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Constituents of the anti-asthma herbal formula ASHMI^(TM) synergistically inhibit IL-4 and IL-5 secretion by murine Th2 memory cells,and eotaxin by human lung fibroblasts in vitro 被引量:15
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作者 Bolleddula Jayaprakasam Nan Yang +4 位作者 Ming-Chun Wen Rong Wang Joseph Goldfarb Hugh Sampson Xiu-Min Li 《Journal of Integrative Medicine》 SCIE CAS CSCD 2013年第3期195-205,共11页
OBJECTIVE: Anti-asthma herbal medicine intervention (ASHMITM), a combination of three tradi- tional Chinese medicinal herbs developed in our laboratory, has demonstrated efficacy in both mouse models of allergic as... OBJECTIVE: Anti-asthma herbal medicine intervention (ASHMITM), a combination of three tradi- tional Chinese medicinal herbs developed in our laboratory, has demonstrated efficacy in both mouse models of allergic asthma, and a double-blind placebo-controlled clinical trial in patients with asthma. This study was designed to determine if the anti-inflammatory effects of individual herbal constituents of ASHMITM exhibited synergy. METHODS: Effects of ASHMI and its components aqueous extracts of Lingzhi (Ganoderma lucidum), Kushen (Sophora flavescens) and Gancao (Glycyrrhiza uralensis), on Th2 cytokine secretion by murine memory Th2 cells (D10.G4.1) and eotaxin-1 secretion by human lung fibroblast (HLF-1) cells were determined by measuring levels in culture supernatants by enzyme- linked immunosorbent assay. Potential synergistic effects were determined by computing interaction indices from concentration-effect curve parameters. RESULTS: Individual Lingzhi, Kushen and Gancao extracts and ASHMI (the combination of individual extracts) inhibited production of interleukin (IL)-4 and IL-5 by murine memory Th2 cells and eotaxin-1 production by HLF-1 cells. The mean 25%-inhibitory-concentration (IC2s) values (mg/mL) forASHMI, Lingzhi, Kushen and Gancao for IL-4 production were 30.9, 79.4, 123, and 64.6, respectively; for IL-5 production were 30.2, 263, 123.2 and 100, respectively; for eotaxin-1 were 13.2, 16.2, 30.2, and 25.1, respectively. The IC50values (mg/mL) for ASHMI, Lingzhi, Kushen and Gancao for IL-4 production were 158.5, 239.9, 446.7, and 281.8, respectively; for eotaxin-1 were 38.1, 33.1, 100, and 158.5, respectively. The interaction indices of ASHMI constituents at IC25 were 0.35 for IL-4, 0.21 for IL-5 and 0.59 for eotaxin-l. The interaction indices at IC^0 values were 0.50 for IL-4 and 0.62 for eotaxin-1 inhibition. Inhibition of IL-5 did not reach IC^0 values. All interaction indices were below 1 which indicated synergy. CONCLUSION: By comparing the interaction index values, we find that constituents in ASHMITM synergistically inhibited eotaxin-1 production as well as Th2 cytokine production. 展开更多
关键词 medicine Chinese traditional medicine herbal plant extracts anti-asthma herbal medicine intervention (ASHMI) anti-asthmatic agents chemokine CCL11 INTERLEUKIN-4 interleukin -5 in vitro
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Glycyrrhiza uralensis flavonoids inhibit brain microglial cell TNF-α secretion,p-IκB expression,and increase brain-derived neurotropic factor(BDNF)secretion 被引量:3
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作者 Sangita P.Patil Changda Liu +2 位作者 Joseph Alban Nan Yang Xiu-Min Li 《Journal of Traditional Chinese Medical Sciences》 2014年第1期28-37,共10页
Objective:Asthma sufferers exhibit high prevalence of anxiety/depression.Elevated tumor-necrosis factor-alpha(TNF-α)levels in peripheral system and central nervous system(CNS)are associated with anxiety/depression,wh... Objective:Asthma sufferers exhibit high prevalence of anxiety/depression.Elevated tumor-necrosis factor-alpha(TNF-α)levels in peripheral system and central nervous system(CNS)are associated with anxiety/depression,whereas brain-derived neurotropic factor(BDNF)has anti-depressant effects.An anti-asthma herbal medicine intervention ASHMI inhibits peripheral TNF-αsecretion in an animal model of asthma.We hypothesize that ASHMI and its compounds may have modulatory effects on CNS TNF-αand BDNF production.We sought to determine the effect of ASHMI and individual herb constituents on brain microglial cell TNF-αproduction,and identify the active compounds that suppress TNF-αand increase BDNF.Methods:BV-2 mouse microglial cells were pre-treated with ASHMI or extracts of Ganoderma lucidum(G.lucidum),Sophora flavescens Ait(S.flavescens),and Glycyrrhiza uralensis Fischer(G.uralensis),the herbal constituents in ASHMI,or individual compounds isolated from G.uralensis at different concentrations and then stimulated with LPS.TNF-αlevels in culture supernatants were measured by ELISA.The effect of active compounds on NFkB signaling pathway and on BDNF production were determined by western blotting and ELISA,respectively.Results:ASHMI produced dose-dependent inhibition of TNF-αsecretion by cultured-mousemicroglia BV2 cells.Of the three herb extracts in ASHMI,only G.uralensis significantly and dosedependently inhibited TNF-αproduction.Among the 5 flavonoids isolated from G.uralensis,isoliquiritigeninwas themost effective.Isoliquiritigenin suppression of TNF-αproduction was associated with attenuation of p-NF-kB expression,andwas accompanied by increased BDNF secretion.Conclusion:ASHMI and its effective flavonoid,isoliquiritigenin,inhibited TNF-αproduction by LPS stimulatedmicroglial cells and elevated BDNF levels,which may prove to have anti-CNS inflammatory and anti-anxiety effects. 展开更多
关键词 Anti-asthma herbal medicine intervention Anxiety and asthma FLAVONOIDS TNF-Α BDNF
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A traditional Chinese herbal medicine compound preparation versus interventional therapy after resection of small hepatocellular carcinoma:22-year follow-up 被引量:8
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作者 孙振 梁水庭 +7 位作者 翟笑枫 郎庆波 周庆辉 岳小强 贺佳 徐静 朱役 凌昌全 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2012年第2期156-163,共8页
OBJECTIVE:To compare the long-term effectiveness of compound Ruanjianhugan(RJH)tablets and interventional therapy(IT) in patients after resection of small hepatocellular carcinoma(HCC).METHODS:A retrospective study wa... OBJECTIVE:To compare the long-term effectiveness of compound Ruanjianhugan(RJH)tablets and interventional therapy(IT) in patients after resection of small hepatocellular carcinoma(HCC).METHODS:A retrospective study was conducted in 399 patients after resection of small HCC who were admitted between January 1987 and December 2008 in the Department of Hepatobiliary Surgery and Center of Minimlly Invasive Surgery,First Affiliated Hospital of Guangxi Medical University.Four groups were based on different therapy modes:a TCM-only(TCMO) group,a TCM combined with interventional therapy(TCM-IT) group,an interventional therapy-only(ITO) group,and a simple operation(SO) group.Prognostic factors were correlated with overall survival(OS) and OS rates were calculated with the Kaplan-Meier method,and multivariate analyses for factors affecting survival were evaluated by the Cox proportional hazard model.RESULTS:The median OS was 151.20 months in the TCM-IT group,43.87 months in the ITO group,and 20.77 months in the SO group.All survival rates of the TCMO group were higher than those of the other three groups(>50%).The 5-,10-,and 15-year OS in the TCMO and ITO patients were 83.94%,45.50%,and 71.22% and 33.34%,55.58%,and 9.26%,respectively(risk ratio,0.209;95% confidence interval,0.126-0.347;P=0.000).Multivariate analysis revealed that the independent risk factors were therapy mode(P=0.000),sex(P=0.005),family history(P=0.011),TNM Classification of Malignant Tumor staging(P=0.000),medical care-seeking behavior(P=0.021),and maximum diameter(P=0.030).CONCLUSION:Long-term oral use of compound RJH tablets may improve OS for small HCC after resection compared with IT. 展开更多
关键词 Hepatocellular carcinoma interventional therapy herbal therapy Traditional Chinese medicine Prognostic factors
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