Background Physical entity interactions in mixed reality(MR)environments aim to harness human capabilities in manipulating physical objects,thereby enhancing virtual environment(VEs)functionality.In MR,a common strate...Background Physical entity interactions in mixed reality(MR)environments aim to harness human capabilities in manipulating physical objects,thereby enhancing virtual environment(VEs)functionality.In MR,a common strategy is to use virtual agents as substitutes for physical entities,balancing interaction efficiency with environmental immersion.However,the impact of virtual agent size and form on interaction performance remains unclear.Methods Two experiments were conducted to explore how virtual agent size and form affect interaction performance,immersion,and preference in MR environments.The first experiment assessed five virtual agent sizes(25%,50%,75%,100%,and 125%of physical size).The second experiment tested four types of frames(no frame,consistent frame,half frame,and surrounding frame)across all agent sizes.Participants,utilizing a head mounted display,performed tasks involving moving cups,typing words,and using a mouse.They completed questionnaires assessing aspects such as the virtual environment effects,interaction effects,collision concerns,and preferences.Results Results from the first experiment revealed that agents matching physical object size produced the best overall performance.The second experiment demonstrated that consistent framing notably enhances interaction accuracy and speed but reduces immersion.To balance efficiency and immersion,frameless agents matching physical object sizes were deemed optimal.Conclusions Virtual agents matching physical entity sizes enhance user experience and interaction performance.Conversely,familiar frames from 2D interfaces detrimentally affect interaction and immersion in virtual spaces.This study provides valuable insights for the future development of MR systems.展开更多
The paper presents the change in grain-size composition of lignite under cyclic freezing-thawing (FTC) and wetting-drying (WDC). The article shows that in the spring and autumn periods the lignites can be subjected to...The paper presents the change in grain-size composition of lignite under cyclic freezing-thawing (FTC) and wetting-drying (WDC). The article shows that in the spring and autumn periods the lignites can be subjected to repeated freezing-thawing and wetting-drying, which determines the possibility of changing their grain-size composition and structure. Experimental studies in laboratory conditions on the influence of cyclic freezing-thawing (FTC) and wetting-drying (WDC) on the quality indicators of lignites have been carried out, their granulometric (fractional) composition has been studied. Freezing-thawing cycle conditions are as follows (FTC): minimum exposure temperature: -20°C;maximum: +5°C;relative humidity: 30%;number of processing cycles: 3. Wetting-drying cycles are as follows (WDC): drying temperatures are +20, +40, +60, +80°C, drying time 90 minutes, the coals are further subjected to rain (soaking) for a period of water saturation to humidity of 30% - 40% and dry again. The number of wetting-drying cycles is 3 times. The tests have revealed the destructive effects of FTC and WDC on the samples of lower metamorphic grade coal, and the cycles of wet-dry lead to the much higher yield of fine sizes (-6+0;-13+0 mm) than the cycles of freeze-thaw. Furthermore, it is found that the increase in the yield of fines depends on the heating temperature: coal disintegration proceeds more intensively at a higher temperature of drying.展开更多
Common problems in engineering projects that involve artificial ground freezing of soil or rock include inadequate thickness,strength and continuity of artificial frozen walls.It is difficult to evaluate the freezing ...Common problems in engineering projects that involve artificial ground freezing of soil or rock include inadequate thickness,strength and continuity of artificial frozen walls.It is difficult to evaluate the freezing state using only a few thermometer holes at fixed positions or with other existing approaches.Here we report a novel experimental design that investigates changes in ultrasonic properties(received waveform,wave velocity V_(p),wave amplitude,frequency spectrum,centroid frequency f_(c),kurtosis of the frequency spectrum KFS,and quality factor Q)measured during upward freezing,compared with those during uniform freezing,in order to determine the freezing state in 150 mm cubic blocks of Ardingly sandstone.Water content,porosity and density were estimated during upward freezing to ascertain water migration and changes of porosity and density at different stages.The period of receiving the wave increased substantially and coda waves changed from loose to compact during both upward and uniform freezing.The trend of increasing V_(p) can be divided into three stages during uniform freezing.During upward freezing,V_(p) increased more or less uniformly.The frequency spectrum could be used as a convenient and rapid method to identify different freezing states of sandstone(unfrozen,upward frozen,and uniformly frozen).The continuous changes in reflection coefficient r_(φ),refraction coefficient t_(φ) and acoustic impedance field are the major reason for larger reflection and refraction during upward freezing compared with uniform freezing.Wave velocity V_(p),wave amplitude A_(h),centroid frequency f_(c) and quality factor Q were adopted as ultrasonic parameters to evaluate quantitatively the temperature T of uniformly frozen sandstone,and their application within a radar chart is recommended.Determination of V_(p) provides a convenient method to evaluate the freezing state and calculate the cryofront height and frozen section thickness of upward frozen sandstone,with accuracies of 73.37%-99.23%.展开更多
Cold acclimation is a complex biological process leading to the development of freezing tolerance in plants.In this study,we demonstrated that cold-induced expression of protease inhibitor FmASP in a Citrus-relative s...Cold acclimation is a complex biological process leading to the development of freezing tolerance in plants.In this study,we demonstrated that cold-induced expression of protease inhibitor FmASP in a Citrus-relative species kumquat[Fortunella margarita(Lour.)Swingle]contributes to its freezing tolerance by minimizing protein degradation.Firstly,we found that only cold-acclimated kumquat plants,despite extensive leaf cellular damage during freezing,were able to resume their normal growth upon stress relief.To dissect the impact of cold acclimation on this anti-freezing performance,we conducted protein abundance assays and quantitative proteomic analysis of kumquat leaves subjected to cold acclimation(4◦C),freezing treatment(−10◦C)and post-freezing recovery(25◦C).FmASP(Against Serine Protease)and several non-specific proteases were identified as differentially expressed proteins induced by cold acclimation and associated with stable protein abundance throughout the course of low-temperature treatment.FmASP was further characterized as a robust inhibitor of multiple proteases.In addition,heterogeneous expression of FmASP in Arabidopsis confirmed its positive role in freezing tolerance.Finally,we proposed a working model of FmASP and illustrated how this extracellular-localized protease inhibitor protects proteins from degradation,therebymaintaining essential cellular function for post-freezing recovery.These findings revealed the important role of protease inhibition in freezing response and provide insights on how this role may help develop new strategies to enhance plant freezing tolerance.展开更多
The soil freezing and thawing process affects soil physical properties,such as heat conductivity,heat capacity,and hydraulic conductivity in frozen ground regions,and further affects the processes of soil energy,hydro...The soil freezing and thawing process affects soil physical properties,such as heat conductivity,heat capacity,and hydraulic conductivity in frozen ground regions,and further affects the processes of soil energy,hydrology,and carbon and nitrogen cycles.In this study,the calculation of freezing and thawing front parameterization was implemented into the earth system model of the Chinese Academy of Sciences(CAS-ESM)and its land component,the Common Land Model(CoLM),to investigate the dynamic change of freezing and thawing fronts and their effects.Our results showed that the developed models could reproduce the soil freezing and thawing process and the dynamic change of freezing and thawing fronts.The regionally averaged value of active layer thickness in the permafrost regions was 1.92 m,and the regionally averaged trend value was 0.35 cm yr–1.The regionally averaged value of maximum freezing depth in the seasonally frozen ground regions was 2.15 m,and the regionally averaged trend value was–0.48 cm yr–1.The active layer thickness increased while the maximum freezing depth decreased year by year.These results contribute to a better understanding of the freezing and thawing cycle process.展开更多
Potato(Solanum tuberosum L.) is the fourth largest food crop in the world. Low temperatures cause serious damage to potato plants every year, and freezing tolerance has become a hot spot in potato research. Galactinol...Potato(Solanum tuberosum L.) is the fourth largest food crop in the world. Low temperatures cause serious damage to potato plants every year, and freezing tolerance has become a hot spot in potato research. Galactinol synthase(GolS) is a key enzyme in the synthesis of raffinose family oligosaccharides(RFOs), and plays an important role in the response of plants to abiotic stress. In this study, the ScGolS1 gene from Solanum commersonii was cloned and introduced into the S. tuberosum cultivars 'Atlantic' and 'Desiree' via Agrobacterium-mediated transformation. Phenotyping assays showed that overexpression of ScGolS1 could significantly improve freezing tolerance in transgenic potato plants.Further physiological and biochemical experiments showed that the transgenic lines had lower relative conductivity, malondialdehyde content,and 3,3-diaminobenzidine staining and a higher plant survival rate compared with wild type(WT) under cold stress. Moreover, the C-repeat binding factors(CBF1, CBF2 and CBF3), the downstream cold-responsive genes COR413 and COR47, and the ethylene-responsive factor(ERF)transcription factor genes ERF3, ERF4 and ERF6, which function in the ethylene signaling pathway, were all induced by freezing treatment and expressed at higher levels in the ScGolS1 overexpression lines compared with WT. Besides, the expression of some genes such as MIPS, STS and RS from the RFO metabolic pathway was up-regulated under cold stress, resulting in changes in the content of some soluble sugars. This indicated that ScGolS1 overexpression altered the sugar composition and enhanced freezing tolerance in transgenic potato by inducing the ethylene and CBF signaling pathways. These results provided theoretical support and genetic resources for freezing tolerance breeding in potato.展开更多
AIM:To evaluate the diagnostic value of panoramic immersion B-scan ultrasonography(Pano-immersion B-scan,PIB)in complex retinal detachment(RD),persistent hyperplastic primary vitreous(PHPV)and intraocular tumors.METHO...AIM:To evaluate the diagnostic value of panoramic immersion B-scan ultrasonography(Pano-immersion B-scan,PIB)in complex retinal detachment(RD),persistent hyperplastic primary vitreous(PHPV)and intraocular tumors.METHODS:The clinical data of 44 patients collected from May 2012 to December 2019 in Chinese PLA General Hospital was retrospectively studied.All of these patients underwent PIB of the eye,because it was difficult to diagnose by routine ocular fundus examination,conventional ultrasound or/and ultrasonic biomicroscope(UBM)due to opacity of refractive media,pupillary occlusion,large involvement or special location of the lesion.The imaging features of difficult cases in PIB were analyzed.The diagnosis accuracy rating of PIB were evaluated and contrasted with conventional ultrasound or UBM by the standard of intraoperative diagnosis or/and pathological results.RESULTS:According to intraoperative diagnosis or pathological results as gold standard,among the 44 cases,there were 19 cases missed diagnosis,misdiagnosed or difficult-to-diagnose by conventional ultrasound or UBM,including 4 cases of long-standing RD difficult to diagnose,4 cases misdiagnosed,and 11 cases incompletely observed or miss diagnosed.The diagnostic accuracy rate of PIB and conventional ultrasound or UBM were 100%(44/44)and 56.82%(25/44),and the sensitivity of them were 100%and 56.82%.All the patients underwent PIB and were diagnosed as RD(15 cases),retinal and choroidal detachment(4 cases),subchoroidal hematocele(1 case),vitreous opacity and/or organic membrane formation(4 cases),PHPV(12 cases),iris and/or ciliary body tumors(3 cases),and choroidal tumors(6 cases).According to the intraoperative diagnosis or pathological results,the diagnostic coincidence rate of PIB was 100%,which was significantly higher than conventional ultrasound and UBM.CONCLUSION:PIB can help to accurately diagnose complex RD,PHPV,and intraocular masses with special location or/and excessive size.It has important diagnostic value for patients with equivocal findings at conventional ultrasound examination.展开更多
Super-resolution imaging with superlens has been one of the fundamental research topics. Unfortunately, the resolution of superlens is inevitably restrained by material loss. To address the problem, we introduce the s...Super-resolution imaging with superlens has been one of the fundamental research topics. Unfortunately, the resolution of superlens is inevitably restrained by material loss. To address the problem, we introduce the solid immersion mechanism into the slab superlens and the cylindrical superlens. The proposed solid immersion slab superlens(SISSL) and the solid immersion cylindrical superlens(SICSL) can improve the resolution by converting evanescent wave to propagating wave using high refractive index materials. From the perspective of applications, the cylindrical superlens with finite cross section and the ability of magnification or demagnification has more advantages than the slab superlens. Therefore,we focus on demonstrating analytically the super-resolution imaging of SICSL. Due to the impedance mismatching caused by solid immersion mechanism, the whispering gallery modes(WGMs) are excited between SICSL and the air interface.We clarify the excitation conditions of WGMs and analyze their influence on the imaging quality of SICSL. The SISSL and SICSL may pave a way to apply in lithography technique and real-time biomolecular imaging in future.展开更多
The calculation of frost heaving with ice lens formation is still not standard for construction projects using artificial ground freezing(AGF).In fine-grained material,ice lenses may initiate and lead to significant h...The calculation of frost heaving with ice lens formation is still not standard for construction projects using artificial ground freezing(AGF).In fine-grained material,ice lenses may initiate and lead to significant heaving at the ground surface,which should be considered in advance.However,the complex processes during ice lens formation are still not fully understood and difficult to capture in a simple approach.In the past,the semi-analytical approach of Konrad and Morgenstern used one soil constant,the“segregation potential(SP)”.It has been mainly and most successfully applied to the heave calculation of natural-induced soil freezing in cold regions.Its application to AGF has been so far unsuccessful.To solve this,a new semi-analytical approach is presented in this paper.It includes AGF conditions such as bottom-up freezing,temperature gradients to reach great freezing velocities,and a distinction between two freezing states.One is the freezing-up state until a certain frost body thickness is reached(thermal transient state),and the other is a holding phase where the frost body thickness is kept constant(thermal quasi-steady state).To test its ability,the results are applied to another freezing direction,the top-down freezing.The new approach is validated using two different frost-susceptible soils and,in total,50 frost heave tests.In the thermal transient region,where the SP is applicable,the two semi-analytical approaches are compared,showing improved performance of the current method by about 15%.展开更多
Since the 1990s,the Qinghai–Tibetan Plateau(QTP)has experienced a strikingly warming and wetter climate that alters the thermal and hydrological properties of frozen ground.A positive correlation between the warming ...Since the 1990s,the Qinghai–Tibetan Plateau(QTP)has experienced a strikingly warming and wetter climate that alters the thermal and hydrological properties of frozen ground.A positive correlation between the warming and thermal degradation in permafrost or seasonally frozen ground(SFG)has long been recognized.Still,a predictive relationship between historical wetting under warming climate conditions and frozen ground has not yet been well demonstrated,despite the expectation that it will become even more important because precipitation over the QTP has been projected to increase continuously in the near future.This study investigates the response of the thermal regime to historical wetting in both permafrost and SFG areas and examines their relationships separately using the Community Land Surface Model version 4.5.Results show that wetting before the 1990s across the QTP mainly cooled the permafrost body in the arid and semiarid zones,with significant correlation coefficients of 0.60 and 0.48,respectively.Precipitation increased continually at the rate of 6.16 mm decade–1 in the arid zone after the 1990s but had a contrasting warming effect on permafrost through a significant shortening of the thawing duration within the active layer.However,diminished rainfall in the humid zone after the 1990s also significantly extended the thawing duration of SFG.The relationship between the ground thawing index and precipitation was significantly negatively correlated(−0.75).The dual effects of wetting on the thermal dynamics of the QTP are becoming critical because of the projected increases in future precipitation.展开更多
The characteristics of the raindrop size distribution(DSD)during regional freezing rain(FR)events that occur throughout the phase change(from liquid to solid)are poorly understood due to limited observations.We invest...The characteristics of the raindrop size distribution(DSD)during regional freezing rain(FR)events that occur throughout the phase change(from liquid to solid)are poorly understood due to limited observations.We investigate the evolution of microphysical parameters and the key formation mechanisms of regional FR using the DSDs from five disdrometer sites in January 2018 in the Jianghan Plain(JHP)of Central China.FR is identified via the size and velocity distribution measured from a disdrometer,the discrete Fréchet distancemethod,surface temperature,human observations,and sounding data.With the persistence of precipitation,the emergence of graupel or snowflakes significantly reduces the proportion of FR.The enhancement of this regional FR event is mainly dominated by the increase in the number concentration of raindrops but weakly affected by the diameters.To improve the accuracy of quantitative precipitation estimation for the FR event,a modified second-degree polynomial relation between the shapeμand slopeΛof gamma DSDs is derived,and a new Z-R(radar reflectivity to rain rate)relationship is developed.The mean values of mass-weighted mean diameters(D_(m))and generalized intercepts(lgN_(w))in FR are close to the stratiform results in the northern region of China.Both the melting of tiny-rimed graupels and large-dry snowflakes are a response to the formation of this regional FR process in the JHP,dominated by the joint influence of the physical mechanism of warm rain,vapor deposition,and aggregation/riming coupled with the effect of weak convective motion in some periods.展开更多
In most areas of China, affected by the environment of low temperature and high humidity, the wind speed sensor and wind direction sensor are frozen and cannot output data in autumn, winter or the alternation of winte...In most areas of China, affected by the environment of low temperature and high humidity, the wind speed sensor and wind direction sensor are frozen and cannot output data in autumn, winter or the alternation of winter and spring. In order to solve the freezing situation of the wind sensor, this paper designs a new type of antifreeze wind speed sensor. After meteorology performance testing and field observation tests, the correlation coefficient of the observation data is demonstrated, and the data curve is fitted. The result shows the sensor is stable, and has a good antifreeze effect, the data output is reliable.展开更多
The term“freezing”refers to an innate defensive reaction characterized by the sudden cessation of all movements(Stote&Fanselow,2004;Eilam,2005).The freezing effect involving a police officer exposes him to a ris...The term“freezing”refers to an innate defensive reaction characterized by the sudden cessation of all movements(Stote&Fanselow,2004;Eilam,2005).The freezing effect involving a police officer exposes him to a risk to himself and others.Materials and Methods:400 anonymous questionnaires administered to law enforcement personnel in Switzerland were evaluated with the aim of testing their knowledge about the freezing phenomenon.In addition,semi-structured interviews involving police personnel were conducted.The authors also analyzed two cases of police officers who had experienced freezing.Results and Conclusions:From the results obtained,it emerges that freezing is not known to most of the police officers interviewed.Therefore,specific and innovative training seems to be necessary.The authors described the brain mechanisms that are involved in freezing.Innovative integrated training modules have been proposed by applying some neuroscientific knowledge,particularly using the potential of mirror neurons.The authors propose a strategy for supporting police officers who have experienced an episode of freezing.The ultimate goal is to provide more scientific tools to protect police officers and civilians.展开更多
With the advent of the information age,augmented reality technology can enhance the sense of reality in the virtual world and immerse people in the real and virtual world.People have always been interested in virtual ...With the advent of the information age,augmented reality technology can enhance the sense of reality in the virtual world and immerse people in the real and virtual world.People have always been interested in virtual space or augmented reality technology,especially in the face of historical development trends.Museums have always been open to the public,they are not just a collection,but also an exhibition for the public.Through analyzing different museums,it is found that museums with augmented reality technology exhibitions are non-profit museums for the purpose of emotional experience.In order to find out the immersion factors of virtual reality in museums,on the basis of previous research,this paper divides immersion in augmented reality into story immersion,five-sense experience immersion,and spatial interaction immersion.Through the analysis of different museums,it is found that immersion through five-sense experience is the most commonly used method when all display media are included.This method provides educational content for museum visitors and projects virtual presentations through monitors and portable IT devices,thereby increasing visitors’viewing pleasure.展开更多
Purpose–This paper aims to study the impacts of groundwater seepage on artificial freezing process of gravel strata,the temperature field characteristics of the strata,and the strata process,closure time and thicknes...Purpose–This paper aims to study the impacts of groundwater seepage on artificial freezing process of gravel strata,the temperature field characteristics of the strata,and the strata process,closure time and thickness evolution mechanism of the frozen wall.Design/methodology/approach–In this paper several laboratory model tests were conducted,considering different groundwater seepage rate.Findings–The results show that there is a significant coupling effect between the cold diffusion of artificial freezing pipes and groundwater seepage;when there is no seepage,temperature fields upstream and downstream of the gravel strata are symmetrically distributed,and the thickness of the frozen soil column/frozen wall is consistent during artificial freezing;groundwater seepage causes significant asymmetry in the temperature fields upstream and downstream of the gravel strata,and the greater the seepage rate,the more obvious the asymmetry;the frozen wall closure time increases linearly with the increase in the groundwater seepage rate,and specifically,the time length under seepage rate of 5.00 m d1 is 3.2 times longer than that under no seepage;due to the erosion from groundwater seepage,the thickness of the upstream frozen wall decreases linearly with the seepage velocity,while that of the downstream frozen wall increases linearly,resulting in a saddle-shaped frozen wall.Originality/value–The research results are beneficial to the optimum design and risk control of artificial freezing process in gravel strata.展开更多
Purpose–This study purposes to study the influence of artificial freezing on the liquefaction characteristics of Nanjing sand,as well as its mechanism.Design/methodology/approach–was studied through dynamic triaxial...Purpose–This study purposes to study the influence of artificial freezing on the liquefaction characteristics of Nanjing sand,as well as its mechanism.Design/methodology/approach–was studied through dynamic triaxial tests by means of the GDS dynamic triaxial system on Nanjing sand extensively discovered in the middle and lower reaches of the Yangtze River under seismic load and metro train vibration load,respectively,and potential hazards of the two loads to the freezing construction of Nanjing sand were also identified in the tests.Findings–The results show that under both seismic load and metro train vibration load,freeze-thaw cycles will significantly reduce the stiffness and liquefaction resistance of Nanjing sand,especially in the first freezethaw cycle;the more freeze-thaw cycles,the worse structural behaviors of silty-fine sand,and the easier to liquefy;freeze-thaw cycles will increase the sensitivity of Nanjing sand’s dynamic pore pressure to dynamic load response;the lower the freezing temperature and the effective confining pressure,the worse the liquefaction resistance of Nanjing sand after freeze-thaw cycles;compared to the metro train vibration load,the seismic load in Nanjing is potentially less dangerous to freezing construction of Nanjing sand.Originality/value–The research results are helpful to the construction of the artificial ground freezing of the subway crossing passage in the lower reaches of the Yangtze River and to ensure the construction safety of the subway tunnel and its crossing passage.展开更多
基金the Strategic research and consulting project of Chinese Academy of Engineering(2023-HY-14).
文摘Background Physical entity interactions in mixed reality(MR)environments aim to harness human capabilities in manipulating physical objects,thereby enhancing virtual environment(VEs)functionality.In MR,a common strategy is to use virtual agents as substitutes for physical entities,balancing interaction efficiency with environmental immersion.However,the impact of virtual agent size and form on interaction performance remains unclear.Methods Two experiments were conducted to explore how virtual agent size and form affect interaction performance,immersion,and preference in MR environments.The first experiment assessed five virtual agent sizes(25%,50%,75%,100%,and 125%of physical size).The second experiment tested four types of frames(no frame,consistent frame,half frame,and surrounding frame)across all agent sizes.Participants,utilizing a head mounted display,performed tasks involving moving cups,typing words,and using a mouse.They completed questionnaires assessing aspects such as the virtual environment effects,interaction effects,collision concerns,and preferences.Results Results from the first experiment revealed that agents matching physical object size produced the best overall performance.The second experiment demonstrated that consistent framing notably enhances interaction accuracy and speed but reduces immersion.To balance efficiency and immersion,frameless agents matching physical object sizes were deemed optimal.Conclusions Virtual agents matching physical entity sizes enhance user experience and interaction performance.Conversely,familiar frames from 2D interfaces detrimentally affect interaction and immersion in virtual spaces.This study provides valuable insights for the future development of MR systems.
文摘The paper presents the change in grain-size composition of lignite under cyclic freezing-thawing (FTC) and wetting-drying (WDC). The article shows that in the spring and autumn periods the lignites can be subjected to repeated freezing-thawing and wetting-drying, which determines the possibility of changing their grain-size composition and structure. Experimental studies in laboratory conditions on the influence of cyclic freezing-thawing (FTC) and wetting-drying (WDC) on the quality indicators of lignites have been carried out, their granulometric (fractional) composition has been studied. Freezing-thawing cycle conditions are as follows (FTC): minimum exposure temperature: -20°C;maximum: +5°C;relative humidity: 30%;number of processing cycles: 3. Wetting-drying cycles are as follows (WDC): drying temperatures are +20, +40, +60, +80°C, drying time 90 minutes, the coals are further subjected to rain (soaking) for a period of water saturation to humidity of 30% - 40% and dry again. The number of wetting-drying cycles is 3 times. The tests have revealed the destructive effects of FTC and WDC on the samples of lower metamorphic grade coal, and the cycles of wet-dry lead to the much higher yield of fine sizes (-6+0;-13+0 mm) than the cycles of freeze-thaw. Furthermore, it is found that the increase in the yield of fines depends on the heating temperature: coal disintegration proceeds more intensively at a higher temperature of drying.
基金supported by the National Natural Science Foundation of China(Grant Nos.51804157,51774183,and 11702094)the University of Sussex,UK.Both are gratefully acknowledged.
文摘Common problems in engineering projects that involve artificial ground freezing of soil or rock include inadequate thickness,strength and continuity of artificial frozen walls.It is difficult to evaluate the freezing state using only a few thermometer holes at fixed positions or with other existing approaches.Here we report a novel experimental design that investigates changes in ultrasonic properties(received waveform,wave velocity V_(p),wave amplitude,frequency spectrum,centroid frequency f_(c),kurtosis of the frequency spectrum KFS,and quality factor Q)measured during upward freezing,compared with those during uniform freezing,in order to determine the freezing state in 150 mm cubic blocks of Ardingly sandstone.Water content,porosity and density were estimated during upward freezing to ascertain water migration and changes of porosity and density at different stages.The period of receiving the wave increased substantially and coda waves changed from loose to compact during both upward and uniform freezing.The trend of increasing V_(p) can be divided into three stages during uniform freezing.During upward freezing,V_(p) increased more or less uniformly.The frequency spectrum could be used as a convenient and rapid method to identify different freezing states of sandstone(unfrozen,upward frozen,and uniformly frozen).The continuous changes in reflection coefficient r_(φ),refraction coefficient t_(φ) and acoustic impedance field are the major reason for larger reflection and refraction during upward freezing compared with uniform freezing.Wave velocity V_(p),wave amplitude A_(h),centroid frequency f_(c) and quality factor Q were adopted as ultrasonic parameters to evaluate quantitatively the temperature T of uniformly frozen sandstone,and their application within a radar chart is recommended.Determination of V_(p) provides a convenient method to evaluate the freezing state and calculate the cryofront height and frozen section thickness of upward frozen sandstone,with accuracies of 73.37%-99.23%.
基金sponsored by the National Natural Science Foundation of China(No.31200963)the Key Project of Hunan Provincial Education Department(No.18A091).
文摘Cold acclimation is a complex biological process leading to the development of freezing tolerance in plants.In this study,we demonstrated that cold-induced expression of protease inhibitor FmASP in a Citrus-relative species kumquat[Fortunella margarita(Lour.)Swingle]contributes to its freezing tolerance by minimizing protein degradation.Firstly,we found that only cold-acclimated kumquat plants,despite extensive leaf cellular damage during freezing,were able to resume their normal growth upon stress relief.To dissect the impact of cold acclimation on this anti-freezing performance,we conducted protein abundance assays and quantitative proteomic analysis of kumquat leaves subjected to cold acclimation(4◦C),freezing treatment(−10◦C)and post-freezing recovery(25◦C).FmASP(Against Serine Protease)and several non-specific proteases were identified as differentially expressed proteins induced by cold acclimation and associated with stable protein abundance throughout the course of low-temperature treatment.FmASP was further characterized as a robust inhibitor of multiple proteases.In addition,heterogeneous expression of FmASP in Arabidopsis confirmed its positive role in freezing tolerance.Finally,we proposed a working model of FmASP and illustrated how this extracellular-localized protease inhibitor protects proteins from degradation,therebymaintaining essential cellular function for post-freezing recovery.These findings revealed the important role of protease inhibition in freezing response and provide insights on how this role may help develop new strategies to enhance plant freezing tolerance.
基金This work was jointly funded by the National Natural Science Foundation of China(Grant Nos.42205168,41830967,and 42175163)the Youth Innovation Promotion Association CAS(2021073)the National Key Scientific and Technological Infrastructure project“Earth System Science Numerical Simulator Facility”(EarthLab).
文摘The soil freezing and thawing process affects soil physical properties,such as heat conductivity,heat capacity,and hydraulic conductivity in frozen ground regions,and further affects the processes of soil energy,hydrology,and carbon and nitrogen cycles.In this study,the calculation of freezing and thawing front parameterization was implemented into the earth system model of the Chinese Academy of Sciences(CAS-ESM)and its land component,the Common Land Model(CoLM),to investigate the dynamic change of freezing and thawing fronts and their effects.Our results showed that the developed models could reproduce the soil freezing and thawing process and the dynamic change of freezing and thawing fronts.The regionally averaged value of active layer thickness in the permafrost regions was 1.92 m,and the regionally averaged trend value was 0.35 cm yr–1.The regionally averaged value of maximum freezing depth in the seasonally frozen ground regions was 2.15 m,and the regionally averaged trend value was–0.48 cm yr–1.The active layer thickness increased while the maximum freezing depth decreased year by year.These results contribute to a better understanding of the freezing and thawing cycle process.
基金supported by the National Natural Science Foundation of China (Grant No. 31561143006)China Agriculture Research System (Grant No. CARS-9)the Chinese Academy of Agricultural Sciences Innovation Project (Grant No. CAASASTIP-IVFCAAS)。
文摘Potato(Solanum tuberosum L.) is the fourth largest food crop in the world. Low temperatures cause serious damage to potato plants every year, and freezing tolerance has become a hot spot in potato research. Galactinol synthase(GolS) is a key enzyme in the synthesis of raffinose family oligosaccharides(RFOs), and plays an important role in the response of plants to abiotic stress. In this study, the ScGolS1 gene from Solanum commersonii was cloned and introduced into the S. tuberosum cultivars 'Atlantic' and 'Desiree' via Agrobacterium-mediated transformation. Phenotyping assays showed that overexpression of ScGolS1 could significantly improve freezing tolerance in transgenic potato plants.Further physiological and biochemical experiments showed that the transgenic lines had lower relative conductivity, malondialdehyde content,and 3,3-diaminobenzidine staining and a higher plant survival rate compared with wild type(WT) under cold stress. Moreover, the C-repeat binding factors(CBF1, CBF2 and CBF3), the downstream cold-responsive genes COR413 and COR47, and the ethylene-responsive factor(ERF)transcription factor genes ERF3, ERF4 and ERF6, which function in the ethylene signaling pathway, were all induced by freezing treatment and expressed at higher levels in the ScGolS1 overexpression lines compared with WT. Besides, the expression of some genes such as MIPS, STS and RS from the RFO metabolic pathway was up-regulated under cold stress, resulting in changes in the content of some soluble sugars. This indicated that ScGolS1 overexpression altered the sugar composition and enhanced freezing tolerance in transgenic potato by inducing the ethylene and CBF signaling pathways. These results provided theoretical support and genetic resources for freezing tolerance breeding in potato.
文摘AIM:To evaluate the diagnostic value of panoramic immersion B-scan ultrasonography(Pano-immersion B-scan,PIB)in complex retinal detachment(RD),persistent hyperplastic primary vitreous(PHPV)and intraocular tumors.METHODS:The clinical data of 44 patients collected from May 2012 to December 2019 in Chinese PLA General Hospital was retrospectively studied.All of these patients underwent PIB of the eye,because it was difficult to diagnose by routine ocular fundus examination,conventional ultrasound or/and ultrasonic biomicroscope(UBM)due to opacity of refractive media,pupillary occlusion,large involvement or special location of the lesion.The imaging features of difficult cases in PIB were analyzed.The diagnosis accuracy rating of PIB were evaluated and contrasted with conventional ultrasound or UBM by the standard of intraoperative diagnosis or/and pathological results.RESULTS:According to intraoperative diagnosis or pathological results as gold standard,among the 44 cases,there were 19 cases missed diagnosis,misdiagnosed or difficult-to-diagnose by conventional ultrasound or UBM,including 4 cases of long-standing RD difficult to diagnose,4 cases misdiagnosed,and 11 cases incompletely observed or miss diagnosed.The diagnostic accuracy rate of PIB and conventional ultrasound or UBM were 100%(44/44)and 56.82%(25/44),and the sensitivity of them were 100%and 56.82%.All the patients underwent PIB and were diagnosed as RD(15 cases),retinal and choroidal detachment(4 cases),subchoroidal hematocele(1 case),vitreous opacity and/or organic membrane formation(4 cases),PHPV(12 cases),iris and/or ciliary body tumors(3 cases),and choroidal tumors(6 cases).According to the intraoperative diagnosis or pathological results,the diagnostic coincidence rate of PIB was 100%,which was significantly higher than conventional ultrasound and UBM.CONCLUSION:PIB can help to accurately diagnose complex RD,PHPV,and intraocular masses with special location or/and excessive size.It has important diagnostic value for patients with equivocal findings at conventional ultrasound examination.
基金Project supported by the National Key Research and Development Program of China (Grant No. 2020YFA0710100)the National Natural Science Foundation of China (Grant Nos. 92050102 and 11874311)the Fundamental Research Funds for the Central Universities (Grant Nos. 20720220033 and 20720200074)。
文摘Super-resolution imaging with superlens has been one of the fundamental research topics. Unfortunately, the resolution of superlens is inevitably restrained by material loss. To address the problem, we introduce the solid immersion mechanism into the slab superlens and the cylindrical superlens. The proposed solid immersion slab superlens(SISSL) and the solid immersion cylindrical superlens(SICSL) can improve the resolution by converting evanescent wave to propagating wave using high refractive index materials. From the perspective of applications, the cylindrical superlens with finite cross section and the ability of magnification or demagnification has more advantages than the slab superlens. Therefore,we focus on demonstrating analytically the super-resolution imaging of SICSL. Due to the impedance mismatching caused by solid immersion mechanism, the whispering gallery modes(WGMs) are excited between SICSL and the air interface.We clarify the excitation conditions of WGMs and analyze their influence on the imaging quality of SICSL. The SISSL and SICSL may pave a way to apply in lithography technique and real-time biomolecular imaging in future.
基金supported by the German Research Foundation(DFG)under the project“Investigation and calculation of frost heave considering specific boundary conditions of ground freezing”(Grant No.409760547).
文摘The calculation of frost heaving with ice lens formation is still not standard for construction projects using artificial ground freezing(AGF).In fine-grained material,ice lenses may initiate and lead to significant heaving at the ground surface,which should be considered in advance.However,the complex processes during ice lens formation are still not fully understood and difficult to capture in a simple approach.In the past,the semi-analytical approach of Konrad and Morgenstern used one soil constant,the“segregation potential(SP)”.It has been mainly and most successfully applied to the heave calculation of natural-induced soil freezing in cold regions.Its application to AGF has been so far unsuccessful.To solve this,a new semi-analytical approach is presented in this paper.It includes AGF conditions such as bottom-up freezing,temperature gradients to reach great freezing velocities,and a distinction between two freezing states.One is the freezing-up state until a certain frost body thickness is reached(thermal transient state),and the other is a holding phase where the frost body thickness is kept constant(thermal quasi-steady state).To test its ability,the results are applied to another freezing direction,the top-down freezing.The new approach is validated using two different frost-susceptible soils and,in total,50 frost heave tests.In the thermal transient region,where the SP is applicable,the two semi-analytical approaches are compared,showing improved performance of the current method by about 15%.
基金supported by the National Natural Science Foundation of China (Grant Nos. 41905008, 41975007, and 42075081)the Innovation and Entrepreneurship Training Program for College Students of Chengdu University of Information Technology (CUIT) (202210621003, 202210621039, 202110621015)provided by the Scientific Research Foundation of CUIT (KYTZ202126)
文摘Since the 1990s,the Qinghai–Tibetan Plateau(QTP)has experienced a strikingly warming and wetter climate that alters the thermal and hydrological properties of frozen ground.A positive correlation between the warming and thermal degradation in permafrost or seasonally frozen ground(SFG)has long been recognized.Still,a predictive relationship between historical wetting under warming climate conditions and frozen ground has not yet been well demonstrated,despite the expectation that it will become even more important because precipitation over the QTP has been projected to increase continuously in the near future.This study investigates the response of the thermal regime to historical wetting in both permafrost and SFG areas and examines their relationships separately using the Community Land Surface Model version 4.5.Results show that wetting before the 1990s across the QTP mainly cooled the permafrost body in the arid and semiarid zones,with significant correlation coefficients of 0.60 and 0.48,respectively.Precipitation increased continually at the rate of 6.16 mm decade–1 in the arid zone after the 1990s but had a contrasting warming effect on permafrost through a significant shortening of the thawing duration within the active layer.However,diminished rainfall in the humid zone after the 1990s also significantly extended the thawing duration of SFG.The relationship between the ground thawing index and precipitation was significantly negatively correlated(−0.75).The dual effects of wetting on the thermal dynamics of the QTP are becoming critical because of the projected increases in future precipitation.
基金supported by the National Natural Science Foundation of China(Grant Nos.41875170 and 41675136)the National Key Research and Development Program of China(2018YFC1507201 and 2018YFC1507905)the Guangxi Key Research and Development Program(AB20159013)。
文摘The characteristics of the raindrop size distribution(DSD)during regional freezing rain(FR)events that occur throughout the phase change(from liquid to solid)are poorly understood due to limited observations.We investigate the evolution of microphysical parameters and the key formation mechanisms of regional FR using the DSDs from five disdrometer sites in January 2018 in the Jianghan Plain(JHP)of Central China.FR is identified via the size and velocity distribution measured from a disdrometer,the discrete Fréchet distancemethod,surface temperature,human observations,and sounding data.With the persistence of precipitation,the emergence of graupel or snowflakes significantly reduces the proportion of FR.The enhancement of this regional FR event is mainly dominated by the increase in the number concentration of raindrops but weakly affected by the diameters.To improve the accuracy of quantitative precipitation estimation for the FR event,a modified second-degree polynomial relation between the shapeμand slopeΛof gamma DSDs is derived,and a new Z-R(radar reflectivity to rain rate)relationship is developed.The mean values of mass-weighted mean diameters(D_(m))and generalized intercepts(lgN_(w))in FR are close to the stratiform results in the northern region of China.Both the melting of tiny-rimed graupels and large-dry snowflakes are a response to the formation of this regional FR process in the JHP,dominated by the joint influence of the physical mechanism of warm rain,vapor deposition,and aggregation/riming coupled with the effect of weak convective motion in some periods.
文摘In most areas of China, affected by the environment of low temperature and high humidity, the wind speed sensor and wind direction sensor are frozen and cannot output data in autumn, winter or the alternation of winter and spring. In order to solve the freezing situation of the wind sensor, this paper designs a new type of antifreeze wind speed sensor. After meteorology performance testing and field observation tests, the correlation coefficient of the observation data is demonstrated, and the data curve is fitted. The result shows the sensor is stable, and has a good antifreeze effect, the data output is reliable.
文摘The term“freezing”refers to an innate defensive reaction characterized by the sudden cessation of all movements(Stote&Fanselow,2004;Eilam,2005).The freezing effect involving a police officer exposes him to a risk to himself and others.Materials and Methods:400 anonymous questionnaires administered to law enforcement personnel in Switzerland were evaluated with the aim of testing their knowledge about the freezing phenomenon.In addition,semi-structured interviews involving police personnel were conducted.The authors also analyzed two cases of police officers who had experienced freezing.Results and Conclusions:From the results obtained,it emerges that freezing is not known to most of the police officers interviewed.Therefore,specific and innovative training seems to be necessary.The authors described the brain mechanisms that are involved in freezing.Innovative integrated training modules have been proposed by applying some neuroscientific knowledge,particularly using the potential of mirror neurons.The authors propose a strategy for supporting police officers who have experienced an episode of freezing.The ultimate goal is to provide more scientific tools to protect police officers and civilians.
文摘With the advent of the information age,augmented reality technology can enhance the sense of reality in the virtual world and immerse people in the real and virtual world.People have always been interested in virtual space or augmented reality technology,especially in the face of historical development trends.Museums have always been open to the public,they are not just a collection,but also an exhibition for the public.Through analyzing different museums,it is found that museums with augmented reality technology exhibitions are non-profit museums for the purpose of emotional experience.In order to find out the immersion factors of virtual reality in museums,on the basis of previous research,this paper divides immersion in augmented reality into story immersion,five-sense experience immersion,and spatial interaction immersion.Through the analysis of different museums,it is found that immersion through five-sense experience is the most commonly used method when all display media are included.This method provides educational content for museum visitors and projects virtual presentations through monitors and portable IT devices,thereby increasing visitors’viewing pleasure.
基金supported by the National Natural Science Foundation of China(Grant No.51978426)the NSFC Young Scientists Fund(Grant No.41801277)+1 种基金the Science and Technology Research Program of Hebei Education Department(Grant No.QN2018072)the Program for High-level Talent Fund of Hebei Province(Grant No.A201903010).
文摘Purpose–This paper aims to study the impacts of groundwater seepage on artificial freezing process of gravel strata,the temperature field characteristics of the strata,and the strata process,closure time and thickness evolution mechanism of the frozen wall.Design/methodology/approach–In this paper several laboratory model tests were conducted,considering different groundwater seepage rate.Findings–The results show that there is a significant coupling effect between the cold diffusion of artificial freezing pipes and groundwater seepage;when there is no seepage,temperature fields upstream and downstream of the gravel strata are symmetrically distributed,and the thickness of the frozen soil column/frozen wall is consistent during artificial freezing;groundwater seepage causes significant asymmetry in the temperature fields upstream and downstream of the gravel strata,and the greater the seepage rate,the more obvious the asymmetry;the frozen wall closure time increases linearly with the increase in the groundwater seepage rate,and specifically,the time length under seepage rate of 5.00 m d1 is 3.2 times longer than that under no seepage;due to the erosion from groundwater seepage,the thickness of the upstream frozen wall decreases linearly with the seepage velocity,while that of the downstream frozen wall increases linearly,resulting in a saddle-shaped frozen wall.Originality/value–The research results are beneficial to the optimum design and risk control of artificial freezing process in gravel strata.
基金supported by the National Natural Science Foundation of China(Grant No.U41702299).
文摘Purpose–This study purposes to study the influence of artificial freezing on the liquefaction characteristics of Nanjing sand,as well as its mechanism.Design/methodology/approach–was studied through dynamic triaxial tests by means of the GDS dynamic triaxial system on Nanjing sand extensively discovered in the middle and lower reaches of the Yangtze River under seismic load and metro train vibration load,respectively,and potential hazards of the two loads to the freezing construction of Nanjing sand were also identified in the tests.Findings–The results show that under both seismic load and metro train vibration load,freeze-thaw cycles will significantly reduce the stiffness and liquefaction resistance of Nanjing sand,especially in the first freezethaw cycle;the more freeze-thaw cycles,the worse structural behaviors of silty-fine sand,and the easier to liquefy;freeze-thaw cycles will increase the sensitivity of Nanjing sand’s dynamic pore pressure to dynamic load response;the lower the freezing temperature and the effective confining pressure,the worse the liquefaction resistance of Nanjing sand after freeze-thaw cycles;compared to the metro train vibration load,the seismic load in Nanjing is potentially less dangerous to freezing construction of Nanjing sand.Originality/value–The research results are helpful to the construction of the artificial ground freezing of the subway crossing passage in the lower reaches of the Yangtze River and to ensure the construction safety of the subway tunnel and its crossing passage.
