[Objectives]The paper was to determine the extraction and refining process of polysaccharide from Tricholoma matsutake,and to verify its whitening effect.[Methods]With T.matsutake as the research object,the effects of...[Objectives]The paper was to determine the extraction and refining process of polysaccharide from Tricholoma matsutake,and to verify its whitening effect.[Methods]With T.matsutake as the research object,the effects of eluent concentration,loading amount and diameter-to-height ratio on the refining process of T.matsutake polysaccharide were explored by orthogonal test,to optimize the dynamic elution conditions of T.matsutake polysaccharide.The survival rate of mouse melanoma cells(B16),inhibition of melanin synthesis and inhibition of tyrosinase activity of crude extract A1 and refined extracts A2-A4 were tested to verify the whitening effect of T.matsutake.[Results]The best extraction and refining process for T.matsutake polysaccharide was:eluent 40%ethanol(volume fraction),loading amount 30 mg/g(polysaccharide-resin),diameter-to-height ratio 1∶8.Under this condition,the extraction rate of T.matsutake can reach up to 33%(A3).The whitening efficacy test showed that crude extract A1 and refined extracts A2-A4 had good whitening effects,and the whitening effects were A3≥A4>A1>A2.[Conclusions]The study provides a reference for the application of T.matsutake polysaccharide in cosmetics.展开更多
Taking refined flour,matsutake powder,and highland barley powder as main raw materials,this experiment studied the optimal formula of matsutake highland barley biscuit. Besides,single factor experiment was carried out...Taking refined flour,matsutake powder,and highland barley powder as main raw materials,this experiment studied the optimal formula of matsutake highland barley biscuit. Besides,single factor experiment was carried out for the amount of highland barley powder,white granulated sugar,and shortening. Then,the response surface optimization analysis was made on crispness and sensory score of the biscuit. The experiment indicates that taking the refined flour as the base 100 g( 100%),the formula of 20% highland barley powder,25% white granulated sugar,and 26% shortening can bake crisp biscuit with complete shape,pure flavor and high quality.展开更多
An ultrasonic-assisted technique was employed to extract crude polysaccharide from Tricholomamatsutake fruiting bodies. Single-factor tests and orthogonal experimental design （L9（33）） were used to obtain theoptima...An ultrasonic-assisted technique was employed to extract crude polysaccharide from Tricholomamatsutake fruiting bodies. Single-factor tests and orthogonal experimental design （L9（33）） were used to obtain theoptimal extraction conditions. Results showed that the optimal parameters were as follows： ultrasonic temperature,40℃; ultrasonic time, 50 min; water to raw material ratio, 25 ml/g; ultrasonic frequency, 45 kHz; and ultrasonic power,100 W. Three novel T. matsutake polysacchadde （TMP） fractions （TMP30, TMP60, and TMP80） were isolated andpurified from TMP by stepwise alcohol precipitation. Their preliminary structural features were determined by high-performance anion-exchange chromatography with pulsed-amperometric detection （HPAEC-PAD） and Fouriertransform infrared spectrophotometer （FT-IR） analyses. Furthermore, their in vitro antioxidant activity was investigatedin terms of a reducing power assay and the scavenging rates of 2,2-diphenyl-l-picrylhydrazyl （DPPH） and hydroxylradicals. The order of the various fractions based on their antioxidant activity was TMP80〉TMP〉TMP60〉TMP30.These findings suggested that novel polysaccharide fractions from T. matsutake, especially TMP80, could be prom-ising active macromolecules for biomedical use.展开更多
Climate change affects various facets of life but there is little data on its effects on wild mushroom fruiting.Yunnan Province in China is a rich source of wild mushrooms and has experienced a temperature rise over r...Climate change affects various facets of life but there is little data on its effects on wild mushroom fruiting.Yunnan Province in China is a rich source of wild mushrooms and has experienced a temperature rise over recent decades.This has resulted in warmer temperatures but the impacts of these changes on mushroom production lack documentation.We collected data on the fruiting of the highly prized matsutake mushroom(Tricholoma matsutake)in West Yunnan,China over an 11 year period from 2000 to 2010.Fruiting phenology and productivity were compared against the driving meteorological variables using Projection to Latent Structure regression.The mushrooms appeared later in the season during the observation period,which is most likely explained by rising temperatures and reduced rain during May and June.High temperature and abundant rain in August resulted in good productivity.The climate response of matsutake production results from a sequence of processes that are possibly linked with regulatory signals and resource availability.To advance the knowledge of this complex system,a holistic research approach integrating biology,ecology,genetics,physiology,and phytochemistry is needed.Our results contribute to a general model of fungal ecology,which can be used to predict the responses of fungi to global climate change.展开更多
Song Rong (i.e. Matsutake, Tricholoma matsutake) is the most favorite edible mushroom in Japan and restrictedly distributes in forests in East Asia. The yield of Song Rong, however, has been extremely dropping since 1...Song Rong (i.e. Matsutake, Tricholoma matsutake) is the most favorite edible mushroom in Japan and restrictedly distributes in forests in East Asia. The yield of Song Rong, however, has been extremely dropping since 1940 's in the region due to changes of environmental ecology of nature forests. As a compensation for consumption, Japan imported products of T. matsutake from Korea and China, and T. magnivelare from North America since mid-1970s, but T. matsutake stili accounts for a large portion of the ...展开更多
文摘[Objectives]The paper was to determine the extraction and refining process of polysaccharide from Tricholoma matsutake,and to verify its whitening effect.[Methods]With T.matsutake as the research object,the effects of eluent concentration,loading amount and diameter-to-height ratio on the refining process of T.matsutake polysaccharide were explored by orthogonal test,to optimize the dynamic elution conditions of T.matsutake polysaccharide.The survival rate of mouse melanoma cells(B16),inhibition of melanin synthesis and inhibition of tyrosinase activity of crude extract A1 and refined extracts A2-A4 were tested to verify the whitening effect of T.matsutake.[Results]The best extraction and refining process for T.matsutake polysaccharide was:eluent 40%ethanol(volume fraction),loading amount 30 mg/g(polysaccharide-resin),diameter-to-height ratio 1∶8.Under this condition,the extraction rate of T.matsutake can reach up to 33%(A3).The whitening efficacy test showed that crude extract A1 and refined extracts A2-A4 had good whitening effects,and the whitening effects were A3≥A4>A1>A2.[Conclusions]The study provides a reference for the application of T.matsutake polysaccharide in cosmetics.
基金Supported by Agricultural Product Processing Technology and Product Development Project of the 13th Five-Year Plan of Tibet
文摘Taking refined flour,matsutake powder,and highland barley powder as main raw materials,this experiment studied the optimal formula of matsutake highland barley biscuit. Besides,single factor experiment was carried out for the amount of highland barley powder,white granulated sugar,and shortening. Then,the response surface optimization analysis was made on crispness and sensory score of the biscuit. The experiment indicates that taking the refined flour as the base 100 g( 100%),the formula of 20% highland barley powder,25% white granulated sugar,and 26% shortening can bake crisp biscuit with complete shape,pure flavor and high quality.
基金supported by the Natural Science Foundation of Shandong Province of China(No.ZR2015CL002)the Doctoral Research Startup Foundation of Liaocheng University(No.31805),China
文摘An ultrasonic-assisted technique was employed to extract crude polysaccharide from Tricholomamatsutake fruiting bodies. Single-factor tests and orthogonal experimental design （L9（33）） were used to obtain theoptimal extraction conditions. Results showed that the optimal parameters were as follows： ultrasonic temperature,40℃; ultrasonic time, 50 min; water to raw material ratio, 25 ml/g; ultrasonic frequency, 45 kHz; and ultrasonic power,100 W. Three novel T. matsutake polysacchadde （TMP） fractions （TMP30, TMP60, and TMP80） were isolated andpurified from TMP by stepwise alcohol precipitation. Their preliminary structural features were determined by high-performance anion-exchange chromatography with pulsed-amperometric detection （HPAEC-PAD） and Fouriertransform infrared spectrophotometer （FT-IR） analyses. Furthermore, their in vitro antioxidant activity was investigatedin terms of a reducing power assay and the scavenging rates of 2,2-diphenyl-l-picrylhydrazyl （DPPH） and hydroxylradicals. The order of the various fractions based on their antioxidant activity was TMP80〉TMP〉TMP60〉TMP30.These findings suggested that novel polysaccharide fractions from T. matsutake, especially TMP80, could be prom-ising active macromolecules for biomedical use.
基金sponsored jointly by the National Natural Science Foundation of China(Grant No.30800158)the 11th Five-Year China Key Science&Technology Project on Silviculture for Carbon Sequestration in Subtropics(Grant No:2008BAD95B09)+3 种基金the Ford Foundation(Grant No.10850639)the National Research Council of Thailand(grant NRCT/55201020007)Mae Fah Luang University(grant MFU/54101020048)King Saud University for support.
文摘Climate change affects various facets of life but there is little data on its effects on wild mushroom fruiting.Yunnan Province in China is a rich source of wild mushrooms and has experienced a temperature rise over recent decades.This has resulted in warmer temperatures but the impacts of these changes on mushroom production lack documentation.We collected data on the fruiting of the highly prized matsutake mushroom(Tricholoma matsutake)in West Yunnan,China over an 11 year period from 2000 to 2010.Fruiting phenology and productivity were compared against the driving meteorological variables using Projection to Latent Structure regression.The mushrooms appeared later in the season during the observation period,which is most likely explained by rising temperatures and reduced rain during May and June.High temperature and abundant rain in August resulted in good productivity.The climate response of matsutake production results from a sequence of processes that are possibly linked with regulatory signals and resource availability.To advance the knowledge of this complex system,a holistic research approach integrating biology,ecology,genetics,physiology,and phytochemistry is needed.Our results contribute to a general model of fungal ecology,which can be used to predict the responses of fungi to global climate change.
文摘Song Rong (i.e. Matsutake, Tricholoma matsutake) is the most favorite edible mushroom in Japan and restrictedly distributes in forests in East Asia. The yield of Song Rong, however, has been extremely dropping since 1940 's in the region due to changes of environmental ecology of nature forests. As a compensation for consumption, Japan imported products of T. matsutake from Korea and China, and T. magnivelare from North America since mid-1970s, but T. matsutake stili accounts for a large portion of the ...
