Background:Short term monocular deprivation allows for the modulation of ocular dominance,such that the previously deprived eye contribution will increase,while that of non-deprived eye will decrease.This study examin...Background:Short term monocular deprivation allows for the modulation of ocular dominance,such that the previously deprived eye contribution will increase,while that of non-deprived eye will decrease.This study examines the effects of short monocular occlusion on the Pulfrich phenomenon,an illusory perception of a horizontally moving object moving in an elliptical orbit in depth.In addition,we will explore whether the modulation of the Pulfrich effect is produced in the magnocellular pathway or the parvocellular pathway,by comparing two protocols,each designed to activate one pathway at a time.Methods:The stimulus used throughout the experiment is made up of elements defining a cylinder rotating in depth,allowing to measure interocular delay.The task consists of reporting the direction of rotation of the stimulus presented.There are two different stimuli:the P stimulus is composed of small elements oscillating slowly,which stimulates the parvocellular pathway,and the M stimulus which is composed of large elements oscillating rapidly which stimulates the magnocellular pathway.One experimental session consists of pre-patch testing,one hour of patching,and a post-patch testing.Each participant performs four sessions,both stimuli for each eye.Results:The point of subjective equivalence(PSE)is extracted from psychometric functions obtained during pre-testing and post-testing.Following deprivation of the left eye the PSE shifts negatively,whereas deprivation of the right eye shifts the PSE positively on the psychometric function.This indicated that monocular deprivation slows the perceptual processes of the previously patched eye.The amplitude of this effect is larger for the M protocol than it is for the P protocol.Conclusions:Contrary to expectations,results showed that effects of monocular deprivation are not exclusively mediated by contrast gain mechanisms,as suggested by Zhou and colleagues(2014).The amplitude of the differences observed for the M protocole suggest that the plasticity induced by short term deprivation is equally subjected to dynamic components.展开更多
Background:Short-term monocular deprivation has been recently shown to temporarily increase the sensitivity of the patched eye.Many studies have patched subjects for an arbitrary period of 2.5 hours,but for no princip...Background:Short-term monocular deprivation has been recently shown to temporarily increase the sensitivity of the patched eye.Many studies have patched subjects for an arbitrary period of 2.5 hours,but for no principled reason.Our goal is to show a relationship,if any,between the length of patching duration and the strength of its effect.Methods:We tested nine subjects with three different patching durations:1-,2-,3-hour.Four of the nine subjects were patched for 5-hour.Monocular deprivation was achieved by the use of a translucent eyepatch.A session included two rounds of baseline testing of interocular eye balance,patching,and post-patching tests.Each post-patching test occurred at 0,3,6,12,24,48,60 and 96 minutes after patching to track the patching effect over time.Every subject performed two sessions per condition.Results:One-hour patching produced a small shift in ocular dominance.A larger shift occurred from 2-hour patching,but 3-hour patching produced a comparable effect to the one measured after 2-hour patching.Conclusions:These results show a saturation of the patching effect beyond 2-hour patching.Hence,we believe that 2-hour patching duration is the optimal duration for eye dominance changes induced by monocular deprivation.展开更多
Background:We investigate whether changes in visual plasticity induced by monocular deprivation can be maintained across multiple days.It has been known that monocular deprivation strengthens the deprived eye in adult...Background:We investigate whether changes in visual plasticity induced by monocular deprivation can be maintained across multiple days.It has been known that monocular deprivation strengthens the deprived eye in adults with normal vision for a short period of time(30-60 minutes).This has been shown through a variety of visual tasks such as binocular combination and rivalry.Methods:Ten subjects were recruited and patched for five consecutive days for two hours.We used a binocular phase combination task to measure the subjects’sensory eye balances.We initially measured their baseline of sensory eye balance,patched their dominant eye,and then conducted post-patching measurements at 0,3,6,12,24 and 48 minutes after patching.Results:We performed a 2-way ANOVA(Before vs.after patching×Day);we found that although the effect of monocular deprivation on the deprived eye was significant,F(1,9)=17.32,P=0.002,the effect of Day was not.Conclusions:Hence we found no accumulation of the patching effect across five days in healthy adults.This suggests that the degree of remnant neural plasticity in adult primary visual cortex may be too limited to be exploited therapeutically.展开更多
AIM: Many studies have demonstrated N-methyl-D- aspartate receptor-1-subunit (NMDAR1) is associated with amblyopia. The effectiveness of levodopa in improving the visual function of the children with amblyopia has als...AIM: Many studies have demonstrated N-methyl-D- aspartate receptor-1-subunit (NMDAR1) is associated with amblyopia. The effectiveness of levodopa in improving the visual function of the children with amblyopia has also been proved. But the mechanism is undefined. Our study was to explore the possible mechanism.METHODS: Sixty 14-day-old healthy SD rats were randomly divided into 4 groups, including normal group, monocular deprivation group, levodopa group and normal saline group, 15 rats each. We sutured all the rats' unilateral eyelids except normal group to establish the monocular deprivation animal model and raise them in normal sunlight till 45-day-old. NMDAR1 was detected in the visual cortex with immunohistochemistry methods, Western Blot and Real time PCR. LD and NS groups were gavaged with levodopa(40mg/kg) and normal saline for 28 days respectively. NMDAR1 was also detected with the methods above.RESULTS: NMDAR1 in the visual cortex of MD group was less than that of normal group. NMDAR1 in the visual cortex of LD group was more than that of NS group.CONCLUSION: NMDAR1 is associated with the plasticity of visual development. Levodopa may influence the expression of NMDAR1 and improve visual function, and its target may lie in the visual cortex.展开更多
Purpose: To assess the effects of monocular lid closure during critical period on cortical activity.Method: Pattern visual evoked potentials (PVEP) of the normal and the monocular deprivation (MD) cats were dynamicall...Purpose: To assess the effects of monocular lid closure during critical period on cortical activity.Method: Pattern visual evoked potentials (PVEP) of the normal and the monocular deprivation (MD) cats were dynamically measured and the number of gam-maaminobutyric acid immunopositive (GABA-IP) neurones of the area 17 of the visual cortex and the lateral geniculate nucleus (LGN) was quantitatively compared by using immunohistochemical method (ABC).Result: The amplitude of the N1-P1 attenuated in deprived eyes (DE) , NE/DE at postnatal week (PNW) 7-8 (P【0. 05), NE/DE at PNW 15-16 (P【0. 01); while P1 latency delayed, NE/DE at PNW 7-8 (P】0. 05), NE/DE at PNW 15 - 16(P【0. 05). The numbers of GABA-IP neurones in layer A1 of the ipsilateral LGN and in layer A of the contralateral LGN, compared to those in the corresponding normal laminae, were not significant at PNW 7 - 8 and PNW 11 - 12 (P 】0. 05), while in the same cats a reduction in the number of GABA-IP neurcnes was found in layer IV of area 17 at PNW展开更多
Monocular 3D object detection is challenging due to the lack of accurate depth information.Some methods estimate the pixel-wise depth maps from off-the-shelf depth estimators and then use them as an additional input t...Monocular 3D object detection is challenging due to the lack of accurate depth information.Some methods estimate the pixel-wise depth maps from off-the-shelf depth estimators and then use them as an additional input to augment the RGB images.Depth-based methods attempt to convert estimated depth maps to pseudo-LiDAR and then use LiDAR-based object detectors or focus on the perspective of image and depth fusion learning.However,they demonstrate limited performance and efficiency as a result of depth inaccuracy and complex fusion mode with convolutions.Different from these approaches,our proposed depth-guided vision transformer with a normalizing flows(NF-DVT)network uses normalizing flows to build priors in depth maps to achieve more accurate depth information.Then we develop a novel Swin-Transformer-based backbone with a fusion module to process RGB image patches and depth map patches with two separate branches and fuse them using cross-attention to exchange information with each other.Furthermore,with the help of pixel-wise relative depth values in depth maps,we develop new relative position embeddings in the cross-attention mechanism to capture more accurate sequence ordering of input tokens.Our method is the first Swin-Transformer-based backbone architecture for monocular 3D object detection.The experimental results on the KITTI and the challenging Waymo Open datasets show the effectiveness of our proposed method and superior performance over previous counterparts.展开更多
BACKGROUND Sleep deprivation is a prevalent issue that impacts cognitive function.Although numerous neuroimaging studies have explored the neural correlates of sleep loss,inconsistencies persist in the reported result...BACKGROUND Sleep deprivation is a prevalent issue that impacts cognitive function.Although numerous neuroimaging studies have explored the neural correlates of sleep loss,inconsistencies persist in the reported results,necessitating an investigation into the consistent brain functional changes resulting from sleep loss.AIM To establish the consistency of brain functional alterations associated with sleep deprivation through systematic searches of neuroimaging databases.Two metaanalytic methods,signed differential mapping(SDM)and activation likelihood estimation(ALE),were employed to analyze functional magnetic resonance imaging(fMRI)data.METHODS A systematic search performed according to PRISMA guidelines was conducted across multiple databases through July 29,2023.Studies that met specific inclusion criteria,focused on healthy subjects with acute sleep deprivation and reported whole-brain functional data in English were considered.A total of 21 studies were selected for SDM and ALE meta-analyses.RESULTS Twenty-one studies,including 23 experiments and 498 subjects,were included.Compared to pre-sleep deprivation,post-sleep deprivation brain function was associated with increased gray matter in the right corpus callosum and decreased activity in the left medial frontal gyrus and left inferior parietal lobule.SDM revealed increased brain functional activity in the left striatum and right central posterior gyrus and decreased activity in the right cerebellar gyrus,left middle frontal gyrus,corpus callosum,and right cuneus.CONCLUSION This meta-analysis consistently identified brain regions affected by sleep deprivation,notably the left medial frontal gyrus and corpus callosum,shedding light on the neuropathology of sleep deprivation and offering insights into its neurological impact.展开更多
Objective:To explore the changes of lateral geniculate body and visual cortex in monocular strabismus and form deprived amblyopic rat,and visual development plastic stage and visual plasticity in adult rats.Methods:A ...Objective:To explore the changes of lateral geniculate body and visual cortex in monocular strabismus and form deprived amblyopic rat,and visual development plastic stage and visual plasticity in adult rats.Methods:A total of 60 SD rats ages 13 d were randomly divided into A,B,C three groups with 20 in each group,group A was set as the normal control group without any processing,group B was strabismus amblyopic group,using the unilateral extraocular rectus resection to establish the strabismus amblyopia model,group C was monocular form deprivation amblyopia group using unilateral eyelid edge resection+lid suture.At visual developmental early phase(P2S),meta phase(P3S),late phase(P45)and adult phase(P120),the lateral geniculate body and visual cortex area 17 of five rats in each group were exacted for C-fos Immunocytochemistry.Neuron morphological changes in lateral geniculate body and visual cortex was observed,the positive neurons differences of C-fos expression induced by light stimulation was measured in each group,and the condition of radiation development of P120 amblyopic adult rats was observed.Results:In groups B and C,C-fos positive cells were significantly lower than the control group at P25(P<0.05),there was no statistical difference of C-fos protein positive cells between group B and group A(P>0.05),C-fos protein positive cells level of group B was significantly lower than that of group A(P<0.05).The binoculus C-fos protein positive cells level of groups B and C were significantly higher than that of control group at P35,P4S and P120 with statistically significant differences(P<0.05).Conclusions:The increasing of C-fos expression in geniculate body and visual cortex neurons of adult amblyopia suggests the visual cortex neurons exist a certain degree of visual plasticity.展开更多
Sleep benefits the restoration of energy metabolism and thereby suppo rts neuronal plasticity and cognitive behaviors.Sirt6 is a NAD+-dependent protein deacetylase that has been recognized as an essential regulator of...Sleep benefits the restoration of energy metabolism and thereby suppo rts neuronal plasticity and cognitive behaviors.Sirt6 is a NAD+-dependent protein deacetylase that has been recognized as an essential regulator of energy metabolism because it modulates various transcriptional regulators and metabolic enzymes.The aim of this study was to investigate the influence of Sirt6 on cerebral function after chronic sleep deprivation(CSD).We assigned C57BL/6J mice to control or two CSD groups and subjected them to AAV2/9-CMV-EGFP or AAV2/9-CMV-Sirt6-EGFP infection in the prelimbic cortex(PrL).We then assessed cerebral functional connectivity(FC) using resting-state functional MRI,neuron/astrocyte metabolism using a metabolic kinetics analysis;dendritic spine densities using sparse-labeling;and miniature excitato ry postsynaptic currents(mEPSCs) and action potential(AP) firing rates using whole-cell patchclamp recordings.In addition,we evaluated cognition via a comprehensive set of behavioral tests.Compared with controls,Sirt6 was significantly decreased(P<0.05) in the PrL after CSD,accompanied by cognitive deficits and decreased FC between the PrL and accumbens nucleus,piriform cortex,motor co rtex,somatosensory co rtex,olfactory tubercle,insular cortex,and cerebellum.Sirt6 ove rexpression reve rsed CSD-induced cognitive impairment and reduced FC.Our analysis of metabolic kinetics using [1-13C] glucose and [2-13C] acetate showed that CSD reduced neuronal Glu4and GABA2synthesis,which could be fully restored via forced Sirt6 expression.Furthermore,Sirt6 ove rexpression reversed CSD-induced decreases in AP firing rates as well as the frequency and amplitude of mEPSCs in PrL pyramidal neurons.These data indicate that Sirt6 can improve cognitive impairment after CSD by regulating the PrL-associated FC network,neuronal glucose metabolism,and glutamatergic neurotransmission.Thus,Sirt6 activation may have potential as a novel strategy for treating sleep disorder-related diseases.展开更多
Studies on ischemia/reperfusion(I/R)injury suggest that exogenous neural stem cells(NSCs)are ideal candidates for stem cell therapy reperfusion injury.However,NSCs are difficult to obtain owing to ethical limitations....Studies on ischemia/reperfusion(I/R)injury suggest that exogenous neural stem cells(NSCs)are ideal candidates for stem cell therapy reperfusion injury.However,NSCs are difficult to obtain owing to ethical limitations.In addition,the survival,differentiation,and proliferation rates of transplanted exogenous NSCs are low,which limit their clinical application.Our previous study showed that neuregulin1β(NRG1β)alleviated cerebral I/R injury in rats.In this study,we aimed to induce human umbilical cord mesenchymal stem cells into NSCs and investigate the improvement effect and mechanism of NSCs pretreated with 10 nM NRG1βon PC12 cells injured by oxygen-glucose deprivation/reoxygenation(OGD/R).Our results found that 5 and 10 nM NRG1βpromoted the generation and proliferation of NSCs.Co-culture of NSCs and PC12 cells under condition of OGD/R showed that pretreatment of NSCs with NRG1βimproved the level of reactive oxygen species,malondialdehyde,glutathione,superoxide dismutase,nicotinamide adenine dinucleotide phosphate,and nuclear factor erythroid 2-related factor 2(Nrf2)and mitochondrial damage in injured PC12 cells;these indexes are related to ferroptosis.Research has reported that p53 and solute carrier family 7 member 11(SLC7A11)play vital roles in ferroptosis caused by cerebral I/R injury.Our data show that the expression of p53 was increased and the level of glutathione peroxidase 4(GPX4)was decreased after RNA interference-mediated knockdown of SLC7A11 in PC12 cells,but this change was alleviated after co-culturing NSCs with damaged PC12 cells.These findings suggest that NSCs pretreated with NRG1βexhibited neuroprotective effects on PC12 cells subjected to OGD/R through influencing the level of ferroptosis regulated by p53/SLC7A11/GPX4 pathway.展开更多
In the developing world,vulnerable communities often lack access to regular income sources to cope with unforeseen events.Recent advancements in financial technology have enabled microcredit to be delivered via digita...In the developing world,vulnerable communities often lack access to regular income sources to cope with unforeseen events.Recent advancements in financial technology have enabled microcredit to be delivered via digital platforms.Although digital credit may quicken remote access to consumer credit without the need for collateral,little is known about its contribution to the welfare of underserved communities.This study examines the effects of local digital lending development on deprivation and explores the implications of these effects on rural inhabitants.The results show a negative association between local digital lending development and food deprivation on one hand and health deprivation on the other.The evidence suggests that local digital lending development can reduce the probability of food and health deprivation.Furthermore,the evidence reveals that inhabitants of rural communities benefit more from digital lending development.This study recommends the decentralization of financial inclusion policies as a pathway to promote digital lending at the local level.展开更多
Objective: To evaluate the effect of salidroside on oxygen and glucose deprivation(OGD)-treated NT2 cells and its underlying mechanisms of action.Methods: Retinoic acid was used to induce the differentiation of NT2 ce...Objective: To evaluate the effect of salidroside on oxygen and glucose deprivation(OGD)-treated NT2 cells and its underlying mechanisms of action.Methods: Retinoic acid was used to induce the differentiation of NT2 cells into neurons. The effects of salidroside on survival, apoptosis, inflammatory response, and oxidative stress of neurons undergoing OGD were evaluated. Using precursor cells as controls, the effect of salidroside on the differentiation progression of OGDtreated cells was evaluated. In addition, the effect of erastin, a ferroptosis inducer, on NT2 cells was examined to investigate the underlying mechanisms of neuroprotective action of salidroside.Results: Salidroside alleviated the effects of OGD on neuronal survival, apoptosis, inflammation, and oxidative stress, and promoted NT2 cell differentiation. Moreover, salidroside prevented ferroptosis of OGD-treated cells, which was abolished following erastin treatment, indicating that ferroptosis mediated the regulatory pathway of salidroside.Conclusions: Salidroside attenuates OGD-induced neuronal injury by inhibiting ferroptosis and promotes neuronal differentiation.展开更多
The high bandwidth and low latency of 6G network technology enable the successful application of monocular 3D object detection on vehicle platforms.Monocular 3D-object-detection-based Pseudo-LiDAR is a low-cost,lowpow...The high bandwidth and low latency of 6G network technology enable the successful application of monocular 3D object detection on vehicle platforms.Monocular 3D-object-detection-based Pseudo-LiDAR is a low-cost,lowpower solution compared to LiDAR solutions in the field of autonomous driving.However,this technique has some problems,i.e.,(1)the poor quality of generated Pseudo-LiDAR point clouds resulting from the nonlinear error distribution of monocular depth estimation and(2)the weak representation capability of point cloud features due to the neglected global geometric structure features of point clouds existing in LiDAR-based 3D detection networks.Therefore,we proposed a Pseudo-LiDAR confidence sampling strategy and a hierarchical geometric feature extraction module for monocular 3D object detection.We first designed a point cloud confidence sampling strategy based on a 3D Gaussian distribution to assign small confidence to the points with great error in depth estimation and filter them out according to the confidence.Then,we present a hierarchical geometric feature extraction module by aggregating the local neighborhood features and a dual transformer to capture the global geometric features in the point cloud.Finally,our detection framework is based on Point-Voxel-RCNN(PV-RCNN)with high-quality Pseudo-LiDAR and enriched geometric features as input.From the experimental results,our method achieves satisfactory results in monocular 3D object detection.展开更多
Monocular depth estimation is the basic task in computer vision.Its accuracy has tremendous improvement in the decade with the development of deep learning.However,the blurry boundary in the depth map is a serious pro...Monocular depth estimation is the basic task in computer vision.Its accuracy has tremendous improvement in the decade with the development of deep learning.However,the blurry boundary in the depth map is a serious problem.Researchers find that the blurry boundary is mainly caused by two factors.First,the low-level features,containing boundary and structure information,may be lost in deep networks during the convolution process.Second,themodel ignores the errors introduced by the boundary area due to the few portions of the boundary area in the whole area,during the backpropagation.Focusing on the factors mentioned above.Two countermeasures are proposed to mitigate the boundary blur problem.Firstly,we design a scene understanding module and scale transformmodule to build a lightweight fuse feature pyramid,which can deal with low-level feature loss effectively.Secondly,we propose a boundary-aware depth loss function to pay attention to the effects of the boundary’s depth value.Extensive experiments show that our method can predict the depth maps with clearer boundaries,and the performance of the depth accuracy based on NYU-Depth V2,SUN RGB-D,and iBims-1 are competitive.展开更多
AIM:To construct an in vitro model of oxygen-glucose deprivation/reperfusion(OGD/R)induced injury to the optic nerve and to study the oxidative damage mechanism of ischemia-reperfusion(I/R)injury in 661W cells and the...AIM:To construct an in vitro model of oxygen-glucose deprivation/reperfusion(OGD/R)induced injury to the optic nerve and to study the oxidative damage mechanism of ischemia-reperfusion(I/R)injury in 661W cells and the protective effect of ginsenoside Rg1.METHODS:The 661W cells were treated with different concentrations of Na2S2O4 to establish OGD/R model in vitro.Apoptosis,intracellular reactive oxygen species(ROS)levels and superoxide dismutase(SOD)levels were measured at different time points during the reperfusion injury process.The injury model was pretreated with graded concentrations of ginsenoside Rg1.Real-time polymerase chain reaction(PCR)was used to measure the expression levels of cytochrome C(cyt C)/B-cell lymphoma-2(Bcl2)/Bcl2 associated protein X(Bax),heme oxygenase-1(HO-1),caspase9,nuclear factor erythroid 2-related factor 2(nrf2),kelch-like ECH-associated protein 1(keap1)and other genes.Western blot was used to detect the expression of nrf2,phosphorylated nrf2(pnrf2)and keap1 protein levels.RESULTS:Compared to the untreated group,the cell activity of 661W cells treated with Na2S2O4 for 6 and 8h decreased(P<0.01).Additionally,the ROS content increased and SOD levels decreased significantly(P<0.01).In contrast,treatment with ginsenoside Rg1 reversed the cell viability and SOD levels in comparison to the Na_(2)S_(2)O_(4)treated group(P<0.01).Moreover,Rg1 reduced the levels of caspase3,caspase9,and cyt C,while increasing the Bcl2/Bax level.These differences were all statistically significant(P<0.05).Western blot analysis showed no significant difference in the protein expression levels of keap1 and nrf2 with Rg1 treatment,however,Rg1 significantly increased the ratio of pnrf2/nrf2 protein expression compared to the Na_(2)S_(2)O_(4)treated group(P<0.001).CONCLUSION:The OGD/R process is induced in 661W cells using Na_(2)S_(2)O_(4).Rg1 inhibits OGD/R-induced oxidative damage and alleviates the extent of apoptosis in 661W cells through the keap1/nrf2 pathway.These results suggest a potential protective effect of Rg1 against retinal I/R injury.展开更多
Monocular 6D pose estimation is a functional task in the field of com-puter vision and robotics.In recent years,2D-3D correspondence-based methods have achieved improved performance in multiview and depth data-based s...Monocular 6D pose estimation is a functional task in the field of com-puter vision and robotics.In recent years,2D-3D correspondence-based methods have achieved improved performance in multiview and depth data-based scenes.However,for monocular 6D pose estimation,these methods are affected by the prediction results of the 2D-3D correspondences and the robustness of the per-spective-n-point(PnP)algorithm.There is still a difference in the distance from the expected estimation effect.To obtain a more effective feature representation result,edge enhancement is proposed to increase the shape information of the object by analyzing the influence of inaccurate 2D-3D matching on 6D pose regression and comparing the effectiveness of the intermediate representation.Furthermore,although the transformation matrix is composed of rotation and translation matrices from 3D model points to 2D pixel points,the two variables are essentially different and the same network cannot be used for both variables in the regression process.Therefore,to improve the effectiveness of the PnP algo-rithm,this paper designs a dual-branch PnP network to predict rotation and trans-lation information.Finally,the proposed method is verified on the public LM,LM-O and YCB-Video datasets.The ADD(S)values of the proposed method are 94.2 and 62.84 on the LM and LM-O datasets,respectively.The AUC of ADD(-S)value on YCB-Video is 81.1.These experimental results show that the performance of the proposed method is superior to that of similar methods.展开更多
Obejective:To explore the establishment of an oxygen glucose deprivation/reperfusion model of senescent SH-SY5Y cells.Methods:SH-SY5Y cells were randomly divided into control(D-galactose 0 mmol/L group),D-galactose(25...Obejective:To explore the establishment of an oxygen glucose deprivation/reperfusion model of senescent SH-SY5Y cells.Methods:SH-SY5Y cells were randomly divided into control(D-galactose 0 mmol/L group),D-galactose(25 mmol/L,50 mmol/L,100 mmol/L,200 mmol/L,400 mmol/L)groups,and treated with corresponding concentrations of D-galactose for 48 h.The changes of cell morphology,β-galactosidase,the cell morphology,β-galactosidase activity by microscopic observation,cell proliferation rate by EdU kit and cell survival rate by CCK-8 assay were used to determine the decaying concentration of D-galactose and to establish the senescence model.The senescent SH-SY5Y cells were randomly divided into control group(oxygen glucose deprivation without treatment group),oxygen glucose deprivation treatment(0.5 h,1 h,1.5 h,2 h)group,followed by re-glucose reoxygenation for 24 h,and CCK-8 assay for the survival rate of senescent SH-SY5Y cells.Results:There were no significant changes in cell morphology and β-gal activity in the 25 mmol/L and 50 mmol/L groups compared with the control group(P>0.05),cytosolic hypertrophy was seen in the cells of the 100 mmol/L group,chromatin fixation in the cells of the 200 mmol/L group,and massive vacuolization in the cells of the 400 mmol/L group;the positive rate ofβ-galactosidase staining in the cells of the(100-400 mmol/L)group was significantly higher compared with the control group(P<0.05),with little difference between the 100 mmol/L and 200 mmol/L groups(P>0.05);the cell proliferation ability of the(100-400 mmol/L)group was significantly decreased in a concentration-dependent manner(P<0.05);the cell survival rate was decreased in a concentration-dependent manner(P<0.05),with IC_(50) between 100 mmol/L and 200 mmol/L.The survival of senescent SH-SY5Y cells showed a time-dependent decrease in oxygen-glucose deprivation(P<0.05),with an IC_(50) close to 1 h.Conclusion:D-gal concentration of 100 mmoL/L and 48 h of cell action could establish a survival rate of about 50%of senescent SH-SY5Y cells,and oxygen glucose deprivation of senescent SH-SY5Y cells for 1 h and reperfusion for 24 h could establish an oxygen glucose deprivation/reperfusion model of senescent SH-SY5Y cells with a survival rate close to 50%.展开更多
文摘Background:Short term monocular deprivation allows for the modulation of ocular dominance,such that the previously deprived eye contribution will increase,while that of non-deprived eye will decrease.This study examines the effects of short monocular occlusion on the Pulfrich phenomenon,an illusory perception of a horizontally moving object moving in an elliptical orbit in depth.In addition,we will explore whether the modulation of the Pulfrich effect is produced in the magnocellular pathway or the parvocellular pathway,by comparing two protocols,each designed to activate one pathway at a time.Methods:The stimulus used throughout the experiment is made up of elements defining a cylinder rotating in depth,allowing to measure interocular delay.The task consists of reporting the direction of rotation of the stimulus presented.There are two different stimuli:the P stimulus is composed of small elements oscillating slowly,which stimulates the parvocellular pathway,and the M stimulus which is composed of large elements oscillating rapidly which stimulates the magnocellular pathway.One experimental session consists of pre-patch testing,one hour of patching,and a post-patch testing.Each participant performs four sessions,both stimuli for each eye.Results:The point of subjective equivalence(PSE)is extracted from psychometric functions obtained during pre-testing and post-testing.Following deprivation of the left eye the PSE shifts negatively,whereas deprivation of the right eye shifts the PSE positively on the psychometric function.This indicated that monocular deprivation slows the perceptual processes of the previously patched eye.The amplitude of this effect is larger for the M protocol than it is for the P protocol.Conclusions:Contrary to expectations,results showed that effects of monocular deprivation are not exclusively mediated by contrast gain mechanisms,as suggested by Zhou and colleagues(2014).The amplitude of the differences observed for the M protocole suggest that the plasticity induced by short term deprivation is equally subjected to dynamic components.
文摘Background:Short-term monocular deprivation has been recently shown to temporarily increase the sensitivity of the patched eye.Many studies have patched subjects for an arbitrary period of 2.5 hours,but for no principled reason.Our goal is to show a relationship,if any,between the length of patching duration and the strength of its effect.Methods:We tested nine subjects with three different patching durations:1-,2-,3-hour.Four of the nine subjects were patched for 5-hour.Monocular deprivation was achieved by the use of a translucent eyepatch.A session included two rounds of baseline testing of interocular eye balance,patching,and post-patching tests.Each post-patching test occurred at 0,3,6,12,24,48,60 and 96 minutes after patching to track the patching effect over time.Every subject performed two sessions per condition.Results:One-hour patching produced a small shift in ocular dominance.A larger shift occurred from 2-hour patching,but 3-hour patching produced a comparable effect to the one measured after 2-hour patching.Conclusions:These results show a saturation of the patching effect beyond 2-hour patching.Hence,we believe that 2-hour patching duration is the optimal duration for eye dominance changes induced by monocular deprivation.
文摘Background:We investigate whether changes in visual plasticity induced by monocular deprivation can be maintained across multiple days.It has been known that monocular deprivation strengthens the deprived eye in adults with normal vision for a short period of time(30-60 minutes).This has been shown through a variety of visual tasks such as binocular combination and rivalry.Methods:Ten subjects were recruited and patched for five consecutive days for two hours.We used a binocular phase combination task to measure the subjects’sensory eye balances.We initially measured their baseline of sensory eye balance,patched their dominant eye,and then conducted post-patching measurements at 0,3,6,12,24 and 48 minutes after patching.Results:We performed a 2-way ANOVA(Before vs.after patching×Day);we found that although the effect of monocular deprivation on the deprived eye was significant,F(1,9)=17.32,P=0.002,the effect of Day was not.Conclusions:Hence we found no accumulation of the patching effect across five days in healthy adults.This suggests that the degree of remnant neural plasticity in adult primary visual cortex may be too limited to be exploited therapeutically.
文摘AIM: Many studies have demonstrated N-methyl-D- aspartate receptor-1-subunit (NMDAR1) is associated with amblyopia. The effectiveness of levodopa in improving the visual function of the children with amblyopia has also been proved. But the mechanism is undefined. Our study was to explore the possible mechanism.METHODS: Sixty 14-day-old healthy SD rats were randomly divided into 4 groups, including normal group, monocular deprivation group, levodopa group and normal saline group, 15 rats each. We sutured all the rats' unilateral eyelids except normal group to establish the monocular deprivation animal model and raise them in normal sunlight till 45-day-old. NMDAR1 was detected in the visual cortex with immunohistochemistry methods, Western Blot and Real time PCR. LD and NS groups were gavaged with levodopa(40mg/kg) and normal saline for 28 days respectively. NMDAR1 was also detected with the methods above.RESULTS: NMDAR1 in the visual cortex of MD group was less than that of normal group. NMDAR1 in the visual cortex of LD group was more than that of NS group.CONCLUSION: NMDAR1 is associated with the plasticity of visual development. Levodopa may influence the expression of NMDAR1 and improve visual function, and its target may lie in the visual cortex.
文摘Purpose: To assess the effects of monocular lid closure during critical period on cortical activity.Method: Pattern visual evoked potentials (PVEP) of the normal and the monocular deprivation (MD) cats were dynamically measured and the number of gam-maaminobutyric acid immunopositive (GABA-IP) neurones of the area 17 of the visual cortex and the lateral geniculate nucleus (LGN) was quantitatively compared by using immunohistochemical method (ABC).Result: The amplitude of the N1-P1 attenuated in deprived eyes (DE) , NE/DE at postnatal week (PNW) 7-8 (P【0. 05), NE/DE at PNW 15-16 (P【0. 01); while P1 latency delayed, NE/DE at PNW 7-8 (P】0. 05), NE/DE at PNW 15 - 16(P【0. 05). The numbers of GABA-IP neurones in layer A1 of the ipsilateral LGN and in layer A of the contralateral LGN, compared to those in the corresponding normal laminae, were not significant at PNW 7 - 8 and PNW 11 - 12 (P 】0. 05), while in the same cats a reduction in the number of GABA-IP neurcnes was found in layer IV of area 17 at PNW
基金supported in part by the Major Project for New Generation of AI (2018AAA0100400)the National Natural Science Foundation of China (61836014,U21B2042,62072457,62006231)the InnoHK Program。
文摘Monocular 3D object detection is challenging due to the lack of accurate depth information.Some methods estimate the pixel-wise depth maps from off-the-shelf depth estimators and then use them as an additional input to augment the RGB images.Depth-based methods attempt to convert estimated depth maps to pseudo-LiDAR and then use LiDAR-based object detectors or focus on the perspective of image and depth fusion learning.However,they demonstrate limited performance and efficiency as a result of depth inaccuracy and complex fusion mode with convolutions.Different from these approaches,our proposed depth-guided vision transformer with a normalizing flows(NF-DVT)network uses normalizing flows to build priors in depth maps to achieve more accurate depth information.Then we develop a novel Swin-Transformer-based backbone with a fusion module to process RGB image patches and depth map patches with two separate branches and fuse them using cross-attention to exchange information with each other.Furthermore,with the help of pixel-wise relative depth values in depth maps,we develop new relative position embeddings in the cross-attention mechanism to capture more accurate sequence ordering of input tokens.Our method is the first Swin-Transformer-based backbone architecture for monocular 3D object detection.The experimental results on the KITTI and the challenging Waymo Open datasets show the effectiveness of our proposed method and superior performance over previous counterparts.
文摘BACKGROUND Sleep deprivation is a prevalent issue that impacts cognitive function.Although numerous neuroimaging studies have explored the neural correlates of sleep loss,inconsistencies persist in the reported results,necessitating an investigation into the consistent brain functional changes resulting from sleep loss.AIM To establish the consistency of brain functional alterations associated with sleep deprivation through systematic searches of neuroimaging databases.Two metaanalytic methods,signed differential mapping(SDM)and activation likelihood estimation(ALE),were employed to analyze functional magnetic resonance imaging(fMRI)data.METHODS A systematic search performed according to PRISMA guidelines was conducted across multiple databases through July 29,2023.Studies that met specific inclusion criteria,focused on healthy subjects with acute sleep deprivation and reported whole-brain functional data in English were considered.A total of 21 studies were selected for SDM and ALE meta-analyses.RESULTS Twenty-one studies,including 23 experiments and 498 subjects,were included.Compared to pre-sleep deprivation,post-sleep deprivation brain function was associated with increased gray matter in the right corpus callosum and decreased activity in the left medial frontal gyrus and left inferior parietal lobule.SDM revealed increased brain functional activity in the left striatum and right central posterior gyrus and decreased activity in the right cerebellar gyrus,left middle frontal gyrus,corpus callosum,and right cuneus.CONCLUSION This meta-analysis consistently identified brain regions affected by sleep deprivation,notably the left medial frontal gyrus and corpus callosum,shedding light on the neuropathology of sleep deprivation and offering insights into its neurological impact.
文摘Objective:To explore the changes of lateral geniculate body and visual cortex in monocular strabismus and form deprived amblyopic rat,and visual development plastic stage and visual plasticity in adult rats.Methods:A total of 60 SD rats ages 13 d were randomly divided into A,B,C three groups with 20 in each group,group A was set as the normal control group without any processing,group B was strabismus amblyopic group,using the unilateral extraocular rectus resection to establish the strabismus amblyopia model,group C was monocular form deprivation amblyopia group using unilateral eyelid edge resection+lid suture.At visual developmental early phase(P2S),meta phase(P3S),late phase(P45)and adult phase(P120),the lateral geniculate body and visual cortex area 17 of five rats in each group were exacted for C-fos Immunocytochemistry.Neuron morphological changes in lateral geniculate body and visual cortex was observed,the positive neurons differences of C-fos expression induced by light stimulation was measured in each group,and the condition of radiation development of P120 amblyopic adult rats was observed.Results:In groups B and C,C-fos positive cells were significantly lower than the control group at P25(P<0.05),there was no statistical difference of C-fos protein positive cells between group B and group A(P>0.05),C-fos protein positive cells level of group B was significantly lower than that of group A(P<0.05).The binoculus C-fos protein positive cells level of groups B and C were significantly higher than that of control group at P35,P4S and P120 with statistically significant differences(P<0.05).Conclusions:The increasing of C-fos expression in geniculate body and visual cortex neurons of adult amblyopia suggests the visual cortex neurons exist a certain degree of visual plasticity.
基金National Natural Science Foundation of China,Nos.81771160 (to ZZ),81671060 (to CC),31970973 (to JW),21921004 (to FX)Translational Medicine and In terdisciplinary Research Joint Fund of Zhongnan Hospital of Wuhan University,No.ZNJC201934 (to ZZ)。
文摘Sleep benefits the restoration of energy metabolism and thereby suppo rts neuronal plasticity and cognitive behaviors.Sirt6 is a NAD+-dependent protein deacetylase that has been recognized as an essential regulator of energy metabolism because it modulates various transcriptional regulators and metabolic enzymes.The aim of this study was to investigate the influence of Sirt6 on cerebral function after chronic sleep deprivation(CSD).We assigned C57BL/6J mice to control or two CSD groups and subjected them to AAV2/9-CMV-EGFP or AAV2/9-CMV-Sirt6-EGFP infection in the prelimbic cortex(PrL).We then assessed cerebral functional connectivity(FC) using resting-state functional MRI,neuron/astrocyte metabolism using a metabolic kinetics analysis;dendritic spine densities using sparse-labeling;and miniature excitato ry postsynaptic currents(mEPSCs) and action potential(AP) firing rates using whole-cell patchclamp recordings.In addition,we evaluated cognition via a comprehensive set of behavioral tests.Compared with controls,Sirt6 was significantly decreased(P<0.05) in the PrL after CSD,accompanied by cognitive deficits and decreased FC between the PrL and accumbens nucleus,piriform cortex,motor co rtex,somatosensory co rtex,olfactory tubercle,insular cortex,and cerebellum.Sirt6 ove rexpression reve rsed CSD-induced cognitive impairment and reduced FC.Our analysis of metabolic kinetics using [1-13C] glucose and [2-13C] acetate showed that CSD reduced neuronal Glu4and GABA2synthesis,which could be fully restored via forced Sirt6 expression.Furthermore,Sirt6 ove rexpression reversed CSD-induced decreases in AP firing rates as well as the frequency and amplitude of mEPSCs in PrL pyramidal neurons.These data indicate that Sirt6 can improve cognitive impairment after CSD by regulating the PrL-associated FC network,neuronal glucose metabolism,and glutamatergic neurotransmission.Thus,Sirt6 activation may have potential as a novel strategy for treating sleep disorder-related diseases.
基金supported by the National Natural Science Foundation of China,No.81973501the Natural Science Foundation of Shandong Province,No.ZR2019MH009(both to YLG).
文摘Studies on ischemia/reperfusion(I/R)injury suggest that exogenous neural stem cells(NSCs)are ideal candidates for stem cell therapy reperfusion injury.However,NSCs are difficult to obtain owing to ethical limitations.In addition,the survival,differentiation,and proliferation rates of transplanted exogenous NSCs are low,which limit their clinical application.Our previous study showed that neuregulin1β(NRG1β)alleviated cerebral I/R injury in rats.In this study,we aimed to induce human umbilical cord mesenchymal stem cells into NSCs and investigate the improvement effect and mechanism of NSCs pretreated with 10 nM NRG1βon PC12 cells injured by oxygen-glucose deprivation/reoxygenation(OGD/R).Our results found that 5 and 10 nM NRG1βpromoted the generation and proliferation of NSCs.Co-culture of NSCs and PC12 cells under condition of OGD/R showed that pretreatment of NSCs with NRG1βimproved the level of reactive oxygen species,malondialdehyde,glutathione,superoxide dismutase,nicotinamide adenine dinucleotide phosphate,and nuclear factor erythroid 2-related factor 2(Nrf2)and mitochondrial damage in injured PC12 cells;these indexes are related to ferroptosis.Research has reported that p53 and solute carrier family 7 member 11(SLC7A11)play vital roles in ferroptosis caused by cerebral I/R injury.Our data show that the expression of p53 was increased and the level of glutathione peroxidase 4(GPX4)was decreased after RNA interference-mediated knockdown of SLC7A11 in PC12 cells,but this change was alleviated after co-culturing NSCs with damaged PC12 cells.These findings suggest that NSCs pretreated with NRG1βexhibited neuroprotective effects on PC12 cells subjected to OGD/R through influencing the level of ferroptosis regulated by p53/SLC7A11/GPX4 pathway.
文摘In the developing world,vulnerable communities often lack access to regular income sources to cope with unforeseen events.Recent advancements in financial technology have enabled microcredit to be delivered via digital platforms.Although digital credit may quicken remote access to consumer credit without the need for collateral,little is known about its contribution to the welfare of underserved communities.This study examines the effects of local digital lending development on deprivation and explores the implications of these effects on rural inhabitants.The results show a negative association between local digital lending development and food deprivation on one hand and health deprivation on the other.The evidence suggests that local digital lending development can reduce the probability of food and health deprivation.Furthermore,the evidence reveals that inhabitants of rural communities benefit more from digital lending development.This study recommends the decentralization of financial inclusion policies as a pathway to promote digital lending at the local level.
基金supported by the Zhejiang Traditional Chinese Medicine Science and Technology Plan Project(2021ZB027,2023ZL267)Zhejiang Medical and Health Platform Project of China(2019KY002,2019RC092).
文摘Objective: To evaluate the effect of salidroside on oxygen and glucose deprivation(OGD)-treated NT2 cells and its underlying mechanisms of action.Methods: Retinoic acid was used to induce the differentiation of NT2 cells into neurons. The effects of salidroside on survival, apoptosis, inflammatory response, and oxidative stress of neurons undergoing OGD were evaluated. Using precursor cells as controls, the effect of salidroside on the differentiation progression of OGDtreated cells was evaluated. In addition, the effect of erastin, a ferroptosis inducer, on NT2 cells was examined to investigate the underlying mechanisms of neuroprotective action of salidroside.Results: Salidroside alleviated the effects of OGD on neuronal survival, apoptosis, inflammation, and oxidative stress, and promoted NT2 cell differentiation. Moreover, salidroside prevented ferroptosis of OGD-treated cells, which was abolished following erastin treatment, indicating that ferroptosis mediated the regulatory pathway of salidroside.Conclusions: Salidroside attenuates OGD-induced neuronal injury by inhibiting ferroptosis and promotes neuronal differentiation.
基金supported by the National Key Research and Development Program of China(2020YFB1807500)the National Natural Science Foundation of China(62072360,62001357,62172438,61901367)+4 种基金the key research and development plan of Shaanxi province(2021ZDLGY02-09,2023-GHZD-44,2023-ZDLGY-54)the Natural Science Foundation of Guangdong Province of China(2022A1515010988)Key Project on Artificial Intelligence of Xi'an Science and Technology Plan(2022JH-RGZN-0003,2022JH-RGZN-0103,2022JH-CLCJ-0053)Xi'an Science and Technology Plan(20RGZN0005)the Proof-ofconcept fund from Hangzhou Research Institute of Xidian University(GNYZ2023QC0201).
文摘The high bandwidth and low latency of 6G network technology enable the successful application of monocular 3D object detection on vehicle platforms.Monocular 3D-object-detection-based Pseudo-LiDAR is a low-cost,lowpower solution compared to LiDAR solutions in the field of autonomous driving.However,this technique has some problems,i.e.,(1)the poor quality of generated Pseudo-LiDAR point clouds resulting from the nonlinear error distribution of monocular depth estimation and(2)the weak representation capability of point cloud features due to the neglected global geometric structure features of point clouds existing in LiDAR-based 3D detection networks.Therefore,we proposed a Pseudo-LiDAR confidence sampling strategy and a hierarchical geometric feature extraction module for monocular 3D object detection.We first designed a point cloud confidence sampling strategy based on a 3D Gaussian distribution to assign small confidence to the points with great error in depth estimation and filter them out according to the confidence.Then,we present a hierarchical geometric feature extraction module by aggregating the local neighborhood features and a dual transformer to capture the global geometric features in the point cloud.Finally,our detection framework is based on Point-Voxel-RCNN(PV-RCNN)with high-quality Pseudo-LiDAR and enriched geometric features as input.From the experimental results,our method achieves satisfactory results in monocular 3D object detection.
基金supported in part by School Research Projects of Wuyi University (No.5041700175).
文摘Monocular depth estimation is the basic task in computer vision.Its accuracy has tremendous improvement in the decade with the development of deep learning.However,the blurry boundary in the depth map is a serious problem.Researchers find that the blurry boundary is mainly caused by two factors.First,the low-level features,containing boundary and structure information,may be lost in deep networks during the convolution process.Second,themodel ignores the errors introduced by the boundary area due to the few portions of the boundary area in the whole area,during the backpropagation.Focusing on the factors mentioned above.Two countermeasures are proposed to mitigate the boundary blur problem.Firstly,we design a scene understanding module and scale transformmodule to build a lightweight fuse feature pyramid,which can deal with low-level feature loss effectively.Secondly,we propose a boundary-aware depth loss function to pay attention to the effects of the boundary’s depth value.Extensive experiments show that our method can predict the depth maps with clearer boundaries,and the performance of the depth accuracy based on NYU-Depth V2,SUN RGB-D,and iBims-1 are competitive.
基金Supported by Natural Science Foundation of Guangdong Province(No.2021A1515010513)。
文摘AIM:To construct an in vitro model of oxygen-glucose deprivation/reperfusion(OGD/R)induced injury to the optic nerve and to study the oxidative damage mechanism of ischemia-reperfusion(I/R)injury in 661W cells and the protective effect of ginsenoside Rg1.METHODS:The 661W cells were treated with different concentrations of Na2S2O4 to establish OGD/R model in vitro.Apoptosis,intracellular reactive oxygen species(ROS)levels and superoxide dismutase(SOD)levels were measured at different time points during the reperfusion injury process.The injury model was pretreated with graded concentrations of ginsenoside Rg1.Real-time polymerase chain reaction(PCR)was used to measure the expression levels of cytochrome C(cyt C)/B-cell lymphoma-2(Bcl2)/Bcl2 associated protein X(Bax),heme oxygenase-1(HO-1),caspase9,nuclear factor erythroid 2-related factor 2(nrf2),kelch-like ECH-associated protein 1(keap1)and other genes.Western blot was used to detect the expression of nrf2,phosphorylated nrf2(pnrf2)and keap1 protein levels.RESULTS:Compared to the untreated group,the cell activity of 661W cells treated with Na2S2O4 for 6 and 8h decreased(P<0.01).Additionally,the ROS content increased and SOD levels decreased significantly(P<0.01).In contrast,treatment with ginsenoside Rg1 reversed the cell viability and SOD levels in comparison to the Na_(2)S_(2)O_(4)treated group(P<0.01).Moreover,Rg1 reduced the levels of caspase3,caspase9,and cyt C,while increasing the Bcl2/Bax level.These differences were all statistically significant(P<0.05).Western blot analysis showed no significant difference in the protein expression levels of keap1 and nrf2 with Rg1 treatment,however,Rg1 significantly increased the ratio of pnrf2/nrf2 protein expression compared to the Na_(2)S_(2)O_(4)treated group(P<0.001).CONCLUSION:The OGD/R process is induced in 661W cells using Na_(2)S_(2)O_(4).Rg1 inhibits OGD/R-induced oxidative damage and alleviates the extent of apoptosis in 661W cells through the keap1/nrf2 pathway.These results suggest a potential protective effect of Rg1 against retinal I/R injury.
基金This work was supported by the National Natural Science Foundation of China(No.61871196 and 62001176)the Natural Science Foundation of Fujian Province of China(No.2019J01082 and 2020J01085)the Promotion Program for Young and Middle-aged Teachers in Science and Technology Research of Huaqiao University(ZQN-YX601).
文摘Monocular 6D pose estimation is a functional task in the field of com-puter vision and robotics.In recent years,2D-3D correspondence-based methods have achieved improved performance in multiview and depth data-based scenes.However,for monocular 6D pose estimation,these methods are affected by the prediction results of the 2D-3D correspondences and the robustness of the per-spective-n-point(PnP)algorithm.There is still a difference in the distance from the expected estimation effect.To obtain a more effective feature representation result,edge enhancement is proposed to increase the shape information of the object by analyzing the influence of inaccurate 2D-3D matching on 6D pose regression and comparing the effectiveness of the intermediate representation.Furthermore,although the transformation matrix is composed of rotation and translation matrices from 3D model points to 2D pixel points,the two variables are essentially different and the same network cannot be used for both variables in the regression process.Therefore,to improve the effectiveness of the PnP algo-rithm,this paper designs a dual-branch PnP network to predict rotation and trans-lation information.Finally,the proposed method is verified on the public LM,LM-O and YCB-Video datasets.The ADD(S)values of the proposed method are 94.2 and 62.84 on the LM and LM-O datasets,respectively.The AUC of ADD(-S)value on YCB-Video is 81.1.These experimental results show that the performance of the proposed method is superior to that of similar methods.
基金This is supported by the Youth Science Foundation of Guangxi Medical University(GXMUYSF202127)。
文摘Obejective:To explore the establishment of an oxygen glucose deprivation/reperfusion model of senescent SH-SY5Y cells.Methods:SH-SY5Y cells were randomly divided into control(D-galactose 0 mmol/L group),D-galactose(25 mmol/L,50 mmol/L,100 mmol/L,200 mmol/L,400 mmol/L)groups,and treated with corresponding concentrations of D-galactose for 48 h.The changes of cell morphology,β-galactosidase,the cell morphology,β-galactosidase activity by microscopic observation,cell proliferation rate by EdU kit and cell survival rate by CCK-8 assay were used to determine the decaying concentration of D-galactose and to establish the senescence model.The senescent SH-SY5Y cells were randomly divided into control group(oxygen glucose deprivation without treatment group),oxygen glucose deprivation treatment(0.5 h,1 h,1.5 h,2 h)group,followed by re-glucose reoxygenation for 24 h,and CCK-8 assay for the survival rate of senescent SH-SY5Y cells.Results:There were no significant changes in cell morphology and β-gal activity in the 25 mmol/L and 50 mmol/L groups compared with the control group(P>0.05),cytosolic hypertrophy was seen in the cells of the 100 mmol/L group,chromatin fixation in the cells of the 200 mmol/L group,and massive vacuolization in the cells of the 400 mmol/L group;the positive rate ofβ-galactosidase staining in the cells of the(100-400 mmol/L)group was significantly higher compared with the control group(P<0.05),with little difference between the 100 mmol/L and 200 mmol/L groups(P>0.05);the cell proliferation ability of the(100-400 mmol/L)group was significantly decreased in a concentration-dependent manner(P<0.05);the cell survival rate was decreased in a concentration-dependent manner(P<0.05),with IC_(50) between 100 mmol/L and 200 mmol/L.The survival of senescent SH-SY5Y cells showed a time-dependent decrease in oxygen-glucose deprivation(P<0.05),with an IC_(50) close to 1 h.Conclusion:D-gal concentration of 100 mmoL/L and 48 h of cell action could establish a survival rate of about 50%of senescent SH-SY5Y cells,and oxygen glucose deprivation of senescent SH-SY5Y cells for 1 h and reperfusion for 24 h could establish an oxygen glucose deprivation/reperfusion model of senescent SH-SY5Y cells with a survival rate close to 50%.
