Cymbidium goeringii is an economically important ornamental plant,and flower color is one of the main features of C.goeringii that contributes to its high economic value.To clarify the molecular mechanisms underlying ...Cymbidium goeringii is an economically important ornamental plant,and flower color is one of the main features of C.goeringii that contributes to its high economic value.To clarify the molecular mechanisms underlying the role of anthocyanins in mediating differences in color among varieties,liquid chromatography–tandem mass spectrometry was used to perform anthocyanin-targeted metabolomics of seven C.goeringii varieties,including‘Jin Qian Yuan’(JQY),‘Jin Xiu Qian Yuan’(JXQY),‘Miao Jiang Su Die’(MJSD),‘Qian Ming Su’(QMS),‘Shi Chan’(SC),and‘Yang Ming Su’(YMS),as well as the C.goeringii.We detected 64 anthocyanins,including cyanidins,delphinidins,malvidins,pelargonidins,peonidins,petunidins,procyanidins,and flavonoids.We identified six shared differentially accumulated metabolites(DAMs),including cyanidin-3-O-rutinoside,delphinidin-3-Osophoroside,pelargonidin-3-O-rutinoside,peonidin-3-O-(6-O-malonyl-beta-D-glucoside),peonidin-3-Osophoroside,and chalcone.Most DAMs were enriched in the anthocyanin biosynthesis pathway.Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed that the differentially expressed metabolites were significantly enriched in the anthocyanin biosynthesis pathway.Analysis of the content of differentially expressed metabolites indicated that peonidin-3-O-(6-O-malonyl-beta-D-glucoside)was the key metabolite underlying color differences among C.goeringii varieties.Procyanidin B2,pelargonidin-3-O-galactoside,and naringenin might also affect the color formation of JQY and QMS,SC,and MJSD,respectively.The results of this study shed light on the metabolic mechanism underlying flower color differences in C.goeringii at the molecular level.Our findings will aid future studies of the mechanism of flower color regulation in C.goeringii and have implications for the breeding of new varieties.展开更多
DNA methylation is an important epigenetic regulatory mechanism,it regulates gene expression by recruiting proteins involved in gene repression or by inhibiting the binding of transcription factor(s)to DNA.In this stu...DNA methylation is an important epigenetic regulatory mechanism,it regulates gene expression by recruiting proteins involved in gene repression or by inhibiting the binding of transcription factor(s)to DNA.In this study,a novel methyltransferase 2a gene(Zmet2a)was cloned in maize and identified by polymerase chain reaction-base(PCR-base)using a bioinformatics strategy.The Zmet2a cDNA sequence is 2739 bp long and translates to 912 amino acid peptides.The Zmet2a protein revealed that it contains BAH and CHROMO structural domains,is a non-transmembrane protein that is hydrophilically unstable,and has no signal peptide structure.Meanwhile,we verified the biological roles of Zmet2a using transgenic Arabidopsis overexpressing Zmet2a and Zmet2a-knockout maize.Transgenic Zmet2a Arabidopsis thaliana showed highly significant advancement inflowering time,and Zmet2a-knockout maize showed advancement inflowering time,with significant changes in several traits.Altogether,these report the role of Zmet2a in the regulation offlowering time,which will lay a foundation for revealing the biological function and epigenetic regulation mechanism of Zmet2a in the growth,development andflowering of maize.展开更多
Aim:The present study was conducted to investigate the protective effect of chamomile fl owers methanolic extract(CFME)and fennel seeds methanolic extract(FSME)on azathioprine(AZA),an immunosuppressant drug,which indu...Aim:The present study was conducted to investigate the protective effect of chamomile fl owers methanolic extract(CFME)and fennel seeds methanolic extract(FSME)on azathioprine(AZA),an immunosuppressant drug,which induced a liver injury and oxidative stress in rats.Methods:Rats were divided into 6 groups(8 rats each)and treated orally for 28 consecutive days as follows.Group 1:rats were given normal saline and used as controls;group 2:rats treated with CFME(200 mg/kg);group 3:rats treated with FSME(200 mg/kg);group 4:rats treated with AZA(25 mg/kg);and groups 5 and 6:rats treated with CFME(200 mg/kg)or FSME(200 mg/kg)15 min prior to AZA(25 mg/kg)treatment.At the end of experimental period,blood and liver samples were collected from all groups for biochemical analysis and histological examination.Results:The obtained data revealed that AZA-induced hepatic injury in the rats as evidenced by the signifi cant increase in serum aspartate aminotransferase,alanine aminotransferase,alkaline phosphatase,cholesterol,and direct bilirubin as well as hepatic malondialdehyde level accompanied with signifi cant decrease in reduced glutathione content and total antioxidant capacity in the liver.Moreover,body weight gain showed the signifi cant decrease and relative liver weight showed the signifi cant increase on AZA treatment.The sequential signifi cant changes in biochemical parameters were accompanied by severe histological changes in the liver tissue,including hepatocytes disorganization with pyknotic nuclei,fatty degeneration,congestion,fi brosis,and bile duct necrosis around the portal tract.The areas of hemorrhages in blood vessels and in between hepatocytes were also seen.However,the results showed the potential hepatoprotective effects of CFME and FSME against AZA-induced liver injury and oxidative stress.They succeeded in restoring the biochemical parameters and improving the histological picture of the liver.This improvement was more pronounced in the rats pretreated with FSME.Conclusion:It could be concluded that CFME and FSME have hepatoprotective potentials against AZA probably due to their antioxidant properties and radical scavenging activity.展开更多
Using computer-aided design three-dimensional simulation technology,the supply voltage scaled dependency of the recovery of single event upset and charge collection in static random-access memory cells are investigate...Using computer-aided design three-dimensional simulation technology,the supply voltage scaled dependency of the recovery of single event upset and charge collection in static random-access memory cells are investigated.It reveals that the recovery linear energy transfer threshold decreases with the supply voltage reducing,which is quite attractive for dynamic voltage scaling and subthreshold circuit radiation-hardened design.Additionally,the effect of supply voltage on charge collection is also investigated.It is concluded that the supply voltage mainly affects the bipolar gain of the parasitical bipolar junction transistor（BJT） and the existence of the source plays an important role in supply voltage variation.展开更多
The structure and the characteristic power spectrum of a new lower hybrid wave antenna on the HT-7 tokamak are briefly described in this paper. The main experimental properties with the new antenna have been demonstra...The structure and the characteristic power spectrum of a new lower hybrid wave antenna on the HT-7 tokamak are briefly described in this paper. The main experimental properties with the new antenna have been demonstrated by showing the dependence of current drive efficiency and particle confinement time on phase difference between adjacent waveguides of the antenna. A few preliminary analyses about the experimental results are also discussed in the paper.展开更多
Floating flower art belongs to a category of embossing art,which is a kind of garden art that makes plant materials into flower decorations after treatment.In this paper,the whole technological process of making float...Floating flower art belongs to a category of embossing art,which is a kind of garden art that makes plant materials into flower decorations after treatment.In this paper,the whole technological process of making floating flower products was described in detail,and the unique manufacturing process of Japanese embossing brand "Sola Cube" was analyzed,in order to provide some theoretical reference for floating flower lovers and promote its development in China.展开更多
基金supported by the Study on Resource Collection and New Variety Breeding of the Guizhou Mountainous Characteristic Flower C.goeringii(QianKeHe[2022]General 107)the Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Karst Mountainous Areas of Southwestern China,Guizhou Academy of Forestry.
文摘Cymbidium goeringii is an economically important ornamental plant,and flower color is one of the main features of C.goeringii that contributes to its high economic value.To clarify the molecular mechanisms underlying the role of anthocyanins in mediating differences in color among varieties,liquid chromatography–tandem mass spectrometry was used to perform anthocyanin-targeted metabolomics of seven C.goeringii varieties,including‘Jin Qian Yuan’(JQY),‘Jin Xiu Qian Yuan’(JXQY),‘Miao Jiang Su Die’(MJSD),‘Qian Ming Su’(QMS),‘Shi Chan’(SC),and‘Yang Ming Su’(YMS),as well as the C.goeringii.We detected 64 anthocyanins,including cyanidins,delphinidins,malvidins,pelargonidins,peonidins,petunidins,procyanidins,and flavonoids.We identified six shared differentially accumulated metabolites(DAMs),including cyanidin-3-O-rutinoside,delphinidin-3-Osophoroside,pelargonidin-3-O-rutinoside,peonidin-3-O-(6-O-malonyl-beta-D-glucoside),peonidin-3-Osophoroside,and chalcone.Most DAMs were enriched in the anthocyanin biosynthesis pathway.Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed that the differentially expressed metabolites were significantly enriched in the anthocyanin biosynthesis pathway.Analysis of the content of differentially expressed metabolites indicated that peonidin-3-O-(6-O-malonyl-beta-D-glucoside)was the key metabolite underlying color differences among C.goeringii varieties.Procyanidin B2,pelargonidin-3-O-galactoside,and naringenin might also affect the color formation of JQY and QMS,SC,and MJSD,respectively.The results of this study shed light on the metabolic mechanism underlying flower color differences in C.goeringii at the molecular level.Our findings will aid future studies of the mechanism of flower color regulation in C.goeringii and have implications for the breeding of new varieties.
基金supported by Jilin Province Science and Technology Development Program(20220202014NC)the National Natural Science Foundation of China(#31471565 and#31170259).
文摘DNA methylation is an important epigenetic regulatory mechanism,it regulates gene expression by recruiting proteins involved in gene repression or by inhibiting the binding of transcription factor(s)to DNA.In this study,a novel methyltransferase 2a gene(Zmet2a)was cloned in maize and identified by polymerase chain reaction-base(PCR-base)using a bioinformatics strategy.The Zmet2a cDNA sequence is 2739 bp long and translates to 912 amino acid peptides.The Zmet2a protein revealed that it contains BAH and CHROMO structural domains,is a non-transmembrane protein that is hydrophilically unstable,and has no signal peptide structure.Meanwhile,we verified the biological roles of Zmet2a using transgenic Arabidopsis overexpressing Zmet2a and Zmet2a-knockout maize.Transgenic Zmet2a Arabidopsis thaliana showed highly significant advancement inflowering time,and Zmet2a-knockout maize showed advancement inflowering time,with significant changes in several traits.Altogether,these report the role of Zmet2a in the regulation offlowering time,which will lay a foundation for revealing the biological function and epigenetic regulation mechanism of Zmet2a in the growth,development andflowering of maize.
文摘Aim:The present study was conducted to investigate the protective effect of chamomile fl owers methanolic extract(CFME)and fennel seeds methanolic extract(FSME)on azathioprine(AZA),an immunosuppressant drug,which induced a liver injury and oxidative stress in rats.Methods:Rats were divided into 6 groups(8 rats each)and treated orally for 28 consecutive days as follows.Group 1:rats were given normal saline and used as controls;group 2:rats treated with CFME(200 mg/kg);group 3:rats treated with FSME(200 mg/kg);group 4:rats treated with AZA(25 mg/kg);and groups 5 and 6:rats treated with CFME(200 mg/kg)or FSME(200 mg/kg)15 min prior to AZA(25 mg/kg)treatment.At the end of experimental period,blood and liver samples were collected from all groups for biochemical analysis and histological examination.Results:The obtained data revealed that AZA-induced hepatic injury in the rats as evidenced by the signifi cant increase in serum aspartate aminotransferase,alanine aminotransferase,alkaline phosphatase,cholesterol,and direct bilirubin as well as hepatic malondialdehyde level accompanied with signifi cant decrease in reduced glutathione content and total antioxidant capacity in the liver.Moreover,body weight gain showed the signifi cant decrease and relative liver weight showed the signifi cant increase on AZA treatment.The sequential signifi cant changes in biochemical parameters were accompanied by severe histological changes in the liver tissue,including hepatocytes disorganization with pyknotic nuclei,fatty degeneration,congestion,fi brosis,and bile duct necrosis around the portal tract.The areas of hemorrhages in blood vessels and in between hepatocytes were also seen.However,the results showed the potential hepatoprotective effects of CFME and FSME against AZA-induced liver injury and oxidative stress.They succeeded in restoring the biochemical parameters and improving the histological picture of the liver.This improvement was more pronounced in the rats pretreated with FSME.Conclusion:It could be concluded that CFME and FSME have hepatoprotective potentials against AZA probably due to their antioxidant properties and radical scavenging activity.
基金Project supported by the State Key Program of the National Natural Science Foundation of China (Grant No. 60836004)Hunan Provincial Innovation Foundation for Postgraduates,China (Grant No. CX2011B026)
文摘Using computer-aided design three-dimensional simulation technology,the supply voltage scaled dependency of the recovery of single event upset and charge collection in static random-access memory cells are investigated.It reveals that the recovery linear energy transfer threshold decreases with the supply voltage reducing,which is quite attractive for dynamic voltage scaling and subthreshold circuit radiation-hardened design.Additionally,the effect of supply voltage on charge collection is also investigated.It is concluded that the supply voltage mainly affects the bipolar gain of the parasitical bipolar junction transistor（BJT） and the existence of the source plays an important role in supply voltage variation.
基金The project supported by the National Natural Science Foundation of China (No. 10205015)
文摘The structure and the characteristic power spectrum of a new lower hybrid wave antenna on the HT-7 tokamak are briefly described in this paper. The main experimental properties with the new antenna have been demonstrated by showing the dependence of current drive efficiency and particle confinement time on phase difference between adjacent waveguides of the antenna. A few preliminary analyses about the experimental results are also discussed in the paper.
基金Sponsored by Provincial Innovation and Entrepreneurship Training Project for College Students in 2018(201812622013x)College Students’ Innovation and Entrepreneurship Training Project in 2017(CJDC2017045)
文摘Floating flower art belongs to a category of embossing art,which is a kind of garden art that makes plant materials into flower decorations after treatment.In this paper,the whole technological process of making floating flower products was described in detail,and the unique manufacturing process of Japanese embossing brand "Sola Cube" was analyzed,in order to provide some theoretical reference for floating flower lovers and promote its development in China.
