Background:Primary biliary cholangitis(PBC)is a chronic biliary autoimmune liver disease characterized by intrahepatic cholestasis.Swertia mussotii Franch.(SMF)is a Tibetan medicine with hepatoprotective and anti-infl...Background:Primary biliary cholangitis(PBC)is a chronic biliary autoimmune liver disease characterized by intrahepatic cholestasis.Swertia mussotii Franch.(SMF)is a Tibetan medicine with hepatoprotective and anti-inflammatory activities.In this study,the therapeutic effect and potential mechanisms of SMF on PBC were investigated by bioinformatics analysis and in vitro experimental validation,with the aim of promoting the progress of SMF and PBC research.Methods:We first explored the therapeutic effects and key targets of SMF on PBC using a network pharmacology approach,further screened the core targets using the GSE79850 dataset,and finally validated the results using molecular docking techniques and in vitro experiments.Results:By bioinformatics analysis,we identified core targets of SMF for PBC treatment(STAT3,JAK2,TNF-α,and IL-1β)and important signaling pathways:JAK-STAT,TNF,and PI3K-AKT.The molecular docking results showed that the significant components of SMF had good binding properties to the core targets.In vitro experiments showed that SMF extracts improved the extent of epithelial-mesenchymal transition in human intrahepatic biliary epithelial cells and had a significant reversal effect on epithelial-mesenchymal transition process markers and potential targets in PBC.Conclusion:SMF may exert its therapeutic effects on PBC by acting on important targets such as STAT3,JAK2,TNF-α,IL-1β,Vimentin,and E-cadherin and the pathways in which they are involved.展开更多
Swertia mussotii Franch is an endangered medicinal plant in the Qinghai-Tibet Plateau. Its regeneration from callus culture is very difficult. In this study, an efficient method for plant regeneration was developed fr...Swertia mussotii Franch is an endangered medicinal plant in the Qinghai-Tibet Plateau. Its regeneration from callus culture is very difficult. In this study, an efficient method for plant regeneration was developed from its calli. The calli derived from young stem explants of S. mussotii were cultured at two types of temperature treatments to test their efficiency of shoot regeneration. When the calli were cultured at variable temperature (VT, 20℃ during the day and 10℃ at night) treatment, the adventitious shoots were formed at each combination tested. However, that did not occur when the calli were cultured at constant temperature (CT, 25℃) treatment. The best response (53.7%) of plantlet regeneration was obtained on Murashige and Skoog (MS) medium containing 3 mg·l-1 6-benzylaminopurine with 0.5 mg·l-1 α-naphthaleneacetic acid in the VT treatment. The regenerated plantlets were rooted on half-strength MS medium without growth regulators. They flowered in the following subculture. The results indicate that the treatment of day-night temperature difference is a critical factor to callus differentiation in S. mussotii. This protocol can be used for conservation as well as mass propagation of this medicinal plant.展开更多
基金supported by the Key project of Chinese Academy of Sciences(Grant No.ZDRW-ZS-2020-2)Innovation Platform Program of Qinghai Province(2021-ZJ-T02),Key Laboratory Project of Qinghai Province(2022-ZJ-Y05)+1 种基金the Natural Science Foundation of China(Grant No.82171863)China Postdoctoral Science Foundation funded project(2021M701642).
文摘Background:Primary biliary cholangitis(PBC)is a chronic biliary autoimmune liver disease characterized by intrahepatic cholestasis.Swertia mussotii Franch.(SMF)is a Tibetan medicine with hepatoprotective and anti-inflammatory activities.In this study,the therapeutic effect and potential mechanisms of SMF on PBC were investigated by bioinformatics analysis and in vitro experimental validation,with the aim of promoting the progress of SMF and PBC research.Methods:We first explored the therapeutic effects and key targets of SMF on PBC using a network pharmacology approach,further screened the core targets using the GSE79850 dataset,and finally validated the results using molecular docking techniques and in vitro experiments.Results:By bioinformatics analysis,we identified core targets of SMF for PBC treatment(STAT3,JAK2,TNF-α,and IL-1β)and important signaling pathways:JAK-STAT,TNF,and PI3K-AKT.The molecular docking results showed that the significant components of SMF had good binding properties to the core targets.In vitro experiments showed that SMF extracts improved the extent of epithelial-mesenchymal transition in human intrahepatic biliary epithelial cells and had a significant reversal effect on epithelial-mesenchymal transition process markers and potential targets in PBC.Conclusion:SMF may exert its therapeutic effects on PBC by acting on important targets such as STAT3,JAK2,TNF-α,IL-1β,Vimentin,and E-cadherin and the pathways in which they are involved.
文摘Swertia mussotii Franch is an endangered medicinal plant in the Qinghai-Tibet Plateau. Its regeneration from callus culture is very difficult. In this study, an efficient method for plant regeneration was developed from its calli. The calli derived from young stem explants of S. mussotii were cultured at two types of temperature treatments to test their efficiency of shoot regeneration. When the calli were cultured at variable temperature (VT, 20℃ during the day and 10℃ at night) treatment, the adventitious shoots were formed at each combination tested. However, that did not occur when the calli were cultured at constant temperature (CT, 25℃) treatment. The best response (53.7%) of plantlet regeneration was obtained on Murashige and Skoog (MS) medium containing 3 mg·l-1 6-benzylaminopurine with 0.5 mg·l-1 α-naphthaleneacetic acid in the VT treatment. The regenerated plantlets were rooted on half-strength MS medium without growth regulators. They flowered in the following subculture. The results indicate that the treatment of day-night temperature difference is a critical factor to callus differentiation in S. mussotii. This protocol can be used for conservation as well as mass propagation of this medicinal plant.
