AIM: To identify the relationship between the tagging single nucleotide polymorphism sites (tagSNPs) of the Interleukin-18 (IL-18) gene and genetic susceptibility to chronic hepatitis B virus infection in Chinese pati...AIM: To identify the relationship between the tagging single nucleotide polymorphism sites (tagSNPs) of the Interleukin-18 (IL-18) gene and genetic susceptibility to chronic hepatitis B virus infection in Chinese patients. METHODS: Five hundred and one cases of chronic hepatitis B virus (HBV) infection and 301 HBV natural clearance controls were studied. Two tagSNPs in the IL-18 gene (rs1946518A/C and rs574424C/G) were genotyped by the Multiplex Snapshot technique. The genotype and allele frequencies were calculated and analyzed. RESULTS: In the genotypes of rs1946518, the AA type was present at a higher frequency in the patients compared to those in the controls. Odds ratio (OR) of the AA genotype for the comparison with that of the AC and the CC genotype was 1.537 (95% confidence intervals (CI): 1.116-2.218, P = 0.009 < 0.025). In phenotypes, the allele C at rs1946518 was of a significantly lower frequency in the patients with chronic hepatitis B than that in the controls (P = 0.017 < 0.025). OR of the allele A for the comparison with that of the allele C was 1.279 (95% CI: 1.045-1.567). As for the rs574424 genotypes, no significant difference in this genotype distribution or in this allele frequency between the patients and the control subjects was observed. No significant difference in the haplotype frequencies between the patients with chronic hepatitis B and HBV natural clearance individuals was displayed. CONCLUSION: The data suggest that genotype AA and the allele A of the IL-18 at position rs1946518 are closely associated with the resistance to chronic hepatitis B and may be the dangerous gene. However, no statistical association was found between polymorphisms of rs574424 for IL-18 and hepatitis B.展开更多
目的研究血清骨膜蛋白(Periostin)、白细胞介素(IL)-18在支气管肺发育不良(BPD)早产儿中的水平,分析二者与病情严重程度的关系及对BPD的预测价值。方法回顾性选取2019年1月至2022年1月于该院诊治的62例BPD早产儿为BPD组,以同期80例非BP...目的研究血清骨膜蛋白(Periostin)、白细胞介素(IL)-18在支气管肺发育不良(BPD)早产儿中的水平,分析二者与病情严重程度的关系及对BPD的预测价值。方法回顾性选取2019年1月至2022年1月于该院诊治的62例BPD早产儿为BPD组,以同期80例非BPD早产儿为非BPD组。根据病情严重程度,将BPD组患儿分为轻度亚组22例,中度亚组24例和重度亚组16例。采用酶联免疫吸附试验检测血清Periostin、IL-18水平。各临床参数间的相关性采用Pearson相关分析。采用多因素Logistic回归分析BPD发生的影响因素,受试者工作特征(ROC)曲线分析各指标对BPD的预测价值。结果BPD组使用肺泡表面活性物质(PS)、新生儿呼吸窘迫综合征、呼吸暂停、动脉导管未闭占比及血清Periostin、IL-18水平高于非BPD组,机械通气时间、无创呼吸支持时间、住院时间长于非BPD组,而肺功能指标[千克体重潮气量(VT/kg)、达峰时间比(TPTEF/TE)、达峰容积比(VPEF/VE)、50%潮气量时呼气流速(50%TEF)、75%潮气量时呼气流速(75%TEF)]及1、5 min Apgar评分低于非BPD组,差异均有统计学意义(P<0.05)。轻度亚组、中度亚组及重度亚组患儿血清Periostin、IL-18水平依次升高(P<0.05)。血清Periostin、IL-18水平与肺功能指标(VT/kg、50%TEF、75%TEF、TPTEF/TE、VPEF/VE)均呈负相关(P<0.05)。早产儿血清Periostin、IL-18、新生儿呼吸窘迫综合征是影响BPD发生的独立危险因素(P<0.05),使用PS是保护因素(P<0.05)。早产儿血清Periostin、IL-18、新生儿呼吸窘迫综合征是影响BPD病情严重程度的独立危险因素(P<0.05)。血清Periostin、IL-18单独及联合对BPD预测的曲线下面积(95%CI)分别为0.841(0.814~0.899)、0.863(0.820~0.897)、0.922(0.878~0.949),联合预测的灵敏度和特异度分别为0.902、0.825;二者联合对BPD预测的曲线下面积大于各指标单独预测,差异有统计学意义(Z=5.357、4.894,均P<0.001)。结论BPD患儿血清Periostin、IL-18水平升高,二者与BPD病情严重程度及肺功能有关,血清Periostin、IL-18联合对BPD具有较高的预测价值。展开更多
基金Supported by the Grants from the National Natural Science Foundation of China,No.81072342the Foundation of Pre-973 Program Projects,No.2009CB526411
文摘AIM: To identify the relationship between the tagging single nucleotide polymorphism sites (tagSNPs) of the Interleukin-18 (IL-18) gene and genetic susceptibility to chronic hepatitis B virus infection in Chinese patients. METHODS: Five hundred and one cases of chronic hepatitis B virus (HBV) infection and 301 HBV natural clearance controls were studied. Two tagSNPs in the IL-18 gene (rs1946518A/C and rs574424C/G) were genotyped by the Multiplex Snapshot technique. The genotype and allele frequencies were calculated and analyzed. RESULTS: In the genotypes of rs1946518, the AA type was present at a higher frequency in the patients compared to those in the controls. Odds ratio (OR) of the AA genotype for the comparison with that of the AC and the CC genotype was 1.537 (95% confidence intervals (CI): 1.116-2.218, P = 0.009 < 0.025). In phenotypes, the allele C at rs1946518 was of a significantly lower frequency in the patients with chronic hepatitis B than that in the controls (P = 0.017 < 0.025). OR of the allele A for the comparison with that of the allele C was 1.279 (95% CI: 1.045-1.567). As for the rs574424 genotypes, no significant difference in this genotype distribution or in this allele frequency between the patients and the control subjects was observed. No significant difference in the haplotype frequencies between the patients with chronic hepatitis B and HBV natural clearance individuals was displayed. CONCLUSION: The data suggest that genotype AA and the allele A of the IL-18 at position rs1946518 are closely associated with the resistance to chronic hepatitis B and may be the dangerous gene. However, no statistical association was found between polymorphisms of rs574424 for IL-18 and hepatitis B.
文摘目的研究血清骨膜蛋白(Periostin)、白细胞介素(IL)-18在支气管肺发育不良(BPD)早产儿中的水平,分析二者与病情严重程度的关系及对BPD的预测价值。方法回顾性选取2019年1月至2022年1月于该院诊治的62例BPD早产儿为BPD组,以同期80例非BPD早产儿为非BPD组。根据病情严重程度,将BPD组患儿分为轻度亚组22例,中度亚组24例和重度亚组16例。采用酶联免疫吸附试验检测血清Periostin、IL-18水平。各临床参数间的相关性采用Pearson相关分析。采用多因素Logistic回归分析BPD发生的影响因素,受试者工作特征(ROC)曲线分析各指标对BPD的预测价值。结果BPD组使用肺泡表面活性物质(PS)、新生儿呼吸窘迫综合征、呼吸暂停、动脉导管未闭占比及血清Periostin、IL-18水平高于非BPD组,机械通气时间、无创呼吸支持时间、住院时间长于非BPD组,而肺功能指标[千克体重潮气量(VT/kg)、达峰时间比(TPTEF/TE)、达峰容积比(VPEF/VE)、50%潮气量时呼气流速(50%TEF)、75%潮气量时呼气流速(75%TEF)]及1、5 min Apgar评分低于非BPD组,差异均有统计学意义(P<0.05)。轻度亚组、中度亚组及重度亚组患儿血清Periostin、IL-18水平依次升高(P<0.05)。血清Periostin、IL-18水平与肺功能指标(VT/kg、50%TEF、75%TEF、TPTEF/TE、VPEF/VE)均呈负相关(P<0.05)。早产儿血清Periostin、IL-18、新生儿呼吸窘迫综合征是影响BPD发生的独立危险因素(P<0.05),使用PS是保护因素(P<0.05)。早产儿血清Periostin、IL-18、新生儿呼吸窘迫综合征是影响BPD病情严重程度的独立危险因素(P<0.05)。血清Periostin、IL-18单独及联合对BPD预测的曲线下面积(95%CI)分别为0.841(0.814~0.899)、0.863(0.820~0.897)、0.922(0.878~0.949),联合预测的灵敏度和特异度分别为0.902、0.825;二者联合对BPD预测的曲线下面积大于各指标单独预测,差异有统计学意义(Z=5.357、4.894,均P<0.001)。结论BPD患儿血清Periostin、IL-18水平升高,二者与BPD病情严重程度及肺功能有关,血清Periostin、IL-18联合对BPD具有较高的预测价值。
