Background The provision of environmental enrichments to Muscovy ducks could reduce the expression of the aggressive behaviors.The aim of the present study was to evaluate the effects of black soldier fly(BSF)and yell...Background The provision of environmental enrichments to Muscovy ducks could reduce the expression of the aggressive behaviors.The aim of the present study was to evaluate the effects of black soldier fly(BSF)and yellow mealworm(YM)live larva provision on Muscovy duck performance,excreta corticosterone metabolites(ECM),behavior,and blood parameters.Methods A total of 1263-day-old female Muscovy ducklings were allotted to 18 pens(6 replicates/treatment,7 birds/pen)and assigned to 3 experimental treatments:a control group fed commercial feed,and two experimental treatments fed commercial feed plus the 5%(based on the expected daily feed intake,as fed basis)of BSF and YM live larvae(BSF and YM groups,respectively).A two-phase feeding program was applied:starter(from 3 to 31 days of age)and grower-finisher(from 32 to 55 days of age).The live weight,average daily gain,average daily feed intake,and feed conversion ratio were calculated.Larva consumption times were collected,and video recordings were performed during 3 periods(P)each day:the hour before(P1),during(P2),and after(P3)the larva administration.ECM were evaluated at 3,31,and 55-day-old.Finally,the total red and white blood cell counts,serum proteins,lipids,and liver and renal function serum enzymes were evaluated on 12 birds/treatment.Results The experimental treatment did not affect the growth performance of the birds(P>0.05).Larva consumption times were always similar between the two insect species,except at 14–18 days of age,were BSF larvae were consumed faster than YM larvae(P<0.001).The birds showed less walking activity during P2,and preening behavior increased in YM birds during P3.The C birds increased the attack behavior over the weeks(P<0.05).During weeks 1–3 the YM group reduced the attack frequency(P1>P3;P<0.05).Finally,the provision of live BSF and YM larvae significantly reduced the ECM at 55 days of age and the heterophil to lymphocyte ratio(P<0.05).Conclusions Live BSF and YM larva supplementation in Muscovy duck improves duck welfare,without impairing birds'growth performance.展开更多
Background:Aquaculture must continue to reduce dependence on fishmeal(FM)and fishoil in feeds to ensure sustainable sector growth.Therefore,the use of novel aquaculture feed ingredients is growing.In this regard,insec...Background:Aquaculture must continue to reduce dependence on fishmeal(FM)and fishoil in feeds to ensure sustainable sector growth.Therefore,the use of novel aquaculture feed ingredients is growing.In this regard,insects can represent a new world of sustainable and protein-rich ingredients for farmed fish feeds.Accordingly,we investigated the effects of full replacement of FM with Tenebrio molitor(TM)larvae meal in the diet of rainbow trout(Oncorhynchus mykiss)on fish gut and skin microbiota.Methods:A feeding trial was conducted with 126 trout of about 80 g mean initial weight that were fed for 22 weeks with two isonitrogenous,isolipidic,and isoenergetic extruded experimental diets.Partially defatted TM meal was included in one of the diets to replace 100%(TM 100)of FM,whereas the other diet(TM 0)was without TM.To analyse the microbial communities,the Illumina MiSeq platform for sequencing of 16S rRNA gene and Qiime pipeline were used to identify bacteria in the gut and skin mucosa,and in the diets.Results:The data showed no major effects of full FM substitution with TM meal on bacterial species richness and diversity in both,gut mucosa-and skin mucus-associated microbiome.Skin microbiome was dominated by phylum Proteobacteria and especially by Gammaproteobacteria class that constituted approximately half of the bacterial taxa found.The two dietary fish groups did not display distinctive features,except for a decrease in the relative abundance of Deefgea genus(family Neisseriaceae)in trout fed with insect meal.The metagenomic analysis of the gut mucosa indicated that Tenericutes was the most abundant phylum,regardless of the diet.Specifically,within this phylum,the Mollicutes,mainly represented by Mycoplasmataceae family,were the dominant class.However,we observed only a weak dietary modulation of intestinal bacterial communities.The only changes due to full FM replacement with TM meal were a decreased number of Proteobacteria and a reduced number of taxa assigned to Ruminococcaceae and Neisseriaceae families.Conclusions:The data demonstrated that TM larvae meal is a valid alternative animal protein to replace FM in the aquafeeds.Only slight gut and skin microbiota changes occurred in rainbow trout after total FM replacement with insect meal.The mapping of the trout skin microbiota represents a novel contribution of the present study.Indeed,in contrast to the increasing knowledge on gut microbiota,the skin microbiota of major farmed fish species remains largely unmapped but it deserves thorough consideration.展开更多
In order to reduce the economic costs for breeding Tenebrio molitor L. and increase the utilization rate of corn stalk,with corn,wheat bran and corn stalk as the raw materials,13 different compound feed were designed ...In order to reduce the economic costs for breeding Tenebrio molitor L. and increase the utilization rate of corn stalk,with corn,wheat bran and corn stalk as the raw materials,13 different compound feed were designed by Minitab( R) 15. 1. 0. 0. The design was then divided into two groups based on addition or without addition of vegetable leaves to study the effects of compound feed and vegetable leaves on growth and development of T. molitor. The results showed that body weight,duration,crude protein content and pupation rate of larvae and emergence rate of adults were significantly affected by compound feed and vegetable leaves( P <0. 05). The larvae bred with feed 5 had the largest body weight,with the shortest duration; the larvae bred with feed 3 had the highest pupation rate; the larvae bred with feed 7 had the highest crude protein content;the adult bred with feed 9 had the largest emergence rate. Overall breeding effect of feed 7 + vegetable leaves was better than any other groups,with ideal indicators,higher utilization rate and lower breeding cost. Its formula was w( wheat bran) = 33. 33%,w( corn) = 33. 33%,w( corn stalks) = 33. 33%. Therefore,the growth of T. molitor was significantly affected by feed types,and feed 7 + vegetable leaves could be promoted in the production.展开更多
For centuries, insects have been used as food due to their availability and easiness in raising that is much less burdensome for environment than animal husbandry breeding. Mealworm (Tenebrio molitor L.) is a store-pe...For centuries, insects have been used as food due to their availability and easiness in raising that is much less burdensome for environment than animal husbandry breeding. Mealworm (Tenebrio molitor L.) is a store-pest of which larvae are consumed by people. The aim of the work was to determine the nutritional value of larvae of mealworm (Tenebrio molitor L.). The material was a three-month-old mealworm larva 25 -30 mmin length. Larvae were boiled for 3 min and next dried in 60℃. Contents of water, ash, minerals, protein, fat and fat acids profile have been determined. Fresh larvae contained 56% of water, 18% of total protein, 22% of total fat and 1.55% of ash. High contents of minerals were found in the larvae: magnesium (87.5 mg/100g), zinc (4.2 mg/100g), iron (3.8 mg/100g), copper (0.78 mg/100g) and manganese (0.44 mg/100g). The proportion of n-6/n-3 fatty acids was very advantageous and amounted to 6.76. Larvae powder contained twice higher content of protein, fat, ash and minerals. Larva of mealworm is a valuable source of nutrients in amounts more profitable for human organism than traditional meat food. Powdered larva is a high-grade product to be applied as a supplement to traditional meals.展开更多
Males and females often differ in their susceptibility and exposure to infection. Thus, they may also differ in their ability to avoid infection. The beetle, Tenebrio molitor, risks infection with cysticercoids when i...Males and females often differ in their susceptibility and exposure to infection. Thus, they may also differ in their ability to avoid infection. The beetle, Tenebrio molitor, risks infection with cysticercoids when ingesting rat feces containing eggs of the tapeworm, Hymenolepis diminuta. Previous studies demonstrated that beetles sometimes prefer infective feces suggesting that the tapeworm influences the foraging behavior of beetles. After recording beetle movement in an arena containing infective and uninfective feces for one hour, sex main effects were not significant, but the effect of sex asked in trials was significant for males. Specifically, more male beetles occurred on the uninfective bait than on the infective bait. This observation suggests that male beetles avoid infective feces, which decreases their probability of infection with H. diminuta. If the cost of infection is higher in males, then selection to avoid infective feces may act more strongly on males.展开更多
[Objective] To respectively compare esterase isozymes and peroxidase isozymes among black worm Tenebrio molitor Linnaeus (Tenebrio molitor L.) at different developmental stages and thus provide a theoretical basis for...[Objective] To respectively compare esterase isozymes and peroxidase isozymes among black worm Tenebrio molitor Linnaeus (Tenebrio molitor L.) at different developmental stages and thus provide a theoretical basis for species identification and large-scale farming. [Method] The esterase isozymes and peroxidase isozymes of black worm Tenebrio molitor L. at different developmental stages were respectively analyzed by polyacrylamide gel electrophoresis. [Result] Both esterase isozymes and peroxidase isozymes were different among black worm Tenebrio molitor L. at developmental stages, larvae in different instars or between the male adults and the female adults. [Conclusion] The esterase isozymes, peroxidase isozymes and physiological characteristics of black worm Tenebrio molitor L. varies with developmental stages.展开更多
基金supported by the PRIMA programme under grant agreement No 2015,project SUSTAvian FEEDsupported by the European Unionthe visit by Achille Schiavone(22216/IV/23-Poultry MED project)funded by the Fundación Séneca-Agencia de Ciencia y Tecnología de la Región de Murcia in connection with the“Jiménez De La Espada”Regional Programme for Mobility,Collaboration and Knowledge Exchange。
文摘Background The provision of environmental enrichments to Muscovy ducks could reduce the expression of the aggressive behaviors.The aim of the present study was to evaluate the effects of black soldier fly(BSF)and yellow mealworm(YM)live larva provision on Muscovy duck performance,excreta corticosterone metabolites(ECM),behavior,and blood parameters.Methods A total of 1263-day-old female Muscovy ducklings were allotted to 18 pens(6 replicates/treatment,7 birds/pen)and assigned to 3 experimental treatments:a control group fed commercial feed,and two experimental treatments fed commercial feed plus the 5%(based on the expected daily feed intake,as fed basis)of BSF and YM live larvae(BSF and YM groups,respectively).A two-phase feeding program was applied:starter(from 3 to 31 days of age)and grower-finisher(from 32 to 55 days of age).The live weight,average daily gain,average daily feed intake,and feed conversion ratio were calculated.Larva consumption times were collected,and video recordings were performed during 3 periods(P)each day:the hour before(P1),during(P2),and after(P3)the larva administration.ECM were evaluated at 3,31,and 55-day-old.Finally,the total red and white blood cell counts,serum proteins,lipids,and liver and renal function serum enzymes were evaluated on 12 birds/treatment.Results The experimental treatment did not affect the growth performance of the birds(P>0.05).Larva consumption times were always similar between the two insect species,except at 14–18 days of age,were BSF larvae were consumed faster than YM larvae(P<0.001).The birds showed less walking activity during P2,and preening behavior increased in YM birds during P3.The C birds increased the attack behavior over the weeks(P<0.05).During weeks 1–3 the YM group reduced the attack frequency(P1>P3;P<0.05).Finally,the provision of live BSF and YM larvae significantly reduced the ECM at 55 days of age and the heterophil to lymphocyte ratio(P<0.05).Conclusions Live BSF and YM larva supplementation in Muscovy duck improves duck welfare,without impairing birds'growth performance.
基金This research was partially funded by AGER,Network Foundation,Project Fine Feed for Fish(4F)Rif.No.2016-01-01This work was also co-funded by the EU Horizon 2020 AquaIMPACT(Genomic and nutritional innovations for genetically superior farmed fish to improve efficiency in European aquaculture),number:818367.
文摘Background:Aquaculture must continue to reduce dependence on fishmeal(FM)and fishoil in feeds to ensure sustainable sector growth.Therefore,the use of novel aquaculture feed ingredients is growing.In this regard,insects can represent a new world of sustainable and protein-rich ingredients for farmed fish feeds.Accordingly,we investigated the effects of full replacement of FM with Tenebrio molitor(TM)larvae meal in the diet of rainbow trout(Oncorhynchus mykiss)on fish gut and skin microbiota.Methods:A feeding trial was conducted with 126 trout of about 80 g mean initial weight that were fed for 22 weeks with two isonitrogenous,isolipidic,and isoenergetic extruded experimental diets.Partially defatted TM meal was included in one of the diets to replace 100%(TM 100)of FM,whereas the other diet(TM 0)was without TM.To analyse the microbial communities,the Illumina MiSeq platform for sequencing of 16S rRNA gene and Qiime pipeline were used to identify bacteria in the gut and skin mucosa,and in the diets.Results:The data showed no major effects of full FM substitution with TM meal on bacterial species richness and diversity in both,gut mucosa-and skin mucus-associated microbiome.Skin microbiome was dominated by phylum Proteobacteria and especially by Gammaproteobacteria class that constituted approximately half of the bacterial taxa found.The two dietary fish groups did not display distinctive features,except for a decrease in the relative abundance of Deefgea genus(family Neisseriaceae)in trout fed with insect meal.The metagenomic analysis of the gut mucosa indicated that Tenericutes was the most abundant phylum,regardless of the diet.Specifically,within this phylum,the Mollicutes,mainly represented by Mycoplasmataceae family,were the dominant class.However,we observed only a weak dietary modulation of intestinal bacterial communities.The only changes due to full FM replacement with TM meal were a decreased number of Proteobacteria and a reduced number of taxa assigned to Ruminococcaceae and Neisseriaceae families.Conclusions:The data demonstrated that TM larvae meal is a valid alternative animal protein to replace FM in the aquafeeds.Only slight gut and skin microbiota changes occurred in rainbow trout after total FM replacement with insect meal.The mapping of the trout skin microbiota represents a novel contribution of the present study.Indeed,in contrast to the increasing knowledge on gut microbiota,the skin microbiota of major farmed fish species remains largely unmapped but it deserves thorough consideration.
基金Supported by Agricultural Research Project of Shaanxi Provincial Science and Technology Department(2012.k02-10)Innovation and Entrepreneurship Training Project of University Students in Shaanxi Province(1252)Special Funds of Ecology for Construction of High-level University in Shaanxi Province(2012SXTS03)
文摘In order to reduce the economic costs for breeding Tenebrio molitor L. and increase the utilization rate of corn stalk,with corn,wheat bran and corn stalk as the raw materials,13 different compound feed were designed by Minitab( R) 15. 1. 0. 0. The design was then divided into two groups based on addition or without addition of vegetable leaves to study the effects of compound feed and vegetable leaves on growth and development of T. molitor. The results showed that body weight,duration,crude protein content and pupation rate of larvae and emergence rate of adults were significantly affected by compound feed and vegetable leaves( P <0. 05). The larvae bred with feed 5 had the largest body weight,with the shortest duration; the larvae bred with feed 3 had the highest pupation rate; the larvae bred with feed 7 had the highest crude protein content;the adult bred with feed 9 had the largest emergence rate. Overall breeding effect of feed 7 + vegetable leaves was better than any other groups,with ideal indicators,higher utilization rate and lower breeding cost. Its formula was w( wheat bran) = 33. 33%,w( corn) = 33. 33%,w( corn stalks) = 33. 33%. Therefore,the growth of T. molitor was significantly affected by feed types,and feed 7 + vegetable leaves could be promoted in the production.
文摘For centuries, insects have been used as food due to their availability and easiness in raising that is much less burdensome for environment than animal husbandry breeding. Mealworm (Tenebrio molitor L.) is a store-pest of which larvae are consumed by people. The aim of the work was to determine the nutritional value of larvae of mealworm (Tenebrio molitor L.). The material was a three-month-old mealworm larva 25 -30 mmin length. Larvae were boiled for 3 min and next dried in 60℃. Contents of water, ash, minerals, protein, fat and fat acids profile have been determined. Fresh larvae contained 56% of water, 18% of total protein, 22% of total fat and 1.55% of ash. High contents of minerals were found in the larvae: magnesium (87.5 mg/100g), zinc (4.2 mg/100g), iron (3.8 mg/100g), copper (0.78 mg/100g) and manganese (0.44 mg/100g). The proportion of n-6/n-3 fatty acids was very advantageous and amounted to 6.76. Larvae powder contained twice higher content of protein, fat, ash and minerals. Larva of mealworm is a valuable source of nutrients in amounts more profitable for human organism than traditional meat food. Powdered larva is a high-grade product to be applied as a supplement to traditional meals.
文摘Males and females often differ in their susceptibility and exposure to infection. Thus, they may also differ in their ability to avoid infection. The beetle, Tenebrio molitor, risks infection with cysticercoids when ingesting rat feces containing eggs of the tapeworm, Hymenolepis diminuta. Previous studies demonstrated that beetles sometimes prefer infective feces suggesting that the tapeworm influences the foraging behavior of beetles. After recording beetle movement in an arena containing infective and uninfective feces for one hour, sex main effects were not significant, but the effect of sex asked in trials was significant for males. Specifically, more male beetles occurred on the uninfective bait than on the infective bait. This observation suggests that male beetles avoid infective feces, which decreases their probability of infection with H. diminuta. If the cost of infection is higher in males, then selection to avoid infective feces may act more strongly on males.
基金supported by Hebei Education Commission Project (2003308) Qinhuangdao Science and Technology Commission Project
文摘[Objective] To respectively compare esterase isozymes and peroxidase isozymes among black worm Tenebrio molitor Linnaeus (Tenebrio molitor L.) at different developmental stages and thus provide a theoretical basis for species identification and large-scale farming. [Method] The esterase isozymes and peroxidase isozymes of black worm Tenebrio molitor L. at different developmental stages were respectively analyzed by polyacrylamide gel electrophoresis. [Result] Both esterase isozymes and peroxidase isozymes were different among black worm Tenebrio molitor L. at developmental stages, larvae in different instars or between the male adults and the female adults. [Conclusion] The esterase isozymes, peroxidase isozymes and physiological characteristics of black worm Tenebrio molitor L. varies with developmental stages.
