AIM To analyse the association between isoflavone intake and ulcerative colitis motility symptoms in individuals in remission. METHODS Cross-sectional study was conducted in a group of ulcerative colitis remission ind...AIM To analyse the association between isoflavone intake and ulcerative colitis motility symptoms in individuals in remission. METHODS Cross-sectional study was conducted in a group of ulcerative colitis remission individuals, in subgroups characterised by various intestinal motility and functioning characteristics(abdominal pain, flatulence, constipations, tenesmus). Total of 56 individuals with ulcerative colitis in remission(19 males and 37 females) were recruited for the study. Assessment of diet was based on self-reported data from each patient's dietary records taken over a period of three typical, random days(2 weekdays and 1 d of the weekend). The daily isoflavone intake(daidzein, genistein, glycitein and total isoflavones) and daily isoflavone intake per 1000 kcal of diet were assessed.RESULTS No correlations between isoflavone intake levels and number of bowel movements per day were observed both in the case of intake and intake per 1000 kcal of diet. In the group of individuals declaring lack of abdominal pain, the higher intakes of daidzein(P = 0.0075), daidzein per 1000 kcal of diet(P = 0.0358) and total isoflavone(P = 0.0358) were stated, than in the group of individuals declaring abdominal pain. In the group of individuals declaring lack of constipations, the lower intakes of glycitein(P = 0.0213) and glycitein per 1000 kcal of diet(P = 0.0213) were stated, than in the group of individuals declaring presence of constipations. No differences were observed in isoflavone intake between groups of ulcerative colitis individuals declaring lack of flatulence and declaring presence of flatulence, as well as between groups declaring lack of tenesmus and declaring presence of tenesmus.CONCLUSION The moderate dietary isoflavone intake may be beneficial for individuals with ulcerative colitis in remission, however, before including it into recommendations, further prospective studies are needed.展开更多
Soybean isoflavones compounds such as genistein, daidzein, and glycitein have numerous human health benefits including the reduction of risks of cardiovascular diseases, breast and prostate cancers, and menaupose symp...Soybean isoflavones compounds such as genistein, daidzein, and glycitein have numerous human health benefits including the reduction of risks of cardiovascular diseases, breast and prostate cancers, and menaupose symptoms in women. Understanding the genetic and environmental control of isoflavones accumulation is of great importance for developing new cultivars with high amounts of seed isoflavones. This study was conducted to analyze the effect of row spacing (25 cm vs. 50 cm) on seed isoflavones accumulation using a recombinant inbred line (RIL) population derived from the cross of PI 438489B and “Hamilton” (PIxH, n = 50). The two row spaces generated plant densities of 250,000 plants/ha and 90,000 plants/ha, respectively. Significant differences in soybean seed isoflavones (daidzein, genistein and glycitein) contents have been observed between plants grown in the two different plant densities. The mean daidzein content was 0.03458 μg·g-1 in plants grown in 50 cm row spaces (low plant density), which was significantly higher than its content (0.03019 μg·g-1) in plants grown in 25 cm row spaces (high plant density). Similarly, the mean glycitein content in plants grown in 50 cm row spaces (0.01905 μg·g-1) was significantly higher than its content in plants grown in 25 cm row spaces (0.00498 μg·g-1. Also, the mean genistein content in plants grown in 50 cm row spaces (0.01466 μg·g-1) was higher than its content in plants grown in 25 cm row spaces (0.00831 μg·g-1). These preliminary results are important in guiding farmers and breeders on choosing the best row spaces to grow soybean plants in order to optimize isoflavones contents. Further studies are needed to understand the correlation between seed isoflavones contents and other agronomic traits such as seed yield, protein, and oil contents.展开更多
目的建立同时测定淡豆豉中大豆苷、黄豆黄苷、染料木苷、大豆苷元、黄豆黄素、染料木素含量的方法。方法采用高效液相色谱法。采用Phenomenex Luna C 18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-0.1%甲酸水溶液(梯度洗脱),流速0.8 m...目的建立同时测定淡豆豉中大豆苷、黄豆黄苷、染料木苷、大豆苷元、黄豆黄素、染料木素含量的方法。方法采用高效液相色谱法。采用Phenomenex Luna C 18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-0.1%甲酸水溶液(梯度洗脱),流速0.8 mL·min^-1,检测波长254 nm,柱温30℃。结果大豆苷、黄豆黄苷、染料木苷、大豆苷元、黄豆黄素、染料木素分别在0.264~52.8(r=0.999 7)、0.278~55.6(r=0.999 9)、0.242~48.4(r=0.999 6)、0.256~51.2(r=0.999 9)、0.268~53.6(r=0.999 8)、0.256~51.2(r=0.999 7)μg·mL^-1范围内与峰面积呈良好的线性关系;并且大豆苷、黄豆黄苷、染料木苷、大豆苷元、黄豆黄素、染料木素平均加样回收率分别为98.88%、98.25%、99.34%、101.07%,95.23%和103.39%,RSD范围分别是2.34%、3.12%、2.38%、1.04%、1.86%和1.62%。结论建立的高效液相色谱方法操作简便、稳定、准确且可行,可用于淡豆豉的质量控制。展开更多
基金Supported by Ministry of Science and Higher Education Grant(WULS-SGGW:505-10-100400-L00332-99/2014)
文摘AIM To analyse the association between isoflavone intake and ulcerative colitis motility symptoms in individuals in remission. METHODS Cross-sectional study was conducted in a group of ulcerative colitis remission individuals, in subgroups characterised by various intestinal motility and functioning characteristics(abdominal pain, flatulence, constipations, tenesmus). Total of 56 individuals with ulcerative colitis in remission(19 males and 37 females) were recruited for the study. Assessment of diet was based on self-reported data from each patient's dietary records taken over a period of three typical, random days(2 weekdays and 1 d of the weekend). The daily isoflavone intake(daidzein, genistein, glycitein and total isoflavones) and daily isoflavone intake per 1000 kcal of diet were assessed.RESULTS No correlations between isoflavone intake levels and number of bowel movements per day were observed both in the case of intake and intake per 1000 kcal of diet. In the group of individuals declaring lack of abdominal pain, the higher intakes of daidzein(P = 0.0075), daidzein per 1000 kcal of diet(P = 0.0358) and total isoflavone(P = 0.0358) were stated, than in the group of individuals declaring abdominal pain. In the group of individuals declaring lack of constipations, the lower intakes of glycitein(P = 0.0213) and glycitein per 1000 kcal of diet(P = 0.0213) were stated, than in the group of individuals declaring presence of constipations. No differences were observed in isoflavone intake between groups of ulcerative colitis individuals declaring lack of flatulence and declaring presence of flatulence, as well as between groups declaring lack of tenesmus and declaring presence of tenesmus.CONCLUSION The moderate dietary isoflavone intake may be beneficial for individuals with ulcerative colitis in remission, however, before including it into recommendations, further prospective studies are needed.
文摘Soybean isoflavones compounds such as genistein, daidzein, and glycitein have numerous human health benefits including the reduction of risks of cardiovascular diseases, breast and prostate cancers, and menaupose symptoms in women. Understanding the genetic and environmental control of isoflavones accumulation is of great importance for developing new cultivars with high amounts of seed isoflavones. This study was conducted to analyze the effect of row spacing (25 cm vs. 50 cm) on seed isoflavones accumulation using a recombinant inbred line (RIL) population derived from the cross of PI 438489B and “Hamilton” (PIxH, n = 50). The two row spaces generated plant densities of 250,000 plants/ha and 90,000 plants/ha, respectively. Significant differences in soybean seed isoflavones (daidzein, genistein and glycitein) contents have been observed between plants grown in the two different plant densities. The mean daidzein content was 0.03458 μg·g-1 in plants grown in 50 cm row spaces (low plant density), which was significantly higher than its content (0.03019 μg·g-1) in plants grown in 25 cm row spaces (high plant density). Similarly, the mean glycitein content in plants grown in 50 cm row spaces (0.01905 μg·g-1) was significantly higher than its content in plants grown in 25 cm row spaces (0.00498 μg·g-1. Also, the mean genistein content in plants grown in 50 cm row spaces (0.01466 μg·g-1) was higher than its content in plants grown in 25 cm row spaces (0.00831 μg·g-1). These preliminary results are important in guiding farmers and breeders on choosing the best row spaces to grow soybean plants in order to optimize isoflavones contents. Further studies are needed to understand the correlation between seed isoflavones contents and other agronomic traits such as seed yield, protein, and oil contents.
