Objective To investigate the optimal timing of acute kidney injury environment for proliferation and differentiation of mesenchymal stem cells(MSCs).Methods MSCs were co-cultured with kidney homogenate at different is...Objective To investigate the optimal timing of acute kidney injury environment for proliferation and differentiation of mesenchymal stem cells(MSCs).Methods MSCs were co-cultured with kidney homogenate at different ischemia/reperfusion(I/R)time points.The proliferation was reflected by CCK-8assay and cell number counting.The differentiation was reflected by cell shape and CK-18RNA expression.Results After 15dculture,shape of MSCs cultured with kidney homogenate at 72htime point was much more round and ellipsoidal,and the cells had a cobble like appearance.Cell number,CCK-8and CK-18level of MSCs cultured with kidney homogenate at 72htime point was significantly higher comparing with other time points.Conclusions 72hpost I/R was the optimal timing for proliferation and differentiation of MSCs.展开更多
文摘Objective To investigate the optimal timing of acute kidney injury environment for proliferation and differentiation of mesenchymal stem cells(MSCs).Methods MSCs were co-cultured with kidney homogenate at different ischemia/reperfusion(I/R)time points.The proliferation was reflected by CCK-8assay and cell number counting.The differentiation was reflected by cell shape and CK-18RNA expression.Results After 15dculture,shape of MSCs cultured with kidney homogenate at 72htime point was much more round and ellipsoidal,and the cells had a cobble like appearance.Cell number,CCK-8and CK-18level of MSCs cultured with kidney homogenate at 72htime point was significantly higher comparing with other time points.Conclusions 72hpost I/R was the optimal timing for proliferation and differentiation of MSCs.
