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Conditioned medium from human dental pulp stem cells treats spinal cord injury by inhibiting microglial pyroptosis
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作者 Tao Liu Ziqian Ma +8 位作者 Liang Liu Yilun Pei Qichao Wu Songjie Xu Yadong Liu Nan Ding Yun Guan Yan Zhang Xueming Chen 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第5期1105-1111,共7页
Human dental pulp stem cell transplantation has been shown to be an effective therapeutic strategy for spinal cord injury.However,whether the human dental pulp stem cell secretome can contribute to functional recovery... Human dental pulp stem cell transplantation has been shown to be an effective therapeutic strategy for spinal cord injury.However,whether the human dental pulp stem cell secretome can contribute to functional recovery after spinal cord injury remains unclear.In the present study,we established a rat model of spinal cord injury based on impact injury from a dropped weight and then intraperitoneally injected the rats with conditioned medium from human dental pulp stem cells.We found that the conditioned medium effectively promoted the recovery of sensory and motor functions in rats with spinal cord injury,decreased expression of the microglial pyroptosis markers NLRP3,GSDMD,caspase-1,and interleukin-1β,promoted axonal and myelin regeneration,and inhibited the formation of glial scars.In addition,in a lipopolysaccharide-induced BV2 microglia model,conditioned medium from human dental pulp stem cells protected cells from pyroptosis by inhibiting the NLRP3/caspase-1/interleukin-1βpathway.These results indicate that conditioned medium from human dental pulp stem cells can reduce microglial pyroptosis by inhibiting the NLRP3/caspase-1/interleukin-1βpathway,thereby promoting the recovery of neurological function after spinal cord injury.Therefore,conditioned medium from human dental pulp stem cells may become an alternative therapy for spinal cord injury. 展开更多
关键词 BV2 conditioned medium dental pulp stem cells GSDMD MICROGLIA NEUROINFLAMMATION NLRP3 PYROPTOSIS spinal cord injury
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THE INTERIOR TRANSMISSION EIGENVALUE PROBLEM FOR AN ANISOTROPIC MEDIUM BY A PARTIALLY COATED BOUNDARY
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作者 向建立 严国政 《Acta Mathematica Scientia》 SCIE CSCD 2024年第1期339-354,共16页
We consider the interior transmission eigenvalue problem corresponding to the scattering for an anisotropic medium of the scalar Helmholtz equation in the case where the boundary?Ωis split into two disjoint parts and... We consider the interior transmission eigenvalue problem corresponding to the scattering for an anisotropic medium of the scalar Helmholtz equation in the case where the boundary?Ωis split into two disjoint parts and possesses different transmission conditions.Using the variational method,we obtain the well posedness of the interior transmission problem,which plays an important role in the proof of the discreteness of eigenvalues.Then we achieve the existence of an infinite discrete set of transmission eigenvalues provided that n≡1,where a fourth order differential operator is applied.In the case of n■1,we show the discreteness of the transmission eigenvalues under restrictive assumptions by the analytic Fredholm theory and the T-coercive method. 展开更多
关键词 interior transmission eigenvalue anisotropic medium partially coated boundary the analytic Fredholm theory T-coercive method
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Adenosine A_(2A)receptor blockade attenuates excitotoxicity in rat striatal medium spiny neurons during an ischemic-like insult
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作者 Elisabetta Coppi Federica Cherchi Alasdair J.Gibb 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第2期255-257,共3页
During brain ischemia,excitotoxicity and peri-infarct depolarization injuries occur and cause cerebral tissue damage.Indeed,anoxic depolarization,consisting of massive neuronal depolarization due to the loss of membra... During brain ischemia,excitotoxicity and peri-infarct depolarization injuries occur and cause cerebral tissue damage.Indeed,anoxic depolarization,consisting of massive neuronal depolarization due to the loss of membrane ion gradients,occurs in vivo or in vitro during an energy failure.The neuromodulator adenosine is released in huge amounts during cerebral ischemia and exerts its effects by activating specific metabotropic receptors,namely:A_(1),A_(2A),A_(2B),and A_(3).The A_(2A)receptor subtype is highly expressed in striatal medium spiny neurons,which are particularly susceptible to ischemic damage.Evidence indicates that the A2Areceptors are upregulated in the rat striatum after stroke and the selective antagonist SCH58261 protects from exaggerated glutamate release within the first 4 hours from the insult and alleviates neurological impairment and histological injury in the following 24 hours.We recently added new knowledge to the mechanisms by which the adenosine A2Areceptor subtype participates in ischemia-induced neuronal death by performing patch-clamp recordings from medium spiny neurons in rat striatal brain slices exposed to oxygen and glucose deprivation.We demonstrated that the selective block of A2Areceptors by SCH58261 significantly reduced ionic imbalance and delayed the anoxic depolarization in medium spiny neurons during oxygen and glucose deprivation and that the mechanism involves voltage-gated K+channel modulation and a presynaptic inhibition of glutamate release by the A2Areceptor antagonist.The present review summarizes the latest findings in the literature about the possibility of developing selective ligands of A2Areceptors as advantageous therapeutic tools that may contribute to counteracting neurodegeneration after brain ischemia. 展开更多
关键词 adenosine A_(2A)receptors anoxic depolarization brain ischemia glutamate excitotoxicity medium spiny neurons oxygen and glucose deprivation
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A Comparison Study on the δD Value of Polycyclic Aromatic Hydrocarbons in Hydrous Pyrolysate of Herbaceous Peat with Different Water Medium
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作者 DUAN Yi WU Yingzhong +5 位作者 XING Lantian WU Yingqin ZHAO Yang LI Zhongping MA Lanhua DUAN Mingchen 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2023年第6期1734-1743,共10页
To investigate the influence of diagenetic water media on the hydrogen isotopes of individual sedimentary aromatic compounds,a series of hydrous pyrolyses were conducted on herbaceous peat.Polycyclic aromatic hydrocar... To investigate the influence of diagenetic water media on the hydrogen isotopes of individual sedimentary aromatic compounds,a series of hydrous pyrolyses were conducted on herbaceous peat.Polycyclic aromatic hydrocarbons(PAHs)in hydrous pyrolysed samples and their hydrogen isotopic composition characteristics were studied.The aqueous medium demonstrated a significant influence on the hydrogen isotopic composition of the individual PAHs generated during pyrolysis.The results showed that the PAHs formed after pyrolysis in the presence of a saltwater medium with high δD value from a salt lake had a heavy hydrogen isotopic composition.The PAHs formed after pyrolysis in the presence of a fresh water medium with low δD value from a swamp had a light hydrogen isotopic composition.The difference in the average PAHδD value between the two hydrous experiments varied from -174‰ to -109‰,suggesting that the hydrogen isotopic composition of individual sedimentary PAHs can reflect the source of the diagenetic water medium.In addition,a comparative study found that the hydrogen isotopes of PAHs were superior to those of n-alkanes in the same sample for diagenetic water indications.The results indicated that the exchange of water-derived inorganic hydrogen and organic hydrogen was more intensive in freshwater experiments than in saltwater experiments.With an increase in the simulation temperature,the average δD value of PAHs generated in the hydrous simulation experiments showed an increasing trend,reflecting that the δD value of sedimentary PAHs formed with the participation of diagenetic water media was still closely related to the thermal maturity of organic matter.Comparative studies showed that theδD values of different types of organic compounds produced by hydrous pyrolysis of peat were in the order,PAHs>n-alkanes>methane. 展开更多
关键词 hydrogen isotope PAHS hydrous pyrolysis herbaceous peat water medium
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Flow and heat transfer of a nanofluid through a porous medium due to stretching/shrinking sheet with suction,magnetic field and thermal radiation
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作者 Ubaidullah Yashkun Khairy Zaimi +2 位作者 Suliadi Firdaus Sufahani Mohamed REid Mohammad Ferdows 《Applied Mathematics(A Journal of Chinese Universities)》 SCIE CSCD 2023年第3期373-391,共19页
This study investigates the suction and magnetic field effects on the two-dimensional nanofluid flow through a stretching/shrinking sheet at the stagnation point in the porous medium with thermal radiation.The governi... This study investigates the suction and magnetic field effects on the two-dimensional nanofluid flow through a stretching/shrinking sheet at the stagnation point in the porous medium with thermal radiation.The governing partial differential equations(PDEs)are converted into ordinary differential equations(ODEs)using the similarity transformation.The resulting ODEs are then solved numerically by using the bvp4c solver in MATLAB software.It was found that dual solutions exist for the shrinking parameter values up to a certain range.The numerical results obtained are compared,and the comparison showed a good agreement with the existing results in the literature.The governing parameters’effect on the velocity,temperature and nanoparticle fraction fields as well as the skin friction coefficient,the local Nusselt number and the Sherwood number are represented graphically and analyzed.The variation of the velocity,temperature and concentration increase with the increase in the suction and magnetic field parameters.It seems that the thermal radiation effect has increased the local Sherwood number while the local Nusselt number is reduced with it. 展开更多
关键词 stagnation point flow NANOFLUID porous medium SUCTION MAGNETOHYDRODYNAMIC thermal radiation
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2.5-Dimensional modeling of EM logging-while-drilling tool in anisotropic medium on a Lebedev grid
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作者 Zhen-Guan Wu Hu Li Xi-Zhou Yue 《Petroleum Science》 SCIE EI CAS CSCD 2023年第1期249-260,共12页
A 2.5D finite-difference(FD)algorithm for the modeling of the electromagnetic(EM)logging-whiledrilling(LWD)tool in anisotropic media is presented.The FD algorithm is based on the Lebedev grid,which allows for the disc... A 2.5D finite-difference(FD)algorithm for the modeling of the electromagnetic(EM)logging-whiledrilling(LWD)tool in anisotropic media is presented.The FD algorithm is based on the Lebedev grid,which allows for the discretization of the frequency-domain Maxwell's equations in the anisotropic media in 2.5D scenarios without interpolation.This leads to a system of linear equations that is solved using the multifrontal direct solver which enables the simulation of multi-sources at nearly the cost of simulating a single source for each frequency.In addition,near-optimal quadrature derived from an optimized integration path in the complex plane is employed to implement the fast inverse Fourier Transform(IFT).The algorithm is then validated by both analytic and 3D solutions.Numerical results show that two Lebedev subgrid sets are sufficient for TI medium,which is common in geosteering environments.The number of quadrature points is greatly reduced by using the near-optimal quadrature method. 展开更多
关键词 2.5D modeling Electromagnetic logging-while-drilling Anisotropic medium Lebedev grid
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INVERSE CONDUCTIVE MEDIUM SCATTERING WITH UNKNOWN BURIED OBJECTS
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作者 曲风龙 贾瑞雪 崔艳丽 《Acta Mathematica Scientia》 SCIE CSCD 2023年第5期2005-2025,共21页
This paper is concerned with inverse acoustic scattering in an inhomogeneous medium with a conductive boundary condition and the unknown buried impenetrable objects inside.Using a variational approach,we establish the... This paper is concerned with inverse acoustic scattering in an inhomogeneous medium with a conductive boundary condition and the unknown buried impenetrable objects inside.Using a variational approach,we establish the well-posedness of the direct problem.For the inverse problem,we shall numerically reconstruct the inhomogeneous medium from the far-field data for different kinds of cases.For the case when a Dirichlet boundary condition is imposed on the buried object,the classical factorization method proposed in[1]is justified as valid for reconstructing the inhomogeneous medium from the far-field data.For the case when a Neumann boundary condition is imposed on the buried object,the classical factorization method of[1]cannot be applied directly,since the middle operator of the factorization of the far-field operator is only compact.In this case,we develop a modified factorization method to locate the inhomogeneous medium with a conductive boundary condition and the unknown buried objects.Some numerical experiments are provided to demonstrate the practicability of the inversion algorithms developed. 展开更多
关键词 inverse acoustic scattering factorization method numerical reconstruction inhomogeneous medium
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Aerodynamic shape and drag scaling law of a flexible fibre in a flowing medium
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作者 Bo-Hua Sun Xiao-Lin Guo 《Theoretical & Applied Mechanics Letters》 CAS CSCD 2023年第3期159-163,共5页
The study of a flexible body immersed in a flowing medium is one of the best way to find its aerodynamic shape.This Letter revisited the problem that was first studied by Alben et al.(Nature 420,479–481,2002).To dete... The study of a flexible body immersed in a flowing medium is one of the best way to find its aerodynamic shape.This Letter revisited the problem that was first studied by Alben et al.(Nature 420,479–481,2002).To determine the aerodynamic shape of the fibre,a simpler approach is proposed.A universal drag scaling law is obtained and the universality of the Alben-Shelley-Zhang scaling law is confirmed by using dimensional analysis.A complete Maple code is provided for finding aerodynamic shape of the fibre in the flowing medium. 展开更多
关键词 Flexible fibre Flow medium Aerodynamic shape Drag Scaling law
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A novel binary effective medium model to describe the prepeak stressstrain relationship of combined bodies of rock-like material and rock
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作者 Tienan Wang Yue Zhai +2 位作者 Huan Gao Yubai Li Ruifeng Zhao 《International Journal of Mining Science and Technology》 SCIE EI CAS CSCD 2023年第5期601-616,共16页
Combined bodies of rock-like material and rock are widely encountered in geotechnical engineering,such as tunnels and mines.The existing theoretical models describing the stress-strain relationship of a combined body ... Combined bodies of rock-like material and rock are widely encountered in geotechnical engineering,such as tunnels and mines.The existing theoretical models describing the stress-strain relationship of a combined body lack a binary feature.Based on effective medium theory,this paper presents the governing equation of the“elastic modulus”for combined and single bodies under triaxial compressive tests.A binary effective medium model is then established.Based on the compressive experiment of concretegranite combined bodies,the feasibility of determining the stress threshold based on crack axial strain is discussed,and the model is verified.The model is further extended to coal-rock combined bodies of more diverse types,and the variation laws of the compressive mechanical parameters are then discussed.The results show that the fitting accuracy of the model with the experimental curves of the concretegranite combined bodies and various types of coal-rock combined bodies are over 95%.The crack axial strain method can replace the crack volumetric strain method,which clarifies the physical meanings of the model parameters.The variation laws of matrix parameters and crack parameters are discussed in depth and are expected to be more widely used in geotechnical engineering. 展开更多
关键词 Combined body stress-strain relationship Hooke’s law Effective medium theory Stress threshold determination
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Principle of subtraction ghost imaging in scattering medium
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作者 付芹 白艳锋 +3 位作者 谭威 黄贤伟 南苏琴 傅喜泉 《Chinese Physics B》 SCIE EI CAS CSCD 2023年第6期250-254,共5页
Scattering medium in light path will cause distortion of the light field,resulting in poor signal-to-noise ratio(SNR)of ghost imaging.The disturbance is usually eliminated by the method of pre-compensation.We deduce t... Scattering medium in light path will cause distortion of the light field,resulting in poor signal-to-noise ratio(SNR)of ghost imaging.The disturbance is usually eliminated by the method of pre-compensation.We deduce the intensity fluctuation correlation function of the ghost imaging with the disturbance of the scattering medium,which proves that the ghost image consists of two correlated results:the image of scattering medium and the target object.The effect of the scattering medium can be eliminated by subtracting the correlated result between the light field after the scattering medium and the reference light from ghost image,which verifies the theoretical results.Our research may provide a new idea of ghost imaging in harsh environment. 展开更多
关键词 ghost imaging image reconstruction techniques scattering medium
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Analysis of a Stagnation Point Flow with Hybrid Nanoparticles over a Porous Medium
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作者 U.S.Mahabaleshwar T.Anusha M.Hatami 《Fluid Dynamics & Materials Processing》 EI 2023年第2期541-567,共27页
The unsteady stagnation-point flow of a hybrid nanofluid over a stretching/shrinking sheet embedded in a porous medium with mass transpiration and chemical reactions is considered.The momentum and mass transfer proble... The unsteady stagnation-point flow of a hybrid nanofluid over a stretching/shrinking sheet embedded in a porous medium with mass transpiration and chemical reactions is considered.The momentum and mass transfer problems are combined to form a system of partial differential equations,which is converted into a set of ordinary differential equations via similarity transformation.These ordinary differential equations are solved analytically to obtain the solution for velocity and concentration profiles in exponential and hypergeometric forms,respectively.The concentration profile is obtained for four different cases namely constant wall concentration,uniform mass flux,general power law wall con-centration and general power law mass flux.The effect of different physical parameters such as Darcy number Da^(1-1),mass transpiration parameter V_(C),stretching/shrinking parameter (d),chemical reaction parameter(β)and Schmidt number (Sc)on velocity and concentration profile is examined.Results show that,the axial velocity will decreases as the shrinking sheet parameter increases,regardless of whether the suction or injection case is examined.The concentration decreases with an increase in the shrinking sheet parameter and the chemical reaction rate parameter. 展开更多
关键词 Chemical reaction parameter mass transpiration hybrid nanofluid porous medium Schmidt number
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Influence of heat treatment processing on the microstructure and properties in medium manganese steel
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作者 ZHANG Yulong ZHANG Hanlong WANG Li 《Baosteel Technical Research》 CAS 2023年第1期17-21,共5页
For the purpose of developing a 1 500 MPa grade steel sheet with excellent strength and ductility, a 0.15C-10Mn-1.5Al steel was employed to study austenite stability and microstructural evolution based on a novel doub... For the purpose of developing a 1 500 MPa grade steel sheet with excellent strength and ductility, a 0.15C-10Mn-1.5Al steel was employed to study austenite stability and microstructural evolution based on a novel double annealing processing.After a conventional intercritical annealing process, the sample was heated again to a temperature higher than A_(c3) for a very short time to generate austenite grains with different manganese content;thus, the microstructure of martensite plus austenite can be obtained at room temperature.The experimental results show that with increasing annealing temperature, the tensile strength and yield strength increase.When the annealing temperature was higher than 820 ℃,the microstructure consisted of martensite plus austenite, and the tensile strength almost remained invariant with the annealing temperature.A tensile strength of 1 537 MPa and an elong-ation of 25.1% were achieved for the 820 ℃ condition.The volume fractions of austenite and martensite were identified by X-ray diffraction.It was found that with increasing annealing temperature, the volume fraction of the retained austenite decreased, and the ductility also had a gradual downward trend.The related austenite stability was discussed here as well. 展开更多
关键词 medium Mn steel cold-rolled AUSTENITE MARTENSITE
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Corrosion Mechanism of Alumina-magnesia Dry Materials for Smelting Manganese/chromium Steel in Coreless Medium Frequency Induction Furnaces
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作者 LIU Chenchen HUANG Ao +3 位作者 NIE Jianhua GU Huazhi QIN Chuanjiang Lidah Mpoli NACHILIMA 《China's Refractories》 CAS 2023年第4期8-15,共8页
Alumina-magnesia dry materials are widely used in induction furnace linings, but they show different kinds of damage when melting different kinds of alloy steel. In this paper, the chemical composition, phase composit... Alumina-magnesia dry materials are widely used in induction furnace linings, but they show different kinds of damage when melting different kinds of alloy steel. In this paper, the chemical composition, phase composition, and microstructure of the post-use dry materials for the working liners melting different kinds of steel were evaluated. Furthermore, the corrosion mechanism of the steel on the furnace lining materials was comprehensively analyzed. The findings reveal a significant ability of the Mn element in the molten steel to diffuse and penetrate into the refractories. Mn oxidizes to form MnO at the steel-refractory interface, and then forms a liquid phase with Al_(2)O_(3). The Cr element is dissolved into corundum and spinel of the refractories, resulting in lattice defects and structural damage of the materials. TiO2reacts with Al_(2)O_(3) to form Al_(2)TiO_(5), which plays a crucial role in preventing crack formation and propagation. Part of Ti4+dissolves into magnesia-alumina(MA), densifying the materials. TiO2also slows down the reaction between the Cr element and refractory components, further improving the corrosion resistance of the materials. 展开更多
关键词 coreless medium frequency induction furnace manganese/chromium steel alumina-magnesia dry materials sintering layer corrosion mechanism
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Slip Condition Effects on Unsteady MHD Fluid Flow with Radiative Heatflux over a Porous Medium
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作者 Abdullahi Ahmad Muhammad Nasir Sarki 《Advances in Pure Mathematics》 2023年第3期153-166,共14页
The objective of this paper is to study unsteady magneto hydrodynamic (MHD) free flow of viscoelastic fluid (Walter’s B) past an infinite vertical plate through porous medium. The temperature is assumed to be oscilla... The objective of this paper is to study unsteady magneto hydrodynamic (MHD) free flow of viscoelastic fluid (Walter’s B) past an infinite vertical plate through porous medium. The temperature is assumed to be oscillating with time. The solution obtained shows different profiles of effects of slip conditions on primary and secondary velocity. Also, the effects of various parameters on temperature, concentration, primary and secondary velocity profiles were presented graphically. The result indicated the secondary velocity is enhanced with increase in slip parameter. Primary velocity demonstrated opposite trend. 展开更多
关键词 Radiation Slip Parameter MHD Heat Flux and Porous medium
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Research on Well Testing Interpretation of Low Permeability Deformed Dual Medium Reservoir
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作者 Meinan Wang Yue Xie +2 位作者 Rui Zhang Guohao Zhang Jianguo Liu 《Open Journal of Applied Sciences》 2023年第11期2141-2148,共8页
Considering the influence of quadratic gradient term and medium deformation on the seepage equation, a well testing interpretation model for low permeability and deformation dual medium reservoirs was derived and esta... Considering the influence of quadratic gradient term and medium deformation on the seepage equation, a well testing interpretation model for low permeability and deformation dual medium reservoirs was derived and established. The difference method was used to solve the problem, and pressure and pressure derivative double logarithmic curves were drawn to analyze the seepage law. The research results indicate that the influence of starting pressure gradient and medium deformation on the pressure characteristic curve is mainly manifested in the middle and late stages. The larger the value, the more obvious the upward warping of the pressure and pressure derivative curve;the parameter characterizing the dual medium is the crossflow coefficient. The channeling coefficient determines the time and location of the appearance of the “concave”. The smaller the value, the later the appearance of the “concave”, and the more to the right of the “concave”. 展开更多
关键词 Low Permeability Oil Reservoirs Deformation medium Dual Media Cross Flow Coefficient Well Testing Interpretation Model
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The Reform Process of English Medium Instruction in Korean Higher Education
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作者 Ping Yu Na Yao Lili Cheng 《Journal of Contemporary Educational Research》 2023年第11期84-89,共6页
In the context of the internationalization of higher education,many non-English speaking countries,from Europe to Asia,are promoting English as a medium of instruction(EMI).The EMI in South Korea started earlier and d... In the context of the internationalization of higher education,many non-English speaking countries,from Europe to Asia,are promoting English as a medium of instruction(EMI).The EMI in South Korea started earlier and developed rapidly under the promotion.From the initial“Study Abroad in Korea Plan”to the World Class University(WCU)and Brain Korea 21st century(BK21)PLUS programs,with the support and guidance of the government,South Korea’s higher education has made significant progress,with the global top 100 universities in QS increasing from 1 to 6 in South Korea. 展开更多
关键词 English medium instruction EMI Internationalization of education Korean higher education
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Analysis of Forecast and Early Warning of Flood in Medium and Small Rivers
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作者 Yaxi Cai Xiaodong Yang Binhua Zhao 《Journal of Electronic Research and Application》 2023年第1期10-15,共6页
Flooding of small and medium rivers is caused by environmental factors like rainfall and soil loosening.With the development and application of technologies such as the Internet of Things and big data,the disaster sup... Flooding of small and medium rivers is caused by environmental factors like rainfall and soil loosening.With the development and application of technologies such as the Internet of Things and big data,the disaster supervision and management of large river basins in China has improved over the years.However,due to the frequent floods in small and medium-sized rivers in our country,the current prediction and early warning of small and medium-sized rivers is not accurate enough;it is difficult to realize real-time monitoring of small and medium-sized rivers,and it is also impossible to obtain corresponding data and information in time.Therefore,the construction and application of small and medium-sized river prediction and early warning systems should be further improved.This paper presents an analysis and discussion on flood forecasting and early warning systems for small and medium-sized rivers in detail,and corresponding strategies to improve the effect of forecasting and early warning systems are proposed. 展开更多
关键词 medium and small rivers Flood forecast and early warning Flood disaster
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烟碱降解细菌Ochrobactrum intermedium DN2生长培养基及培养条件的优化研究 被引量:9
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作者 袁勇军 黄丽金 陆兆新 《中国生物工程杂志》 CAS CSCD 北大核心 2005年第11期41-46,共6页
在烟碱生物降解的研究和实际应用中,需要在较短的时间内获得大量O.intermediumDN2菌体。为了提高菌株DN2的菌体浓度,缩短其培养时间,本研究采用PlackettBurman设计对影响O.intermediumDN2生长的内在和外在相关因素进行了评价。结果表明... 在烟碱生物降解的研究和实际应用中,需要在较短的时间内获得大量O.intermediumDN2菌体。为了提高菌株DN2的菌体浓度,缩短其培养时间,本研究采用PlackettBurman设计对影响O.intermediumDN2生长的内在和外在相关因素进行了评价。结果表明,影响菌株DN2生长的显著因素有胰蛋白胨、MgSO4·7H2O、初始pH值、温度、装液量和培养时间。在此基础上,采用响应曲面法分别对该菌生长培养基的组成和培养条件进行优化,得到最佳培养基组成为(g/L):胰蛋白胨11.34、牛肉膏3.00、NaCl5.00、MgSO4·7H2O3.71,pH值7.23;最佳培养条件为温度32℃、转速120r/min、装液量88ml/250ml三角瓶、培养时间34h。5L发酵罐验证实验表明,菌株DN2达到最大生长量的时间为36h,与预测值34h基本一致,比优化前缩短约12h;最大菌体浓度为6.49×109cfu/ml,与预测值6.73×109cfu/ml接近,比优化前高近一个数量级。 展开更多
关键词 O.intermedium DN2 培养基组成 培养条件 PLACKETT-BURMAN设计 响应曲面法
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IEEE 802.11e Medium Access Control层QoS机制的改进研究
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作者 周立衡 邱恭安 章国安 《南通大学学报(自然科学版)》 CAS 2009年第3期6-9,共4页
针对IEEE802.11e Medium Access Control层的QoS机制高负载时存在远端节点冲突和低优先级业务资源被耗尽的问题,提出在牺牲较小带宽的基础上增加一条忙音信道,取代CTS帧在数据信道上的广播,减少远端节点的冲突.仿真结果表明,该方案具有... 针对IEEE802.11e Medium Access Control层的QoS机制高负载时存在远端节点冲突和低优先级业务资源被耗尽的问题,提出在牺牲较小带宽的基础上增加一条忙音信道,取代CTS帧在数据信道上的广播,减少远端节点的冲突.仿真结果表明,该方案具有较小的冲突概率,有效地减少了远端节点冲突.同时提出一个解决公平性问题的新思路:在避退时间发送忙音抢占信道,以期提高低优先级业务的接入概率. 展开更多
关键词 IEEE 802.11e medium ACCESS CONTROL QOS机制 远端节点冲突 改进方案
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网络自出版平台的新变革——Medium 被引量:1
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作者 宋佳益 《科技传播》 2015年第13期80-81,共2页
作为网络自出版领域的优秀案例,Medium基于"乐于分享"的理念,成立三年来一直努力打造高效、精良的写作阅读平台。纵观Medium的发展历程与经营策略,笔者从以下三个方面论述了Medium为网络自出版平台带来的变革与创新:通过内容... 作为网络自出版领域的优秀案例,Medium基于"乐于分享"的理念,成立三年来一直努力打造高效、精良的写作阅读平台。纵观Medium的发展历程与经营策略,笔者从以下三个方面论述了Medium为网络自出版平台带来的变革与创新:通过内容聚合、反时间流与图文共生实现了内容管理方式的创新;关注总阅读时长这一测量指标,通过细化数据为平台改进提供优化方案;创新出版业商业模式——"平台"+"出版",同时聘请高素质写作团队保证内容的精英化倾向。 展开更多
关键词 medium 网络自出版平台 创新
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