BACKGROUND Diabetic kidney disease(DKD),characterized by increased urinary microalbumin levels and decreased renal function,is the primary cause of end-stage renal di-sease.Its pathological mechanisms are complicated ...BACKGROUND Diabetic kidney disease(DKD),characterized by increased urinary microalbumin levels and decreased renal function,is the primary cause of end-stage renal di-sease.Its pathological mechanisms are complicated and multifactorial;Therefore,sensitive and specific biomarkers are needed.Urinary exosome originate from diverse renal cells in nephron segments and partially mirror the pathological changes in the kidney.The microRNAs(miRNAs)in urinary exosome are remark-ably stable and highly tissue-specific for the kidney.METHODS Type 2 diabetic mellitus(T2DM)patients were recruited from the Second Hospital of Hebei Medical University and were divided into two groups:DM,diabetic pa-tients without albuminuria[urinary albumin to creatinine ratio(UACR)<30 mg/g]and DKD,diabetic patients with albuminuria(UACR≥30 mg/g).Healthy subjects were the normal control(NC)group.Urinary exosomal miR-145-5p,miR-27a-3p,and miR-29c-3p,were detected using real-time quantitative polymerase chain reaction.The correlation between exosomal miRNAs and the clinical in-dexes was evaluated.The diagnostic values of exosomal miR-145-5p and miR-27a-3p in DKD were determined using receiver operating characteristic(ROC)analysis.Biological functions of miR-145-5p were investigated by performing RESULTS Urinary exosomal expression of miR-145-5p and miR-27a-3p was more upregulated in the DKD group than in the DM group(miR-145-5p:4.54±1.45 vs 1.95±0.93,P<0.001;miR-27a-3p:2.33±0.79 vs 1.71±0.76,P<0.05)and the NC group(miR-145-5p:4.54±1.45 vs 1.55±0.83,P<0.001;miR-27a-3p:2.33±0.79 vs 1.10±0.51,P<0.001).The exosomal miR-145-5p and miR-27a-3p positively correlated with albuminuria and serum creatinine and negatively correlated with the estimated glomerular filtration rate.miR-27a-3p was also closely related to blood glucose,gly-cosylated hemoglobin A1c,and low-density lipoprotein cholesterol.ROC analysis revealed that miR-145-5p had a better area under the curve of 0.88[95%confidence interval(CI):0.784-0.985,P<0.0001]in diagnosing DKD than miR-27a-3p with 0.71(95%CI:0.547-0.871,P=0.0239).Bioinformatics analysis revealed that the target genes of miR-145-5p were located in the actin filament,cytoskeleton,and extracellular exosome and were involved in the pathological processes of DKD,including apoptosis,inflammation,and fibrosis.CONCLUSION Urinary exosomal miR-145-5p and miR-27a-3p may serve as novel noninvasive diagnostic biomarkers or promising therapeutic targets for DKD.展开更多
目的探讨人微小RNA-27a(microRNA-27a,miR-27a)及其靶基因FOXO1在胃癌组织、癌旁组织中的变化及其在金雀异黄素抑制胃癌细胞增殖中的作用。方法利用Real Time PCR方法检测10对胃癌癌旁/癌组织中microRNA-27a及其靶基因FOXO1mRNA的表达...目的探讨人微小RNA-27a(microRNA-27a,miR-27a)及其靶基因FOXO1在胃癌组织、癌旁组织中的变化及其在金雀异黄素抑制胃癌细胞增殖中的作用。方法利用Real Time PCR方法检测10对胃癌癌旁/癌组织中microRNA-27a及其靶基因FOXO1mRNA的表达。不同浓度(5、10、20、40μmol.L-1)的金雀异黄素作用于胃癌细胞SGC-7901 72h后,用噻唑兰比色法(MTT)检测细胞增殖,并采用Real Time PCR方法检测细胞中miR-27a及其靶基因FOXO1mRNA的表达。结果胃癌组织较癌旁组织miR-27a表达升高(1.566±0.206 5),其靶基因FOXO1mRNA表达降低(0.298±0.156 6)。Pearson线性分析表明:miR-27a与FOXO1mRNA呈负相关(r=-0.701 8)。金雀异黄素作用于SGC-7901细胞后,细胞增殖得到了抑制,尤其在20与40μmol.L-1浓度下,细胞抑制率明显降低。不同浓度的金雀异黄素处理72h后,miR-27a表达与对照组比较明显降低;其靶基因FOXO1mRNA表达水平较对照组增加(均P<0.05)。结论金雀异黄素可能通过抑制miR-27a表达,促进FOXO1mR-NA的表达,进而抑制胃癌细胞增殖,发挥抗肿瘤的作用。展开更多
基金Supported by the Nature Science Foundation of Hebei Province,No.H2023104011.
文摘BACKGROUND Diabetic kidney disease(DKD),characterized by increased urinary microalbumin levels and decreased renal function,is the primary cause of end-stage renal di-sease.Its pathological mechanisms are complicated and multifactorial;Therefore,sensitive and specific biomarkers are needed.Urinary exosome originate from diverse renal cells in nephron segments and partially mirror the pathological changes in the kidney.The microRNAs(miRNAs)in urinary exosome are remark-ably stable and highly tissue-specific for the kidney.METHODS Type 2 diabetic mellitus(T2DM)patients were recruited from the Second Hospital of Hebei Medical University and were divided into two groups:DM,diabetic pa-tients without albuminuria[urinary albumin to creatinine ratio(UACR)<30 mg/g]and DKD,diabetic patients with albuminuria(UACR≥30 mg/g).Healthy subjects were the normal control(NC)group.Urinary exosomal miR-145-5p,miR-27a-3p,and miR-29c-3p,were detected using real-time quantitative polymerase chain reaction.The correlation between exosomal miRNAs and the clinical in-dexes was evaluated.The diagnostic values of exosomal miR-145-5p and miR-27a-3p in DKD were determined using receiver operating characteristic(ROC)analysis.Biological functions of miR-145-5p were investigated by performing RESULTS Urinary exosomal expression of miR-145-5p and miR-27a-3p was more upregulated in the DKD group than in the DM group(miR-145-5p:4.54±1.45 vs 1.95±0.93,P<0.001;miR-27a-3p:2.33±0.79 vs 1.71±0.76,P<0.05)and the NC group(miR-145-5p:4.54±1.45 vs 1.55±0.83,P<0.001;miR-27a-3p:2.33±0.79 vs 1.10±0.51,P<0.001).The exosomal miR-145-5p and miR-27a-3p positively correlated with albuminuria and serum creatinine and negatively correlated with the estimated glomerular filtration rate.miR-27a-3p was also closely related to blood glucose,gly-cosylated hemoglobin A1c,and low-density lipoprotein cholesterol.ROC analysis revealed that miR-145-5p had a better area under the curve of 0.88[95%confidence interval(CI):0.784-0.985,P<0.0001]in diagnosing DKD than miR-27a-3p with 0.71(95%CI:0.547-0.871,P=0.0239).Bioinformatics analysis revealed that the target genes of miR-145-5p were located in the actin filament,cytoskeleton,and extracellular exosome and were involved in the pathological processes of DKD,including apoptosis,inflammation,and fibrosis.CONCLUSION Urinary exosomal miR-145-5p and miR-27a-3p may serve as novel noninvasive diagnostic biomarkers or promising therapeutic targets for DKD.
文摘目的探讨人微小RNA-27a(microRNA-27a,miR-27a)及其靶基因FOXO1在胃癌组织、癌旁组织中的变化及其在金雀异黄素抑制胃癌细胞增殖中的作用。方法利用Real Time PCR方法检测10对胃癌癌旁/癌组织中microRNA-27a及其靶基因FOXO1mRNA的表达。不同浓度(5、10、20、40μmol.L-1)的金雀异黄素作用于胃癌细胞SGC-7901 72h后,用噻唑兰比色法(MTT)检测细胞增殖,并采用Real Time PCR方法检测细胞中miR-27a及其靶基因FOXO1mRNA的表达。结果胃癌组织较癌旁组织miR-27a表达升高(1.566±0.206 5),其靶基因FOXO1mRNA表达降低(0.298±0.156 6)。Pearson线性分析表明:miR-27a与FOXO1mRNA呈负相关(r=-0.701 8)。金雀异黄素作用于SGC-7901细胞后,细胞增殖得到了抑制,尤其在20与40μmol.L-1浓度下,细胞抑制率明显降低。不同浓度的金雀异黄素处理72h后,miR-27a表达与对照组比较明显降低;其靶基因FOXO1mRNA表达水平较对照组增加(均P<0.05)。结论金雀异黄素可能通过抑制miR-27a表达,促进FOXO1mR-NA的表达,进而抑制胃癌细胞增殖,发挥抗肿瘤的作用。
