[Objectives]This study was conducted to improve acidic soil and enhance the quality of tobacco leaves.[Methods]The effects of different microbial fertilizers on improving acidic tobacco-planting soil and tobacco leaf ...[Objectives]This study was conducted to improve acidic soil and enhance the quality of tobacco leaves.[Methods]The effects of different microbial fertilizers on improving acidic tobacco-planting soil and tobacco leaf quality were investigated through plot experiments.[Results]The application of microbial fertilizers could improve the pH value of acidic soil,and composite microbial agent A showed the best application effect.The application of bio-organic fertilizer was beneficial to improving the contents of available phosphorus and available boron in acidic soil.The application of composite microbial agent A was beneficial to improving the contents of available phosphorus and available potassium in acidic soil,and could promote the growth of tobacco plants and improve the economic traits of flue-cured tobacco and the coordination of chemical components in tobacco leaves.The application of composite microbial agent B led to a downward trend in the content of available boron in acidic soil.The application of composite microbial agent B could promote the absorption of nutrients by tobacco plants,and improve their disease resistance and the quality of tobacco leaves.Due to the differences in functional microorganisms contained,the application effects of different microbial fertilizers in improving acidic tobacco-planting soil and improving tobacco leaf quality varied.Overall,the application of microbial fertilizers could increase soil pH,activate soil nutrients,promote tobacco growth,enhance disease resistance,increase tobacco output value,and improve tobacco quality.Microbial fertilizers have good application prospects in improving acidic soil and improving tobacco quality.[Conclusions]The application of microbial fertilizers to improve acidic tobacco-planting soil can ensure the normal growth and development of tobacco plants and the improvement of tobacco leaf quality,achieving high-quality and sustainable development of Zhaotong tobacco.展开更多
Ecological prevention and control of plant disease is very important in sustainable agriculture.Adjusting soil p H value and fertilizing organic microbial fertilizer are two effective measures in this process.Kelp res...Ecological prevention and control of plant disease is very important in sustainable agriculture.Adjusting soil p H value and fertilizing organic microbial fertilizer are two effective measures in this process.Kelp residue contains a large amount of organic compounds and alkaline metal ions.The bio-control Bacillus amyloliquefaciens strain Hitwh-BA2 was inoculated into kelp residue medium to produce kelp residue microbial fertilizer.Acidic soil and alkaline soil were used to study the effect of kelp residue microbial fertilizer on soil p H and soil suppressive activity.Tip-culture method was used to determine soil leachate suppressive activity,which characterized the soil suppressive activity.Results showed that fertilizing kelp residue microbial fertilizer had increased the soil p H and soil suppressive ability significantly,which was verified by peanut validation experiments as well.Peanut potting experiments proved that fertilizing kelp residue microbial fertilizer not only improved the yield of peanuts obviously,but also reduced the amount of Aspergillus parasiticus 95 in peanut geocarposphere soil significantly.Results also showed that fertilizing kelp residue microbial fertilizer was effective in reducing A.parasiticus 95 infection rate.So the kelp residue microbial fertilizer has good potential application prospect on ecological prevention and control of plant disease.展开更多
Root-knot nematodes(RKNs) cause huge yield losses to agricultural crops worldwide. Meanwhile, livestock manure is often improperly managed by farmers, which leads to serious environmental pollution. To resolve these t...Root-knot nematodes(RKNs) cause huge yield losses to agricultural crops worldwide. Meanwhile, livestock manure is often improperly managed by farmers, which leads to serious environmental pollution. To resolve these two problems, this study developed a procedure for the conversion of chicken manure to organic fertilizer by larvae of Hermetia illucens L. and Bacillus subtilis BSF-CL. Chicken manure organic fertilizer was then mixed thoroughly with Paenibacillus polymyxa KM2501-1 to a final concentration of 1.5×10^(8)CFU g^(-1). The efficacy of KM2501-1 microbial organic fertilizer in controlling root-knot nematodes was evaluated in pot and field experiments. In pot experiments, applying KM2501-1 microbial organic fertilizer either as a base fertilizer or as a fumigant at the dose of 40 g/pot suppressed root-knot disease by 61.76 and 69.05% compared to the corresponding control treatments, respectively. When applied as a fumigant at the dose of 1 kg m;in field experiments, KM2501-1 microbial organic fertilizer enhanced the growth of tomato plants, suppressed root-knot disease by 49.97%, and reduced second stage juveniles of RKN in soil by 88.68%. KM2501-1 microbial organic fertilizer controlled RKNs better than commercial bio-organic fertilizer in both pot and field experiments. These results demonstrate that this co-conversion process efficiently transforms chicken manure into high value-added larvae biomass and KM2501-1 microbial organic fertilizer with potential application as a novel nematode control agent.展开更多
The experiment was conducted to study the impact of application of microbial inoculants, compared with no microbial fertilizer, on enzyme activity, microbial biomass and available nutrient contents in paddy soil in He...The experiment was conducted to study the impact of application of microbial inoculants, compared with no microbial fertilizer, on enzyme activity, microbial biomass and available nutrient contents in paddy soil in Heilongjiang Province. The application of soil phosphorus activator was able to increase the quantity of bacteria and fungi in soil, but its effect on actinomycetes in soil was not significant. The application of microbial inoculants increased the urease and sucrase activities in soil over the growing season, but only at the maturing stage soil acid phosphatase activity was enhanced with the applying soil phosphorus activator. The application of soil phosphorus activator increased alkali-hydrolyzable nitrogen and available phosphorus contents in soil, but did not increase available potassium content in soil. The optimal microbial inoculant application rate as applied as soil phosphorus activator was 7.5 kg·hm^(-2).展开更多
[Objectives]To investigate the application effect of compound microbial fertilizer on crops.[Methods]Livestock and poultry breeding waste,rapeseed cake and peanut straw were fully decomposed,and then added with compou...[Objectives]To investigate the application effect of compound microbial fertilizer on crops.[Methods]Livestock and poultry breeding waste,rapeseed cake and peanut straw were fully decomposed,and then added with compound functional microbial inoculum to produce Aisi Si/TE(active chelated silicon/trace element)compound microbial fertilizer,which was used to conduct 10%nitrogen reduction alternative fertilization experiment on rice.[Results]The yield of rice applied with 225 kg/ha Aisi Si/TE compound bacterial fertilizer was 7203 kg/ha,increased by 5.4%,6.9%and 46.9%,respectively compared to those of rice applied with 225 kg/ha inactivated Aisi Si/TE compound microbial fertilizer,conventional fertilization and blank control(P<0.01).Application of Aisi Si/TE compound microbial fertilizer to rice improved soil organic matter and effective nutrient content and showed obvious effect of saving nitrogen and increasing yield and income.In addition,it provided a good micro-ecological environment,passivated and solidified heavy metals,effectively reduced the biological mobility of heavy metals,and greatly reduced the cadmium content in rice.[Conclusions]Application of Aisi Si/TE compound microbial fertilizer is beneficial to improving the quality of agricultural products.展开更多
With the improvement of living standards,people pay more and more attention to the quality and safety of rice.Microbial agents are favored by the public because they can activate the nutrient supply in the soil,and re...With the improvement of living standards,people pay more and more attention to the quality and safety of rice.Microbial agents are favored by the public because they can activate the nutrient supply in the soil,and reduce the residue and application amount of chemical fertilizers and pesticides.Based on the conventional fertilization in the field,Bacillus mucilaginosus and Aspergillus niger were applied,Bacillus mucilaginosus was inoculated at four levels in the paddy soil in the cold region of Heilongjiang Province of China.The effects of different proportions of Bacillus mucilaginosus and Aspergillus niger on the number of soil microorganisms,enzyme activity,microbial biomass,soil biochemical intensity,soil nutrient content,plant nutrient content and yield were studied,and the effects on the plant nutrient content of rice and the nutrient dynamics were discussed.The results showed that a 2.62%-21.20%higher yield of rice obtained from co-application treatments compared with that of the control-blank treatment.Furthermore,the highest yield obtained(10736±65 kg/hm^(2))suggested that the optimized values for the two bacteria applied were 120×10^(11) CFU/hm^(2) for Bacillus mucilaginosus and 15×10^(11) CFU/hm^(2) for Aspergillus niger.Bacillus mucilaginosus can decompose minerals in soil,dissolve potassium and silicon,decompose apatite and release phosphorus into soil.Aspergillus niger can transform the phosphate which cannot be absorbed by plants into soluble phosphate which can be directly absorbed by plants by producing non-volatile acids.In particular,Bacillus mucilaginosus and Aspergillus niger have synergistic effect,and their combined application effect is greater than that of two bacteria alone.Co-application promoted the release of soil soluble silicon,and then increased the silicon content of plants.At the same time,soil microorganism,microbial biomass,enzyme activity and biochemical activity all increased significantly.This study provides an effective way to reduce the amount of chemical fertilizer applied in rice production in cold regions of China.展开更多
基金Supported by Science and Technology Program Major Project of Yunnan Branch,China National Tobacco Corporation(2020530000241022)。
文摘[Objectives]This study was conducted to improve acidic soil and enhance the quality of tobacco leaves.[Methods]The effects of different microbial fertilizers on improving acidic tobacco-planting soil and tobacco leaf quality were investigated through plot experiments.[Results]The application of microbial fertilizers could improve the pH value of acidic soil,and composite microbial agent A showed the best application effect.The application of bio-organic fertilizer was beneficial to improving the contents of available phosphorus and available boron in acidic soil.The application of composite microbial agent A was beneficial to improving the contents of available phosphorus and available potassium in acidic soil,and could promote the growth of tobacco plants and improve the economic traits of flue-cured tobacco and the coordination of chemical components in tobacco leaves.The application of composite microbial agent B led to a downward trend in the content of available boron in acidic soil.The application of composite microbial agent B could promote the absorption of nutrients by tobacco plants,and improve their disease resistance and the quality of tobacco leaves.Due to the differences in functional microorganisms contained,the application effects of different microbial fertilizers in improving acidic tobacco-planting soil and improving tobacco leaf quality varied.Overall,the application of microbial fertilizers could increase soil pH,activate soil nutrients,promote tobacco growth,enhance disease resistance,increase tobacco output value,and improve tobacco quality.Microbial fertilizers have good application prospects in improving acidic soil and improving tobacco quality.[Conclusions]The application of microbial fertilizers to improve acidic tobacco-planting soil can ensure the normal growth and development of tobacco plants and the improvement of tobacco leaf quality,achieving high-quality and sustainable development of Zhaotong tobacco.
基金Sponsored by the National Natural Science Foundation of China(Grant Nos.30571244 and 30870003)China National Key Technology R&D Program(Grant No.2009BADA0B05-4)
文摘Ecological prevention and control of plant disease is very important in sustainable agriculture.Adjusting soil p H value and fertilizing organic microbial fertilizer are two effective measures in this process.Kelp residue contains a large amount of organic compounds and alkaline metal ions.The bio-control Bacillus amyloliquefaciens strain Hitwh-BA2 was inoculated into kelp residue medium to produce kelp residue microbial fertilizer.Acidic soil and alkaline soil were used to study the effect of kelp residue microbial fertilizer on soil p H and soil suppressive activity.Tip-culture method was used to determine soil leachate suppressive activity,which characterized the soil suppressive activity.Results showed that fertilizing kelp residue microbial fertilizer had increased the soil p H and soil suppressive ability significantly,which was verified by peanut validation experiments as well.Peanut potting experiments proved that fertilizing kelp residue microbial fertilizer not only improved the yield of peanuts obviously,but also reduced the amount of Aspergillus parasiticus 95 in peanut geocarposphere soil significantly.Results also showed that fertilizing kelp residue microbial fertilizer was effective in reducing A.parasiticus 95 infection rate.So the kelp residue microbial fertilizer has good potential application prospect on ecological prevention and control of plant disease.
基金supported by the project from China Ocean Mineral Resources R&D Association(COMRADY135-B2-17)+1 种基金the National Key Technologies R&D Program of China(2018YFD0500203 and 2018YFF0213503)the Major Projects of Technological Innovation in Hubei Province,China(2017ABA072)。
文摘Root-knot nematodes(RKNs) cause huge yield losses to agricultural crops worldwide. Meanwhile, livestock manure is often improperly managed by farmers, which leads to serious environmental pollution. To resolve these two problems, this study developed a procedure for the conversion of chicken manure to organic fertilizer by larvae of Hermetia illucens L. and Bacillus subtilis BSF-CL. Chicken manure organic fertilizer was then mixed thoroughly with Paenibacillus polymyxa KM2501-1 to a final concentration of 1.5×10^(8)CFU g^(-1). The efficacy of KM2501-1 microbial organic fertilizer in controlling root-knot nematodes was evaluated in pot and field experiments. In pot experiments, applying KM2501-1 microbial organic fertilizer either as a base fertilizer or as a fumigant at the dose of 40 g/pot suppressed root-knot disease by 61.76 and 69.05% compared to the corresponding control treatments, respectively. When applied as a fumigant at the dose of 1 kg m;in field experiments, KM2501-1 microbial organic fertilizer enhanced the growth of tomato plants, suppressed root-knot disease by 49.97%, and reduced second stage juveniles of RKN in soil by 88.68%. KM2501-1 microbial organic fertilizer controlled RKNs better than commercial bio-organic fertilizer in both pot and field experiments. These results demonstrate that this co-conversion process efficiently transforms chicken manure into high value-added larvae biomass and KM2501-1 microbial organic fertilizer with potential application as a novel nematode control agent.
文摘The experiment was conducted to study the impact of application of microbial inoculants, compared with no microbial fertilizer, on enzyme activity, microbial biomass and available nutrient contents in paddy soil in Heilongjiang Province. The application of soil phosphorus activator was able to increase the quantity of bacteria and fungi in soil, but its effect on actinomycetes in soil was not significant. The application of microbial inoculants increased the urease and sucrase activities in soil over the growing season, but only at the maturing stage soil acid phosphatase activity was enhanced with the applying soil phosphorus activator. The application of soil phosphorus activator increased alkali-hydrolyzable nitrogen and available phosphorus contents in soil, but did not increase available potassium content in soil. The optimal microbial inoculant application rate as applied as soil phosphorus activator was 7.5 kg·hm^(-2).
文摘[Objectives]To investigate the application effect of compound microbial fertilizer on crops.[Methods]Livestock and poultry breeding waste,rapeseed cake and peanut straw were fully decomposed,and then added with compound functional microbial inoculum to produce Aisi Si/TE(active chelated silicon/trace element)compound microbial fertilizer,which was used to conduct 10%nitrogen reduction alternative fertilization experiment on rice.[Results]The yield of rice applied with 225 kg/ha Aisi Si/TE compound bacterial fertilizer was 7203 kg/ha,increased by 5.4%,6.9%and 46.9%,respectively compared to those of rice applied with 225 kg/ha inactivated Aisi Si/TE compound microbial fertilizer,conventional fertilization and blank control(P<0.01).Application of Aisi Si/TE compound microbial fertilizer to rice improved soil organic matter and effective nutrient content and showed obvious effect of saving nitrogen and increasing yield and income.In addition,it provided a good micro-ecological environment,passivated and solidified heavy metals,effectively reduced the biological mobility of heavy metals,and greatly reduced the cadmium content in rice.[Conclusions]Application of Aisi Si/TE compound microbial fertilizer is beneficial to improving the quality of agricultural products.
基金This work was supported by Research Science Foundation in Technology Innovation of Harbin(Grant No.RC2013XK002015).
文摘With the improvement of living standards,people pay more and more attention to the quality and safety of rice.Microbial agents are favored by the public because they can activate the nutrient supply in the soil,and reduce the residue and application amount of chemical fertilizers and pesticides.Based on the conventional fertilization in the field,Bacillus mucilaginosus and Aspergillus niger were applied,Bacillus mucilaginosus was inoculated at four levels in the paddy soil in the cold region of Heilongjiang Province of China.The effects of different proportions of Bacillus mucilaginosus and Aspergillus niger on the number of soil microorganisms,enzyme activity,microbial biomass,soil biochemical intensity,soil nutrient content,plant nutrient content and yield were studied,and the effects on the plant nutrient content of rice and the nutrient dynamics were discussed.The results showed that a 2.62%-21.20%higher yield of rice obtained from co-application treatments compared with that of the control-blank treatment.Furthermore,the highest yield obtained(10736±65 kg/hm^(2))suggested that the optimized values for the two bacteria applied were 120×10^(11) CFU/hm^(2) for Bacillus mucilaginosus and 15×10^(11) CFU/hm^(2) for Aspergillus niger.Bacillus mucilaginosus can decompose minerals in soil,dissolve potassium and silicon,decompose apatite and release phosphorus into soil.Aspergillus niger can transform the phosphate which cannot be absorbed by plants into soluble phosphate which can be directly absorbed by plants by producing non-volatile acids.In particular,Bacillus mucilaginosus and Aspergillus niger have synergistic effect,and their combined application effect is greater than that of two bacteria alone.Co-application promoted the release of soil soluble silicon,and then increased the silicon content of plants.At the same time,soil microorganism,microbial biomass,enzyme activity and biochemical activity all increased significantly.This study provides an effective way to reduce the amount of chemical fertilizer applied in rice production in cold regions of China.
