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Isolation and Identification of Photobacterium damselae subsp.damselae(PDD) from Tongue Sole 被引量:2
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作者 Yang Nan Zhang Zhiqiang +6 位作者 Wu Tonglei Zhu Jianxin Fu Yanfang Han Hongsheng Wang Hongbin Shi Qiumei Gao Guisheng 《Animal Husbandry and Feed Science》 CAS 2018年第2期99-102,114,共5页
In May 2016,an epizootic occured among cultured tongue soles caused mass deaths in a fish farm in Qinhuangdao,China.In order to find out the etiological agent,a bacterial strain was isolated from ascites and other tis... In May 2016,an epizootic occured among cultured tongue soles caused mass deaths in a fish farm in Qinhuangdao,China.In order to find out the etiological agent,a bacterial strain was isolated from ascites and other tissues of sick tongue sole aseptically collected.The isolate was identified as Photobacterium damselae subsp.damsela(PDD) by isolation culture,Gram staining,physiological identification,morohological observation,biochemical identification and 16S rDNA sequence analysis.The results showed that the isolate shared 99.6% homology with the reference strain in GenBank.The animal regression test displayed that the isolate had very strong pathogenicity to tongue sole.The LD(50) was 3.1 × 10~4 CFU/mL,and it showed pathogenicity to mammals.The antimicrobial susceptibility test showed the isolate was highly sensitive to nrofloxacin,Norfloxacin,Ciprofloxacin,Mequindox;moderately sensitive to Cefradine,Doxycycline;and insensitive to Gentamicin,Ceftriaxone,Tilmicosin,etc.. 展开更多
关键词 Tongue sole Pholobacterium damselae subsp damselae (PDI) isolation and identification: Antimicrobial susceptibility test
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Separation and Preliminary Identification of Intestinal Probiotics of Laying Hens
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作者 Guo Shijin Zhang Zhimei +4 位作者 Fu Shijun Mo Ling Zhang Ying Shen Zhiqiang Huang Baohua 《Animal Husbandry and Feed Science》 CAS 2018年第1期72-74,共3页
[Objective] The paper was to isolate and identify probiotics in the intestine of laying hens. [Method] The intestinal probiotics in laying hens at peak period were isolated using conventional separation methods; the p... [Objective] The paper was to isolate and identify probiotics in the intestine of laying hens. [Method] The intestinal probiotics in laying hens at peak period were isolated using conventional separation methods; the physical and chemical properties of target strains and in vitro antibacterial effects were measured. Moreover, the safety test of chicks was conducted. [Result] Four strains of lactobacillus were isolated from the jejunum of laying hens, including Lactobacillus plantarum, Bacillus acidophilus, L. delbrueckii subsp. Delbrueckii and L. delbrueckii subsp. Lactis, and their inhibition zone diameters were 18.30, 16.07, 11.45, 17.26 mm, respectively. One strain of Lactobacillus, L. brevis, was isolated from the cecum, with the inhibition zone diameter of 10.26 mm. Three strains of bacillus were isolated from the cecum, including Bacillus subtilis, B. cereus and B. licheniformis, and their inhibition zone diameters were 9.25, 8.46 and 8.37 mm, respectively. Daily drinking 2 billion units of viable bacteria was the safe dosage for chicks. [Conclusion] Eight strains of probiotics had certain inhibitory effect on Escherichia coli O;, and had no toxic side effects to chicks. Lactobacillus had strong antibacterial effect on E. coli O;, while the antibacterial effect of bacillus was relatively weak. 展开更多
关键词 Laying hen Intestine Probiotics isolation Identification Physical and chemical properties Antibacterial Safety test
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明显的缺失:美育在美国 被引量:1
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作者 朱璟(译) 《美育学刊》 2020年第6期12-19,共8页
美育立足于艺术正趋于消亡的一片土地上,这是因为,尽管《有教无类法案》(No Child Left Behind Act)将艺术命名为核心科目,《让每个学生成功法案》(Every Student Succeeds Act)认为其对高质量的教育至关重要,但艺术项目仍被降至最低地... 美育立足于艺术正趋于消亡的一片土地上,这是因为,尽管《有教无类法案》(No Child Left Behind Act)将艺术命名为核心科目,《让每个学生成功法案》(Every Student Succeeds Act)认为其对高质量的教育至关重要,但艺术项目仍被降至最低地位。由于艺术既不是考试内容,也未得到资助,于是在一个由市场经济驱动,由数学和语言艺术其次是科学与经济学推动的世界里,艺术变得脆弱不堪。越来越多的研究机构指出艺术对学生的学业、高校成就和公民参与度存在影响,在此背景下所要探讨的问题是:我们如何走到了艺术缺位的危险地步?最重要的是,在正式的教育体系中、在工作中、在现在和将来的生活中,艺术对学生有何价值? 展开更多
关键词 美育 艺术 州立考试 孤立主义 工具主义 社会公正观点
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