Objective:To explore the effect of nootkatone(NKT) on chronic unpredictable mild stress(CUMS)-induced depressive-like behaviors and the mechanism underlying NKT improving the depressivelike behaviors.Methods:The CUMS-...Objective:To explore the effect of nootkatone(NKT) on chronic unpredictable mild stress(CUMS)-induced depressive-like behaviors and the mechanism underlying NKT improving the depressivelike behaviors.Methods:The CUMS-induced depression model was established in mice.Fifty mice were randomized into 5 groups(n=10) in accordance with a random number table:control group,CUMS group,CUMS+NKT(6 mg/kg) group,CUMS+NKT(12 mg/kg) group,and CUMS+ketamine group.From the 22th day,NKT(6 or 12 mg/kg) or ketamine(0.5 mg/kg) was given with intragastric administration every day for21 days.Behavioral tests including forced swimming test(FST),tail suspension test(TST),sucrose preference test(SPT) and open-field test(OFT) were carried out.The mRNA and protein expressions of interleukin(IL)-1β,IL-18,IL-6,and tumor necrosis factor(TNF)-α in hippocampus were assessed using quantitative realtime polymerase chain reaction(PCR),Western blot analysis,and enzyme linked immunosorbent assay.The nuclear factor-κB(NF-κB)/NOD-like receptor 3(NLRP3) inflammasome pathway was analyzed using Western blot and immunofluorescence analysis.Results:NKT treatment improved CUMS-induced depressivelike behaviors in mice(P<0.05 or P<0.01).NKT significantly decreased the mRNA and protein levels of IL-1β,IL-18,IL-6,and TNF-α in hippocampus of CUMS mice(P<0.05 or P<0.01).Furthermore,NKT repressed CUMS-induced activation of NF-κB signaling and NLRP3 inflammasome(P<0.01).More important,Nigericin,a NLRP3 activator,destroyed the effect of NKT on repressing neuroinflammation and improving depressive-like behaviors(P<0.05 or P<0.01).Conclusion:NKT ameliorates the depressive-like symptoms,in part by repressing NF-κB/NLRP3-mediated neuroinflammation.展开更多
Essential oil, with more than thirty kinds of compounds separated and identified by gas chromatography-mass spectrometry, was extracted from Shatian shaddock peel and Sweet shaddock peel by squeeze-steam distillation ...Essential oil, with more than thirty kinds of compounds separated and identified by gas chromatography-mass spectrometry, was extracted from Shatian shaddock peel and Sweet shaddock peel by squeeze-steam distillation and direct steam distillation method. Among their composition, the main components are terpene compounds, which account for 93.926% (mass fraction, the same below) and 85.843% of essential oils extracted from Shatian shaddock peel and Sweet shaddock peel, respectively. Although nootkatone is the major contributor of shaddock characteristic scent, and its contents are 1.069% and 1.749% of essential oils from Sweet shaddock peel and Shatian shaddock peel, respectively. The results show that squeeze-steam distillation gives higher yield and good quality of essential oil and the compositions of essential oils from two kinds of shaddock peels are different, but the main contributors of the shaddock scent are the same.展开更多
基金Supported by the Future Plan of Shanghai Municipal Hospital of Traditional Chinese Medicine (2021) Inheritance Talent Project (No. WLJH2021ZY-MZY033)The Xinglin Inherit-patterned Talents Program of Shanghai University of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine (No. 10 (2019) of the Personnel Office of Shanghai University of TCM)"The Fourth Batch of Clinical Young and Middle-Aged Backbone Teachers’Teaching Ability Improvement Plan".
文摘Objective:To explore the effect of nootkatone(NKT) on chronic unpredictable mild stress(CUMS)-induced depressive-like behaviors and the mechanism underlying NKT improving the depressivelike behaviors.Methods:The CUMS-induced depression model was established in mice.Fifty mice were randomized into 5 groups(n=10) in accordance with a random number table:control group,CUMS group,CUMS+NKT(6 mg/kg) group,CUMS+NKT(12 mg/kg) group,and CUMS+ketamine group.From the 22th day,NKT(6 or 12 mg/kg) or ketamine(0.5 mg/kg) was given with intragastric administration every day for21 days.Behavioral tests including forced swimming test(FST),tail suspension test(TST),sucrose preference test(SPT) and open-field test(OFT) were carried out.The mRNA and protein expressions of interleukin(IL)-1β,IL-18,IL-6,and tumor necrosis factor(TNF)-α in hippocampus were assessed using quantitative realtime polymerase chain reaction(PCR),Western blot analysis,and enzyme linked immunosorbent assay.The nuclear factor-κB(NF-κB)/NOD-like receptor 3(NLRP3) inflammasome pathway was analyzed using Western blot and immunofluorescence analysis.Results:NKT treatment improved CUMS-induced depressivelike behaviors in mice(P<0.05 or P<0.01).NKT significantly decreased the mRNA and protein levels of IL-1β,IL-18,IL-6,and TNF-α in hippocampus of CUMS mice(P<0.05 or P<0.01).Furthermore,NKT repressed CUMS-induced activation of NF-κB signaling and NLRP3 inflammasome(P<0.01).More important,Nigericin,a NLRP3 activator,destroyed the effect of NKT on repressing neuroinflammation and improving depressive-like behaviors(P<0.05 or P<0.01).Conclusion:NKT ameliorates the depressive-like symptoms,in part by repressing NF-κB/NLRP3-mediated neuroinflammation.
文摘Essential oil, with more than thirty kinds of compounds separated and identified by gas chromatography-mass spectrometry, was extracted from Shatian shaddock peel and Sweet shaddock peel by squeeze-steam distillation and direct steam distillation method. Among their composition, the main components are terpene compounds, which account for 93.926% (mass fraction, the same below) and 85.843% of essential oils extracted from Shatian shaddock peel and Sweet shaddock peel, respectively. Although nootkatone is the major contributor of shaddock characteristic scent, and its contents are 1.069% and 1.749% of essential oils from Sweet shaddock peel and Shatian shaddock peel, respectively. The results show that squeeze-steam distillation gives higher yield and good quality of essential oil and the compositions of essential oils from two kinds of shaddock peels are different, but the main contributors of the shaddock scent are the same.
