目的观察心肌梗死患者入院即时和治疗前心率变异(HRV)的变化,并分析HRV各参数的敏感性。方法采集正常人(正常对照组)、冠心病患者、急性心肌梗死(AMI)患者以及急性心肌缺血动物模型的心电图,进行短时程HRV分析。结果与正常对照组比较,AM...目的观察心肌梗死患者入院即时和治疗前心率变异(HRV)的变化,并分析HRV各参数的敏感性。方法采集正常人(正常对照组)、冠心病患者、急性心肌梗死(AMI)患者以及急性心肌缺血动物模型的心电图,进行短时程HRV分析。结果与正常对照组比较,AMI患者除心率减慢外,其他HRV参数均显著增高,其中反映交感神经兴奋性的窦性心搏RR(N-N)间期的标准差(SDNN)、反映迷走神经张力的相邻RR(N-N)间期差值的标准差(SD of delta NN)、相邻RR(N-N)间期差值>50 ms个数占RR间期总数的百分比(NN50)和poincare散点图短轴参数SD1分别为正常对照组的1.8、2.7、7.5和3.1倍。用垂体后叶素诱发动物产生急性心肌缺血后,HRV的变化也呈类似改变。与正常对照组比较,冠心病患者的HRV各参数减小,其中SDNN和SD of delta NN与正常对照组差异无统计学意义(P>0.05),但SD1则明显减小(P<0.01)。结论 AMI时HRV参数显著增大,慢性稳定型冠心病的HRV参数较正常人减小。用HRV判断自主神经活动变化时,SD1优于其他HRV参数。展开更多
Aim Salvia miltiorrhiza Bunge （SM） and lignum dalbergiae odoriferae （DO） are both traditional Chi- nese medicine that have cardioprotective effects. Here, we further examined the combined effects of SM and DO on r...Aim Salvia miltiorrhiza Bunge （SM） and lignum dalbergiae odoriferae （DO） are both traditional Chi- nese medicine that have cardioprotective effects. Here, we further examined the combined effects of SM and DO on rat myocardial ischemia/reperfusion injury. The possible mechanism of SM and DO also were elucidated. Methods DO was divided into aqueous extract of lignum dalbergiae odoriferae （DOW） and lignum dalbergiae odoriferae oil （DOO）. Sprague-Dawley rats were randomized to seven groups： sham group, model group, treatment groups inclu- ding SM （10 g · kg^-1）, DOW （5 g · kg^-1）, DOO （0.5 ml · kg^-1）, SM ＋ DOW （10 g · kg^-1 ＋ 5 g · kg^-1）, SM ＋ DOO （ 10 g · kg^-1 ＋ 0. 5 ml · kg^-1）. Rats were pretreated with homologous drug for 7 days and then subjec- ted to 30 rain of ischemia followed by 180 rain of reperfusion. Electrocardiogram （ECG） and heart rate were moni- tored and recorded continuously. At the end of reperfusion, blood samples were collected to determine the serum levels of creatine kinase-MB （CK-MB） and lactate dehydrogenase （LDH）. Hearts were harvested to assess heart- body rate, infarct size and histopathological changes as well. Maximum and minimum effective points were deter- mined by measuring indicators associate with myocardial injury at different time-points of reperfusion （Smin, 15min, 30min, 45rain, 60min, 120min, 180min）. The potential therapeutic mechanism of SM and SM ＋ DOO were carried out by detecting superoxide dismutase （SOD）, malondialdehyde （MDA）, tumor necrosis factor-alpha （TNF-alpha） and interleukin 6 （IL-6）. Results The results showed SM and DO can ameliorate cardiac function respectively, and this cardioprotective effect was further strengthened by their combinations. Among all the combi- nations, SM ＋ DOO showed predominant potential to improve ECG and heart rate, reduce heart-body rate （28.5% ＋ 1.4% , P 〈 0.01 vs model） and myocardial infarct size （ 20.96% ＋ 1.61% , P 〈 0.01 vs model, P 〈 0.05 vs SM） , attenuate histopathological damage, decrease the levels of CK-MB and LDH （P 〈 0.01 vs model, P 〈 0.05 vs SM）. The maximum effective points of SM and SM ＋ DOO were 15min and 30rain respectively, and the minimum effective points of them were 180rain. In reducing serum level of MDA, TNF-alpha, IL-6 and increasing SOD activ- ity, SM ＋ DOO was similar to SM. Conclusion The results of this study indicated that SM ＋ DOO have combined effects that are highly effective than single pretreatment against myocardial ischemie reperfusion injury in rats. The possible mechanism of SM and DO were likely through its anti-oxidant and anti-inflammatory properties, and thus may be an effective and promising medicine for both prophylaxis and treatment of ischemic heart disease.展开更多
文摘目的观察心肌梗死患者入院即时和治疗前心率变异(HRV)的变化,并分析HRV各参数的敏感性。方法采集正常人(正常对照组)、冠心病患者、急性心肌梗死(AMI)患者以及急性心肌缺血动物模型的心电图,进行短时程HRV分析。结果与正常对照组比较,AMI患者除心率减慢外,其他HRV参数均显著增高,其中反映交感神经兴奋性的窦性心搏RR(N-N)间期的标准差(SDNN)、反映迷走神经张力的相邻RR(N-N)间期差值的标准差(SD of delta NN)、相邻RR(N-N)间期差值>50 ms个数占RR间期总数的百分比(NN50)和poincare散点图短轴参数SD1分别为正常对照组的1.8、2.7、7.5和3.1倍。用垂体后叶素诱发动物产生急性心肌缺血后,HRV的变化也呈类似改变。与正常对照组比较,冠心病患者的HRV各参数减小,其中SDNN和SD of delta NN与正常对照组差异无统计学意义(P>0.05),但SD1则明显减小(P<0.01)。结论 AMI时HRV参数显著增大,慢性稳定型冠心病的HRV参数较正常人减小。用HRV判断自主神经活动变化时,SD1优于其他HRV参数。
文摘Aim Salvia miltiorrhiza Bunge （SM） and lignum dalbergiae odoriferae （DO） are both traditional Chi- nese medicine that have cardioprotective effects. Here, we further examined the combined effects of SM and DO on rat myocardial ischemia/reperfusion injury. The possible mechanism of SM and DO also were elucidated. Methods DO was divided into aqueous extract of lignum dalbergiae odoriferae （DOW） and lignum dalbergiae odoriferae oil （DOO）. Sprague-Dawley rats were randomized to seven groups： sham group, model group, treatment groups inclu- ding SM （10 g · kg^-1）, DOW （5 g · kg^-1）, DOO （0.5 ml · kg^-1）, SM ＋ DOW （10 g · kg^-1 ＋ 5 g · kg^-1）, SM ＋ DOO （ 10 g · kg^-1 ＋ 0. 5 ml · kg^-1）. Rats were pretreated with homologous drug for 7 days and then subjec- ted to 30 rain of ischemia followed by 180 rain of reperfusion. Electrocardiogram （ECG） and heart rate were moni- tored and recorded continuously. At the end of reperfusion, blood samples were collected to determine the serum levels of creatine kinase-MB （CK-MB） and lactate dehydrogenase （LDH）. Hearts were harvested to assess heart- body rate, infarct size and histopathological changes as well. Maximum and minimum effective points were deter- mined by measuring indicators associate with myocardial injury at different time-points of reperfusion （Smin, 15min, 30min, 45rain, 60min, 120min, 180min）. The potential therapeutic mechanism of SM and SM ＋ DOO were carried out by detecting superoxide dismutase （SOD）, malondialdehyde （MDA）, tumor necrosis factor-alpha （TNF-alpha） and interleukin 6 （IL-6）. Results The results showed SM and DO can ameliorate cardiac function respectively, and this cardioprotective effect was further strengthened by their combinations. Among all the combi- nations, SM ＋ DOO showed predominant potential to improve ECG and heart rate, reduce heart-body rate （28.5% ＋ 1.4% , P 〈 0.01 vs model） and myocardial infarct size （ 20.96% ＋ 1.61% , P 〈 0.01 vs model, P 〈 0.05 vs SM） , attenuate histopathological damage, decrease the levels of CK-MB and LDH （P 〈 0.01 vs model, P 〈 0.05 vs SM）. The maximum effective points of SM and SM ＋ DOO were 15min and 30rain respectively, and the minimum effective points of them were 180rain. In reducing serum level of MDA, TNF-alpha, IL-6 and increasing SOD activ- ity, SM ＋ DOO was similar to SM. Conclusion The results of this study indicated that SM ＋ DOO have combined effects that are highly effective than single pretreatment against myocardial ischemie reperfusion injury in rats. The possible mechanism of SM and DO were likely through its anti-oxidant and anti-inflammatory properties, and thus may be an effective and promising medicine for both prophylaxis and treatment of ischemic heart disease.
