Wolfberry(Lycium barbarum L.)is important for health care and ecological protection.However,it faces problems of low productivity and resource utilization during planting.Exploring reasonable models for water and nitr...Wolfberry(Lycium barbarum L.)is important for health care and ecological protection.However,it faces problems of low productivity and resource utilization during planting.Exploring reasonable models for water and nitrogen management is important for solving these problems.Based on field trials in 2021 and 2022,this study analyzed the effects of controlling soil water and nitrogen application levels on wolfberry height,stem diameter,crown width,yield,and water(WUE)and nitrogen use efficiency(NUE).The upper and lower limits of soil water were controlled by the percentage of soil water content to field water capacity(θ_(f)),and four water levels,i.e.,adequate irrigation(W0,75%-85%θ_(f)),mild water deficit(W1,65%-75%θ_(f)),moderate water deficit(W2,55%-65%θ_(f)),and severe water deficit(W3,45%-55%θ_(f))were used,and three nitrogen application levels,i.e.,no nitrogen(N0,0 kg/hm^(2)),low nitrogen(N1,150 kg/hm^(2)),medium nitrogen(N2,300 kg/hm^(2)),and high nitrogen(N3,450 kg/hm^(2))were implied.The results showed that irrigation and nitrogen application significantly affected plant height,stem diameter,and crown width of wolfberry at different growth stages(P<0.01),and their maximum values were observed in W1N2,W0N2,and W1N3 treatments.Dry weight per plant and yield of wolfberry first increased and then decreased with increasing nitrogen application under the same water treatment.Dry weight per hundred grains and dry weight percentage increased with increasing nitrogen application under W0 treatment.However,under other water treatments,the values first increased and then decreased with increasing nitrogen application.Yield and its component of wolfberry first increased and then decreased as water deficit increased under the same nitrogen treatment.Irrigation water use efficiency(IWUE,8.46 kg/(hm^(2)·mm)),WUE(6.83 kg/(hm^(2)·mm)),partial factor productivity of nitrogen(PFPN,2.56 kg/kg),and NUE(14.29 kg/kg)reached their highest values in W2N2,W1N2,W1N2,and W1N1 treatments.Results of principal component analysis(PCA)showed that yield,WUE,and NUE were better in W1N2 treatment,making it a suitable water and nitrogen management mode for the irrigation area of the Yellow River in the Gansu Province,China and similar planting areas.展开更多
Combined application of chemical fertilizers with organic amendments was recommended as a strategy for improving yield,soil carbon storage,and nutrient use efficiency.However,how the long-term substitution of chemical...Combined application of chemical fertilizers with organic amendments was recommended as a strategy for improving yield,soil carbon storage,and nutrient use efficiency.However,how the long-term substitution of chemical fertilizer with organic manure affects rice yield,carbon sequestration rate(CSR),and nitrogen use efficiency(NUE)while ensuring environmental safety remains unclear.This study assessed the long-term effect of substituting chemical fertilizer with organic manure on rice yield,CSR,and NUE.It also determined the optimum substitution ratio in the acidic soil of southern China.The treatments were:(i)NPK0,unfertilized control;(ii)NPK1,100%chemical nitrogen,phosphorus,and potassium fertilizer;(iii)NPKM1,70%chemical NPK fertilizer and 30%organic manure;(iv)NPKM2,50%chemical NPK fertilizer and 50%organic manure;and(v)NPKM3,30%chemical NPK fertilizer and 70%organic manure.Milk vetch and pig manure were sources of manure for early and late rice seasons,respectively.The result showed that SOC content was higher in NPKM1,NPKM2,and NPKM3 treatments than in NPK0 and NPK1 treatments.The carbon sequestration rate increased by 140,160,and 280%under NPKM1,NPKM2,and NPKM3 treatments,respectively,compared to NPK1 treatment.Grain yield was 86.1,93.1,93.6,and 96.5%higher under NPK1,NPKM1,NPKM2,and NPKM3 treatments,respectively,compared to NPK0 treatment.The NUE in NPKM1,NPKM2,and NPKM3 treatments was higher as compared to NPK1 treatment for both rice seasons.Redundancy analysis revealed close positive relationships of CSR with C input,total N,soil C:N ratio,catalase,and humic acids,whereas NUE was closely related to grain yield,grain N content,and phenol oxidase.Furthermore,CSR and NUE negatively correlated with humin acid and soil C:P and N:P ratios.The technique for order of preference by similarity to ideal solution(TOPSIS)showed that NPKM3 treatment was the optimum strategy for improving CSR and NUE.Therefore,substituting 70%of chemical fertilizer with organic manure could be the best management option for increasing CSR and NUE in the paddy fields of southern China.展开更多
Nitrogen(N) and seeding rates are important factors affecting grain yield and N use efficiency(NUE) in directseeded rice. However, these factors have not been adequately investigated on direct-seeded and double-season...Nitrogen(N) and seeding rates are important factors affecting grain yield and N use efficiency(NUE) in directseeded rice. However, these factors have not been adequately investigated on direct-seeded and double-season rice(DDR) in Central China. The objective of this study was to evaluate the effects of various N and seeding rates on the grain yield and NUE of an ultrashort-duration variety grown under DDR. Field experiments were conducted in 2018 in Wuxue County and 2019 in Qichun County, Hubei Province, China with four N rates and three seeding rates.The results showed that the grain yield of the ultrashort-duration variety ranged from 6.32 to 8.23 t ha–1with a total growth duration of 85 to 97 days across all treatments with N application. Grain yield was increased significantly by N application in most cases, but seeding rate had an inconsistent effect on grain yield. Furthermore, the response of grain yield to the N rates was much higher than the response to seeding rates. The moderate N rates of 100–150 and 70–120 kg N ha–1in the early and late seasons, respectively, could fully express the yield potential of the ultrashort-duration variety grown under DDR. Remarkably higher N responses and agronomic NUE levels were achieved in the early-season rice compared with the late-season rice due to the difference in indigenous soil N supply capacity(INS) between the two seasons. Seasonal differences in INS and N response should be considered when crop management practices are optimized for achieving high grain yield and NUE in ultrashort-duration variety grown under DDR.展开更多
Nitrogen(N)fertilization is necessary for obtaining high rice yield.But excessive N fertilizer reduces rice plant N efficiency and causes negative effects such as environmental pollution.In this study,we assembled key...Nitrogen(N)fertilization is necessary for obtaining high rice yield.But excessive N fertilizer reduces rice plant N efficiency and causes negative effects such as environmental pollution.In this study,we assembled key genes involved in different nodes of N pathways to boost nitrate and ammonium uptake and assimilation,and to strengthen amino acid utilization to increase grain yield and nitrogen use efficiency(NUE)in rice.The combinations OsNPF8.9a×OsNR2,OsAMT1;2×OsGS1;2×OsAS1,and OsGS2×OsAS2×OsANT3 optimized nitrate assimilation,ammonium conversion,and N reutilization,respectively.In co-overexpressing rice lines obtained by co-transformation,the tiller number,biomass,and grain yield per plant of the OsAMT1;2×OsGS1;2×OsAS1-overexpressing line exceeded those of wild-type ZH11,the OsNPF8.9a×OsNR2×OsGS1;2×OsAS1-overexpressing line,and the OsGS2×OsAS2×OsANT3-overexpressing line.The glutamine synthase activity,free amino acids,and nitrogen utilization efficiency(NUt E)of the OsAMT1;2×OsGS1;2×OsAS1-overexpressing line exceeded those of ZH11 and other lines that combined key genes.N influx efficiency was increased in the OsAMT1;2×OsGS1;2×OsAS1-overexpressing line and OsNPF8.9a×OsNR2×OsGS1;2×OsAS1-overexpressing line under a low ammonium and a low nitrate treatment,respectively.We propose that combining overexpression of OsAMT1;2,OsGS1;2,and OsAS1 is a promising breeding strategy for systematically increasing rice grain yield and NUE by focusing on key nodes in the N pathway.展开更多
Utilizing the heterosis of indica/japonica hybrid rice(IJHR)is an effective way to further increase rice grain yield.Rational application of nitrogen(N)fertilizer plays a very important role in using the heterosis of ...Utilizing the heterosis of indica/japonica hybrid rice(IJHR)is an effective way to further increase rice grain yield.Rational application of nitrogen(N)fertilizer plays a very important role in using the heterosis of IJHR to achieve its great yield potential.However,the responses of the grain yield and N utilization of IJHR to N application rates and the underlying physiological mechanism remain elusive.The purpose of this study was to clarify these issues.Three rice cultivars currently used in rice production,an IJHR cultivar Yongyou 2640(YY2640),a japonica cultivar Lianjing 7(LJ-7)and an indica cultivar Yangdao 6(YD-6),were grown in the field with six N rates(0,100,200,300,400,and 500 kg ha^(-1))in 2018 and 2019.The results showed that with the increase in N application rates,the grain yield of each test cultivar increased at first and then decreased,and the highest grain yield was at the N rate of 400 kg ha^(-1)for YY2640,with a grain yield of 13.4 t ha^(-1),and at 300 kg ha^(-1)for LJ-7 and YD-6,with grain yields of 9.4–10.6 t ha^(-1).The grain yield and N use efficiency(NUE)of YY2640 were higher than those of LJ-7 or YD-6 at the same N rate,especially at the higher N rates.When compared with LJ-7 or YD-6,YY2640 exhibited better physiological traits,including greater root oxidation activity and leaf photosynthetic rate,higher cytokinin content in the roots and leaves,and more remobilization of assimilates from the stem to the grain during grain filling.The results suggest that IJHR could attain both higher grain yield and higher NUE than inbred rice at either low or high N application rates.Improved shoot and root traits of the IJHR contribute to its higher grain yield and NUE,and a higher content of cytokinins in the IJHR plants plays a vital role in their responses to N application rates and also benefits other physiological processes.展开更多
Background Nitrate leaching to groundwater and surface water and ammonia volatilization from dairy farms have negative impacts on the environment.Meanwhile,the increasing demand for dairy products will result in more ...Background Nitrate leaching to groundwater and surface water and ammonia volatilization from dairy farms have negative impacts on the environment.Meanwhile,the increasing demand for dairy products will result in more pollution if N losses are not controlled.Therefore,a more efficient,and environmentally friendly production system is needed,in which nitrogen use efficiency(NUE)of dairy cows plays a key role.To genetically improve NUE,extensively recorded and cost-effective proxies are essential,which can be obtained by including mid-infrared(MIR)spectra of milk in prediction models for NUE.This study aimed to develop and validate the best prediction model of NUE,nitrogen loss(NL)and dry matter intake(DMI)for individual dairy cows in China.Results A total of 86 lactating Chinese Holstein cows were used in this study.After data editing,704 records were obtained for calibration and validation.Six prediction models with three different machine learning algorithms and three kinds of pre-processed MIR spectra were developed for each trait.Results showed that the coefficient of determination(R2)of the best model in within-herd validation was 0.66 for NUE,0.58 for NL and 0.63 for DMI.For external validation,reasonable prediction results were only observed for NUE,with R2 ranging from 0.58 to 0.63,while the R2 of the other two traits was below 0.50.The infrared waves from 973.54 to 988.46 cm−1 and daily milk yield were the most important variables for prediction.Conclusion The results showed that individual NUE can be predicted with a moderate accuracy in both within-herd and external validations.The model of NUE could be used for the datasets that are similar to the calibration dataset.The prediction models for NL and 3-day moving average of DMI(DMI_a)generated lower accuracies in within-herd validation.Results also indicated that information of MIR spectra variables increased the predictive ability of models.Additionally,pre-processed MIR spectra do not result in higher accuracy than original MIR spectra in the external validation.These models will be applied to large-scale data to further investigate the genetic architecture of N efficiency and further reduce the adverse impacts on the environment after more data is collected.展开更多
Nitrate(NO_(3)^(-))and ammonium(NH_(4)^(+))are two main inorganic nitrogen(N)sources during crop growth.Here,we enhanced the expression of OsAMT1.1,which encodes a NH_(4)^(+)transporter,using the NO_(3)^(-)-inducible ...Nitrate(NO_(3)^(-))and ammonium(NH_(4)^(+))are two main inorganic nitrogen(N)sources during crop growth.Here,we enhanced the expression of OsAMT1.1,which encodes a NH_(4)^(+)transporter,using the NO_(3)^(-)-inducible promoter of OsNAR2.1 and an ubiquitin promoter in transgenic rice plants.Under field condition of 120 kg/hm2 N,agronomic N use efficiency,N recovery efficiency and N transport efficiency,and grain yield of the pOsNAR2.1:OsAMT1.1 transgenic lines were increased compared with those of the wild type(WT)and the pUbi:OsAMT1.1 transgenic plants.Under 2.0 mmol/L NO_(3)^(-)+0.5 mmol/L NH_(4)^(+)and 0.5 mmol/L NO_(3)^(-)+2.0 mmol/L NH_(4)^(+)conditions of hydroponic culture,compared with the WT,both biomass and total N content were increased in the pOsNAR2.1:OsAMT1.1 transgenic lines.However,biomass was significantly reduced in pUbi:OsAMT1.1 transgenic plants under 0.5 mmol/L NO_(3)^(-)+2.0 mmol/L NH_(4)^(+)condition.The lines expressing pOsNAR2.1:OsAMT1.1 exhibited increased OsAMT1.1 expression and 15NH_(4)^(+)influx in roots under both 2.0 mmol/L NO_(3)^(-)+0.5 mmol/L NH_(4)^(+)and 0.5 mmol/L NO_(3)^(-)+2.0 mmol/L NH_(4)^(+)conditions.Our study showed that expression of OsAMT1.1 can be promoted when driven by the OsNAR2.1 promoter,especially under high-level nitrate condition,leading to enhancement of NH_(4)^(+)uptake,N use efficiency and grain yield.展开更多
Excessive or insufficient application of fertilizer has raised broader concerns regarding soil and environmental degradation.One-time application of slow release fertilizer (SF) has been widely used to reduce yield ga...Excessive or insufficient application of fertilizer has raised broader concerns regarding soil and environmental degradation.One-time application of slow release fertilizer (SF) has been widely used to reduce yield gap with potential maize yield and improve nitrogen use efficiency (NUE).A 2-year field experiment (2018–2019) was conducted to evaluate the effects of SF rates from 0 to 405 kg N ha^(–1) (named F0,SF225,SF270,SF315,SF360,and SF405) and 405 kg N ha^(–1) of common fertilizer(CF405) on the grain yield,biomass and N accumulation,enzymatic activities related with carbon–nitrogen metabolism,NUE and economic analysis.Results indicated that the highest grain yields,NUEs and economic returns were achieved at SF360in both varieties.The enzymatic activities related with carbon–nitrogen metabolism,pre-and post-silking accumulation of biomass and N increased with increasing SF rate,and they were the highest at SF360 and SF405.The grain yield at SF360had no significant difference with that at SF405.However,the N partial factor productivity,N agronomic efficiency and N recovery efficiency at SF360 were 9.8,6.6 and 8.9% higher than that at SF405.The results also indicated that the average grain yields,NUE and economic benefit at SF405 were 5.2,12.3 and 18.1% higher than that at CF405.In conclusion,decreasing N rate from 405 kg ha^(–1)(CF) to 360 kg ha^(–1)(SF) could effectively reduce the yield gap between realized and potential maize yields.The N decreased by 11.1%,but the yield,NUE and economic benefit increased by 3.2,22.2 and 17.5%,which created a simple,efficient and business-friendly system for spring maize production in Jiangsu Province,China.展开更多
To assess the effects of straw return coupled with deep nitrogen(N)fertilization on grain yield and N use efficiency(NUE)in mechanical pot-seedling transplanting(MPST)rice,the seedlings of two rice cultivars,i.e.,Yuxi...To assess the effects of straw return coupled with deep nitrogen(N)fertilization on grain yield and N use efficiency(NUE)in mechanical pot-seedling transplanting(MPST)rice,the seedlings of two rice cultivars,i.e.,Yuxiangyouzhan and Wufengyou 615 transplanted by MPST were applied with N fertilizer at 150 kg/hm2 and straw return at 6 t/hm2 in early seasons of 2019 and 2020.The experiment comprised of following treatments:CK(no fertilizer and no straw return),MDS(deep N fertilization and straw return),MBS(broadcasting fertilizer and straw return),MD(deep N fertilization without straw return),MB(broadcasting fertilizer without straw return).Results depicted that the MDS treatment significantly increased the rice yield by 41.69%-72.22%due to total above-ground biomass,leaf area index and photosynthesis increased by 54.70%-55.80%,38.52%-52.17%and 17.89%-28.40%,respectively,compared to the MB treatment.In addition,the MDS treatment enhanced the total N accumulation by 37.74%-43.69%,N recovery efficiency by 141.45%-164.65%,N agronomic efficiency by 121.76%-134.19%,nitrate reductase by 46.46%-60.86%and glutamine synthetase by 23.56%-31.02%,compared to the MB treatment.The average grain yield and NUE in both years for Yuxiangyouzhan were higher in the MDS treatment than in the MD treatment.Hence,deep N fertilization combined with straw return can be an innovative technique with improved grain yield and NUE in MPST in South China.展开更多
Side deep placement of nitrogen plays an important role in improving rice yield and nitrogen use efficiency.Few studies have examined the effects of reducing the times of nitrogen(RTN)application and reducing the nitr...Side deep placement of nitrogen plays an important role in improving rice yield and nitrogen use efficiency.Few studies have examined the effects of reducing the times of nitrogen(RTN)application and reducing the nitrogen rate(RNR)of application on rice yield and nitrogen use efficiency under side deep placement of nitrogen in paddy fields.Therefore,a field experiment of RNT and RNR treatments was conducted with nine fertilization modes during the 2018–2019 rice growing seasons in a rice–wheat cropping system of the lower reaches of the Yangtze River,China.Rice yield and nitrogen use efficiency were investigated under side deep placement of nitrogen.We found that under the same nitrogen application rate,the yield of RTN3 increased by 9.64 and 10.18%in rice varieties NJ9108 and NJ5718,respectively,compared with the farmers’fertilizer practices(FFP).The nitrogen accumulation of RTN3 was the highest at heading stage,at 11.30 t ha^(–1)across 2018 and 2019.Under the same nitrogen application rate,the N agronomic use efficiency(NAE),N physiological efficiency(NPE)and N recovery efficiency(NRE)of RTN3 were 8.1–21.28%,8.51–41.76%and 0.28–14.52%higher than those of the other fertilization modes,respectively.RNR led to decreases in SPAD value,leaf area index(LAI),dry matter accumulation,nitrogen accumulation,and nitrogen use efficiency.These results suggest that RTN3 increased rice yield and nitrogen use efficiency under the side deep placement of nitrogen,and RNR1 could achieve the goals of saving cost and increasing resource use efficiency.Two fertilization modes RTN3 and RNR1 both could achieve the dual goals of increasing grain yield and resource use efficiency and thus are worth further application and investigation.展开更多
Deep placement of nitrogen fertilizer is a key strategy for improving nitrogen use efficiency. A two-year field experiment was conducted during the early rice growing seasons(March–July) of 2016 and 2017.The experime...Deep placement of nitrogen fertilizer is a key strategy for improving nitrogen use efficiency. A two-year field experiment was conducted during the early rice growing seasons(March–July) of 2016 and 2017.The experimental treatments comprised two rice cultivars: Wufengyou 615(WFY 615) and Yuxiangyouzhan(YXYZ), and three N treatments: mechanical deep placement of all fertilizers as basal dose at 10 cm soil depth(one-time deep-placement fertilization, namely OTDP fertilization);manual surface broadcast(the common farmer practice) of 40% N fertilizer at one day before sowing(basal fertilizer)followed by broadcast application of 30% each at tillering and panicle initiation stages;and no fertilizer application at any growth stage as a control. One-time deep-placement fertilization increased grain yield of both rice cultivars by 11.8%–19.6%, total nitrogen accumulation by 10.3%–13.1%, nitrogen grain production efficiency by 29.7%–31.5%, nitrogen harvest index by 27.8%–30.0%, nitrogen agronomic efficiency by 71.3%–77.2%, and nitrogen recovery efficiency by 42.4%–56.7% for both rice cultivars, compared with the multiple-broadcast treatment. One-time deep-placement fertilization reduced CH4-induced global warming potential(GWP) by 20.7%–25.3%, N2O-induced GWP by 7.2%–12.3%, and total GWP by 14.7%–22.9% for both rice cultivars relative to the multiple-broadcast treatment. The activities of glutamine synthetase and nitrate reductase were increased at both panicle-initiation and heading stages in both rice cultivars following one-time deep-placement fertilization treatment. Larger leaf area index at heading stage and more favorable root morphological traits expressed as larger total root length, mean root diameter, and total root volume per hill were also observed. One-time deep-placement fertilization could be an effective strategy for increasing grain yield and nitrogen use efficiency and lowering greenhouse-gas emissions under mechanical direct-seeded cropping systems.展开更多
In recent years, the use of fertigation technology with center pivot irrigation systems has increased rapidly in the North China Plain (NCP). The combined effects of water and nitrogen application uniformity on the gr...In recent years, the use of fertigation technology with center pivot irrigation systems has increased rapidly in the North China Plain (NCP). The combined effects of water and nitrogen application uniformity on the grain yield, water use efficiency (WUE) and nitrogen use efficiency (NUE) have become a research hotspot. In this study, a two-year field experiment was conducted during the winter wheat growing season in 2016–2018 to evaluate the water application uniformity of a center pivot with two low pressure sprinklers (the R3000 sprinklers were installed in the first span, the corresponding treatment was RS;the D3000 sprinklers were installed in the second span, the corresponding treatment was DS) and a P85A impact sprinkler as the end gun (the corresponding treatment was EG), and to analyze its effects on grain yield, WUE and NUE. The results showed that the water application uniformity coefficients of R3000, D3000 and P85A along the radial direction of the pivot (CUH) were 87.5, 79.5 and 65%, respectively. While the uniformity coefficients along the traveling direction of the pivot (CUC) were all higher than 85%. The effects of water application uniformity of the R3000 and D3000 sprinklers on grain yield were not significant (P>0.05);however, the average grain yield of EG was significantly lower (P<0.05) than those of RS and DS, by 9.4 and 11.1% during two growing seasons, respectively. The coefficients of variation (CV) of the grain yield had a negative correlation with the uniformity coefficient. The CV of WUE was more strongly affected by the water application uniformity, compared with the WUE value, among the three treatments. The NUE of RS was higher than those of DS and EG by about 6.1 and 4.8%, respectively, but there were no significant differences in NUE among the three treatments during the two growing seasons. Although the CUH of the D3000 sprinklers was lower than that of the R3000, it had only limited effects on the grain yield, WUE and NUE. However, the cost of D3000 sprinklers is lower than that of R3000 sprinklers. Therefore, the D3000 sprinklers are recommended for winter wheat irrigation and fertigation in the NCP.展开更多
This paper investigates the yield and nitrogen use efficiency (NUE) of machine-transplanted rice cultivated using mechanized deep placement of N fertilizer in the rice–wheat rotation region of Chuanxi Plain,China.It ...This paper investigates the yield and nitrogen use efficiency (NUE) of machine-transplanted rice cultivated using mechanized deep placement of N fertilizer in the rice–wheat rotation region of Chuanxi Plain,China.It provides theoretical support for N-saving and improves quality and production efficiency of machine-transplanted rice.Using a single-factor complete randomized block design in field experiments in 2018 and 2019,seven N-fertilization treatments were applied,with the fertilizer being surface broadcast and/or mechanically placed beside the seedlings at (5.5±0.5) cm soil depth when transplanting.The treatments were:N0,no N fertilizer;U1,180 kg N ha^(–1) as urea,surface broadcast manually before transplanting;U2,108 kg N ha^(–1) as urea,surface broadcast manually before transplanting,and 72 kg N ha^(–1) as urea surface broadcast manually on the 10th d after transplanting,which is not only the local common fertilization method,but also the reference treatment;UD,180 kg N ha^(–1) as urea,mechanically deep-placed when transplanting;M1,81.6 kg N ha^(–1) as urea and 38.4 kg N ha^(–1) as controlled-release urea (CRU),mechanically deep-placed when transplanting;M2,102 kg N ha^(–1) as urea and48 kg N ha^(–1) as CRU,mechanically deep-placed when transplanting;M3,122.4 kg N ha^(–1) as urea and 57.6 kg N ha^(–1) as CRU,mechanically deep-placed when transplanting.The effects of the N fertilizer treatments on rice yield and NUE were consistent in the 2 yr.With a N application rate of 180 kg ha^(–1),compared with U2,the N recovery efficiency (NRE),N agronomic use efficiency (NAE) and yield under the UD treatment were 20.6,3.5 and 1.1% higher in 2018,and 4.6,1.7 and 1.2% higher in 2019,respectively.Compared with urea alone (U1,U2 or UD),the NRE,NAE and yield achieved by M3 (combined application of urea and controlled-release urea) were higher by 9.2–73.3%,18.6–61.5% and 6.5–16.5%(2018),and 22.2–65.2%,25.6–75.0% and 5.9–13.9%(2019),respectively.Compared with M3,the lower-N treatments M1 and M2 significantly increased NRE by 4.0–7.8% in 2018 and 3.1–4.3% in 2019,respectively.Compared with urea surface application (U1 or U2),the yield under the M2 treatment was higher by 4.3–12.9% in 2018 and 3.6–10.1% in 2019,respectively.Compared with U2,the NRE and NAE under the M2 treatment was higher by 36.9 and 36.3% in 2018,and 33.2 and 37.4% in 2019,mainly because of higher N uptake.There was no significant difference in the concentration of nitrate in the top 0–20 cm soil under U1,U2 and M2 treatments during the full heading and maturity stages.During the full heading stage,U2 produced the highest concentration of nitrite in 0–20 cm and 20–40 cm soil among the N fertilizer treatments.In conclusion,mechanized deep placement of mixed urea and controlled-release urea (M2) at transplanting is a highly-efficient cultivation technology that enables increased yield of machine-transplanted rice and improved NUE,while reducing the amount of N-fertilization applied.展开更多
With increasing population, degrading soil health, limited arable land area, and high cost of nitrogen(N) fertilizers, improving nitrogen use efficiency(NUE) of potato is an inevitable approach to save the environment...With increasing population, degrading soil health, limited arable land area, and high cost of nitrogen(N) fertilizers, improving nitrogen use efficiency(NUE) of potato is an inevitable approach to save the environment and achieve sufficient tuber yields with less N fertilizer supply. Recently, we have developed an aeroponics system to study NUE in potato using genomics, physiology, and breeding approaches. This study aims on precision phenotyping of plants of two distinct potato varieties(Kufri Gaurav, N efficient;Kufri Jyoti, N inefficient) in the novel aeroponics system. Plants were grown in aeroponics under controlled conditions with low N(0.75 mmol L^-1NO3^-) and high N(7.5 mmol L^-1NO3^-) levels. Plant biomass, root traits, total chlorophyll content, and plant N were increased with increasing N supply, whereas higher NUE parameters namely NUE, agronomic NUE(Ag NUE), N uptake efficiency(NUp E), harvest index(HI), and N harvest index(NHI) were observed at low N. An NUE efficient cv. Kufri Gaurav showed higher tuber dry weight, fresh tuber yield, tuber number per plant, early start of tuber harvesting, root traits, stolon traits, NUE parameters, and higher amino acid(aspartic acid and asparagine) content at low N supply. Higher expression of nitrate reductase(NR), nitrite reductase(NIR), and asparagine synthetase(AS) genes was observed in the leaf tissues of Kufri Gaurav at high N. Thus, aeroponics-based precision phenotyping enables identification of NUE efficient genotypes based on key traits and genes involved in improving NUE in potato. Further, this study suggests that the potential of aeroponics can be utilized to investigate N biology in potato under different N regimes.展开更多
The rice cultivars carrying dep1(dense and erect panicle 1)have the potential to achieve both high grain yield and high nitrogen use efficiency(NUE).However,few studies have focused on the agronomic and physiological ...The rice cultivars carrying dep1(dense and erect panicle 1)have the potential to achieve both high grain yield and high nitrogen use efficiency(NUE).However,few studies have focused on the agronomic and physiological performance of those cultivars associated with high yield and high NUE under field conditions.Therefore,we evaluated the yield performance and NUE of two near-isogenic lines(NILs)carrying DEP1(NIL-DEP1)and dep1-1(NIL-dep1)genes under the Nanjing 6 background at 0 and 120 kg N ha^(–1).Grain yield and NUE for grain production(NUEg)were 25.5 and 21.9%higher in NIL-dep1 compared to NIL-DEP1 averaged across N treatments and planting years,respectively.The yield advantage of NIL-dep1 over NIL-DEP1 was mainly due to larger sink size(i.e.,higher total spikelet number),grainfilling percentage,total dry matter production,and harvest index.N utilization rather than N uptake contributed to the high yield of NIL-dep1.Significantly higher NUEg in NIL-dep1 was associated with higher N and dry matter translocation efficiency,lower leaf and stem N concentration at maturity,and higher glutamine synthetase(GS)activity in leaves.In conclusion,dep1 improved grain yield and NUE by increasing N and dry matter transport due to higher leaf GS activity under field conditions during the grain-filling period.展开更多
Controlled-release urea(CRU)has better characteristics than conventional urea for synchronizing nitrogen(N)release with plant uptake.Understanding the effects of CRU on crop yield and N use efficiency(NUE)has long bee...Controlled-release urea(CRU)has better characteristics than conventional urea for synchronizing nitrogen(N)release with plant uptake.Understanding the effects of CRU on crop yield and N use efficiency(NUE)has long been the key to evaluate the performance of CRU.A long-term experiment over five consecutive years was conducted in Changsha,Hunan Province,China,to investigate the effects of polyethylene-coated urea with a 90-d release period on the yield and NUE of double rice(early and late crops are grown in the same year),the amount of residual soil mineral N and the soil–plant N balance,as well as on the economic benefits.Four N fertilizer treatments including CK(no N fertilizer),U(conventional urea),CRU1(polyethylene-coated urea with equal N application rate to U)and CRU2(20%reduction in N application rate of CRU1)were established.The results indicated that CRU1 application increased the yield and NUE of double rice by 11.0 and 13.5%,respectively,compared with U.Higher yield and NUE of late rice were found than in early rice in CRU treatments.Compared with conventional U,the yield and NUE of early rice in the CRU1 treatment were increased by 6.0 and 10.2%,respectively,and those of late rice were increased by 15.4 and 13.8%,respectively.There was no significant difference between CRU1 and CRU2 in double rice yield.Furthermore,CRU treatments(including CRU1 and CRU2)had higher apparent residual Nmin rate(ARNR)and apparent N recovery rate(ANRR),but lower apparent N loss(NS)than the conventional U treatment.Concentrations of NH_(4)^(+)-N and NO_(3)^(-)-N were greater in the surface soil(0–20 cm)and lower in the deeper soil layer(40–60 cm)with CRU treatments than in the U treatment after harvest.Moreover,CRU application produced a greater economic benefit than conventional U application.In general,CRU outperformed U fertilizer in terms of rice yield,NUE,soil–plant N balance,economic benefit,and CRU2 provided greater comprehensive benefits than CRU1.It is suggested that CRU application is beneficial for solving N management challenges in the production of rice.展开更多
Under the limited cultivated land area and the pursuit of sustainable agricultural development,it is essential for the safety of grain production to study agricultural management approaches on narrowing the winter whe...Under the limited cultivated land area and the pursuit of sustainable agricultural development,it is essential for the safety of grain production to study agricultural management approaches on narrowing the winter wheat yield gap and improving nitrogen use efficiency (NUE) in China.In this study,DSSAT-CERES-Wheat Model is used to simulate winter wheat yield under different agricultural treatments,and we analyze yield gaps and NUE with different management scenarios at regional scales and evaluate the suitable approaches for reducing yield gap and increasing NUE.The results show that,the potential of narrowing yield gap ranges 300–900 kg ha^(–1) with soil nutrients increase,400–1 200 kg ha^(–1) with sowing date adjustment and 0–400 kg ha^(–1) with planting density increase as well as 700–2 200 kg ha^(–1) with adding nitrogen fertilizer.Contribution rates of management measures of soil nutrients,sowing date adjusting,planting density,and nitrogen fertilizers are 5–15%,5–15%,0–4%,and 10–20%,respectively.Difference in nitrogen partial productivity ranges 3–10 kg kg^(–1) for soil nutrients,1–10 kg kg^(–1) for sowing date adjusting,1–5 kg kg^(–1) for planting density increase,and–12–0 kg kg^(–1) for adding nitrogen fertilizers,respectively.It indicates that four treatments can narrow yield gap and improve the NUE in varying degrees,but increasing nitrogen fertilizer leads to the decrease of NUE.展开更多
In the present study, appropriate nitrogen(N) application mode in Jianghan Plain was explored by investigating the effects of different N applications on the photosynthetic characteristics of flag leaves and nitrogen ...In the present study, appropriate nitrogen(N) application mode in Jianghan Plain was explored by investigating the effects of different N applications on the photosynthetic characteristics of flag leaves and nitrogen use efficiency(NUE) in a wheat cultivar Zhengmai 9023. Nitrogen was top-dressed before sowing, before winter, and during the jointing stage, at different ratios:1:1:0(N1), 1:0:1(N2), 2:1:1(N3), 1:1:1(N4), and 0:0:1(N5), under the same amount of total N(180 kg/hm^2) during the growing season. No nitrogen fertilizer was used in the control(N0). Results showed that the SPAD values and photosynthetic rate(Pn) of different treatments in flag leaves increased initially and then decreased around the anthesis stage. The two indices in N1 and N5 treatments decreased rapidly after flowering, whereas those in N2, N3, and N4 treatments maintained at high levels for a long period after anthesis. Thus, reasonable nitrogen application could retard the decline of SPAD and Pn after anthesis.N4 and N1 treatments showed large dry matter accumulation. In decreasing order of crop yield, the treatments were: N4 >N1 >N3 >N5 >N2 >N0. The effective panicle number and grain number per spike of N2 were significantly lower than those of other treatments, and there was no significant difference among other treatments. No significant correlation was found between nitrogen application and 1 000-grain weight in this experiment. The nitrogen accumulation, agronomic efficiency of nitrogen fertilizer,nitrogen uptake and use efficiency of above-ground parts, nitrogen uptake and use efficiency of grain of N4 treatment were higher than those of other treatments, but the nitrogen harvest index of N4 was at a low level. In summary, N4 treatment is the most suitable nitrogen application mode in wheat after rice.展开更多
Globally, the current important concern is to minimize nitrogen use in crop culture under climate change condition. The experiment was conducted at Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, B...Globally, the current important concern is to minimize nitrogen use in crop culture under climate change condition. The experiment was conducted at Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh during the rainy season of 2014 to assess growth, yield and nitrogen use efficiency of a new rice variety. The new rice variety (BUdhan 1) was treated with six levels of nitrogenous fertilizer in a randomized complete block design replicated three times. The nitrogen levels 0, 20, 40, 60, 80 and 100 kg N ha<sup>-1</sup> constituted the treatment variables. Results revealed that growth of the new rice variety favored at higher levels of applied nitrogen although it flattened at 80 and 100 kg N <sup>-1</sup>. Preanthesis assimilates reserves contributed to sustaining the yield of the variety which indicated that current photosynthesis was insufficient to support the present yield level. The assimilate remobilization varied from 109.21 to 232.93 g·m<sup>-2</sup> between the nitrogen levels where the maximum amount of remobilization was observed at 60 kg N <sup>-1</sup>. The highest grain yield (5.36 t·<sup>-1</sup>) was found when the variety was fertilized with 60 kg N <sup>-1</sup>. Application of 60 kg N <sup>-1</sup> also showed the highest nitrogen use efficiency (344.50 kg grain/kg N applied) of the variety. We concluded that application of the intermediate level of nitrogen was economical and environment-friendly for the cultivation of new rice variety.展开更多
The crop production in Ethiopia is markedly constrained by soil nutrient depletion and limited fertilizer input. Nitrogen is among the most yield-limiting factors of cereal crops, especially in sub-Saharan Africa (SSA...The crop production in Ethiopia is markedly constrained by soil nutrient depletion and limited fertilizer input. Nitrogen is among the most yield-limiting factors of cereal crops, especially in sub-Saharan Africa (SSA). A meta-analysis of 82 studies was carried out to evaluate the response of major cereal crops, viz. wheat, maize, barley, teff, and sorghum, to nitrogen fertilization in Ethiopia. The results showed that N-application significantly increased yields of all the five crops examined herein. The average yields of the treatment effects over controls for the five crops were 3775.8 kg∙ha<sup>−1</sup> and 2593.3 kg∙ha<sup>−1</sup>, respectively. The overall yield response to nitrogen treatments for all the crops was 64.8% (wheat, 96.5%;maize, 40.65%;barley 84.36%;teff, 50.48%;and sorghum;23%). Overall, nitrogen agronomic efficiency (AE<sub>N</sub>) and partial factor productivity (PFP<sub>N</sub>) were 18.2 and 71.81 kg∙kg<sup>−1</sup>, respectively. A downtrend of nitrogen use efficiency with an increase in N rate was realized. The yield response was higher for the nitrogen treatment effects of >100 kg∙N∙ha<sup>−1</sup> (123.9%), clay soils (75.46%), low initial soil organic carbon (SOC) and available phosphorous (AP) (92.4% and 101.6%), respectively, Therefore, we recommend the application of nitrogen fertilizer (>100 kg∙N∙ha<sup>−1</sup>), especially on infertile soils for improved grain yield and NUE in aforementioned cereal crops in Ethiopia and similar regions in sub-Saharan Africa (SSA).展开更多
基金funded by the National Natural Science Foundation of China(51969003)the Key Research and Development Project of Gansu Province(22YF7NA110)+4 种基金the Discipline Team Construction Project of Gansu Agricultural Universitythe Gansu Agricultural University Youth Mentor Support Fund Project(GAU-QDFC-2022-22)the Innovation Fund Project of Higher Education in Gansu Province(2022B-101)the Research Team Construction Project of College of Water Conservancy and Hydropower Engineering,Gansu Agricultural University(Gaucwky-01)the Gansu Water Science Experimental Research and Technology Extension Program(22GSLK023)。
文摘Wolfberry(Lycium barbarum L.)is important for health care and ecological protection.However,it faces problems of low productivity and resource utilization during planting.Exploring reasonable models for water and nitrogen management is important for solving these problems.Based on field trials in 2021 and 2022,this study analyzed the effects of controlling soil water and nitrogen application levels on wolfberry height,stem diameter,crown width,yield,and water(WUE)and nitrogen use efficiency(NUE).The upper and lower limits of soil water were controlled by the percentage of soil water content to field water capacity(θ_(f)),and four water levels,i.e.,adequate irrigation(W0,75%-85%θ_(f)),mild water deficit(W1,65%-75%θ_(f)),moderate water deficit(W2,55%-65%θ_(f)),and severe water deficit(W3,45%-55%θ_(f))were used,and three nitrogen application levels,i.e.,no nitrogen(N0,0 kg/hm^(2)),low nitrogen(N1,150 kg/hm^(2)),medium nitrogen(N2,300 kg/hm^(2)),and high nitrogen(N3,450 kg/hm^(2))were implied.The results showed that irrigation and nitrogen application significantly affected plant height,stem diameter,and crown width of wolfberry at different growth stages(P<0.01),and their maximum values were observed in W1N2,W0N2,and W1N3 treatments.Dry weight per plant and yield of wolfberry first increased and then decreased with increasing nitrogen application under the same water treatment.Dry weight per hundred grains and dry weight percentage increased with increasing nitrogen application under W0 treatment.However,under other water treatments,the values first increased and then decreased with increasing nitrogen application.Yield and its component of wolfberry first increased and then decreased as water deficit increased under the same nitrogen treatment.Irrigation water use efficiency(IWUE,8.46 kg/(hm^(2)·mm)),WUE(6.83 kg/(hm^(2)·mm)),partial factor productivity of nitrogen(PFPN,2.56 kg/kg),and NUE(14.29 kg/kg)reached their highest values in W2N2,W1N2,W1N2,and W1N1 treatments.Results of principal component analysis(PCA)showed that yield,WUE,and NUE were better in W1N2 treatment,making it a suitable water and nitrogen management mode for the irrigation area of the Yellow River in the Gansu Province,China and similar planting areas.
基金supported by the National Natural Science Foundation of China (41671301)the National Key Research and Development Program of China (2016YFD0300901)the Central Public-interest Scientific Institution Basal Research Fund, China (GY2022-13-5, G2022-02-2, G2022-02-3 and G2022-02-10)
文摘Combined application of chemical fertilizers with organic amendments was recommended as a strategy for improving yield,soil carbon storage,and nutrient use efficiency.However,how the long-term substitution of chemical fertilizer with organic manure affects rice yield,carbon sequestration rate(CSR),and nitrogen use efficiency(NUE)while ensuring environmental safety remains unclear.This study assessed the long-term effect of substituting chemical fertilizer with organic manure on rice yield,CSR,and NUE.It also determined the optimum substitution ratio in the acidic soil of southern China.The treatments were:(i)NPK0,unfertilized control;(ii)NPK1,100%chemical nitrogen,phosphorus,and potassium fertilizer;(iii)NPKM1,70%chemical NPK fertilizer and 30%organic manure;(iv)NPKM2,50%chemical NPK fertilizer and 50%organic manure;and(v)NPKM3,30%chemical NPK fertilizer and 70%organic manure.Milk vetch and pig manure were sources of manure for early and late rice seasons,respectively.The result showed that SOC content was higher in NPKM1,NPKM2,and NPKM3 treatments than in NPK0 and NPK1 treatments.The carbon sequestration rate increased by 140,160,and 280%under NPKM1,NPKM2,and NPKM3 treatments,respectively,compared to NPK1 treatment.Grain yield was 86.1,93.1,93.6,and 96.5%higher under NPK1,NPKM1,NPKM2,and NPKM3 treatments,respectively,compared to NPK0 treatment.The NUE in NPKM1,NPKM2,and NPKM3 treatments was higher as compared to NPK1 treatment for both rice seasons.Redundancy analysis revealed close positive relationships of CSR with C input,total N,soil C:N ratio,catalase,and humic acids,whereas NUE was closely related to grain yield,grain N content,and phenol oxidase.Furthermore,CSR and NUE negatively correlated with humin acid and soil C:P and N:P ratios.The technique for order of preference by similarity to ideal solution(TOPSIS)showed that NPKM3 treatment was the optimum strategy for improving CSR and NUE.Therefore,substituting 70%of chemical fertilizer with organic manure could be the best management option for increasing CSR and NUE in the paddy fields of southern China.
基金supported by the National Natural Science Foundation of China(31971845 and 32101819)the earmarked fund for China Agriculture Research System(CARS-01-20)the China Postdoctoral Science Foundation(2021M691179)。
文摘Nitrogen(N) and seeding rates are important factors affecting grain yield and N use efficiency(NUE) in directseeded rice. However, these factors have not been adequately investigated on direct-seeded and double-season rice(DDR) in Central China. The objective of this study was to evaluate the effects of various N and seeding rates on the grain yield and NUE of an ultrashort-duration variety grown under DDR. Field experiments were conducted in 2018 in Wuxue County and 2019 in Qichun County, Hubei Province, China with four N rates and three seeding rates.The results showed that the grain yield of the ultrashort-duration variety ranged from 6.32 to 8.23 t ha–1with a total growth duration of 85 to 97 days across all treatments with N application. Grain yield was increased significantly by N application in most cases, but seeding rate had an inconsistent effect on grain yield. Furthermore, the response of grain yield to the N rates was much higher than the response to seeding rates. The moderate N rates of 100–150 and 70–120 kg N ha–1in the early and late seasons, respectively, could fully express the yield potential of the ultrashort-duration variety grown under DDR. Remarkably higher N responses and agronomic NUE levels were achieved in the early-season rice compared with the late-season rice due to the difference in indigenous soil N supply capacity(INS) between the two seasons. Seasonal differences in INS and N response should be considered when crop management practices are optimized for achieving high grain yield and NUE in ultrashort-duration variety grown under DDR.
基金supported by the National Natural Science Foundation of China(32260498)the Guizhou Provincial Science and Technology Project(qiankehejichu-ZK(2022)Key 008)+2 种基金the Wuhan Science and Technology Project(2020020601012259)the Guizhou Provincial Science and Technology Support Plan(qiankehezhicheng(2022)Key 026)the Key Cultivation Project of Guizhou University(201903)。
文摘Nitrogen(N)fertilization is necessary for obtaining high rice yield.But excessive N fertilizer reduces rice plant N efficiency and causes negative effects such as environmental pollution.In this study,we assembled key genes involved in different nodes of N pathways to boost nitrate and ammonium uptake and assimilation,and to strengthen amino acid utilization to increase grain yield and nitrogen use efficiency(NUE)in rice.The combinations OsNPF8.9a×OsNR2,OsAMT1;2×OsGS1;2×OsAS1,and OsGS2×OsAS2×OsANT3 optimized nitrate assimilation,ammonium conversion,and N reutilization,respectively.In co-overexpressing rice lines obtained by co-transformation,the tiller number,biomass,and grain yield per plant of the OsAMT1;2×OsGS1;2×OsAS1-overexpressing line exceeded those of wild-type ZH11,the OsNPF8.9a×OsNR2×OsGS1;2×OsAS1-overexpressing line,and the OsGS2×OsAS2×OsANT3-overexpressing line.The glutamine synthase activity,free amino acids,and nitrogen utilization efficiency(NUt E)of the OsAMT1;2×OsGS1;2×OsAS1-overexpressing line exceeded those of ZH11 and other lines that combined key genes.N influx efficiency was increased in the OsAMT1;2×OsGS1;2×OsAS1-overexpressing line and OsNPF8.9a×OsNR2×OsGS1;2×OsAS1-overexpressing line under a low ammonium and a low nitrate treatment,respectively.We propose that combining overexpression of OsAMT1;2,OsGS1;2,and OsAS1 is a promising breeding strategy for systematically increasing rice grain yield and NUE by focusing on key nodes in the N pathway.
基金grateful for grants from the National Natural Science Foundation of China(32071943)the Priority Academic Program Development of Jiangsu Higher Education Institutions,China(PAPD-2020-01)+1 种基金the Postgraduate Research and Innovation Program of Jiangsu Province,China(XKYCX17_052)the Top Talent Supporting Program of Yangzhou University,China(2015-01).
文摘Utilizing the heterosis of indica/japonica hybrid rice(IJHR)is an effective way to further increase rice grain yield.Rational application of nitrogen(N)fertilizer plays a very important role in using the heterosis of IJHR to achieve its great yield potential.However,the responses of the grain yield and N utilization of IJHR to N application rates and the underlying physiological mechanism remain elusive.The purpose of this study was to clarify these issues.Three rice cultivars currently used in rice production,an IJHR cultivar Yongyou 2640(YY2640),a japonica cultivar Lianjing 7(LJ-7)and an indica cultivar Yangdao 6(YD-6),were grown in the field with six N rates(0,100,200,300,400,and 500 kg ha^(-1))in 2018 and 2019.The results showed that with the increase in N application rates,the grain yield of each test cultivar increased at first and then decreased,and the highest grain yield was at the N rate of 400 kg ha^(-1)for YY2640,with a grain yield of 13.4 t ha^(-1),and at 300 kg ha^(-1)for LJ-7 and YD-6,with grain yields of 9.4–10.6 t ha^(-1).The grain yield and N use efficiency(NUE)of YY2640 were higher than those of LJ-7 or YD-6 at the same N rate,especially at the higher N rates.When compared with LJ-7 or YD-6,YY2640 exhibited better physiological traits,including greater root oxidation activity and leaf photosynthetic rate,higher cytokinin content in the roots and leaves,and more remobilization of assimilates from the stem to the grain during grain filling.The results suggest that IJHR could attain both higher grain yield and higher NUE than inbred rice at either low or high N application rates.Improved shoot and root traits of the IJHR contribute to its higher grain yield and NUE,and a higher content of cytokinins in the IJHR plants plays a vital role in their responses to N application rates and also benefits other physiological processes.
基金supported by the earmarked fund for China Agriculture Research System (CARS-36)the Key Research Project of Henan Province (221111111100)+3 种基金the Key Research Project of Ningxia Hui Autonomous Region (2022BBF02017)the Program for Changjiang Scholar and Innovation Research Team in University (IRT_15R62)China Scholarship Council (No.201913043)Hainan University.
文摘Background Nitrate leaching to groundwater and surface water and ammonia volatilization from dairy farms have negative impacts on the environment.Meanwhile,the increasing demand for dairy products will result in more pollution if N losses are not controlled.Therefore,a more efficient,and environmentally friendly production system is needed,in which nitrogen use efficiency(NUE)of dairy cows plays a key role.To genetically improve NUE,extensively recorded and cost-effective proxies are essential,which can be obtained by including mid-infrared(MIR)spectra of milk in prediction models for NUE.This study aimed to develop and validate the best prediction model of NUE,nitrogen loss(NL)and dry matter intake(DMI)for individual dairy cows in China.Results A total of 86 lactating Chinese Holstein cows were used in this study.After data editing,704 records were obtained for calibration and validation.Six prediction models with three different machine learning algorithms and three kinds of pre-processed MIR spectra were developed for each trait.Results showed that the coefficient of determination(R2)of the best model in within-herd validation was 0.66 for NUE,0.58 for NL and 0.63 for DMI.For external validation,reasonable prediction results were only observed for NUE,with R2 ranging from 0.58 to 0.63,while the R2 of the other two traits was below 0.50.The infrared waves from 973.54 to 988.46 cm−1 and daily milk yield were the most important variables for prediction.Conclusion The results showed that individual NUE can be predicted with a moderate accuracy in both within-herd and external validations.The model of NUE could be used for the datasets that are similar to the calibration dataset.The prediction models for NL and 3-day moving average of DMI(DMI_a)generated lower accuracies in within-herd validation.Results also indicated that information of MIR spectra variables increased the predictive ability of models.Additionally,pre-processed MIR spectra do not result in higher accuracy than original MIR spectra in the external validation.These models will be applied to large-scale data to further investigate the genetic architecture of N efficiency and further reduce the adverse impacts on the environment after more data is collected.
基金financially supported by the National Natural Science Foundation of China(Grant No.32061143039)Guangdong Basic and Applied Basic Research Foundation,China(Grant No.2022A1515012381)+1 种基金Shenzhen Science and Technology Program,China(Grant No.JCYJ20210324124409027)the Fundamental Research Funds for the Central Universities,Sun Yat-sen University,China.
文摘Nitrate(NO_(3)^(-))and ammonium(NH_(4)^(+))are two main inorganic nitrogen(N)sources during crop growth.Here,we enhanced the expression of OsAMT1.1,which encodes a NH_(4)^(+)transporter,using the NO_(3)^(-)-inducible promoter of OsNAR2.1 and an ubiquitin promoter in transgenic rice plants.Under field condition of 120 kg/hm2 N,agronomic N use efficiency,N recovery efficiency and N transport efficiency,and grain yield of the pOsNAR2.1:OsAMT1.1 transgenic lines were increased compared with those of the wild type(WT)and the pUbi:OsAMT1.1 transgenic plants.Under 2.0 mmol/L NO_(3)^(-)+0.5 mmol/L NH_(4)^(+)and 0.5 mmol/L NO_(3)^(-)+2.0 mmol/L NH_(4)^(+)conditions of hydroponic culture,compared with the WT,both biomass and total N content were increased in the pOsNAR2.1:OsAMT1.1 transgenic lines.However,biomass was significantly reduced in pUbi:OsAMT1.1 transgenic plants under 0.5 mmol/L NO_(3)^(-)+2.0 mmol/L NH_(4)^(+)condition.The lines expressing pOsNAR2.1:OsAMT1.1 exhibited increased OsAMT1.1 expression and 15NH_(4)^(+)influx in roots under both 2.0 mmol/L NO_(3)^(-)+0.5 mmol/L NH_(4)^(+)and 0.5 mmol/L NO_(3)^(-)+2.0 mmol/L NH_(4)^(+)conditions.Our study showed that expression of OsAMT1.1 can be promoted when driven by the OsNAR2.1 promoter,especially under high-level nitrate condition,leading to enhancement of NH_(4)^(+)uptake,N use efficiency and grain yield.
基金financial support from the National Key Research and Development Program of China(2016YFD0300109)the National Natural Science Foundation of China(31771709)+2 种基金the Jiangsu Agricultural Industry Technology System of China(JATS[2019]458)the High-end Talent Support Program of Yangzhou University,Chinathe Priority Academic Program Development of Jiangsu Higher Education Institutions,China。
文摘Excessive or insufficient application of fertilizer has raised broader concerns regarding soil and environmental degradation.One-time application of slow release fertilizer (SF) has been widely used to reduce yield gap with potential maize yield and improve nitrogen use efficiency (NUE).A 2-year field experiment (2018–2019) was conducted to evaluate the effects of SF rates from 0 to 405 kg N ha^(–1) (named F0,SF225,SF270,SF315,SF360,and SF405) and 405 kg N ha^(–1) of common fertilizer(CF405) on the grain yield,biomass and N accumulation,enzymatic activities related with carbon–nitrogen metabolism,NUE and economic analysis.Results indicated that the highest grain yields,NUEs and economic returns were achieved at SF360in both varieties.The enzymatic activities related with carbon–nitrogen metabolism,pre-and post-silking accumulation of biomass and N increased with increasing SF rate,and they were the highest at SF360 and SF405.The grain yield at SF360had no significant difference with that at SF405.However,the N partial factor productivity,N agronomic efficiency and N recovery efficiency at SF360 were 9.8,6.6 and 8.9% higher than that at SF405.The results also indicated that the average grain yields,NUE and economic benefit at SF405 were 5.2,12.3 and 18.1% higher than that at CF405.In conclusion,decreasing N rate from 405 kg ha^(–1)(CF) to 360 kg ha^(–1)(SF) could effectively reduce the yield gap between realized and potential maize yields.The N decreased by 11.1%,but the yield,NUE and economic benefit increased by 3.2,22.2 and 17.5%,which created a simple,efficient and business-friendly system for spring maize production in Jiangsu Province,China.
基金supported by the Guangdong Basic and Applied Basic Research Foundation,China(Grant No.2021A1515011255)Key-Area Research and Development Program of Guangdong Province,China(Grant No.2019B020221003)National Natural Science Foundation of China(Grant No.31471442)。
文摘To assess the effects of straw return coupled with deep nitrogen(N)fertilization on grain yield and N use efficiency(NUE)in mechanical pot-seedling transplanting(MPST)rice,the seedlings of two rice cultivars,i.e.,Yuxiangyouzhan and Wufengyou 615 transplanted by MPST were applied with N fertilizer at 150 kg/hm2 and straw return at 6 t/hm2 in early seasons of 2019 and 2020.The experiment comprised of following treatments:CK(no fertilizer and no straw return),MDS(deep N fertilization and straw return),MBS(broadcasting fertilizer and straw return),MD(deep N fertilization without straw return),MB(broadcasting fertilizer without straw return).Results depicted that the MDS treatment significantly increased the rice yield by 41.69%-72.22%due to total above-ground biomass,leaf area index and photosynthesis increased by 54.70%-55.80%,38.52%-52.17%and 17.89%-28.40%,respectively,compared to the MB treatment.In addition,the MDS treatment enhanced the total N accumulation by 37.74%-43.69%,N recovery efficiency by 141.45%-164.65%,N agronomic efficiency by 121.76%-134.19%,nitrate reductase by 46.46%-60.86%and glutamine synthetase by 23.56%-31.02%,compared to the MB treatment.The average grain yield and NUE in both years for Yuxiangyouzhan were higher in the MDS treatment than in the MD treatment.Hence,deep N fertilization combined with straw return can be an innovative technique with improved grain yield and NUE in MPST in South China.
基金the National Key Research and Development Program of China(2018YFD0300802 and 2016YFD0200805)the Key Research Program of Jiangsu Province,China(BE2017343 and BE2018362)。
文摘Side deep placement of nitrogen plays an important role in improving rice yield and nitrogen use efficiency.Few studies have examined the effects of reducing the times of nitrogen(RTN)application and reducing the nitrogen rate(RNR)of application on rice yield and nitrogen use efficiency under side deep placement of nitrogen in paddy fields.Therefore,a field experiment of RNT and RNR treatments was conducted with nine fertilization modes during the 2018–2019 rice growing seasons in a rice–wheat cropping system of the lower reaches of the Yangtze River,China.Rice yield and nitrogen use efficiency were investigated under side deep placement of nitrogen.We found that under the same nitrogen application rate,the yield of RTN3 increased by 9.64 and 10.18%in rice varieties NJ9108 and NJ5718,respectively,compared with the farmers’fertilizer practices(FFP).The nitrogen accumulation of RTN3 was the highest at heading stage,at 11.30 t ha^(–1)across 2018 and 2019.Under the same nitrogen application rate,the N agronomic use efficiency(NAE),N physiological efficiency(NPE)and N recovery efficiency(NRE)of RTN3 were 8.1–21.28%,8.51–41.76%and 0.28–14.52%higher than those of the other fertilization modes,respectively.RNR led to decreases in SPAD value,leaf area index(LAI),dry matter accumulation,nitrogen accumulation,and nitrogen use efficiency.These results suggest that RTN3 increased rice yield and nitrogen use efficiency under the side deep placement of nitrogen,and RNR1 could achieve the goals of saving cost and increasing resource use efficiency.Two fertilization modes RTN3 and RNR1 both could achieve the dual goals of increasing grain yield and resource use efficiency and thus are worth further application and investigation.
基金supported by the Key-Area Research and Development Program of Guangdong Province(2019B020221003)National Natural Science Foundation of Guangdong Province(008175187004)National Natural Science Foundation of China(31471442)。
文摘Deep placement of nitrogen fertilizer is a key strategy for improving nitrogen use efficiency. A two-year field experiment was conducted during the early rice growing seasons(March–July) of 2016 and 2017.The experimental treatments comprised two rice cultivars: Wufengyou 615(WFY 615) and Yuxiangyouzhan(YXYZ), and three N treatments: mechanical deep placement of all fertilizers as basal dose at 10 cm soil depth(one-time deep-placement fertilization, namely OTDP fertilization);manual surface broadcast(the common farmer practice) of 40% N fertilizer at one day before sowing(basal fertilizer)followed by broadcast application of 30% each at tillering and panicle initiation stages;and no fertilizer application at any growth stage as a control. One-time deep-placement fertilization increased grain yield of both rice cultivars by 11.8%–19.6%, total nitrogen accumulation by 10.3%–13.1%, nitrogen grain production efficiency by 29.7%–31.5%, nitrogen harvest index by 27.8%–30.0%, nitrogen agronomic efficiency by 71.3%–77.2%, and nitrogen recovery efficiency by 42.4%–56.7% for both rice cultivars, compared with the multiple-broadcast treatment. One-time deep-placement fertilization reduced CH4-induced global warming potential(GWP) by 20.7%–25.3%, N2O-induced GWP by 7.2%–12.3%, and total GWP by 14.7%–22.9% for both rice cultivars relative to the multiple-broadcast treatment. The activities of glutamine synthetase and nitrate reductase were increased at both panicle-initiation and heading stages in both rice cultivars following one-time deep-placement fertilization treatment. Larger leaf area index at heading stage and more favorable root morphological traits expressed as larger total root length, mean root diameter, and total root volume per hill were also observed. One-time deep-placement fertilization could be an effective strategy for increasing grain yield and nitrogen use efficiency and lowering greenhouse-gas emissions under mechanical direct-seeded cropping systems.
基金The research was supported by the National Key Research and Development Program of China(2017YFDO201502)the National Natural Science Foundation of China(51621061 and 51939005)+1 种基金the Science and Technology Open Cooperation Project of Henan Province,China(172106000015)the Open Fund of NationalEngineering Laboratory of Crop Stress Resistance Breeding,China(NELCOF20190104).
文摘In recent years, the use of fertigation technology with center pivot irrigation systems has increased rapidly in the North China Plain (NCP). The combined effects of water and nitrogen application uniformity on the grain yield, water use efficiency (WUE) and nitrogen use efficiency (NUE) have become a research hotspot. In this study, a two-year field experiment was conducted during the winter wheat growing season in 2016–2018 to evaluate the water application uniformity of a center pivot with two low pressure sprinklers (the R3000 sprinklers were installed in the first span, the corresponding treatment was RS;the D3000 sprinklers were installed in the second span, the corresponding treatment was DS) and a P85A impact sprinkler as the end gun (the corresponding treatment was EG), and to analyze its effects on grain yield, WUE and NUE. The results showed that the water application uniformity coefficients of R3000, D3000 and P85A along the radial direction of the pivot (CUH) were 87.5, 79.5 and 65%, respectively. While the uniformity coefficients along the traveling direction of the pivot (CUC) were all higher than 85%. The effects of water application uniformity of the R3000 and D3000 sprinklers on grain yield were not significant (P>0.05);however, the average grain yield of EG was significantly lower (P<0.05) than those of RS and DS, by 9.4 and 11.1% during two growing seasons, respectively. The coefficients of variation (CV) of the grain yield had a negative correlation with the uniformity coefficient. The CV of WUE was more strongly affected by the water application uniformity, compared with the WUE value, among the three treatments. The NUE of RS was higher than those of DS and EG by about 6.1 and 4.8%, respectively, but there were no significant differences in NUE among the three treatments during the two growing seasons. Although the CUH of the D3000 sprinklers was lower than that of the R3000, it had only limited effects on the grain yield, WUE and NUE. However, the cost of D3000 sprinklers is lower than that of R3000 sprinklers. Therefore, the D3000 sprinklers are recommended for winter wheat irrigation and fertigation in the NCP.
基金supported by the National Key Research and Development Program of China(2016YFD0300108)the Application and Basic Research Project of Sichuan Province,China(2018JY0630)the Financial Innovation Capacity Improvement of Sichuan Province,China(2017QNJJ-031)。
文摘This paper investigates the yield and nitrogen use efficiency (NUE) of machine-transplanted rice cultivated using mechanized deep placement of N fertilizer in the rice–wheat rotation region of Chuanxi Plain,China.It provides theoretical support for N-saving and improves quality and production efficiency of machine-transplanted rice.Using a single-factor complete randomized block design in field experiments in 2018 and 2019,seven N-fertilization treatments were applied,with the fertilizer being surface broadcast and/or mechanically placed beside the seedlings at (5.5±0.5) cm soil depth when transplanting.The treatments were:N0,no N fertilizer;U1,180 kg N ha^(–1) as urea,surface broadcast manually before transplanting;U2,108 kg N ha^(–1) as urea,surface broadcast manually before transplanting,and 72 kg N ha^(–1) as urea surface broadcast manually on the 10th d after transplanting,which is not only the local common fertilization method,but also the reference treatment;UD,180 kg N ha^(–1) as urea,mechanically deep-placed when transplanting;M1,81.6 kg N ha^(–1) as urea and 38.4 kg N ha^(–1) as controlled-release urea (CRU),mechanically deep-placed when transplanting;M2,102 kg N ha^(–1) as urea and48 kg N ha^(–1) as CRU,mechanically deep-placed when transplanting;M3,122.4 kg N ha^(–1) as urea and 57.6 kg N ha^(–1) as CRU,mechanically deep-placed when transplanting.The effects of the N fertilizer treatments on rice yield and NUE were consistent in the 2 yr.With a N application rate of 180 kg ha^(–1),compared with U2,the N recovery efficiency (NRE),N agronomic use efficiency (NAE) and yield under the UD treatment were 20.6,3.5 and 1.1% higher in 2018,and 4.6,1.7 and 1.2% higher in 2019,respectively.Compared with urea alone (U1,U2 or UD),the NRE,NAE and yield achieved by M3 (combined application of urea and controlled-release urea) were higher by 9.2–73.3%,18.6–61.5% and 6.5–16.5%(2018),and 22.2–65.2%,25.6–75.0% and 5.9–13.9%(2019),respectively.Compared with M3,the lower-N treatments M1 and M2 significantly increased NRE by 4.0–7.8% in 2018 and 3.1–4.3% in 2019,respectively.Compared with urea surface application (U1 or U2),the yield under the M2 treatment was higher by 4.3–12.9% in 2018 and 3.6–10.1% in 2019,respectively.Compared with U2,the NRE and NAE under the M2 treatment was higher by 36.9 and 36.3% in 2018,and 33.2 and 37.4% in 2019,mainly because of higher N uptake.There was no significant difference in the concentration of nitrate in the top 0–20 cm soil under U1,U2 and M2 treatments during the full heading and maturity stages.During the full heading stage,U2 produced the highest concentration of nitrite in 0–20 cm and 20–40 cm soil among the N fertilizer treatments.In conclusion,mechanized deep placement of mixed urea and controlled-release urea (M2) at transplanting is a highly-efficient cultivation technology that enables increased yield of machine-transplanted rice and improved NUE,while reducing the amount of N-fertilization applied.
基金the Competent Authority, Indian Council of Agricultural Research (ICAR)-Central Potato Research Institute (CPRI), Shimla, Himachal Pradesh, India for necessary supports under the Biotechnology Program and the CABin Scheme (ICAR) (HORTCPRICIL 201500300131)
文摘With increasing population, degrading soil health, limited arable land area, and high cost of nitrogen(N) fertilizers, improving nitrogen use efficiency(NUE) of potato is an inevitable approach to save the environment and achieve sufficient tuber yields with less N fertilizer supply. Recently, we have developed an aeroponics system to study NUE in potato using genomics, physiology, and breeding approaches. This study aims on precision phenotyping of plants of two distinct potato varieties(Kufri Gaurav, N efficient;Kufri Jyoti, N inefficient) in the novel aeroponics system. Plants were grown in aeroponics under controlled conditions with low N(0.75 mmol L^-1NO3^-) and high N(7.5 mmol L^-1NO3^-) levels. Plant biomass, root traits, total chlorophyll content, and plant N were increased with increasing N supply, whereas higher NUE parameters namely NUE, agronomic NUE(Ag NUE), N uptake efficiency(NUp E), harvest index(HI), and N harvest index(NHI) were observed at low N. An NUE efficient cv. Kufri Gaurav showed higher tuber dry weight, fresh tuber yield, tuber number per plant, early start of tuber harvesting, root traits, stolon traits, NUE parameters, and higher amino acid(aspartic acid and asparagine) content at low N supply. Higher expression of nitrate reductase(NR), nitrite reductase(NIR), and asparagine synthetase(AS) genes was observed in the leaf tissues of Kufri Gaurav at high N. Thus, aeroponics-based precision phenotyping enables identification of NUE efficient genotypes based on key traits and genes involved in improving NUE in potato. Further, this study suggests that the potential of aeroponics can be utilized to investigate N biology in potato under different N regimes.
基金supported by the National Natural Science Foundation of China(32071948)the Fundamental Research Funds for the Central Universities,China(2662020ZKPY015)the Guangxi Natural Science Foundation,China(2022GXNSFAA035488)。
文摘The rice cultivars carrying dep1(dense and erect panicle 1)have the potential to achieve both high grain yield and high nitrogen use efficiency(NUE).However,few studies have focused on the agronomic and physiological performance of those cultivars associated with high yield and high NUE under field conditions.Therefore,we evaluated the yield performance and NUE of two near-isogenic lines(NILs)carrying DEP1(NIL-DEP1)and dep1-1(NIL-dep1)genes under the Nanjing 6 background at 0 and 120 kg N ha^(–1).Grain yield and NUE for grain production(NUEg)were 25.5 and 21.9%higher in NIL-dep1 compared to NIL-DEP1 averaged across N treatments and planting years,respectively.The yield advantage of NIL-dep1 over NIL-DEP1 was mainly due to larger sink size(i.e.,higher total spikelet number),grainfilling percentage,total dry matter production,and harvest index.N utilization rather than N uptake contributed to the high yield of NIL-dep1.Significantly higher NUEg in NIL-dep1 was associated with higher N and dry matter translocation efficiency,lower leaf and stem N concentration at maturity,and higher glutamine synthetase(GS)activity in leaves.In conclusion,dep1 improved grain yield and NUE by increasing N and dry matter transport due to higher leaf GS activity under field conditions during the grain-filling period.
基金supported by the National Key R&D Program of China(2017YFD0200703)the National Natural Science Foundation of China(31872177)+2 种基金the Central Public-interest Scientific Institution Basal Research Fund of Chinese Academy of Agricultural Sciences(Y2020XK21)the Natural Science Foundation of Hunan Province,China(2019JJ50233 and 2019JJ50337)the Key Project of Hunan Provincial Education Department,China(20A250)。
文摘Controlled-release urea(CRU)has better characteristics than conventional urea for synchronizing nitrogen(N)release with plant uptake.Understanding the effects of CRU on crop yield and N use efficiency(NUE)has long been the key to evaluate the performance of CRU.A long-term experiment over five consecutive years was conducted in Changsha,Hunan Province,China,to investigate the effects of polyethylene-coated urea with a 90-d release period on the yield and NUE of double rice(early and late crops are grown in the same year),the amount of residual soil mineral N and the soil–plant N balance,as well as on the economic benefits.Four N fertilizer treatments including CK(no N fertilizer),U(conventional urea),CRU1(polyethylene-coated urea with equal N application rate to U)and CRU2(20%reduction in N application rate of CRU1)were established.The results indicated that CRU1 application increased the yield and NUE of double rice by 11.0 and 13.5%,respectively,compared with U.Higher yield and NUE of late rice were found than in early rice in CRU treatments.Compared with conventional U,the yield and NUE of early rice in the CRU1 treatment were increased by 6.0 and 10.2%,respectively,and those of late rice were increased by 15.4 and 13.8%,respectively.There was no significant difference between CRU1 and CRU2 in double rice yield.Furthermore,CRU treatments(including CRU1 and CRU2)had higher apparent residual Nmin rate(ARNR)and apparent N recovery rate(ANRR),but lower apparent N loss(NS)than the conventional U treatment.Concentrations of NH_(4)^(+)-N and NO_(3)^(-)-N were greater in the surface soil(0–20 cm)and lower in the deeper soil layer(40–60 cm)with CRU treatments than in the U treatment after harvest.Moreover,CRU application produced a greater economic benefit than conventional U application.In general,CRU outperformed U fertilizer in terms of rice yield,NUE,soil–plant N balance,economic benefit,and CRU2 provided greater comprehensive benefits than CRU1.It is suggested that CRU application is beneficial for solving N management challenges in the production of rice.
基金financially supported by the National Key Research and Development Program of China(2016YFD0300110,2016YFD0300101)the National Natural Science Foundation of China(31671585,41871253)the Natural Science Foundation of Shandong Province,China(ZR2017ZB0422)。
文摘Under the limited cultivated land area and the pursuit of sustainable agricultural development,it is essential for the safety of grain production to study agricultural management approaches on narrowing the winter wheat yield gap and improving nitrogen use efficiency (NUE) in China.In this study,DSSAT-CERES-Wheat Model is used to simulate winter wheat yield under different agricultural treatments,and we analyze yield gaps and NUE with different management scenarios at regional scales and evaluate the suitable approaches for reducing yield gap and increasing NUE.The results show that,the potential of narrowing yield gap ranges 300–900 kg ha^(–1) with soil nutrients increase,400–1 200 kg ha^(–1) with sowing date adjustment and 0–400 kg ha^(–1) with planting density increase as well as 700–2 200 kg ha^(–1) with adding nitrogen fertilizer.Contribution rates of management measures of soil nutrients,sowing date adjusting,planting density,and nitrogen fertilizers are 5–15%,5–15%,0–4%,and 10–20%,respectively.Difference in nitrogen partial productivity ranges 3–10 kg kg^(–1) for soil nutrients,1–10 kg kg^(–1) for sowing date adjusting,1–5 kg kg^(–1) for planting density increase,and–12–0 kg kg^(–1) for adding nitrogen fertilizers,respectively.It indicates that four treatments can narrow yield gap and improve the NUE in varying degrees,but increasing nitrogen fertilizer leads to the decrease of NUE.
基金Supported by the National Natural Science Foundation of China (31371580)the Key Research and Development Projects of Ministry of Science and Technology of the People’s Republic of China during the 13th-five Year Plan Period (2016YFD0300107)
文摘In the present study, appropriate nitrogen(N) application mode in Jianghan Plain was explored by investigating the effects of different N applications on the photosynthetic characteristics of flag leaves and nitrogen use efficiency(NUE) in a wheat cultivar Zhengmai 9023. Nitrogen was top-dressed before sowing, before winter, and during the jointing stage, at different ratios:1:1:0(N1), 1:0:1(N2), 2:1:1(N3), 1:1:1(N4), and 0:0:1(N5), under the same amount of total N(180 kg/hm^2) during the growing season. No nitrogen fertilizer was used in the control(N0). Results showed that the SPAD values and photosynthetic rate(Pn) of different treatments in flag leaves increased initially and then decreased around the anthesis stage. The two indices in N1 and N5 treatments decreased rapidly after flowering, whereas those in N2, N3, and N4 treatments maintained at high levels for a long period after anthesis. Thus, reasonable nitrogen application could retard the decline of SPAD and Pn after anthesis.N4 and N1 treatments showed large dry matter accumulation. In decreasing order of crop yield, the treatments were: N4 >N1 >N3 >N5 >N2 >N0. The effective panicle number and grain number per spike of N2 were significantly lower than those of other treatments, and there was no significant difference among other treatments. No significant correlation was found between nitrogen application and 1 000-grain weight in this experiment. The nitrogen accumulation, agronomic efficiency of nitrogen fertilizer,nitrogen uptake and use efficiency of above-ground parts, nitrogen uptake and use efficiency of grain of N4 treatment were higher than those of other treatments, but the nitrogen harvest index of N4 was at a low level. In summary, N4 treatment is the most suitable nitrogen application mode in wheat after rice.
文摘Globally, the current important concern is to minimize nitrogen use in crop culture under climate change condition. The experiment was conducted at Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh during the rainy season of 2014 to assess growth, yield and nitrogen use efficiency of a new rice variety. The new rice variety (BUdhan 1) was treated with six levels of nitrogenous fertilizer in a randomized complete block design replicated three times. The nitrogen levels 0, 20, 40, 60, 80 and 100 kg N ha<sup>-1</sup> constituted the treatment variables. Results revealed that growth of the new rice variety favored at higher levels of applied nitrogen although it flattened at 80 and 100 kg N <sup>-1</sup>. Preanthesis assimilates reserves contributed to sustaining the yield of the variety which indicated that current photosynthesis was insufficient to support the present yield level. The assimilate remobilization varied from 109.21 to 232.93 g·m<sup>-2</sup> between the nitrogen levels where the maximum amount of remobilization was observed at 60 kg N <sup>-1</sup>. The highest grain yield (5.36 t·<sup>-1</sup>) was found when the variety was fertilized with 60 kg N <sup>-1</sup>. Application of 60 kg N <sup>-1</sup> also showed the highest nitrogen use efficiency (344.50 kg grain/kg N applied) of the variety. We concluded that application of the intermediate level of nitrogen was economical and environment-friendly for the cultivation of new rice variety.
文摘The crop production in Ethiopia is markedly constrained by soil nutrient depletion and limited fertilizer input. Nitrogen is among the most yield-limiting factors of cereal crops, especially in sub-Saharan Africa (SSA). A meta-analysis of 82 studies was carried out to evaluate the response of major cereal crops, viz. wheat, maize, barley, teff, and sorghum, to nitrogen fertilization in Ethiopia. The results showed that N-application significantly increased yields of all the five crops examined herein. The average yields of the treatment effects over controls for the five crops were 3775.8 kg∙ha<sup>−1</sup> and 2593.3 kg∙ha<sup>−1</sup>, respectively. The overall yield response to nitrogen treatments for all the crops was 64.8% (wheat, 96.5%;maize, 40.65%;barley 84.36%;teff, 50.48%;and sorghum;23%). Overall, nitrogen agronomic efficiency (AE<sub>N</sub>) and partial factor productivity (PFP<sub>N</sub>) were 18.2 and 71.81 kg∙kg<sup>−1</sup>, respectively. A downtrend of nitrogen use efficiency with an increase in N rate was realized. The yield response was higher for the nitrogen treatment effects of >100 kg∙N∙ha<sup>−1</sup> (123.9%), clay soils (75.46%), low initial soil organic carbon (SOC) and available phosphorous (AP) (92.4% and 101.6%), respectively, Therefore, we recommend the application of nitrogen fertilizer (>100 kg∙N∙ha<sup>−1</sup>), especially on infertile soils for improved grain yield and NUE in aforementioned cereal crops in Ethiopia and similar regions in sub-Saharan Africa (SSA).
