This paper proposes luteolin(LUT)as a novel depressant for the flotation-based separation of scheelite and calcite in a sodium oleate(NaOL)system.The suitability of LUT as a calcite depressant is confirmed through mic...This paper proposes luteolin(LUT)as a novel depressant for the flotation-based separation of scheelite and calcite in a sodium oleate(NaOL)system.The suitability of LUT as a calcite depressant is confirmed through micro-flotation testing.At pH=9,with LUT concentration of 50 mg·L^(-1) and NaOL concentration of 50 mg·L^(-1),scheelite recovery reaches 80.3%.Calcite,on the other hand,exhibits a recovery rate of 17.6%,indicating a significant difference in floatability between the two minerals.Subsequently,the surface modifica-tions of scheelite and calcite following LUT treatment are characterized using adsorption capacity testing,Zeta potential analysis,Fourier transform infrared spectroscopy(FT-IR),X-ray photoelectron spectroscopy(XPS),and atomic force microscopy(AFM).The study in-vestigates the selective depressant mechanism of LUT on calcite.Adsorption capacity testing and Zeta potential analysis demonstrate sub-stantial absorption of LUT on the surface of calcite,impeding the further adsorption of sodium oleate,while its impact on scheelite is min-imal.FT-IR and XPS analyses reveal the selective adsorption of LUT onto the surface of calcite,forming strong chemisorption bonds between the hydroxyl group and calcium ions present.AFM directly illustrates the distinct adsorption densities of LUT on the two miner-al types.Consequently,LUT can effectively serve as a depressant for calcite,enabling the successful separation of scheelite and calcite.展开更多
Background Oxidative stress,caused by an imbalance in the production and elimination of intracellular reactive oxygen species(ROS),has been recognized for its detrimental effects on mammalian embryonic development.Lut...Background Oxidative stress,caused by an imbalance in the production and elimination of intracellular reactive oxygen species(ROS),has been recognized for its detrimental effects on mammalian embryonic development.Luteolin(Lut)has been documented for its protective effects against oxidative stress in various studies.However,its specific role in embryonic development remains unexplored.This study aims to investigate the influence of Lut on porcine embryonic development and to elucidate the underlying mechanism.Results After undergoing parthenogenetic activation(PA)or in vitro fertilization,embryos supplemented with 0.5μmol/L Lut displayed a significant enhancement in cleavage and blastocyst formation rates,with an increase in total cell numbers and a decrease in the apoptosis rate compared to the control.Measurements on D2 and D6 revealed that embryos with Lut supplementation had lower ROS levels and higher glutathione levels compared to the control.Moreover,Lut supplementation significantly augmented mitochondrial content and membrane potential.Intriguingly,activation of the Nrf2/Keap1 signaling pathway was observed in embryos supplemented with Lut,leading to the upregulation of antioxidant-related gene transcription levels.To further validate the relationship between the Nrf2/Keap1 signaling pathway and effects of Lut in porcine embryonic development,we cultured PA embryos in a medium supplemented with brusatol,with or without the inclusion of Lut.The positive effects of Lut on developmental competence were negated by brusatol treatment.Conclusions Our findings indicate that Lut-mediated activation of the Nrf2/Keap1 signaling pathway contributes to the enhanced production of porcine embryos with high developmental competence,and offers insight into the mechanisms regulating early embryonic development.展开更多
Objective:To investigate the renoprotective effects of luteolin on diabetes in rats.Methods:One week after administration of streptozotocin 55 mg/kg intraperitoneally,rats were given 25,50,and 75 mg/kg/day of luteolin...Objective:To investigate the renoprotective effects of luteolin on diabetes in rats.Methods:One week after administration of streptozotocin 55 mg/kg intraperitoneally,rats were given 25,50,and 75 mg/kg/day of luteolin orally for another eight weeks.At the end of the experiment,body weight,blood glucose level,biochemical parameters for renal function(serum creatinine,blood urea nitrogen,uric acid,serum albumin,and total protein),kidney histology,matrix metalloproteinase(MMP)-2,MMP-9,and histone deacetylase 2(HDAC-2)expression,and malondialdehyde,myeloperoxidase,and hydroxyproline content in renal tissue were evaluated.High glucose-induced damage using NRK-52E cell line was studied to evaluate cell viability and metalloenzyme expression.Additionally,in silico studies including docking and molecular dynamics simulations were conducted.Results:MMP-2,MMP-9,and HDAC-2 expressions were significantly increased in high glucose-induced NRK-52E cells and the renal tissue of diabetic rats.However,these changes were reversed by luteolin at the administered doses.Additionally,luteolin significantly reduced oxidative stress,inflammation,and fibrosis,as well as improved biochemical parameters in diabetic rats.Furthermore,luteolin at the examined doses markedly alleviated diabetes-induced histopathological changes in renal tissues.Conclusions:Luteolin effectively attenuates streptozotocin-induced diabetic nephropathy in rats by inhibiting MMP-2,MMP-9,and HDAC-2 expression,and reducing oxidative stress and inflammation.展开更多
Dietary flavonoids are abundant in natural plants and possess multiple pharmacological and nutritional activities.In this study,apigenin,luteolin,and baicalein were chosen to evaluate their anti-diabetic effect in hig...Dietary flavonoids are abundant in natural plants and possess multiple pharmacological and nutritional activities.In this study,apigenin,luteolin,and baicalein were chosen to evaluate their anti-diabetic effect in high-glucose and dexamethasone induced insulin-resistant(IR)HepG2 cells.All flavonoids improves the glucose consumption and glycogen synthesis abilities in IR-HepG2 cells via activating glucose transporter protein 4(GLUT4)and phosphor-glycogen synthase kinase(GSK-3β).These fl avonoids signifi cantly inhibited the production of reactive oxygen species(ROS)and advanced glycation end-products(AGEs),which were closely related to the suppression of the phosphorylation form of NF-κB and P65.The expression levels of insulin receptor substrate-1(IRS-1),insulin receptor substrate-2(IRS-2)and phosphatidylinositol 3-kinase(PI3K)/protein kinase B(Akt)pathway in IR-HepG2 cells were all partially activated by the fl avonoids,with variable effects.Furthermore,the intracellular metabolic conditions of the fl avonoids were also evaluated.展开更多
Background:Patients diagnosed with ulcerative colitis(UC)are known to have an increased susceptibility to colorectal cancer(CRC).However,the shared underlying mechanisms between UC and CRC remain unclear.Given the the...Background:Patients diagnosed with ulcerative colitis(UC)are known to have an increased susceptibility to colorectal cancer(CRC).However,the shared underlying mechanisms between UC and CRC remain unclear.Given the therapeutic potential of luteolin in both UC and CRC,this study aims to elucidate the molecular targets and mechanisms through which luteolin exerts its effects against these diseases.Methods:The GeneCards database,DisGENet database,and Gene Expression Omnibus database were utilized to analyze the targets associated with UC and CRC.Subsequently,the Traditional Chinese Medicine Systems Pharmacology and SwissTargetPrediction databases were employed to identify luteolin-related targets.The identified luteolin-related targets were then mapped to official gene symbols using the UniProt database.The Cytoscape 3.9.0 software was utilized to construct a network of luteolin-associated targets.Venn diagram analysis was performed to identify common targets among UC,CRC,and luteolin.The common targets were further analyzed using the STRING database to construct a protein-protein interaction network.The“cytoHubba”plugin in Cytoscape 3.9.0 was employed to identify hub targets within the PPI network.Gene Ontology functional analysis and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis were conducted on the hub targets.Finally,molecular docking using AutoDock and PyMOL software was performed to assess the binding affinity between luteolin and the hub targets.Results:Luteolin was found to interact with a total of 149 pharmacological targets,while UC and CRC were associated with 1232 and 3278 targets,respectively.Forty-six common targets were identified among luteolin,UC,and CRC.Through the application of seven different algorithms,seven hub targets were identified,TP53,AKT1,TNF,SRC,EGFR,and MMP9.Bioinformatics enrichment analysis revealed 49 enriched pathways through Kyoto Encyclopedia of Genes and Genomes analysis,while Gene Ontology analysis yielded a total of 245 biological processes,4 cellular components,and 7 molecular functions.Molecular docking simulations demonstrated a good binding affinity between luteolin and the hub targets.Conclusion:This study identified multiple potential pharmacological targets and elucidated various biological pathways through which luteolin may exert its therapeutic effects in the treatment of UC and CRC.These findings provide a solid theoretical foundation for further experimental investigations in the treatment of UC and CRC.展开更多
Objective:To obtain Iuteolin and apigenin rich fraction from the ethanolic extract of Cynodon dactylon(L.)(C.dactylon) Pers and evaluate the fraction's cytotoxicity and anti-Chikungunya potential using Vero cells....Objective:To obtain Iuteolin and apigenin rich fraction from the ethanolic extract of Cynodon dactylon(L.)(C.dactylon) Pers and evaluate the fraction's cytotoxicity and anti-Chikungunya potential using Vero cells.Methods:The ethanolic extract of C.dactylon was subjected to silica gel column chromatography to obtain anti-chikungunya virus(CHIKV) fraction.Reverse phase-HPLC and GC-MS studies were carried out to identily the major phytochemicals in the fraction using phylochemical standards.Cytotoxicity and the potential of the fraction against CHIKV were evaluated in vitro using Vero cells.Reduction in viral replication was assessed by reverse transcriptase-polymerase chain reaction(RT-PCR) after treating the viral infected Vero cells with the fraction.Results:Reverse Phase-HPLC and GC-MS studies confirmed the presence of flavonoids,luteolin and apigenin as major phytochemicals in the anti-CHIKV ethanolic fraction of C.dactylon- The fraction was found to exhibit potent viral inhibitory activity(about 98%) at the concentration of 50 μg/mL as observed by reduction in cytopathic effect,and the cytotoxic concentration of the fraction was found to be 250 μg/mL.RT-PCR analyses indicated that the reduction in viral mRNA synthesis in fraction treated infected cells was much higher than the viral infected control cells.Conclusions:Luteolin and apigenin rich ethanolic fraction from C.dactylon can be utilized as a potential therapeutic agent against CHIKV infection as the fraction does not show cytotoxicity while inhibiting the virus.展开更多
Studies have shown that the natural flavonoid luteolin has neurotrophic activity. In this study, we investigated the effect of luteolin in a mouse model of Down syndrome. Ts65 Dn mice, which are frequently used as a m...Studies have shown that the natural flavonoid luteolin has neurotrophic activity. In this study, we investigated the effect of luteolin in a mouse model of Down syndrome. Ts65 Dn mice, which are frequently used as a model of Down syndrome, were intraperitoneally injected with 10 mg/kg luteolin for 4 consecutive weeks starting at 12 weeks of age. The Morris water maze test was used to evaluate learning and memory abilities, and the novel object recognition test was used to assess recognition memory. Immunohistochemistry was performed for the neural stem cell marker nestin, the astrocyte marker glial fibrillary acidic protein, the immature neuron marker DCX, the mature neuron marker NeuN, and the cell proliferation marker Ki67 in the hippocampal dentate gyrus. Nissl staining was used to observe changes in morphology and to quantify cells in the dentate gyrus. Western blot assay was used to analyze the protein levels of brain-derived neurotrophic factor(BDNF) and phospho-extracellular signal-regulated kinase 1/2(p-ERK1/2) in the hippocampus. Luteolin improved learning and memory abilities as well as novel object recognition ability, and enhanced the proliferation of neurons in the hippocampal dentate gyrus. Furthermore, luteolin increased expression of nestin and glial fibrillary acidic protein, increased the number of DCX^+ neurons in the granular layer and NeuN^+ neurons in the subgranular region of the dentate gyrus, and increased the protein levels of BDNF and p-ERK1/2 in the hippocampus. Our findings show that luteolin improves behavioral performance and promotes hippocampal neurogenesis in Ts65 Dn mice. Moreover, these effects might be associated with the activation of the BDNF/ERK1/2 pathway.展开更多
Polyphenols are the main active components of the anti-inflammatory compounds in dandelion,and chlorogenic acid(CGA)is one of the primary polyphenols.However,the molecular mechanism underlying the transcriptional regu...Polyphenols are the main active components of the anti-inflammatory compounds in dandelion,and chlorogenic acid(CGA)is one of the primary polyphenols.However,the molecular mechanism underlying the transcriptional regulation of CGA biosynthesis remains unclear.Hydroxycinnamoyl-CoA:quinate hydroxycinnamoyl transferase(HQT2)is the last rate-limiting enzyme in chlorogenic acid biosynthesis in Taraxacum antungense.Therefore,using the TaHQT2 gene promoter as a probe,a yeast one-hybrid library was performed,and a basic helix-loop-helix(bHLH)transcription factor,TabHLH1,was identified that shared substantial homology with Gynura bicolor DC bHLH1.The TabHLH1 transcript was highly induced by salt stress,and the TabHLH1 protein was localized in the nucleus.CGA and luteolin concentrations in TabHLH1-overexpression transgenic lines were significantly higher than those in the wild type,while CGA and luteolin concentrations in TabHLH1-RNA interference(RNAi)transgenic lines were significantly lower.Quantitative real-time polymerase chain reaction demonstrated that overexpression and RNAi of TabHLH1 in T.antungense significantly affected CGA and luteolin concentrations by upregulating or downregulating CGA and luteolin biosynthesis pathway genes,especially TaHQT2,4-coumarate-CoA ligase(Ta4CL),chalcone isomerase(TaCHI),and flavonoid-3′-hydroxylase(TaF3′H).Dual-luciferase,yeast one-hybrid,and electrophoretic mobility shift assays indicated that TabHLH1 directly bound to the bHLH-binding motifs of proTaHQT2 and proTa4CL.This study suggests that TabHLH1 participates in the regulatory network of CGA and luteolin biosynthesis in T.antungense and might be useful for metabolic engineering to promote plant polyphenol biosynthesis.展开更多
The present study was conducted to verify the influence of luteolin on apoptosis of Eca109 cells and to further investigate the possible mechanisms underlying its effect on apoptosis.The cells were exposed to differen...The present study was conducted to verify the influence of luteolin on apoptosis of Eca109 cells and to further investigate the possible mechanisms underlying its effect on apoptosis.The cells were exposed to different concentrations of luteolin(0,40,80,120,160,200,240M)for 24,48,and 72 h respectively.The influence of luteolin on proliferation of Eca109 cells was detected using MTT assay.Eca109 cells were then treated with luteolin(0,40,160,240M)for 24 h.The effect of luteolin on cell cycle progression and apoptosis was assayed by using flow cytometry(FCM).Expression of caspase9 and caspase3 mRNA and protein was analyzed by real-time PCR and Western blot respectively.The results showed that luteolin could inhibit the proliferation of Eca109 cells at all concentrations in a time-dependent manner and the relative inhibition rate showed an inverted U-shaped association with the concentration of luteolin.Further,the cell cycle was arrested in the S phase following treatment with luteolin.Apoptosis analysis indicated that luteolin could induce the apoptosis of Eca109 cells across the three concentration groups,which exhibited a trend of first promotional and then inhibitory with the increases in luteolin concentration.The effect of luteolin on the mRNA and protein expression of caspase 9 and caspase3 first manifested as promotion,then inhibition.Therefore,luteolin may serve a role in promoting cell apoptosis by inducing Eca109 cell apoptosis that involves the expression of caspase3,caspase9 mRNA and protein.This study provides theoretical basis for further study and clinical application of luteolin.The specific mechanism has not yet been clarified and the other activation pathways inducing apoptosis need to be further studied.展开更多
AIM:To investigate the effects of luteolin on apoptosis,the cell cycle,and the expression and secretion of vascular endothelial growth factor(VEGF)in human choroidal melanoma cells(C918 and OCM-1).METHODS:C918 and OCM...AIM:To investigate the effects of luteolin on apoptosis,the cell cycle,and the expression and secretion of vascular endothelial growth factor(VEGF)in human choroidal melanoma cells(C918 and OCM-1).METHODS:C918 and OCM-1 cells cultured in vitro were treated with various concentrations of luteolin(0,5,10,15μmol/L).Cell growth was observed with an inverted microscope,and cell cycle arrest was detected by propidium iodide(PI)staining using flow cytometry.Apoptosis was detected by Hoechst33342 staining,and apoptosis rate was determined by Annexin V-FITC/PI experiments using flow cytometry.The expression of apoptosis-related proteins Bcl-2,Bax and VEGF was analyzed using Western blots.The levels of VEGF secreted by the cells into the supernatant was analyzed using ELISA.RESULTS:After treating with 5 to 15μmol/L luteolin for 48 h,the fusion degree of C918 and OCM-1 cells decreased,and more floating apoptotic cells appeared.Luteolin treatment increased the G0-G1 phase ratio of the C918 and OCM-1 cells,blocked cell cycle progression,and increased the apoptosis rate of the C918 and OCM-1 cells.Western blot showed that luteolin decreased the expression of Bcl-2 and VEGF in the C918 and OCM-1 cells and increased the expression of Bax protein.The ELISA results showed that 10 to 15μmol/L luteolin decreased the cell secretion of VEGF.CONCLUSION:Luteolin may induce apoptosis by regulating the levels of apoptosis-related proteins in C918 and OCM-1 cells.Luteolin can induce cell cycle arrest,decrease the expression of VEGF.展开更多
Luteolin is neuroprotective for retinal ganglion cells and retinal pigment epithelial cells after oxidative injury,whereby it can inhibit microglial neurotoxicity.Therefore,luteolin holds the potential to be useful fo...Luteolin is neuroprotective for retinal ganglion cells and retinal pigment epithelial cells after oxidative injury,whereby it can inhibit microglial neurotoxicity.Therefore,luteolin holds the potential to be useful for treatment of retinal diseases.The purpose of this study was to investigate whether luteolin exhibits neuroprotective effects on rod cells in rd10 mice,a slow photoreceptor-degenerative model of retinitis pigmentosa.Luteolin(100 mg/kg)intraperitoneally injected daily from postnatal day 14(P14)to P25 significantly enhanced the visual performance and retinal light responses of rd10 mice at P25.Moreover,it increased the survival of photoreceptors and improved retinal structure.Mechanistically,luteolin treatment attenuated increases in reactive oxygen species,photoreceptor apoptosis,and reactive gliosis;increased mRNA levels of anti-inflammatory cytokines while lowering that of pro-inflammatory and chemoattractant cytokines;and lowered the ratio of phospho-JNK/JNK.Application of the JNK inhibitor SP600125 exerted a similar protective effect to luteolin,suggesting that luteolin delays photoreceptor degeneration and functional deterioration in rd10 mice through regulation of retinal oxidation and inflammation by inhibiting the JNK pathway.Therefore,luteolin may be useful as a supplementary treatment for retinitis pigmentosa.This study was approved by the Qualified Ethics Committee of Jinan University,China(approval No.IACUC-20181217-02)on December 17,2018.展开更多
Natural dyestuff of luteolin was isolated and used to dye wool fabric in this paper. Ethanol extraction and high-speed countercurrent chromatography (HSCCC) were used to extract and purify the luteolin from the peanut...Natural dyestuff of luteolin was isolated and used to dye wool fabric in this paper. Ethanol extraction and high-speed countercurrent chromatography (HSCCC) were used to extract and purify the luteolin from the peanut shell, and the structure of the isolated luteolin was characterized with FTIR techniques. The interaction between dyestuff and fiber was preliminarily discussed through thermodynamic study and supramolecular structure simulation to explain the intrinsic reasons why the color fastness was low when luteolin was applied to dyeing wool fabric. The extraction condition and purification parameter were as follows: 65% ethanol, ratio of material to liquid 1:20, 80°C, 3 h, chloroform-methanol-water (4/3/2, V/V), 800 rmp/min, 2.0 Mkpa, 0.5 mL/ min and 280 nm. The results of dyeing thermodynamics showed that the sorption isotherm of luteolin on wool fabric was consistent with Nernst model and similar to the disperse dyestuff. With molecular simulation, luteolin and glycin composed 8 stable complexes whose Laplacian values all were greater than 0, which suggested typical hydrogen bonds existing. The complex with three hydrogen bonds was proved the most stable. Both studies on thermodynamics and supramolecular simulation revealed that luteolin on wool fabric mainly depended on the weak hydrogen bonds interaction that determined the low dyefastness.展开更多
Studies have indicated that flavonoid luteolin is a potential inhibitor of tumor cell proliferation and may function as an anticarcinogenic agent. Pyrrolidine dithiocarbamate (PDTC), a synthetic compound, may exhibit ...Studies have indicated that flavonoid luteolin is a potential inhibitor of tumor cell proliferation and may function as an anticarcinogenic agent. Pyrrolidine dithiocarbamate (PDTC), a synthetic compound, may exhibit biphasic effects on apoptosis depending on the experimental context. Previously, we found that luteolin induced the activation of the proapoptotic proteins, such as Bad, Bid, and Bax, in HL-60 human leukemia cells. We also explored the modulatory effects and molecular mechanisms of PDTC on the cytotoxicity of luteolin in HL-60 cells;PDTC could interfere with luteolin’s ability to cleave poly(ADP-ribose)-polymerase (PARP) and DNA fragmentation of factor-45 (DFF-45). In the current study, we further investigated the effect of PDTC on the luteolin-induced death-receptor pathway and the cleavage of the Bcl-2 family members. We found that the combination of luteolin and PDTC increased the survival of the HL-60 cells such that PDTC inhibited both extrinsic and intrinsic pathways in luteolin-induced apoptosis.展开更多
Objective This study aimed to investigate the effect of exposure to a 900 MHz electromagnetic field(EMF)on the cervical spinal cord(CSC)of rats and the possible protective effect of luteolin(LUT)against CSC tissue dam...Objective This study aimed to investigate the effect of exposure to a 900 MHz electromagnetic field(EMF)on the cervical spinal cord(CSC)of rats and the possible protective effect of luteolin(LUT)against CSC tissue damage.Methods Quantitative data were obtained via stereological,biochemical,immunohistochemical,and histopathological techniques.We investigated morphometric value,superoxide dismutase(SOD)level,and the expression of high-mobility group box 1 protein molecules,as well as histological changes.Results The total number of motor neurons in the EMF group significantly decreased in comparison with that in the control group(P<0.05).In the EMF+LUT group,we found a significant increase in the total number of motor neurons compared with that in the EMF group(P<0.05).SOD enzyme activity in the EMF group significantly increased in comparison with that in the control group(P<0.05).By contrast,the EMF+LUT group exhibited a decrease in SOD level compared with the EMF group(P<0.05).Conclusion Our results suggested that exposure to EMF could be deleterious to CSC tissues.Furthermore,the protective efficacy of LUT against SC damage might have resulted from the alleviation of oxidative stress caused by EMF.展开更多
[Objectives] This study was conducted to optimize the extraction process of luteolin, a natural product of Ternate Grape Fern Herb, so as to provide a theoretical research basis for the chemical composition, pharmacol...[Objectives] This study was conducted to optimize the extraction process of luteolin, a natural product of Ternate Grape Fern Herb, so as to provide a theoretical research basis for the chemical composition, pharmacological research and preparation development of Ternate Grape Fern Herb. [Methods] The effects of four factors including ultrasonic extraction time, liquid-to-material ratio, ultrasonic extraction temperature and ethanol concentration on extraction yield of luteolin from Ternate Grape Fern Herb were investigated by the ultrasonic-assisted extraction technology, through a single-factor experiment and an orthogonal experiment based on the single-factor experiment, and the experimental data was subjected to variance analysis and SSR analysis of the significance of differences between groups by SPSS20.0 software to select the optimal extraction process conditions. [Results] The optimal extraction process conditions were as follows: ultrasonic extraction time 25 min, liquid-to-material ratio 25∶1 ml/g, ethanol volume fraction 60%, ultrasonic extraction temperature 85 ℃. [Conclusions] The results were verified, and it was determined that the ultrasonic-assisted extraction process established in this study is reasonable and reliable, and can be used for the extraction of luteolin.展开更多
[Objectives]To determine the content of hyperin and luteolin in Vernonia cinerea(L.)Less.,and to provide basic experimental data for experimental research and clinical application of V.cinerea(L.)Less.[Methods]The com...[Objectives]To determine the content of hyperin and luteolin in Vernonia cinerea(L.)Less.,and to provide basic experimental data for experimental research and clinical application of V.cinerea(L.)Less.[Methods]The components to be determined were extracted by ultrasonic extraction,and the hyperin and luteolin in V.cinerea(L.)Less.were separated and determined by reversed-phase high performance liquid chromatography(chromatographic conditions:reverse C 18 column,methanol-0.4%phosphoric acid as mobile phase for gradient elution,flow rate of 1.0 mL/min,detector wavelength of 360 nm).[Results]The precision and repeatability of the experimental method were good,and the solution of V.cinerea(L.)Less.was stable within 18 h.There was a good linear relationship between the injection volume of hyperin and luteolin and the chromatographic peak area within the prescribed range,and the recovery rate was qualified.The content of hyperin and luteolin in V.cinerea(L.)Less.was 0.0665 mg/g(n=3)and 0.1004 mg/g(n=3),respectively.[Conclusions]The experimental method is sensitive,specific,stable,accurate and reliable,and can be used for the determination of hyperin and luteolin in V.cinerea(L.)Less.展开更多
Objective: To explore the anti-inflammatory effect of luteolin on rats with acute gout arthritis. Methods: A total of forty-eight rats were chosen and randomly divided into six groups. The acute gout arthritis model o...Objective: To explore the anti-inflammatory effect of luteolin on rats with acute gout arthritis. Methods: A total of forty-eight rats were chosen and randomly divided into six groups. The acute gout arthritis model of rats was established by injecting monosodium urate at the concentration of 25 mg/mL into the ankle joint cavity. Changes of joint swelling index at different time points and the levels of IL-1β, IL-17, TNF-α, IL-6 in serum and synovial were measured. Results: Compared with the control group, the swelling index of ankle joint and gait score in the model group was significantly enhanced at different time points (all P<0.01). Compared with the MSU group, luteolin improved the ankle swelling index of rats with acute gout arthritis (P<0.05), and significantly down-regulated the levels of IL-1β, IL-17, TNF-α, IL-6 (P<0.01). Conclusion: Luteolin can alleviate the inflammatory response of acute gouty arthritis and may be an effective drug for acute gouty arthritis.展开更多
基金supported by the National Natural Science Foundation of China (No.52164022).
文摘This paper proposes luteolin(LUT)as a novel depressant for the flotation-based separation of scheelite and calcite in a sodium oleate(NaOL)system.The suitability of LUT as a calcite depressant is confirmed through micro-flotation testing.At pH=9,with LUT concentration of 50 mg·L^(-1) and NaOL concentration of 50 mg·L^(-1),scheelite recovery reaches 80.3%.Calcite,on the other hand,exhibits a recovery rate of 17.6%,indicating a significant difference in floatability between the two minerals.Subsequently,the surface modifica-tions of scheelite and calcite following LUT treatment are characterized using adsorption capacity testing,Zeta potential analysis,Fourier transform infrared spectroscopy(FT-IR),X-ray photoelectron spectroscopy(XPS),and atomic force microscopy(AFM).The study in-vestigates the selective depressant mechanism of LUT on calcite.Adsorption capacity testing and Zeta potential analysis demonstrate sub-stantial absorption of LUT on the surface of calcite,impeding the further adsorption of sodium oleate,while its impact on scheelite is min-imal.FT-IR and XPS analyses reveal the selective adsorption of LUT onto the surface of calcite,forming strong chemisorption bonds between the hydroxyl group and calcium ions present.AFM directly illustrates the distinct adsorption densities of LUT on the two miner-al types.Consequently,LUT can effectively serve as a depressant for calcite,enabling the successful separation of scheelite and calcite.
基金supported by the Korea Research Institute of Bioscience and Biotechnology(KRIBB)Research Initiative Program(KGM4252331,KGM5382322),Republic of Korea.
文摘Background Oxidative stress,caused by an imbalance in the production and elimination of intracellular reactive oxygen species(ROS),has been recognized for its detrimental effects on mammalian embryonic development.Luteolin(Lut)has been documented for its protective effects against oxidative stress in various studies.However,its specific role in embryonic development remains unexplored.This study aims to investigate the influence of Lut on porcine embryonic development and to elucidate the underlying mechanism.Results After undergoing parthenogenetic activation(PA)or in vitro fertilization,embryos supplemented with 0.5μmol/L Lut displayed a significant enhancement in cleavage and blastocyst formation rates,with an increase in total cell numbers and a decrease in the apoptosis rate compared to the control.Measurements on D2 and D6 revealed that embryos with Lut supplementation had lower ROS levels and higher glutathione levels compared to the control.Moreover,Lut supplementation significantly augmented mitochondrial content and membrane potential.Intriguingly,activation of the Nrf2/Keap1 signaling pathway was observed in embryos supplemented with Lut,leading to the upregulation of antioxidant-related gene transcription levels.To further validate the relationship between the Nrf2/Keap1 signaling pathway and effects of Lut in porcine embryonic development,we cultured PA embryos in a medium supplemented with brusatol,with or without the inclusion of Lut.The positive effects of Lut on developmental competence were negated by brusatol treatment.Conclusions Our findings indicate that Lut-mediated activation of the Nrf2/Keap1 signaling pathway contributes to the enhanced production of porcine embryos with high developmental competence,and offers insight into the mechanisms regulating early embryonic development.
基金supported by the Joe,Pentti,and Tor Borg Memorial Fund.
文摘Objective:To investigate the renoprotective effects of luteolin on diabetes in rats.Methods:One week after administration of streptozotocin 55 mg/kg intraperitoneally,rats were given 25,50,and 75 mg/kg/day of luteolin orally for another eight weeks.At the end of the experiment,body weight,blood glucose level,biochemical parameters for renal function(serum creatinine,blood urea nitrogen,uric acid,serum albumin,and total protein),kidney histology,matrix metalloproteinase(MMP)-2,MMP-9,and histone deacetylase 2(HDAC-2)expression,and malondialdehyde,myeloperoxidase,and hydroxyproline content in renal tissue were evaluated.High glucose-induced damage using NRK-52E cell line was studied to evaluate cell viability and metalloenzyme expression.Additionally,in silico studies including docking and molecular dynamics simulations were conducted.Results:MMP-2,MMP-9,and HDAC-2 expressions were significantly increased in high glucose-induced NRK-52E cells and the renal tissue of diabetic rats.However,these changes were reversed by luteolin at the administered doses.Additionally,luteolin significantly reduced oxidative stress,inflammation,and fibrosis,as well as improved biochemical parameters in diabetic rats.Furthermore,luteolin at the examined doses markedly alleviated diabetes-induced histopathological changes in renal tissues.Conclusions:Luteolin effectively attenuates streptozotocin-induced diabetic nephropathy in rats by inhibiting MMP-2,MMP-9,and HDAC-2 expression,and reducing oxidative stress and inflammation.
基金supported by National Natural Science Foundation of China(32072212)Multi-Year Research Grant of University of Macao(MYRG2018-00169-ICMS)+5 种基金Science and Technology Development Fund of Macao(FDCT)(0098/2020/A)MICINN supporting the Ramón y Cajal grant for M.A.Prieto(RYC-201722891)Jianbo Xiao(RYC2020-030365-I)Xunta de Galicia supporting the Axudas Conecta Peme,the IN852A 2018/58 Neuro Food Project,the program EXCELENCIA-ED431F 2020/12the pre-doctoral grants of P.García-Oliveira(ED481A-2019/295)to Ibero-American Program on Science and Technology(CYTED-AQUA-CIBUS,P317RT0003).
文摘Dietary flavonoids are abundant in natural plants and possess multiple pharmacological and nutritional activities.In this study,apigenin,luteolin,and baicalein were chosen to evaluate their anti-diabetic effect in high-glucose and dexamethasone induced insulin-resistant(IR)HepG2 cells.All flavonoids improves the glucose consumption and glycogen synthesis abilities in IR-HepG2 cells via activating glucose transporter protein 4(GLUT4)and phosphor-glycogen synthase kinase(GSK-3β).These fl avonoids signifi cantly inhibited the production of reactive oxygen species(ROS)and advanced glycation end-products(AGEs),which were closely related to the suppression of the phosphorylation form of NF-κB and P65.The expression levels of insulin receptor substrate-1(IRS-1),insulin receptor substrate-2(IRS-2)and phosphatidylinositol 3-kinase(PI3K)/protein kinase B(Akt)pathway in IR-HepG2 cells were all partially activated by the fl avonoids,with variable effects.Furthermore,the intracellular metabolic conditions of the fl avonoids were also evaluated.
基金supported by the Key Research and Development Program of Shaanxi Province(2020ZDLSF05-02)the Key Science and Technology Project of New Agricultural Variety Breeding of Zhejiang Province(2021C02074).
文摘Background:Patients diagnosed with ulcerative colitis(UC)are known to have an increased susceptibility to colorectal cancer(CRC).However,the shared underlying mechanisms between UC and CRC remain unclear.Given the therapeutic potential of luteolin in both UC and CRC,this study aims to elucidate the molecular targets and mechanisms through which luteolin exerts its effects against these diseases.Methods:The GeneCards database,DisGENet database,and Gene Expression Omnibus database were utilized to analyze the targets associated with UC and CRC.Subsequently,the Traditional Chinese Medicine Systems Pharmacology and SwissTargetPrediction databases were employed to identify luteolin-related targets.The identified luteolin-related targets were then mapped to official gene symbols using the UniProt database.The Cytoscape 3.9.0 software was utilized to construct a network of luteolin-associated targets.Venn diagram analysis was performed to identify common targets among UC,CRC,and luteolin.The common targets were further analyzed using the STRING database to construct a protein-protein interaction network.The“cytoHubba”plugin in Cytoscape 3.9.0 was employed to identify hub targets within the PPI network.Gene Ontology functional analysis and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis were conducted on the hub targets.Finally,molecular docking using AutoDock and PyMOL software was performed to assess the binding affinity between luteolin and the hub targets.Results:Luteolin was found to interact with a total of 149 pharmacological targets,while UC and CRC were associated with 1232 and 3278 targets,respectively.Forty-six common targets were identified among luteolin,UC,and CRC.Through the application of seven different algorithms,seven hub targets were identified,TP53,AKT1,TNF,SRC,EGFR,and MMP9.Bioinformatics enrichment analysis revealed 49 enriched pathways through Kyoto Encyclopedia of Genes and Genomes analysis,while Gene Ontology analysis yielded a total of 245 biological processes,4 cellular components,and 7 molecular functions.Molecular docking simulations demonstrated a good binding affinity between luteolin and the hub targets.Conclusion:This study identified multiple potential pharmacological targets and elucidated various biological pathways through which luteolin may exert its therapeutic effects in the treatment of UC and CRC.These findings provide a solid theoretical foundation for further experimental investigations in the treatment of UC and CRC.
文摘Objective:To obtain Iuteolin and apigenin rich fraction from the ethanolic extract of Cynodon dactylon(L.)(C.dactylon) Pers and evaluate the fraction's cytotoxicity and anti-Chikungunya potential using Vero cells.Methods:The ethanolic extract of C.dactylon was subjected to silica gel column chromatography to obtain anti-chikungunya virus(CHIKV) fraction.Reverse phase-HPLC and GC-MS studies were carried out to identily the major phytochemicals in the fraction using phylochemical standards.Cytotoxicity and the potential of the fraction against CHIKV were evaluated in vitro using Vero cells.Reduction in viral replication was assessed by reverse transcriptase-polymerase chain reaction(RT-PCR) after treating the viral infected Vero cells with the fraction.Results:Reverse Phase-HPLC and GC-MS studies confirmed the presence of flavonoids,luteolin and apigenin as major phytochemicals in the anti-CHIKV ethanolic fraction of C.dactylon- The fraction was found to exhibit potent viral inhibitory activity(about 98%) at the concentration of 50 μg/mL as observed by reduction in cytopathic effect,and the cytotoxic concentration of the fraction was found to be 250 μg/mL.RT-PCR analyses indicated that the reduction in viral mRNA synthesis in fraction treated infected cells was much higher than the viral infected control cells.Conclusions:Luteolin and apigenin rich ethanolic fraction from C.dactylon can be utilized as a potential therapeutic agent against CHIKV infection as the fraction does not show cytotoxicity while inhibiting the virus.
基金supported by the Project Funding for the Training of High Level Health Professionals in Changzhou of China,No.2016CZLJ013(to BY)Science and Technology Support Project of Changzhou of China,No.Social Development CE20175021(to BY)Application Basic Research Project of Changzhou of China,No.CJ20160036(to WBZ)
文摘Studies have shown that the natural flavonoid luteolin has neurotrophic activity. In this study, we investigated the effect of luteolin in a mouse model of Down syndrome. Ts65 Dn mice, which are frequently used as a model of Down syndrome, were intraperitoneally injected with 10 mg/kg luteolin for 4 consecutive weeks starting at 12 weeks of age. The Morris water maze test was used to evaluate learning and memory abilities, and the novel object recognition test was used to assess recognition memory. Immunohistochemistry was performed for the neural stem cell marker nestin, the astrocyte marker glial fibrillary acidic protein, the immature neuron marker DCX, the mature neuron marker NeuN, and the cell proliferation marker Ki67 in the hippocampal dentate gyrus. Nissl staining was used to observe changes in morphology and to quantify cells in the dentate gyrus. Western blot assay was used to analyze the protein levels of brain-derived neurotrophic factor(BDNF) and phospho-extracellular signal-regulated kinase 1/2(p-ERK1/2) in the hippocampus. Luteolin improved learning and memory abilities as well as novel object recognition ability, and enhanced the proliferation of neurons in the hippocampal dentate gyrus. Furthermore, luteolin increased expression of nestin and glial fibrillary acidic protein, increased the number of DCX^+ neurons in the granular layer and NeuN^+ neurons in the subgranular region of the dentate gyrus, and increased the protein levels of BDNF and p-ERK1/2 in the hippocampus. Our findings show that luteolin improves behavioral performance and promotes hippocampal neurogenesis in Ts65 Dn mice. Moreover, these effects might be associated with the activation of the BDNF/ERK1/2 pathway.
基金This work was supported by the Zhejiang Provincial Ten Thousand Program for Leading Talents of Science and Technology Innovation[2018R52050]the National Natural Science Fund of China[Grant numbers:82073963,81522049,81703636,31571735,31270007]+1 种基金the Zhejiang Provincial Program for the Cultivation of High-Level Innovative Health Talents,the Research Project of Zhejiang Chinese Medical University(2021JKZDZC06)the Opening Project of Zhejiang Provincial Preponderant and Characteristic Subject of Key University(Traditional Chinese Pharmacology),Zhejiang Chinese Medical University[ZYAOXZD2019006]。
文摘Polyphenols are the main active components of the anti-inflammatory compounds in dandelion,and chlorogenic acid(CGA)is one of the primary polyphenols.However,the molecular mechanism underlying the transcriptional regulation of CGA biosynthesis remains unclear.Hydroxycinnamoyl-CoA:quinate hydroxycinnamoyl transferase(HQT2)is the last rate-limiting enzyme in chlorogenic acid biosynthesis in Taraxacum antungense.Therefore,using the TaHQT2 gene promoter as a probe,a yeast one-hybrid library was performed,and a basic helix-loop-helix(bHLH)transcription factor,TabHLH1,was identified that shared substantial homology with Gynura bicolor DC bHLH1.The TabHLH1 transcript was highly induced by salt stress,and the TabHLH1 protein was localized in the nucleus.CGA and luteolin concentrations in TabHLH1-overexpression transgenic lines were significantly higher than those in the wild type,while CGA and luteolin concentrations in TabHLH1-RNA interference(RNAi)transgenic lines were significantly lower.Quantitative real-time polymerase chain reaction demonstrated that overexpression and RNAi of TabHLH1 in T.antungense significantly affected CGA and luteolin concentrations by upregulating or downregulating CGA and luteolin biosynthesis pathway genes,especially TaHQT2,4-coumarate-CoA ligase(Ta4CL),chalcone isomerase(TaCHI),and flavonoid-3′-hydroxylase(TaF3′H).Dual-luciferase,yeast one-hybrid,and electrophoretic mobility shift assays indicated that TabHLH1 directly bound to the bHLH-binding motifs of proTaHQT2 and proTa4CL.This study suggests that TabHLH1 participates in the regulatory network of CGA and luteolin biosynthesis in T.antungense and might be useful for metabolic engineering to promote plant polyphenol biosynthesis.
基金Thanks for the support of the National Natural Science Foundation of China(https://isisn.nsfc.gov.cn/egrantindex/funcindex/prjsearch-list,grant nos.:81673147,81372985)he Fundamental Research Funds for the Central Universities(grant no.:2242017K40035)Postgraduate Research&Practice Innovation Program of Jiangsu Province(grant no.:SJCX170070).
文摘The present study was conducted to verify the influence of luteolin on apoptosis of Eca109 cells and to further investigate the possible mechanisms underlying its effect on apoptosis.The cells were exposed to different concentrations of luteolin(0,40,80,120,160,200,240M)for 24,48,and 72 h respectively.The influence of luteolin on proliferation of Eca109 cells was detected using MTT assay.Eca109 cells were then treated with luteolin(0,40,160,240M)for 24 h.The effect of luteolin on cell cycle progression and apoptosis was assayed by using flow cytometry(FCM).Expression of caspase9 and caspase3 mRNA and protein was analyzed by real-time PCR and Western blot respectively.The results showed that luteolin could inhibit the proliferation of Eca109 cells at all concentrations in a time-dependent manner and the relative inhibition rate showed an inverted U-shaped association with the concentration of luteolin.Further,the cell cycle was arrested in the S phase following treatment with luteolin.Apoptosis analysis indicated that luteolin could induce the apoptosis of Eca109 cells across the three concentration groups,which exhibited a trend of first promotional and then inhibitory with the increases in luteolin concentration.The effect of luteolin on the mRNA and protein expression of caspase 9 and caspase3 first manifested as promotion,then inhibition.Therefore,luteolin may serve a role in promoting cell apoptosis by inducing Eca109 cell apoptosis that involves the expression of caspase3,caspase9 mRNA and protein.This study provides theoretical basis for further study and clinical application of luteolin.The specific mechanism has not yet been clarified and the other activation pathways inducing apoptosis need to be further studied.
文摘AIM:To investigate the effects of luteolin on apoptosis,the cell cycle,and the expression and secretion of vascular endothelial growth factor(VEGF)in human choroidal melanoma cells(C918 and OCM-1).METHODS:C918 and OCM-1 cells cultured in vitro were treated with various concentrations of luteolin(0,5,10,15μmol/L).Cell growth was observed with an inverted microscope,and cell cycle arrest was detected by propidium iodide(PI)staining using flow cytometry.Apoptosis was detected by Hoechst33342 staining,and apoptosis rate was determined by Annexin V-FITC/PI experiments using flow cytometry.The expression of apoptosis-related proteins Bcl-2,Bax and VEGF was analyzed using Western blots.The levels of VEGF secreted by the cells into the supernatant was analyzed using ELISA.RESULTS:After treating with 5 to 15μmol/L luteolin for 48 h,the fusion degree of C918 and OCM-1 cells decreased,and more floating apoptotic cells appeared.Luteolin treatment increased the G0-G1 phase ratio of the C918 and OCM-1 cells,blocked cell cycle progression,and increased the apoptosis rate of the C918 and OCM-1 cells.Western blot showed that luteolin decreased the expression of Bcl-2 and VEGF in the C918 and OCM-1 cells and increased the expression of Bax protein.The ELISA results showed that 10 to 15μmol/L luteolin decreased the cell secretion of VEGF.CONCLUSION:Luteolin may induce apoptosis by regulating the levels of apoptosis-related proteins in C918 and OCM-1 cells.Luteolin can induce cell cycle arrest,decrease the expression of VEGF.
基金The work was supported by the National Natural Science Foundation of China,Nos.81470656(to YX),82071372(to AL),82074169(to XSM)Guangdong Grant Key Technologies for Treatment of Brain Disorders’,China,No.2018B030332001(to YX)+3 种基金Ningxia Key Research and Development Program Grant(Yinchuan,Ningxia Hui Autonomous Region,China)(to KFS)Program of Introducing Talents of Discipline to Universities,China,No.B14036(to YX,AL,KFS)Outstanding Scholar Program of Bioland Laboratory(Guangzhou Regenerative Medicine and Health Guangdong Laboratory),No.2018GZR110102002(to KFS,AL)Science and Technology Program of Guangzhou,No.202007030012(to KFS and AL).
文摘Luteolin is neuroprotective for retinal ganglion cells and retinal pigment epithelial cells after oxidative injury,whereby it can inhibit microglial neurotoxicity.Therefore,luteolin holds the potential to be useful for treatment of retinal diseases.The purpose of this study was to investigate whether luteolin exhibits neuroprotective effects on rod cells in rd10 mice,a slow photoreceptor-degenerative model of retinitis pigmentosa.Luteolin(100 mg/kg)intraperitoneally injected daily from postnatal day 14(P14)to P25 significantly enhanced the visual performance and retinal light responses of rd10 mice at P25.Moreover,it increased the survival of photoreceptors and improved retinal structure.Mechanistically,luteolin treatment attenuated increases in reactive oxygen species,photoreceptor apoptosis,and reactive gliosis;increased mRNA levels of anti-inflammatory cytokines while lowering that of pro-inflammatory and chemoattractant cytokines;and lowered the ratio of phospho-JNK/JNK.Application of the JNK inhibitor SP600125 exerted a similar protective effect to luteolin,suggesting that luteolin delays photoreceptor degeneration and functional deterioration in rd10 mice through regulation of retinal oxidation and inflammation by inhibiting the JNK pathway.Therefore,luteolin may be useful as a supplementary treatment for retinitis pigmentosa.This study was approved by the Qualified Ethics Committee of Jinan University,China(approval No.IACUC-20181217-02)on December 17,2018.
文摘Natural dyestuff of luteolin was isolated and used to dye wool fabric in this paper. Ethanol extraction and high-speed countercurrent chromatography (HSCCC) were used to extract and purify the luteolin from the peanut shell, and the structure of the isolated luteolin was characterized with FTIR techniques. The interaction between dyestuff and fiber was preliminarily discussed through thermodynamic study and supramolecular structure simulation to explain the intrinsic reasons why the color fastness was low when luteolin was applied to dyeing wool fabric. The extraction condition and purification parameter were as follows: 65% ethanol, ratio of material to liquid 1:20, 80°C, 3 h, chloroform-methanol-water (4/3/2, V/V), 800 rmp/min, 2.0 Mkpa, 0.5 mL/ min and 280 nm. The results of dyeing thermodynamics showed that the sorption isotherm of luteolin on wool fabric was consistent with Nernst model and similar to the disperse dyestuff. With molecular simulation, luteolin and glycin composed 8 stable complexes whose Laplacian values all were greater than 0, which suggested typical hydrogen bonds existing. The complex with three hydrogen bonds was proved the most stable. Both studies on thermodynamics and supramolecular simulation revealed that luteolin on wool fabric mainly depended on the weak hydrogen bonds interaction that determined the low dyefastness.
文摘Studies have indicated that flavonoid luteolin is a potential inhibitor of tumor cell proliferation and may function as an anticarcinogenic agent. Pyrrolidine dithiocarbamate (PDTC), a synthetic compound, may exhibit biphasic effects on apoptosis depending on the experimental context. Previously, we found that luteolin induced the activation of the proapoptotic proteins, such as Bad, Bid, and Bax, in HL-60 human leukemia cells. We also explored the modulatory effects and molecular mechanisms of PDTC on the cytotoxicity of luteolin in HL-60 cells;PDTC could interfere with luteolin’s ability to cleave poly(ADP-ribose)-polymerase (PARP) and DNA fragmentation of factor-45 (DFF-45). In the current study, we further investigated the effect of PDTC on the luteolin-induced death-receptor pathway and the cleavage of the Bcl-2 family members. We found that the combination of luteolin and PDTC increased the survival of the HL-60 cells such that PDTC inhibited both extrinsic and intrinsic pathways in luteolin-induced apoptosis.
文摘Objective This study aimed to investigate the effect of exposure to a 900 MHz electromagnetic field(EMF)on the cervical spinal cord(CSC)of rats and the possible protective effect of luteolin(LUT)against CSC tissue damage.Methods Quantitative data were obtained via stereological,biochemical,immunohistochemical,and histopathological techniques.We investigated morphometric value,superoxide dismutase(SOD)level,and the expression of high-mobility group box 1 protein molecules,as well as histological changes.Results The total number of motor neurons in the EMF group significantly decreased in comparison with that in the control group(P<0.05).In the EMF+LUT group,we found a significant increase in the total number of motor neurons compared with that in the EMF group(P<0.05).SOD enzyme activity in the EMF group significantly increased in comparison with that in the control group(P<0.05).By contrast,the EMF+LUT group exhibited a decrease in SOD level compared with the EMF group(P<0.05).Conclusion Our results suggested that exposure to EMF could be deleterious to CSC tissues.Furthermore,the protective efficacy of LUT against SC damage might have resulted from the alleviation of oxidative stress caused by EMF.
基金Supported by 2019 Undergraduate Innovation and Enterpreneurship Training Program of Fujian Province(201912631026)Undergraduate Innovation and Enterpreneurship Training Program of Xiamen Medical College(201912631057)。
文摘[Objectives] This study was conducted to optimize the extraction process of luteolin, a natural product of Ternate Grape Fern Herb, so as to provide a theoretical research basis for the chemical composition, pharmacological research and preparation development of Ternate Grape Fern Herb. [Methods] The effects of four factors including ultrasonic extraction time, liquid-to-material ratio, ultrasonic extraction temperature and ethanol concentration on extraction yield of luteolin from Ternate Grape Fern Herb were investigated by the ultrasonic-assisted extraction technology, through a single-factor experiment and an orthogonal experiment based on the single-factor experiment, and the experimental data was subjected to variance analysis and SSR analysis of the significance of differences between groups by SPSS20.0 software to select the optimal extraction process conditions. [Results] The optimal extraction process conditions were as follows: ultrasonic extraction time 25 min, liquid-to-material ratio 25∶1 ml/g, ethanol volume fraction 60%, ultrasonic extraction temperature 85 ℃. [Conclusions] The results were verified, and it was determined that the ultrasonic-assisted extraction process established in this study is reasonable and reliable, and can be used for the extraction of luteolin.
基金Supported by Independent Research Project of Key Laboratory of Extraction,Purification and Quality Analysis of Traditional Chinese Medicine in Guangxi Universities(J1606701).
文摘[Objectives]To determine the content of hyperin and luteolin in Vernonia cinerea(L.)Less.,and to provide basic experimental data for experimental research and clinical application of V.cinerea(L.)Less.[Methods]The components to be determined were extracted by ultrasonic extraction,and the hyperin and luteolin in V.cinerea(L.)Less.were separated and determined by reversed-phase high performance liquid chromatography(chromatographic conditions:reverse C 18 column,methanol-0.4%phosphoric acid as mobile phase for gradient elution,flow rate of 1.0 mL/min,detector wavelength of 360 nm).[Results]The precision and repeatability of the experimental method were good,and the solution of V.cinerea(L.)Less.was stable within 18 h.There was a good linear relationship between the injection volume of hyperin and luteolin and the chromatographic peak area within the prescribed range,and the recovery rate was qualified.The content of hyperin and luteolin in V.cinerea(L.)Less.was 0.0665 mg/g(n=3)and 0.1004 mg/g(n=3),respectively.[Conclusions]The experimental method is sensitive,specific,stable,accurate and reliable,and can be used for the determination of hyperin and luteolin in V.cinerea(L.)Less.
基金supported by Hainan Health and Family Planning Industry Scientific Research Project(Grant No.18A200018).
文摘Objective: To explore the anti-inflammatory effect of luteolin on rats with acute gout arthritis. Methods: A total of forty-eight rats were chosen and randomly divided into six groups. The acute gout arthritis model of rats was established by injecting monosodium urate at the concentration of 25 mg/mL into the ankle joint cavity. Changes of joint swelling index at different time points and the levels of IL-1β, IL-17, TNF-α, IL-6 in serum and synovial were measured. Results: Compared with the control group, the swelling index of ankle joint and gait score in the model group was significantly enhanced at different time points (all P<0.01). Compared with the MSU group, luteolin improved the ankle swelling index of rats with acute gout arthritis (P<0.05), and significantly down-regulated the levels of IL-1β, IL-17, TNF-α, IL-6 (P<0.01). Conclusion: Luteolin can alleviate the inflammatory response of acute gouty arthritis and may be an effective drug for acute gouty arthritis.
