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白细胞介素-1B-511基因型与慢性牙周炎和冠心病相关性的研究 被引量:4
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作者 张源明 钟良军 +4 位作者 古丽波斯坦.吐尔逊 聂晶 王璇 陈晓涛 封燕 《新疆医科大学学报》 CAS 2004年第3期202-204,共3页
目的 :探讨白细胞介素 - 1B- 5 11(interleukin- 1B- 5 11,IL- 1B- 5 11)基因型与慢性牙周炎 (chronicperiodontitis,CP)和冠心病 (coronary heart disease,CHD)易感性的关系。 方法:采用序列特异引物多聚酶链反应(SSP- PCR)法和多聚酶... 目的 :探讨白细胞介素 - 1B- 5 11(interleukin- 1B- 5 11,IL- 1B- 5 11)基因型与慢性牙周炎 (chronicperiodontitis,CP)和冠心病 (coronary heart disease,CHD)易感性的关系。 方法:采用序列特异引物多聚酶链反应(SSP- PCR)法和多聚酶链反应 -限制性片段长度多态性 (PCR- RFL P)法 ,收集中重度 CP12 8例、CHD10 5例、中重度CP和 CHD10 8例和同族健康对照 130例个体的颊粘膜拭子 ,提取 DNA,测定 IL - 1B- 5 11位点的基因型 ,比较各组间基因型检出率的差别。 结果:IL- 1B- 5 11等位基因 2在 CHD中的检出率显著高于健康组 ,而在中重度 CP与健康组、中重度 CP和 CHD与健康组之间的分布差异无统计学意义。结论:IL - 1B- 5 11等位基因 2可能与 CHD遗传易感性相关。 展开更多
关键词 慢性牙周炎 冠心病 白细胞介素-1b-511 基因型 易感性
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B-1 cells modulate the murine macrophage response to Leishmania major infection 被引量:1
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作者 Angelica F Arcanjo Marise P Nunes +5 位作者 Elias B Silva-Junior Monique Leandro Juliana Dutra Barbosa da Rocha Alexandre Morrot Debora Decote-Ricardo Celio Geraldo Freire-de-Lima 《World Journal of Biological Chemistry》 CAS 2017年第2期151-162,共12页
AIM To investigate the modulatory effect of B-1 cells on murine peritoneal macrophages infected with Leishmania major(L. major) in vitro.METHODS Peritoneal macrophages obtained from BALB/c andBALB/c XID mice were infe... AIM To investigate the modulatory effect of B-1 cells on murine peritoneal macrophages infected with Leishmania major(L. major) in vitro.METHODS Peritoneal macrophages obtained from BALB/c andBALB/c XID mice were infected with L. major and cultured in the presence or absence of B-1 cells obtained from wild-type BALB/c mice. Intracellular amastigotes were counted, and interleukin-10(IL-10) production was quantified in the cellular supernatants using an enzymelinked immunosorbent assay. The levels of the lipid mediator prostaglandin E2(PGE2) were determined using a PGE2 enzyme immunoassay kit(Cayman Chemical, Ann Arbor, MI), and the number of lipid bodies was quantified in the cytoplasm of infected macrophages in the presence and absence of B-1 cells. Culturing the cells with selective PGE2-neutralizing drugs inhibited PGE2 production and confirmed the role of this lipid mediator in IL-10 production. In contrast, we demonstrated that B-1 cells derived from IL-10 KO mice did not favor the intracellular growth of L. major.RESULTS We report that B-1 cells promote the growth of L. major amastigotes inside peritoneal murine macrophages. We demonstrated that the modulatory effect was independent of physical contact between the cells, suggesting that soluble factor(s) were released into the cultures. We demonstrated in our co-culture system that B-1 cells trigger IL-10 production by L. major-infected macrophages. Furthermore, the increased secretion of IL-10 was attributed to the presence of the lipid mediator PGE2 in supernatants of L. major-infected macrophages. The presence of B-1 cells also favors the production of lipid bodies by infected macrophages. In contrast, we failed to obtain the same effect on parasite replication inside L. major-infected macrophages when the B-1 cells were isolated from IL-10 knockout mice. CONCLUSION Our results show that elevated levels of PGE2 and IL-10 produced by B-1 cells increase L. major growth, as indicated by the number of parasites in cell cultures. 展开更多
关键词 Leishmania major MACROPHAGES b-1 cells interleukin-10 Prostaglandin E2 INFECTION
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A comprehensive meta-analysis of the association between three <i>IL</i>1B polymorphisms and rheumatoid arthritis
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作者 Dongjun Dai Lingyan Wang +13 位作者 Limin Xu Lingling Tang Xuting Xu Huadan Ye Xingyu Zhou Cheng Chen Guanghui Pan Ping Ru Qingqing Ma Yi Jiang Wenjing Yu Leiting Xu Meng Ye Shiwei Duan 《Advances in Bioscience and Biotechnology》 2014年第2期108-116,共9页
Rheumatoid arthritis (RA) is an immune-mediated chronic inflammatory disease that causes huge destruction to human body. IL1B encodes key mediator IL-1β protein, which plays an important role in the pathogenesis of i... Rheumatoid arthritis (RA) is an immune-mediated chronic inflammatory disease that causes huge destruction to human body. IL1B encodes key mediator IL-1β protein, which plays an important role in the pathogenesis of inflammatory syndromes. The aim of this study was to evaluate the association between IL1B polymorphisms and RA. A meta-analysis was performed on the association between three IL1B polymorphisms (IL1B-31: rs1143627;IL1B-511: rs16944;IL1B + 3954: rs1143634) and RA. A trend of significant association was observed between IL1B + 3954 and RA (p = 0.06, odd ratio (OR) = 1.19, 95% confidential interval (CI) = 1.00-1.42). A significant association was found in Europeans under the dominant model between IL1B-511T and RA (p = 0.03, OR = 0.89, 95% CI = 0.81-0.99). Our meta-analysis indicated that IL1B ? 511-T played a protective role against RA in Europeans, and that IL1B + 3954-T had the potential to increase the risk of RA. Future large-scale studies should be considered to confirm the association between IL1B polymorphisms and RA. 展开更多
关键词 RHEUMATOID ARTHRITIS META-ANALYSIS Polymorphism IL1b-511 Dominant Model
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白细胞介素-1B-511单核苷酸多态性与胃癌关系的流行病学研究 被引量:1
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作者 曾志荣 陈斌 +3 位作者 胡品津 李初俊 庞瑞萍 陈旻湖 《中华消化杂志》 CAS CSCD 北大核心 2005年第12期710-713,共4页
目的研究中国胃癌高、低发区白细胞介素(IL)-1B-511单核苷酸多态性、幽门螺杆菌(Hp)感染与胃癌的关系。方法胃癌高发区(陕西省)胃癌患者、健康志愿者各102例,胃癌低发区(广东省)胃癌患者、健康志愿者各104例,两组人群在性别比及年龄上... 目的研究中国胃癌高、低发区白细胞介素(IL)-1B-511单核苷酸多态性、幽门螺杆菌(Hp)感染与胃癌的关系。方法胃癌高发区(陕西省)胃癌患者、健康志愿者各102例,胃癌低发区(广东省)胃癌患者、健康志愿者各104例,两组人群在性别比及年龄上均匹配。采用限制性片段长度多态性(PCR-RFLP)分析 IL-1B-511甲核苷酸多态性,酶联免疫吸附法(ELISA)检测血清抗 Hp-IgG 抗体。结果在胃癌低发区,胃癌患者 IL-1B-511 T/T 基因型频率明显高于对照人群(26.9%比13.4%,x^2=5.85,P<0.05;OR=2.37.95% CI 为1.16~4.82)。在胃癌高发区,胃癌患者 IL-1B-511 T/T 基因型频率与对照人群无明显差异(27.5%比24.5%,x^2-0.41,P>0.05);高发区对照人群的 IL-1B-511 T/T基因型频率明显高于低发区相应人群(24.5%比13.4%.x^2=4.1,P<0.05)。Hp 感染轻度增加低发区人群发生胃癌的危险性(OR=3.03,95%CI 为1.61~5.71),而 IL-1B-511 T/T 基因型增加 Hp 感染后胃癌发生的危险性(OR=8.0.95%CI 为1.39~35.7)。结论 IL-1B-511 T/T 基因型与中国人胃癌发生有关,IL-1B-511 T/T 基因型增加 Hp 感染后胃癌发生的危险性。 展开更多
关键词 胃肿瘤 白细胞介素-1b-511 多态性 单核苷酸 幽门螺杆菌
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Single-tube-genotyping of gastric cancer related SNPs by directly using whole blood and paper-dried blood as starting materials 被引量:3
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作者 Huan Huang Ying Bu Guo-Hua Zhou 《World Journal of Gastroenterology》 SCIE CAS CSCD 2006年第24期3814-3820,共7页
AIM: To demonstrate an inexpensive method for typing gastric cancer related single nucleotide polymorphisms (SNPs) using whole blood or paper-dried blood as starting materials. METHODS: PCR amplification is directly c... AIM: To demonstrate an inexpensive method for typing gastric cancer related single nucleotide polymorphisms (SNPs) using whole blood or paper-dried blood as starting materials. METHODS: PCR amplification is directly carried out from the whole blood or paper-dried blood sample without any DNA extraction step. Before PCR, a blood sample, four primers, and all of biological reagents necessary for PCR were added at a time; After PCR, the amplified products were directly separated by slab gel electrophoresis or microchip CE without any purification. SNP typing was performed by tetra-primer PCR with two inner primers specific to each allele and two outer primers defining the length of allele-specific amplicons. Genotypes were directly discriminated by the size of amplicons specific to each allele, thereby avoiding any post-PCR process. RESULTS: Using a special PCR buffer, inhibitory substances in blood (including the anticoagulant in blood) and filter paper were effectively suppressed; a 'true' single-tube-genotyping is thus realized. We successfully determined genotypes IL-1B-511 and IL-1B-31 polymorphisms at the gene IL-1B by using whole-blood and paper-dried blood samples as starting materials respectively. The method is so sensitive that 0.5-1.0μL of blood sample is enough to give a satisfactory typing results. The genotyping results were confirmed by RFLP-PCR using purified genome DNA, indicating that amplification specificity was not affected by inhibitory components (including coagulants) in blood or filter paper. CONCLUSION: Compared with SNP typing methods based on purified DNA, the proposed method is labor saving, simple, inexpensive, and less cross-contaminated. It is promising to use this method to type other SNPs. 展开更多
关键词 Gastric cancer Whole blood Paper-driedblood Tetra-PCR SNP IL-1b-31 IL-1b-511
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(5R)-5-hydroxytriptolide inhibits the inflammatory cascade reaction in astrocytes 被引量:1
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作者 Yan-Qiu Cui Yan Zheng +3 位作者 Gui-Lian Tan Dong-Mei Zhang Jun-Ya Wang Xiao-Min Wang 《Neural Regeneration Research》 SCIE CAS CSCD 2019年第5期913-920,共8页
Many studies have shown that(5R)-5-hydroxytriptolide is the optimal modified analogue of triptolide, possessing comparable immunosuppressive activity but much lower cytotoxicity than triptolide. Whether(5R)-5-hydroxyt... Many studies have shown that(5R)-5-hydroxytriptolide is the optimal modified analogue of triptolide, possessing comparable immunosuppressive activity but much lower cytotoxicity than triptolide. Whether(5R)-5-hydroxytriptolide has preventive effects on neuroinflammation is unclear. This study was designed to pretreat primary astrocytes from the brains of neonatal Sprague-Dawley rats with 20, 100 and 500 nM(5R)-5-hydroxytriptolide for 1 hour before establishing an in vitro neuroinflammation model with 1.0 μg/mL lipopolysaccharide for 24 hours. The generation of nitric oxide was detected by Griess reagents. Astrocyte marker glial fibrillary acidic protein was measured by immunohistochemical staining. The levels of tumor necrosis factor-α and interleukin-1β in the culture supernatant were assayed by enzyme linked immunosorbent assay. Nuclear factor-κB/p65 expression was examined by immunofluorescence staining. The phosphorylation of inhibitor of nuclear factor IκB-α and the location of nuclear factor-κB/P65 were determined using western blot assay. Our data revealed that(5R)-5-hydroxytriptolide inhibited the generation of nitric oxide, tumor necrosis factor-α and interleukin-1β from primary astrocytes activated by lipopolysaccharide, decreased the positive reaction intensity of glial fibrillary acidic protein, reduced the expression of tumor necrosis factor alpha and interleukin-1β in culture supernatant, inhibited the phosphorylation of IκB-α and the translocation of nuclear factor-κB/P65 to the nucleus. These results have confirmed that(5R)-5-hydroxytriptolide inhibits lipopolysaccharide-induced glial inflammatory response and provides cytological experimental data for(5R)-5-hydroxytriptolide in the treatment of neurodegenerative diseases. 展开更多
关键词 NEUROINFLAMMATION (5R)-5-hydroxytriptolide tumor necrosis factor-α interleukin-1Β NITRIC oxide nuclear factor-κB/P65 b-Α microglia neural regeneration
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