[Objective] The aim of the research was to study the effect of the ice bath time after heat shock and the incubation time on the transformation efficacy,and to establish a simple and quick transformation method.[Metho...[Objective] The aim of the research was to study the effect of the ice bath time after heat shock and the incubation time on the transformation efficacy,and to establish a simple and quick transformation method.[Method]Competent cells were prepared with two buffer solutions;with the ice bath time after heat shock and the recovery time as the variables,the relationship between these two factors and transformation efficacy was studied.[Result]The transformation efficacy was the best when the ice bath time was 2 min and the recovery time was 30 or 40 min;when the ice bath time and the recovery time was 0 min,a certain amount of transformants still could be obtained.[Conclusion]The ice bath time after heat shock and the recovery time had certain impact on transformation efficacy,but they were not the decisive factors.Therefore,in the general transformation experiment,these two steps could be omitted.展开更多
基金Supported by Foundation for Returned Scholars of Hebei Province(2010)Research Fund for the Doctoral Program of Hebei Normal University(L2009B13)~~
文摘[Objective] The aim of the research was to study the effect of the ice bath time after heat shock and the incubation time on the transformation efficacy,and to establish a simple and quick transformation method.[Method]Competent cells were prepared with two buffer solutions;with the ice bath time after heat shock and the recovery time as the variables,the relationship between these two factors and transformation efficacy was studied.[Result]The transformation efficacy was the best when the ice bath time was 2 min and the recovery time was 30 or 40 min;when the ice bath time and the recovery time was 0 min,a certain amount of transformants still could be obtained.[Conclusion]The ice bath time after heat shock and the recovery time had certain impact on transformation efficacy,but they were not the decisive factors.Therefore,in the general transformation experiment,these two steps could be omitted.
