The stability of the ancient flood control levees is mainly influenced by water level fluctuations, groundwater concentration and rainfalls. This paper takes the Lanxi ancient levee as a research object to study the e...The stability of the ancient flood control levees is mainly influenced by water level fluctuations, groundwater concentration and rainfalls. This paper takes the Lanxi ancient levee as a research object to study the evolution laws of its seepage, displacement and stability before and after reinforcement with the upside-down hanging wells and grouting curtain through numerical simulation methods combined with experiments and observations. The study results indicate that the filled soil is less affected by water level fluctuations and groundwater concentration after reinforcement. A high groundwater level is detrimental to the levee's long-term stability, and the drainage issues need to be fully considered. The deformation of the reinforced levee is effectively controlled since the fill deformation is mainly borne by the upside-down hanging wells. The safety factors of the levee before reinforcement vary significantly with the water level. The minimum value of the safety factors is 0.886 during the water level decreasing period, indicating a very high risk of the instability. While it reached 1.478 after reinforcement, the stability of the ancient levee is improved by a large margin.展开更多
Objective:To compare the efficacy of anal adenectomy with virtual hanging wire and anal fistulotomy in the treatment of low anal fistula in infants and children.Methods:60 children with low anal fistula who were admit...Objective:To compare the efficacy of anal adenectomy with virtual hanging wire and anal fistulotomy in the treatment of low anal fistula in infants and children.Methods:60 children with low anal fistula who were admitted to our hospital from October 2021 to March 2022 and met the inclusion criteria were randomly divided into two groups of 30 cases each;the treatment group was treated with anal adenectomy and virtual hanging wire surgery,and the control group was treated with anal fistula resection.The clinical efficacy after treatment was compared.Results:The total effective rate of both groups was 96.67%and the difference between the two groups was not statistically significant(P>0.05).The postoperative pain score of the treatment group was lower than that of the control group(P<0.05).The length of hospitalization and healing time of the treatment group was lower than that of the control group(P<0.05).The anal function of the patients in both groups was normal,and there was no adverse reaction.Conclusion:Anal gland excision and virtual hanging surgery for the treatment of low anal fistula in infants and children have the advantages of mild pain,reduced length of hospitalization,short healing time,and better patient experience as compared to anal fistula excision.展开更多
Hanging roofs or high hang-ups.a common problem in sublevel caving mining,usually result in a large ore loss and undermine mining safety.This paper analyzed the formation of a hanging roof and showed that increased co...Hanging roofs or high hang-ups.a common problem in sublevel caving mining,usually result in a large ore loss and undermine mining safety.This paper analyzed the formation of a hanging roof and showed that increased confining pressure and reduced free surface were its main characteristics.In order to break down a hanging roof,a new method based on shock wave collision and stress superposition was developed.In this method,two blastholes containing multi-primer at different positions are simultaneously initiated at first.By doing this,a new free surface and a swell room can be created.After these holes are fired,a long delay time is given to the next blasthole so that the fragments from the first twohole blasting have enough time to fall down.This new method was applied to three hanging roofs in one production area,and all of them were successfully broken down.Field inspection indicated that almost no damage was caused in the nearby drifts/tunnels due to the new method.In addition,the far field vibrations were found to be smaller than the maximum vibrations induced by some other blasts.展开更多
BACKGROUND Hanging is one of the most commonly used methods for suicide in both sexes worldwide.In a number of countries,hanging mortality has increased over the last decades.Nevertheless,there is a scarcity of studie...BACKGROUND Hanging is one of the most commonly used methods for suicide in both sexes worldwide.In a number of countries,hanging mortality has increased over the last decades.Nevertheless,there is a scarcity of studies that have explored the patterns and trends for mortality of suicide by hanging on global,regional and national levels,as most evaluations are limited to certain populations.AIM To assess the trends of suicide mortality by hanging,strangulation,and suffocation in Serbia,from 1991 to 2020.METHODS This nationwide study,with epidemiological descriptive study design,was carried out based on official data.The age-standardized rates(ASRs,expressed per 100000 persons)were calculated by direct standardization,using the World Standard Population.Mortality trends from suicide by hanging were assessed using the joinpoint regression analysis:The average annual percent change(AAPC)with the corresponding 95%confidence interval(95%CI)was calculated.Age-period-cohort analysis was performed to address the possible underlying reasons for the observed suicide trends.RESULTS Over the 30-year period studied,there were 24340 deaths by hanging(17750 males and 6590 females)in Serbia.In 2020,the ASR of deaths by hanging was 4.5 per 100000 persons in both sexes together(7.6 in males vs 1.7 in females).The trends of suicide mortality by hanging decreased significantly between 1991 and 2020 in both males(AAPC=-1.7%per year;95%CI:-2.0 to-1.4)and females(AAPC=-3.5%per year;95%CI:-3.9 to-3.1).Mortality rates of suicide by hanging had a continuously decreasing tendency in both sexes together in all age groups:The only exception was among males in 40-49 age group,with an increasing trend of suicide by hanging from 1991 to 2011(by+0.3%per year).CONCLUSION The trends in suicide mortality by hanging have been decreasing in Serbia in the last three decades in both sexes,but this was more pronounced in women than in men.Despite the decreasing trends observed in mortality of suicide by hanging,further research is needed for better clarification of trends and help in suicide prevention in the future.展开更多
Hanging-drop method has been widely used to fabricate three-dimensional (3D) in vitro tissue models due to its advantages such as being easy to perform, inexpensive, and permitting precise control of cell spheroid for...Hanging-drop method has been widely used to fabricate three-dimensional (3D) in vitro tissue models due to its advantages such as being easy to perform, inexpensive, and permitting precise control of cell spheroid formation. The geometry of hanging drop may play a critical role on the formation of cell spheroids, which, however, has not been explored. In this study, we developed a modified hanging-drop platform that enables the production of cell spheroids in a high-throughput manner by controlling hanging drop geometry with defined spreading ring. The surface tension force is proportional to the spreading ring and gravitational force is determined by the droplet volume, and the geometry can be determined by the balance between surface tension and gravity.β-TC-6 cell spheroids with optimized diameters were fabricated as 3D in vitro islet models. The models show morphology similar to primary islets and have functionality that more closely resembles primary islets than two-dimensional cell culture. The developed platform holds great potential for engineering well-controlled in vitro tissue models for various applications such as physiological and pathological studies, drug screening, as well as transplantation for treatment purpose.展开更多
Root-mean-square distance Drms with characteristic of weighted-average is introduced in this article firstly. Drms can be used to capture the general proximity of a site to a dipping fault plane comparing with the rup...Root-mean-square distance Drms with characteristic of weighted-average is introduced in this article firstly. Drms can be used to capture the general proximity of a site to a dipping fault plane comparing with the rupture distance Drup and the seismogenic distance Dseis. Then, using Drup, Dseis and Drms, the hanging wall/footwall effects on the peak ground acceleration (PGA) during the 1999 Chi-Chi earthquake are evaluated by regression analysis. The logarithm residual shows that the PGA on hanging wall is much greater than that on footwall at the same Drup or Dseis when the Drup or Dseis is used as site-to-source distance measure. In contrast, there is no significant difference between the PGA on hanging wall and that on footwall at the same Drms when Drms is used. This result confirms that the hanging wall/footwall effect is mainly a geometric effect caused by the asymmetry of dipping fault. Therefore, the hanging wall/footwall effect on the near-fault ground motions can be ignored in the future attenuation analysis if the root-mean-square distance Drms is used as the site-to-source distance measure.展开更多
Cibaliung Underground Gold Mine applies cut and fill mining method to optimize ore production and maintain underground stability. Existing 5 m × 5 m height and width of stope geometry has a potential new design t...Cibaliung Underground Gold Mine applies cut and fill mining method to optimize ore production and maintain underground stability. Existing 5 m × 5 m height and width of stope geometry has a potential new design to increase production of gold due to variety of thick ore, however serious shotcrete failures often occur in hanging wall decline. This paper aims to find out the relationship between stope design and stability of hanging wall decline. The analysis conducted in this study is based on underground characteristics and geological condition of Cibaliung area. The impact of stope design on decline stability was analyzed by using numerical methods. The impact factors such as different rock mass, size of stope, and distance between stope and hanging wall decline were used in the analysis of underground stability especially stability on hanging wall decline.展开更多
Based on the Han Dynasty stone reliefs unearthed in the ancient Xuzhou area,a kind of pavilion above the water—hanging waterside pavilion(Xuan Shui Xie,built above the water with stilts and brackets as the base and s...Based on the Han Dynasty stone reliefs unearthed in the ancient Xuzhou area,a kind of pavilion above the water—hanging waterside pavilion(Xuan Shui Xie,built above the water with stilts and brackets as the base and seeming hanging on the water)was systematically collected and classifi ed into 2 major categories:single-bracket column hanging waterside pavilion and multiple-bracket column hanging waterside pavilion.The research showed that hanging waterside pavilion got ride of traditional pavilion’s reliance on rammed earth foundation.In addition to working as a connection part,brackets,or bucket arches(Dougong)had also tremendous load-bearing capacity,straight and curved brackets co-formed a giant multiple bracket system,staircases and one-side hanging structure were used to support the upper construction,so that a new towering but light wooden structure hanging above the water was created,which was an innovation of bracket technique of the Han Dynasty,and also a creative design in the development of wooden architecture.展开更多
As a person in the petroleum industry,I worked in Bangladesh for many years.When I lived in the Legation Quarter in Dacca,the capital of Bangladesh,I liked wearing the most popular flip-flops.When I lived in the Custo...As a person in the petroleum industry,I worked in Bangladesh for many years.When I lived in the Legation Quarter in Dacca,the capital of Bangladesh,I liked wearing the most popular flip-flops.When I lived in the Customs Logistics Park in Chittagong,I liked being bound with an exotic headscarf.When I was stationed at a well site in Sylhet,I liked dressing in my dazzling"Petroleum Red".During my period working in Bangladesh,I was impressed by the crowded roads of the country.Especially,展开更多
This paper retrospectively analyzes the complicated rectovaginal fistula in 2 cases after the successful treatment. Through literatures collected, with the difficulties and contradictions of the treatment of complicat...This paper retrospectively analyzes the complicated rectovaginal fistula in 2 cases after the successful treatment. Through literatures collected, with the difficulties and contradictions of the treatment of complicated rectovaginal fistula as the starting point, the currently used hanging line method, the advantage and deficiency of surgical treatment in recent years, and the change of treatment method were reviewed. We wish to explore which current treatments could be a better choice.展开更多
A national flag is the representative symbol of a country.It is displayed on many occasions in society.A code comprised of three letters is delegated to each countryand is often used in
This series of hanging charts is easy to read and contains the common symbols used in daily life.The complete series of hanging charts includes traffic,safety and environmental protection symbols。
Objective: Conservative restoration principles that accurately reduce plaque accumulation are the goals of restorative dentistry. Correct and perfect restoration has an important effect on health of periodontious tiss...Objective: Conservative restoration principles that accurately reduce plaque accumulation are the goals of restorative dentistry. Correct and perfect restoration has an important effect on health of periodontious tissues. This study was done to compare the methods of oral hygiene, over hanged removal and restoration changing on periodontal health and alveolar bone height of restored mandible molars with proximal over hanged amalgam. Materials and Methods: All patients with over hanged amalgam restoration were randomly divided into three groups. In order to survey the effect of over hanged removal on gingival and bleeding indices and alveolar bone height adjacent to mandible molar teeth, these indices were determined according to recurrent radio-graphical early signs of alveolar bone loss during three months. Sixty patients, 20 in each group, were studied. In the group I, overhang was removed with ultrasonic scale or bur and oral hygiene methods as usual were continued. In the group II, over-hang was left, but plaque controls as the prophylaxis and flossing were done. In the group III, restoration was changed and oral hygiene was done as usual. Plaque index and bleeding index were measured at the beginning of study and three months later. Findings: Statistical analysis of studied indices at the beginning and three months after treatment using paired test has shown significant differences in gingival inflammation, in all groups. Statistical analysis of indices using Tukey HSD test has also shown significant differences in decreasing gingival inflammation measure when the over hanged restoration was removed. While there is no difference between the other two groups. Conclusion: The results showed that plaque control causes subsidence of gingival inflammation and increasing of alveolar bone support, and it is more effective when the over hanged restoration is changed.展开更多
Microfluidics is becoming a technology of growing interest for building microphysiological systems with integrated read-out functionalities.Here we present the integration of enzyme-based multi-analyte biosensors into...Microfluidics is becoming a technology of growing interest for building microphysiological systems with integrated read-out functionalities.Here we present the integration of enzyme-based multi-analyte biosensors into a multi-tissue culture platform for‘body-on-a-chip’applications.The microfluidic platform is based on the technology of hanging-drop networks,which is designed for the formation,cultivation,and analysis of fluidically interconnected organotypic spherical three-dimensional(3D)microtissues of multiple cell types.The sensor modules were designed as small glass plug-ins featuring four platinum working electrodes,a platinum counter electrode,and an Ag/AgCl reference electrode.They were placed directly into the ceiling substrate from which the hanging drops that host the spheroid cultures are suspended.The electrodes were functionalized with oxidase enzymes to enable continuous monitoring of lactate and glucose through amperometry.The biosensors featured high sensitivities of 322±41 nA mM^(−1) mm^(−2) for glucose and 443±37 nA mM^(−1) mm^(−2) for lactate;the corresponding limits of detection were below 10μM.The proposed technology enabled tissue-size-dependent,real-time detection of lactate secretion from single human colon cancer microtissues cultured in the hanging drops.Furthermore,glucose consumption and lactate secretion were monitored in parallel,and the impact of different culture conditions on the metabolism of cancer microtissues was recorded in real-time.展开更多
As 3D in vitro tissue models become more pervasive,their built-in nutrient,metabolite,compound,and waste gradients increase biological relevance at the cost of analysis simplicity.Investigating these gradients and the...As 3D in vitro tissue models become more pervasive,their built-in nutrient,metabolite,compound,and waste gradients increase biological relevance at the cost of analysis simplicity.Investigating these gradients and the resulting metabolic heterogeneity requires invasive and time-consuming methods.An alternative is using electrochemical biosensors and measuring concentrations around the tissue model to obtain size-dependent metabolism data.With our hanging-dropintegrated enzymatic glucose biosensors,we conducted current measurements within hanging-drop compartments hosting spheroids formed from the human colorectal carcinoma cell line HCT116.We developed a physics-based mathematical model of analyte consumption and transport,considering(1)diffusion and enzymatic conversion of glucose to form hydrogen peroxide(H_(2)O_(2))by the glucose-oxidase-based hydrogel functionalization of our biosensors at the microscale;(2)H_(2)O_(2)oxidation at the electrode surface,leading to amperometric H_(2)O_(2)readout;(3)glucose diffusion and glucose consumption by cancer cells in a spherical tissue model at the microscale;(4)glucose and H_(2)O_(2)transport in our hangingdrop compartments at the macroscale;and(5)solvent evaporation,leading to glucose and H_(2)O_(2)upconcentration.Our model relates the measured currents to the glucose concentrations generating the currents.The low limit of detection of our biosensors(0.4±0.1μM),combined with our current-fitting method,enabled us to reveal glucose dynamics within our system.By measuring glucose dynamics in hanging-drop compartments populated by cancer spheroids of various sizes,we could infer glucose distributions within the spheroid,which will help translate in vitro 3D tissue model results to in vivo.展开更多
Adaptive higher-order finite element methods(hp-FEM)are well known for their potential of exceptionally fast(exponential)convergence.However,most hp-FEM codes remain in an academic setting due to an extreme algorithmi...Adaptive higher-order finite element methods(hp-FEM)are well known for their potential of exceptionally fast(exponential)convergence.However,most hp-FEM codes remain in an academic setting due to an extreme algorithmic complexity of hp-adaptivity algorithms.This paper aims at simplifying hpadaptivity for H(curl)-conforming approximations by presenting a novel technique of arbitrary-level hanging nodes.The technique is described and it is demonstrated numerically that it makes adaptive hp-FEM more efficient compared to hp-FEM on regular meshes and meshes with one-level hanging nodes.展开更多
Hanging wall syncline and central dome are special extension structures, developing over the hanging wall in an extensional ramp-flat fault. Under the condition that the flat is sub-horizontal, the hanging wall syncli...Hanging wall syncline and central dome are special extension structures, developing over the hanging wall in an extensional ramp-flat fault. Under the condition that the flat is sub-horizontal, the hanging wall syncline is separated from the half graben by the central dome. And on the dome forms an erosional surface. Both sediments in the half graben and erosional surface on the top of the central dome extended over the dome and entered into the hanging wall syncline with extension going on. Meanwhile, those having entered were overlapped by new sedimentary layers in the hanging wall syncline, so that there is a together-threaded, diachronic unconformity to form in the same epoch stratum. The layers in the hanging wall syncline also have an attribute of migrating laterally and getting tilted with extension. There is no sedimentation on the central dome. But sediments, which came from the half graben, got thicker over the dome in extension.展开更多
Mechanical damage induced by vibration during harvesting and post-harvest handling could decrease the quality,quantity,and shelf life of the fresh grape cluster.Usually,fresh grape clusters are harvested by gripping a...Mechanical damage induced by vibration during harvesting and post-harvest handling could decrease the quality,quantity,and shelf life of the fresh grape cluster.Usually,fresh grape clusters are harvested by gripping and cutting from the main rachis in the present robotic harvesting system,then transported towards the basket during post-harvest handling.However,serious cluster vibration and corresponding berry falling may occur during the robotic transportation of hanging grape clusters.Therefore,this study was designed to perform experimental and theoretical hanging force analysis to explore the vibration mechanism of hanging grape clusters during robotic transportation.A lead screw lathe with an attached linear actuator was used to investigate the effects of four different speeds(0.4,0.6,0.8,1.0 m/s)with four acceleration levels(6,8,10,12 m/s2)on the vibration of the hanging grape cluster.By the experiments,the peak hanging force of the grape cluster at the start,constant speed,and stop phase of the actuator was recorded using a single axis force sensor,and the cluster’s swing angle was measured with a digital camera.The experimental results showed a linear relationship between the swing angle and hanging force of the cluster at the start and stop phase of the actuator.The multi-stage cluster’s vibration during robotic transportation was observed,and the behavior of cycled damping after a sudden stop of the actuator was found.The simulated results of hanging force of grape cluster in damping phase were agreed with experimental results with R2 more than 0.90 at an optimum acceleration of 10 m/s2.To conclude,this research provides theoretical basics for understanding the complex vibration mechanism of the hanging cluster fruits during speedy robotic transportation operations with low-loss of berry drop both on industrial and farm levels.展开更多
Violence against the neck can result in a range of macromorphological and micromorphological findings.However,the forensic relevance of the carotid sinus in cases of violence against the neck remains controversial.In ...Violence against the neck can result in a range of macromorphological and micromorphological findings.However,the forensic relevance of the carotid sinus in cases of violence against the neck remains controversial.In this follow-up study of 22 cases of suicidal and accidental strangulations,carotid bifurcations were examined histologically for morphological changes implying direct trauma,including haemorrhage and immunohistochemical expression of heat-shock proteins 27,60,and 70 and aquaporin-3.These cases were compared with a control group(82 cases)without neck compression or head trauma and with variable causes of death.No relevant histopathological findings implying direct trauma of the carotid bifurcation were found.No cases showed positive aquaporin-3 staining and only five cases showed positive heat-shock protein-27 staining,all of which were hangings.Without massive trauma of the carotid bifurcation,histological alterations cannot be expected.Without signs of rapid death,findings of acute circulatory failure,macromorphological and micromorphological findings of neck compression,and reliable markers indicating relevant impact on the carotid bifurcation the diagnosis of a lethal reflex cannot be verified.展开更多
基金the scientific research foundation of Zhejiang Provincial Natural Science Foundation of China (LTGG24E090002)Zhejiang University of Water Resources and Electric Power (xky2022013)+1 种基金Major Science and Technology Plan Project of Zhejiang Provincial Department of Water Resources (RA1904)the water conservancy management department, Zhejiang Design Institute of Water Conservancy and Hydro Electric Power Co., Ltd. and the construction company for their support。
文摘The stability of the ancient flood control levees is mainly influenced by water level fluctuations, groundwater concentration and rainfalls. This paper takes the Lanxi ancient levee as a research object to study the evolution laws of its seepage, displacement and stability before and after reinforcement with the upside-down hanging wells and grouting curtain through numerical simulation methods combined with experiments and observations. The study results indicate that the filled soil is less affected by water level fluctuations and groundwater concentration after reinforcement. A high groundwater level is detrimental to the levee's long-term stability, and the drainage issues need to be fully considered. The deformation of the reinforced levee is effectively controlled since the fill deformation is mainly borne by the upside-down hanging wells. The safety factors of the levee before reinforcement vary significantly with the water level. The minimum value of the safety factors is 0.886 during the water level decreasing period, indicating a very high risk of the instability. While it reached 1.478 after reinforcement, the stability of the ancient levee is improved by a large margin.
基金Xi’an Municipal Bureau of Science and Technology,No.21YXYJ0060。
文摘Objective:To compare the efficacy of anal adenectomy with virtual hanging wire and anal fistulotomy in the treatment of low anal fistula in infants and children.Methods:60 children with low anal fistula who were admitted to our hospital from October 2021 to March 2022 and met the inclusion criteria were randomly divided into two groups of 30 cases each;the treatment group was treated with anal adenectomy and virtual hanging wire surgery,and the control group was treated with anal fistula resection.The clinical efficacy after treatment was compared.Results:The total effective rate of both groups was 96.67%and the difference between the two groups was not statistically significant(P>0.05).The postoperative pain score of the treatment group was lower than that of the control group(P<0.05).The length of hospitalization and healing time of the treatment group was lower than that of the control group(P<0.05).The anal function of the patients in both groups was normal,and there was no adverse reaction.Conclusion:Anal gland excision and virtual hanging surgery for the treatment of low anal fistula in infants and children have the advantages of mild pain,reduced length of hospitalization,short healing time,and better patient experience as compared to anal fistula excision.
文摘Hanging roofs or high hang-ups.a common problem in sublevel caving mining,usually result in a large ore loss and undermine mining safety.This paper analyzed the formation of a hanging roof and showed that increased confining pressure and reduced free surface were its main characteristics.In order to break down a hanging roof,a new method based on shock wave collision and stress superposition was developed.In this method,two blastholes containing multi-primer at different positions are simultaneously initiated at first.By doing this,a new free surface and a swell room can be created.After these holes are fired,a long delay time is given to the next blasthole so that the fragments from the first twohole blasting have enough time to fall down.This new method was applied to three hanging roofs in one production area,and all of them were successfully broken down.Field inspection indicated that almost no damage was caused in the nearby drifts/tunnels due to the new method.In addition,the far field vibrations were found to be smaller than the maximum vibrations induced by some other blasts.
基金Supported by Ministry of Education,Science and Technological Development,Republic of Serbia, 2011–2020, No. 175042
文摘BACKGROUND Hanging is one of the most commonly used methods for suicide in both sexes worldwide.In a number of countries,hanging mortality has increased over the last decades.Nevertheless,there is a scarcity of studies that have explored the patterns and trends for mortality of suicide by hanging on global,regional and national levels,as most evaluations are limited to certain populations.AIM To assess the trends of suicide mortality by hanging,strangulation,and suffocation in Serbia,from 1991 to 2020.METHODS This nationwide study,with epidemiological descriptive study design,was carried out based on official data.The age-standardized rates(ASRs,expressed per 100000 persons)were calculated by direct standardization,using the World Standard Population.Mortality trends from suicide by hanging were assessed using the joinpoint regression analysis:The average annual percent change(AAPC)with the corresponding 95%confidence interval(95%CI)was calculated.Age-period-cohort analysis was performed to address the possible underlying reasons for the observed suicide trends.RESULTS Over the 30-year period studied,there were 24340 deaths by hanging(17750 males and 6590 females)in Serbia.In 2020,the ASR of deaths by hanging was 4.5 per 100000 persons in both sexes together(7.6 in males vs 1.7 in females).The trends of suicide mortality by hanging decreased significantly between 1991 and 2020 in both males(AAPC=-1.7%per year;95%CI:-2.0 to-1.4)and females(AAPC=-3.5%per year;95%CI:-3.9 to-3.1).Mortality rates of suicide by hanging had a continuously decreasing tendency in both sexes together in all age groups:The only exception was among males in 40-49 age group,with an increasing trend of suicide by hanging from 1991 to 2011(by+0.3%per year).CONCLUSION The trends in suicide mortality by hanging have been decreasing in Serbia in the last three decades in both sexes,but this was more pronounced in women than in men.Despite the decreasing trends observed in mortality of suicide by hanging,further research is needed for better clarification of trends and help in suicide prevention in the future.
基金the National Natural Science Foundation of China (Grants 51605377 and 81300696)the Natural Science Foundation of Shaanxi Province (Grant 2017JQ5004).
文摘Hanging-drop method has been widely used to fabricate three-dimensional (3D) in vitro tissue models due to its advantages such as being easy to perform, inexpensive, and permitting precise control of cell spheroid formation. The geometry of hanging drop may play a critical role on the formation of cell spheroids, which, however, has not been explored. In this study, we developed a modified hanging-drop platform that enables the production of cell spheroids in a high-throughput manner by controlling hanging drop geometry with defined spreading ring. The surface tension force is proportional to the spreading ring and gravitational force is determined by the droplet volume, and the geometry can be determined by the balance between surface tension and gravity.β-TC-6 cell spheroids with optimized diameters were fabricated as 3D in vitro islet models. The models show morphology similar to primary islets and have functionality that more closely resembles primary islets than two-dimensional cell culture. The developed platform holds great potential for engineering well-controlled in vitro tissue models for various applications such as physiological and pathological studies, drug screening, as well as transplantation for treatment purpose.
基金Basic Science Research Foundation of Institute of Engineering Mechanics, China Earthquake Administration (2006B07) Natural Science Foundation of Heilongjiang Province (E2007-13)Joint Seismological Science Foundation of China (C07025)
文摘Root-mean-square distance Drms with characteristic of weighted-average is introduced in this article firstly. Drms can be used to capture the general proximity of a site to a dipping fault plane comparing with the rupture distance Drup and the seismogenic distance Dseis. Then, using Drup, Dseis and Drms, the hanging wall/footwall effects on the peak ground acceleration (PGA) during the 1999 Chi-Chi earthquake are evaluated by regression analysis. The logarithm residual shows that the PGA on hanging wall is much greater than that on footwall at the same Drup or Dseis when the Drup or Dseis is used as site-to-source distance measure. In contrast, there is no significant difference between the PGA on hanging wall and that on footwall at the same Drms when Drms is used. This result confirms that the hanging wall/footwall effect is mainly a geometric effect caused by the asymmetry of dipping fault. Therefore, the hanging wall/footwall effect on the near-fault ground motions can be ignored in the future attenuation analysis if the root-mean-square distance Drms is used as the site-to-source distance measure.
文摘Cibaliung Underground Gold Mine applies cut and fill mining method to optimize ore production and maintain underground stability. Existing 5 m × 5 m height and width of stope geometry has a potential new design to increase production of gold due to variety of thick ore, however serious shotcrete failures often occur in hanging wall decline. This paper aims to find out the relationship between stope design and stability of hanging wall decline. The analysis conducted in this study is based on underground characteristics and geological condition of Cibaliung area. The impact of stope design on decline stability was analyzed by using numerical methods. The impact factors such as different rock mass, size of stope, and distance between stope and hanging wall decline were used in the analysis of underground stability especially stability on hanging wall decline.
文摘Based on the Han Dynasty stone reliefs unearthed in the ancient Xuzhou area,a kind of pavilion above the water—hanging waterside pavilion(Xuan Shui Xie,built above the water with stilts and brackets as the base and seeming hanging on the water)was systematically collected and classifi ed into 2 major categories:single-bracket column hanging waterside pavilion and multiple-bracket column hanging waterside pavilion.The research showed that hanging waterside pavilion got ride of traditional pavilion’s reliance on rammed earth foundation.In addition to working as a connection part,brackets,or bucket arches(Dougong)had also tremendous load-bearing capacity,straight and curved brackets co-formed a giant multiple bracket system,staircases and one-side hanging structure were used to support the upper construction,so that a new towering but light wooden structure hanging above the water was created,which was an innovation of bracket technique of the Han Dynasty,and also a creative design in the development of wooden architecture.
文摘As a person in the petroleum industry,I worked in Bangladesh for many years.When I lived in the Legation Quarter in Dacca,the capital of Bangladesh,I liked wearing the most popular flip-flops.When I lived in the Customs Logistics Park in Chittagong,I liked being bound with an exotic headscarf.When I was stationed at a well site in Sylhet,I liked dressing in my dazzling"Petroleum Red".During my period working in Bangladesh,I was impressed by the crowded roads of the country.Especially,
文摘This paper retrospectively analyzes the complicated rectovaginal fistula in 2 cases after the successful treatment. Through literatures collected, with the difficulties and contradictions of the treatment of complicated rectovaginal fistula as the starting point, the currently used hanging line method, the advantage and deficiency of surgical treatment in recent years, and the change of treatment method were reviewed. We wish to explore which current treatments could be a better choice.
文摘A national flag is the representative symbol of a country.It is displayed on many occasions in society.A code comprised of three letters is delegated to each countryand is often used in
文摘This series of hanging charts is easy to read and contains the common symbols used in daily life.The complete series of hanging charts includes traffic,safety and environmental protection symbols。
文摘Objective: Conservative restoration principles that accurately reduce plaque accumulation are the goals of restorative dentistry. Correct and perfect restoration has an important effect on health of periodontious tissues. This study was done to compare the methods of oral hygiene, over hanged removal and restoration changing on periodontal health and alveolar bone height of restored mandible molars with proximal over hanged amalgam. Materials and Methods: All patients with over hanged amalgam restoration were randomly divided into three groups. In order to survey the effect of over hanged removal on gingival and bleeding indices and alveolar bone height adjacent to mandible molar teeth, these indices were determined according to recurrent radio-graphical early signs of alveolar bone loss during three months. Sixty patients, 20 in each group, were studied. In the group I, overhang was removed with ultrasonic scale or bur and oral hygiene methods as usual were continued. In the group II, over-hang was left, but plaque controls as the prophylaxis and flossing were done. In the group III, restoration was changed and oral hygiene was done as usual. Plaque index and bleeding index were measured at the beginning of study and three months later. Findings: Statistical analysis of studied indices at the beginning and three months after treatment using paired test has shown significant differences in gingival inflammation, in all groups. Statistical analysis of indices using Tukey HSD test has also shown significant differences in decreasing gingival inflammation measure when the over hanged restoration was removed. While there is no difference between the other two groups. Conclusion: The results showed that plaque control causes subsidence of gingival inflammation and increasing of alveolar bone support, and it is more effective when the over hanged restoration is changed.
基金This work was financially supported by FP7 of the EU through the project‘Body on a chip’,ICT-FET-296257the ERC Advanced Grant‘NeuroCMOS’(contract 267351)as well as by an individual Ambizione Grant 142440 from the Swiss National Science Foundation for Olivier Frey.
文摘Microfluidics is becoming a technology of growing interest for building microphysiological systems with integrated read-out functionalities.Here we present the integration of enzyme-based multi-analyte biosensors into a multi-tissue culture platform for‘body-on-a-chip’applications.The microfluidic platform is based on the technology of hanging-drop networks,which is designed for the formation,cultivation,and analysis of fluidically interconnected organotypic spherical three-dimensional(3D)microtissues of multiple cell types.The sensor modules were designed as small glass plug-ins featuring four platinum working electrodes,a platinum counter electrode,and an Ag/AgCl reference electrode.They were placed directly into the ceiling substrate from which the hanging drops that host the spheroid cultures are suspended.The electrodes were functionalized with oxidase enzymes to enable continuous monitoring of lactate and glucose through amperometry.The biosensors featured high sensitivities of 322±41 nA mM^(−1) mm^(−2) for glucose and 443±37 nA mM^(−1) mm^(−2) for lactate;the corresponding limits of detection were below 10μM.The proposed technology enabled tissue-size-dependent,real-time detection of lactate secretion from single human colon cancer microtissues cultured in the hanging drops.Furthermore,glucose consumption and lactate secretion were monitored in parallel,and the impact of different culture conditions on the metabolism of cancer microtissues was recorded in real-time.
基金N.R.acknowledges financial support from a doctoral scholarship(FRQNT 199851)from the Fonds de Recherche du Québec—Nature et Technologies.R.L.S.acknowledges financial support from a scholarship(LCF/BQ/EU19/11710046)from the“La Caixa”FoundationWe acknowledge the help of Christian Lohasz,ETH Zürich,with the cell culture,maintenance,and characterization of HCT116 spheroids.We acknowledge the help of Flavio Bonanini,ETH Zürich,with the determination of cell seeding densities and control of HCT116 growth dynamicsWe acknowledge the help of Stefan Strebel,University of Basel,and Felix Franke,ETH Zürich,with developing the mathematical models.
文摘As 3D in vitro tissue models become more pervasive,their built-in nutrient,metabolite,compound,and waste gradients increase biological relevance at the cost of analysis simplicity.Investigating these gradients and the resulting metabolic heterogeneity requires invasive and time-consuming methods.An alternative is using electrochemical biosensors and measuring concentrations around the tissue model to obtain size-dependent metabolism data.With our hanging-dropintegrated enzymatic glucose biosensors,we conducted current measurements within hanging-drop compartments hosting spheroids formed from the human colorectal carcinoma cell line HCT116.We developed a physics-based mathematical model of analyte consumption and transport,considering(1)diffusion and enzymatic conversion of glucose to form hydrogen peroxide(H_(2)O_(2))by the glucose-oxidase-based hydrogel functionalization of our biosensors at the microscale;(2)H_(2)O_(2)oxidation at the electrode surface,leading to amperometric H_(2)O_(2)readout;(3)glucose diffusion and glucose consumption by cancer cells in a spherical tissue model at the microscale;(4)glucose and H_(2)O_(2)transport in our hangingdrop compartments at the macroscale;and(5)solvent evaporation,leading to glucose and H_(2)O_(2)upconcentration.Our model relates the measured currents to the glucose concentrations generating the currents.The low limit of detection of our biosensors(0.4±0.1μM),combined with our current-fitting method,enabled us to reveal glucose dynamics within our system.By measuring glucose dynamics in hanging-drop compartments populated by cancer spheroids of various sizes,we could infer glucose distributions within the spheroid,which will help translate in vitro 3D tissue model results to in vivo.
文摘Adaptive higher-order finite element methods(hp-FEM)are well known for their potential of exceptionally fast(exponential)convergence.However,most hp-FEM codes remain in an academic setting due to an extreme algorithmic complexity of hp-adaptivity algorithms.This paper aims at simplifying hpadaptivity for H(curl)-conforming approximations by presenting a novel technique of arbitrary-level hanging nodes.The technique is described and it is demonstrated numerically that it makes adaptive hp-FEM more efficient compared to hp-FEM on regular meshes and meshes with one-level hanging nodes.
文摘Hanging wall syncline and central dome are special extension structures, developing over the hanging wall in an extensional ramp-flat fault. Under the condition that the flat is sub-horizontal, the hanging wall syncline is separated from the half graben by the central dome. And on the dome forms an erosional surface. Both sediments in the half graben and erosional surface on the top of the central dome extended over the dome and entered into the hanging wall syncline with extension going on. Meanwhile, those having entered were overlapped by new sedimentary layers in the hanging wall syncline, so that there is a together-threaded, diachronic unconformity to form in the same epoch stratum. The layers in the hanging wall syncline also have an attribute of migrating laterally and getting tilted with extension. There is no sedimentation on the central dome. But sediments, which came from the half graben, got thicker over the dome in extension.
基金The research was supported by the National Science Foundation of China(Grant No.31971795)and Priority Academic Program Development of Jiangsu Higher Education Institutions(Grant No.PAPD-2018-87)The authors are grateful to the National Science Foundation of China.The first author thanks the China Scholarship Council(2017GXZ026592)for providing 36 months scholarship for studying in China.The first author would like to thank his mother and wife Sidra for their moral support。
文摘Mechanical damage induced by vibration during harvesting and post-harvest handling could decrease the quality,quantity,and shelf life of the fresh grape cluster.Usually,fresh grape clusters are harvested by gripping and cutting from the main rachis in the present robotic harvesting system,then transported towards the basket during post-harvest handling.However,serious cluster vibration and corresponding berry falling may occur during the robotic transportation of hanging grape clusters.Therefore,this study was designed to perform experimental and theoretical hanging force analysis to explore the vibration mechanism of hanging grape clusters during robotic transportation.A lead screw lathe with an attached linear actuator was used to investigate the effects of four different speeds(0.4,0.6,0.8,1.0 m/s)with four acceleration levels(6,8,10,12 m/s2)on the vibration of the hanging grape cluster.By the experiments,the peak hanging force of the grape cluster at the start,constant speed,and stop phase of the actuator was recorded using a single axis force sensor,and the cluster’s swing angle was measured with a digital camera.The experimental results showed a linear relationship between the swing angle and hanging force of the cluster at the start and stop phase of the actuator.The multi-stage cluster’s vibration during robotic transportation was observed,and the behavior of cycled damping after a sudden stop of the actuator was found.The simulated results of hanging force of grape cluster in damping phase were agreed with experimental results with R2 more than 0.90 at an optimum acceleration of 10 m/s2.To conclude,this research provides theoretical basics for understanding the complex vibration mechanism of the hanging cluster fruits during speedy robotic transportation operations with low-loss of berry drop both on industrial and farm levels.
文摘Violence against the neck can result in a range of macromorphological and micromorphological findings.However,the forensic relevance of the carotid sinus in cases of violence against the neck remains controversial.In this follow-up study of 22 cases of suicidal and accidental strangulations,carotid bifurcations were examined histologically for morphological changes implying direct trauma,including haemorrhage and immunohistochemical expression of heat-shock proteins 27,60,and 70 and aquaporin-3.These cases were compared with a control group(82 cases)without neck compression or head trauma and with variable causes of death.No relevant histopathological findings implying direct trauma of the carotid bifurcation were found.No cases showed positive aquaporin-3 staining and only five cases showed positive heat-shock protein-27 staining,all of which were hangings.Without massive trauma of the carotid bifurcation,histological alterations cannot be expected.Without signs of rapid death,findings of acute circulatory failure,macromorphological and micromorphological findings of neck compression,and reliable markers indicating relevant impact on the carotid bifurcation the diagnosis of a lethal reflex cannot be verified.
