The herb Butea monosperma constitutes several human health beneficial components, which are mostly studied for their anticancer effects. In this study, the activity of n-butanol fractions of B. monosperma floral extra...The herb Butea monosperma constitutes several human health beneficial components, which are mostly studied for their anticancer effects. In this study, the activity of n-butanol fractions of B. monosperma floral extract was examined on inhibiting aberrant crypt foci(ACF) formation in azoxymethane induced Wistar albino rats. The n-butanol extracts(150 mg/kg) decreased the ACF formation(per rat) by 92% and78% in short- and long-term in vivo treatments, respectively. All the compounds in the n-butanol extract were isolated and purified using column and reverse-phase high pressure liquid chromatography(HPLC).Their structures were characterized using UV–visible spectroscopy, nuclear magnetic resonance(NMR)and electrospray–ionisation mass spectrometry(ESI–MS) to determine important flavonoids, namely isocoreopsin, butrin and isobutrin. These compounds were studied for their free radical scavenging and anticancer activities. The compound isocoreopsin showed significantly greater efficacy in cell death on human colon and liver cancer cell lines(50 μg/m L in HT-29 and 100 μg/m L in Hep G2) than butrin(100 μg/m L in HT-29 and 500 μg/m L in Hep G2) and isobutrin(80 μg/m L in HT-29 and 150 μg/m L in Hep G2). These results suggest that isocoreopsin, butrin and isobutrin are the important key compounds for the chemoprevention of colon cancer and isocoreopsin can be considered as a promising novel drug.展开更多
Atlantic forest natural soil flora was tested on two leguminous pioneer species Acacia gummifera and Retama monosperma to be used in rehabilitation programs of the coastal sand dunes forest of the Essaouira region.The...Atlantic forest natural soil flora was tested on two leguminous pioneer species Acacia gummifera and Retama monosperma to be used in rehabilitation programs of the coastal sand dunes forest of the Essaouira region.The rhizospheric soil of two endemic plant species:R.nonosperma and Juniperus phoenicea was sampled and split into two categories,one from native rhizospheric soil,the other of the sterilized rhizospheric soil.Investigation was focused on mycorrhizal formations,but other forms of beneficial symbiosis such as rhizobia and viable soil microflora were also studied.Growth and nutrition variables assessed included lengths of roots and shoots,number of branches,ratio of root to shoot dry mass and water,nitrogen and phosphorus levels.Results showed important mycorrhizal associations in roots of both plants,presence of nodules and abundance of viable soil microflora.J.phoenicea had a 100%frequency of mycorrhizal formation and an intensity of 80%compared to a frequency of 80%and intensity of 54%for R.monosperma.Nodules had the same density of CFU regardless of the origin.Abundance of viable microflora in rhisospheric soil of R.monosperma,J.phoenicea and the control differed significantly.Among the studied variables for A.gummifera and R.monosperma,nitrogen and phosphorus uptakes significantly wit the use of the nonsterilized rhizospheric soil.Both species doubled their phosphorus uptake when colonized by mycorrhizal species,R.monosperma doubled its nitrogen uptake and A.gummifera increased it by seven times compared with the control.No significant difference was noted for the other variables.展开更多
The drug L-DOPA has been widely used against Parkinson's disease and is extracted from plants.Due to the increasing demand of this drug,new plant sources need to be discovered in addition to the existing sources.T...The drug L-DOPA has been widely used against Parkinson's disease and is extracted from plants.Due to the increasing demand of this drug,new plant sources need to be discovered in addition to the existing sources.The paper embodies results on Mucuna monosperma,which can be a promising candidate for L-DOPA.The seed powder of this plant contains 5.48%of(dry weight)the drug and when the seeds were soaked in distilled water,content was increased to 6.58%.Different elicitors when added,enhanced the drug level in seed up to 11.8%.The possible rationale behind this increase was confirmed by increase in tyrosinase activity in the seeds.Presence of L-DOPA was confirmed using various analytical techniques as HPLC,HPTLC and NMR.The work demonstrates a potential candidate plant as a source for L-DOPA when a novel method was adopted as described here.展开更多
Prescribed fire produced a landscape with two types of severely burned patches: charred shrub patches and charred patches with tree trunks at the center. Soil nematodes were more abundant in burned and unburned junipe...Prescribed fire produced a landscape with two types of severely burned patches: charred shrub patches and charred patches with tree trunks at the center. Soil nematodes were more abundant in burned and unburned juniper (Juniperus monosperma) tree patches than in yucca-shrub patches. There were no differences in nematode abundance between burned and unburned patches during the late spring and summer samples. Nematode abundance was significantly (p < 0.05) higher in unburned patches than in burned patches in the early spring samples, reflecting large differences in soil moisture between unburned and burned patches. There were no differences in soil nematode abundance between burned and unburned patches at oneyear post-burn and three-year post-burn sites. When all samples were pooled, taxonomic diversity, ecological indices, and abundance of trophic groups (bacteria-feeders, fungi-feeders, and omnivore-predators) were higher in unburned than burned patches. These results suggest that the long-term (up to three years post-burn) effects of fire on soil nematodes are indirect, i.e., by loss of tree canopies, litter accumulation, and shrub foliage, which affects soil temperatures and water redistribution.展开更多
基金financially supported by University Grant Commission(UGC)New Delhi,India(Grant No:F.41-1289/2012(SR)/dt.26.07.2012)
文摘The herb Butea monosperma constitutes several human health beneficial components, which are mostly studied for their anticancer effects. In this study, the activity of n-butanol fractions of B. monosperma floral extract was examined on inhibiting aberrant crypt foci(ACF) formation in azoxymethane induced Wistar albino rats. The n-butanol extracts(150 mg/kg) decreased the ACF formation(per rat) by 92% and78% in short- and long-term in vivo treatments, respectively. All the compounds in the n-butanol extract were isolated and purified using column and reverse-phase high pressure liquid chromatography(HPLC).Their structures were characterized using UV–visible spectroscopy, nuclear magnetic resonance(NMR)and electrospray–ionisation mass spectrometry(ESI–MS) to determine important flavonoids, namely isocoreopsin, butrin and isobutrin. These compounds were studied for their free radical scavenging and anticancer activities. The compound isocoreopsin showed significantly greater efficacy in cell death on human colon and liver cancer cell lines(50 μg/m L in HT-29 and 100 μg/m L in Hep G2) than butrin(100 μg/m L in HT-29 and 500 μg/m L in Hep G2) and isobutrin(80 μg/m L in HT-29 and 150 μg/m L in Hep G2). These results suggest that isocoreopsin, butrin and isobutrin are the important key compounds for the chemoprevention of colon cancer and isocoreopsin can be considered as a promising novel drug.
基金This work was supported by the Cadi Ayyad University and"Laboratoire Ecologie et Environnement(L2E)"an associated unit to the "Centre National de la Recherche Scientifique et Technologique(CNRST)"URAC32.
文摘Atlantic forest natural soil flora was tested on two leguminous pioneer species Acacia gummifera and Retama monosperma to be used in rehabilitation programs of the coastal sand dunes forest of the Essaouira region.The rhizospheric soil of two endemic plant species:R.nonosperma and Juniperus phoenicea was sampled and split into two categories,one from native rhizospheric soil,the other of the sterilized rhizospheric soil.Investigation was focused on mycorrhizal formations,but other forms of beneficial symbiosis such as rhizobia and viable soil microflora were also studied.Growth and nutrition variables assessed included lengths of roots and shoots,number of branches,ratio of root to shoot dry mass and water,nitrogen and phosphorus levels.Results showed important mycorrhizal associations in roots of both plants,presence of nodules and abundance of viable soil microflora.J.phoenicea had a 100%frequency of mycorrhizal formation and an intensity of 80%compared to a frequency of 80%and intensity of 54%for R.monosperma.Nodules had the same density of CFU regardless of the origin.Abundance of viable microflora in rhisospheric soil of R.monosperma,J.phoenicea and the control differed significantly.Among the studied variables for A.gummifera and R.monosperma,nitrogen and phosphorus uptakes significantly wit the use of the nonsterilized rhizospheric soil.Both species doubled their phosphorus uptake when colonized by mycorrhizal species,R.monosperma doubled its nitrogen uptake and A.gummifera increased it by seven times compared with the control.No significant difference was noted for the other variables.
文摘The drug L-DOPA has been widely used against Parkinson's disease and is extracted from plants.Due to the increasing demand of this drug,new plant sources need to be discovered in addition to the existing sources.The paper embodies results on Mucuna monosperma,which can be a promising candidate for L-DOPA.The seed powder of this plant contains 5.48%of(dry weight)the drug and when the seeds were soaked in distilled water,content was increased to 6.58%.Different elicitors when added,enhanced the drug level in seed up to 11.8%.The possible rationale behind this increase was confirmed by increase in tyrosinase activity in the seeds.Presence of L-DOPA was confirmed using various analytical techniques as HPLC,HPTLC and NMR.The work demonstrates a potential candidate plant as a source for L-DOPA when a novel method was adopted as described here.
文摘Prescribed fire produced a landscape with two types of severely burned patches: charred shrub patches and charred patches with tree trunks at the center. Soil nematodes were more abundant in burned and unburned juniper (Juniperus monosperma) tree patches than in yucca-shrub patches. There were no differences in nematode abundance between burned and unburned patches during the late spring and summer samples. Nematode abundance was significantly (p < 0.05) higher in unburned patches than in burned patches in the early spring samples, reflecting large differences in soil moisture between unburned and burned patches. There were no differences in soil nematode abundance between burned and unburned patches at oneyear post-burn and three-year post-burn sites. When all samples were pooled, taxonomic diversity, ecological indices, and abundance of trophic groups (bacteria-feeders, fungi-feeders, and omnivore-predators) were higher in unburned than burned patches. These results suggest that the long-term (up to three years post-burn) effects of fire on soil nematodes are indirect, i.e., by loss of tree canopies, litter accumulation, and shrub foliage, which affects soil temperatures and water redistribution.
文摘北美樱桃核桧于1987 年引入呼和浩特市树木园,经10a 的引种驯化与3a的无性繁殖试验,证明该树种比当地其他柏类树种更耐旱、耐寒,无病虫害,生长旺盛。经分析北美樱桃核桧生长规律吻合于指数增长方程。10 年生平均树高2 .5 m ,最高达3 .2 m ,针叶呈淡蓝灰绿色,冠形优美,为温带半干旱地区庭院、街道、公园等地绿化的优良新树种。本文着重报道了其生态习性、引种驯化与栽培管理及扦插繁殖技术。
