Terpenoids are a class of high value-added natural products with a variety of biological functions.Genetically engineered microorganisms,such as those of Escherichia coli and Saccharomyces cerevisiae,have merits in pr...Terpenoids are a class of high value-added natural products with a variety of biological functions.Genetically engineered microorganisms,such as those of Escherichia coli and Saccharomyces cerevisiae,have merits in producing plant or fungus-derived terpenoids,due to their mature genetic manipulation,simple nutrient demand and fast growth.Oxygen,as a key environmental factor,is particularly important to microbial metabolism and growth,and suitable oxygen supply is viewed as a prerequisite for realizing highly efficient production of terpenoids by engineered microorganisms.In this article,the role of oxygen in regulating terpenoid bioproduction is overviewed from the viewpoints of cellular carbon metabolism,energy metabolism and terpenoid anabolism.Strategies on adjusting oxygen availability to microorganisms,including genetic modification of cellular metabolism related with oxygen utilization,are summarized and discussed,to provide helpful information for further improvement of terpenoid biosynthesis by microbes.展开更多
In order to fulfill the needs of life-support oxygen supply during the development of plateau mineral resources,four oxygen supply patterns suitable for the plateau mine in specific environment were developed:tunnel f...In order to fulfill the needs of life-support oxygen supply during the development of plateau mineral resources,four oxygen supply patterns suitable for the plateau mine in specific environment were developed:tunnel face diffusive oxygen supply,tunnel oxygen-bar car,carried oxygen cylinder and portable oxygen generator. Through the study of safety oxygen supply experiments in low- pressure plateau areas,the mathematical relationship between maximum integration of secure oxygen volume and altitude has been achieved. Oxygen supply safety control should follow this relationship in plateau mines during the time of executing tunnel face or in room air diffusive oxygen supply. The application results of life-support oxygen supplement technologies in the development of mineral resources in high altitude areas show that the oxygen supply for tunneling miners in plateau mines can not only effectively enhance the oxygen saturation,reduce the pulse rate and the breath rate per minute,but also improve various symptoms caused by altitude hypoxia and high-intensity physical labor.展开更多
At high altitudes, an Aviation Oxygen Supply System （AOSS） protects pilots from low pressure and hypoxia by continuously providing oxygen corresponding to the pilots＇ dynamic respiratory properties. An AOSS mainly ...At high altitudes, an Aviation Oxygen Supply System （AOSS） protects pilots from low pressure and hypoxia by continuously providing oxygen corresponding to the pilots＇ dynamic respiratory properties. An AOSS mainly consists of oxygen supercharging machines which are used in a high-altitude flight cabin to supply pressurized oxygen to pilots. Therefore, it is of great significance to study the airflow dynamic characteristics of an AOSS for safe, continuous, and efficient oxygen supply. In this paper, an AOSS is firstly simplified and considered as a mechanical ventilation system. Then, its corresponding mathematical model is constructed. Next, to verify the mathematical model, a prototype AOSS with a lung simulator is proposed for an experimental study. Afterwards, to build a foundation for the optimization of the AOSS, the airflow dynamic characteristics of an aircraft are analyzed, and the effects of key parameters on the respiration system are researched. Through experimental and simulation studies, it can be concluded that the mathematical model is effective. Subsequently, for stability during the respiration process, we consider setting the equivalent throttling areas of the inspiration and expiration pipelines smaller within certain limits; additionally, an excessively high oxygen supply pressure will disturb smooth airflow, and in a low-pressure environment, the pressure can be 84 cmH20 lower than the standard atmospheric pressure. This research can be referred to in the design of an oxygen supply system and the study on optimization of airflow dynamic characteristics.展开更多
The hypoxic environment at high altitudes causes various sleep disorders.Diffuse oxygen enrichment is an effective way to alleviate sleep disorders and improve the built environment in high altitude areas.In this stud...The hypoxic environment at high altitudes causes various sleep disorders.Diffuse oxygen enrichment is an effective way to alleviate sleep disorders and improve the built environment in high altitude areas.In this study,a novel point source local diffuse oxygen supply method was proposed to improve the sleeping oxygen environment.The oxygen supply performance was investigated by the computational fluid dynamics(CFD)method including the oxygen concentration and air velocity distributions.A sleeping experiment was conducted on the plateau to validate the CFD model.The occupied zone including the inhalation zone and the active zone was defined.The results showed that the oxygen concentration showed a rapid rise,then decreased slowly,and finally tended to be stable.The oxygen concentration after stabilization was remarkably influenced by indoor ventilation rate.The sleeping environment’s improvement was examined considering the oxygen enrichment efficiency,uniformity,stability and human comfort demand.The optimal strategies were recommended with a ventilation rate of 1 air change per hour,supplied oxygen concentration of 90%;and jet distance of 0.50 m.The study contributes to improving the oxygen environment and human sleep quality in an effective and energy-saving approach to the sustainable development of buildings in high altitude areas.展开更多
Long chain dicarboxylic acid (DCA) produced by Candida tropicalis is produced in an aerobic viscous fermentation system. The gas-liquid transport resistance can be overcome and the oxygen supply can be increased by ad...Long chain dicarboxylic acid (DCA) produced by Candida tropicalis is produced in an aerobic viscous fermentation system. The gas-liquid transport resistance can be overcome and the oxygen supply can be increased by adding hydrogen peroxide (H_2O_2) to the fermentation system. This paper shows that H_2O_2 can not only enhance the oxygen supply but also change the metabolism by inducing cytochrome P450, the key enzyme of α, ω-oxidation. When C. tropicalis was cultivated in a 3-liter bioreactor using the combination of aeration and hydrogen peroxide feeding, the DCA yield increased about 10% except at the beginning of H_2O_2 feeding. The experiments showed that the maximum activities of P450 could be induced at 2 mmol·L -1 H_2O_2. By adding H_2O_2, the DCA yield in a 22-liter bioreactor was increased 25.3% to 153.9 g/L.展开更多
Bacterial biofilms,especially those caused by multidrug-resistant bacteria,have emerged as one of the greatest dangers to global public health.The acceleration of antimicrobial resistance to conventional an-tibiotics ...Bacterial biofilms,especially those caused by multidrug-resistant bacteria,have emerged as one of the greatest dangers to global public health.The acceleration of antimicrobial resistance to conventional an-tibiotics and the severe lack of new drugs necessitates the development of novel agents for biofilm eradication.Photodynamic therapy(PDT)is a promising non-antibiotic method for treating bacterial infections.However,its application in biofilm eradication is hampered by the hypoxic microenvironment of biofilms and the physical protection of extracellular polymeric substances.In this study,we develop a composite nanoplatform with oxygen(O_(2))self-supplying and heat-sensitizing capabilities to improve the PDT efficacy against biofilms.CaO_(2)/ICG@PDA nanoparticles(CIP NPs)are fabricated by combining calcium peroxide(CaO_(2))with the photosensitizer indocyanine green(ICG)via electrostatic interactions,followed by coating with polydopamine(PDA).The CIP NPs can gradually generate O_(2)in response to the acidic microenvironment of the biofilm,thereby alleviating its hypoxic state.Under near-infrared(NIR)irradiation,the nanoplatform converts O_(2)into a significant amount of singlet oxygen(^(1)O_(2))and heat to eradicate biofilm.The generated heat enhances the release of O_(2),accelerates the generation of^(1)O_(2)in PDT,increases cell membrane permeability,and increases bacterial sensitivity to^(1)O_(2).This nanoplatform significantly improves the efficacy of PDT in eradicating biofilm-dwelling bacteria without fostering drug resistance.Experiments on biofilm eradication demonstrate that this nanoplatform can eradicate over 99.9999%of methicillin-resistant Staphylococcus aureus(MRSA)biofilms under 5-min NIR irradiation.Notably,these integrated advantages enable the system to promote the healing of MRSA biofilm-infected wounds with negligible toxicity in vivo,indicating great promise for overcoming the obstacles associated with bacterial biofilm eradication.展开更多
Coalfield fires are considered a global crisis that contributes significantly to environmental destruction and loss of coal resources and poses a serious threat to human safety and health. In this paper, research rela...Coalfield fires are considered a global crisis that contributes significantly to environmental destruction and loss of coal resources and poses a serious threat to human safety and health. In this paper, research related to the initiation, development, and evolution of coalfield fires is reviewed. The existing detection and control techniques of coalfield fires are also reviewed. Traditional firefighting is associated with waste of resources, potential risks of recrudescence, potential safety hazards, extensive and expensive engineering works, and power shortages. Recently,coalfield fires have been recognized as having significant potential for energy conservation and heat energy recovery. Thermoelectric power generation is regarded as a suitable technology for the utilization of heat from coalfield fires. The extraction of heat from coalfield fires can also control coalfield fires and prevent reignition leading to combustion. Technologies for absorbing heat from burning coal and overlying rocks are also analyzed. In addition, the control mode of "three-region linkage" is proposed to improve firefighting efficiency. Integrating heat energy recovery with firefighting is an innovative method to control coalfield fires.展开更多
Objective:To establish a rat model of transfusion-related acute lung injury(TRALI)with coronary heart disease(CHD),and to analyze the safety of blood transfusion through oxygen balance.Methods:Forty-five 10-day-old ma...Objective:To establish a rat model of transfusion-related acute lung injury(TRALI)with coronary heart disease(CHD),and to analyze the safety of blood transfusion through oxygen balance.Methods:Forty-five 10-day-old male Wistar rats were purchased,and 35 of them were fed with high-fat diet to establish coronary heart disease rat models,and then 20 of them were selected to establish rat models of transfusion-related acute lung injury with coronary heart disease(model group,10 rats),positive acute lung injury group(positive group,5 rats)and negative acute lung injury group(negative group,5 rats),and the lung histomorphology,pathological score and wet/dry weight ratio were compared.Then,another 15 rats with coronary heart disease were selected and infused with mutant Hb,rHb1.1 and rHb2.0 with the same osmotic pressure through femoral vein catheterization,respectively,and were divided into mutant Hb group,rHb1.1 group and rHb2.0 group,with 5 rats in each group,and 5 healthy rats were combined as control group.The MAP,HR and blood gas values of mesenteric artery of rats were compared at 0,30,60 and 90 min after infusion.Results:(1)Rats in the model group and the positive group showed symptoms such as irregular and shallow breathing,increased oral and nasal secretions,and audible wheezing,which were consistent with the symptoms of acute lung injury.Comparison of lung histological score and lung tissue wet/dry ratio in three groups:There was no significant difference in lung histological score and lung tissue wet/dry ratio(P>0.05),but they were higher than those in the negative group(P<0.05).(2)During hemoglobin infusion,the MAP of mutant Hb group,rHb1.1 group and rHb2.0 group was higher than that of the control group,while the pH and PaCO2were lower than those of the control group(P<0.05),and there was no significant difference in QSMA(P>0.05).In the mutant Hb group,MAP returned to normal at 30-60 min after infusion(P>0.05),but MAP increased again at 90 min after infusion(P<0.05),and QSMA increased significantly at 60 min after transfusion(P<0.05).The pH value was lower than the normal value and the PaCO2was higher than the normal value within 90 min of infusion(P<0.05),and the HCO3-level returned to normal after 30 min of infusion(P>0.05).In rHb 1.1 group,MAP returned to normal,QSMA remained at normal level(P>0.05),and pH,PaCO2and HCO3-returned to normal after 60 min of infusion(P>0.05);In rHb 2.0 group,the levels of MAP,pH,PaCO2and HCO3-returned to normal after 30 min of infusion(P>0.05),and QSMA remained normal during infusion(P>0.05).Conclusion:The rat model of transfusion-relatted acute lung injury with coronary heart disease can be successfully established by injecting LPS into the rat model of coronary heart disease,and the infusion of recombinant hemoglobin can improve the balance of blood supply in rats,in which the infusion of rHb2.0 can better correct the metabolic acidosis.展开更多
The high efficient blowing technique includes increasing oxygen supply intensity and optimizing slag forming. The oxygen supply intensity on 300 t converters of No. 1 steelmaking shop at Baosteel reaches 3.83 m^3/（t ...The high efficient blowing technique includes increasing oxygen supply intensity and optimizing slag forming. The oxygen supply intensity on 300 t converters of No. 1 steelmaking shop at Baosteel reaches 3.83 m^3/（t · min）, and at Taiyuan Steel, Lianyuan Steel, Pingxiang Steel and other steel plants, the oxygen supply intensity on medium converters is in the range of 4.0--4.4m^3/（t · min）. The productivity of converter can be increased by 8% -- 15% with adopting this technique. The whole technique, including design and manufacture of lance nozzle with reasonable pacnolontenue of outlets, technique of oxygen supply and slag forming, has been developed by CISRI to meet the need of technique transfer.展开更多
基金This work was supported by the National Key R&D Program of China(Nos.2019YFA0904800 and 2018YFA0901900)the National Natural Science Foundation of China(No.31770037)。
文摘Terpenoids are a class of high value-added natural products with a variety of biological functions.Genetically engineered microorganisms,such as those of Escherichia coli and Saccharomyces cerevisiae,have merits in producing plant or fungus-derived terpenoids,due to their mature genetic manipulation,simple nutrient demand and fast growth.Oxygen,as a key environmental factor,is particularly important to microbial metabolism and growth,and suitable oxygen supply is viewed as a prerequisite for realizing highly efficient production of terpenoids by engineered microorganisms.In this article,the role of oxygen in regulating terpenoid bioproduction is overviewed from the viewpoints of cellular carbon metabolism,energy metabolism and terpenoid anabolism.Strategies on adjusting oxygen availability to microorganisms,including genetic modification of cellular metabolism related with oxygen utilization,are summarized and discussed,to provide helpful information for further improvement of terpenoid biosynthesis by microbes.
基金"973"National Key Basic Research and Development Program(No.2012CB518202)Project of Qinghai Development of Science and Technology(No.2011-N-150)
文摘In order to fulfill the needs of life-support oxygen supply during the development of plateau mineral resources,four oxygen supply patterns suitable for the plateau mine in specific environment were developed:tunnel face diffusive oxygen supply,tunnel oxygen-bar car,carried oxygen cylinder and portable oxygen generator. Through the study of safety oxygen supply experiments in low- pressure plateau areas,the mathematical relationship between maximum integration of secure oxygen volume and altitude has been achieved. Oxygen supply safety control should follow this relationship in plateau mines during the time of executing tunnel face or in room air diffusive oxygen supply. The application results of life-support oxygen supplement technologies in the development of mineral resources in high altitude areas show that the oxygen supply for tunneling miners in plateau mines can not only effectively enhance the oxygen saturation,reduce the pulse rate and the breath rate per minute,but also improve various symptoms caused by altitude hypoxia and high-intensity physical labor.
文摘At high altitudes, an Aviation Oxygen Supply System （AOSS） protects pilots from low pressure and hypoxia by continuously providing oxygen corresponding to the pilots＇ dynamic respiratory properties. An AOSS mainly consists of oxygen supercharging machines which are used in a high-altitude flight cabin to supply pressurized oxygen to pilots. Therefore, it is of great significance to study the airflow dynamic characteristics of an AOSS for safe, continuous, and efficient oxygen supply. In this paper, an AOSS is firstly simplified and considered as a mechanical ventilation system. Then, its corresponding mathematical model is constructed. Next, to verify the mathematical model, a prototype AOSS with a lung simulator is proposed for an experimental study. Afterwards, to build a foundation for the optimization of the AOSS, the airflow dynamic characteristics of an aircraft are analyzed, and the effects of key parameters on the respiration system are researched. Through experimental and simulation studies, it can be concluded that the mathematical model is effective. Subsequently, for stability during the respiration process, we consider setting the equivalent throttling areas of the inspiration and expiration pipelines smaller within certain limits; additionally, an excessively high oxygen supply pressure will disturb smooth airflow, and in a low-pressure environment, the pressure can be 84 cmH20 lower than the standard atmospheric pressure. This research can be referred to in the design of an oxygen supply system and the study on optimization of airflow dynamic characteristics.
基金We extend our gratitude to the funding supports of the National Natural Science Foundation of China(No.51878532)the Shaanxi Province Natural Science Foundation Research Project of China(No.2019JQ-392)the Independent Research and Development project of State Key Laboratory of Green Building in Western China(No.LSZZ202008).
文摘The hypoxic environment at high altitudes causes various sleep disorders.Diffuse oxygen enrichment is an effective way to alleviate sleep disorders and improve the built environment in high altitude areas.In this study,a novel point source local diffuse oxygen supply method was proposed to improve the sleeping oxygen environment.The oxygen supply performance was investigated by the computational fluid dynamics(CFD)method including the oxygen concentration and air velocity distributions.A sleeping experiment was conducted on the plateau to validate the CFD model.The occupied zone including the inhalation zone and the active zone was defined.The results showed that the oxygen concentration showed a rapid rise,then decreased slowly,and finally tended to be stable.The oxygen concentration after stabilization was remarkably influenced by indoor ventilation rate.The sleeping environment’s improvement was examined considering the oxygen enrichment efficiency,uniformity,stability and human comfort demand.The optimal strategies were recommended with a ventilation rate of 1 air change per hour,supplied oxygen concentration of 90%;and jet distance of 0.50 m.The study contributes to improving the oxygen environment and human sleep quality in an effective and energy-saving approach to the sustainable development of buildings in high altitude areas.
基金Supported by the National Natural Science Foundationof China (Nos.3 0 0 0 0 0 0 3 and 2 99760 2 2 )
文摘Long chain dicarboxylic acid (DCA) produced by Candida tropicalis is produced in an aerobic viscous fermentation system. The gas-liquid transport resistance can be overcome and the oxygen supply can be increased by adding hydrogen peroxide (H_2O_2) to the fermentation system. This paper shows that H_2O_2 can not only enhance the oxygen supply but also change the metabolism by inducing cytochrome P450, the key enzyme of α, ω-oxidation. When C. tropicalis was cultivated in a 3-liter bioreactor using the combination of aeration and hydrogen peroxide feeding, the DCA yield increased about 10% except at the beginning of H_2O_2 feeding. The experiments showed that the maximum activities of P450 could be induced at 2 mmol·L -1 H_2O_2. By adding H_2O_2, the DCA yield in a 22-liter bioreactor was increased 25.3% to 153.9 g/L.
基金supported by the National Natural Science Foundation of China(No.22175125)the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(No.21KJA150008)the Key Laboratory of Polymeric Materials De-sign and Synthesis for Biomedical Function,Soochow University,and the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).
文摘Bacterial biofilms,especially those caused by multidrug-resistant bacteria,have emerged as one of the greatest dangers to global public health.The acceleration of antimicrobial resistance to conventional an-tibiotics and the severe lack of new drugs necessitates the development of novel agents for biofilm eradication.Photodynamic therapy(PDT)is a promising non-antibiotic method for treating bacterial infections.However,its application in biofilm eradication is hampered by the hypoxic microenvironment of biofilms and the physical protection of extracellular polymeric substances.In this study,we develop a composite nanoplatform with oxygen(O_(2))self-supplying and heat-sensitizing capabilities to improve the PDT efficacy against biofilms.CaO_(2)/ICG@PDA nanoparticles(CIP NPs)are fabricated by combining calcium peroxide(CaO_(2))with the photosensitizer indocyanine green(ICG)via electrostatic interactions,followed by coating with polydopamine(PDA).The CIP NPs can gradually generate O_(2)in response to the acidic microenvironment of the biofilm,thereby alleviating its hypoxic state.Under near-infrared(NIR)irradiation,the nanoplatform converts O_(2)into a significant amount of singlet oxygen(^(1)O_(2))and heat to eradicate biofilm.The generated heat enhances the release of O_(2),accelerates the generation of^(1)O_(2)in PDT,increases cell membrane permeability,and increases bacterial sensitivity to^(1)O_(2).This nanoplatform significantly improves the efficacy of PDT in eradicating biofilm-dwelling bacteria without fostering drug resistance.Experiments on biofilm eradication demonstrate that this nanoplatform can eradicate over 99.9999%of methicillin-resistant Staphylococcus aureus(MRSA)biofilms under 5-min NIR irradiation.Notably,these integrated advantages enable the system to promote the healing of MRSA biofilm-infected wounds with negligible toxicity in vivo,indicating great promise for overcoming the obstacles associated with bacterial biofilm eradication.
基金financially supported by the Fundamental Research Funds for the Central Universities of China(Nos.2017CXNL02 and 2652018098)the National Key Research and Development Program of China(No.2018YFC0808100)+1 种基金the 111 Project(No.B17041)the Natural Science Foundation of Jiangsu Province(No.BK20170277)
文摘Coalfield fires are considered a global crisis that contributes significantly to environmental destruction and loss of coal resources and poses a serious threat to human safety and health. In this paper, research related to the initiation, development, and evolution of coalfield fires is reviewed. The existing detection and control techniques of coalfield fires are also reviewed. Traditional firefighting is associated with waste of resources, potential risks of recrudescence, potential safety hazards, extensive and expensive engineering works, and power shortages. Recently,coalfield fires have been recognized as having significant potential for energy conservation and heat energy recovery. Thermoelectric power generation is regarded as a suitable technology for the utilization of heat from coalfield fires. The extraction of heat from coalfield fires can also control coalfield fires and prevent reignition leading to combustion. Technologies for absorbing heat from burning coal and overlying rocks are also analyzed. In addition, the control mode of "three-region linkage" is proposed to improve firefighting efficiency. Integrating heat energy recovery with firefighting is an innovative method to control coalfield fires.
文摘Objective:To establish a rat model of transfusion-related acute lung injury(TRALI)with coronary heart disease(CHD),and to analyze the safety of blood transfusion through oxygen balance.Methods:Forty-five 10-day-old male Wistar rats were purchased,and 35 of them were fed with high-fat diet to establish coronary heart disease rat models,and then 20 of them were selected to establish rat models of transfusion-related acute lung injury with coronary heart disease(model group,10 rats),positive acute lung injury group(positive group,5 rats)and negative acute lung injury group(negative group,5 rats),and the lung histomorphology,pathological score and wet/dry weight ratio were compared.Then,another 15 rats with coronary heart disease were selected and infused with mutant Hb,rHb1.1 and rHb2.0 with the same osmotic pressure through femoral vein catheterization,respectively,and were divided into mutant Hb group,rHb1.1 group and rHb2.0 group,with 5 rats in each group,and 5 healthy rats were combined as control group.The MAP,HR and blood gas values of mesenteric artery of rats were compared at 0,30,60 and 90 min after infusion.Results:(1)Rats in the model group and the positive group showed symptoms such as irregular and shallow breathing,increased oral and nasal secretions,and audible wheezing,which were consistent with the symptoms of acute lung injury.Comparison of lung histological score and lung tissue wet/dry ratio in three groups:There was no significant difference in lung histological score and lung tissue wet/dry ratio(P>0.05),but they were higher than those in the negative group(P<0.05).(2)During hemoglobin infusion,the MAP of mutant Hb group,rHb1.1 group and rHb2.0 group was higher than that of the control group,while the pH and PaCO2were lower than those of the control group(P<0.05),and there was no significant difference in QSMA(P>0.05).In the mutant Hb group,MAP returned to normal at 30-60 min after infusion(P>0.05),but MAP increased again at 90 min after infusion(P<0.05),and QSMA increased significantly at 60 min after transfusion(P<0.05).The pH value was lower than the normal value and the PaCO2was higher than the normal value within 90 min of infusion(P<0.05),and the HCO3-level returned to normal after 30 min of infusion(P>0.05).In rHb 1.1 group,MAP returned to normal,QSMA remained at normal level(P>0.05),and pH,PaCO2and HCO3-returned to normal after 60 min of infusion(P>0.05);In rHb 2.0 group,the levels of MAP,pH,PaCO2and HCO3-returned to normal after 30 min of infusion(P>0.05),and QSMA remained normal during infusion(P>0.05).Conclusion:The rat model of transfusion-relatted acute lung injury with coronary heart disease can be successfully established by injecting LPS into the rat model of coronary heart disease,and the infusion of recombinant hemoglobin can improve the balance of blood supply in rats,in which the infusion of rHb2.0 can better correct the metabolic acidosis.
文摘The high efficient blowing technique includes increasing oxygen supply intensity and optimizing slag forming. The oxygen supply intensity on 300 t converters of No. 1 steelmaking shop at Baosteel reaches 3.83 m^3/（t · min）, and at Taiyuan Steel, Lianyuan Steel, Pingxiang Steel and other steel plants, the oxygen supply intensity on medium converters is in the range of 4.0--4.4m^3/（t · min）. The productivity of converter can be increased by 8% -- 15% with adopting this technique. The whole technique, including design and manufacture of lance nozzle with reasonable pacnolontenue of outlets, technique of oxygen supply and slag forming, has been developed by CISRI to meet the need of technique transfer.
