Objective:To observe the changes in insulin resistance in patients with gestational diabetes mellitus(GDM)based on the detection of serum microRNA-372-3p and glucose transporter protein 4(GLUT4)levels.Methods:We condu...Objective:To observe the changes in insulin resistance in patients with gestational diabetes mellitus(GDM)based on the detection of serum microRNA-372-3p and glucose transporter protein 4(GLUT4)levels.Methods:We conducted a retrospective cohort study of 42 patients who were diagnosed with GDM and hospitalized in our hospital during the period from January 2017 to December 2021 and another 42 patients who had normal pregnancy during the same period by collecting their clinical data.We analyzed their serum microRNA expression profiles and miR-372-3p levels to study the relationship between GDM and insulin resistance.Results:The relative expression of miR-372-3p in the serum of patients in the GDM group was significantly higher than that of patients in the control group,but the GLUT 4 level of the GDM group was significantly lower than that of the control group(P<0.05).Compared with the control group,the GDM group had significantly higher levels of fasting blood glucose(FBG),fasting insulin(FINS),2-hour postprandial blood glucose(2h-BG),total cholesterol(TC),triglyceride(TG),and homeostatic model assessment for insulin resistance(HOMA-IR)index but significantly lower homeostasis model assessment ofβ-cell function(HOMA-β)index(P<0.05).The relative expression of miR-372-3p in serum was independently and positively correlated with HOMA-IR,while the level of GLUT4 was independently and negatively correlated with HOMA-IR(P<0.05).Conclusion:Glycosylated hemoglobin test in the early stages of pregnancy(12–13 weeks of gestation)is important to ensure the health of pregnant women and fetuses.The screening and intervention for elevated glucose in pregnant women act as a guideline for the treatment of GDM.Patients with insulin resistance and related complications such as hyperinsulinemia and hypoglycemia should be given priority.展开更多
目的:检测miR-372-3p在膀胱癌组织和转染miR-372-3p mimics膀胱癌细胞中的表达,研究miR-372-3p对膀胱癌5637和T24细胞增殖、迁移与侵袭的影响。方法:采集2016年3月至2017年1月武汉中心医院收治的6例膀胱癌患者癌及癌旁组织(距离肿瘤边缘...目的:检测miR-372-3p在膀胱癌组织和转染miR-372-3p mimics膀胱癌细胞中的表达,研究miR-372-3p对膀胱癌5637和T24细胞增殖、迁移与侵袭的影响。方法:采集2016年3月至2017年1月武汉中心医院收治的6例膀胱癌患者癌及癌旁组织(距离肿瘤边缘>5 cm),q PCR检测膀胱癌及癌旁组织miR-372-3p表达。采用脂质体转染法将miR-372-3p mimics或者miRNC转入膀胱癌5637和T24细胞。用q PCR和Western blotting检测转染miR-372-3p mimics和miR-NC膀胱癌细胞miR-372-3p和ATAD2 m RNA和蛋白E-cadherin、N-cadherin蛋白的表达,流式细胞术检测细胞周期分布,MTT法和集落形成实验检测细胞增殖和集落形成能力,划痕实验和Transwell侵袭实验检测细胞的迁移和侵袭能力。结果:膀胱癌组织miR-372-3p m RNA的表达显著低于癌旁组织(0.65±0.56 vs 1.76±0.34,P<0.01)。和转染miR-NC膀胱癌细胞相比,转染miR-372-3p mimics膀胱癌细胞的miR-372-3p m RNA表达显著增加,ATAD2 m RNA和蛋白的表达显著降低,E-cadherin蛋白表达上调,N-cadherin蛋白表达下调,细胞周期明显阻滞,细胞集落形成和增殖能力显著降低,细胞迁移数和侵袭数减少。结论:miR-372-3p的低表达可能与膀胱癌的发生发展有关,其通过靶向调控ATAD2抑制膀胱癌细胞的增殖、迁移和侵袭能力,可能成为膀胱癌生物治疗的新靶标。展开更多
基金supported by the following projects:Youth Science and Technology Fund of Affiliated Hospital of Hebei University(2017Q024)Baoding City Science and Technology Plan Project(2041zf295),and Hebei University Medical Subject Cultivation Project(2022b03).
文摘Objective:To observe the changes in insulin resistance in patients with gestational diabetes mellitus(GDM)based on the detection of serum microRNA-372-3p and glucose transporter protein 4(GLUT4)levels.Methods:We conducted a retrospective cohort study of 42 patients who were diagnosed with GDM and hospitalized in our hospital during the period from January 2017 to December 2021 and another 42 patients who had normal pregnancy during the same period by collecting their clinical data.We analyzed their serum microRNA expression profiles and miR-372-3p levels to study the relationship between GDM and insulin resistance.Results:The relative expression of miR-372-3p in the serum of patients in the GDM group was significantly higher than that of patients in the control group,but the GLUT 4 level of the GDM group was significantly lower than that of the control group(P<0.05).Compared with the control group,the GDM group had significantly higher levels of fasting blood glucose(FBG),fasting insulin(FINS),2-hour postprandial blood glucose(2h-BG),total cholesterol(TC),triglyceride(TG),and homeostatic model assessment for insulin resistance(HOMA-IR)index but significantly lower homeostasis model assessment ofβ-cell function(HOMA-β)index(P<0.05).The relative expression of miR-372-3p in serum was independently and positively correlated with HOMA-IR,while the level of GLUT4 was independently and negatively correlated with HOMA-IR(P<0.05).Conclusion:Glycosylated hemoglobin test in the early stages of pregnancy(12–13 weeks of gestation)is important to ensure the health of pregnant women and fetuses.The screening and intervention for elevated glucose in pregnant women act as a guideline for the treatment of GDM.Patients with insulin resistance and related complications such as hyperinsulinemia and hypoglycemia should be given priority.
文摘目的:检测miR-372-3p在膀胱癌组织和转染miR-372-3p mimics膀胱癌细胞中的表达,研究miR-372-3p对膀胱癌5637和T24细胞增殖、迁移与侵袭的影响。方法:采集2016年3月至2017年1月武汉中心医院收治的6例膀胱癌患者癌及癌旁组织(距离肿瘤边缘>5 cm),q PCR检测膀胱癌及癌旁组织miR-372-3p表达。采用脂质体转染法将miR-372-3p mimics或者miRNC转入膀胱癌5637和T24细胞。用q PCR和Western blotting检测转染miR-372-3p mimics和miR-NC膀胱癌细胞miR-372-3p和ATAD2 m RNA和蛋白E-cadherin、N-cadherin蛋白的表达,流式细胞术检测细胞周期分布,MTT法和集落形成实验检测细胞增殖和集落形成能力,划痕实验和Transwell侵袭实验检测细胞的迁移和侵袭能力。结果:膀胱癌组织miR-372-3p m RNA的表达显著低于癌旁组织(0.65±0.56 vs 1.76±0.34,P<0.01)。和转染miR-NC膀胱癌细胞相比,转染miR-372-3p mimics膀胱癌细胞的miR-372-3p m RNA表达显著增加,ATAD2 m RNA和蛋白的表达显著降低,E-cadherin蛋白表达上调,N-cadherin蛋白表达下调,细胞周期明显阻滞,细胞集落形成和增殖能力显著降低,细胞迁移数和侵袭数减少。结论:miR-372-3p的低表达可能与膀胱癌的发生发展有关,其通过靶向调控ATAD2抑制膀胱癌细胞的增殖、迁移和侵袭能力,可能成为膀胱癌生物治疗的新靶标。
文摘目的·分析人类mi R-223-3p(hsa-miR-223-3p)的靶基因及其参与的生物学过程,并寻找与糖尿病相关的生物标志物。方法·利用starBase数据库筛选hsa-miR-223-3p靶基因,并对其行基因本体数据库(Gene Ontology,GO)富集分析、京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genome,KEGG)和Reactome通路分析。通过构建蛋白-蛋白相互作用(proteinprotein interaction,PPI)网络获得中枢基因,筛选最有意义的模块,并利用Venn图对糖尿病相关的中枢基因进行分析。结果·共筛选出870个hsa-miR-223-3p靶基因。GO、KEGG和Reactome富集分析显示,靶基因主要与RNA聚合酶Ⅱ启动子的调节、细胞对胰岛素刺激的反应、RNA结合等相关,且主要富集于胰岛素分泌、泛素介导的蛋白水解、雌激素依赖型基因表达等通路。PPI网络共得31个中枢基因,且中枢基因PRKACB参与胰岛素分泌通路;共筛选出3个最有意义的基因模块,其中模块1参与泛素介导的蛋白水解作用,模块2参与RNA转运和细胞周期,模块3参与内吞作用。结论·Hsa-miR-223-3p可能通过靶基因参与多种生物学过程,中枢基因PRKACB或可为糖尿病发生机制的探索提供帮助。
