AIM To study the purifying method and characteristics of neW gosling viral enteritis virus (NGVEV), the etiological agent of new gosling viral enteritis (NGVE) which was first recognized in China, as well as the patho...AIM To study the purifying method and characteristics of neW gosling viral enteritis virus (NGVEV), the etiological agent of new gosling viral enteritis (NGVE) which was first recognized in China, as well as the pathomorphological davelapment in goslings infected artificially with NGVEV.``of treatment with chloroform and ammonium sulfate precipitation, dialysis to remove the sulfate radical and ammonium ion and separation by gel filtration Sichuan goslings were orally administered with NGVEV and 24hr later 2 birds were randomly selected and killed at 24hr intervals Until death occurred. Specimens(duodenum, ileum,liver, heart, kidney, spleen, lung, proventriculus, pancreas,esophagus, and the intestinal embolus) were taken until all birds in this group died and were sectioned and stained with hemotoxylin and ensin and studied by light microscope.``RESULTS NGVEV shared the typical characteristics of Adenovirus and which structural proteins consisted of 15polypsptides. Necrosis and sloughing of the epithelial cells covering the villus tips of the duodanum were first observed in goslings 2 days postinfectien artificially with NGVEV. With the progress of infection, this lesion rapidly occurred in the epithelium at the base of the villus and with infiltration of the inflammatory cells, the jejunum tended to be involved. With the intensification of rnucnsa necrosis and inflammatory exudation of the small intestine, fibrinenecrotic enteritis was further developed and embolus composed of either intestinal contents wrapped by pseudomembrane or of the mixture of fibrous exudate and necrotic intestinal rnucoes were observed in the middle-lower part of the small intestine. This structure occluded the intestinal tract and made the intestine dilated in appearance. The intestinal glandular cells underwent degeneration, necrosis and might be found sloughed into the lumen. Hemorrhage and hyperemia could be observed on the lung and kidney . Epithelial cells of the renal tubular underwent degeneration. In some cases, granular degeneration and fatty degeneration could be found in the liver and in some cases at a later stage of this disease the epithelial ceils of trachea and proventriculus might be found sioughed. In some cases at an early stage of this disease,cardiac hyperemia and hemorrhage could be observed.Esophaagus, pancreas and brain were found normal. Analysesand comparisons between the pathologic lesions of GVE and Gosling Plague (GP) were available in this paper as well.``characteristic could be as the data for NGVE diagnosis.展开更多
Twenty four 7-day-old crossbred goslings （Chuan white×Tai lake） were divided randomly into control and treatment groups. Enzyme preparation was added in the diet of treatment group at dosage of 1 g/kg. The bloo...Twenty four 7-day-old crossbred goslings （Chuan white×Tai lake） were divided randomly into control and treatment groups. Enzyme preparation was added in the diet of treatment group at dosage of 1 g/kg. The blood samples were collected from 60 day-old goslings via the wing vein. During the fifty-three days experiment, body weights were adopted measured each week, related hormone contents and biochemical parameters in plasma were determined. The results were as following:（1） Compared with control, body weight of treatment group were significantly higher （P<0.05） since 3-week-old goslings. The weight gains were significant higher （P<0.05） in （4～7） week-old goslings too, feed intake were increased and the "feed: body weight gain" was decreased; （2） Compared with control, growth hormone （GH） and insulin-like growth factor-I （IGF-I） levels were dramatically enhanced by 36.62% （P<0.01） and 66.55% （P<0.01） respectively, while the level of β-endorphin （β-END） decreased by 51.66% （P<0.01）; （3） Thyroid-stimulating hormone （TSH） and 3,3′,5-triiodothyronine （T3） contents in treatment group were 24.39% （P<0.05） and （16.81%） （P<0.05） higher than those of control, whereas thyroxine （T4） contents was lower by 18.33% （P<0.05）, ratios of T3/T4 were increased; （4） Compared with control, insulin contents were elevated by 36.75% （P<0.01）, while level of glucagon was reduced by 23.11% （P<0.01）,ratios of insulin/glucagon were increased; （5） The activity of glutamic-pyruvic transaminase （GTP） was 4.46 fold higher than those of control （P<0.01） and uric acid content was reduced by 57.50% （P<0.01）.These results indicate that enzyme preparation added in barley based diet can promote the gosling growth by improving the barely digestibility, carbohydrate metabolism and influencing the hormone levels.展开更多
大约八年前的今天,Java刚刚诞生后不久,《经济学家》在一篇题为“Will your next computer be a tin can and a wire?”的报道中画了一副有趣的漫画,一个人怀抱着一台电脑,电脑的一端连着一条线,线的另一端是一张网络(蜘蛛网),蜘蛛网上...大约八年前的今天,Java刚刚诞生后不久,《经济学家》在一篇题为“Will your next computer be a tin can and a wire?”的报道中画了一副有趣的漫画,一个人怀抱着一台电脑,电脑的一端连着一条线,线的另一端是一张网络(蜘蛛网),蜘蛛网上蹲坐着一只硕大无比的“戴着一副眼镜的蜘蛛”。今天,这只“蜘蛛”就笑眯眯的坐在我们面前,他就是Java之父James Gosling。展开更多
基金Supported by the Mational Mature Scientific Foundation of China,No.39970561Funds for the Leeders in Academia of Sichuan Province,No.9900009240018.
文摘AIM To study the purifying method and characteristics of neW gosling viral enteritis virus (NGVEV), the etiological agent of new gosling viral enteritis (NGVE) which was first recognized in China, as well as the pathomorphological davelapment in goslings infected artificially with NGVEV.``of treatment with chloroform and ammonium sulfate precipitation, dialysis to remove the sulfate radical and ammonium ion and separation by gel filtration Sichuan goslings were orally administered with NGVEV and 24hr later 2 birds were randomly selected and killed at 24hr intervals Until death occurred. Specimens(duodenum, ileum,liver, heart, kidney, spleen, lung, proventriculus, pancreas,esophagus, and the intestinal embolus) were taken until all birds in this group died and were sectioned and stained with hemotoxylin and ensin and studied by light microscope.``RESULTS NGVEV shared the typical characteristics of Adenovirus and which structural proteins consisted of 15polypsptides. Necrosis and sloughing of the epithelial cells covering the villus tips of the duodanum were first observed in goslings 2 days postinfectien artificially with NGVEV. With the progress of infection, this lesion rapidly occurred in the epithelium at the base of the villus and with infiltration of the inflammatory cells, the jejunum tended to be involved. With the intensification of rnucnsa necrosis and inflammatory exudation of the small intestine, fibrinenecrotic enteritis was further developed and embolus composed of either intestinal contents wrapped by pseudomembrane or of the mixture of fibrous exudate and necrotic intestinal rnucoes were observed in the middle-lower part of the small intestine. This structure occluded the intestinal tract and made the intestine dilated in appearance. The intestinal glandular cells underwent degeneration, necrosis and might be found sloughed into the lumen. Hemorrhage and hyperemia could be observed on the lung and kidney . Epithelial cells of the renal tubular underwent degeneration. In some cases, granular degeneration and fatty degeneration could be found in the liver and in some cases at a later stage of this disease the epithelial ceils of trachea and proventriculus might be found sioughed. In some cases at an early stage of this disease,cardiac hyperemia and hemorrhage could be observed.Esophaagus, pancreas and brain were found normal. Analysesand comparisons between the pathologic lesions of GVE and Gosling Plague (GP) were available in this paper as well.``characteristic could be as the data for NGVE diagnosis.
基金Item supported by international develop-ment research centre of Canada (No.92-1300)national sciencefoundation of China(No.39270500)
文摘Twenty four 7-day-old crossbred goslings （Chuan white×Tai lake） were divided randomly into control and treatment groups. Enzyme preparation was added in the diet of treatment group at dosage of 1 g/kg. The blood samples were collected from 60 day-old goslings via the wing vein. During the fifty-three days experiment, body weights were adopted measured each week, related hormone contents and biochemical parameters in plasma were determined. The results were as following:（1） Compared with control, body weight of treatment group were significantly higher （P<0.05） since 3-week-old goslings. The weight gains were significant higher （P<0.05） in （4～7） week-old goslings too, feed intake were increased and the "feed: body weight gain" was decreased; （2） Compared with control, growth hormone （GH） and insulin-like growth factor-I （IGF-I） levels were dramatically enhanced by 36.62% （P<0.01） and 66.55% （P<0.01） respectively, while the level of β-endorphin （β-END） decreased by 51.66% （P<0.01）; （3） Thyroid-stimulating hormone （TSH） and 3,3′,5-triiodothyronine （T3） contents in treatment group were 24.39% （P<0.05） and （16.81%） （P<0.05） higher than those of control, whereas thyroxine （T4） contents was lower by 18.33% （P<0.05）, ratios of T3/T4 were increased; （4） Compared with control, insulin contents were elevated by 36.75% （P<0.01）, while level of glucagon was reduced by 23.11% （P<0.01）,ratios of insulin/glucagon were increased; （5） The activity of glutamic-pyruvic transaminase （GTP） was 4.46 fold higher than those of control （P<0.01） and uric acid content was reduced by 57.50% （P<0.01）.These results indicate that enzyme preparation added in barley based diet can promote the gosling growth by improving the barely digestibility, carbohydrate metabolism and influencing the hormone levels.
文摘大约八年前的今天,Java刚刚诞生后不久,《经济学家》在一篇题为“Will your next computer be a tin can and a wire?”的报道中画了一副有趣的漫画,一个人怀抱着一台电脑,电脑的一端连着一条线,线的另一端是一张网络(蜘蛛网),蜘蛛网上蹲坐着一只硕大无比的“戴着一副眼镜的蜘蛛”。今天,这只“蜘蛛”就笑眯眯的坐在我们面前,他就是Java之父James Gosling。
