Anti-lipid peroxidation activity, reducing power and chelating power of flavonoid extracts from Oxytropis glabra DC, as well as their elimination effect on DPPH, superoxide anion and hydroxyl radicals were determined ...Anti-lipid peroxidation activity, reducing power and chelating power of flavonoid extracts from Oxytropis glabra DC, as well as their elimination effect on DPPH, superoxide anion and hydroxyl radicals were determined in the paper, so as to evaluate in vitro antioxidative activities of flavonoid extracted from O. glabra. The results showed that flavonoid from O. glabra had fairly strong anti-lipid peroxidation. The antioxidation effects on rapeseed oil successively were vitamin C 〉 sample 〉 vitamin E 〉 BHT 〉 CK; the effects on cottonseed oil successively were sample 〉 vitamin C 〉 BHT 〉 vitamin E 〉 CK; the effects on sunflower oil successively were sample 〉 vitamin C 〉 vitamin E 〉 BHT 〉 CK; the effects on lard successively were BHT 〉 vitamin C 〉 sample 〉 vitamin E 〉 CK. The samples had stronger clearance rate against DPPH, superoxide anion and hydroxyl radicals than control; the reducing power of sample was weaker than Vitamin C and stronger than Vitamin E ; chelating power of sample was weaker than EDTA. Flavonoid from O. glabra had the value for further development and utilization as natural antioxidant.展开更多
This study was conducted to optimize immunization program to gain the serum against swainsonine （SW） with high titer and to analyze the effects of polychinal antibody in treatment of loco disease of rabbits. Eightee...This study was conducted to optimize immunization program to gain the serum against swainsonine （SW） with high titer and to analyze the effects of polychinal antibody in treatment of loco disease of rabbits. Eighteen rabbits were randomly separated into control group, poisoning group and curing group, with six rabbits in each group. Rabbits in poisoning group and curing group were fed 10 g/（kg ~ d） powder of Oxytropis glabra DC every morning for 70 d till the rabbits in poisoning group began to die. From the 21＇t to 24＇h d of O. glabra administration, each of the rabbits in curing group was injected with 1 mL of polyclonal anti- body against SW every day. Blood samples were collected before O. glabra challenge, and then once every 7 d for the serological and immunological evaluation of serum. The resuhs showed that the serum alkaline phosphatase （AKP） activity and SW content in poisoning groups were significantly higher than those in control group on the 7th d （P 〈0.05 ） ; the serum aspartate transaminase （AST） and lactate dehydrogenase （LDH） activity in poisoning groups were significantly higher than those in control group on the 14th d （P 〈 0.05 ）, while the serum ct - mannosidase （AMA） and rate of E-rosette formation in poisoning groups were signifi- cantly lower than those in control group （ P 〈 0.05 ） ; the serum blood urea nitrogen （BUN）, creatinine （ CRE ） and glucose （ GLU ） concentration in poisoning groups were significantly higher than those in control group on the 21＇t d （P 〈 0.05 ）. By injecting serum against swainsonine, the serum AKP, LDH, AST, alanine transaminase （ALT） activity and contents of BUN, CRE, GLU, SW were reduced and serum AMA and rate of E-rosette formation were increased in curing group, by comparing with those in poisoning group. These results in this experiment indicated that polyclonal antibody against SW had good value of preventing from O. glabra DC.展开更多
基金Supported by"12th Five-Year Plan" National Science and Technology Support Program (2011BAD17B05)Doctoral Special Fund of Xinjiang Production and Construction Corps (2008JC07)Open Project for Key Laboratory of Tarim Animal Husbandry Science and Technology of Xinjiang Production Construction Corps (HS201207)
文摘Anti-lipid peroxidation activity, reducing power and chelating power of flavonoid extracts from Oxytropis glabra DC, as well as their elimination effect on DPPH, superoxide anion and hydroxyl radicals were determined in the paper, so as to evaluate in vitro antioxidative activities of flavonoid extracted from O. glabra. The results showed that flavonoid from O. glabra had fairly strong anti-lipid peroxidation. The antioxidation effects on rapeseed oil successively were vitamin C 〉 sample 〉 vitamin E 〉 BHT 〉 CK; the effects on cottonseed oil successively were sample 〉 vitamin C 〉 BHT 〉 vitamin E 〉 CK; the effects on sunflower oil successively were sample 〉 vitamin C 〉 vitamin E 〉 BHT 〉 CK; the effects on lard successively were BHT 〉 vitamin C 〉 sample 〉 vitamin E 〉 CK. The samples had stronger clearance rate against DPPH, superoxide anion and hydroxyl radicals than control; the reducing power of sample was weaker than Vitamin C and stronger than Vitamin E ; chelating power of sample was weaker than EDTA. Flavonoid from O. glabra had the value for further development and utilization as natural antioxidant.
基金Supported by Doctoral Fund of Xinjiang Production and Construction Corps(2008JC07)Open Fund of Key Laboratory of Tarim Animal Husbandry Science and Technology,Xin Jiang Production & Construction Corps(HS201207)
文摘This study was conducted to optimize immunization program to gain the serum against swainsonine （SW） with high titer and to analyze the effects of polychinal antibody in treatment of loco disease of rabbits. Eighteen rabbits were randomly separated into control group, poisoning group and curing group, with six rabbits in each group. Rabbits in poisoning group and curing group were fed 10 g/（kg ~ d） powder of Oxytropis glabra DC every morning for 70 d till the rabbits in poisoning group began to die. From the 21＇t to 24＇h d of O. glabra administration, each of the rabbits in curing group was injected with 1 mL of polyclonal anti- body against SW every day. Blood samples were collected before O. glabra challenge, and then once every 7 d for the serological and immunological evaluation of serum. The resuhs showed that the serum alkaline phosphatase （AKP） activity and SW content in poisoning groups were significantly higher than those in control group on the 7th d （P 〈0.05 ） ; the serum aspartate transaminase （AST） and lactate dehydrogenase （LDH） activity in poisoning groups were significantly higher than those in control group on the 14th d （P 〈 0.05 ）, while the serum ct - mannosidase （AMA） and rate of E-rosette formation in poisoning groups were signifi- cantly lower than those in control group （ P 〈 0.05 ） ; the serum blood urea nitrogen （BUN）, creatinine （ CRE ） and glucose （ GLU ） concentration in poisoning groups were significantly higher than those in control group on the 21＇t d （P 〈 0.05 ）. By injecting serum against swainsonine, the serum AKP, LDH, AST, alanine transaminase （ALT） activity and contents of BUN, CRE, GLU, SW were reduced and serum AMA and rate of E-rosette formation were increased in curing group, by comparing with those in poisoning group. These results in this experiment indicated that polyclonal antibody against SW had good value of preventing from O. glabra DC.
