Objective:To investigate the anti-diabetic effect of the root extract of Annona muricata(AME)in streptozotocin-nicotinamide-induced type 2 diabetic(T2DM)mice.Methods:After 4 weeks of high-fat diet,ICR mice were given ...Objective:To investigate the anti-diabetic effect of the root extract of Annona muricata(AME)in streptozotocin-nicotinamide-induced type 2 diabetic(T2DM)mice.Methods:After 4 weeks of high-fat diet,ICR mice were given 1 g/kg nicotine and 120 mg/kg streptozotocin(STZ)orally to construct a T2DM model.The T2DM mice were randomly divided into five groups:model group,200 mg/kg metformin group and 50,100,200 mg/kg AME groups.Drugs were oral administered continuously for 4 weeks.Fasting blood glucose and body weight were measured weekly.Oral glucose tolerance test(OGTT)and detection of serum glycated hemoglobin(HbA1c)level were performed one week before the end of the experiment.At the end of drug administration,serum total cholesterol(TG),triglycerides(TC),low-density lipoprotein levels(LDL-C)and insulin levels were tested by lipid detection kits;homeostasis model assessment-estimated insulin resistance(HOMA-IR)and HOMA-βindexes were calculated.Liver and kidney tissues were weighed to calculate organ indices and pathological tests were performed.Western blot was performed in the liver to detect adenosine monophosphate-activated protein kinase(AMPK),acetyl coenzyme A carboxylase(ACC),glucose-6-phosphate carboxylase(G6Pase),and phosphoenolpyruvate carboxykinase(PCK1)protein expression.Results:with 200 mg/kg AME significantly reduced fasting blood glucose,HbA1c,TG and LDL-C levels,protected liver and kindey in diabetic mice,decreased the area under the OGTT curve,inhibited ACC and G6Pase protein expressions,and activated AMPK protein expression.Conclusion:AME showed good therapeutic activity against T2DM,and the mechanism may be related to the activation of AMPK and inhibition of ACC and G6Pase proteins.展开更多
Objective:To explore the anti-diabetic effects and its underlying mechanism of Annona muricata Linn fruit ethanol extract(AME).Methods:Streptozotocin-induced type 2 diabetic(T2DM)mouse model was constructed.Those diab...Objective:To explore the anti-diabetic effects and its underlying mechanism of Annona muricata Linn fruit ethanol extract(AME).Methods:Streptozotocin-induced type 2 diabetic(T2DM)mouse model was constructed.Those diabetic mice were randomly grouped and given 50 mg/kg acarbose or AME(200 mg/kg,100 mg/kg or 50 mg/kg)for four weeks.The body weight,postprandial blood glucose and glycosylated hemoglobin levels were measured during the administration.After the administration,a glucose tolerance test was performed,and the levels of triglycerides,cholesterol and low-density lipoproteins in mice were detected by biochemical test kits.The inhibitory activity of AME onα-glucosidase in vivo and in vitro was determined by enzyme inhibition tests.Results:AME significantly reduced weight gain,postprandial blood glucose,glycosylated hemoglobin and low-density lipoprotein levels in T2DM mice;enhanced glucose tolerance and pancreaticβ-cell function of T2DM mice;inhibitedα-glucosidase activity in mouse intestine in an noncompetitive manner.Conclusion:AME may noncompetitive inhibitα-glucosidase activity and reduce postprandial glucose intake to achieve a therapeutic and regulatory effect on type 2 diabetes.展开更多
Annona muricata(A.muricata)is a tropical plant species belonging to family Annonaceae and known for its many medicinal uses.This review focuses on the research history of its traditional uses,phytochemicals,pharmacolo...Annona muricata(A.muricata)is a tropical plant species belonging to family Annonaceae and known for its many medicinal uses.This review focuses on the research history of its traditional uses,phytochemicals,pharmacological activities,toxicological aspects of the extracts and isolated compounds,as well as the in vitro propagation studies with the objective of stimulating further studies on this plant for human consumption and treatment.A.muricata extracts have been identified in tropical regions to traditionally treat diverse conditions ranging from fever to diabetes and cancer.More than 200 chemical compounds have been identified and isolated from this plant,the most important being alkaloids,phenols and acetogenins.Using in vitro studies,its extracts and phytochemicals have been characterized as antioxidant,anti-microbial,anti-inflammatory,insecticidal,larvicidal,and cytotoxic to cancer cells.In vivo studies have revealed anxiolytic,antistress,anti-inflammatory,immunomodulatory,antimalarial,antidepressant,gastro protective,wound healing,hepato-protective,hypoglycemic,anticancer and anti-tumoral activities.In silico studies have also been reported.In addition,clinical studies support the hypoglycemic as well as some anticancer activities.Mechanisms of action of some pharmacological activities have been elucidated.However,some phytochemical compounds isolated from A.muricata have shown a neurotoxic effect in vitro and in vivo,and therefore,these crude extracts and isolated compounds need to be further investigated to define the magnitude of the effects,optimal dosage,and mechanisms of action,long-term safety,and potential side effects.Additionally,more clinical studies are necessary to support the therapeutic potential of this plant.Some studies were also found to have successfully regenerated the plant in vitro,but with limited success.The reported toxicity notwithstanding,A.muricata extracts seem to be some of the safest and promising therapeutic agents of the 21st century and beyond that need to be studied further for better medicinal formulations and diseases management.展开更多
<i><span style="font-family:"">Annona</span></i><span style="font-family:""> <i>muricata</i> L. and <i>Annona</i> <i>squamosa&l...<i><span style="font-family:"">Annona</span></i><span style="font-family:""> <i>muricata</i> L. and <i>Annona</i> <i>squamosa</i> L. are tropical species whose</span><span style="font-family:""> fleshy fruit is edible. They offer real possibilities for socio-economic use, particularly in the fields of medicine, nutrition, ecosystem conservation and the poverty alleviation. This study was set up to evaluate different methods of micropropagation from juvenile material for the regeneration of these species. Thus, MS medium supplemented with [BAP 2 mg·L<sup>-1</sup>] <i>i.e.</i> M2 produced 2.87 newly <span>formed shoots from the cotyledonary nodes of <i>A.</i> <i>muricata</i>. For the terminal apices of <i>A.</i> <i>squamosa</i>, it was MMS medium supplemented with [BAP 2</span> mg·L<sup>-1</sup>] <i>i.e.</i> MM2 that was most conducive to new shoot formation (3.12). The addition of 0.1 and 0.2 mg·L<sup>-1</sup> of NAA in the M2 medium, made it possi<span>ble to have the best elongations and average number of nodes for the new shoots from cotyledonary nodes of <i>A.</i> <i>muricata</i> (9.11 cm for 5.62 nodes). With <i>A.</i> <i>squamosa</i>, MM7 medium [MMS + BAP 1 mg·L<sup>-1</sup></span></span><span style="font-family:""> </span><span style="font-family:"">+ KIN 1 mg·L<sup>-1</sup>]</span><span style="font-family:""> resulted in an average length of 9.05 cm with 5.62 nodes on average for the apical shoots. A 3-day rhizogenic induction period in the dark with [IBA 50 mg·L<sup>-1</sup>] and 2 g·L<sup>-1</sup> of activated charcoal gave a rooting rate of 66.67% for shoots originating from the cotyledonary nodes of <i>A.</i> <i>squamosa</i>;while with vitroplants from cotyledonary nodes of <i>A.</i> <i>muricata</i>, a better rooting rate (83.33%) was obtained following a 5-day rhizogenic induction. After 30 days of acclimatization, the survival rate reached 83.33% for plants from the tips of <i>A.</i> <i>muricata</i>, whereas for <i>A.</i> <i>squamosa,</i> it was plants grown from cotyledonary nodes that had the same survival rate.展开更多
Muricatenol 1. a new acetogenin. has been isolated from the seeds of .Annona, muricata L.. Compound 1 is a C37, acetogenin without any THF rings. with four hydroxyls and one double bond in the long aliphatic chain. Th...Muricatenol 1. a new acetogenin. has been isolated from the seeds of .Annona, muricata L.. Compound 1 is a C37, acetogenin without any THF rings. with four hydroxyls and one double bond in the long aliphatic chain. The hydroxyls of I are located at C-4. C-10. C-18 and C-19. respectively. The vicinal diol at C-18/C-19 is threo-configuration. and the double bond at 14/C-15 is cis-configuration.展开更多
Objective: To identify the phytochemical compounds from Annona muricata(A. muricata) and to determine their in vitro anti-proliferative activities against breast cancer cells, MCF7 and MDA-MB-231. Methods: A. muricata...Objective: To identify the phytochemical compounds from Annona muricata(A. muricata) and to determine their in vitro anti-proliferative activities against breast cancer cells, MCF7 and MDA-MB-231. Methods: A. muricata leaves were successively extracted by soxhlet method using n-hexane, ethyl acetate and methanol, and decocted with water. Each extract was analysed by gas chromatography mass spectrometry(GCMS) and characterized with Wiley and NIST library searches. Anti-proliferative activity of each extract was evaluated on MCF7 and MDA-MB-231 breast cancer cells using MTT assay. Results: The GCMS analysis of different solvent extracts of A. muricata leaves showed presence of different chemical groups of compounds such as steroids, terpenoids, phenolic compounds, sugars, sugars alcohol and others including vitamin E. Ethyl acetate leaves extract exhibited the lowest IC_(50) value on the MDA-MB-231 breast cancer cell and n-hexane leaves extract showed the the lowest IC_(50) value on the MCF-7 breast cancer cell. Conclusion: Steroids and phenolic compounds were the main phytocompound groups identified from all A. muricata leaves extracts. The antiproliferative activity of n-hexane and ethyl acetate extract towards breast cancer MCF7 and MDA-MB-231 respectively might be due to the presence of biologically active compounds in the extracts, hence, providing some scientific evidences of the effectiveness of its traditional usages.展开更多
Objective: To analyze anticancer activity of an ethyl acetate extract of endophytic fungi isolated from soursop leaf(Annona muricata L.). Methods: Anticancer activity of fungal extracts was determined by observing its...Objective: To analyze anticancer activity of an ethyl acetate extract of endophytic fungi isolated from soursop leaf(Annona muricata L.). Methods: Anticancer activity of fungal extracts was determined by observing its toxicity against MCF-7(Michigan Cancer Foundation-7) cells in vitro by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay method. At an extract concentration of 100 μg/m L, 4 isolates out of 12 showed high activity against the cancer cell growth. The four isolates were then selected for further IC50 determination, by measuring the inhibition of cancer cell proliferation at extract concentration of 25 μg/m L, 50 μg/m L, 100 μg/m L, 200 μg/m L and 400 μg/m L. Results: Results showed that isolate Sir-G5 had the highest anticancer activity with an IC50 of 19.20 μg/m L. The best isolates were screened again using a normal cell(Chang cells) to determine its toxicity against normal cells. Results indicated that the extracts do not affect the proliferation of normal cells. Molecular identification showed that the fungal isolate Sir-G5 has a close relationship with Phomopsis sp. Conclusions: The endophytic fungi isolated from soursop leaf has the potential to be used as a source of anticancer agents.展开更多
Introduction: Now many studies conducted on the drug substance from nature that can serve as an anticancer agent as a potential chemoprevention agent, such as Annona muricata Linn leaf escort chemotherapy, which was f...Introduction: Now many studies conducted on the drug substance from nature that can serve as an anticancer agent as a potential chemoprevention agent, such as Annona muricata Linn leaf escort chemotherapy, which was flaring. The cancer cell in humans was included the loss of p53 protein function due to mutations in the protein gene. Other causes are that the p53 proteins are not functioning due to an increase in protein misfolding event chaperones and degradation events ubiquitous as binding by viral protein. Method: Cytotoxicity assay performed on 24 well plate micro-cultures. HeLa cells are as 2 × 104 cells in 100 mL in RPMI media. Created control is RPMI and solvent DMSO 0.25%. Cytotoxic Test performed by the method of calculation tryphan blue dye exclusion. Being fasted for 24 hours in the culture medium, then the cells are grown in micro-plate with media plus samples with a non-lethal concentration (LC50) of partition and fractionation Annona muricata Linn leaf. Sampling is performed at 24 hours. Each of these wells is calculated the number of living cells and made the curve of cell number and incubation time. Result: The results showed that HeLa cells are being LC50 partition of leaves Annona muricata Linn in ethyl acetate his cell death rate was higher (2000 μg/ml have 131.89%;15.625 μg/ml have 11.37%) and in ethanol-distillate water his cell death rate was lower (2000 μg/ml have 35.80%;15.625 μg/ml have 3.97%). Another results showed that HeLa cells are being LC50 fractionation of leaves Annona muricata Linn in chloroform his cell death rate was higher (2000 μg/ml have 91.86%;15.625 μg/ml have 2.68%) and in ethyl acetate, his cell death rate was lower (2000 μg/ml have 23.79%;15.625展开更多
<i><span style="font-family:Verdana;">Annona</span></i><span style="font-family:""> <i><span style="font-family:Verdana;">muricata</span&...<i><span style="font-family:Verdana;">Annona</span></i><span style="font-family:""> <i><span style="font-family:Verdana;">muricata</span></i><span style="font-family:Verdana;"> L. (Soursop or Graviola) is a naturally occurring plant seen in Southern part of Africa, traditionally used in Benin to treat various diseases. The present study aimed to investigate phytochemical composition and antioxidant, anti-inflammatory activities of </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">muricata</span></i><span style="font-family:Verdana;"> leaves extracts. The secondary metabolites of ethanolic and hemi-ethanolic extracts were analysed by HPLC method. The DPPH and FRAP methods were used to evaluate the antioxidant activity. Inhibition of albumin denaturation method was used to evaluate anti-inflammatory activity of the tested extracts of which larval cytotoxicity was st</span><span><span style="font-family:Verdana;">udied. The major identified compounds were gallic acid, chlorogenic acid, cafeic acid, tannic acid, ferrulic acid, Rutin. Ascorbic acid exhibited the highest inhibition percentage (83.33% ± 0.50%) of DPPH radical with the lowest IC</span><sub><span style="font-family:Verdana;">50</span></sub><span style="font-family:Verdana;"> (45.1 ± 0.28 μg/ml). The inhibition of the ferric ion Fe</span><sup><span style="font-family:Verdana;">3+</span></sup><span style="font-family:Verdana;"> varied (</span><i><span style="font-family:Verdana;">p</span></i><span style="font-family:Verdana;"> = 0.0013) according to the extracts type. IC</span><sub><span style="font-family:Verdana;">50</span></sub><span style="font-family:Verdana;"> values of ferric ion inhibition range from 119.5 ± 3.10 to 250.8 ± 2.13 μg/ml respectively for </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">muricata</span></i><span style="font-family:Verdana;"> leaves ethanol and hemi-ethanolic extracts. The hemi-ethanolic extract exhibited the highest anti-inflammatory activity (96.66% </span></span><span style="font-family:Verdana;">± 1.17%). The presence of phenolic compound confers to </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">muricata</span></i><span style="font-family:Verdana;"> leaves, through the ethanolic and the hemi-ethanolic extracts, the antioxidant and anti-inflammatory activities.展开更多
Raoiella indica Hirst, 1924 (Prostigmata: Tenuipalpidae), is one of the leading pest mites in palm and banana trees, however, there are few control methods available for this pest species. Therefore, this work aimed t...Raoiella indica Hirst, 1924 (Prostigmata: Tenuipalpidae), is one of the leading pest mites in palm and banana trees, however, there are few control methods available for this pest species. Therefore, this work aimed to evaluate the acaricidal effect of soursop seed extract (Annona muricata L.) on R. indica adults. The experiment was conducted in a completely randomized design using soursop seed extract with 7 replicates and 12 individuals of R. indica per replicate. The experimental units consisted of discs of coconut palm leaves (4 cm in diameter), with cotton moistened at the bottom of the Petri dish (10.0 × 1.2 cm) and around the disc to maintain turgor and prevent mites from escaping. The application was performed using an airbrush, connected to a calibrated compressor with a constant pressure of 1.3 psi and 1 mL of solution per repeat plate. The acaricidal effect was evaluated 24, 48, and 72 hours after spraying. Mortality data were corrected and submitted to Probit analysis (p ≤ 0.05) using the statistical program R, with the LC50 and LC<sub>90</sub> calculated for the extract. At the maximum concentration (15%), the soursop seed extract showed mortality of 70% of individuals of R. indica, and the LC50 was 6.58%. It was concluded that the soursop seed extract showed acaricidal potential on R. indica in the laboratory.展开更多
基金National Natural Science Foundation of China(No.81460591)Hainan Medical University Training Fund(HY2018-09)。
文摘Objective:To investigate the anti-diabetic effect of the root extract of Annona muricata(AME)in streptozotocin-nicotinamide-induced type 2 diabetic(T2DM)mice.Methods:After 4 weeks of high-fat diet,ICR mice were given 1 g/kg nicotine and 120 mg/kg streptozotocin(STZ)orally to construct a T2DM model.The T2DM mice were randomly divided into five groups:model group,200 mg/kg metformin group and 50,100,200 mg/kg AME groups.Drugs were oral administered continuously for 4 weeks.Fasting blood glucose and body weight were measured weekly.Oral glucose tolerance test(OGTT)and detection of serum glycated hemoglobin(HbA1c)level were performed one week before the end of the experiment.At the end of drug administration,serum total cholesterol(TG),triglycerides(TC),low-density lipoprotein levels(LDL-C)and insulin levels were tested by lipid detection kits;homeostasis model assessment-estimated insulin resistance(HOMA-IR)and HOMA-βindexes were calculated.Liver and kidney tissues were weighed to calculate organ indices and pathological tests were performed.Western blot was performed in the liver to detect adenosine monophosphate-activated protein kinase(AMPK),acetyl coenzyme A carboxylase(ACC),glucose-6-phosphate carboxylase(G6Pase),and phosphoenolpyruvate carboxykinase(PCK1)protein expression.Results:with 200 mg/kg AME significantly reduced fasting blood glucose,HbA1c,TG and LDL-C levels,protected liver and kindey in diabetic mice,decreased the area under the OGTT curve,inhibited ACC and G6Pase protein expressions,and activated AMPK protein expression.Conclusion:AME showed good therapeutic activity against T2DM,and the mechanism may be related to the activation of AMPK and inhibition of ACC and G6Pase proteins.
基金supported by 2020 College Students Innovation and Entrepreneurship Training Program(X202011810069)the National Natural Science Foundation of China(81460591)。
文摘Objective:To explore the anti-diabetic effects and its underlying mechanism of Annona muricata Linn fruit ethanol extract(AME).Methods:Streptozotocin-induced type 2 diabetic(T2DM)mouse model was constructed.Those diabetic mice were randomly grouped and given 50 mg/kg acarbose or AME(200 mg/kg,100 mg/kg or 50 mg/kg)for four weeks.The body weight,postprandial blood glucose and glycosylated hemoglobin levels were measured during the administration.After the administration,a glucose tolerance test was performed,and the levels of triglycerides,cholesterol and low-density lipoproteins in mice were detected by biochemical test kits.The inhibitory activity of AME onα-glucosidase in vivo and in vitro was determined by enzyme inhibition tests.Results:AME significantly reduced weight gain,postprandial blood glucose,glycosylated hemoglobin and low-density lipoprotein levels in T2DM mice;enhanced glucose tolerance and pancreaticβ-cell function of T2DM mice;inhibitedα-glucosidase activity in mouse intestine in an noncompetitive manner.Conclusion:AME may noncompetitive inhibitα-glucosidase activity and reduce postprandial glucose intake to achieve a therapeutic and regulatory effect on type 2 diabetes.
文摘Annona muricata(A.muricata)is a tropical plant species belonging to family Annonaceae and known for its many medicinal uses.This review focuses on the research history of its traditional uses,phytochemicals,pharmacological activities,toxicological aspects of the extracts and isolated compounds,as well as the in vitro propagation studies with the objective of stimulating further studies on this plant for human consumption and treatment.A.muricata extracts have been identified in tropical regions to traditionally treat diverse conditions ranging from fever to diabetes and cancer.More than 200 chemical compounds have been identified and isolated from this plant,the most important being alkaloids,phenols and acetogenins.Using in vitro studies,its extracts and phytochemicals have been characterized as antioxidant,anti-microbial,anti-inflammatory,insecticidal,larvicidal,and cytotoxic to cancer cells.In vivo studies have revealed anxiolytic,antistress,anti-inflammatory,immunomodulatory,antimalarial,antidepressant,gastro protective,wound healing,hepato-protective,hypoglycemic,anticancer and anti-tumoral activities.In silico studies have also been reported.In addition,clinical studies support the hypoglycemic as well as some anticancer activities.Mechanisms of action of some pharmacological activities have been elucidated.However,some phytochemical compounds isolated from A.muricata have shown a neurotoxic effect in vitro and in vivo,and therefore,these crude extracts and isolated compounds need to be further investigated to define the magnitude of the effects,optimal dosage,and mechanisms of action,long-term safety,and potential side effects.Additionally,more clinical studies are necessary to support the therapeutic potential of this plant.Some studies were also found to have successfully regenerated the plant in vitro,but with limited success.The reported toxicity notwithstanding,A.muricata extracts seem to be some of the safest and promising therapeutic agents of the 21st century and beyond that need to be studied further for better medicinal formulations and diseases management.
文摘<i><span style="font-family:"">Annona</span></i><span style="font-family:""> <i>muricata</i> L. and <i>Annona</i> <i>squamosa</i> L. are tropical species whose</span><span style="font-family:""> fleshy fruit is edible. They offer real possibilities for socio-economic use, particularly in the fields of medicine, nutrition, ecosystem conservation and the poverty alleviation. This study was set up to evaluate different methods of micropropagation from juvenile material for the regeneration of these species. Thus, MS medium supplemented with [BAP 2 mg·L<sup>-1</sup>] <i>i.e.</i> M2 produced 2.87 newly <span>formed shoots from the cotyledonary nodes of <i>A.</i> <i>muricata</i>. For the terminal apices of <i>A.</i> <i>squamosa</i>, it was MMS medium supplemented with [BAP 2</span> mg·L<sup>-1</sup>] <i>i.e.</i> MM2 that was most conducive to new shoot formation (3.12). The addition of 0.1 and 0.2 mg·L<sup>-1</sup> of NAA in the M2 medium, made it possi<span>ble to have the best elongations and average number of nodes for the new shoots from cotyledonary nodes of <i>A.</i> <i>muricata</i> (9.11 cm for 5.62 nodes). With <i>A.</i> <i>squamosa</i>, MM7 medium [MMS + BAP 1 mg·L<sup>-1</sup></span></span><span style="font-family:""> </span><span style="font-family:"">+ KIN 1 mg·L<sup>-1</sup>]</span><span style="font-family:""> resulted in an average length of 9.05 cm with 5.62 nodes on average for the apical shoots. A 3-day rhizogenic induction period in the dark with [IBA 50 mg·L<sup>-1</sup>] and 2 g·L<sup>-1</sup> of activated charcoal gave a rooting rate of 66.67% for shoots originating from the cotyledonary nodes of <i>A.</i> <i>squamosa</i>;while with vitroplants from cotyledonary nodes of <i>A.</i> <i>muricata</i>, a better rooting rate (83.33%) was obtained following a 5-day rhizogenic induction. After 30 days of acclimatization, the survival rate reached 83.33% for plants from the tips of <i>A.</i> <i>muricata</i>, whereas for <i>A.</i> <i>squamosa,</i> it was plants grown from cotyledonary nodes that had the same survival rate.
文摘Muricatenol 1. a new acetogenin. has been isolated from the seeds of .Annona, muricata L.. Compound 1 is a C37, acetogenin without any THF rings. with four hydroxyls and one double bond in the long aliphatic chain. The hydroxyls of I are located at C-4. C-10. C-18 and C-19. respectively. The vicinal diol at C-18/C-19 is threo-configuration. and the double bond at 14/C-15 is cis-configuration.
基金funded by the Universiti Sains Malaysia Short Term Grant(304/PPSP/6313322)
文摘Objective: To identify the phytochemical compounds from Annona muricata(A. muricata) and to determine their in vitro anti-proliferative activities against breast cancer cells, MCF7 and MDA-MB-231. Methods: A. muricata leaves were successively extracted by soxhlet method using n-hexane, ethyl acetate and methanol, and decocted with water. Each extract was analysed by gas chromatography mass spectrometry(GCMS) and characterized with Wiley and NIST library searches. Anti-proliferative activity of each extract was evaluated on MCF7 and MDA-MB-231 breast cancer cells using MTT assay. Results: The GCMS analysis of different solvent extracts of A. muricata leaves showed presence of different chemical groups of compounds such as steroids, terpenoids, phenolic compounds, sugars, sugars alcohol and others including vitamin E. Ethyl acetate leaves extract exhibited the lowest IC_(50) value on the MDA-MB-231 breast cancer cell and n-hexane leaves extract showed the the lowest IC_(50) value on the MCF-7 breast cancer cell. Conclusion: Steroids and phenolic compounds were the main phytocompound groups identified from all A. muricata leaves extracts. The antiproliferative activity of n-hexane and ethyl acetate extract towards breast cancer MCF7 and MDA-MB-231 respectively might be due to the presence of biologically active compounds in the extracts, hence, providing some scientific evidences of the effectiveness of its traditional usages.
基金supported by The Directorate General of Higher Education,Ministry of Education and Culture of The Republic of Indonesia
文摘Objective: To analyze anticancer activity of an ethyl acetate extract of endophytic fungi isolated from soursop leaf(Annona muricata L.). Methods: Anticancer activity of fungal extracts was determined by observing its toxicity against MCF-7(Michigan Cancer Foundation-7) cells in vitro by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay method. At an extract concentration of 100 μg/m L, 4 isolates out of 12 showed high activity against the cancer cell growth. The four isolates were then selected for further IC50 determination, by measuring the inhibition of cancer cell proliferation at extract concentration of 25 μg/m L, 50 μg/m L, 100 μg/m L, 200 μg/m L and 400 μg/m L. Results: Results showed that isolate Sir-G5 had the highest anticancer activity with an IC50 of 19.20 μg/m L. The best isolates were screened again using a normal cell(Chang cells) to determine its toxicity against normal cells. Results indicated that the extracts do not affect the proliferation of normal cells. Molecular identification showed that the fungal isolate Sir-G5 has a close relationship with Phomopsis sp. Conclusions: The endophytic fungi isolated from soursop leaf has the potential to be used as a source of anticancer agents.
文摘Introduction: Now many studies conducted on the drug substance from nature that can serve as an anticancer agent as a potential chemoprevention agent, such as Annona muricata Linn leaf escort chemotherapy, which was flaring. The cancer cell in humans was included the loss of p53 protein function due to mutations in the protein gene. Other causes are that the p53 proteins are not functioning due to an increase in protein misfolding event chaperones and degradation events ubiquitous as binding by viral protein. Method: Cytotoxicity assay performed on 24 well plate micro-cultures. HeLa cells are as 2 × 104 cells in 100 mL in RPMI media. Created control is RPMI and solvent DMSO 0.25%. Cytotoxic Test performed by the method of calculation tryphan blue dye exclusion. Being fasted for 24 hours in the culture medium, then the cells are grown in micro-plate with media plus samples with a non-lethal concentration (LC50) of partition and fractionation Annona muricata Linn leaf. Sampling is performed at 24 hours. Each of these wells is calculated the number of living cells and made the curve of cell number and incubation time. Result: The results showed that HeLa cells are being LC50 partition of leaves Annona muricata Linn in ethyl acetate his cell death rate was higher (2000 μg/ml have 131.89%;15.625 μg/ml have 11.37%) and in ethanol-distillate water his cell death rate was lower (2000 μg/ml have 35.80%;15.625 μg/ml have 3.97%). Another results showed that HeLa cells are being LC50 fractionation of leaves Annona muricata Linn in chloroform his cell death rate was higher (2000 μg/ml have 91.86%;15.625 μg/ml have 2.68%) and in ethyl acetate, his cell death rate was lower (2000 μg/ml have 23.79%;15.625
文摘<i><span style="font-family:Verdana;">Annona</span></i><span style="font-family:""> <i><span style="font-family:Verdana;">muricata</span></i><span style="font-family:Verdana;"> L. (Soursop or Graviola) is a naturally occurring plant seen in Southern part of Africa, traditionally used in Benin to treat various diseases. The present study aimed to investigate phytochemical composition and antioxidant, anti-inflammatory activities of </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">muricata</span></i><span style="font-family:Verdana;"> leaves extracts. The secondary metabolites of ethanolic and hemi-ethanolic extracts were analysed by HPLC method. The DPPH and FRAP methods were used to evaluate the antioxidant activity. Inhibition of albumin denaturation method was used to evaluate anti-inflammatory activity of the tested extracts of which larval cytotoxicity was st</span><span><span style="font-family:Verdana;">udied. The major identified compounds were gallic acid, chlorogenic acid, cafeic acid, tannic acid, ferrulic acid, Rutin. Ascorbic acid exhibited the highest inhibition percentage (83.33% ± 0.50%) of DPPH radical with the lowest IC</span><sub><span style="font-family:Verdana;">50</span></sub><span style="font-family:Verdana;"> (45.1 ± 0.28 μg/ml). The inhibition of the ferric ion Fe</span><sup><span style="font-family:Verdana;">3+</span></sup><span style="font-family:Verdana;"> varied (</span><i><span style="font-family:Verdana;">p</span></i><span style="font-family:Verdana;"> = 0.0013) according to the extracts type. IC</span><sub><span style="font-family:Verdana;">50</span></sub><span style="font-family:Verdana;"> values of ferric ion inhibition range from 119.5 ± 3.10 to 250.8 ± 2.13 μg/ml respectively for </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">muricata</span></i><span style="font-family:Verdana;"> leaves ethanol and hemi-ethanolic extracts. The hemi-ethanolic extract exhibited the highest anti-inflammatory activity (96.66% </span></span><span style="font-family:Verdana;">± 1.17%). The presence of phenolic compound confers to </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">muricata</span></i><span style="font-family:Verdana;"> leaves, through the ethanolic and the hemi-ethanolic extracts, the antioxidant and anti-inflammatory activities.
文摘Raoiella indica Hirst, 1924 (Prostigmata: Tenuipalpidae), is one of the leading pest mites in palm and banana trees, however, there are few control methods available for this pest species. Therefore, this work aimed to evaluate the acaricidal effect of soursop seed extract (Annona muricata L.) on R. indica adults. The experiment was conducted in a completely randomized design using soursop seed extract with 7 replicates and 12 individuals of R. indica per replicate. The experimental units consisted of discs of coconut palm leaves (4 cm in diameter), with cotton moistened at the bottom of the Petri dish (10.0 × 1.2 cm) and around the disc to maintain turgor and prevent mites from escaping. The application was performed using an airbrush, connected to a calibrated compressor with a constant pressure of 1.3 psi and 1 mL of solution per repeat plate. The acaricidal effect was evaluated 24, 48, and 72 hours after spraying. Mortality data were corrected and submitted to Probit analysis (p ≤ 0.05) using the statistical program R, with the LC50 and LC<sub>90</sub> calculated for the extract. At the maximum concentration (15%), the soursop seed extract showed mortality of 70% of individuals of R. indica, and the LC50 was 6.58%. It was concluded that the soursop seed extract showed acaricidal potential on R. indica in the laboratory.
