Mesopelagic fish,the most important daily vertically migrating community in the oceans,are characterized by high lipid content which may obscure the interpretation of stable isotopes analysis.Demersal fish,which are i...Mesopelagic fish,the most important daily vertically migrating community in the oceans,are characterized by high lipid content which may obscure the interpretation of stable isotopes analysis.Demersal fish,which are important consumers in the food web dominated by mesopelagic fish,also have a high lipid content.Here we collected 127 fish samples from the South China Sea and evaluated the effect of lipid contents on△δ^(13)C of mesopelagic and demersal fish.In lipid-extracted mesopelagic fish,the C/N content ratio(<5.5)shows a clear correlation withΔδ^(13)C(the offset of bulk and lipid-extractedδ^(13)C values),especially in non-migratory and semi-migratory species;these values were less correlation in demersal fish.Based on our results,we suggest that mesopelagic and demersal fish in different regions of the South China Sea should be studied separately using appropriate correction models and less fit for the traditional model.Moreover,the C/N content ratio should be used cautiously for establishing the lipid normalization model,especially for the fish in migratory mesopelagic fish and demersal fish.Our results also reveal that mesopelagic fish across nearby regions could be analyzed together.The new models described here can be applied in future studies of mesopelagic and demersal fish in the South China Sea.展开更多
Loquat(Eriobotrya japonica Lindl.)is a subtropical evergreen fruit tree that produces fruits with abundant nutrients and medicinal components.Confirming suitable reference genes for a set of loquat samples before qRT-...Loquat(Eriobotrya japonica Lindl.)is a subtropical evergreen fruit tree that produces fruits with abundant nutrients and medicinal components.Confirming suitable reference genes for a set of loquat samples before qRT-PCR experiments is essential for the accurate quantification of gene expression.In this study,eight candidate reference genes were selected from our previously published RNA-seq data,and primers for each candidate reference gene were designed and evaluated.The Cq values of the candidate reference genes were calculated by RT-qPCR in 31 different loquat samples,including 12 subgroups of developing or abiotic-stressed tissues.Different combinations of stable reference genes were screened according to a comprehensive rank,which was synthesized from the results of four algorithms,including the geNorm,NormFinder,BestKeeper andΔCt methods.The screened reference genes were verified by normalizing EjLGA1 in each subgroup.The obtained suitable combinations of reference genes for accurate normalization were GAPDH,EF1αand ACT for floral development;GAPDH,UBCE and ACT for fruit setting;EF1α,GAPDH and eIF2B for fruit ripening;ACT,EF1αand UBCE for leaves under heat stress;eIF2B,UBCE and EF1αfor leaves under freezing stress;EF1α,TUA and UBCE for leaves under salt stress;ACT,EF1αand eIF2B for immature pulp under freezing stress;ACT,UBCE and eIF2B for immature seeds under freezing stress;EF1α,eIF2B and UBCE for both immature pulp and seeds under freezing stress;UBCE,TUB and TUA for red-fleshed fruits under cold-storage stress;eIF2B,RPS3 and TUB for white-fleshed fruits under coldstorage stress;and eIF2B,UBCE and RPS3 for both red-and white-fleshed fruits under cold-storage stress.This study obtained different combinations of stable reference genes for accurate normalization in twelve subgroups of developing or abiotic-stressed tissues in loquat.To our knowledge,this is the first report to obtain stable reference genes for normalizing gene expression of abiotic-stressed tissues in E.japonica.The use of the three most stable reference genes could increase the reliability of future quantification experiments.展开更多
As an indispensable part of identity authentication,offline writer identification plays a notable role in biology,forensics,and historical document analysis.However,identifying handwriting efficiently,stably,and quick...As an indispensable part of identity authentication,offline writer identification plays a notable role in biology,forensics,and historical document analysis.However,identifying handwriting efficiently,stably,and quickly is still challenging due to the method of extracting and processing handwriting features.In this paper,we propose an efficient system to identify writers through handwritten images,which integrates local and global features from similar handwritten images.The local features are modeled by effective aggregate processing,and global features are extracted through transfer learning.Specifically,the proposed system employs a pre-trained Residual Network to mine the relationship between large image sets and specific handwritten images,while the vector of locally aggregated descriptors with double power normalization is employed in aggregating local and global features.Moreover,handwritten image segmentation,preprocessing,enhancement,optimization of neural network architecture,and normalization for local and global features are exploited,significantly improving system performance.The proposed system is evaluated on Computer Vision Lab(CVL)datasets and the International Conference on Document Analysis and Recognition(ICDAR)2013 datasets.The results show that it represents good generalizability and achieves state-of-the-art performance.Furthermore,the system performs better when training complete handwriting patches with the normalization method.The experimental result indicates that it’s significant to segment handwriting reasonably while dealing with handwriting overlap,which reduces visual burstiness.展开更多
Relative radiometric normalization (RRN) minimizes radiometric differences among images caused by inconsistencies of acquisition conditions rather than changes in surface. Scale invariant feature transform (SIFT) has ...Relative radiometric normalization (RRN) minimizes radiometric differences among images caused by inconsistencies of acquisition conditions rather than changes in surface. Scale invariant feature transform (SIFT) has the ability to automatically extract control points (CPs) and is commonly used for remote sensing images. However, its results are mostly inaccurate and sometimes contain incorrect matching caused by generating a small number of false CP pairs. These CP pairs have high false alarm matching. This paper presents a modified method to improve the performance of SIFT CPs matching by applying sum of absolute difference (SAD) in a different manner for the new optical satellite generation called near-equatorial orbit satellite and multi-sensor images. The proposed method, which has a significantly high rate of correct matches, improves CP matching. The data in this study were obtained from the RazakSAT satellite a new near equatorial satellite system. The proposed method involves six steps: 1) data reduction, 2) applying the SIFT to automatically extract CPs, 3) refining CPs matching by using SAD algorithm with empirical threshold, and 4) calculation of true CPs intensity values over all image’ bands, 5) preforming a linear regression model between the intensity values of CPs locate in reverence and sensed image’ bands, 6) Relative radiometric normalization conducting using regression transformation functions. Different thresholds have experimentally tested and used in conducting this study (50 and 70), by followed the proposed method, and it removed the false extracted SIFT CPs to be from 775, 1125, 883, 804, 883 and 681 false pairs to 342, 424, 547, 706, 547, and 469 corrected and matched pairs, respectively.展开更多
Due to differences in the distribution of scores for different trials, the performance of a speaker verification system will be seriously diminished if raw scores are directly used for detection with a unified thresho...Due to differences in the distribution of scores for different trials, the performance of a speaker verification system will be seriously diminished if raw scores are directly used for detection with a unified threshold value. As such, the scores must be normalized. To tackle the shortcomings of score normalization methods, we propose a speaker verification system based on log-likelihood normalization (LLN). Without a priori knowledge, LLN increases the separation between scores of target and non-target speaker models, so as to improve score aliasing of “same-speaker” and “different-speaker” trials corresponding to the same test speech, enabling better discrimination and decision capability. The experiment shows that LLN is an effective method of scoring normalization.展开更多
针对说话人确认中,各目标话者模型输出评分分布不一致而导致系统确认阈值设置的困难,本文采取了通过评分规整确定系统最小检测代价函数(DCF)确认阈值的方法。在分析了已有的两种评分规整方法Z norm a l-ization和T norm a lization的基...针对说话人确认中,各目标话者模型输出评分分布不一致而导致系统确认阈值设置的困难,本文采取了通过评分规整确定系统最小检测代价函数(DCF)确认阈值的方法。在分析了已有的两种评分规整方法Z norm a l-ization和T norm a lization的基础上,提出了一种结合两者优点的组合规整方法——TZ norm a lization,并据此给出了一种阈值动态修正方法,有效地提高了系统的性能和阈值选取的鲁棒性。对历年的N IST(手机电话语音)评测语料库进行了实验,表明了该方法的有效性。展开更多
针对因特网上数字图像的版权保护、认证和完整性等问题,基于DCT变换、image moment normalization和m序列,提出了一种二值水印嵌入算法,实现了二值图像的嵌入和提取。根据m序列的伪随机性和抗干扰性能,使水印具有良好的隐蔽性和稳健性;...针对因特网上数字图像的版权保护、认证和完整性等问题,基于DCT变换、image moment normalization和m序列,提出了一种二值水印嵌入算法,实现了二值图像的嵌入和提取。根据m序列的伪随机性和抗干扰性能,使水印具有良好的隐蔽性和稳健性;使用了moment normalization能抵制各种几何攻击。实验表明该算法具有很好的鲁棒性、实用性和可操作性。展开更多
-The hydrodynamic coefficients C_d and C_m are not only dependent on the size of slender cylin-der,its location in water,KC number and Re number,but also vary with environmental conditions,i.e.,in regular waves or in ...-The hydrodynamic coefficients C_d and C_m are not only dependent on the size of slender cylin-der,its location in water,KC number and Re number,but also vary with environmental conditions,i.e.,in regular waves or in irregular waves,in pure waves or in wave-current coexisting field.In this paper,thenormalization of hydrodynamic coefficients for various environmental conditions is discussed.When aproper definition of KC number and proper characteristic values of irregular waves are used,a unified re-lationship between C_d,C_m and KC number for regular waves,irregular waves,pure waves and wave-cur-rent coexisting field can be obtained.展开更多
There is a lack of systematic research on the expression of internal control genes used for gene expression normalization in real-time reverse transcription polymerase chain reaction in spinal cord injury research.In ...There is a lack of systematic research on the expression of internal control genes used for gene expression normalization in real-time reverse transcription polymerase chain reaction in spinal cord injury research.In this study,we used rat models of spinal cord hemisection to analyze the expression stability of 13 commonly applied reference genes:Actb,Ankrd27,CypA,Gapdh,Hprt1,Mrpl10,Pgk1,Rictor,Rn18s,Tbp,Ubc,Ubxn11,and Ywhaz.Our results show that the expression of Ankrd27,Ubc,and Tbp were stable after spinal cord injury,while Actb was the most unstable internal control gene.Ankrd27,Ubc,Tbp,and Actb were consequently used to investigate the effects of internal control genes with differing stabilities on the normalization of target gene expression.Target gene expression levels and changes over time were similar when Ankrd27,Ubc,and Tbp were used as internal controls but different when Actb was used as an internal control.We recommend that Ankrd27,Ubc,and Tbp are used as internal control genes for real-time reverse transcription polymerase chain reaction in spinal cord injury research.This study was approved by the Administration Committee of Experimental Animals,Jiangsu Province,China(approval No.20180304-008)on March 4,2018.展开更多
Building detection in very high resolution (VHR) images is crucial for mapping and analysing urban environments. Since buildings are elevated objects, elevation data need to be integrated with images for reliable dete...Building detection in very high resolution (VHR) images is crucial for mapping and analysing urban environments. Since buildings are elevated objects, elevation data need to be integrated with images for reliable detection. This process requires two critical steps: optical-elevation data co-registration and aboveground elevation calculation. These two steps are still challenging to some extent. Therefore, this paper introduces optical-elevation data co-registration and normalization techniques for generating a dataset that facilitates elevation-based building detection. For achieving accurate co-registration, a dense set of stereo-based elevations is generated and co-registered to their relevant image based on their corresponding image locations. To normalize these co-registered elevations, the bare-earth elevations are detected based on classification information of some terrain-level features after achieving the image co-registration. The developed method was executed and validated. After implementation, 80% overall-quality of detection result was achieved with 94% correct detection. Together, the developed techniques successfully facilitate the incorporation of stereo-based elevations for detecting buildings in VHR remote sensing images.展开更多
In this paper, a discussion is devoted to the theory and method of a reversed-frame normalization design to robust flight control system. The robust stability theory of the normal transfer function matrix with the sam...In this paper, a discussion is devoted to the theory and method of a reversed-frame normalization design to robust flight control system. The robust stability theory of the normal transfer function matrix with the same characteristic gain loci is proved. An example of flight control system design shows the application and advantage of this method.展开更多
We report observations in a 30 year old male without structural heart disease who presented with recurrent nocturnal syncope and aborted sudden cardiac death. 12-lead ECG showed elevated ST in inferior leads and short...We report observations in a 30 year old male without structural heart disease who presented with recurrent nocturnal syncope and aborted sudden cardiac death. 12-lead ECG showed elevated ST in inferior leads and short coupled premature ventricular complexes (PVCs). Propafenone challenge suggested a diagnosis of an atypical Brugada syndrome. Two morphological types of PVGs and ventricular fibrillation (VF) were induced during propafenone challenge test. He underwent two ablation procedures in right ventricular inflow tract and left ventricular post-inferior septum region by pace-mapping, respectively. After ablation, VF could not be induced and the elevated ST segments normalized. Two subsequent propafenone challenge tests were also negative. Nonetheless, elevated ST segments and PVCs reappeared by one month follow-up. An implantable defibrillator was recommended, but the patient declined for financial reasons. Unfortunately, he suffered a sudden cardiac death at home 10 weeks post-ablation. These findings suggest that short-term normalization of ventricular repolarizatiion after ablation is possible in Brugada syndrome. However, the transient nature of this finding suggests that it is not a reliable indicator of protection against sudden cardiac death.展开更多
In the era of digital signal processing,like graphics and computation systems,multiplication-accumulation is one of the prime operations.A MAC unit is a vital component of a digital system,like different Fast Fourier ...In the era of digital signal processing,like graphics and computation systems,multiplication-accumulation is one of the prime operations.A MAC unit is a vital component of a digital system,like different Fast Fourier Transform(FFT)algorithms,convolution,image processing algorithms,etcetera.In the domain of digital signal processing,the use of normalization architecture is very vast.The main objective of using normalization is to performcomparison and shift operations.In this research paper,an evolutionary approach for designing an optimized normalization algorithm is proposed using basic logical blocks such as Multiplexer,Adder etc.The proposed normalization algorithm is further used in designing an 8×8 bit Signed Floating-Point Multiply-Accumulate(SFMAC)architecture.Since the SFMAC can accept an 8-bit significand and a 3-bit exponent,the input to the said architecture can be somewhere between−(7.96872)_(10) to+(7.96872)_(10).The proposed architecture is designed and implemented using the Cadence Virtuoso using 90 and 130 nm technologies(in Generic Process Design Kit(GPDK)and Taiwan Semiconductor Manufacturing Company(TSMC),respectively).To reduce the power consumption of the proposed normalization architecture,techniques such as“block enabling”and“clock gating”are used rigorously.According to the analysis done on Cadence,the proposed architecture uses the least amount of power compared to its current predecessors.展开更多
Objective:To assess the stability of 10 candidate internal control genes(ICGs),namely GAPDH,ACTB,RPL23,RPS15A,ATPSF1,GLUT5,HMBS,ATP2B4,PPIA,and BRP to normalize the transcriptional data from testes samples of Zebu and...Objective:To assess the stability of 10 candidate internal control genes(ICGs),namely GAPDH,ACTB,RPL23,RPS15A,ATPSF1,GLUT5,HMBS,ATP2B4,PPIA,and BRP to normalize the transcriptional data from testes samples of Zebu and crossbred bulls.Methods:Total RNA was isolated from testicular tissue of Zebu and crossbred bulls(n=6 each)between 2-8 years of age.cDNA was synthesized,and the quantitative real-time polymerase chain reaction(PCR)was performed.The cycle threshold values were used for the analysis of the stability of ICGs.Four different statistical algorithms:geNorm,Normfinder,BestKeeper,and RefFinder,were used to assess the stability of these genes.Results:ATPSF1,HMBS,PPIA,and RPS15A were the most reliable and stable ICGs for Zebu testes,and ATPSF1,RPL23,and PPIA for crossbred testes.Conclusions:A panel of stable ICGs(ATPSF1,HMBS,PPIA,RPS15A for Zebu and ATPSF1,RPL23,and PPIA for crossbred)for normalization of gene expression data in testes samples can be helpful for researchers to conduct functional genomics studies at the testicular level in cattle bulls.展开更多
Quantitative real-time polymerase chain reaction(qRT-PCR)is a rapid and effective approach toward detecting the expression patterns of target genes.The selection of a stable reference gene under specific test conditio...Quantitative real-time polymerase chain reaction(qRT-PCR)is a rapid and effective approach toward detecting the expression patterns of target genes.The selection of a stable reference gene under specific test condition is essential for expressing levels of target genes accurately.Tilia miqueliana,considered endangered,is a prominent native ornamental and honey tree in East China.No study has evaluated the optimal endogenous reference gene for qRT-PCR analysis in T.miqueliana systematically.In this study,fifteen commonly used reference genes were selected as candidate genes,and the stabilities of their expressions were assessed using four algorithms(GeNorm,NormFiner,BestKeeper,and DeltaCt)in nine experimental datasets.The final integrated evaluation was performed using a comprehensive analysis algorithm(RefFinder).Finally,a target MYB transcription factor gene(TmMYB)was used to verify the accuracy of the candidate reference genes.The results showed that PP2αwas the most stable in tissue set,while RPS13 and SAMCD were optimal for heat and cold stress,respectively.Under waterlogging stress,PP2αand TUB were the most stable genes in the leaves and roots,respectively.EF1αand PP2αwere optimal for drying stress in leaf and root tissues.TUB and ACT7 were the most stable genes in the leaf and root tissues under salt stress.This is the first systematic evaluation of candidate reference genes in T.miqueliana,and it will benefit future studies on expression and functional analysis of target genes in T.miqueliana.展开更多
Nineteen ninety-two is a memorable year in the history of Sino-Japanese rela-tions.It signifies the twentieth anniversary of the normalization of relationsbetween the two countries.Twenty years may seem a mere twinkle...Nineteen ninety-two is a memorable year in the history of Sino-Japanese rela-tions.It signifies the twentieth anniversary of the normalization of relationsbetween the two countries.Twenty years may seem a mere twinkle in history,butthey are of great significance to the Sino-Japanese relations.It is in this period oftime that China and Japan proclaimed the“Joint Statement”,signed the“Sino-展开更多
Massive Multiple Input Multiple Output(MIMO)has been considered as an emerging technology to enhance the spectral and energy efficiency for the upcoming wireless communication systems.This paper derives a closedform a...Massive Multiple Input Multiple Output(MIMO)has been considered as an emerging technology to enhance the spectral and energy efficiency for the upcoming wireless communication systems.This paper derives a closedform approximation for the Ergodic Achievable Secrecy Sum-Rate(EASSR)by considering the joint impact of eavesdroppers and jammers.Two widely used linear precoding techniques,Zero-Forcing(ZF)and Maximum Ratio Transmission(MRT),were used in conjunction with matrix and vector normalization to analyze the secrecy performance.Closed-form expressions are used to explain how the secrecy performance is affected when using the ZF and MRT precoding in the eavesdropping and jamming attack models.We also analyze and compare the performances of different combinations of normalization method and precoding technique in various scenarios.From the analytical expressions and simulation results,we observe that the vector and matrix normalization perform better for the ZF precoding than for the MRT precoding in high Signal-to-Noise Ratio(SNR)scenarios.However,in low SNR,the MRT with matrix normalization outperforms the ZF with vector normalization regardless of the number of users in the system.Further,we observe that the MRT fails to serve more than two users in high SNR scenario.Numerical results obtained from Monte Carlo simulation are used to corroborate the accuracy of the asymptotic secrecy analysis.展开更多
Selection of proper reference genes (RGs) is an essential step needed for accurate normalization of results from genomic studies. Expression of RGs is regulated by many factors such as species, age, gender, type of ti...Selection of proper reference genes (RGs) is an essential step needed for accurate normalization of results from genomic studies. Expression of RGs is regulated by many factors such as species, age, gender, type of tissue, the presence of disease, and the administration of therapeutic treatment. The aim of the present study was to identify optimal RGs in a set of blood samples collected at different time points (0, 24, 48, 72 h) from horses following administration of extracorporeal shock wave therapy (ESWT). The mRNA expression of twelve RGs: HPRT1, ACTB, HSP90A, SDHA, GUSB, B2M, UBC, NONO, TBP, H6PD, RPL32, GAPDH was determined using real time quantitative polymerase chain reaction (qPCR). An SAS program developed on the algorithm of geNorm, SASqPCR, was used to determine stability of the expression and the number of optimal RGs. The results showed that the range of quantification cycle (Cq) values of the evaluated genes varied between 17 and 26 cycles, and that one optimal RG, ACTB, was sufficient for normalization of gene expression. Results of stability of expression demonstrated that ACTB was the optimal choice for all the samples studied. Notably, in samples collected at 72 h post ESWT, TBP showed a significant change in the expression level, and was not suitable for use as a RG. These results substantiate the importance of validating and selecting an appropriate RG.展开更多
In order to improve the condition monitoring and fault diagnosis of wind turbines,a stacked noise reduction autoencoding network based on group normalization is proposed in this paper.The network is based on SCADA dat...In order to improve the condition monitoring and fault diagnosis of wind turbines,a stacked noise reduction autoencoding network based on group normalization is proposed in this paper.The network is based on SCADA data of wind turbine operation,firstly,the group normalization(GN)algorithm is added to solve the problems of stack noise reduction autoencoding network training and slow convergence speed,and the RMSProp algorithm is used to update the weight and the bias of the autoenccoder,which further optimizes the problem that the loss function swings too much during the update process.Finally,in the last layer of the network,the softmax activation function is used to classify the results,and the output of the network is transformed into a probability distribution.The selected wind turbine SCADA data was substituted into the pre-improved and improved stacked denoising autoencoding(SDA)networks for comparative training and verification.The results show that the stacked denoising autoencoding network based on group normalization is more accurate and effective for wind turbine condition monitoring and fault diagnosis,and also provides a reference for wind turbine fault identification.展开更多
基金the National Natural Science Foundation of China under contract Nos 42090043 and 41876074the National Basic Research Program(973 Program)of China under contract No.2014CB441502.
文摘Mesopelagic fish,the most important daily vertically migrating community in the oceans,are characterized by high lipid content which may obscure the interpretation of stable isotopes analysis.Demersal fish,which are important consumers in the food web dominated by mesopelagic fish,also have a high lipid content.Here we collected 127 fish samples from the South China Sea and evaluated the effect of lipid contents on△δ^(13)C of mesopelagic and demersal fish.In lipid-extracted mesopelagic fish,the C/N content ratio(<5.5)shows a clear correlation withΔδ^(13)C(the offset of bulk and lipid-extractedδ^(13)C values),especially in non-migratory and semi-migratory species;these values were less correlation in demersal fish.Based on our results,we suggest that mesopelagic and demersal fish in different regions of the South China Sea should be studied separately using appropriate correction models and less fit for the traditional model.Moreover,the C/N content ratio should be used cautiously for establishing the lipid normalization model,especially for the fish in migratory mesopelagic fish and demersal fish.Our results also reveal that mesopelagic fish across nearby regions could be analyzed together.The new models described here can be applied in future studies of mesopelagic and demersal fish in the South China Sea.
基金funded by the Natural Science Foundation of Fujian Province(2021J05240)Fujian Provincial Science and Technology Project(2021N5014,2022N5006)+1 种基金Research Project of Putian Science and Technology Bureau(2021ZP08,2021ZP09,2021ZP10,2021ZP11)Scientific Research Project of Putian University(2018064).
文摘Loquat(Eriobotrya japonica Lindl.)is a subtropical evergreen fruit tree that produces fruits with abundant nutrients and medicinal components.Confirming suitable reference genes for a set of loquat samples before qRT-PCR experiments is essential for the accurate quantification of gene expression.In this study,eight candidate reference genes were selected from our previously published RNA-seq data,and primers for each candidate reference gene were designed and evaluated.The Cq values of the candidate reference genes were calculated by RT-qPCR in 31 different loquat samples,including 12 subgroups of developing or abiotic-stressed tissues.Different combinations of stable reference genes were screened according to a comprehensive rank,which was synthesized from the results of four algorithms,including the geNorm,NormFinder,BestKeeper andΔCt methods.The screened reference genes were verified by normalizing EjLGA1 in each subgroup.The obtained suitable combinations of reference genes for accurate normalization were GAPDH,EF1αand ACT for floral development;GAPDH,UBCE and ACT for fruit setting;EF1α,GAPDH and eIF2B for fruit ripening;ACT,EF1αand UBCE for leaves under heat stress;eIF2B,UBCE and EF1αfor leaves under freezing stress;EF1α,TUA and UBCE for leaves under salt stress;ACT,EF1αand eIF2B for immature pulp under freezing stress;ACT,UBCE and eIF2B for immature seeds under freezing stress;EF1α,eIF2B and UBCE for both immature pulp and seeds under freezing stress;UBCE,TUB and TUA for red-fleshed fruits under cold-storage stress;eIF2B,RPS3 and TUB for white-fleshed fruits under coldstorage stress;and eIF2B,UBCE and RPS3 for both red-and white-fleshed fruits under cold-storage stress.This study obtained different combinations of stable reference genes for accurate normalization in twelve subgroups of developing or abiotic-stressed tissues in loquat.To our knowledge,this is the first report to obtain stable reference genes for normalizing gene expression of abiotic-stressed tissues in E.japonica.The use of the three most stable reference genes could increase the reliability of future quantification experiments.
基金supported in part by the Postgraduate Research&Practice Innovation Program of Jiangsu Province under Grant KYCX 20_0758in part by the Science and Technology Research Project of Jiangsu Public Security Department under Grant 2020KX005+1 种基金in part by the General Project of Philosophy and Social Science Research in Colleges and Universities in Jiangsu Province under Grant 2022SJYB0473in part by“Cyberspace Security”Construction Project of Jiangsu Provincial Key Discipline during the“14th Five Year Plan”.
文摘As an indispensable part of identity authentication,offline writer identification plays a notable role in biology,forensics,and historical document analysis.However,identifying handwriting efficiently,stably,and quickly is still challenging due to the method of extracting and processing handwriting features.In this paper,we propose an efficient system to identify writers through handwritten images,which integrates local and global features from similar handwritten images.The local features are modeled by effective aggregate processing,and global features are extracted through transfer learning.Specifically,the proposed system employs a pre-trained Residual Network to mine the relationship between large image sets and specific handwritten images,while the vector of locally aggregated descriptors with double power normalization is employed in aggregating local and global features.Moreover,handwritten image segmentation,preprocessing,enhancement,optimization of neural network architecture,and normalization for local and global features are exploited,significantly improving system performance.The proposed system is evaluated on Computer Vision Lab(CVL)datasets and the International Conference on Document Analysis and Recognition(ICDAR)2013 datasets.The results show that it represents good generalizability and achieves state-of-the-art performance.Furthermore,the system performs better when training complete handwriting patches with the normalization method.The experimental result indicates that it’s significant to segment handwriting reasonably while dealing with handwriting overlap,which reduces visual burstiness.
文摘Relative radiometric normalization (RRN) minimizes radiometric differences among images caused by inconsistencies of acquisition conditions rather than changes in surface. Scale invariant feature transform (SIFT) has the ability to automatically extract control points (CPs) and is commonly used for remote sensing images. However, its results are mostly inaccurate and sometimes contain incorrect matching caused by generating a small number of false CP pairs. These CP pairs have high false alarm matching. This paper presents a modified method to improve the performance of SIFT CPs matching by applying sum of absolute difference (SAD) in a different manner for the new optical satellite generation called near-equatorial orbit satellite and multi-sensor images. The proposed method, which has a significantly high rate of correct matches, improves CP matching. The data in this study were obtained from the RazakSAT satellite a new near equatorial satellite system. The proposed method involves six steps: 1) data reduction, 2) applying the SIFT to automatically extract CPs, 3) refining CPs matching by using SAD algorithm with empirical threshold, and 4) calculation of true CPs intensity values over all image’ bands, 5) preforming a linear regression model between the intensity values of CPs locate in reverence and sensed image’ bands, 6) Relative radiometric normalization conducting using regression transformation functions. Different thresholds have experimentally tested and used in conducting this study (50 and 70), by followed the proposed method, and it removed the false extracted SIFT CPs to be from 775, 1125, 883, 804, 883 and 681 false pairs to 342, 424, 547, 706, 547, and 469 corrected and matched pairs, respectively.
文摘Due to differences in the distribution of scores for different trials, the performance of a speaker verification system will be seriously diminished if raw scores are directly used for detection with a unified threshold value. As such, the scores must be normalized. To tackle the shortcomings of score normalization methods, we propose a speaker verification system based on log-likelihood normalization (LLN). Without a priori knowledge, LLN increases the separation between scores of target and non-target speaker models, so as to improve score aliasing of “same-speaker” and “different-speaker” trials corresponding to the same test speech, enabling better discrimination and decision capability. The experiment shows that LLN is an effective method of scoring normalization.
文摘针对说话人确认中,各目标话者模型输出评分分布不一致而导致系统确认阈值设置的困难,本文采取了通过评分规整确定系统最小检测代价函数(DCF)确认阈值的方法。在分析了已有的两种评分规整方法Z norm a l-ization和T norm a lization的基础上,提出了一种结合两者优点的组合规整方法——TZ norm a lization,并据此给出了一种阈值动态修正方法,有效地提高了系统的性能和阈值选取的鲁棒性。对历年的N IST(手机电话语音)评测语料库进行了实验,表明了该方法的有效性。
文摘针对因特网上数字图像的版权保护、认证和完整性等问题,基于DCT变换、image moment normalization和m序列,提出了一种二值水印嵌入算法,实现了二值图像的嵌入和提取。根据m序列的伪随机性和抗干扰性能,使水印具有良好的隐蔽性和稳健性;使用了moment normalization能抵制各种几何攻击。实验表明该算法具有很好的鲁棒性、实用性和可操作性。
基金National Natural Science Foundation of China(No.59779005)
文摘-The hydrodynamic coefficients C_d and C_m are not only dependent on the size of slender cylin-der,its location in water,KC number and Re number,but also vary with environmental conditions,i.e.,in regular waves or in irregular waves,in pure waves or in wave-current coexisting field.In this paper,thenormalization of hydrodynamic coefficients for various environmental conditions is discussed.When aproper definition of KC number and proper characteristic values of irregular waves are used,a unified re-lationship between C_d,C_m and KC number for regular waves,irregular waves,pure waves and wave-cur-rent coexisting field can be obtained.
基金the National Natural Science Foundation of China,No.81901257(to YXW)the Natural Science Foundation of Jiangsu Province of China,No.BK20180951(to YXW)+1 种基金Postgraduate Research and Practice Innovation Program of Jiangsu Province of China,No.KYCX20_2818(to WL)and Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD,to Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education).
文摘There is a lack of systematic research on the expression of internal control genes used for gene expression normalization in real-time reverse transcription polymerase chain reaction in spinal cord injury research.In this study,we used rat models of spinal cord hemisection to analyze the expression stability of 13 commonly applied reference genes:Actb,Ankrd27,CypA,Gapdh,Hprt1,Mrpl10,Pgk1,Rictor,Rn18s,Tbp,Ubc,Ubxn11,and Ywhaz.Our results show that the expression of Ankrd27,Ubc,and Tbp were stable after spinal cord injury,while Actb was the most unstable internal control gene.Ankrd27,Ubc,Tbp,and Actb were consequently used to investigate the effects of internal control genes with differing stabilities on the normalization of target gene expression.Target gene expression levels and changes over time were similar when Ankrd27,Ubc,and Tbp were used as internal controls but different when Actb was used as an internal control.We recommend that Ankrd27,Ubc,and Tbp are used as internal control genes for real-time reverse transcription polymerase chain reaction in spinal cord injury research.This study was approved by the Administration Committee of Experimental Animals,Jiangsu Province,China(approval No.20180304-008)on March 4,2018.
文摘Building detection in very high resolution (VHR) images is crucial for mapping and analysing urban environments. Since buildings are elevated objects, elevation data need to be integrated with images for reliable detection. This process requires two critical steps: optical-elevation data co-registration and aboveground elevation calculation. These two steps are still challenging to some extent. Therefore, this paper introduces optical-elevation data co-registration and normalization techniques for generating a dataset that facilitates elevation-based building detection. For achieving accurate co-registration, a dense set of stereo-based elevations is generated and co-registered to their relevant image based on their corresponding image locations. To normalize these co-registered elevations, the bare-earth elevations are detected based on classification information of some terrain-level features after achieving the image co-registration. The developed method was executed and validated. After implementation, 80% overall-quality of detection result was achieved with 94% correct detection. Together, the developed techniques successfully facilitate the incorporation of stereo-based elevations for detecting buildings in VHR remote sensing images.
基金This paper has been supported by the National Natural Science Foundation of Chaina.
文摘In this paper, a discussion is devoted to the theory and method of a reversed-frame normalization design to robust flight control system. The robust stability theory of the normal transfer function matrix with the same characteristic gain loci is proved. An example of flight control system design shows the application and advantage of this method.
文摘We report observations in a 30 year old male without structural heart disease who presented with recurrent nocturnal syncope and aborted sudden cardiac death. 12-lead ECG showed elevated ST in inferior leads and short coupled premature ventricular complexes (PVCs). Propafenone challenge suggested a diagnosis of an atypical Brugada syndrome. Two morphological types of PVGs and ventricular fibrillation (VF) were induced during propafenone challenge test. He underwent two ablation procedures in right ventricular inflow tract and left ventricular post-inferior septum region by pace-mapping, respectively. After ablation, VF could not be induced and the elevated ST segments normalized. Two subsequent propafenone challenge tests were also negative. Nonetheless, elevated ST segments and PVCs reappeared by one month follow-up. An implantable defibrillator was recommended, but the patient declined for financial reasons. Unfortunately, he suffered a sudden cardiac death at home 10 weeks post-ablation. These findings suggest that short-term normalization of ventricular repolarizatiion after ablation is possible in Brugada syndrome. However, the transient nature of this finding suggests that it is not a reliable indicator of protection against sudden cardiac death.
基金This work was supported by Research Support Fund(RSF)of Symbiosis International(Deemed University),Pune,India。
文摘In the era of digital signal processing,like graphics and computation systems,multiplication-accumulation is one of the prime operations.A MAC unit is a vital component of a digital system,like different Fast Fourier Transform(FFT)algorithms,convolution,image processing algorithms,etcetera.In the domain of digital signal processing,the use of normalization architecture is very vast.The main objective of using normalization is to performcomparison and shift operations.In this research paper,an evolutionary approach for designing an optimized normalization algorithm is proposed using basic logical blocks such as Multiplexer,Adder etc.The proposed normalization algorithm is further used in designing an 8×8 bit Signed Floating-Point Multiply-Accumulate(SFMAC)architecture.Since the SFMAC can accept an 8-bit significand and a 3-bit exponent,the input to the said architecture can be somewhere between−(7.96872)_(10) to+(7.96872)_(10).The proposed architecture is designed and implemented using the Cadence Virtuoso using 90 and 130 nm technologies(in Generic Process Design Kit(GPDK)and Taiwan Semiconductor Manufacturing Company(TSMC),respectively).To reduce the power consumption of the proposed normalization architecture,techniques such as“block enabling”and“clock gating”are used rigorously.According to the analysis done on Cadence,the proposed architecture uses the least amount of power compared to its current predecessors.
基金The present study was carried out under the project“Molecular markers for improving reproduction in cattle and buffaloes”under the funding of Bill and Melinda Gates Foundation,USA and Indian Council of Agricultural Research-National Dairy Research Institute.
文摘Objective:To assess the stability of 10 candidate internal control genes(ICGs),namely GAPDH,ACTB,RPL23,RPS15A,ATPSF1,GLUT5,HMBS,ATP2B4,PPIA,and BRP to normalize the transcriptional data from testes samples of Zebu and crossbred bulls.Methods:Total RNA was isolated from testicular tissue of Zebu and crossbred bulls(n=6 each)between 2-8 years of age.cDNA was synthesized,and the quantitative real-time polymerase chain reaction(PCR)was performed.The cycle threshold values were used for the analysis of the stability of ICGs.Four different statistical algorithms:geNorm,Normfinder,BestKeeper,and RefFinder,were used to assess the stability of these genes.Results:ATPSF1,HMBS,PPIA,and RPS15A were the most reliable and stable ICGs for Zebu testes,and ATPSF1,RPL23,and PPIA for crossbred testes.Conclusions:A panel of stable ICGs(ATPSF1,HMBS,PPIA,RPS15A for Zebu and ATPSF1,RPL23,and PPIA for crossbred)for normalization of gene expression data in testes samples can be helpful for researchers to conduct functional genomics studies at the testicular level in cattle bulls.
基金the Natural Science Foundation of Jiangsu Province under Grant No.BK20170619National Natural Science Foundation of China under Grant No.31700477Innovation and Extension of Forestry Science and Technology of Jiangsu Province under Grant No.LYKJ[2019]06.
文摘Quantitative real-time polymerase chain reaction(qRT-PCR)is a rapid and effective approach toward detecting the expression patterns of target genes.The selection of a stable reference gene under specific test condition is essential for expressing levels of target genes accurately.Tilia miqueliana,considered endangered,is a prominent native ornamental and honey tree in East China.No study has evaluated the optimal endogenous reference gene for qRT-PCR analysis in T.miqueliana systematically.In this study,fifteen commonly used reference genes were selected as candidate genes,and the stabilities of their expressions were assessed using four algorithms(GeNorm,NormFiner,BestKeeper,and DeltaCt)in nine experimental datasets.The final integrated evaluation was performed using a comprehensive analysis algorithm(RefFinder).Finally,a target MYB transcription factor gene(TmMYB)was used to verify the accuracy of the candidate reference genes.The results showed that PP2αwas the most stable in tissue set,while RPS13 and SAMCD were optimal for heat and cold stress,respectively.Under waterlogging stress,PP2αand TUB were the most stable genes in the leaves and roots,respectively.EF1αand PP2αwere optimal for drying stress in leaf and root tissues.TUB and ACT7 were the most stable genes in the leaf and root tissues under salt stress.This is the first systematic evaluation of candidate reference genes in T.miqueliana,and it will benefit future studies on expression and functional analysis of target genes in T.miqueliana.
文摘Nineteen ninety-two is a memorable year in the history of Sino-Japanese rela-tions.It signifies the twentieth anniversary of the normalization of relationsbetween the two countries.Twenty years may seem a mere twinkle in history,butthey are of great significance to the Sino-Japanese relations.It is in this period oftime that China and Japan proclaimed the“Joint Statement”,signed the“Sino-
文摘Massive Multiple Input Multiple Output(MIMO)has been considered as an emerging technology to enhance the spectral and energy efficiency for the upcoming wireless communication systems.This paper derives a closedform approximation for the Ergodic Achievable Secrecy Sum-Rate(EASSR)by considering the joint impact of eavesdroppers and jammers.Two widely used linear precoding techniques,Zero-Forcing(ZF)and Maximum Ratio Transmission(MRT),were used in conjunction with matrix and vector normalization to analyze the secrecy performance.Closed-form expressions are used to explain how the secrecy performance is affected when using the ZF and MRT precoding in the eavesdropping and jamming attack models.We also analyze and compare the performances of different combinations of normalization method and precoding technique in various scenarios.From the analytical expressions and simulation results,we observe that the vector and matrix normalization perform better for the ZF precoding than for the MRT precoding in high Signal-to-Noise Ratio(SNR)scenarios.However,in low SNR,the MRT with matrix normalization outperforms the ZF with vector normalization regardless of the number of users in the system.Further,we observe that the MRT fails to serve more than two users in high SNR scenario.Numerical results obtained from Monte Carlo simulation are used to corroborate the accuracy of the asymptotic secrecy analysis.
文摘Selection of proper reference genes (RGs) is an essential step needed for accurate normalization of results from genomic studies. Expression of RGs is regulated by many factors such as species, age, gender, type of tissue, the presence of disease, and the administration of therapeutic treatment. The aim of the present study was to identify optimal RGs in a set of blood samples collected at different time points (0, 24, 48, 72 h) from horses following administration of extracorporeal shock wave therapy (ESWT). The mRNA expression of twelve RGs: HPRT1, ACTB, HSP90A, SDHA, GUSB, B2M, UBC, NONO, TBP, H6PD, RPL32, GAPDH was determined using real time quantitative polymerase chain reaction (qPCR). An SAS program developed on the algorithm of geNorm, SASqPCR, was used to determine stability of the expression and the number of optimal RGs. The results showed that the range of quantification cycle (Cq) values of the evaluated genes varied between 17 and 26 cycles, and that one optimal RG, ACTB, was sufficient for normalization of gene expression. Results of stability of expression demonstrated that ACTB was the optimal choice for all the samples studied. Notably, in samples collected at 72 h post ESWT, TBP showed a significant change in the expression level, and was not suitable for use as a RG. These results substantiate the importance of validating and selecting an appropriate RG.
基金the National Natural Science Foundation of China(51767014),2018–2021.
文摘In order to improve the condition monitoring and fault diagnosis of wind turbines,a stacked noise reduction autoencoding network based on group normalization is proposed in this paper.The network is based on SCADA data of wind turbine operation,firstly,the group normalization(GN)algorithm is added to solve the problems of stack noise reduction autoencoding network training and slow convergence speed,and the RMSProp algorithm is used to update the weight and the bias of the autoenccoder,which further optimizes the problem that the loss function swings too much during the update process.Finally,in the last layer of the network,the softmax activation function is used to classify the results,and the output of the network is transformed into a probability distribution.The selected wind turbine SCADA data was substituted into the pre-improved and improved stacked denoising autoencoding(SDA)networks for comparative training and verification.The results show that the stacked denoising autoencoding network based on group normalization is more accurate and effective for wind turbine condition monitoring and fault diagnosis,and also provides a reference for wind turbine fault identification.
