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Local Perception on the Exploitation, the Current State and Taboos Related to Pangolins (Pholidota, Mammalia) by the Communities Living in the Tayna Nature Reserve and Its Surroundings (RNT) North Kivu DRC
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作者 David Kambale Malimbo Jonas Kambale Nyumu +6 位作者 Kasereka Vitekere Mbusa Mapoli Benzeth Visando Juakaly Mbumba Guy Crispin Tungaluna Francis Tarla Ngoy Steve 《Journal of Geoscience and Environment Protection》 2020年第12期18-35,共18页
Pangolins are currently considered the most endangered mammal species due to their high rank in local and international traffic. This species is protected in DRC and worldwide, unfortunately it is threatened by numero... Pangolins are currently considered the most endangered mammal species due to their high rank in local and international traffic. This species is protected in DRC and worldwide, unfortunately it is threatened by numerous human actions. The objective of this study is to identify the different reasons for which these animals are exploited, to provide information to determine from the opinions of the respondents the current state of the population from 2015 to today and to identify their hunting techniques by the local populations. We carried out surveys in the households of hunters, farmers and people practicing other professions in 12 villages, located within the RNT and surroundings areas. Two interview techniques were used: the individual interview and the participatory diagnostic technique. 175 people were interviewed individually. Nearly 97% of our respondents are motivated to consume pangolin as bush meat obtaining by hunting. The main reason for eating pangolin meat is 44% preference and 33% habit. The zone does not know the foreign solicitation of the exploitation of pangolins and its derivatives. The opinions of the population regarding the current state of pangolin in the Tayna Nature Reserve indicate that from 2015 to the present day the common pangolin “<em>Manis tricuspis</em>” has been abundant. Hunters use different hunting techniques, dominated by pickup for the <em>Manis tricuspis</em>, digging burrow, fire at the entrance of the burrow for <em>Manis gigantea</em> and the shotgun for the <em>Manis tetradactyla</em>. 展开更多
关键词 Local Perception EXPLOITATION Current State Taboos pangolins Communities Tayna Nature Reserve North Kivu/DRC
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The high diversity of SARS-CoV-2-related coronaviruses in pangolins alerts potential ecological risks 被引量:3
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作者 Min-Sheng Peng Jian-Bo Li +17 位作者 Zheng-Fei Cai Hang Liu Xiaolu Tang Ruochen Ying Jia-Nan Zhang Jia-Jun Tao Ting-Ting Yin Tao Zhang Jing-Yang Hu Ru-Nian Wu Zhong-Yin Zhou Zhi-Gang Zhang Li Yu Yong-Gang Yao Zheng-Li Shi Xue-Mei Lu Jian Lu Ya-Ping Zhang 《Zoological Research》 SCIE CAS CSCD 2021年第6期834-844,共11页
Understanding the zoonotic origin and evolution history of SARS-CoV-2 will provide critical insights for alerting and preventing future outbreaks.A significant gap remains for the possible role of pangolins as a reser... Understanding the zoonotic origin and evolution history of SARS-CoV-2 will provide critical insights for alerting and preventing future outbreaks.A significant gap remains for the possible role of pangolins as a reservoir of SARS-CoV-2 related coronaviruses(SC2r-CoVs).Here,we screened SC2r-CoVs in 172 samples from 163 pangolin individuals of four species,and detected positive signals in muscles of four Manis javanica and,for the first time,one M.pentadactyla.Phylogeographic analysis of pangolin mitochondrial DNA traced their origins from Southeast Asia.Using in-solution hybridization capture sequencing,we assembled a partial pangolin SC2r-CoV(pangolin-CoV)genome sequence of 22895 bp(MP20)from the M.pentadactyla sample.Phylogenetic analyses revealed MP20 was very closely related to pangolin-CoVs that were identified in M.javanica seized by Guangxi Customs.A genetic contribution of bat coronavirus to pangolin-CoVs via recombination was indicated.Our analysis revealed that the genetic diversity of pangolin-CoVs is substantially higher than previously anticipated.Given the potential infectivity of pangolin-CoVs,the high genetic diversity of pangolin-CoVs alerts the ecological risk of zoonotic evolution and transmission of pathogenic SC2r-CoVs. 展开更多
关键词 SARS-CoV-2 PANGOLIN Recombination DIVERSITY SEQUENCING MTDNA
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Virome in healthy pangolins reveals compatibility with multiple potentially zoonotic viruses
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作者 Feng-Juan Tian Jing Li +9 位作者 Wen-Li Liu Yu-Jie Liu Yun-Jia Hu Qi-Hang Tu Yang Li Yu Bai Mang Shi Teng-Cheng Que Yan-Ling Hu Yi-Gang Tong 《Zoological Research》 SCIE CAS CSCD 2022年第6期977-988,共12页
Previous studies have identified multiple viruses in dead or severely diseased pangolins,but descriptions of the virome in healthy pangolins are lacking.This poses a greater risk of cross-species transmission due to p... Previous studies have identified multiple viruses in dead or severely diseased pangolins,but descriptions of the virome in healthy pangolins are lacking.This poses a greater risk of cross-species transmission due to poor preventive awareness and frequent interactions with breeders.In this study,we investigated the viral composition of 34 pangolins with no signs of disease at the time of sampling and characterized a large number of arthropodassociated viruses belonging to 11 families and vertebrate viruses belonging to eight families,including those with pathogenic potential in humans and animals.Several important vertebrate viruses were identified in the pangolins,including parvovirus,pestivirus,and picobirnavirus.The picobirnavirus was clustered with human and grey teal picobirnaviruses.Viruses with cross-species transmission ability were also identified,including circovirus,rotavirus,and astrovirus.Our study revealed that pangolins are frequently exposed to arthropod-associated viruses in the wild and can carry many vertebrate viruses under natural conditions.This study provides important insights into the virome of pangolins,underscoring the importance of monitoring potential pathogens in healthy pangolins to prevent outbreaks of infectious diseases in domesticated animals and humans. 展开更多
关键词 PANGOLIN Virome Arthropod-associated viruses Cross-species transmission ZOONOSES
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Exploitation of Pangolins (Pholidota, Mammalia) by Communities Living in and around the Tayna Nature Reserve (RNT) North Kivu, Democratic Republic of Congo (DRC)
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作者 David Kambale Malimbo Jonas Kambale Nyumu +6 位作者 Kasereka Vitekere Joel Mapoli Benzeth Visando Juakaly Mbumba Guy Crispin Tungaluna Francis Tarla Yan Hua 《Journal of Geoscience and Environment Protection》 2020年第4期1-17,共17页
Pangolin exploitation has recently increased in diverse forms such as bushmeat consumption;species or parts trade, medical use. This species is protected in Democratic Republic of Congo (DRC) and worldwide, unfortunat... Pangolin exploitation has recently increased in diverse forms such as bushmeat consumption;species or parts trade, medical use. This species is protected in Democratic Republic of Congo (DRC) and worldwide, unfortunately it is threatened by numerous human actions. The purpose of this study was to determine the profiles of actors using pangolins, the reasons for pangolin exploitation and its products and their use within the local community in and surrounding the reserve. We carried out surveys in the households of hunters, farmers and people practicing other professions in 12 villages, located within the Tayna Nature Reserve (RNT) and surroundings areas. Two interview techniques were used: the individual interview and the participatory diagnostic technique. During one month of data collection, 175 respondents were interviewed. Respondents had diverse profiles according to sex, age, education level and ethnicity. Men were more represented as actors in pangolin exploitation (71.70%) than women (28.30%);the most represented age interval was 20 - 30 years. The education level is distinct amongst respondents: illiterate (37%), primary school (32.4%) and graduate from university (2.9%). An average number of respondents are motivated to consume pangolin as meat obtained by hunting at 50%. The main reason for its consumption is preference (44%) and habit (33%). The scales are pangolin derivatives most recognized by local people and widely used in the pharmacopoeia. Consequently, pangolin protection needs some rigorous measures to reinforce the current law implementation. 展开更多
关键词 PANGOLIN Derivative Products Local Community Exploitation Traditional Medicine and Tayna Nature Reserve
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Evolution and conservation genetics of pangolins 被引量:1
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作者 Tongtong GU Jingyang HU Li YU 《Integrative Zoology》 SCIE CSCD 2024年第3期426-441,共16页
Pangolins(Pholidota,Manidae)are classified as an evolutionarily distinct and globally endangered mammal due to their unique morphology(nail-like scales and a myrmecophagous diet)and being the victim of heavy poaching ... Pangolins(Pholidota,Manidae)are classified as an evolutionarily distinct and globally endangered mammal due to their unique morphology(nail-like scales and a myrmecophagous diet)and being the victim of heavy poaching and worldwide trafficking.As such,pangolins serve as a textbook example for studying the special phenotypic evolutionary adaptations and conservation genetics of an endangered species.Recent years have demonstrated significant advancements in the fields of molecular genetics and genomics,which have translated to a series of important research achievements and breakthroughs concerning the evolution and conservation genetics of pangolins.This review comprehensively presents the hitherto advances in phylogeny,adaptive evolution,conservation genetics,and conservation genomics that are related to pangolins,which will provide an ample understanding of their diversity,molecular adaptation mechanisms,and evolutionary potentials.In addition,we highlight the priority of investigating species/population diversity among pangolins and suggest several avenues of research that are highly relevant for future pangolin conservation. 展开更多
关键词 adaptive evolution conservation genetics conservation genomics pangolins PHYLOGENY
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Comparative study of gut microbiota from captive and confiscatedrescued wild pangolins 被引量:3
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作者 Chunbing Liu Jingyang Hu +4 位作者 Yajiang Wu David M.Irwin Wu Chen Zhigang Zhang Li Yu 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2021年第9期825-835,共11页
Pangolins are among the most critically endangered animals due to widespread poaching and worldwide trafficking. Captive breeding is considered to be one way to protect them and increase the sizes of their populations... Pangolins are among the most critically endangered animals due to widespread poaching and worldwide trafficking. Captive breeding is considered to be one way to protect them and increase the sizes of their populations. However, comparative studies of captive and wild pangolins in the context of gut microbiota are rare. Here, the gut microbiome of captive and confiscated-rescued wild pangolins is compared, and the effects of different periods of captivity and captivity with and without antibiotic treatment are considered.We show that different diets and periods of captivity, as well as the application of antibiotic therapy, can alter gut community composition and abundance in pangolins. Compared to wild pangolins, captive pangolins have an increased capacity for chitin and cellulose/hemicellulose degradation, fatty acid metabolism, and short-chain fatty acid synthesis, but a reduced ability to metabolize exogenous substances. In addition to increasing the ability of the gut microbiota to metabolize nutrients in captivity, captive breeding imposes some risks for survival by resulting in a greater abundance of antibiotic resistance genes and virulence factors in captive pangolins than in wild pangolins. Our study is important for the development of guidelines for pangolin conservation, including health assessment, disease prevention, and rehabilitation of wild pangolin populations. 展开更多
关键词 PANGOLIN Gut microbiome CAPTIVITY Antibiotic resistance genes Virulence factors
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家蚕Wnt信号通路下游关键基因Pangolin转录剪接体X3的克隆和分析 被引量:1
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作者 王叶菁 付秋杰 +1 位作者 殷子晴 何华伟 《昆虫学报》 CAS CSCD 北大核心 2022年第12期1592-1597,共6页
【目的】克隆家蚕Bombyx mori Wnt信号通路下游关键基因Pangolin isoforms A/H/I/S转录剪接体X3(Pangolin X3),分析其序列和表达特征。【方法】从NCBI数据库检索家蚕Pangolin X3,根据其编码序列(coding sequence,CDS)设计引物,利用PCR... 【目的】克隆家蚕Bombyx mori Wnt信号通路下游关键基因Pangolin isoforms A/H/I/S转录剪接体X3(Pangolin X3),分析其序列和表达特征。【方法】从NCBI数据库检索家蚕Pangolin X3,根据其编码序列(coding sequence,CDS)设计引物,利用PCR从家蚕幼虫中肠和血淋巴中进行克隆并测序验证。利用SilkDB 3.0,SMART,多序列比对和系统发育树分析Pangolin X3的序列特征。利用qRT-PCR分析Pangolin X3在家蚕5龄第3天幼虫不同组织(头、血淋巴、体壁、性腺、中肠、前部丝腺、中部丝腺、后部丝腺、脂肪体和马氏管)中的相对表达水平。【结果】从家蚕幼虫中肠和血淋巴克隆了Pangolin X3(GenBank登录号:XM_038020921)的CDS,其开放阅读框长1560 bp,编码519个氨基酸残基,预测分子量为55.86 kD,预测等电点为7.53。Pangolin X3蛋白含有保守的β-catenin结合位点和HMG结构域,其氨基酸序列在不同的昆虫中比较保守,特别是与DNA结合的HMG结构域,而与β-catenin结合的N末端CTNNB1结构域部分氨基酸残基发生变异。组织表达谱显示,Pangolin X3在家蚕5龄第3天幼虫中肠、血淋巴和性腺中的相对表达水平较高,在头、体壁、前部丝腺、中部丝腺、后部丝腺、脂肪体和马氏管中的相对表达水平较低。【结论】本研究克隆了家蚕Pangolin X3,分析了其序列和表达特征,为深入研究家蚕Pangolin的生物学功能提供了基础。 展开更多
关键词 家蚕 WNT Pangolin X3 基因克隆 序列分析 表达特征
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The First Birth and Survival of Cub in Captivity of Critically Endangered Malayan Pangolin(Manis javanica) 被引量:3
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作者 余经裕 姜福林 +2 位作者 彭建军 殷西林 马晓华 《Agricultural Science & Technology》 CAS 2015年第10期2322-2323,2330,共3页
Two adult captive Malayan pangolin (Manis javanica) successfully mated on February 3, 2015, and gave birth to one healthy cub on April 8 in Pangolin's Breeding Center of College of Life Sciences, Chongqing Normal U... Two adult captive Malayan pangolin (Manis javanica) successfully mated on February 3, 2015, and gave birth to one healthy cub on April 8 in Pangolin's Breeding Center of College of Life Sciences, Chongqing Normal University. Current- ly, the two adults and the cub are in good state of health, and especially the cub is healthily growing according to its weight and other health indicators. This is the first birth and survival of cub of Malayan pangolins in captivity. It proves that Malayan pangolin can successfully breed in appropriate captivity conditions and the reproductive parameters of Malayan pangolin are also determined, which provide valuable reference for other species of pangolins in artificial breeding, and also give effective way for population growth, ecological restoration and species conservation for pangolin in the wild. 展开更多
关键词 Malayan pangolin (Manis javanica) BIRTH CAPTIVITY BREEDING
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Two-Body Abrasive Wear of the Surfaces of Pangolin Scales 被引量:13
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作者 Jin Tong Tie-biao Lu +3 位作者 Yun-hai Ma Heng-kun Wang Lu-quan Ren R. D. Arnell 《Journal of Bionic Engineering》 SCIE EI CSCD 2007年第2期77-84,共8页
The Pangolin, a soil-burrowing animal, is covered with scales. These scales are often abraded by soil and rock and their surface is corrugated. The abrasive wear of the surface of the scales was examined. The scales w... The Pangolin, a soil-burrowing animal, is covered with scales. These scales are often abraded by soil and rock and their surface is corrugated. The abrasive wear of the surface of the scales was examined. The scales were taken from a pangolin that had died of natural causes. The tests were run on a rotary disc abrasive wear tester. The abrasive material was quartz sand (96.5 wt.%) and bentonite (3.5 wt.%). The morphology of the abraded surfaces and the abrasion were examined by stereoscopic microscopy and scanning electron microscopy. The concepts are proposed of "Guiding-Effect" and "Rolling-Effect" on the textured surfaces under free abrasive wear conditions and the critical dimensions of the "Rolling-Effect" are discussed. 展开更多
关键词 pangolin scale surface morphology abrasive wear
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A Review of the Engineering Role of Burrowing Animals: Implication of Chinese Pangolin as an Ecosystem Engineer 被引量:2
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作者 Song Sun Hongliang Dou +9 位作者 Shichao Wei Yani Fang Zexu Long Jiao Wang Fuyu An Jinqian Xu Tingting Xue Huangjie Qiu Yan Hua Guangshun Jiang 《Journal of Zoological Research》 2021年第3期1-20,共20页
Ecosystem engineers are organisms that alter the distribution of resources in the environment by creating,modifying,maintaining and/or destroying the habitat.They can affect the structure and function of the whole eco... Ecosystem engineers are organisms that alter the distribution of resources in the environment by creating,modifying,maintaining and/or destroying the habitat.They can affect the structure and function of the whole ecosystem furthermore.Burrowing engineers are an important group in ecosystem engineers as they play a critical role in soil translocation and habitat creation in various types of environment.However,few researchers have systematically summarized and analyzed the studies of burrowing engineers.We reviewing the existing ecological studies of burrowing engineer about their interaction with habitat through five directions:(1)soil turnover;(2)changing soil physicochemical properties;(3)changing plant community structure;(4)providing limited resources for commensal animals;and/or(5)affecting animal communities.The Chinese pangolin(Manis pentadactyla)is a typical example of burrowing mammals,in part(5),we focus on the interspecific relationships among burrow commensal species of Chinese pangolin.The engineering effects vary with environmental gradient,literature indicates that burrowing engineer play a stronger role in habitat transformation in the tropical and subtropical areas.The most common experiment method is comparative measurements(include different spatial and temporal scale),manipulative experiment is relatively few.We found that most of the engineering effects had positive feedback to the local ecosystem,increased plant abundance and resilience,increased biodiversity and consequently improved ecosystem functioning.With the global background of dramatic climate change and biodiversity loss in recent decades,we recommend future studies should improving knowledge of long-term engineering effects on population scale and landscape scale,exploring ecological cascades through trophic and engineering pathways,to better understand the attribute of the burrowing behavior of engineers to restore ecosystems and habitat creation.The review is presented as an aid to systematically expound the engineering effect of burrowing animals in the ecosystem,and provided new ideas and advice for planning and implementing conservation management. 展开更多
关键词 Burrowing engineer Burrow commensal species Chinese pangolin Ecosystem engineer Habitat modification Biodiversity
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A Review of the Ecology and Biology of Chinese Pangolin(Manis pentadactyla)
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作者 Jingyu YU Jianjun PENG +2 位作者 Jun ZENG Xilin YIN Jing ZHU 《Agricultural Science & Technology》 CAS 2015年第12期2770-2773,共4页
Chinese pangolin(Manis pentadactyla) has important value in Chinese medicine,economy and ecology.At present,Chinese pangolin is about to go extinct due to over hunting and habitat destruction,which has caused a dramat... Chinese pangolin(Manis pentadactyla) has important value in Chinese medicine,economy and ecology.At present,Chinese pangolin is about to go extinct due to over hunting and habitat destruction,which has caused a dramatic decline in the wild population.Breeding Chinese pangolin in captivity is very difficult and has not succeeded yet.This paper is to review the wild population,habitat,foraging,activity rhythm,breeding and physiology of Chinese pangolin.It provides theoretical basis of ecology and biology for the protection and artificial domestication of Chinese pangolin.Meanwhile,given the demands of protection,future research directions are suggested. 展开更多
关键词 Chinese Pangolin (Manis pentadactyla) ECOLOGY BIOLOGY PROTECTION
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A novel diagnostic gene region for distinguishing between two pest fruit flies:Bactrocera tryoni(Froggatt)and Bactrocera neohumeralis(Hardy)(Diptera:Tephritidae)
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作者 Melissa L.Starkie Elizabeth V.Fowler +9 位作者 Alexander M.Piper Xiaocheng Zhu Pauline Wyatt David Gopurenko Matt N.Krosch Francesca Strutt Karen F.Armstrong Hamish Patrick Mark K.Schutze Mark J.Blacket 《Insect Science》 SCIE CAS CSCD 2024年第4期1285-1295,共11页
Bactrocera tryoni and Bactrocera neohumeralis are morphologically similar sibling pest fruit fly species that possess different biological attributes,geographic distributions,and host ranges.The need to differentiate ... Bactrocera tryoni and Bactrocera neohumeralis are morphologically similar sibling pest fruit fly species that possess different biological attributes,geographic distributions,and host ranges.The need to differentiate between the two species is critical for accurate pest status assessment,management,biosecurity,and maintenance of reference colonies.While morphologically similar,adults may be separated based on subtle characters;however,some characters exhibit intraspecific variability,creating overlap between the two species.Additionally,there is currently no single molecular marker or rapid diagnostic assay that can reliably distinguish between B.neohumeralis and B.tryoni;therefore,ambiguous samples remain undiagnosed.Here we report the first molecular marker that can consistently distinguish between B.tryoni and B.neohumeralis.Our diagnostic region consists of two adjacent single nucleotide polymorphisms(SNPs)within the pangolin(pan)gene region.We confirmed the genotypes of each species are consistent across their distributional range,then developed a tetra-primer amplification refractory mutation system(ARMS)PCR assay for rapid diagnosis of the species.The assay utilizes four primers in multiplex,with two outer universal primers,and two internal primers:one designed to target two adjacent SNPs(AA)present in B.tryoni and the other targeting adjacent SNPs present in B.neohumeralis(GG).The assay accurately discriminates between the two species,but their SNP genotypes are shared with other nontarget tephritid fruit fly species.Therefore,this assay is most suited to adult diagnostics where species confirmation is necessary in determining ambiguous surveillance trap catches;maintaining pure colony lines;and in Sterile Insect Technique management responses. 展开更多
关键词 ARMS-PCR COLONY molecular diagnostics PANGOLIN tetra-primer
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Characterization of a pangolin SARS-CoV-2-related virus isolate that uses the human ACE2 receptor
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作者 Luo-Yuan Xia Xue-Feng Wang +26 位作者 Xiao-Ming Cui Yi-Ming Zhang Zhen-Fei Wang En-Tao Li Chang-Fa Fan Ke Song Yuan-Guo Li Run-Ze Ye Fang-Xu Li Dai-Yun Zhu Jie Zhang Zhuang-Zhuang Shi Ming-Zhu Zhang Liang-Jing Li Shi-Jing Shen Song Jin Ya-Wei Zhang Wei-Guang Fu Lin Zhao Wen-Hao Wang Tie-Cheng Wang You-Chun Wang Jia-Fu Jiang Yan-Ling Hu Na Jia Yu-Wei Gao Wu-Chun Cao 《Science China(Life Sciences)》 SCIE CAS CSCD 2024年第7期1502-1513,共12页
Various SARS-CoV-2-related coronaviruses have been increasingly identified in pangolins,showing a potential threat to humans.Here we report the infectivity and pathogenicity of the SARS-CoV-2-related virus,PCoV-GX/P2V... Various SARS-CoV-2-related coronaviruses have been increasingly identified in pangolins,showing a potential threat to humans.Here we report the infectivity and pathogenicity of the SARS-CoV-2-related virus,PCoV-GX/P2V,which was isolated from a Malayan pangolin(Manis javanica).PCoV-GX/P2V could grow in human hepatoma,colorectal adenocarcinoma cells,and human primary nasal epithelial cells.It replicated more efficiently in cells expressing human angiotensin-converting enzyme 2(hACE2)as SARS-CoV-2 did.After intranasal inoculation to the hACE2-transgenic mice,PCoV-GX/P2V not only replicated in nasal turbinate and lungs,but also caused interstitial pneumonia,characterized by infiltration of mixed inflammatory cells and multifocal alveolar hemorrhage.Existing population immunity established by SARS-CoV-2 infection and vaccination may not protect people from PCoV-GX/P2V infection.These findings further verify the hACE2 utility of PCoV-GX/P2V by in vivo experiments using authentic viruses and highlight the importance for intensive surveillance to prevent possible cross-species transmission. 展开更多
关键词 PANGOLIN SARS-CoV-2-related virus infectivity pathogenicity human cell lines human ACE2 receptor hACE2-transgenic mice
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Pangolin HKU4-related coronaviruses found in greater bamboo bats from southern China
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作者 Min Guo Kai Zhao +5 位作者 Xingwen Peng Xiangyang He Jin Deng Bo Wang Xinglou Yang Libiao Zhang 《Virologica Sinica》 SCIE CAS CSCD 2023年第6期868-876,共9页
Coronavirus(CoV)spillover originating from game animals,particularly pangolins,is currently a significant concern.Meanwhile,vigilance is urgently needed for coronaviruses carried by bats,which are known as natural res... Coronavirus(CoV)spillover originating from game animals,particularly pangolins,is currently a significant concern.Meanwhile,vigilance is urgently needed for coronaviruses carried by bats,which are known as natural reservoirs of many coronaviruses.In this study,we collected 729 anal swabs of 20 different bat species from nine locations in Yunnan and Guangdong provinces,southern China,in 2016 and 2017,and described the molecular characteristics and genetic diversity of alphacoronaviruses(αCoVs)and betacoronaviruses(βCoVs)found in these bats.Using RT-PCR,we identified 58(8.0%)bat CoVs in nine bat species from six locations.Furthermore,using the Illumina platform,we obtained two representative full-length genomes of the bat CoVs,namely TyRo-CoV-162275 and TyRo-CoV-162269.Sequence analysis showed that TyRo-CoV-162275 shared the highest identity with Malayan pangolin(Manis javanica)HKU4-related coronaviruses(MjHKU4r-CoVs)from Guangxi Province,whereas TyRo-CoV-162269 was closely related to HKU33-CoV discovered in a greater bamboo bat(Tylonycteris robustula)from Guizhou Province.Notably,TyRo-CoV-162275 has a putative furin protease cleavage site in its S protein and is likely to utilize human dipeptidyl peptidase-4(hDPP4)as a cell-entry receptor,similar to MERSCoV.To the best of our knowledge,this is the first report of a bat HKU4r-CoV strain containing a furin protease cleavage site.These findings expand our understanding of coronavirus geographic and host distributions. 展开更多
关键词 CORONAVIRUS Pangolin HKU4r-CoV Bat HKU4r-CoV Tylonycteris robustula Spillover risk
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