The study aimed to discuss baiting effects of 17 kinds of Chinese medicinal plants on Brachydanio rerio.With 100 fish of Brachydanio rerio as tested objects,the average length of the 100 fish was 3.5 cm.Cinnamomum cas...The study aimed to discuss baiting effects of 17 kinds of Chinese medicinal plants on Brachydanio rerio.With 100 fish of Brachydanio rerio as tested objects,the average length of the 100 fish was 3.5 cm.Cinnamomum cassia,Mentha canadensis,Eugenia aromaticum,Coptis chinensis,Phellodendron amurense,Scutellaria baicalensis,star anise,Kaempferia galanga,Prunus mume,Magnolia sinensis,Terminalia chebula meat,Sophora flavescens,Polygonum cuspidatum,Rhizoma polygonatum,Astragalus membranaceus,Forsythia suspense and...展开更多
[ Objective ] The paper was to construct bait vector pGBKTT-Lescpth5, and detect its self-activated transcription activity and the toxicity on yeast cells. [ Method] Lescpth5 was amplified by PCR technique, and connec...[ Objective ] The paper was to construct bait vector pGBKTT-Lescpth5, and detect its self-activated transcription activity and the toxicity on yeast cells. [ Method] Lescpth5 was amplified by PCR technique, and connected into bait vector pGBKTT. The recombined vector was transformed into yeast competent ceils AH109 and carried out self-activated detection and toxicity detection in the au.xotrephic medium. [ Result] Digestion and sequencing results showed that bait vector pGBKT7-Lescpth5 was suecessfully constructed with correct reading frame. Self-activated activity and toxicity detection results showed that bait vector had no self- activated activity on yeast strain AH109, which also had no toxicity on yeast. [Condusion] Bait vector successfully constructed could be used in yeast two-hybrid system, which laid the foundation for screening of cDNA library in the next step.展开更多
基金Supported by National Spark Program(2007EA610021)Tianjin Spark Program(07ZHXHNC05400)~~
文摘The study aimed to discuss baiting effects of 17 kinds of Chinese medicinal plants on Brachydanio rerio.With 100 fish of Brachydanio rerio as tested objects,the average length of the 100 fish was 3.5 cm.Cinnamomum cassia,Mentha canadensis,Eugenia aromaticum,Coptis chinensis,Phellodendron amurense,Scutellaria baicalensis,star anise,Kaempferia galanga,Prunus mume,Magnolia sinensis,Terminalia chebula meat,Sophora flavescens,Polygonum cuspidatum,Rhizoma polygonatum,Astragalus membranaceus,Forsythia suspense and...
基金Supported by Natural Science Foundation of Beijing (6012017)Dr. Funding of Xinjiang Production and Construction Corps~~
文摘[ Objective ] The paper was to construct bait vector pGBKTT-Lescpth5, and detect its self-activated transcription activity and the toxicity on yeast cells. [ Method] Lescpth5 was amplified by PCR technique, and connected into bait vector pGBKTT. The recombined vector was transformed into yeast competent ceils AH109 and carried out self-activated detection and toxicity detection in the au.xotrephic medium. [ Result] Digestion and sequencing results showed that bait vector pGBKT7-Lescpth5 was suecessfully constructed with correct reading frame. Self-activated activity and toxicity detection results showed that bait vector had no self- activated activity on yeast strain AH109, which also had no toxicity on yeast. [Condusion] Bait vector successfully constructed could be used in yeast two-hybrid system, which laid the foundation for screening of cDNA library in the next step.
