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猪丹毒丝菌SYBR GreenⅠ荧光定量PCR检测方法的建立
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作者 陈超 高春阳 +5 位作者 刘刚 姜志康 柳宇 张雪莲 袁生 韩先杰 《中国兽医科学》 CAS CSCD 2024年第3期337-343,共7页
为建立一种快速、 敏感的猪丹毒丝菌检测方法, 本试验将猪丹毒丝菌的 grol 基因与载体TA/ Blunt- Zero 相结合构建重组质粒,以该重组质粒 DNA 为模板,筛选最佳反应条件,建 立 SYBR Green Ⅰ荧光定量 PCR 检测方法,并对该方法进行灵敏度... 为建立一种快速、 敏感的猪丹毒丝菌检测方法, 本试验将猪丹毒丝菌的 grol 基因与载体TA/ Blunt- Zero 相结合构建重组质粒,以该重组质粒 DNA 为模板,筛选最佳反应条件,建 立 SYBR Green Ⅰ荧光定量 PCR 检测方法,并对该方法进行灵敏度、特异性和重复性测定。 结果表明,本研究建立的检测方法对在 2×10^(2)~2×10^(9)copies/ μL 浓度范围内的质粒标准品有良好的线性关系, 线性相关系数 R^(2)=0.999 5,标准曲线为 y=-3.231x+41.834,未见非特异性扩增。 该方法对质粒标准品最低检测浓度为 20 copies/ μL,敏感度比普通 PCR 提高了 1 000 倍。 用该方法检测猪支气管败血波氏杆菌(Bb)、猪链球菌 3 型(Ss3)、猪链球菌 2型(Ss2)、大肠杆菌(E.coli)、沙门菌(Salmonella)和多杀性巴氏杆菌(Pm)时,检测结果均为阴性,表明该方法具有良好的特异性。重复试验结果显示,组内和组间的变异系数均小于 2%,表明该方法具有良好的重复性。运用本试验建立的方法对 20 株疑似猪丹毒丝菌临床样品进行检测,阳性检出率为 60%,高于普通 PCR 检出率(50%)。 综上,本试验建立的检测方法灵敏度高、特异性强、重复性好,适合临床上快速诊断丹毒丝菌。 展开更多
关键词 猪丹毒丝菌 grol基因 SYBR greenⅠ荧光定量PCR 检测方法
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Does Green Food Certification promote agri-food export quality?Evidence from China
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作者 Ping Wei Hongman Liu +1 位作者 Chaokai Xu Shibin Wen 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第3期1061-1074,共14页
The construction of a food certification system plays a vital role in upgrading export quality, which previous studies have largely overlooked. We match China's industry-level data of Green Food Certification with... The construction of a food certification system plays a vital role in upgrading export quality, which previous studies have largely overlooked. We match China's industry-level data of Green Food Certification with its HS6-digit export data of agri-food products to quantify the impact of Green Food Certification on export quality. We identify the significant and positive effect of Green Food Certification on export quality. The 2SLS estimation based on instrumental variables and a range of robustness checks confirm the validity and robustness of the benchmark conclusions. Further analysis discloses that Green Food Certification improves export quality by raising agricultural production efficiency and brand premiums. Heterogeneity analysis shows that the effect of Green Food Certification varies across regions, notably improving the quality of agri-food products exported to developed regions and regions with high levels of import supervision. Furthermore, among various product types, Green Food Certification significantly improves the export quality of primary products and products vulnerable to non-tariff measures. The above findings could guide the future development of agri-food quality certification systems, potentially leading to a transformation and promotion of the agri-food trade. 展开更多
关键词 green Food Certification agri-food products green transformation export quality food labeling
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约束Green函数与非线性地基梁模态分析
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作者 赵翔 王琦 +1 位作者 朱伟东 李映辉 《动力学与控制学报》 2024年第2期77-84,共8页
在生物和医学领域,微机电系统(MEMS)中的微梁结构在植入人体的使用时,由于体内的细胞环境类似于水凝胶,在这种环境下工作,设备和仪器的精度和稳定性很大程度上受到细胞弹性的影响.为了分析此类地基梁的动力学问题,本文建立了非线性基础... 在生物和医学领域,微机电系统(MEMS)中的微梁结构在植入人体的使用时,由于体内的细胞环境类似于水凝胶,在这种环境下工作,设备和仪器的精度和稳定性很大程度上受到细胞弹性的影响.为了分析此类地基梁的动力学问题,本文建立了非线性基础上的梁振动模型,研究了任意位置弹簧和非线性弹簧基础上的梁模态.通过Laplace变换和线性叠加原理,得到了一种约束Green函数,利用数值计算验证方案的有效性,并研究了各种重要物理参数的影响,发现弹簧位置向跨中移动时,模态对称性被打破,弹簧刚度增加,模态阶数改变. 展开更多
关键词 约束green函数 LAPLACE变换 非线性弹簧基础 模态分析 叠加原理
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A green cross-linking method for the preparation of renewable threedimensional graphene sponges for efficient adsorption of Congo red dye
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作者 Zhuang Liu Bo Gao +3 位作者 Haoyuan Han Yuling Li Haiyang Fu Donghui Wei 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2024年第2期84-93,共10页
Graphene-based materials possess significant potential for the treatment of dye wastewater due to their exceptional adsorption properties toward stubborn pollutants.However,their utilization is hindered by high prepar... Graphene-based materials possess significant potential for the treatment of dye wastewater due to their exceptional adsorption properties toward stubborn pollutants.However,their utilization is hindered by high preparation costs,low yields,environmental pollution during synthesis,and challenges in regenerating the adsorbent.This study proposes a novel approach to address these limitations by developing nitrogen-doped three-dimensional(3D)polyvinyl alcohol(PVA)crosslinked graphene sponges(N-PGA)using a cross-linking method with ammonium carbonate.This method offers a relatively mild,environmentally friendly approach.Ammonium carbonate serves as both a reducing and modifying agent,facilitating the formation of the intrinsic structure of N-PGA and acting as a nitrogen source.Meanwhile,PVA is utilized as the cross-linking agent.The results demonstrate that N-PGA exhibits a favorable internal 3D hierarchical porous structure and possesses robust mechanical properties.The measured specific surface area(BET)of N-PGA was as high as406.538 m^(2)·g^(-1),which was favorable for its efficient adsorption of Congo red(CR)dye molecules.At an initial concentration of 50 mg·L^(-1),N-PGA achieved an impressive removal rate of 89.6%and an adsorption capacity of 112 mg·g^(-1)for CR dye.Furthermore,it retained 79%of its initial adsorption capacity after 10 cycles,demonstrating excellent regeneration performance.In summary,the synthesized N-PGA displays remarkable efficacy in the adsorption of CR dye in wastewater,opening up new possibilities for utilizing 3D porous graphene nanomaterials as efficient adsorbents in wastewater treatment. 展开更多
关键词 green chemistry Graphene sponge Adsorbents ADSORPTION Congo red Regeneration
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Eutrophication of Jiangsu Coastal Water and Its Role in the Formation of Green Tide
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作者 XIAO Mingyan SONG Weina +2 位作者 ZHANG Haibo SHI Xiaoyong SU Rongguo 《Journal of Ocean University of China》 CAS CSCD 2024年第1期109-118,共10页
Since 2007,the large-scale green tide caused by Ulva prolifera(U.prolifera)have occurred as a recurrent phenomenon in the southern Yellow Sea of China.Field surveys and satellite remote sensing showed that the small s... Since 2007,the large-scale green tide caused by Ulva prolifera(U.prolifera)have occurred as a recurrent phenomenon in the southern Yellow Sea of China.Field surveys and satellite remote sensing showed that the small scattered patches of green tide algae were first observed along the Porphyra agriculture area of the Subei Shoal in late April.In this study,we attempted to identify the role of eutrophication in the origin of the green tide in the Subei Shoal and its adjacent area.Subei Shoal and its adjacent area are characterized by rich nutrients,especially NO_(3)^(-)-N,NH_(4)^(+)-N,PO_(4)^(3-)-P,and other bioavailable components(such as urea-N and amino acids).In the spring of 2017,the average concentrations of NO_(3)^(-)-N were 19.01±11.01μmolL^(-1),accounting for 86.68%of the dis-solved inorganic nitrogen(DIN).In addition,the average concentration of NH4^(+)-N was 2.51±1.60μmolL^(-1).PO_(4)^(3-)-P had an average concentration of 0.14±0.13μmolL-1.The average concentrations of urea-N and total hydrolyzed amino acids(THAA)were 1.73±1.36μmolL^(-1)and 1.33±0.80μmolL^(-1),respectively.Rich nutritive substances play a key role in the rapid production of U.prolifera and make the Jiangsu coastal water an incubator for green tide. 展开更多
关键词 green tide NUTRIENTS ALGAE Ulva prolifera EUTROPHICATION Subei Shoal
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Artificial selection of the Green Revolution gene Semidwarf 1 is implicated in upland rice breeding
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作者 Shuliang Jiao Qinyan Li +6 位作者 Fan Zhang Yonghong Tao Yingzhen Yu Fan Yao Qingmao Li Fengyi Hu Liyu Huang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第3期769-780,共12页
Semidwarf breeding has boosted crop production and is a well-known outcome from the first Green Revolution. The Green Revolution gene Semidwarf 1(SD1), which modulates gibberellic acid(GA) biosynthesis, plays a princi... Semidwarf breeding has boosted crop production and is a well-known outcome from the first Green Revolution. The Green Revolution gene Semidwarf 1(SD1), which modulates gibberellic acid(GA) biosynthesis, plays a principal role in determining rice plant height. Mutations in SD1 reduce rice plant height and promote lodging resistance and fertilizer tolerance to increase grain production. The plant height mediated by SD1 also favors grain yield under certain conditions. However, it is not yet known whether the function of SD1 in upland rice promotes adaptation and grain production. In this study, the plant height and grain yield of irrigated and upland rice were comparatively analyzed under paddy and dryland conditions. In response to dryland environments, rice requires a reduction in plant height to cope with water deficits. Upland rice accessions had greater plant heights than their irrigated counterparts under both paddy and dryland conditions, and appropriately reducing plant height could improve adaptability to dryland environments and maintain high grain yield formation. Moreover, upland rice cultivars with thicker stem diameters had stronger lodging resistance, which addresses the lodging problem. Knockout of SD1 in the upland rice cultivar IRAT104 reduced the plant height and grain yield, demonstrating that the adjustment of plant height mediated by SD1 could increase grain production in dryland fields. In addition, an SD1 genetic diversity analysis verified that haplotype variation causes phenotypic variation in plant height. During the breeding history of rice, SD1 allelic mutations were selected from landraces to improve the grain yield of irrigated rice cultivars, and this selection was accompanied by a reduction in plant height. Thus, five known mutant alleles were analyzed to verify that functional SD1 is required for upland rice production. All these results suggest that SD1 might have undergone artificial positive selection in upland rice, which provides further insights concerning greater plant height in upland rice breeding. 展开更多
关键词 green Revolution Semidwarf 1 high-yield breeding upland rice plant height
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Genome-wide SNP markers provided insights into the reproductive strategy and genetic diversity of the green tide causative species Ulva prolifera in China
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作者 Song SUN Weiwei MA +2 位作者 Nan WANG Song FENG Yan SUN 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2024年第2期533-543,共11页
Ulva prolifera is the causative species of the annually occurring large-scale green tides in China since 2007.Its specific biological features on reproductivity strategies,as well as intra-species genetic diversity,ar... Ulva prolifera is the causative species of the annually occurring large-scale green tides in China since 2007.Its specific biological features on reproductivity strategies,as well as intra-species genetic diversity,are still largely unknown,especially at the genome level,despite their importance in understanding the formation and outbreak of massive green tides.In the present study,the restriction site-associated DNA genotyping approach(2b-RAD)was adopted to identify the genome-wide single-nucleotide polymorphisms(SNPs)of 54 individual thalli including samples collected from Subei Shoal in 2019 and Qingdao coast from 2019 to 2021.SNPs genotype results revealed that most of the thalli in 2019 and 2020 were haploid gametophytes,while only half of the thalli were gametophytes in 2021,indicating flexibility in the reproductive strategies for the formation of the green tides among different years and the dominance of asexual and vegetative reproductive mode for the floating period.Besides,population analysis was conducted,and it revealed a very low genetic diversity among samples from Subei Shoal and the Qingdao coast in the same year and a higher divergence among samples in different years.The results showed the efficiency of 2b-RAD in the exploration of SNPs in U.prolifera and provided the first genome-wide scale evidence for the origin of the large-scale green tides on the Qingdao coast.This study improved our understanding of the reproductive strategy and genetic diversity of the green tide causative species and will help further reveal the biological causes of the green tide in China. 展开更多
关键词 green tide Ulva prolifera 2b-RAD single-nucleotide polymorphism(SNP) reproductive strategy genetic diversity
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Enhancing Green Ammonia Electrosynthesis Through Tuning Sn Vacancies in Sn‑Based MXene/ MAX Hybrids
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作者 Xinyu Dai Zhen‑Yi Du +10 位作者 Ying Sun Ping Chen Xiaoguang Duan Junjun Zhang Hui Li Yang Fu Baohua Jia Lei Zhang Wenhui Fang Jieshan Qiu Tianyi Ma 《Nano-Micro Letters》 SCIE EI CAS CSCD 2024年第5期154-168,共15页
Renewable energy driven N_(2) electroreduction with air as nitrogen source holds great promise for realizing scalable green ammonia production.However,relevant out-lab research is still in its infancy.Herein,a novel S... Renewable energy driven N_(2) electroreduction with air as nitrogen source holds great promise for realizing scalable green ammonia production.However,relevant out-lab research is still in its infancy.Herein,a novel Sn-based MXene/MAX hybrid with abundant Sn vacancies,Sn@Ti_(2)CTX/Ti_(2)SnC–V,was synthesized by controlled etching Sn@Ti_(2)SnC MAX phase and demonstrated as an efficient electrocatalyst for electrocatalytic N2 reduction.Due to the synergistic effect of MXene/MAX heterostructure,the existence of Sn vacancies and the highly dispersed Sn active sites,the obtained Sn@Ti2CTX/Ti_(2)SnC–V exhibits an optimal NH_(3) yield of 28.4μg h^(−1) mg_(cat)^(−1) with an excellent FE of 15.57% at−0.4 V versus reversible hydrogen electrode in 0.1 M Na_(2)SO_(4),as well as an ultra-long durability.Noticeably,this catalyst represents a satisfactory NH3 yield rate of 10.53μg h^(−1) mg^(−1) in the home-made simulation device,where commercial electrochemical photovoltaic cell was employed as power source,air and ultrapure water as feed stock.The as-proposed strategy represents great potential toward ammonia production in terms of financial cost according to the systematic technical economic analysis.This work is of significance for large-scale green ammonia production. 展开更多
关键词 green ammonia synthesis N2 electroreduction Renewable energy SN MXene/MAX hybrid
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Don’t forget emergency surgery! Lessons to learn from elective indocyanine green-guided gastrointestinal interventions
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作者 Davina Perini Jacopo Martellucci 《World Journal of Gastrointestinal Surgery》 SCIE 2024年第2期270-275,共6页
Fluorescence-based imaging has found application in several fields of elective surgery,but there is still a lack of evidence in the literature about its use in the emergency setting.Clinical trials have consistently s... Fluorescence-based imaging has found application in several fields of elective surgery,but there is still a lack of evidence in the literature about its use in the emergency setting.Clinical trials have consistently shown that indocyanine green(ICG)-guided surgery can dramatically reduce the risk of postoperative complic-ations,length of in-hospital stay and total healthcare costs in the elective setting.It is well-known that emergency surgery has a higher complication rate than its elective counterpart,therefore an impelling need for research studies to explore,validate and develop this issue has been highlighted.The present editorial aims to provide a critical overview of currently available applications and pitfalls of ICG fluorescence in abdominal emergencies.Furthermore,we evidenced how the experience of ICG-fluorescence in elective surgery might be of great help in implementing its use in acute situations.In the first paragraph we analyzed the tips and tricks of ICG-guided cancer surgery that might be exploited in acute cases.We then deepened the two most described topics in ICG-guided emergency surgery:Acute cholecystitis and intestinal ischemia,focusing on both the advantages and limitations of green-fluorescence application in these two fields.In emergency situations,ICG fluorescence demonstrates a promising role in preventing undue intestinal resections or their entity,facilitating the detection of intestinal ischemic zones,identifying biliary tree anatomy,reducing post-operative complications,and mitigating high mortality rates.The need to improve its application still exists,therefore we strongly believe that the elective and routinary use of the dye is the best way to acquire the necessary skills for emer-gency procedures. 展开更多
关键词 Indocyanine green Fluorescence Navigation surgery ANGIOGRAPHY Emergency surgery Decision-making
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Synergistic effects of three traditional herbs green tea,mulberry leaf and corn silk on glucose uptake level of L6 myoblasts and the hypoglycemic mechanism
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作者 Jing-Na Zhou Ming-Yue Li +4 位作者 Ting-Ting Zhang Jing-Yang Lu Min Zhang Peng-Wei Zhuang Hai-Xia Chen 《Traditional Medicine Research》 2024年第7期1-12,共12页
Background:Green tea,mulberry leaf and corn silk are traditional herbs used in the prevention and treatment of diabetes in China for a long time,but their synergistic hypoglycemic effects and mechanisms remain unclear... Background:Green tea,mulberry leaf and corn silk are traditional herbs used in the prevention and treatment of diabetes in China for a long time,but their synergistic hypoglycemic effects and mechanisms remain unclear.Methods:The effective components of green tea,mulberry leaf and corn silk were extracted and enriched.Mixture design of experiments was used to study the influences of different combinations on the cell viability and glucose uptake level of L6 myoblasts,so as to determine the optimal synergistic hypoglycemic combination.The possible hypoglycemic mechanism of the optimal synergistic combination was explored by cytotoxicity assay,glucose uptake assay,and western blot.Results:Three polyphenol enrichment fractions of the herbs,30%ethanol elution fraction of green tea(GT),50%ethanol elution fraction of mulberry leaf(ML)and 60%ethanol elution fraction of corn silk(CS)were obtained.The antioxidant activities of GT-30%,ML-50%and CS-60%were superior to those of crude extracts,and showed strong potential inα-amylase andα-glucosidase inhibition activities.The optimal synergistic combination of crude extracts G7(crude extract of green tea:crude extract of mulberry leaf:crude extract of corn silk=1:5:3),polyphenol enrichment fractions R3(GT-30%:ML-50%:CS-60%=1:7:1)and monomers X2(epigallocatechin gallate:morusin:formononetin=3:1:2)were selected,respectively.G7,R3,and X2 showed promoting effects on the cell viability and glucose uptake of L6 myoblasts within the detected concentration range.In addition,G7,R3,and X2 could increase the expression levels of p-PI3K/PI3K and p-Akt/Akt in L6 myoblasts,and promote the translocation of Glut4,but G7 and R3 showed more significant effects.Conclusion:The synergistic hypoglycemic effects of green tea,mulberry leaf and corn silk had the characteristics of multiple-components and multiple-targets with p-PI3K/PI3K,p-Akt/Akt and the translocation of Glut4 signal pathways involved.The three traditional herbs might have the potential to be combined used for the prevention and treatment of diabetes based on the synergistic hypoglycemic effects. 展开更多
关键词 green tea mulberry leaf corn silk synergistic effect type 2 diabetes mellitus mechanism
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Indocyanine green:The guide to safer and more effective surgery
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作者 Pietro Fransvea Maria Michela Chiarello +2 位作者 Valeria Fico Maria Cariati Giuseppe Brisinda 《World Journal of Gastrointestinal Surgery》 SCIE 2024年第3期641-649,共9页
In this editorial we comment on the article by Kalayarasan and co-workers published in the recent issue of the World Journal of Gastrointestinal Surgery.The authors present an interesting review on the use of indocyan... In this editorial we comment on the article by Kalayarasan and co-workers published in the recent issue of the World Journal of Gastrointestinal Surgery.The authors present an interesting review on the use of indocyanine green fluo-rescence in different aspects of abdominal surgery.They also highlight future perspectives of the use of indocyanine green in mini-invasive surgery.Indo-cyanine green,used for fluorescence imaging,has been approved by the Food and Drug Administration and is safe for use in humans.It can be administered in-travenously or intra-arterially.Since its advent,there have been several advance-ments in the applications of indocyanine green,especially in the surgical field,such as intraoperative mapping and biopsy of sentinel lymph node,measurement of hepatic function prior to resection,in neurosurgical cases to detect vascular anomalies,in cardiovascular cases for patency and assessment of vascular ab-normalities,in predicting healing following amputations,in helping visualization of hepatobiliary anatomy and blood vessels,in reconstructive surgery,to assess flap viability and for the evaluation of tissue perfusion following major trauma and burns.For these reasons,the intraoperative use of indocyanine green has become common in a variety of surgical specialties and transplant surgery.Co-lorectal surgery has just lately begun to adopt this technique,particularly for perfusion visualization to prevent anastomotic leakage.The regular use of in-docyanine green coupled with fluorescence angiography has recently been pro-posed as a feasible tool to help improve patient outcomes.Using the best available data,it has been shown that routine use of indocyanine green in colorectal surgery reduces the rates of anastomotic leak.The use of indocyanine green is proven to be safe,feasible,and effective in both elective and emergency scenarios.However,additional robust evidence from larger-scale,high-quality studies is essential before incorporating indocyanine green guided surgery into standard practice. 展开更多
关键词 Indocyanine green Colorectal surgery Fluorescence-guided surgery Gastrointestinal surgery Hepato-biliary surgery Pancreatic surgery Surgical oncology
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Laparoscopic left hemihepatectomy guided by indocyanine green fluorescence: A cranial-dorsal approach
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作者 Xing-Ru Wang Xiao-Ju Li +6 位作者 Dan-Dan Wan Qian Zhang Tian-Xi Liu Zong-Wen Shen Hong-Xing Tong Yan Li Jian-Wei Li 《World Journal of Gastrointestinal Surgery》 SCIE 2024年第2期409-418,共10页
BACKGROUND Advancements in laparoscopic technology and a deeper understanding of intra-hepatic anatomy have led to the establishment of more precise laparoscopic hepatectomy(LH)techniques.The indocyanine green(ICG)flu... BACKGROUND Advancements in laparoscopic technology and a deeper understanding of intra-hepatic anatomy have led to the establishment of more precise laparoscopic hepatectomy(LH)techniques.The indocyanine green(ICG)fluorescence navi-gation technique has emerged as the most effective method for identifying hepatic regions,potentially overcoming the limitations of LH.While laparoscopic left hemihepatectomy(LLH)is a standardized procedure,there is a need for innova-tive strategies to enhance its outcomes.important anatomical markers,surgical skills,and ICG staining methods.METHODS Thirty-seven patients who underwent ICG fluorescence-guided LLH at Qujing Second People's Hospital between January 2019 and February 2022 were retrospectively analyzed.The cranial-dorsal approach was performed which involves dissecting the left hepatic vein cephalad,isolating the Arantius ligament,exposing the middle hepatic vein,and dissecting the parenchyma from the dorsal to the foot in order to complete the anatomical LLH.The surgical methods,as well as intra-and post-surgical data,were recorded and analyzed.Our hospital’s Medical Ethics Committee approved this study(Ethical review:2022-019-01).RESULTS Intraoperative blood loss during LLH was 335.68±99.869 mL and the rates of transfusion and conversion to laparotomy were 13.5%and 0%,respectively.The overall incidence of complications throughout the follow-up(median of 18 months;range 1-36 months)was 21.6%.No mortality or severe complications(level IV)were reported.CONCLUSION LLH has the potential to become a novel,standardized approach that can effectively,safely,and simply expose the middle hepatic vein and meet the requirements of precision surgery. 展开更多
关键词 Laparoscopic left hemihepatectomy Indocyanine green Cranial-dorsal approach Laparoscopic hepatectomy Arantius ligament Glissonean pedicle
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鸡圆环病毒SYBR GreenⅠ实时荧光定量PCR检测方法的建立
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作者 陈佳圣 赵天琪 +3 位作者 刘东华 孙祥茹 陈文德 李根 《中国家禽》 2024年第1期56-61,共6页
为快速准确检测鸡圆环病毒(CCV),试验根据CCV Rep基因设计特异性引物,以CCV阳性病料提取的DNA为模板进行PCR扩增,构建CCV重组质粒,建立检测CCV的SYBR GreenⅠ实时荧光定量PCR方法。结果显示:试验建立的方法对2.87×10^(8)~2.87×... 为快速准确检测鸡圆环病毒(CCV),试验根据CCV Rep基因设计特异性引物,以CCV阳性病料提取的DNA为模板进行PCR扩增,构建CCV重组质粒,建立检测CCV的SYBR GreenⅠ实时荧光定量PCR方法。结果显示:试验建立的方法对2.87×10^(8)~2.87×10^(1)copies/μL浓度范围的CCV重组质粒标准品呈现良好的线性关系,相关系数(R^(2))为0.9936,灵敏度比常规PCR高100倍;该方法对鸡传染性贫血病毒、新城疫病毒、传染性支气管炎病毒等常见鸡病毒性病原以及多杀性巴氏杆菌、禽致病性大肠杆菌、鸡白痢沙门氏菌无特异性扩增,批内和批间变异系数均不超过1%;该方法对60份疑似CCV感染临床样品检测结果显示,CCV阳性检出率(16.67%)高于常规PCR。上述结果表明,研究建立的CCV SYBR GreenⅠ实时荧光定量PCR方法具有良好的敏感性、特异性和重复性,检出限为2.87×10^(1)copies/μL,可以用于CCV的快速定量检测。 展开更多
关键词 鸡圆环病毒 SYBR greenⅠ实时荧光定量PCR 检测方法
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Technical Points of Green Prevention and Control Technology of Major Diseases and Pests in Lixian Rhubarb(Rheum palmatum L.)
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作者 Shuizhan PAN Lianhu SUN +2 位作者 Minyan LIU Zhenkun WANG Yicun WANG 《Plant Diseases and Pests》 2024年第1期22-25,共4页
Based on different types of diseases,pests and weeds in the whole growth period of rhubarb(sowing period-harvesting period),the corresponding green prevention and control technology is proposed,aiming to further reduc... Based on different types of diseases,pests and weeds in the whole growth period of rhubarb(sowing period-harvesting period),the corresponding green prevention and control technology is proposed,aiming to further reduce the application amount of pesticides and fertilizers in the production of medicinal sources of Lixian rhubarb during the"14 th Five-Year Plan"period.The results will provide a theoretical basis for increasing the promotion and application of agricultural prevention and control(including disease-resistant varieties,ecological regulation),physical prevention and control,biological prevention and control measures,thus ensuring effective protection of the ecological environment,green,healthy and sustainable development of traditional Chinese medicine agriculture in Longnan,and source quality of authentic medicinal materials. 展开更多
关键词 Lixian rhubarb Disease PEST WEED green prevention and control
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How R&D investment promotes green technology innovation in the context of digitalization?-An empirical analysis based on provincial panel data
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作者 LIU Jie LI Zhi-hui WEI Fang-xin 《Ecological Economy》 2024年第1期39-52,共14页
Green technology innovation is an important driving force and source to promote my country’s high-quality development,and it is the core path to achieve sustainable development.This paper uses my country’s provincia... Green technology innovation is an important driving force and source to promote my country’s high-quality development,and it is the core path to achieve sustainable development.This paper uses my country’s provincial panel data from 2016 to 2019 to study the impact mechanism of R&D investment on green technology innovation,and introduces the level of digitization,using the panel threshold model to discuss its role in the impact mechanism of R&D investment on green technology innovation.The study found that when the level of digitalization in a region is low,increasing R&D investment does not necessarily improve the ability of green technology innovation;when the level of digitalization is relatively high,R&D investment has a positive role in promoting green technology innovation.Therefore,it is necessary to improve policies to encourage enterprises to increase investment in research and development;at the same time,it is necessary to promote the coordinated development of digital foundation,digital investment,digital literacy,digital economy and digital application,and promote the deep integration of digitalization and green technology innovation. 展开更多
关键词 green technology innovation R&D investment digital level panel model
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线性偏微分方程中Green矩阵的傅里叶变换
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作者 刘梅 陆富强 《理论数学》 2024年第3期164-171,共8页
本文介绍了Gronwall不等式及傅里叶变换的性质与推论,应用傅里叶变换法分析三维Navier-Stokes- Poisson (NSP)方程与三维可压缩Navier-Stokes-Korteweg方程的格林矩阵,得到NSP方程与Navier-Stokes-Korteweg方程的傅里叶变换。
关键词 傅里叶变换 green矩阵 Navier-Stokes-Poisson方程
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Impact on Soil Organic C and Total Soil N from Cool- and Warm-Season Legumes Used in a Green Manure-Forage Cropping System
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作者 Clark B. Neely Francis M. Rouquette Jr. +3 位作者 Cristine L.S. Morgan Frank M. Hons William L. Rooney Gerald R. Smith 《Agricultural Sciences》 2024年第3期333-357,共25页
Annual forage legumes are important components of livestock production systems in East Texas and the southeastern US. Forage legumes contribute nitrogen (N) to cropping systems through biological N fixation, and their... Annual forage legumes are important components of livestock production systems in East Texas and the southeastern US. Forage legumes contribute nitrogen (N) to cropping systems through biological N fixation, and their seasonal biomass production can be managed to complement forage grasses. Our research objectives were to evaluate both warm- and cool-season annual forage legumes as green manure for biomass, N content, ability to enhance soil organic carbon (SOC) and soil N, and impact on post season forage grass crops. Nine warm-season forage legumes (WSL) were spring planted and incorporated as green manure in the fall. Forage rye (Secale cereale L.) was planted following the incorporation of WSL treatments. Eight cool-season forage legumes (CSL) were fall planted in previously fallow plots and incorporated as green manure in late spring. Sorghum-sudangrass (Sorghum bicolor x Sorghum bicolor var. sudanense) was planted over all treatments in early summer after forage rye harvest and incorporation of CSL treatments. Sorghum-sudangrass was harvested in June, August and September, and treatments were evaluated for dry matter and N concentration. Soil cores were taken from each plot, split into depths of 0 to 15, 15 to 30 and 30 to 60 cm, and soil C and N were measured using combustion analysis. Nylon mesh bags containing plant samples were buried at 15 cm and used to evaluate decomposition rate of above ground legume biomass, including change in C and N concentrations. Mungbean (Vigna radiata L. [Wilczek]) had the highest shoot biomass yield (6.24 t DM ha<sup>-1</sup>) and contributed the most total N (167 kg∙ha<sup>-1</sup>) and total C (3043 kg∙ha<sup>-1</sup>) of the WSL tested. Decomposition rate of WSL biomass was rapid in the first 10 weeks and very slow afterward. Winter pea (Pisum sativum L. spp. sativum), arrow leaf clover (Trifolium vesiculosum Savi.), and crimson clover (Trifolium incarnatum L.) were the most productive CSL in this trial. Austrian winter pea produced 8.41 t DM ha<sup>-1</sup> with a total N yield of 319 kg N ha<sup>-1</sup> and total C production of 3835 kg C ha<sup>-1</sup>. The WSL treatments had only small effects on rye forage yield and N concentration, possibly due to mineralization of N from a large SOC pool already in place. The CSL treatments also had only minimal effects on sorghum-sudangrass forage production. Winter pea, arrow leaf and crimson clover were productive cool season legumes and could be useful as green manure crops. Mungbean and cowpea (Vigna unguiculata [L.] Walp.) were highly productive warm season legumes but may include more production risk in green manure systems due to soil moisture competition. 展开更多
关键词 Annual Legumes Soil N Soil Organic C green Manure Deer Browse Forage Cropping Systems
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Measurement and Strategy Research on the Green Development Level for the New Energy Vehicle Industry in Chongqing City
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作者 HE Qian 《Journal of Literature and Art Studies》 2024年第3期247-264,共18页
This article takes 2016-2022 as the inspection period to construct an evaluation index system for the green development level of the new energy vehicle industry.The entropy method and comprehensive index are used to m... This article takes 2016-2022 as the inspection period to construct an evaluation index system for the green development level of the new energy vehicle industry.The entropy method and comprehensive index are used to measure the green development level of the new energy vehicle industry in Chongqing,and compared with neighboring provinces such as Yunnan,Guizhou,and Sichuan.Policy recommendations are proposed to promote the development of the new energy vehicle industry in Chongqing City. 展开更多
关键词 Chongqing City new energy vehicle industry green development level
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Green Hydrogen: Perspectives and Challenges in Using the Natural Gas Network in Ceará/Brazil
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作者 Francisco Alfredo de Castro Mona Lisa Moura de Oliveira +1 位作者 Lutero Carmo de Lima Daniel Silveira Serra 《Journal of Geoscience and Environment Protection》 2024年第3期70-94,共25页
Climate change, mainly caused by the use of non-renewable fuels, has raised global concerns and led to the search for less polluting energy sources, making hydrogen a promising energy alternative with the potential to... Climate change, mainly caused by the use of non-renewable fuels, has raised global concerns and led to the search for less polluting energy sources, making hydrogen a promising energy alternative with the potential to contribute to changes in the energy mix of various countries through the use of technologies that enable its production and use with low or zero carbon emissions. In this context, Brazil has aroused great interest from other countries in exploring its renewable resources for the production of hydrogen (green hydrogen). In this sense, the use of natural gas pipelines and the use of hydrogen in mixtures with natural gas have become the subject of studies due to their economically viable alternative for the immediate use of this energy vector. However, there are still technical and regulatory challenges regarding the integration of hydrogen into the existing natural gas pipeline network. In this context, the present study aims to address the effects of hydrogen interaction with the structure of natural gas pipeline steel and the regulatory barriers to the use of this network for the transportation of green hydrogen, particularly in the state of Ceará/Brazil. After extensive analysis of literature and regulatory documents, it was concluded that: 1) Ceará/Brazil has strong potential to meet the demand for green hydrogen through the use of solar and wind energy sources;2) there is feasibility for the adaptation or conversion of natural gas infrastructure for the transportation of green hydrogen;3) discussions regarding the regulatory competence of green hydrogen transportation and distribution through the natural gas network in Brazil are still incipient;4) the current regulation of the natural gas industry can serve as a subsidy for the regulation of green hydrogen and natural gas transportation. 展开更多
关键词 Decarbonization Natural Gas green Hydrogen Pipelines
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Existence of Monotone Positive Solution for a Fourth-Order Three-Point BVP with Sign-Changing Green’s Function
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作者 Junrui Yue Yun Zhang Qingyue Bai 《Open Journal of Applied Sciences》 2024年第1期63-69,共7页
This paper is concerned with the following fourth-order three-point boundary value problem , where , we discuss the existence of positive solutions to the above problem by applying to the fixed point theory in cones a... This paper is concerned with the following fourth-order three-point boundary value problem , where , we discuss the existence of positive solutions to the above problem by applying to the fixed point theory in cones and iterative technique. 展开更多
关键词 Fourth-Order Three-Point Boundary Value Problem Sign-Changing green’s Function Fixed Point Index Iterative Technique Monotone Positive Solution EXISTENCE
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