AIM: To evaluate the relationship between the expression of lipopolyaaccharides (LPS) binding protein (LBP) and CD14mRNA and the severity of liver injury in alcohol-fed rats.METHODS: Twenty Wietar rats were divided i...AIM: To evaluate the relationship between the expression of lipopolyaaccharides (LPS) binding protein (LBP) and CD14mRNA and the severity of liver injury in alcohol-fed rats.METHODS: Twenty Wietar rats were divided into two groups: ethanol-fed group (group E) and control group (group C). Group E was fed with ethanol( 5-12g. Kg- 1. D-1)and group C received dextrose instead of ethanol. Rats of the two groups were sacrificed at 4 weeks and 8 weeks.Levels of endotoxin and alanine transeminase (ALT) inblood were measured, and liver pathology was observed under light and electronic microscopy. Expressions of LBP and CD14 mRNA in liver tissues were determined by RT-PCR analysis.RESULTS: Plasma endotoxin levels were increased more significantly in group E( 129 ± 21) ng. L- 1 and ( 187 ± 35) ng.L- 1 at 4 and 8 wk than in control rats(48 ± 9) ng. L- 1 and (53±11) ng.L-1, respectively (P< 0.05). Mean values of plasma ALT levels were (1867 ± 250) nkat. L-1 and (2450 ±367) nkat. L- 1 in Group E. The values were increased more dramatically in ethanol-fed rats than in Group C after 4 and 8weeks. In liver section from ethanol-fed rats, there were marked pathological changes (steatosis, cell infiltration and necrosis). In ethanol-fed rats, ethanol administration led to a significant increase in LBP and CD14 mRNA levels compared with the control group ( P< 0.05).CONCLUSION: Ethanol administration led to a significant increase in endotoxin levels in serum and LBP and CD14mRNA expressions in liver tissues. The increase of LBP and CD14 mRNA expression might wake the liver more sensitive to endotoxin and liver injury.展开更多
INTRODUCTIONInflammatory pseudotumor of the liver ,a rare benign lesion ,is often confused with the malignant tumors .Until 1998,less than 80 cases had been reported in the world [1,2].The accuracy of the pre-surgica...INTRODUCTIONInflammatory pseudotumor of the liver ,a rare benign lesion ,is often confused with the malignant tumors .Until 1998,less than 80 cases had been reported in the world [1,2].The accuracy of the pre-surgical diagnosis was low p[3,4] ,and very few materials on the diagnosis using dynamic MRI were reported [5-7] .We collected thirteen cases of inflammatory pseudotumor of the liver were collected and roved by pathology with MRI studies .Our purpose is to describe and analyze the MRI findings of this lesion and find out the valuable signs suggesting the diagnosis so as to avoid unnecessary operations.展开更多
AIM To study the effects of hypoxia, hyperoxia on the regulation of expression and activity of matrix metalloproteinase-2 (MMP-2) in hepatic stellate cells ( HSC).``METHODS The expressions of MMP-2, tissue inhibitor o...AIM To study the effects of hypoxia, hyperoxia on the regulation of expression and activity of matrix metalloproteinase-2 (MMP-2) in hepatic stellate cells ( HSC).``METHODS The expressions of MMP-2, tissue inhibitor of mjatrix metalloproteinese-2 ( TIMP-2 ) and membrane type matrix metalloproteiness-l (MT1-MMP) in cultured rat HSC were detected by immunocytochemistry (ICC) and in situ hybridization (ISH). The contents of MMP-2 and TIMP-2 in culture supernatant were detected with ELISA and the activity of MMP-2 in supematant was revealed by zymography.``RESULTS In the situation of hypoxia for 12 h, the expression of MMP-2 protein wss enhenced (hypoxiagroup positive indexes: 5.7 ± 2.0, n = 10; control: 3.2 ±1 .0. n -- 7; P＜0.05). while TIMP-2 protein wss decreased in HSC ( hypoxia group positive indexes: 2.5 ± 0.7, n =10: control: 3.6 ± 1.0, n = 7; P＜O.05), and the activity ( total A) of MMP-2 in supematant declined obviously (hypoxia group: 7.334 ± 1.922, n = 9; control: 17.277 ±7.424. n= 11; P＜0.01). Compared the varied duration of hypoxia, the changes of expressions including mRNA and protein level as well as activity of MMP-2 were most notable in 6 h group. The highest value (Ahypoxia-Acontrol) ofthe protein and the most intense signal of mRNA were in the period of hypoxia for 6 h, along with the lowest activity of MMP-2. In the situation of hyperoxia for 12 h,the contonts (A450) of MMP-2 and TIMP-2 in supernatant were both higher then those in the control, especially the TIMP-2 (hyperoxia group: 0.0499 _+ 0.0144, n = 16;control: 0.0219 ± 0.0098, n = 14; P＜0.01), and so was the activity of MMP-2 (hyperoxia group: 5.252 _+ 0.771,n: 14; control: 4.304 +_ 1 .083, n = 12; P＜0.05), and the expression of MT1-MMP was increased.``CONCLUSION HSC Js sensitive to the oxygen, hypoxia enhances the expression of MMP-2 and the effect is more marked at the early stage; hyperoxia mainly raises the activity of MMP-2.展开更多
基金Supported by the National Natural Science Foundation of China(No.39970719).
文摘AIM: To evaluate the relationship between the expression of lipopolyaaccharides (LPS) binding protein (LBP) and CD14mRNA and the severity of liver injury in alcohol-fed rats.METHODS: Twenty Wietar rats were divided into two groups: ethanol-fed group (group E) and control group (group C). Group E was fed with ethanol( 5-12g. Kg- 1. D-1)and group C received dextrose instead of ethanol. Rats of the two groups were sacrificed at 4 weeks and 8 weeks.Levels of endotoxin and alanine transeminase (ALT) inblood were measured, and liver pathology was observed under light and electronic microscopy. Expressions of LBP and CD14 mRNA in liver tissues were determined by RT-PCR analysis.RESULTS: Plasma endotoxin levels were increased more significantly in group E( 129 ± 21) ng. L- 1 and ( 187 ± 35) ng.L- 1 at 4 and 8 wk than in control rats(48 ± 9) ng. L- 1 and (53±11) ng.L-1, respectively (P< 0.05). Mean values of plasma ALT levels were (1867 ± 250) nkat. L-1 and (2450 ±367) nkat. L- 1 in Group E. The values were increased more dramatically in ethanol-fed rats than in Group C after 4 and 8weeks. In liver section from ethanol-fed rats, there were marked pathological changes (steatosis, cell infiltration and necrosis). In ethanol-fed rats, ethanol administration led to a significant increase in LBP and CD14 mRNA levels compared with the control group ( P< 0.05).CONCLUSION: Ethanol administration led to a significant increase in endotoxin levels in serum and LBP and CD14mRNA expressions in liver tissues. The increase of LBP and CD14 mRNA expression might wake the liver more sensitive to endotoxin and liver injury.
文摘INTRODUCTIONInflammatory pseudotumor of the liver ,a rare benign lesion ,is often confused with the malignant tumors .Until 1998,less than 80 cases had been reported in the world [1,2].The accuracy of the pre-surgical diagnosis was low p[3,4] ,and very few materials on the diagnosis using dynamic MRI were reported [5-7] .We collected thirteen cases of inflammatory pseudotumor of the liver were collected and roved by pathology with MRI studies .Our purpose is to describe and analyze the MRI findings of this lesion and find out the valuable signs suggesting the diagnosis so as to avoid unnecessary operations.
基金Supported by the Scientific Research Fund for Doctorate Education,State Educational Commission,No.9837
文摘AIM To study the effects of hypoxia, hyperoxia on the regulation of expression and activity of matrix metalloproteinase-2 (MMP-2) in hepatic stellate cells ( HSC).``METHODS The expressions of MMP-2, tissue inhibitor of mjatrix metalloproteinese-2 ( TIMP-2 ) and membrane type matrix metalloproteiness-l (MT1-MMP) in cultured rat HSC were detected by immunocytochemistry (ICC) and in situ hybridization (ISH). The contents of MMP-2 and TIMP-2 in culture supernatant were detected with ELISA and the activity of MMP-2 in supematant was revealed by zymography.``RESULTS In the situation of hypoxia for 12 h, the expression of MMP-2 protein wss enhenced (hypoxiagroup positive indexes: 5.7 ± 2.0, n = 10; control: 3.2 ±1 .0. n -- 7; P＜0.05). while TIMP-2 protein wss decreased in HSC ( hypoxia group positive indexes: 2.5 ± 0.7, n =10: control: 3.6 ± 1.0, n = 7; P＜O.05), and the activity ( total A) of MMP-2 in supematant declined obviously (hypoxia group: 7.334 ± 1.922, n = 9; control: 17.277 ±7.424. n= 11; P＜0.01). Compared the varied duration of hypoxia, the changes of expressions including mRNA and protein level as well as activity of MMP-2 were most notable in 6 h group. The highest value (Ahypoxia-Acontrol) ofthe protein and the most intense signal of mRNA were in the period of hypoxia for 6 h, along with the lowest activity of MMP-2. In the situation of hyperoxia for 12 h,the contonts (A450) of MMP-2 and TIMP-2 in supernatant were both higher then those in the control, especially the TIMP-2 (hyperoxia group: 0.0499 _+ 0.0144, n = 16;control: 0.0219 ± 0.0098, n = 14; P＜0.01), and so was the activity of MMP-2 (hyperoxia group: 5.252 _+ 0.771,n: 14; control: 4.304 +_ 1 .083, n = 12; P＜0.05), and the expression of MT1-MMP was increased.``CONCLUSION HSC Js sensitive to the oxygen, hypoxia enhances the expression of MMP-2 and the effect is more marked at the early stage; hyperoxia mainly raises the activity of MMP-2.
