comparison with B. alexandrina snails, post their exposure to S. mansoni miracidia of an E- gyptian strain after two cycles in albino-mice was stu-dies.The results indicated that infection rate of B. glabrata with the Egyptian strain of S. mansoni was less than that of B. alexandrina snails On the other hand infected B. glabrata exhibited a longer life span and a higher number of shedding cercariae, It was also, noticed that in the first cycle mice infected with S. mansoni cercariae shed from infected B. alexandrina snails, the mean number of worms recovered from infected mice was approximately twice that in mice infected with cercariae shed from infected B. glabrata snails The same observation was recorded from the mean number of ova/g liver tissue from infected mice. In the second cycle the same observation was recorded as first cycle suppression in the infection rate of B.glabrata than that B.alexandrina. Also, longer pre-patent period and life span Also, mice infection as the number of worms per infected mouse by cercariae shed from B.alexandrina snails was approximately 2.5 times that of mice infected by cercariae shed from B. glabrata being 29.3 and 12.5 worms/mouse.The results also indicated that the egg laying capacity of B. glabrata was higher than that B. alexandrina, It is concluded from this work that infectivity of S. man-soni cercariae shed from B. glabrata snails after two cycles of mice infection and used to infect the experi-mental final host was less than that of cercariae shed from infected B. alexandrina snails. This may declare a low compatibility of B. glabrata snails with the Egyptian strain of S. mansoni in comparison with B. alexandrina snails. However, this conclusion needs more passages of mice infection with cercariae to have precise data and conclusions.
文摘Candidemia is defined as being a yeast infection confirmed by the presence of at least one positive Candida blood culture. It is a life threatening infection causing high mortality. The clinical signs are generally compatible with the causative agent (whether there is a deep venous catheter or not). On the other hand and according to the 2012 Revised Chapel Hill Classification, granulomatosis with polyangiitis GPA is classified as a vasculitis associated with antineutrophil cytoplasmic antibodies ANCA. It is a systemic disease characterized by the anatomopathological aspect of granuloma. We report the case of a patient who presented an atypical and a very rare revealing mode of GPA which was a bronchopulmonary candidiasis complicated by candidemia. Despite its controversy, the combination in the acute phase of antifungal treatment based on intravenous voriconazole and glucocorticoid therapy has made it possible to control candidemia and calm vasculitis.
