[Objective] The aim was to provide scientific basis for breeding on the fruit width of summer squash.[Method] Inheritance of the fruit width traits of summer squash in P1,P2,F1,BC1,BC2 and F2 from two crosses q-1×...[Objective] The aim was to provide scientific basis for breeding on the fruit width of summer squash.[Method] Inheritance of the fruit width traits of summer squash in P1,P2,F1,BC1,BC2 and F2 from two crosses q-1×23-4G（cross 1）and q-1×A-7（cross 2）was investigated by the mixed major gene and polygene inheritance model of quantitative traits.[Result] The fruit width was controlled by B-1 model.Heritability values of the major genes of F2 were relatively high and the environmental effect was relatively low.[Conclusion] The fruit width breeding of summer squash should be selected in early generation selection.展开更多
[Objective]The aim was to provide scientific basis for breeding on the fruit length of summer squash.[Method]Inheritance of the fruit length of summer squash in P1,P2,F1,B1,B2 and F2 from two crosses q-1 ×23-4G（...[Objective]The aim was to provide scientific basis for breeding on the fruit length of summer squash.[Method]Inheritance of the fruit length of summer squash in P1,P2,F1,B1,B2 and F2 from two crosses q-1 ×23-4G（cross 1） and q-1 ×A-7（cross 2） was investigated by the mixed major gene and polygene inheritance model of quantitative traits.[Result]The results showed that the fruit length was controlled by D2 model;Genetic effect of fruit length of cross 1 was mainly additive effect,but genetic effect of fruit length of cross 2 was mainly dominant effect.Heritability values of the genes of F2 were relatively high and the environmental effect was relatively low.[Conclusion]The fruit length breeding of summer squash should be selected in early generation selection.展开更多
Segregation analysis of the mixed genetic model of major gene plus polygene was used to identify the major genes for cotton yield-related traits using six generations P1, P2, F1, B1, B2, and F2 generated from the cros...Segregation analysis of the mixed genetic model of major gene plus polygene was used to identify the major genes for cotton yield-related traits using six generations P1, P2, F1, B1, B2, and F2 generated from the cross of Baimian 1 x TM-1. In addition to boll size and seed index, the major genes for the other five traits were detected： one each for seed yield, lint percentage, boll number, lint index; and two for lint yield. Quantitative trait locus/loci （QTL） mapping was performed in the F2 and F2：3 populations of above cross through molecular marker technology, and a total of 50 QTL （26 suggestive and 24 significant） for yield-related traits were detected. Four common QTL were discovered： qLP-3b（F2）/qLP-3（F2：3） and qLP-19b （F2）/qLP-19（F2：3） for lint percentage, qBN-17（F2）/qBN-17（F2：3） for boll number, and qBS-26b（F2）/qBS-26（F2：3） for boll size. Especially, qLP- 3b（Fz）/qLP-3（F2：3）, not only had LOD scores 〉3 but also exceeded the permutation threshold （5.13 and 5.29, respectively）, correspondingly explaining 23.47 and 29.55% of phenotypic variation. This QTL should be considered preferentially in marker assisted selection （MAS）. Segregation analysis and QTL mapping could mutually complement and verify, which provides a theoretical basis for genetic improvement of cotton yield-related traits by using major genes （QTL）.展开更多
The joint analysis of the mixed genetic model of major gene and polygene was conducted to study the inheritance of cryotolerance in cotton during the overwintering period.H077（G.hirsutum L.,weak cryotolerance） and H...The joint analysis of the mixed genetic model of major gene and polygene was conducted to study the inheritance of cryotolerance in cotton during the overwintering period.H077（G.hirsutum L.,weak cryotolerance） and H113（G.barbadence L.,strong cryotolerance） were used as parents.Cryotolerance of six generation populations including P1,P2,F1,B1,B2,and F2,from each of the two reciprocal crosses H077×H113 and H113×H077 were all investigated.The results showed that cryotolerance in cotton during the overwintering period was accorded with two additive major genes and additivedominance polygene genetic model.For cross H077×H113,the heritabilities of major genes in B1,B2,and F2 were 83.62,76.84,and 90.56%,respectively;and the heritability of polygene could only be detected in B2,which was 7.76%.For cross H113×H077,the heritabilities of major genes in B1,B2,and F2 were 67.42,68.95,and 83.40%,respectively;and the heritability of polygene was only detected in F2,which was 6.51%.In addition,the whole heritability in F2 was always higher than that in B1 and B2 in each cross.Therefore,for the cryotolerance breeding of perennial cotton,the method of single cross recombination or single backcross should be adopted to transfer major genes,and the selection in F2 would be more efficient than that in other generations.展开更多
Based on the major gene and polygene mixed inheritance model for multiple correlated quantitative traits, the authors proposed a new joint segregation analysis method of major gene controlling multiple correlated quan...Based on the major gene and polygene mixed inheritance model for multiple correlated quantitative traits, the authors proposed a new joint segregation analysis method of major gene controlling multiple correlated quantitative traits, which include major gene detection and its effect and variation estimation. The effect and variation of major gene are estimated by the maximum likelihood method implemented via expectation-maximization （EM） algorithm. Major gene is tested with the likelihood ratio （LR） test statistic. Extensive simulation studies showed that joint analysis not only increases the statistical power of major gene detection but also improves the precision and accuracy of major gene effect estimates. An example of the plant height and the number of tiller of F2 population in rice cross Duonieai x Zhonghua 11 was used in the illustration. The results indicated that the genetic difference of these two traits in this cross refers to only one pleiotropic major gene. The additive effect and dominance effect of the major gene are estimated as -21.3 and 40.6 cm on plant height, and 22.7 and -25.3 on number of tiller, respectively. The major gene shows overdominance for plant height and close to complete dominance for number of tillers.展开更多
The inheritance of stripe disease resistance in a rice restorer line C224 was analyzed using the mixed effect model of major gene plus polygene for quantitative traits.In addition,the resistance was investigated in se...The inheritance of stripe disease resistance in a rice restorer line C224 was analyzed using the mixed effect model of major gene plus polygene for quantitative traits.In addition,the resistance was investigated in seven crosses of C224 with maintainer lines.The results showed that the stripe resistance of C224 was controlled by two major genes with additive-dominance-epistasis effects plus polygenes with additive-dominance effects （E-1 model）.These two genes had additive effects of-12.47% and-24.75%,respectively,showing negative dominance effects.There were significant epistasis and interaction effects between the two major genes.The heritability of the two major genes was 92.12%,while that of polygenes was 2.74%,indicating that the stripe resistance had dominant major gene effect.Of the seven crosses,five displayed high or medium resistance to the stripe disease.展开更多
[ Objective ] This study aimed to analyze the inheritance of bolting associated traits in Brassica rapa, which will provide useful information in a breeding program for late-bolting or bolting-resistant cultivars of C...[ Objective ] This study aimed to analyze the inheritance of bolting associated traits in Brassica rapa, which will provide useful information in a breeding program for late-bolting or bolting-resistant cultivars of Chinese cabbage. [ Method] Three phenotypic measurements, bolting index, flowering time, days to 5 cm elongated stalk, respectively were used for inheritance analysis of six generations, P, （bolting resistant inbreed line ）, P2 （vernalization independent type） and their filial generations F1 , B1, B2 and F2, using the mixed major-gene plus polygene inheritance model. [ Result] The two traits, bolting index and days to 5 cm elongated stalk, both were controlled by two major genes with additive-dominant-epistatic effects （ B-1 model） in hybrid. The flowering time was controlled by one major gene with addltive-dominant effects plus additive-dominant-epistatic effects （D model）. The heritability of the major genes in B1, B2 and F2 were 96.22%, 93.33%, 93.55% for bolting index, 70.68%, 70.68%, 70.64% for flowering time, 79.44%, 79.55%, 79.38% for days to 5-cm elongated stalk, respectively, but no polygene heritability was detected in BI, B2 and F2 generation. It indicated that the bolting trait in Brassica rapa was controlled by one or tow major genes. [ Conclusion] This implied that in the genetic improvement for bolting resistant trait major gene was a main factor. It is fit for early selection and environment factor should be mentioned.展开更多
The existence of major genes affecting fecundity in sheep flocks throughout the world has been demonstrated.Three major genes whose mutations can increase ovulation rate have been discovered,and all related to the tra...The existence of major genes affecting fecundity in sheep flocks throughout the world has been demonstrated.Three major genes whose mutations can increase ovulation rate have been discovered,and all related to the transforming growth factorβ(TGF-β)superfamily.The mutant FecB of bone morphogenetic protein receptor 1B(BMPR1B)has an additive effect on ovulation rate.Six mutations(Fec^(XI),Fec^(XH),Fec^(XG),Fec^(XB),Fec^(XL),Fec^(XR))of bone morphogenetic protein 15(BMP15)related with fertility have been identified that share the same mechanism.All the mutants can increase ovulation rate in heterozygotes and cause complete sterility in homozygotes.Homozygous ewes with two new mutations(FecX^(Gr),FecX^(O))of BMP15 had increased ovulation rate without causing sterility.There are five mutations in growth differentiation factor 9(GDF9)associated with sheep prolificacy where FecG^(E) and FecG^(F) have additive an effect on ovulation rate and litter size.The newly identifiedβ-1,4-N-acetylgalactosaminyltransferase 2(B4GALNT2)gene of FecL is proposed as a new mechanism of ovulation rate regulation in sheep.Woodlands is an X-linked maternally imprinted gene which increases ovulation rate.In addition,several putative major genes need to be verified.This review is focused on the identification of the mutations and mechanisms whereby the major genes affecting ovulation rate.展开更多
Inheritance of line Jinghe891-l resistant to pathotype of Puccinia striiformis in two patterns of temperature (Normal: day 18℃ /night 10℃ , High: day 24℃ /night 15℃ )was studied in this paper. The results showed t...Inheritance of line Jinghe891-l resistant to pathotype of Puccinia striiformis in two patterns of temperature (Normal: day 18℃ /night 10℃ , High: day 24℃ /night 15℃ )was studied in this paper. The results showed that there were at least two pairs of dominant major genes and one pair of recessive minor genes in Jinghe 891-1. The two pairs of major genes that conferred resistance to CY31 were allelic or linked closely with resistance gene in Jubilejna Ⅱ , Kangyin655 and T. spelta Album. They were novel resistance genes and were inherited in a repeated or independent mode. The minor genes, which could modify the major genes, were sensitive to temperature and conferred resistance to all pathotypes of Puccinia striiformis in China. It is recommended that this line can be used as an important resource stock.展开更多
An insertion fragment in porcine FSHβ subunit gene was cloned by PCR. Sequencing data show that the insertion is a retroposon of 292 bp siting in intronⅠ at the site between +809 and +810 base. Based on these result...An insertion fragment in porcine FSHβ subunit gene was cloned by PCR. Sequencing data show that the insertion is a retroposon of 292 bp siting in intronⅠ at the site between +809 and +810 base. Based on these results, a PCR programme was created to genotype animal individuals in different pig breeds at FSHβ locus and polymorphism of FSHβ gene was analyzed. With the combination of genotype and litter size of sows, it was demonstrated that FSHβ locus is closely associated with major gene controlling litter size in commercial pig breeds, such as Yorkshire, Landrace, Durco. Averagely the AA sows give more 1.5 piglets than BB sows do per litter.展开更多
Previous studies have demonstrated that a missense single-nucleotide polymorphism variant （2316A〉G;rs2230739）of the adenylate cyclase type IX gene was associated with bipolar disorder and affective disorder.We dete...Previous studies have demonstrated that a missense single-nucleotide polymorphism variant （2316A〉G;rs2230739）of the adenylate cyclase type IX gene was associated with bipolar disorder and affective disorder.We determined genotype and allele frequencies using a ligase detection reaction method in 315 patients with major depressive disorder and 278 unrelated, sex-matched healthy control subjects.We did not detect any statistically significant differences in genotype and allele frequencies between patients and healthy control subjects.Furthermore,we found no significant difference between genders in major depressive disorder,nor between patients and controls in the same gender.These results suggest that 2316A〉G（rs2230739）may not be a risk factor for increasing susceptibility to major depressive disorder in the Chinese Han population.展开更多
[ Objective] This study aimed to detect flee blast resistance genes in major varieties of rice in Jilin Province. [ Method ] Ten molecular markers closely linked with rice blast resistance genes were used for molecula...[ Objective] This study aimed to detect flee blast resistance genes in major varieties of rice in Jilin Province. [ Method ] Ten molecular markers closely linked with rice blast resistance genes were used for molecular identification of resistance genes in 24 rice varieties from Jilin Province. [ Result] The 24 major vari- eties of rice widely lacked in rice blast resistance genes Pib, Pil, Pikh and Pia; Pi9 gene was absent in 17 of the 24 major varieties, especially in Jijing 88 and Changbai 9 which occupied the largest cultivation area in Jilin Province ; in addition, Jijing 88 harbored five rice blast resistance genes, including Pita, Pikm, Pt2, P/-d2 and P/z. [ Conclusion] Pib, Pil, Pikh, Pia and Pi9 are the major resistance genes for improving flee blast resistance of major varieties of rice in Jilin Prov- ince.展开更多
Objective To investigate the frequency of t(14; 18) in different subtypes of B-cell lymphomas and the ability or the polymerase chain reaction(PCR) to detect this rearrangement in frozen samples. Methods 1o7 cases of ...Objective To investigate the frequency of t(14; 18) in different subtypes of B-cell lymphomas and the ability or the polymerase chain reaction(PCR) to detect this rearrangement in frozen samples. Methods 1o7 cases of B-cell lymphomas were studied uslng DNA extracted from rresh-frozen tissues. The DNA samples were amplified by PCR for bcl-2 MBR/JH. The products of bcl-2/JH rearrangement were hybridized with an internal olignucleotide probe or bcl-2 MBR. Results The rearranged bcl-2MBR/JH gene was detected in 13 of the 25(52. o% ) follicular center lymphomas, according to REAL classification: 8 of 11 (72. 7%) grade 1, 2 of 5(40. 0%) grade I, and 3 of 90 (33. 3%) grade, 17 of 82(2o. 8%) cases or difruse large B-cell lymphomas were found to have detectable bel-2 MBR/J. rearrangement- Conclusion The rrequency or bcl-2 MBR/JH rearrangement in diffuse large B-cell lymphomas is significantly lower than those in follicular center lympkomas(X2= 9. 28, P <o. oo5), suggesting that bcl2/JH rearrangements occur mainly in follicular center lymphomas. in addition, the result of reconstruction experiments suggest that amplification or bcl-2 MBR/JH rearrangements by PCR is both sensitive and specific for detection of t (14; 18 ) translocation.展开更多
The norepinephrine transporter plays an important role in the pathophysiology and pharmacological treatment of major depressive disorder. Consequently, the norepinephrine transporter gene is an attractive candidate in...The norepinephrine transporter plays an important role in the pathophysiology and pharmacological treatment of major depressive disorder. Consequently, the norepinephrine transporter gene is an attractive candidate in major depressive disorder research. In the present study, we evaluated the depression symptoms of subjects with major depressive disorder, who were all from the North of China and of Hart Chinese origin, using the Hamilton Depression Scale. We examined the relationship between two single nucleotide polymorphisms in the norepinephrine transporter, rs2242446 and rs5569, and the retardation symptoms of major depressive disorder using quantitative trait testing with the UNPHASED program, rs5569 was associated with depressed mood, and the GG genotype may be a risk factor for this; rs2242446 was associated with work and interest, and the TT genotype may be a risk factor for loss of interest. Our findings suggest that rs2242446 and rs5569 in the norepinephrine transporter gene are associated with the retardation symptoms of depression in the Hart Chinese population.展开更多
According to the published gene sequence of the major surface antigen (P30) of Toxoplasma gondii, a pair of primers were designed and synthesized. Using polymerase chain reaction (PCR), the coding sequences of P30 gen...According to the published gene sequence of the major surface antigen (P30) of Toxoplasma gondii, a pair of primers were designed and synthesized. Using polymerase chain reaction (PCR), the coding sequences of P30 gene were amplified from a Chinese strain of T. gondii, The amplified gene fragment and plasmid pB220 were digested with EcoRI and BamHI and then ligated. The inserted gene fragment was sequenced by the chain termination method, the reading reveals that nucleotide sequence determined was the same as the P30 sequecne of RH strain pubilished by Burg (1988), except that one base was changed. The recombinant plasmid containing P30 gene was transformed to E. coli DH5α.After temperature inducing culture, the total cellular proteins were analysed by SDS-PAGE and Western blot. The results show that the p30 gene cloned into the plasmid could express in E. coli, and the expression product had immunogenicity.展开更多
A DH population derived from C49S-87/01Y1-1069 was used to study the inheritance of wheat haploid embryo production frequency（EPF） in wheat × maize cross with the mixed major gene and polygene inheritance model...A DH population derived from C49S-87/01Y1-1069 was used to study the inheritance of wheat haploid embryo production frequency（EPF） in wheat × maize cross with the mixed major gene and polygene inheritance model of quantitative traits. The results showed that the EPF of wheat × maize cross was controlled by two dominant epistatic genes and polygene with gene effects of 1.95 for the first major gene, 6.69 for the second one and 2.80 for the polygene. The inheritability of major genes was as high as 72.09%, suggesting that the differences in EPF among wheat materials were mainly influenced by genotype. However, non-genetic factors were still important, especially for wheat materials with low EPF.展开更多
基金Supported by Science and Technology Development Project of Jinan in 2006(064034)~~
文摘[Objective] The aim was to provide scientific basis for breeding on the fruit width of summer squash.[Method] Inheritance of the fruit width traits of summer squash in P1,P2,F1,BC1,BC2 and F2 from two crosses q-1×23-4G（cross 1）and q-1×A-7（cross 2）was investigated by the mixed major gene and polygene inheritance model of quantitative traits.[Result] The fruit width was controlled by B-1 model.Heritability values of the major genes of F2 were relatively high and the environmental effect was relatively low.[Conclusion] The fruit width breeding of summer squash should be selected in early generation selection.
基金Supported by Science and Technology Development Project of Jinan City in2006(064034)~~
文摘[Objective]The aim was to provide scientific basis for breeding on the fruit length of summer squash.[Method]Inheritance of the fruit length of summer squash in P1,P2,F1,B1,B2 and F2 from two crosses q-1 ×23-4G（cross 1） and q-1 ×A-7（cross 2） was investigated by the mixed major gene and polygene inheritance model of quantitative traits.[Result]The results showed that the fruit length was controlled by D2 model;Genetic effect of fruit length of cross 1 was mainly additive effect,but genetic effect of fruit length of cross 2 was mainly dominant effect.Heritability values of the genes of F2 were relatively high and the environmental effect was relatively low.[Conclusion]The fruit length breeding of summer squash should be selected in early generation selection.
基金supported by the National Natural Science Foundation of China(31371677)the High-Tech R&D Program of China(2012AA101108)+2 种基金the Achievements Transformation Project of National Agricultural Science and Technology,China(2010276)the Research and Establishment of Modern Industrial Technology System for National Cotton,China(nycytx-06-09)the Natural Science Foundation of Henan Province,China(2010A210006)
文摘Segregation analysis of the mixed genetic model of major gene plus polygene was used to identify the major genes for cotton yield-related traits using six generations P1, P2, F1, B1, B2, and F2 generated from the cross of Baimian 1 x TM-1. In addition to boll size and seed index, the major genes for the other five traits were detected： one each for seed yield, lint percentage, boll number, lint index; and two for lint yield. Quantitative trait locus/loci （QTL） mapping was performed in the F2 and F2：3 populations of above cross through molecular marker technology, and a total of 50 QTL （26 suggestive and 24 significant） for yield-related traits were detected. Four common QTL were discovered： qLP-3b（F2）/qLP-3（F2：3） and qLP-19b （F2）/qLP-19（F2：3） for lint percentage, qBN-17（F2）/qBN-17（F2：3） for boll number, and qBS-26b（F2）/qBS-26（F2：3） for boll size. Especially, qLP- 3b（Fz）/qLP-3（F2：3）, not only had LOD scores 〉3 but also exceeded the permutation threshold （5.13 and 5.29, respectively）, correspondingly explaining 23.47 and 29.55% of phenotypic variation. This QTL should be considered preferentially in marker assisted selection （MAS）. Segregation analysis and QTL mapping could mutually complement and verify, which provides a theoretical basis for genetic improvement of cotton yield-related traits by using major genes （QTL）.
基金supported by the Innovation Project of Guangxi Postgraduate Education,China(2008105930901D015)
文摘The joint analysis of the mixed genetic model of major gene and polygene was conducted to study the inheritance of cryotolerance in cotton during the overwintering period.H077（G.hirsutum L.,weak cryotolerance） and H113（G.barbadence L.,strong cryotolerance） were used as parents.Cryotolerance of six generation populations including P1,P2,F1,B1,B2,and F2,from each of the two reciprocal crosses H077×H113 and H113×H077 were all investigated.The results showed that cryotolerance in cotton during the overwintering period was accorded with two additive major genes and additivedominance polygene genetic model.For cross H077×H113,the heritabilities of major genes in B1,B2,and F2 were 83.62,76.84,and 90.56%,respectively;and the heritability of polygene could only be detected in B2,which was 7.76%.For cross H113×H077,the heritabilities of major genes in B1,B2,and F2 were 67.42,68.95,and 83.40%,respectively;and the heritability of polygene was only detected in F2,which was 6.51%.In addition,the whole heritability in F2 was always higher than that in B1 and B2 in each cross.Therefore,for the cryotolerance breeding of perennial cotton,the method of single cross recombination or single backcross should be adopted to transfer major genes,and the selection in F2 would be more efficient than that in other generations.
基金This research was supported by the National Natural Science Foundation of China to Xu Chenwu （39900080, 30270724 and 30370758）.
文摘Based on the major gene and polygene mixed inheritance model for multiple correlated quantitative traits, the authors proposed a new joint segregation analysis method of major gene controlling multiple correlated quantitative traits, which include major gene detection and its effect and variation estimation. The effect and variation of major gene are estimated by the maximum likelihood method implemented via expectation-maximization （EM） algorithm. Major gene is tested with the likelihood ratio （LR） test statistic. Extensive simulation studies showed that joint analysis not only increases the statistical power of major gene detection but also improves the precision and accuracy of major gene effect estimates. An example of the plant height and the number of tiller of F2 population in rice cross Duonieai x Zhonghua 11 was used in the illustration. The results indicated that the genetic difference of these two traits in this cross refers to only one pleiotropic major gene. The additive effect and dominance effect of the major gene are estimated as -21.3 and 40.6 cm on plant height, and 22.7 and -25.3 on number of tiller, respectively. The major gene shows overdominance for plant height and close to complete dominance for number of tillers.
基金supported by the Guiding Plans for Natural Sciences Foundation of Liaoning Province,China(Grant No.20092207)the Special Foundation for Young Scientists of Liaoning Rice Research Institute,Shenyang,China(Grant No.DZS-2008-1)
文摘The inheritance of stripe disease resistance in a rice restorer line C224 was analyzed using the mixed effect model of major gene plus polygene for quantitative traits.In addition,the resistance was investigated in seven crosses of C224 with maintainer lines.The results showed that the stripe resistance of C224 was controlled by two major genes with additive-dominance-epistasis effects plus polygenes with additive-dominance effects （E-1 model）.These two genes had additive effects of-12.47% and-24.75%,respectively,showing negative dominance effects.There were significant epistasis and interaction effects between the two major genes.The heritability of the two major genes was 92.12%,while that of polygenes was 2.74%,indicating that the stripe resistance had dominant major gene effect.Of the seven crosses,five displayed high or medium resistance to the stripe disease.
基金Supported by the National Natural Science Foundation of China(30900981)the Scientific Research Fund for the Returned Overseas Chinese Scholars,Ministry Education of China(2010-1561)
文摘[ Objective ] This study aimed to analyze the inheritance of bolting associated traits in Brassica rapa, which will provide useful information in a breeding program for late-bolting or bolting-resistant cultivars of Chinese cabbage. [ Method] Three phenotypic measurements, bolting index, flowering time, days to 5 cm elongated stalk, respectively were used for inheritance analysis of six generations, P, （bolting resistant inbreed line ）, P2 （vernalization independent type） and their filial generations F1 , B1, B2 and F2, using the mixed major-gene plus polygene inheritance model. [ Result] The two traits, bolting index and days to 5 cm elongated stalk, both were controlled by two major genes with additive-dominant-epistatic effects （ B-1 model） in hybrid. The flowering time was controlled by one major gene with addltive-dominant effects plus additive-dominant-epistatic effects （D model）. The heritability of the major genes in B1, B2 and F2 were 96.22%, 93.33%, 93.55% for bolting index, 70.68%, 70.68%, 70.64% for flowering time, 79.44%, 79.55%, 79.38% for days to 5-cm elongated stalk, respectively, but no polygene heritability was detected in BI, B2 and F2 generation. It indicated that the bolting trait in Brassica rapa was controlled by one or tow major genes. [ Conclusion] This implied that in the genetic improvement for bolting resistant trait major gene was a main factor. It is fit for early selection and environment factor should be mentioned.
基金This work was funded by the Agricultural Science and Technology Innovation Program of China(ASTIP-IAS13)Key Technology Research and Development Program of Xinjiang Uygur Autonomous Region of China(200891102 and 2013911056)the Earmarked Fund for China Agriculture Research System(CARS-39).
文摘The existence of major genes affecting fecundity in sheep flocks throughout the world has been demonstrated.Three major genes whose mutations can increase ovulation rate have been discovered,and all related to the transforming growth factorβ(TGF-β)superfamily.The mutant FecB of bone morphogenetic protein receptor 1B(BMPR1B)has an additive effect on ovulation rate.Six mutations(Fec^(XI),Fec^(XH),Fec^(XG),Fec^(XB),Fec^(XL),Fec^(XR))of bone morphogenetic protein 15(BMP15)related with fertility have been identified that share the same mechanism.All the mutants can increase ovulation rate in heterozygotes and cause complete sterility in homozygotes.Homozygous ewes with two new mutations(FecX^(Gr),FecX^(O))of BMP15 had increased ovulation rate without causing sterility.There are five mutations in growth differentiation factor 9(GDF9)associated with sheep prolificacy where FecG^(E) and FecG^(F) have additive an effect on ovulation rate and litter size.The newly identifiedβ-1,4-N-acetylgalactosaminyltransferase 2(B4GALNT2)gene of FecL is proposed as a new mechanism of ovulation rate regulation in sheep.Woodlands is an X-linked maternally imprinted gene which increases ovulation rate.In addition,several putative major genes need to be verified.This review is focused on the identification of the mutations and mechanisms whereby the major genes affecting ovulation rate.
基金supported by the Beijing Natural Science Foundation(6962006).
文摘Inheritance of line Jinghe891-l resistant to pathotype of Puccinia striiformis in two patterns of temperature (Normal: day 18℃ /night 10℃ , High: day 24℃ /night 15℃ )was studied in this paper. The results showed that there were at least two pairs of dominant major genes and one pair of recessive minor genes in Jinghe 891-1. The two pairs of major genes that conferred resistance to CY31 were allelic or linked closely with resistance gene in Jubilejna Ⅱ , Kangyin655 and T. spelta Album. They were novel resistance genes and were inherited in a repeated or independent mode. The minor genes, which could modify the major genes, were sensitive to temperature and conferred resistance to all pathotypes of Puccinia striiformis in China. It is recommended that this line can be used as an important resource stock.
文摘An insertion fragment in porcine FSHβ subunit gene was cloned by PCR. Sequencing data show that the insertion is a retroposon of 292 bp siting in intronⅠ at the site between +809 and +810 base. Based on these results, a PCR programme was created to genotype animal individuals in different pig breeds at FSHβ locus and polymorphism of FSHβ gene was analyzed. With the combination of genotype and litter size of sows, it was demonstrated that FSHβ locus is closely associated with major gene controlling litter size in commercial pig breeds, such as Yorkshire, Landrace, Durco. Averagely the AA sows give more 1.5 piglets than BB sows do per litter.
基金funded by Natural Science Foundation of Department of Education of Henan Province,No.2009B20005
文摘Previous studies have demonstrated that a missense single-nucleotide polymorphism variant （2316A〉G;rs2230739）of the adenylate cyclase type IX gene was associated with bipolar disorder and affective disorder.We determined genotype and allele frequencies using a ligase detection reaction method in 315 patients with major depressive disorder and 278 unrelated, sex-matched healthy control subjects.We did not detect any statistically significant differences in genotype and allele frequencies between patients and healthy control subjects.Furthermore,we found no significant difference between genders in major depressive disorder,nor between patients and controls in the same gender.These results suggest that 2316A〉G（rs2230739）may not be a risk factor for increasing susceptibility to major depressive disorder in the Chinese Han population.
基金Supported by Breeding Program of Finance Department of Jilin Province(No.018)Achievement Transformation Project of Science and Technology Department of Jilin Province(No.20130305038NY)
文摘[ Objective] This study aimed to detect flee blast resistance genes in major varieties of rice in Jilin Province. [ Method ] Ten molecular markers closely linked with rice blast resistance genes were used for molecular identification of resistance genes in 24 rice varieties from Jilin Province. [ Result] The 24 major vari- eties of rice widely lacked in rice blast resistance genes Pib, Pil, Pikh and Pia; Pi9 gene was absent in 17 of the 24 major varieties, especially in Jijing 88 and Changbai 9 which occupied the largest cultivation area in Jilin Province ; in addition, Jijing 88 harbored five rice blast resistance genes, including Pita, Pikm, Pt2, P/-d2 and P/z. [ Conclusion] Pib, Pil, Pikh, Pia and Pi9 are the major resistance genes for improving flee blast resistance of major varieties of rice in Jilin Prov- ince.
文摘Objective To investigate the frequency of t(14; 18) in different subtypes of B-cell lymphomas and the ability or the polymerase chain reaction(PCR) to detect this rearrangement in frozen samples. Methods 1o7 cases of B-cell lymphomas were studied uslng DNA extracted from rresh-frozen tissues. The DNA samples were amplified by PCR for bcl-2 MBR/JH. The products of bcl-2/JH rearrangement were hybridized with an internal olignucleotide probe or bcl-2 MBR. Results The rearranged bcl-2MBR/JH gene was detected in 13 of the 25(52. o% ) follicular center lymphomas, according to REAL classification: 8 of 11 (72. 7%) grade 1, 2 of 5(40. 0%) grade I, and 3 of 90 (33. 3%) grade, 17 of 82(2o. 8%) cases or difruse large B-cell lymphomas were found to have detectable bel-2 MBR/J. rearrangement- Conclusion The rrequency or bcl-2 MBR/JH rearrangement in diffuse large B-cell lymphomas is significantly lower than those in follicular center lympkomas(X2= 9. 28, P <o. oo5), suggesting that bcl2/JH rearrangements occur mainly in follicular center lymphomas. in addition, the result of reconstruction experiments suggest that amplification or bcl-2 MBR/JH rearrangements by PCR is both sensitive and specific for detection of t (14; 18 ) translocation.
基金supported by the National Natural Science Foundation of China,No. 30770770,30971054,and81171290Science Foundation for the Youth of Shanxi,No.2011021036-1
文摘The norepinephrine transporter plays an important role in the pathophysiology and pharmacological treatment of major depressive disorder. Consequently, the norepinephrine transporter gene is an attractive candidate in major depressive disorder research. In the present study, we evaluated the depression symptoms of subjects with major depressive disorder, who were all from the North of China and of Hart Chinese origin, using the Hamilton Depression Scale. We examined the relationship between two single nucleotide polymorphisms in the norepinephrine transporter, rs2242446 and rs5569, and the retardation symptoms of major depressive disorder using quantitative trait testing with the UNPHASED program, rs5569 was associated with depressed mood, and the GG genotype may be a risk factor for this; rs2242446 was associated with work and interest, and the TT genotype may be a risk factor for loss of interest. Our findings suggest that rs2242446 and rs5569 in the norepinephrine transporter gene are associated with the retardation symptoms of depression in the Hart Chinese population.
文摘According to the published gene sequence of the major surface antigen (P30) of Toxoplasma gondii, a pair of primers were designed and synthesized. Using polymerase chain reaction (PCR), the coding sequences of P30 gene were amplified from a Chinese strain of T. gondii, The amplified gene fragment and plasmid pB220 were digested with EcoRI and BamHI and then ligated. The inserted gene fragment was sequenced by the chain termination method, the reading reveals that nucleotide sequence determined was the same as the P30 sequecne of RH strain pubilished by Burg (1988), except that one base was changed. The recombinant plasmid containing P30 gene was transformed to E. coli DH5α.After temperature inducing culture, the total cellular proteins were analysed by SDS-PAGE and Western blot. The results show that the p30 gene cloned into the plasmid could express in E. coli, and the expression product had immunogenicity.
基金Supported by National High Technology Research and Development Program of China(863 Program)(2011AA10A106)Yunnan Provincial Fund for Applied Basic Researches(2010CC001)Key New Product Development Plan of Yunnan Province(2012BB015)~~
文摘A DH population derived from C49S-87/01Y1-1069 was used to study the inheritance of wheat haploid embryo production frequency（EPF） in wheat × maize cross with the mixed major gene and polygene inheritance model of quantitative traits. The results showed that the EPF of wheat × maize cross was controlled by two dominant epistatic genes and polygene with gene effects of 1.95 for the first major gene, 6.69 for the second one and 2.80 for the polygene. The inheritability of major genes was as high as 72.09%, suggesting that the differences in EPF among wheat materials were mainly influenced by genotype. However, non-genetic factors were still important, especially for wheat materials with low EPF.
