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Human antigen R mediated post-transcriptional regulation of inhibitors of apoptosis proteins in pancreatic cancer 被引量:1
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作者 Ausra Lukosiute-Urboniene Aldona Jasukaitiene +3 位作者 Giedre Silkuniene Vidmantas Barauskas Antanas Gulbinas Zilvinas Dambrauskas 《World Journal of Gastroenterology》 SCIE CAS 2019年第2期205-219,共15页
AIM To determine the association of human antigen R(HuR) and inhibitors of apoptosis proteins(IAP1, IAP2) and prognosis in pancreatic cancer.METHODS Protein and mRNA expression levels of IAP1, IAP2 and HuR in pancreat... AIM To determine the association of human antigen R(HuR) and inhibitors of apoptosis proteins(IAP1, IAP2) and prognosis in pancreatic cancer.METHODS Protein and mRNA expression levels of IAP1, IAP2 and HuR in pancreatic ductal adenocarcinoma(PDAC) were compared with normal pancreatic tissue. The correlations among IAP1/IAP2 and HuR as well as their respective correlations with clinicopathological parameters were analyzed. The Kaplan-Meier method and log-rank tests were used for survival analysis. Immunoprecipitation assay was performed to demonstrate HuR binding to IAP1, IAP2 mRNA. PANC1 cells were transfected with either anti-HuR siRNA or control siRNA for 72 h and quantitative reverse transcription polymerase chain reaction(RT-PCR), western blot analysis was carried out.RESULTS RT-PCR analysis revealed that HuR, IAP1, IAP2 mRNA expression were accordingly 3.3-fold, 5.5-fold and 8.4 higher in the PDAC when compared to normal pancreas(P < 0.05). Expression of IAP1 was positively strongly correlated with HuR expression(P < 0.05, r = 0.783). Western blot analysis confirmed RTPCR results. High IAP1 expression, tumor resection status, T stage, lymph-node metastases, tumor differentiation grade, perineural and lymphatic invasion were identified as significant factors for shorter survival in PDAC patients(P < 0.05).Immunohistological analysis showed that HuR was mainly expressed in the ductal cancer cell's nucleus and less so in cytoplasm. RNA immunoprecipitation analysis confirmed IAP1 and IAP2 post-transcriptional regulation by HuR protein. Following siHuR transfection, IAP1 mRNA and protein levels were decreased, however IAP2 expression levels were increased.CONCLUSION HuR mediated overexpression of IAP1 significantly correlates with poor outcomes and early progression of pancreatic cancer. Further studies are needed to assess the underlying mechanisms. 展开更多
关键词 PANCrEATIC cancer Inhibitors of apoptosis PrOTEINS human antigen r posttranscriptional regulation
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Relationship between Fas/ FasL expression and apoptosis of colon adenocarcinoma cell lines 被引量:15
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作者 Zhi Hai Peng Tong Hai Xing +1 位作者 Guo Qiang Qiu Hua Mei Tang Shanghai No. 1 People’s Hospital, Shanghai 200080, China 《World Journal of Gastroenterology》 SCIE CAS CSCD 2001年第1期88-92,共5页
INTRODUCTIONFas/ FasL system has been identified as a keymediator of apoptosis in tumor cells[1-4]. Theoccurrence and development of neoplasm are closelyrelated to apoptosis[5-7] Most chemotherapeuticdrugs kill cancer... INTRODUCTIONFas/ FasL system has been identified as a keymediator of apoptosis in tumor cells[1-4]. Theoccurrence and development of neoplasm are closelyrelated to apoptosis[5-7] Most chemotherapeuticdrugs kill cancer cells mainly by inducingapoptosis[8-14].' 展开更多
关键词 Adenocarcinoma Colonic Neoplasms Antibiotics Antineoplastic antigens CD95 Antimetabolites Antineoplastic Antineoplastic Agents APOPTOSIS Cisplatin EPIrUBICIN Flow Cytometry Fluorouracil gene expression regulation Neoplastic humans Membrane Glycoproteins Mitomycins research Support Non-U.S. Gov't Tumor Cells Cultured
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带FLAG标签小鼠HuR真核表达载体的构建及其生物学功能的鉴定 被引量:3
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作者 李涛 于文燕 +3 位作者 欧小利 刘爱华 梅柱中 姜勇 《解放军医学杂志》 CAS CSCD 北大核心 2011年第4期352-355,共4页
目的构建人抗原R(HuR)的真核表达载体,观察其在NIH3T3细胞中的表达并初步分析其生物学功能。方法提取NIH3T3细胞总RNA并反转录为cDNA,以cDNA为模板PCR扩增得到小鼠HuR编码序列,酶切后克隆至pcDNA3.1-FLAG载体;重组载体经PCR、酶切、测... 目的构建人抗原R(HuR)的真核表达载体,观察其在NIH3T3细胞中的表达并初步分析其生物学功能。方法提取NIH3T3细胞总RNA并反转录为cDNA,以cDNA为模板PCR扩增得到小鼠HuR编码序列,酶切后克隆至pcDNA3.1-FLAG载体;重组载体经PCR、酶切、测序鉴定正确后瞬时转染NIH3T3细胞,采用Western blotting分析HuR在细胞中的表达,并采用Real-time PCR检测HuR过表达对DUSP1mRNA水平的影响。结果成功构建了重组质粒pcDNA3.1-HuR-FLAG,Western blotting显示该质粒能在NIH3T3细胞中高效表达,Real-time PCR结果表明HuR过表达可提高细胞内DUSP1基因的mRNA水平。结论成功构建了带FLAG标签的HuR真核表达载体,该载体能在NIH3T3细胞中有效表达,并具有相应的生物学功能,为深入研究肿瘤细胞中HuR对DUSP1基因表达的调控机制奠定基础。 展开更多
关键词 人抗原r 基因表达 基因转录后调控
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HuR蛋白在卵巢浆液性肿瘤组织中的表达及其意义 被引量:1
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作者 王晓娜 成丽 《河北医药》 CAS 2010年第15期2006-2007,共2页
目的探讨人抗原R(human antigen R,HuR)在卵巢浆液性肿瘤组织中的表达及其临床意义。方法利用免疫组化法和图像分析技术检测10例正常卵巢组织,20例卵巢浆液性瘤,32例卵巢浆液性癌中HuR蛋白的表达。结果正常卵巢组织、卵巢浆液性肿瘤组... 目的探讨人抗原R(human antigen R,HuR)在卵巢浆液性肿瘤组织中的表达及其临床意义。方法利用免疫组化法和图像分析技术检测10例正常卵巢组织,20例卵巢浆液性瘤,32例卵巢浆液性癌中HuR蛋白的表达。结果正常卵巢组织、卵巢浆液性肿瘤组织中均有HuR蛋白表达,HuR蛋白在卵巢癌中的表达较卵巢瘤及正常组织中增强,两两比较差异均有统计学意义(P<0.05)。有淋巴结转移的卵巢浆液性癌中HuR蛋白表达高于无淋巴结转移(P<0.05)。结论卵巢浆液性肿瘤的发生发展与HuR的过表达密切相关。 展开更多
关键词 Hur蛋白 卵巢肿瘤 肿瘤基因表达调控
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