Glutathione （GSH） is the principal non-protein thiol responsible for maintaining intracellular redox status and protecting cells against oxidative/ nitrosative stresses. In the biological system, some GSH is bound t...Glutathione （GSH） is the principal non-protein thiol responsible for maintaining intracellular redox status and protecting cells against oxidative/ nitrosative stresses. In the biological system, some GSH is bound to proteins, and others exist freely, including the reduced glutathione and oxidized glutathione （GSSG）. Generally it is in a steady redox state for GSH/GSSG couple to participate in a variety of physiological functions. Earlier studies showed that chemically induced oxidative stress in vivo in rats resulted in increased GSSG and decreased GSH/GSSG ratio. The impairment of the glutathione homeostasis represents an indicator of tissue oxidative stresss status in human subjects.展开更多
内载谷胱甘肽(GSH)的大豆(Glycine max L.)下胚轴正向型质膜囊泡具有以GSH为电子供体的跨膜电子传递活性,能还原膜外电子受体FeCN和细胞色素(Cyt)C,其还原速率分别为(21.6±0.6)nmolFeCN·min^(-1)·mg^(-1)蛋白和(6.6±...内载谷胱甘肽(GSH)的大豆(Glycine max L.)下胚轴正向型质膜囊泡具有以GSH为电子供体的跨膜电子传递活性,能还原膜外电子受体FeCN和细胞色素(Cyt)C,其还原速率分别为(21.6±0.6)nmolFeCN·min^(-1)·mg^(-1)蛋白和(6.6±1.0)nmol Cyt C·min^(-1)·mg^(-1)蛋白。这种跨膜电子传递能引起膜上Cyt P-450吸收光谱标志带(Soret带)的变化,表明Cyt P-450参与了这一氧化还原过程。在跨质膜电子传递的同时伴随着H^+运输和膜电位的改变。展开更多
文摘Glutathione （GSH） is the principal non-protein thiol responsible for maintaining intracellular redox status and protecting cells against oxidative/ nitrosative stresses. In the biological system, some GSH is bound to proteins, and others exist freely, including the reduced glutathione and oxidized glutathione （GSSG）. Generally it is in a steady redox state for GSH/GSSG couple to participate in a variety of physiological functions. Earlier studies showed that chemically induced oxidative stress in vivo in rats resulted in increased GSSG and decreased GSH/GSSG ratio. The impairment of the glutathione homeostasis represents an indicator of tissue oxidative stresss status in human subjects.
