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Antibody Titer Detection and Expression Characteristic Analysis of TypeⅡTomato Metacaspase Sl MC7 Protein in Fruit
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作者 Zhichao WANG Qiumin MA +3 位作者 Yanxiong YE shuai WEN Jingshun CAO Guiqin QU 《Agricultural Biotechnology》 CAS 2018年第2期10-14,共5页
SlMC7 prokaryotic expression recombinant protein was used as test materials. SlMC7 polyclonal antiserum was obtained from immunized mice by using purified SlMC7 recombinant protein from Escherichia coli. ELISA and pro... SlMC7 prokaryotic expression recombinant protein was used as test materials. SlMC7 polyclonal antiserum was obtained from immunized mice by using purified SlMC7 recombinant protein from Escherichia coli. ELISA and protein dot plot methods were used to detect the antibody titer and expression characteristics and the expression difference at different stages,providing material foundation and reference basis for subsequent SlMC7 functional research in tomato fruit. The results showed that the titer of SlMC7 polyclonal antibodies was more than 1∶ 256 000 and the sensitivity of SlMC7 polyclonal antibodies was 1. 6 ng at the dilutability of 1∶ 10 000. SlMC7 protein was detected at different stages,and SlMC7 had a higher expression level in color-break fruit,indicating that SlMC7 might be relevant to the fruit ripening of tomato. 展开更多
关键词 metacaspase Antibody protein expression TomatoHome
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