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Genome-wide association and linkage mapping strategies reveal genetic loci and candidate genes of phosphorus utilization in soybean 被引量:1
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作者 ZHANG Hua WU Hai-yan +7 位作者 TIAN Rui KONG You-bin CHU Jia-hao XING Xin-zhu DU Hui JIN Yuan LI Xi-huan ZHANG Cai-ying 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2022年第9期2521-2537,共17页
Insufficient available phosphorus in soil has become an important limiting factor for the improvement of yield and quality in soybean. The mining of QTLs and candidate genes controlling soybean phosphorus utilization ... Insufficient available phosphorus in soil has become an important limiting factor for the improvement of yield and quality in soybean. The mining of QTLs and candidate genes controlling soybean phosphorus utilization related traits is a necessary strategy to solve this problem. In this study, 11 phosphorus utilization related traits of a natural population of 281 typical soybean germplasms and a recombinant inbred line(RIL) population of 270 lines were evaluated under different phosphorus conditions at two critical stages: the four-leaf stage as the seedling critical stage was designated as the Tstage, and the six-leaf stage as the flowering critical stage was designated as the Tstage. In total, 200 single nucleotide polymorphism(SNP) loci associated with phosphorus utilization related traits were identified in the natural population, including 91 detected at the Tstage, and 109 detected at the Tstage. Among these SNP loci, one SNP cluster(s715611375, ss715611377, ss715611379 and ss715611380) on Gm12 was shown to be significantly associated with plant height under the low phosphorus condition at the Tstage, and the elite haplotype showed significantly greater plant height than the others. Meanwhile, one pleiotropic SNP cluster(ss715606501, ss715606506 and ss715606543) on Gm10 was found to be significantly associated with the ratio of root/shoot, root and total dry weights under the low phosphorus condition at the Tstage, and the elite haplotype also presented significantly higher values for related characteristics under the phosphorus starvation condition. Furthermore, four co-associated SNP loci(ss715597964, ss715607012, ss715622173 and ss715602331) were identified under the low phosphorus condition at both the Tand Tstages, and 12 QTLs were found to be consistent with these genetic loci in the RIL population. More importantly, 14 candidate genes, including MYB transcription factor, purple acid phosphatase, sugar transporter and HSP20-like chaperones superfamily genes, etc., showed differential expression levels after low phosphorus treatment, and three of them were further verified by q RT-PCR. Thus, these genetic loci and candidate genes could be applied in markerassisted selection or map-based gene cloning for the genetic improvement of soybean phosphorus utilization. 展开更多
关键词 SOYBEAN phosphorus utilization GWAS linkage mapping genetic loci candidate genes
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Identification of QTLs and candidate genes for physiological traits associated with drought tolerance in cotton 被引量:5
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作者 MAGWANGA Richard Odongo LU Pu +5 位作者 KIRUNGU Joy Nyangasi CAI Xiaoyan ZHOU Zhongli AGONG Stephen Gaya WANG Kunbo LIU Fang 《Journal of Cotton Research》 2020年第1期13-45,共33页
Background:Cotton is mainly grown for its natural fiber and edible oil.The fiber obtained from cotton is the indispensable raw material for the textile industries.The ever changing climatic condition,threatens cotton ... Background:Cotton is mainly grown for its natural fiber and edible oil.The fiber obtained from cotton is the indispensable raw material for the textile industries.The ever changing climatic condition,threatens cotton production due to a lack of sufficient water for its cultivation.Effects of drought stress are estimated to affect more than 50%of the cotton growing regions.To elucidate the drought tolerance phenomenon in cotton,a backcross population was developed from G.tomentosum,a drought tolerant donor parent and G.hirsutum which is highly susceptible to drought stress.Results:A genetic map of 10888 SNP markers was developed from 200 BC_2F_2 populations.The map spanned 4191.3 centi-Morgan(c M),with an average distance of 0.1047 c M,covering 51%and 49%of At and Dt sub genomes,respectively.Thirty stable Quantitative trait loci(QTLs)were detected,in which more than a half were detected in the At subgenome.Eighty-nine candidate genes were mined within the QTL regions for three traits:cell membrane stability(CMS),saturated leaf weight(SLW)and chlorophyll content.The genes had varied physiochemical properties.A majority of the genes were interrupted by introns,and only 15 genes were intronless,accounting for 17%of the mined genes.The genes were found to be involved molecular function(MF),cellular component(CC)and biological process(BP),which are the main gene ontological(GO)functions.A number of mi RNAs were detected,such as mi R164,which is associated with NAC and MYB genes,with a profound role in enhancing drought tolerance in plants.Through RT-q PCR analysis,5 genes were found to be the key genes involved in enhancing drought tolerance in cotton.Wild cotton harbors a number of favorable alleles,which can be exploited to aid in improving the narrow genetic base of the elite cotton cultivars.The detection of 30 stable QTLs and 89 candidate genes found to be contributed by the donor parent,G.tomentosum,showed the significant genes harbored by the wild progenitors which can be exploited in developing more robust cotton genotypes with diverse tolerance levels to various environmental stresses.Conclusion:This was the first study involving genome wide association mapping for drought tolerance traits in semi wild cotton genotypes.It offers an opportunity for future exploration of these genes in developing highly tolerant cotton cultivars to boost cotton production. 展开更多
关键词 COTTON spp. Quantitative TRAIT loci genetic map Drought tolerance mi RNAS Gene ontology
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果树分子遗传图谱研究进展 被引量:4
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作者 高佳 汤浩茹 +1 位作者 董晓莉 罗娅 《北方园艺》 CAS 北大核心 2007年第10期44-50,共7页
概述了果树分子遗传图谱构建的理论基础、一般程序和方法,针对作图群体类型、群体大小的确定、分子标记技术的选择和偏分离标记位点的处理等问题进行了讨论。介绍了质量和数量性状基因位点定位、基因组比较作图、标记辅助选择育种和基... 概述了果树分子遗传图谱构建的理论基础、一般程序和方法,针对作图群体类型、群体大小的确定、分子标记技术的选择和偏分离标记位点的处理等问题进行了讨论。介绍了质量和数量性状基因位点定位、基因组比较作图、标记辅助选择育种和基因定位克隆技术在果树分子遗传图谱上的应用及其所构建的分子遗传图谱,指出了今后果树分子遗传图谱研究的重点。 展开更多
关键词 分子遗传图谱 基因定位 比较作图 MAS 基因克隆
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cDNA-AFLP分子标记技术及其在茶树育种上的应用前景 被引量:4
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作者 王会 梁月荣 +2 位作者 陆建良 张广辉 孙庆磊 《茶叶》 2006年第2期75-78,共4页
cDNA-AFLP技术是一种新的分子标记技术,该技术结合了AFLP和mRNA差异显示技术,具有可靠性和高效性且不需要预先知道序列信息,广泛应用于植物生长发育过程基因分离及表达特性的研究。本文阐述了分子标记技术cDNA-AFLP的原理及程序并介绍... cDNA-AFLP技术是一种新的分子标记技术,该技术结合了AFLP和mRNA差异显示技术,具有可靠性和高效性且不需要预先知道序列信息,广泛应用于植物生长发育过程基因分离及表达特性的研究。本文阐述了分子标记技术cDNA-AFLP的原理及程序并介绍了它的应用情况。目前cDNA-AFLP技术已经在茶树基因表达特性研究上作了一些尝试,随着该技术的不断发展完善在茶树育种上有广阔的应用前景。 展开更多
关键词 茶树育种 CDNA-AFLP 分子标记 基因表达 基因克隆
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高粱粒重遗传研究进展 被引量:4
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作者 韩立杰 才宏伟 《生物技术通报》 CAS CSCD 北大核心 2019年第5期15-27,共13页
高粱是世界第五大粮食作物,种植并培育高产的高粱品种对缓解世界粮食安全问题具有重要意义。粒重是构成产量的一个重要因素,增加粒重是提高高粱产量的重要途径。粒重是由数量性状基因控制的复杂性状,目前已有部分控制高粱粒重的QTLs(Qua... 高粱是世界第五大粮食作物,种植并培育高产的高粱品种对缓解世界粮食安全问题具有重要意义。粒重是构成产量的一个重要因素,增加粒重是提高高粱产量的重要途径。粒重是由数量性状基因控制的复杂性状,目前已有部分控制高粱粒重的QTLs(Quantitative trait loci,QTL)被定位,这些QTLs在高粱10条染色体上均有分布。对高粱粒重的遗传特点,粒重的影响因素及粒重QTL定位的研究进展进行了总结和概述,对已定位的高粱粒重QTLs进行了比较分析,查找了水稻和玉米中已克隆的粒重相关基因在高粱中的同源基因,并与高粱粒重QTLs定位区间进行了比较,以期为高粱粒重的分子标记辅助育种及高粱粒重主效QTLs的精细定位及克隆提供依据。 展开更多
关键词 高粱 粒重 遗传分析 基因定位 数量性状基因座
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玉米DNA限制性片段长度多态(RFLPs)的研究 被引量:2
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作者 季良越 罗福和 《河南农业大学学报》 CAS CSCD 1993年第2期120-126,共7页
文章简要介绍了10余年来有关玉米DNA限制性片段长度多态(RFLPs)的研究,说明RFLP的分析原理和特点,构建玉米RFLP连锁图的方法,以及玉米RFLPs在遗传育种中的主要应用。
关键词 杂交 数量性状 遗传 玉米
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小麦数量性状基因定位及比较基因组研究进展 被引量:5
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作者 许自成 池振中 +1 位作者 贾志强 许时伦 《河南农业大学学报》 CAS CSCD 1997年第4期327-333,共7页
综述了小麦数量性状基因定位的研究概况;分析了提高小麦QTL检测效率的途径,概述了小麦与其它禾本科作物之间关于比较基因组方面的研究进展。此外,还就分子标记等有关问题进行了讨论。
关键词 小麦 基因定位 基因组 遗传标记
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Progress in the Study of Molecular Genetic Improvements of Poplar in China 被引量:4
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作者 Shan-Zhi Lin Zhi-Yi Zhang Qian Zhang Yuan-Zhen Lin 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2006年第9期1001-1007,共7页
The poplar is one of the most economically important and intensively studied tree species owing to its wide application in the timber industry and as a model material for the study of woody plants. The natural resourc... The poplar is one of the most economically important and intensively studied tree species owing to its wide application in the timber industry and as a model material for the study of woody plants. The natural resource of poplars in China is replete. Over the past 10 years, the application of molecular biological techniques to genetic improvements in poplar species has been widely studied in China. Recent advances in molecular genetic improvements of poplar, including cDNA library construction, gene cloning and identification, genetic engineering, gene expression, genetic linkage map construction, mapping of quantitative trait loci (QTL) and molecular-assisted selection, are reviewed in the present paper. In addition, the application of modern biotechnology to molecular improvements in the genetic traits of the poplar and some unsolved problems are discussed. 展开更多
关键词 POPLAR gene engineering genetic improvement genetic linkage maps molecular marker quantitative trait loci (QTL) mapping
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