Aim: This dissection study was conducted to verify if the Myofascial kinetic lines, outlined in detail in humans and recently documented in horses, were present in dogs. These dynamic lines present rows of interconnec...Aim: This dissection study was conducted to verify if the Myofascial kinetic lines, outlined in detail in humans and recently documented in horses, were present in dogs. These dynamic lines present rows of interconnected muscles, myofascia and other fascia structures, which influence the biomechanics of the spine and limbs. Methods: Forty-two dogs of different breeds and genders were dissected, imaged, and videoed. Results: Similar kinetic lines were verified in the dog, as described in humans and horses, and additionally, three new branches of the lines were discovered. The kinetic lines described were three superficial lines: Dorsal, Ventral, and Lateral, which all started in the hindlimb and ended in the temporal and occipital regions. These lines act respectively in spinal extension, flexion, and lateral flexion. Three profound lines, which started in the tail and ended in the head. The Deep Dorsal Line followed the transversospinal myofascia. The Deep Ventral Line showed an additional start deep in the medial hind limb, continued in the hypaxial myofascia, and enveloped all the viscera. Also, the Deep Lateral Line started in the hindlimb but parted along the trunk in the deep lateral myofascial structures. Two helical lines crossed the midline two or three times and served to rotate the spine. The Functional Line established a sling from the axilla to the contralateral stifle and presented a new ipsilateral branch. The Spiral Line connected the head and the ipsilateral tarsus and additionally presented a new straight branch. The four front limb lines describe their motion: the Front Limb Protraction and Retraction, Adduction, and Abduction Lines. Conclusion: The canine lines mirrored the equine and human lines with exceptions due to differences in anatomy, foot posture, lumbosacral flexibility, and their biomechanical constitution as predator versus prey animals. Additionally, three new canine branches were verified and described.展开更多
Objective:To characterize the infection patterns and growth characteristics of the Zika virus(ZIKV)strain JMB-185 from Indonesia in various mammalian cell lines.Methods:ZIKV was grown in human(A549,HEK293,HepG2,Huh7,J...Objective:To characterize the infection patterns and growth characteristics of the Zika virus(ZIKV)strain JMB-185 from Indonesia in various mammalian cell lines.Methods:ZIKV was grown in human(A549,HEK293,HepG2,Huh7,Jurkat,and THP-1)and non-human mammalian(RAW264.7,Vero,and Vero76)cell lines.Viral replication kinetics were measured using plaque assay,while intra-and extracellular viral RNA concentrations were assessed using RT-PCR.Flow cytometry was used to quantify the infected cells and cell viability was measured using an MTT assay.The ability of ZIKV to infect cell lines was visualized using a fluorescence immunostaining assay.Results:This ZIKV strain preferentially infected the lung,kidney,and liver cell lines A549,HEK293,Huh7,Vero,and Vero76,but not the immune cells Jurkat,RAW264.7,and THP-1.By contrast,the ZIKV showed no sign of infection in HepG2 cells,while maintaining viral titer over 3 days post-infection,with no infection recorded in immunostaining,no increase in viral RNA,and no indication of cell deterioration.Conclusions:The Indonesian ZIKV strain has a similar infection profile as other strains,except for its poor infectivity on HepG2 cells.Information on the growth characteristics of Indonesia ZIKV will help expand our understanding of the biology of ZIKV which will be useful for various applications including antiviral discovery.展开更多
We investigate the relationship between the variability of broad absorption lines(BALs)or narrow absorption lines(NALs)and that of continuum using a data set of two-epoch SDSS spectra containing 134 C IV NAL-BAL pairs...We investigate the relationship between the variability of broad absorption lines(BALs)or narrow absorption lines(NALs)and that of continuum using a data set of two-epoch SDSS spectra containing 134 C IV NAL-BAL pairs.Our analysis reveals an anti-correlation between the fractional equivalent width(EW)variations in NALs(or BALs)and the fractional flux variations of the continuum,with Spearman rank correlation coefficients of r=-0.47(p=1E-08)and r=-0.58(p=1E-13),respectively.In addition,we find a positive correlation between the fractional EW variations in NALs and BALs(r=0.72,p=1E-22),and derive a regression equation ΔEW_(NAL)/<EW_(NAL)>=0.803ΔEW_(BAL)/<EW_(BAL)>+0.008,with an intrinsic scatter of 0.14.These results suggest that the variability in the ionizing continuum may play a significant role in the observed changes in C IV NALs and BALs,supporting the idea of photoionization-driven variability.The co-variability between C IV NALs and BALs may imply that they originate from outflows with similar physical conditions.展开更多
BACKGROUND Thrombocytopenia 2,an autosomal dominant inherited disease characterized by moderate thrombocytopenia,predisposition to myeloid malignancies and normal platelet size and function,can be caused by 5’-untran...BACKGROUND Thrombocytopenia 2,an autosomal dominant inherited disease characterized by moderate thrombocytopenia,predisposition to myeloid malignancies and normal platelet size and function,can be caused by 5’-untranslated region(UTR)point mutations in ankyrin repeat domain containing 26(ANKRD26).Runt related transcription factor 1(RUNX1)and friend leukemia integration 1(FLI1)have been identified as negative regulators of ANKRD26.However,the positive regulators of ANKRD26 are still unknown.AIM To prove the positive regulatory effect of GATA binding protein 2(GATA2)on ANKRD26 transcription.METHODS Human induced pluripotent stem cells derived from bone marrow(hiPSC-BM)INTRODUCTION Ankyrin repeat domain containing protein 26(ANKRD26)acts as a regulator of adipogenesis and is involved in the regulation of feeding behavior[1-3].The ANKRD26 gene is located on chromosome 10 and shares regions of homology with the primate-specific gene family POTE.According to the Human Protein Atlas database,the ANKRD26 protein is localized to the Golgi apparatus and vesicles,and its expression can be detected in nearly all human tissues[4].Moreover,UniProt annotation revealed that ANKRD26 is localized in the centrosome and contains coiled-coil domains formed by spectrin helices and ankyrin repeats[5,6].The most common disease related to ANKRD26 is thrombocytopenia 2(THC2),which is a rare autosomal dominant inherited disease characterized by lifelong mild-to-moderate thrombocytopenia and mild bleeding[7-9].Caused by the variants in the 5’-untranslated region(UTR)of ANKRD26,THC2 is defined by a decrease in the number of platelets in circulating blood and results in increased bleeding and decreased clotting ability[8,10].Due to the point mutations that occur in the 5’-UTR of ANKRD26,its negative transcription factors(TFs),Runt related transcription factor 1(RUNX1)and friend leukemia integration 1(FLI1),lose their repression effect[11].The persistent expression of ANKRD26 increases the activity of the mitogen activated protein kinase and extracellular signal regulated kinase 1/2 signaling pathways,which are potentially involved in the regulation of thrombopoietin-dependent signaling and further impair proplatelet formation by megakaryocytes(MKs)[11].However,the positive regulators of ANKRD26,which might be associated with THC2 pathology,are still unknown.展开更多
The development of spectroscopic survey telescopes like Large Sky Area Multi-Object Fiber Spectroscopic Telescope(LAMOST),Apache Point Observatory Galactic Evolution Experiment and Sloan Digital Sky Survey has opened ...The development of spectroscopic survey telescopes like Large Sky Area Multi-Object Fiber Spectroscopic Telescope(LAMOST),Apache Point Observatory Galactic Evolution Experiment and Sloan Digital Sky Survey has opened up unprecedented opportunities for stellar classification.Specific types of stars,such as early-type emission-line stars and those with stellar winds,can be distinguished by the profiles of their spectral lines.In this paper,we introduce a method based on derivative spectroscopy(DS)designed to detect signals within complex backgrounds and provide a preliminary estimation of curve profiles.This method exhibits a unique advantage in identifying weak signals and unusual spectral line profiles when compared to other popular line detection methods.We validated our approach using synthesis spectra,demonstrating that DS can detect emission signals three times fainter than Gaussian fitting.Furthermore,we applied our method to 579,680 co-added spectra from LAMOST Medium-Resolution Spectroscopic Survey,identifying 16,629 spectra with emission peaks around the Hαline from 10,963 stars.These spectra were classified into three distinct morphological groups,resulting in nine subclasses as follows.(1)Emission peak above the pseudo-continuum line(single peak,double peaks,emission peak situated within an absorption line,P Cygni profile,Inverse P Cygni profile);(2)Emission peak below the pseudo-continuum line(sharp emission peak,double absorption peaks,emission peak shifted to one side of the absorption line);(3)Emission peak between the pseudo-continuum line.展开更多
The synthesis of new 4-imino-4H-chromeno[2,3-d]pyrimidin-3(5H)-amine in four steps including one step under microwave dielectric heating is reported. The structural identity of the synthesized compounds was establishe...The synthesis of new 4-imino-4H-chromeno[2,3-d]pyrimidin-3(5H)-amine in four steps including one step under microwave dielectric heating is reported. The structural identity of the synthesized compounds was established according to their spectroscopic analysis, such as FT-IR, NMR and mass spectroscopy. These new compounds were tested for their antiproliferative activities on seven representative human tumoral cell lines (Huh7 D12, Caco2, MDA-MB231, MDA-MB468, HCT116, PC3 and MCF7) and also on fibroblasts. Among them, only the compounds 6c showed micromolar cytotoxic activity on tumor cell lines (1.8 50 50 > 25 μM). Finally, in silico ADMET studies ware performed to investigate the possibility of using of the identified compound 6c as potential anti-tumor compound.展开更多
This article is based on a recent model specifically defining magnetic field values around electrical wires. With this model, calculations of field around parallel wires were obtained. Now, this model is extended with...This article is based on a recent model specifically defining magnetic field values around electrical wires. With this model, calculations of field around parallel wires were obtained. Now, this model is extended with the new concept of magnetic equipotential surface to magnetic field curves around crossing wires. Cases of single, double, and triple wires are described. Subsequent article will be conducted for more general scenarios where wires are neither infinite nor parallel.展开更多
In recent years,with the introduction of the strategy to strengthen the country’s transportation infrastructure and the continuous implementation of new economic development models such as integrating transportation ...In recent years,with the introduction of the strategy to strengthen the country’s transportation infrastructure and the continuous implementation of new economic development models such as integrating transportation with tourism,various open service areas integrating highway service areas with local tourism have emerged nationwide.Examples include the Yangcheng Lake service area in Jiangsu and the Lengshui service area in Chongqing.This paper focuses on the design example of the Dazu Stone Carving service area on the Chongqing section of the Yurong Expressway,comprehensively considering factors such as construction scale,terrain conditions,and local urban planning.It analyzes and studies the traffic flow design of unilateral agglomerative open service areas around two vehicle traffic conversion links:between the main expressway and the service area,and between the service area and local roads.The aim is to provide a case study reference for similar projects.展开更多
Rapeseed(Brassica napus,AACC)was formed by hybridization between progenitor species Brassica rapa(AA)and Brassica oleracea(CC).As a result of a limited number of hybridization events between specific progenitor genoty...Rapeseed(Brassica napus,AACC)was formed by hybridization between progenitor species Brassica rapa(AA)and Brassica oleracea(CC).As a result of a limited number of hybridization events between specific progenitor genotypes and strong breeding selection for oil quality traits,rapeseed has limited genetic diversity.The production of resynthesized B.napus lines via interspecific hybridization of the diploid progenitor species B.rapa and B.oleracea is one possible way to increase genetic variation in rapeseed.However,most resynthesized lines produced so far have been reported to be meiotically unstable and infertile,in contrast to established B.napus cultivars.This hinders both maintenance and use of this germplasm in breeding programs.We characterized a large set of 140 resynthesized lines produced by crosses between B.rapa and B.oleracea,as well as between B.rapa and wild C genome species(B.incana,B.hilarionis,B.montana,B.Bourgeaui,B.villosa and B.cretica)for purity(homozygosity),fertility,and genome stability.Self-pollinated seed set,seeds per ten pods as well as percentage pollen viability were used to estimate fertility.SNP genotyping was performed using the Illumina Infinium Brassica 60K array for 116 genotypes,with at least three individuals per line.Most of the material which had been advanced through multiple generations was no longer pure,with heterozygosity detected corresponding to unknown parental contributions via outcrossing.Fertility and genome stability were both genotypedependent.Most lines had high numbers of copy number variants(CNVs),indicative of meiotic instability,and high numbers of CNVs were significantly associated with reduced fertility.Eight putatively stable resynthesized B.napus lines were observed.Further investigation of these lines may reveal the mechanisms underlying this effect.Our results suggest that selection of stable resynthesized lines for breeding purposes is possible.展开更多
Micrometric-thin cells(MCs)with alkali vapor atoms have been valuable for research and applications of hyperfine Zeeman splitting and atomic magnetometers under strong magnetic fields.We theoretically and experimental...Micrometric-thin cells(MCs)with alkali vapor atoms have been valuable for research and applications of hyperfine Zeeman splitting and atomic magnetometers under strong magnetic fields.We theoretically and experimentally study the saturated absorption spectra using a 100-μm cesium MC,where the pump and probe beams are linearly polarized with mutually perpendicular polarizations,and the magnetic field is along the pump beam.Because of the distinctive thin chamber of the MC,crossover spectral lines in saturated absorption spectra are largely suppressed leading to clear splittings of hyperfine Zeeman transitions in experiments,and the effect of spatial magnetic field gradient is expected to be reduced.A calculation method is proposed to achieve good agreements between theoretical calculations and experimental results.This method successfully explains the suppression of crossover lines in MCs,as well as the effects of magnetic field direction,propagation and polarization directions of the pump/probe beam on saturated absorption spectrum.The saturated absorption spectrum with suppressed crossover lines is used for laser frequency stabilization,which may provide the potential value of MCs for high spatial resolution strong-field magnetometry with high sensitivity.展开更多
The YOLOx-s network does not sufficiently meet the accuracy demand of equipment detection in the autonomous inspection of distribution lines by Unmanned Aerial Vehicle(UAV)due to the complex background of distribution...The YOLOx-s network does not sufficiently meet the accuracy demand of equipment detection in the autonomous inspection of distribution lines by Unmanned Aerial Vehicle(UAV)due to the complex background of distribution lines,variable morphology of equipment,and large differences in equipment sizes.Therefore,aiming at the difficult detection of power equipment in UAV inspection images,we propose a multi-equipment detection method for inspection of distribution lines based on the YOLOx-s.Based on the YOLOx-s network,we make the following improvements:1)The Receptive Field Block(RFB)module is added after the shallow feature layer of the backbone network to expand the receptive field of the network.2)The Coordinate Attention(CA)module is added to obtain the spatial direction information of the targets and improve the accuracy of target localization.3)After the first fusion of features in the Path Aggregation Network(PANet),the Adaptively Spatial Feature Fusion(ASFF)module is added to achieve efficient re-fusion of multi-scale deep and shallow feature maps by assigning adaptive weight parameters to features at different scales.4)The loss function Binary Cross Entropy(BCE)Loss in YOLOx-s is replaced by Focal Loss to alleviate the difficulty of network convergence caused by the imbalance between positive and negative samples of small-sized targets.The experiments take a private dataset consisting of four types of power equipment:Transformers,Isolators,Drop Fuses,and Lightning Arrestors.On average,the mean Average Precision(mAP)of the proposed method can reach 93.64%,an increase of 3.27%.The experimental results show that the proposed method can better identify multiple types of power equipment of different scales at the same time,which helps to improve the intelligence of UAV autonomous inspection in distribution lines.展开更多
The classical minimization of power losses in transmission lines is dominated by artificial intelligence techniques, which do not guarantee global optimum amidst local minima. Revolutionary and evolutionary techniques...The classical minimization of power losses in transmission lines is dominated by artificial intelligence techniques, which do not guarantee global optimum amidst local minima. Revolutionary and evolutionary techniques are encumbered with sophisticated transformations, which weaken the techniques. Power loss minimization is crucial to the efficient design and operation of power transmission lines. Minimization of losses is one way to meet steady grid supply, especially at peak demand. Thus, this paper has presented a gradient technique to obtain optimal variables and values from the power loss model, which efficiently minimizes power losses by modifying the traditional power loss model that combines Ohm and Corona losses. Optimality tests showed that the unmodified model does not support the minimization of power losses on transmission lines as the Hessian matrix portrayed the maximization of power losses. However, the modified model is consistent with the gradient method of optimization, which yielded optimum variables and values from the power loss model developed in this study. The unmodified (modified) models for Bujagali-Kawanda 220 kV and Masaka West-Mbarara North 132 kV transmission lines in Uganda showed maximum power losses of 0.406 (0.391) and 0.452 (0.446) kW/km/phase respectively. These results indicate that the modified model is superior to the unmodified model in minimizing power losses in the transmission lines and should be implemented for the efficient design and operation of power transmission lines within and outside Uganda for the same transmission voltages.展开更多
In this paper, we have studied several classes of planar piecewise Hamiltonian systems with three zones separated by two parallel straight lines. Firstly, we give the maximal number of limit cycles in these classes of...In this paper, we have studied several classes of planar piecewise Hamiltonian systems with three zones separated by two parallel straight lines. Firstly, we give the maximal number of limit cycles in these classes of systems with a center in two zones and without equilibrium points in the other zone (or with a center in one zone and without equilibrium points in the other zones). In addition, we also give examples to illustrate that it can reach the maximal number.展开更多
A miniaturized broadband Wilkinson power divider is proposed. Micro-strip branch lines are introduced to replace multiple resistors used in multi-stage Wilkinson power dividers to increase the bandwidth of single-stag...A miniaturized broadband Wilkinson power divider is proposed. Micro-strip branch lines are introduced to replace multiple resistors used in multi-stage Wilkinson power dividers to increase the bandwidth of single-stage Wilkinson power dividers. To demonstrate its performance, an improved single-stage Wilkinson power divider with four micro-strip branch lines was designed. Simulated results show that the insert loss is better than 3.2 dB, the input return loss, output return loss, and isolation are better than 15 dB respectively, across a 76% bandwidth from 18 to 40 GHz. .展开更多
Rye(Secale cereale)is a valuable gene donor for wheat improvement,especially for its resistance to diseases.Developing rye-derived resistance sources is important for wheat breeding.In the present study,two wheat-rye ...Rye(Secale cereale)is a valuable gene donor for wheat improvement,especially for its resistance to diseases.Developing rye-derived resistance sources is important for wheat breeding.In the present study,two wheat-rye derivatives,designated JS016 and JS110,were produced by crossing common wheat cultivar Yangmai 23 with Pakistani rye accession W2A.Using sequential genomic in situ hybridization(GISH)and multicolor fluorescence in situ hybridization(mc-FISH),JS016 and JS110 were identified as a T6BS.6RL translocation line and a T6BS.6BL6RL translocation line,respectively.Ten newly 6RL chromosome arm-specific markers were developed and used to confirm the 6RL translocation.The wheat 55K single-nucleotide polymorphism(SNP)array further verified the molecular cytogenetic identification results above and clarified their breakpoints at 430.9 and 523.0 Mb of chromosome 6B in JS016 and JS110,respectively.Resistance spectrum and allelism test demonstrated that JS016 and JS110 possessed novel powdery mildew resistance gene(s)that was derived from the 6RL translocation but differed from Pm20.Moreover,JS016 and JS110 had better agronomic traits than the previously reported 6RL translocation line carrying Pm20.To efficiently transfer and detect the 6RL translocation from JS016 and JS110,one 6RL-specific Kompetitive allele specific PCR(KASP)marker was developed and validated in high throughput marker-assisted selection(MAS).展开更多
Peanut(Arachis hypogaea L.)is an oil and economic crop of vital importance,and peanut pod is the key organ influencing the yield and processing quality.Hence,the Pod-related traits(PRTs)are considered as important agr...Peanut(Arachis hypogaea L.)is an oil and economic crop of vital importance,and peanut pod is the key organ influencing the yield and processing quality.Hence,the Pod-related traits(PRTs)are considered as important agronomic traits in peanut breeding.To broaden the variability of PRTs in current peanut germplasms,three elite peanut cultivars were used to construct Ethyl methane sulfonate(EMS)-induced mutant libraries in this study.The optimal EMS treatment conditions for the three peanut varieties were determined.It was found that the median lethal dose(LD50)of EMS treatment varied greatly among different genotypes.Finally,the EMS-induced peanut mutant libraries were constructed and a total of 124 mutant lines for PRTs were identified and evaluated.Furthermore,“M-8070”,one of the mutant lines for pod constriction,was re-sequenced via high-throughput sequencing technology.The genome-wide variations between“M-8070”and its wild parent“Fuhua 8”(FH 8)were detected.2994 EMS-induced single nucleotide polymorphisms(SNPs)and 1188 insertion-deletions(InDels)between“M-8070”and its wild parent were identified.The predominant SNP mutation type was C/G to T/A transitions,while the predominant InDel mutation type was“1-bp”.We analyzed the distribution of identified mutations and annotated their functions.Most of the mutations(91.68%of the SNPs and 77.69%of the InDels)were located in the intergenic region.72 SNPs were identified in the exonic region,leading to 27 synonymous,43 nonsynonymous and 2 stop-gain variation for gene structure.13 Indels were identified in the exonic region,leading to 4 frame-shift,8 non-frame-shift and 1 stop-gain variations of genes.These mutations may lead to the phenotypic variation of“M-8070”.Our study provided valuable resources for peanut improvement and functional genomic research.展开更多
Eight indica ( Oryza sativa L.) environment-sensitive genic male-sterile (EGMS) lines, 2-2S, K1405S, F131S, 2136S, Pei-Ai 64S, 1290S, GD-IS and N17S, were sequentially seeded with 10-15 d interval at three sites, Wuha...Eight indica ( Oryza sativa L.) environment-sensitive genic male-sterile (EGMS) lines, 2-2S, K1405S, F131S, 2136S, Pei-Ai 64S, 1290S, GD-IS and N17S, were sequentially seeded with 10-15 d interval at three sites, Wuhan in 1997, Guiyang in 1997 and Sanya in 1997 and 1998, China. The results of investigations on self-sterilities showed that all of eight EGMS lines had stable sterile periods of longer than 30 d at Wuhan. They can be used for seed production of two-line hybrid rice, but can not reproduce themselves. Their stable sterile periods were shorter than 30 d at Guiyang, they can reproduce themselves and can not be used for hybrid seed production. In Sanya, their stable sterile periods were longer than 150 d, all of eight lines can be used for seed production in summer and autumn and reproduce themselves in winter. The fertility of all eight lines were sensitive to temperature. The sensitive stages, sensitive duration and critical point of temperatures (CPT) of fertility alteration in various lines were different. The sensitive stages of 2-2S and K1405S were from 18 d to 9 d before heading, the sensitive durations were 7-10 d and the CPTs were 23.7-24.5 degreesC. The sensitive stage, sensitive duration and CPT of F131S were from 17 to 5 d before heading, 13 d and 24.3-24.7 degreesC, respectively. The sensitive stage, sensitive duration and CPT of 2136S were from 18 to 12 d before heading, 7 d and 24.6-25.1 degreesC:, respectively. The sensitive stages, sensitive durations of Pei-Ai 64S, 1290S, N17S and GD-1S were from 24 to 13 d before heading and 10-13 d. And their CPTs were 24.6-25.1 degreesC, 25.5-26.2 degreesC, 25.4-26.1 degreesC,, and 24.1-24.7 degreesC, respectively.展开更多
Digital twin(DT) is a virtual replica of a physical world that has become one of the most important ideas in the manufacturing industry’s digital revolution. DT modeling is a vital issue in building a DT of a product...Digital twin(DT) is a virtual replica of a physical world that has become one of the most important ideas in the manufacturing industry’s digital revolution. DT modeling is a vital issue in building a DT of a production line. In this paper, a method is proposed to address the difficulties of complicated production line business and data heterogeneity. The method focuses on essential data in the production line and creates conceptual and information models based on the ArtiFlow model and AutomationML(AML). Conceptual models are mainly used to describe and analyze the business activities of the production line, and information models describe real production lines in the form of XML files. The proposed modeling approach has been applied to a real-world clothing production line to demonstrate its feasibility and effectiveness.展开更多
A total of 28 accessions of rice (Oryza sativa L.) germplasms were collected from International Rice Research Institute (IRRI), Philippine and Gene Pool of China National Rice Research Institute (CNRRI). These germpla...A total of 28 accessions of rice (Oryza sativa L.) germplasms were collected from International Rice Research Institute (IRRI), Philippine and Gene Pool of China National Rice Research Institute (CNRRI). These germplasms were attributed as a Donor Gene Pool, consisting of 27 diverse morphological marker lines with each having a unique valuable trait (gene), and the recurrent parent-Zhefu 802, an early-season indica cultivar in south of China in the 1980s and 1990s. A set of 27 isogenic lines with 2-3 markers on every chromosome were obtained after more than 10 successive backcrosses. Moreover, 15 accessions of double-marker isogenic lines were developed by further pyramiding markers on the same chromosome. Key agronomic traits such as heading date, plant height, tillering number and panicle length in this set of lines were similar to that of Zhefu 802 except the marker traits.展开更多
文摘Aim: This dissection study was conducted to verify if the Myofascial kinetic lines, outlined in detail in humans and recently documented in horses, were present in dogs. These dynamic lines present rows of interconnected muscles, myofascia and other fascia structures, which influence the biomechanics of the spine and limbs. Methods: Forty-two dogs of different breeds and genders were dissected, imaged, and videoed. Results: Similar kinetic lines were verified in the dog, as described in humans and horses, and additionally, three new branches of the lines were discovered. The kinetic lines described were three superficial lines: Dorsal, Ventral, and Lateral, which all started in the hindlimb and ended in the temporal and occipital regions. These lines act respectively in spinal extension, flexion, and lateral flexion. Three profound lines, which started in the tail and ended in the head. The Deep Dorsal Line followed the transversospinal myofascia. The Deep Ventral Line showed an additional start deep in the medial hind limb, continued in the hypaxial myofascia, and enveloped all the viscera. Also, the Deep Lateral Line started in the hindlimb but parted along the trunk in the deep lateral myofascial structures. Two helical lines crossed the midline two or three times and served to rotate the spine. The Functional Line established a sling from the axilla to the contralateral stifle and presented a new ipsilateral branch. The Spiral Line connected the head and the ipsilateral tarsus and additionally presented a new straight branch. The four front limb lines describe their motion: the Front Limb Protraction and Retraction, Adduction, and Abduction Lines. Conclusion: The canine lines mirrored the equine and human lines with exceptions due to differences in anatomy, foot posture, lumbosacral flexibility, and their biomechanical constitution as predator versus prey animals. Additionally, three new canine branches were verified and described.
基金supported by a research grant from the Ministry of Education,Culture,Research and Technology(KEMENDIKBUD RISTEK)number NKB-022/UN2.RST/HKP.05.00/2021 awarded to AB.
文摘Objective:To characterize the infection patterns and growth characteristics of the Zika virus(ZIKV)strain JMB-185 from Indonesia in various mammalian cell lines.Methods:ZIKV was grown in human(A549,HEK293,HepG2,Huh7,Jurkat,and THP-1)and non-human mammalian(RAW264.7,Vero,and Vero76)cell lines.Viral replication kinetics were measured using plaque assay,while intra-and extracellular viral RNA concentrations were assessed using RT-PCR.Flow cytometry was used to quantify the infected cells and cell viability was measured using an MTT assay.The ability of ZIKV to infect cell lines was visualized using a fluorescence immunostaining assay.Results:This ZIKV strain preferentially infected the lung,kidney,and liver cell lines A549,HEK293,Huh7,Vero,and Vero76,but not the immune cells Jurkat,RAW264.7,and THP-1.By contrast,the ZIKV showed no sign of infection in HepG2 cells,while maintaining viral titer over 3 days post-infection,with no infection recorded in immunostaining,no increase in viral RNA,and no indication of cell deterioration.Conclusions:The Indonesian ZIKV strain has a similar infection profile as other strains,except for its poor infectivity on HepG2 cells.Information on the growth characteristics of Indonesia ZIKV will help expand our understanding of the biology of ZIKV which will be useful for various applications including antiviral discovery.
基金supported by the Guangxi Natural Science Foundation(No.2021GXNSFBA220044)the National Natural Science Foundation of China(No.11903002)the Research Project of Baise University(No.2019KN04)。
文摘We investigate the relationship between the variability of broad absorption lines(BALs)or narrow absorption lines(NALs)and that of continuum using a data set of two-epoch SDSS spectra containing 134 C IV NAL-BAL pairs.Our analysis reveals an anti-correlation between the fractional equivalent width(EW)variations in NALs(or BALs)and the fractional flux variations of the continuum,with Spearman rank correlation coefficients of r=-0.47(p=1E-08)and r=-0.58(p=1E-13),respectively.In addition,we find a positive correlation between the fractional EW variations in NALs and BALs(r=0.72,p=1E-22),and derive a regression equation ΔEW_(NAL)/<EW_(NAL)>=0.803ΔEW_(BAL)/<EW_(BAL)>+0.008,with an intrinsic scatter of 0.14.These results suggest that the variability in the ionizing continuum may play a significant role in the observed changes in C IV NALs and BALs,supporting the idea of photoionization-driven variability.The co-variability between C IV NALs and BALs may imply that they originate from outflows with similar physical conditions.
基金Supported by General Program of National Natural Science Foundation of China,No.81770197Scientific and Technological Research Major Program of Chongqing Municipal Education Commission,No.KJZD-M202312802+1 种基金Chongqing Natural Science Foundation of China,No.CSTB2022NSCQ-MSX0190,No.CSTB2022NSCQ-MSX0176,and No.cstc2020jcyj-msxmX0051Xinqiao Young Postdoc Talent Incubation Program,No.2022YQB098.
文摘BACKGROUND Thrombocytopenia 2,an autosomal dominant inherited disease characterized by moderate thrombocytopenia,predisposition to myeloid malignancies and normal platelet size and function,can be caused by 5’-untranslated region(UTR)point mutations in ankyrin repeat domain containing 26(ANKRD26).Runt related transcription factor 1(RUNX1)and friend leukemia integration 1(FLI1)have been identified as negative regulators of ANKRD26.However,the positive regulators of ANKRD26 are still unknown.AIM To prove the positive regulatory effect of GATA binding protein 2(GATA2)on ANKRD26 transcription.METHODS Human induced pluripotent stem cells derived from bone marrow(hiPSC-BM)INTRODUCTION Ankyrin repeat domain containing protein 26(ANKRD26)acts as a regulator of adipogenesis and is involved in the regulation of feeding behavior[1-3].The ANKRD26 gene is located on chromosome 10 and shares regions of homology with the primate-specific gene family POTE.According to the Human Protein Atlas database,the ANKRD26 protein is localized to the Golgi apparatus and vesicles,and its expression can be detected in nearly all human tissues[4].Moreover,UniProt annotation revealed that ANKRD26 is localized in the centrosome and contains coiled-coil domains formed by spectrin helices and ankyrin repeats[5,6].The most common disease related to ANKRD26 is thrombocytopenia 2(THC2),which is a rare autosomal dominant inherited disease characterized by lifelong mild-to-moderate thrombocytopenia and mild bleeding[7-9].Caused by the variants in the 5’-untranslated region(UTR)of ANKRD26,THC2 is defined by a decrease in the number of platelets in circulating blood and results in increased bleeding and decreased clotting ability[8,10].Due to the point mutations that occur in the 5’-UTR of ANKRD26,its negative transcription factors(TFs),Runt related transcription factor 1(RUNX1)and friend leukemia integration 1(FLI1),lose their repression effect[11].The persistent expression of ANKRD26 increases the activity of the mitogen activated protein kinase and extracellular signal regulated kinase 1/2 signaling pathways,which are potentially involved in the regulation of thrombopoietin-dependent signaling and further impair proplatelet formation by megakaryocytes(MKs)[11].However,the positive regulators of ANKRD26,which might be associated with THC2 pathology,are still unknown.
基金the support provided by the National Natural Science Foundation of China(NSFC,Grant Nos.12090040/3,12125303,12288102,and 11733008)the National Key Research and Development Program of China(grant No.2021YFA1600401/3)+3 种基金the China Manned Space Project(CMSCSST-2021-A10)the Yunnan Fundamental Research Projects(grant No.202101AV070001)the National Natural Science Foundation of China and the Chinese Academy of Sciences,under grant No.U1831125the Research Program of Frontier Sciences,CAS(grant No.QYZDY-SSW-SLH007)。
文摘The development of spectroscopic survey telescopes like Large Sky Area Multi-Object Fiber Spectroscopic Telescope(LAMOST),Apache Point Observatory Galactic Evolution Experiment and Sloan Digital Sky Survey has opened up unprecedented opportunities for stellar classification.Specific types of stars,such as early-type emission-line stars and those with stellar winds,can be distinguished by the profiles of their spectral lines.In this paper,we introduce a method based on derivative spectroscopy(DS)designed to detect signals within complex backgrounds and provide a preliminary estimation of curve profiles.This method exhibits a unique advantage in identifying weak signals and unusual spectral line profiles when compared to other popular line detection methods.We validated our approach using synthesis spectra,demonstrating that DS can detect emission signals three times fainter than Gaussian fitting.Furthermore,we applied our method to 579,680 co-added spectra from LAMOST Medium-Resolution Spectroscopic Survey,identifying 16,629 spectra with emission peaks around the Hαline from 10,963 stars.These spectra were classified into three distinct morphological groups,resulting in nine subclasses as follows.(1)Emission peak above the pseudo-continuum line(single peak,double peaks,emission peak situated within an absorption line,P Cygni profile,Inverse P Cygni profile);(2)Emission peak below the pseudo-continuum line(sharp emission peak,double absorption peaks,emission peak shifted to one side of the absorption line);(3)Emission peak between the pseudo-continuum line.
文摘The synthesis of new 4-imino-4H-chromeno[2,3-d]pyrimidin-3(5H)-amine in four steps including one step under microwave dielectric heating is reported. The structural identity of the synthesized compounds was established according to their spectroscopic analysis, such as FT-IR, NMR and mass spectroscopy. These new compounds were tested for their antiproliferative activities on seven representative human tumoral cell lines (Huh7 D12, Caco2, MDA-MB231, MDA-MB468, HCT116, PC3 and MCF7) and also on fibroblasts. Among them, only the compounds 6c showed micromolar cytotoxic activity on tumor cell lines (1.8 50 50 > 25 μM). Finally, in silico ADMET studies ware performed to investigate the possibility of using of the identified compound 6c as potential anti-tumor compound.
文摘This article is based on a recent model specifically defining magnetic field values around electrical wires. With this model, calculations of field around parallel wires were obtained. Now, this model is extended with the new concept of magnetic equipotential surface to magnetic field curves around crossing wires. Cases of single, double, and triple wires are described. Subsequent article will be conducted for more general scenarios where wires are neither infinite nor parallel.
文摘In recent years,with the introduction of the strategy to strengthen the country’s transportation infrastructure and the continuous implementation of new economic development models such as integrating transportation with tourism,various open service areas integrating highway service areas with local tourism have emerged nationwide.Examples include the Yangcheng Lake service area in Jiangsu and the Lengshui service area in Chongqing.This paper focuses on the design example of the Dazu Stone Carving service area on the Chongqing section of the Yurong Expressway,comprehensively considering factors such as construction scale,terrain conditions,and local urban planning.It analyzes and studies the traffic flow design of unilateral agglomerative open service areas around two vehicle traffic conversion links:between the main expressway and the service area,and between the service area and local roads.The aim is to provide a case study reference for similar projects.
基金funded by the German Research Council(DFG grant MA6473/2-1,awarded to AM)The Mason lab is partially funded by the Deutsche Forschungsgemeinschaft(DFG,German Research Foundation)under Germany’s Excellence Strategy-EXC 2070-390732324.
文摘Rapeseed(Brassica napus,AACC)was formed by hybridization between progenitor species Brassica rapa(AA)and Brassica oleracea(CC).As a result of a limited number of hybridization events between specific progenitor genotypes and strong breeding selection for oil quality traits,rapeseed has limited genetic diversity.The production of resynthesized B.napus lines via interspecific hybridization of the diploid progenitor species B.rapa and B.oleracea is one possible way to increase genetic variation in rapeseed.However,most resynthesized lines produced so far have been reported to be meiotically unstable and infertile,in contrast to established B.napus cultivars.This hinders both maintenance and use of this germplasm in breeding programs.We characterized a large set of 140 resynthesized lines produced by crosses between B.rapa and B.oleracea,as well as between B.rapa and wild C genome species(B.incana,B.hilarionis,B.montana,B.Bourgeaui,B.villosa and B.cretica)for purity(homozygosity),fertility,and genome stability.Self-pollinated seed set,seeds per ten pods as well as percentage pollen viability were used to estimate fertility.SNP genotyping was performed using the Illumina Infinium Brassica 60K array for 116 genotypes,with at least three individuals per line.Most of the material which had been advanced through multiple generations was no longer pure,with heterozygosity detected corresponding to unknown parental contributions via outcrossing.Fertility and genome stability were both genotypedependent.Most lines had high numbers of copy number variants(CNVs),indicative of meiotic instability,and high numbers of CNVs were significantly associated with reduced fertility.Eight putatively stable resynthesized B.napus lines were observed.Further investigation of these lines may reveal the mechanisms underlying this effect.Our results suggest that selection of stable resynthesized lines for breeding purposes is possible.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.61571018 and 61531003).
文摘Micrometric-thin cells(MCs)with alkali vapor atoms have been valuable for research and applications of hyperfine Zeeman splitting and atomic magnetometers under strong magnetic fields.We theoretically and experimentally study the saturated absorption spectra using a 100-μm cesium MC,where the pump and probe beams are linearly polarized with mutually perpendicular polarizations,and the magnetic field is along the pump beam.Because of the distinctive thin chamber of the MC,crossover spectral lines in saturated absorption spectra are largely suppressed leading to clear splittings of hyperfine Zeeman transitions in experiments,and the effect of spatial magnetic field gradient is expected to be reduced.A calculation method is proposed to achieve good agreements between theoretical calculations and experimental results.This method successfully explains the suppression of crossover lines in MCs,as well as the effects of magnetic field direction,propagation and polarization directions of the pump/probe beam on saturated absorption spectrum.The saturated absorption spectrum with suppressed crossover lines is used for laser frequency stabilization,which may provide the potential value of MCs for high spatial resolution strong-field magnetometry with high sensitivity.
基金supported by the National Natural Science Foundation of China under Grants 62362040,61662033supported by the Science and Technology Project of the State Grid Jiangxi Electric Power Co.,Ltd.of China under Grant 521820210006.
文摘The YOLOx-s network does not sufficiently meet the accuracy demand of equipment detection in the autonomous inspection of distribution lines by Unmanned Aerial Vehicle(UAV)due to the complex background of distribution lines,variable morphology of equipment,and large differences in equipment sizes.Therefore,aiming at the difficult detection of power equipment in UAV inspection images,we propose a multi-equipment detection method for inspection of distribution lines based on the YOLOx-s.Based on the YOLOx-s network,we make the following improvements:1)The Receptive Field Block(RFB)module is added after the shallow feature layer of the backbone network to expand the receptive field of the network.2)The Coordinate Attention(CA)module is added to obtain the spatial direction information of the targets and improve the accuracy of target localization.3)After the first fusion of features in the Path Aggregation Network(PANet),the Adaptively Spatial Feature Fusion(ASFF)module is added to achieve efficient re-fusion of multi-scale deep and shallow feature maps by assigning adaptive weight parameters to features at different scales.4)The loss function Binary Cross Entropy(BCE)Loss in YOLOx-s is replaced by Focal Loss to alleviate the difficulty of network convergence caused by the imbalance between positive and negative samples of small-sized targets.The experiments take a private dataset consisting of four types of power equipment:Transformers,Isolators,Drop Fuses,and Lightning Arrestors.On average,the mean Average Precision(mAP)of the proposed method can reach 93.64%,an increase of 3.27%.The experimental results show that the proposed method can better identify multiple types of power equipment of different scales at the same time,which helps to improve the intelligence of UAV autonomous inspection in distribution lines.
文摘The classical minimization of power losses in transmission lines is dominated by artificial intelligence techniques, which do not guarantee global optimum amidst local minima. Revolutionary and evolutionary techniques are encumbered with sophisticated transformations, which weaken the techniques. Power loss minimization is crucial to the efficient design and operation of power transmission lines. Minimization of losses is one way to meet steady grid supply, especially at peak demand. Thus, this paper has presented a gradient technique to obtain optimal variables and values from the power loss model, which efficiently minimizes power losses by modifying the traditional power loss model that combines Ohm and Corona losses. Optimality tests showed that the unmodified model does not support the minimization of power losses on transmission lines as the Hessian matrix portrayed the maximization of power losses. However, the modified model is consistent with the gradient method of optimization, which yielded optimum variables and values from the power loss model developed in this study. The unmodified (modified) models for Bujagali-Kawanda 220 kV and Masaka West-Mbarara North 132 kV transmission lines in Uganda showed maximum power losses of 0.406 (0.391) and 0.452 (0.446) kW/km/phase respectively. These results indicate that the modified model is superior to the unmodified model in minimizing power losses in the transmission lines and should be implemented for the efficient design and operation of power transmission lines within and outside Uganda for the same transmission voltages.
文摘In this paper, we have studied several classes of planar piecewise Hamiltonian systems with three zones separated by two parallel straight lines. Firstly, we give the maximal number of limit cycles in these classes of systems with a center in two zones and without equilibrium points in the other zone (or with a center in one zone and without equilibrium points in the other zones). In addition, we also give examples to illustrate that it can reach the maximal number.
文摘A miniaturized broadband Wilkinson power divider is proposed. Micro-strip branch lines are introduced to replace multiple resistors used in multi-stage Wilkinson power dividers to increase the bandwidth of single-stage Wilkinson power dividers. To demonstrate its performance, an improved single-stage Wilkinson power divider with four micro-strip branch lines was designed. Simulated results show that the insert loss is better than 3.2 dB, the input return loss, output return loss, and isolation are better than 15 dB respectively, across a 76% bandwidth from 18 to 40 GHz. .
基金supported by the National Natural Science Foundation of China(32171990 and 32072053)Key Research and Development Program of Zhenjiang(NY2021001)+4 种基金State Key Laboratory of Plant Cell and Chromosome Engineering(PCCE-KF-2021-05 and PCCE-KF-2022-07)State Key Laboratory of Crop Biology in Shandong Agricultural University(2021KF01)Natural Science Foundation of the Jiangsu Higher Education institutions of China(21KJB210004)Open Project Funding of State Key Laboratory of Crop Stress Adaptation and Improvement(CX1130A0920014)Key Research and Development Program of Shandong Province(2020CXGC010805).
文摘Rye(Secale cereale)is a valuable gene donor for wheat improvement,especially for its resistance to diseases.Developing rye-derived resistance sources is important for wheat breeding.In the present study,two wheat-rye derivatives,designated JS016 and JS110,were produced by crossing common wheat cultivar Yangmai 23 with Pakistani rye accession W2A.Using sequential genomic in situ hybridization(GISH)and multicolor fluorescence in situ hybridization(mc-FISH),JS016 and JS110 were identified as a T6BS.6RL translocation line and a T6BS.6BL6RL translocation line,respectively.Ten newly 6RL chromosome arm-specific markers were developed and used to confirm the 6RL translocation.The wheat 55K single-nucleotide polymorphism(SNP)array further verified the molecular cytogenetic identification results above and clarified their breakpoints at 430.9 and 523.0 Mb of chromosome 6B in JS016 and JS110,respectively.Resistance spectrum and allelism test demonstrated that JS016 and JS110 possessed novel powdery mildew resistance gene(s)that was derived from the 6RL translocation but differed from Pm20.Moreover,JS016 and JS110 had better agronomic traits than the previously reported 6RL translocation line carrying Pm20.To efficiently transfer and detect the 6RL translocation from JS016 and JS110,one 6RL-specific Kompetitive allele specific PCR(KASP)marker was developed and validated in high throughput marker-assisted selection(MAS).
基金funded by the Basic Scientific Research Special Project for Fujian Provincial Public Research Institutes(2020R10310011,2019R1031-12)the National Natural Science Foundation of China(32001577)+4 种基金the Foreign Cooperation Projects for FAAS(DWHZ2021-20)the Opening Foundation of Fujian Key Laboratory for Vegetable Breeding(FJVRC2020-02)the Free Explore Program for FAAS(ZYTS2019007)the Guangxi Natural Science Foundation Program(2018GXNSFDA281027)the Science and Technology Innovative Team in Fujian Academy of Agricultural Sciences(CXTD2021008-3).
文摘Peanut(Arachis hypogaea L.)is an oil and economic crop of vital importance,and peanut pod is the key organ influencing the yield and processing quality.Hence,the Pod-related traits(PRTs)are considered as important agronomic traits in peanut breeding.To broaden the variability of PRTs in current peanut germplasms,three elite peanut cultivars were used to construct Ethyl methane sulfonate(EMS)-induced mutant libraries in this study.The optimal EMS treatment conditions for the three peanut varieties were determined.It was found that the median lethal dose(LD50)of EMS treatment varied greatly among different genotypes.Finally,the EMS-induced peanut mutant libraries were constructed and a total of 124 mutant lines for PRTs were identified and evaluated.Furthermore,“M-8070”,one of the mutant lines for pod constriction,was re-sequenced via high-throughput sequencing technology.The genome-wide variations between“M-8070”and its wild parent“Fuhua 8”(FH 8)were detected.2994 EMS-induced single nucleotide polymorphisms(SNPs)and 1188 insertion-deletions(InDels)between“M-8070”and its wild parent were identified.The predominant SNP mutation type was C/G to T/A transitions,while the predominant InDel mutation type was“1-bp”.We analyzed the distribution of identified mutations and annotated their functions.Most of the mutations(91.68%of the SNPs and 77.69%of the InDels)were located in the intergenic region.72 SNPs were identified in the exonic region,leading to 27 synonymous,43 nonsynonymous and 2 stop-gain variation for gene structure.13 Indels were identified in the exonic region,leading to 4 frame-shift,8 non-frame-shift and 1 stop-gain variations of genes.These mutations may lead to the phenotypic variation of“M-8070”.Our study provided valuable resources for peanut improvement and functional genomic research.
文摘Eight indica ( Oryza sativa L.) environment-sensitive genic male-sterile (EGMS) lines, 2-2S, K1405S, F131S, 2136S, Pei-Ai 64S, 1290S, GD-IS and N17S, were sequentially seeded with 10-15 d interval at three sites, Wuhan in 1997, Guiyang in 1997 and Sanya in 1997 and 1998, China. The results of investigations on self-sterilities showed that all of eight EGMS lines had stable sterile periods of longer than 30 d at Wuhan. They can be used for seed production of two-line hybrid rice, but can not reproduce themselves. Their stable sterile periods were shorter than 30 d at Guiyang, they can reproduce themselves and can not be used for hybrid seed production. In Sanya, their stable sterile periods were longer than 150 d, all of eight lines can be used for seed production in summer and autumn and reproduce themselves in winter. The fertility of all eight lines were sensitive to temperature. The sensitive stages, sensitive duration and critical point of temperatures (CPT) of fertility alteration in various lines were different. The sensitive stages of 2-2S and K1405S were from 18 d to 9 d before heading, the sensitive durations were 7-10 d and the CPTs were 23.7-24.5 degreesC. The sensitive stage, sensitive duration and CPT of F131S were from 17 to 5 d before heading, 13 d and 24.3-24.7 degreesC, respectively. The sensitive stage, sensitive duration and CPT of 2136S were from 18 to 12 d before heading, 7 d and 24.6-25.1 degreesC:, respectively. The sensitive stages, sensitive durations of Pei-Ai 64S, 1290S, N17S and GD-1S were from 24 to 13 d before heading and 10-13 d. And their CPTs were 24.6-25.1 degreesC, 25.5-26.2 degreesC, 25.4-26.1 degreesC,, and 24.1-24.7 degreesC, respectively.
基金Shanghai Foundation for Development of Industrial Internet Innovation,China (No. 2019-GYHLW-004)。
文摘Digital twin(DT) is a virtual replica of a physical world that has become one of the most important ideas in the manufacturing industry’s digital revolution. DT modeling is a vital issue in building a DT of a production line. In this paper, a method is proposed to address the difficulties of complicated production line business and data heterogeneity. The method focuses on essential data in the production line and creates conceptual and information models based on the ArtiFlow model and AutomationML(AML). Conceptual models are mainly used to describe and analyze the business activities of the production line, and information models describe real production lines in the form of XML files. The proposed modeling approach has been applied to a real-world clothing production line to demonstrate its feasibility and effectiveness.
文摘A total of 28 accessions of rice (Oryza sativa L.) germplasms were collected from International Rice Research Institute (IRRI), Philippine and Gene Pool of China National Rice Research Institute (CNRRI). These germplasms were attributed as a Donor Gene Pool, consisting of 27 diverse morphological marker lines with each having a unique valuable trait (gene), and the recurrent parent-Zhefu 802, an early-season indica cultivar in south of China in the 1980s and 1990s. A set of 27 isogenic lines with 2-3 markers on every chromosome were obtained after more than 10 successive backcrosses. Moreover, 15 accessions of double-marker isogenic lines were developed by further pyramiding markers on the same chromosome. Key agronomic traits such as heading date, plant height, tillering number and panicle length in this set of lines were similar to that of Zhefu 802 except the marker traits.
